pycom17g07120

tryptophan synthase beta chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0016013
Physical Location & Seq
Forward (+)
5132645 .. 5135189
2545 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1509 bp
ATGGCCACTCAAAGTCTCTTCACTGCAAATTCAATTCACACTAAACCTTCATCATCCCCACACACTAAAGTTGAGAAGAAATGGTCTGGATTTATCTCACTTGATAGAAAGCCTATGCATATGAGGCTTTCAAATGGCTGTAGAGCAAGAGCATCTCTCAATGCCGATTCGAAGTCAATTGAAATCCCTCGACAGTGGTATAACTTGATTGCAGACCTTCCTGTAAAACCACCTCCTCCATTGCATCCCAAGACTTTTGAACCGATTAAACCTGAAGATTTGTCTCCTCTATTTCCCGATGAGTTGATTAAGCAGGAGGCAAGCAACGAAAGATTCATAGATATCCCAGATGAAGTTCGTGATGTATACAAGCTTTGGCGCCCATCACCTCTCATTAGGGCCAAGAGGTTGGAGAAGCTTCTTGACACACCCGCCAGAATTTACTACAAGTATGAAGGTGTCAGCCCGGCTGGATCACACAAGCCCAACTCTGCAGTTCCACAAGTGTGGTATAATGCACAAGAAGGAATCAAGAAGATCGTGACAGAAACGGGAGCTGGCCAATGGGGAAGTGCATTGGCTTTTGCTTGCACCTTATTTGATGTTGAGTGTGAGGTGTGGCAAGTGCGTGCATCTTTTGATCAGAAACCATATCGCAGATTGATGATGCAAACTTGGGGTGCAAAGGTATACCCATCTCCATCCACCCTAACCGAAGCAGGTAGAAAAATCCTTCGAATCGATCCATCCAGCCCCGGAAGTTTGGGAATAGCTATTTCTGAAGCCGTGGAGATTGCAGCTATGAATGGTGATACTAAATACTGTCTAGGAAGTGTTCTCAACCATGTTTTGCTTCACCAGACGGTAATAGGAGAGGAGTGTCTAAAACAAATGGAGGCGATAGGTGAAGTCCCGGATGTTATCATAGGATGTACTGGTGGAGGATCCAATTTTGCAGGGCTCAGTTTCCCATTCATCAGGGAGAAGCTGAAAGGGAAAATGAACCCTGTAATTAGAGCAGTTGAACCTGCAGCATGTCCTTCGTTAACTAAAGGCGTATACGCGTATGATTATGGCGATACAGCGGGGATGACTCCACTAATGAAGATGCATACGCTTGGGCACGACTTCATCCCTGACCCAATTCATGCTGGGGGATTGCGCTACCATGGTATGTCACCGTTAATTTCACACGTCTACGAACTGGGTTACATGGAAGCAATTTCAATTCCCCAGATTGAGTGCTTTCAAGGTGCTATAAAATTTGCTAGAACCGAAGGATTGATACCAGCGCCAGAGCCAACACATGCCATAGCTGCCACCATCAGGGAAGCTCAGAAATGTCGAGAGACCGGAGAAGCCAAAGTGATTCTCACAGCAATGTGTGGGCATGGACATTTTGATTTGCCAGCTTATGAGAATTATCTGCAAGGAAATCTGGTGGATTTGTCATTTGAAGAGGAGAAGATTCAAGCATCATTGGCTAGTGTTCCTAAAGTAGGTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00400 ko01100 ko01110 ko01130 ko01230 map00260 map00400 map01100 map01110 map01130 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

503

Amino Acids

55.31

Weight (kDa)

6.95

Isoelectric Point (pI)

