pycom17g05230

Uncharacterized ACR, COG1678

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0012172
Physical Location & Seq
Forward (+)
3615880 .. 3616850
971 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 882 bp
ATGGAAGCTTGTTTTCTTGCCTCAAATTCCTTCATCAAAACTGTTACTTCTCCCAACAGAAATTTCACTCAACTTCAATCGAAAAGAGTTGGGATTCCATCTTCAATAACATGTAGCCGTGGGGAGTCATCAACGCCGTCCAGTAGAGTTACGATCGATCACAAACCATCCATTGATGCAGATTGGCGATCATTCAGAGCAAGACTGGTAGCTGCAGAGAAATCAAGGTCAAAAGAGCCCACCTCAACTTCCTCACTTGATCTTGACACTGTGGTGGATCAACCTAAGCCCGTCACCGTTGGTGACAAATGGGCTCACGAAATCCACGAGCCTGAGAAGGGATGCCTACTCCTTGCAACCGACAAGCTCGACGGGGTCCACATATTCGAACGGACAGTCATTCTGGTGTTGTCCACTGCGCCATTGGGACCGTCGGGCATCATACTCAACCGGCCCTCCCTCATGTCGATCAAGGAAACGCGGTCGACCGCTCTGGACGTGGCGGGCACGTTCTCGGACCGGCCGTTGTTCTTCGGCGGGCCGTTGGAAGAGGGGTTATTCTTGGTGAGGCCCAAAGGGGGTGATGATGTAGTTGGGAGGAGTGGAGTTTTTGATGAGGTGATGAAGGGGTTGTACTATGGGACAAAGGAAAGTGTGGGGTGTGCGGCTGAGATGGTGAAGAGGAATATGGTTGGGCTTGGGGAGTTCAGGTTCTTTGATGGGTATTGTGGGTGGGAGAAGGAGCAGTTGAAGGATGAAATAAGATCTGGTTATTGGACTGTGGCAGCTTGTAGCCCAAGTGTGATTGATTTGAGGAGTGTGGGAAGTGTTGGGCTTTGGGAGAAGGTCCTTGGGCTTATGGGCCGTCGGAAGGTTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF179

Protein Analysis

294

Amino Acids

32.12

Weight (kDa)

8.48

Isoelectric Point (pI)

48.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF179 PF02622 128 - 288 1.1e-25 AlgH-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012172)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G29240 AT3G29240
fragaria_vesca FvH4_6g47750
malus_domestica MD09G1058900.v1.1 MD17G1054800.v1.1
prunus_persica Prupe.3G261300_v2.0.a1
pyrus_communis pycom17g05230
rosa_chinensis RchiOBHm_Chr2g0166901
rosa_laevigata RLG00000021677
rosa_multiflora Rmu_sc0002735.1_g000015 Rmu_sc0005344.1_g000008
rosa_roxburghii Rroxscaffold_2G00084030 Rroxscaffold_2G00084430
rosa_rugosa Rorug02G0526200
rosa_samantha Rh2AG593200 Rh2BG604700 Rh2CG575400 Rh2DG616200
rosa_wichuraiana Rw2G049380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 491
AccI GTMKAC 1 cut(s) 485
AccII CGCG 1 cut(s) 481
AciI CCGC 5 cut(s) 481, 489, 503, 537, 665
AclWI GGATC 1 cut(s) 285
AcoI YGGCCR 1 cut(s) 521
AcsI RAATTY 2 cut(s) 25, 61
AfaI GTAC 1 cut(s) 635
AfiI CCNNNNNNNGG 3 cut(s) 338, 578, 871
AflIII ACRYGT 1 cut(s) 110
AgsI TTSAA 3 cut(s) 77, 105, 751
AjiI CACGTC 1 cut(s) 499
AjuI GAANNNNNNNTTGG 2 cut(s) 47, 79
AleI CACNNNNGTG 1 cut(s) 272
AloI GAACNNNNNNTCC 2 cut(s) 695, 727
AluBI AGCT 4 cut(s) 8, 212, 367, 788
AluI AGCT 4 cut(s) 8, 212, 367, 788
AlwI GGATC 1 cut(s) 285
AoxI GGCC 5 cut(s) 452, 521, 539, 569, 862
ApeKI GCWGC 2 cut(s) 212, 785
ApoI RAATTY 2 cut(s) 25, 61
ArsI GACNNNNNNTTYG 2 cut(s) 381, 413
AspLEI GCGC 1 cut(s) 421
AspS9I GGNCC 8 cut(s) 376, 428, 453, 517, 539, 570, 847, 862
AsuHPI GGTGA 6 cut(s) 286, 314, 577, 593, 631, 688
AsuII TTCGAA 1 cut(s) 387
AvaII GGWCC 4 cut(s) 376, 428, 517, 847
BaeGI GKGCMC 1 cut(s) 509
BanII GRGCYC 2 cut(s) 240, 316
BarI GAAGNNNNNNTAC 2 cut(s) 617, 649
BauI CACGAG 1 cut(s) 326
BbvI GCAGC 2 cut(s) 199, 797
BccI CCATC 4 cut(s) 106, 175, 667, 713
BceAI ACGGC 5 cut(s) 102, 121, 508, 526, 849
BfmI CTRYAG 1 cut(s) 213
BglII AGATCT 1 cut(s) 764
BisI GCNGC 3 cut(s) 213, 666, 786
BlsI GCNGC 3 cut(s) 214, 667, 787
Bme18I GGWCC 4 cut(s) 376, 428, 517, 847
BmgBI CACGTC 1 cut(s) 499
BmgT120I GGNCC 8 cut(s) 376, 428, 453, 517, 539, 570, 847, 862
BmiI GGNNCC 2 cut(s) 377, 429
BmsI GCATC 3 cut(s) 166, 332, 447
BplI GAGNNNNNCTC 2 cut(s) 227, 259
Bpu10I CCTNAGC 1 cut(s) 285
Bpu14I TTCGAA 1 cut(s) 387
Bsa29I ATCGAT 1 cut(s) 156
BsaJI CCNNGG 2 cut(s) 118, 850
BsaXI ACNNNNNCTCC 4 cut(s) 440, 470, 695, 725
Bsc4I CCNNNNNNNGG 3 cut(s) 338, 578, 871
Bse118I RCCGGY 2 cut(s) 450, 519
Bse1I ACTGG 2 cut(s) 141, 210
BseCI ATCGAT 1 cut(s) 156
BseDI CCNNGG 2 cut(s) 118, 850
BseGI GGATG 3 cut(s) 167, 347, 760
BseLI CCNNNNNNNGG 3 cut(s) 338, 578, 871
BseMII CTCAG 2 cut(s) 324, 660
BseNI ACTGG 2 cut(s) 141, 210
BseRI GAGGAG 2 cut(s) 613, 829
BseSI GKGCMC 1 cut(s) 509
BseX3I CGGCCG 1 cut(s) 521
BseXI GCAGC 2 cut(s) 199, 797
Bsh1236I CGCG 1 cut(s) 481
Bsh1285I CGRYCG 4 cut(s) 156, 485, 489, 524
BshFI GGCC 5 cut(s) 454, 523, 541, 571, 864
BshVI ATCGAT 1 cut(s) 156
BsiEI CGRYCG 4 cut(s) 156, 485, 489, 524
BsiSI CCGG 2 cut(s) 451, 520
BslFI GGGAC 2 cut(s) 441, 655
BslI CCNNNNNNNGG 3 cut(s) 338, 578, 871
BsmFI GGGAC 2 cut(s) 441, 655
BsnI GGCC 5 cut(s) 454, 523, 541, 571, 864
Bsp119I TTCGAA 1 cut(s) 387
Bsp1286I GDGCHC 3 cut(s) 240, 316, 509
Bsp143I GATC 7 cut(s) 153, 157, 188, 259, 277, 468, 764
BspACI CCGC 5 cut(s) 481, 489, 503, 537, 665
BspANI GGCC 5 cut(s) 454, 523, 541, 571, 864
BspCNI CTCAG 2 cut(s) 325, 661
BspDI ATCGAT 1 cut(s) 156
BspFNI CGCG 1 cut(s) 481
BspLI GGNNCC 2 cut(s) 377, 429
BspMAI CTGCAG 1 cut(s) 217
BspPI GGATC 1 cut(s) 285
BspT104I TTCGAA 1 cut(s) 387
BsrBI CCGCTC 1 cut(s) 491
BsrFI RCCGGY 2 cut(s) 450, 519
BsrI ACTGG 2 cut(s) 141, 210
BssAI RCCGGY 2 cut(s) 450, 519
BssECI CCNNGG 2 cut(s) 118, 850
BssMI GATC 7 cut(s) 153, 157, 188, 259, 277, 468, 764
BssSI CACGAG 1 cut(s) 326
BssT1I CCWWGG 1 cut(s) 850
Bst2BI CACGAG 1 cut(s) 326
Bst4CI ACNGT 6 cut(s) 43, 271, 298, 397, 432, 781
Bst6I CTCTTC 2 cut(s) 543, 674
BstBI TTCGAA 1 cut(s) 387
BstC8I GCNNGC 2 cut(s) 505, 539
BstDEI CTNAG 3 cut(s) 285, 333, 669
BstDSI CCRYGG 1 cut(s) 118
BstF5I GGATG 3 cut(s) 167, 347, 760
BstFNI CGCG 1 cut(s) 481
BstHHI GCGC 1 cut(s) 421
BstKTI GATC 7 cut(s) 156, 160, 191, 262, 280, 471, 767
BstMBI GATC 7 cut(s) 153, 157, 188, 259, 277, 468, 764
BstMCI CGRYCG 4 cut(s) 156, 485, 489, 524
BstNSI RCATGY 1 cut(s) 114
BstSFI CTRYAG 1 cut(s) 213
BstSLI GKGCMC 1 cut(s) 509
BstUI CGCG 1 cut(s) 481
BstV1I GCAGC 2 cut(s) 199, 797
BstX2I RGATCY 1 cut(s) 764
BstYI RGATCY 1 cut(s) 764
BstZI CGGCCG 1 cut(s) 521
Bsu15I ATCGAT 1 cut(s) 156
BsuRI GGCC 5 cut(s) 454, 523, 541, 571, 864
BsuTUI ATCGAT 1 cut(s) 156
BtgI CCRYGG 1 cut(s) 118
BtrI CACGTC 1 cut(s) 499
BtsCI GGATG 3 cut(s) 167, 347, 760
BtsI GCAGTG 1 cut(s) 414
BtsIMutI CAGTG 2 cut(s) 267, 414
Cac8I GCNNGC 2 cut(s) 505, 539
CfoI GCGC 1 cut(s) 421
Cfr10I RCCGGY 2 cut(s) 450, 519
Cfr13I GGNCC 8 cut(s) 376, 428, 453, 517, 539, 570, 847, 862
ClaI ATCGAT 1 cut(s) 156
CpoI CGGWCCG 1 cut(s) 517
Csp6I GTAC 1 cut(s) 634
CspI CGGWCCG 1 cut(s) 517
CviAII CATG 2 cut(s) 111, 463
CviQI GTAC 1 cut(s) 634
DdeI CTNAG 3 cut(s) 285, 333, 669
DpnI GATC 7 cut(s) 155, 159, 190, 261, 279, 470, 766
DpnII GATC 7 cut(s) 153, 157, 188, 259, 277, 468, 764
EaeI YGGCCR 1 cut(s) 521
EagI CGGCCG 1 cut(s) 521
Eam1104I CTCTTC 2 cut(s) 543, 674
EarI CTCTTC 2 cut(s) 543, 674
EclXI CGGCCG 1 cut(s) 521
Eco130I CCWWGG 1 cut(s) 850
Eco24I GRGCYC 2 cut(s) 240, 316
Eco47I GGWCC 4 cut(s) 376, 428, 517, 847
Eco52I CGGCCG 1 cut(s) 521
EcoO109I RGGNCCY 1 cut(s) 847
EcoT14I CCWWGG 1 cut(s) 850
EcoT38I GRGCYC 2 cut(s) 240, 316
ErhI CCWWGG 1 cut(s) 850
FaeI CATG 2 cut(s) 114, 466
FaiI YATR 7 cut(s) 112, 383, 443, 464, 639, 689, 860
FaqI GGGAC 2 cut(s) 441, 655
FatI CATG 2 cut(s) 110, 462
FauI CCCGC 2 cut(s) 496, 530
FblI GTMKAC 1 cut(s) 485
Fnu4HI GCNGC 3 cut(s) 213, 666, 786
FokI GGATG 3 cut(s) 154, 354, 767
FriOI GRGCYC 2 cut(s) 240, 316
Fsp4HI GCNGC 3 cut(s) 213, 666, 786
GlaI GCGC 1 cut(s) 420
GluI GCNGC 3 cut(s) 213, 666, 786
HaeIII GGCC 5 cut(s) 454, 523, 541, 571, 864
HapII CCGG 2 cut(s) 451, 520
HhaI GCGC 1 cut(s) 421
Hin1II CATG 2 cut(s) 114, 466
Hin6I GCGC 1 cut(s) 419
HinP1I GCGC 1 cut(s) 419
HincII GTYRAC 1 cut(s) 486
HindII GTYRAC 1 cut(s) 486
HindIII AAGCTT 1 cut(s) 6
HinfI GANTC 2 cut(s) 94, 125
HpaII CCGG 2 cut(s) 451, 520
HphI GGTGA 6 cut(s) 286, 314, 577, 593, 631, 688
Hpy166II GTNNAC 3 cut(s) 379, 414, 486
Hpy188I TCNGA 3 cut(s) 197, 517, 870
Hpy188III TCNNGA 3 cut(s) 263, 317, 494
Hpy8I GTNNAC 3 cut(s) 379, 414, 486
Hpy99I CGWCG 3 cut(s) 374, 436, 870
HpyAV CCTTC 7 cut(s) 40, 331, 619, 733, 745, 838, 865
HpyCH4III ACNGT 6 cut(s) 43, 271, 298, 397, 432, 781
HpyCH4IV ACGT 2 cut(s) 498, 509
HpyCH4V TGCA 3 cut(s) 179, 215, 356
HpyF3I CTNAG 3 cut(s) 285, 333, 669
HpySE526I ACGT 2 cut(s) 498, 509
Hsp92II CATG 2 cut(s) 114, 466
HspAI GCGC 1 cut(s) 419
Kzo9I GATC 7 cut(s) 153, 157, 188, 259, 277, 468, 764
LmnI GCTCC 1 cut(s) 742
LpnPI CCDG 9 cut(s) 154, 191, 345, 389, 464, 479, 533, 694, 753
Lsp1109I GCAGC 2 cut(s) 199, 797
LweI GCATC 3 cut(s) 166, 332, 447
MaeII ACGT 2 cut(s) 498, 509
MaeIII GTNAC 4 cut(s) 43, 148, 292, 302
MalI GATC 7 cut(s) 155, 159, 190, 261, 279, 470, 766
MbiI CCGCTC 1 cut(s) 491
MboI GATC 7 cut(s) 153, 157, 188, 259, 277, 468, 764
MboII GAAGA 4 cut(s) 93, 523, 560, 691
MflI RGATCY 1 cut(s) 764
MhlI GDGCHC 3 cut(s) 240, 316, 509
MluCI AATT 2 cut(s) 25, 61
MlyI GAGTC 1 cut(s) 134
MmeI TCCRAC 2 cut(s) 525, 848
MslI CAYNNNNRTG 2 cut(s) 272, 404
MspI CCGG 2 cut(s) 451, 520
MvnI CGCG 1 cut(s) 481
NdeII GATC 7 cut(s) 153, 157, 188, 259, 277, 468, 764
NlaIII CATG 2 cut(s) 114, 466
NlaIV GGNNCC 2 cut(s) 377, 429
NmuCI GTSAC 2 cut(s) 292, 302
NspI RCATGY 1 cut(s) 114
NspV TTCGAA 1 cut(s) 387
OliI CACNNNNGTG 1 cut(s) 272
PciI ACATGT 1 cut(s) 110
PcsI WCGNNNNNNNCGW 2 cut(s) 324, 521
PfeI GAWTC 1 cut(s) 94
PflFI GACNNNGTC 1 cut(s) 374
PkrI GCNGC 3 cut(s) 214, 667, 787
Ple19I CGATCG 1 cut(s) 156
PleI GAGTC 1 cut(s) 133
PpsI GAGTC 1 cut(s) 133
PpuMI RGGWCCY 1 cut(s) 847
PscI ACATGT 1 cut(s) 110
Psp5II RGGWCCY 1 cut(s) 847
PspN4I GGNNCC 2 cut(s) 377, 429
PspPI GGNCC 8 cut(s) 376, 428, 453, 517, 539, 570, 847, 862
PspPPI RGGWCCY 1 cut(s) 847
PstI CTGCAG 1 cut(s) 217
PsuI RGATCY 1 cut(s) 764
PsyI GACNNNGTC 1 cut(s) 374
PvuI CGATCG 1 cut(s) 156
RsaI GTAC 1 cut(s) 635
RsaNI GTAC 1 cut(s) 634
RseI CAYNNNNRTG 2 cut(s) 272, 404
Rsr2I CGGWCCG 1 cut(s) 517
RsrII CGGWCCG 1 cut(s) 517
SalI GTCGAC 1 cut(s) 484
SatI GCNGC 3 cut(s) 213, 666, 786
Sau3AI GATC 7 cut(s) 153, 157, 188, 259, 277, 468, 764
Sau96I GGNCC 8 cut(s) 376, 428, 453, 517, 539, 570, 847, 862
SchI GAGTC 1 cut(s) 134
SduI GDGCHC 3 cut(s) 240, 316, 509
SfaNI GCATC 3 cut(s) 166, 332, 447
SfcI CTRYAG 1 cut(s) 213
SfuI TTCGAA 1 cut(s) 387
SinI GGWCC 4 cut(s) 376, 428, 517, 847
SmiMI CAYNNNNRTG 2 cut(s) 272, 404
Sse9I AATT 2 cut(s) 25, 61
SsiI CCGC 5 cut(s) 481, 489, 503, 537, 665
StyI CCWWGG 1 cut(s) 850
TaaI ACNGT 6 cut(s) 43, 271, 298, 397, 432, 781
TaiI ACGT 2 cut(s) 501, 512
TaqI TCGA 6 cut(s) 80, 156, 369, 387, 467, 485
TasI AATT 2 cut(s) 25, 61
TatI WGTACW 1 cut(s) 633
TauI GCSGC 1 cut(s) 668
TfiI GAWTC 1 cut(s) 94
TscAI CASTG 2 cut(s) 274, 421
TseFI GTSAC 2 cut(s) 292, 302
TseI GCWGC 2 cut(s) 212, 785
Tsp45I GTSAC 2 cut(s) 292, 302
TspDTI ATGAA 3 cut(s) 22, 638, 771
TspGWI ACGGA 1 cut(s) 406
TspRI CASTG 2 cut(s) 274, 421
Tth111I GACNNNGTC 1 cut(s) 374
VpaK11BI GGWCC 4 cut(s) 376, 428, 517, 847
XapI RAATTY 2 cut(s) 25, 61
XceI RCATGY 1 cut(s) 114
XcmI CCANNNNNNNNNTGG 1 cut(s) 421
XmiI GTMKAC 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.