45.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 125 - 463 1.1e-37 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016013)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38530
fragaria_vesca FvH4_6g45680
malus_domestica MD17G1071900.v1.1
prunus_persica Prupe.3G243000_v2.0.a1
pyrus_communis pycom17g07120
rosa_chinensis RchiOBHm_Chr2g0163831
rosa_laevigata RLG00000021419
rosa_multiflora Rmu_sc0005917.1_g000003
rosa_roxburghii Rroxscaffold_2G00086980
rosa_rugosa Rorug02G0504800
rosa_samantha Rh2AG572800 Rh2BG583500 Rh2CG554400 Rh2DG593100
rosa_wichuraiana Rw2G047360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 710, 1036
AccB1I GGYRCC 1 cut(s) 378
AccI GTMKAC 4 cut(s) 366, 690, 1059, 1197
AccII CGCG 1 cut(s) 1064
AciI CCGC 2 cut(s) 432, 1085
AclWI GGATC 4 cut(s) 481, 737, 939, 952
AcoI YGGCCR 2 cut(s) 3, 559
AcsI RAATTY 3 cut(s) 28, 438, 1262
AcuI CTGAAG 2 cut(s) 294, 801
AcyI GRCGYC 1 cut(s) 379
AfaI GTAC 1 cut(s) 934
AfiI CCNNNNNNNGG 2 cut(s) 1326, 1499
AflIII ACRYGT 2 cut(s) 1062, 1192
AgsI TTSAA 9 cut(s) 33, 132, 182, 260, 1025, 1227, 1250, 1457, 1472
AjiI CACGTC 1 cut(s) 1195
AleI CACNNNNGTG 1 cut(s) 505
AluBI AGCT 9 cut(s) 373, 418, 557, 773, 800, 988, 1316, 1334, 1412
AluI AGCT 9 cut(s) 373, 418, 557, 773, 800, 988, 1316, 1334, 1412
Alw26I GTCTC 3 cut(s) 20, 288, 1343
AlwI GGATC 4 cut(s) 481, 737, 939, 952
AoxI GGCC 3 cut(s) 3, 399, 559
ApeKI GCWGC 3 cut(s) 797, 1031, 1316
ApoI RAATTY 3 cut(s) 28, 438, 1262
AspLEI GCGC 3 cut(s) 381, 1164, 1294
AspS9I GGNCC 1 cut(s) 399
AsuC2I CCSGG 3 cut(s) 467, 756, 914
AsuHPI GGTGA 5 cut(s) 378, 821, 848, 917, 1170
AsuII TTCGAA 2 cut(s) 170, 736
BaeGI GKGCMC 1 cut(s) 1125
BalI TGGCCA 2 cut(s) 5, 561
BamHI GGATCC 1 cut(s) 944
BanI GGYRCC 1 cut(s) 378
BanII GRGCYC 1 cut(s) 963
BarI GAAGNNNNNNTAC 2 cut(s) 1269, 1301
BbvI GCAGC 3 cut(s) 809, 1043, 1303
BccI CCATC 5 cut(s) 391, 703, 709, 754, 1331
BceAI ACGGC 1 cut(s) 770
BcgI CGANNNNNNTGC 2 cut(s) 1023, 1057
BclI TGATCA 1 cut(s) 640
BcnI CCSGG 3 cut(s) 467, 756, 914
BcoDI GTCTC 3 cut(s) 20, 288, 1343
BfaI CTAG 3 cut(s) 827, 1269, 1485
BfmI CTRYAG 3 cut(s) 139, 492, 1029
BfoI RGCGCY 2 cut(s) 382, 1295
BfuAI ACCTGC 2 cut(s) 710, 1036
BisI GCNGC 3 cut(s) 798, 1032, 1317
BlsI GCNGC 3 cut(s) 799, 1033, 1318
Bme1390I CCNGG 3 cut(s) 467, 756, 914
BmgBI CACGTC 1 cut(s) 1195
BmgT120I GGNCC 1 cut(s) 399
BmiI GGNNCC 2 cut(s) 380, 946
BmrFI CCNGG 3 cut(s) 467, 756, 914
BmrI ACTGGG 1 cut(s) 1214
BmsI GCATC 6 cut(s) 161, 253, 641, 657, 1098, 1484
BmuI ACTGGG 1 cut(s) 1214
BplI GAGNNNNNCTC 2 cut(s) 141, 173
Bpu14I TTCGAA 2 cut(s) 170, 736
BpuMI CCSGG 3 cut(s) 467, 756, 914
Bsa29I ATCGAT 1 cut(s) 741
BsaHI GRCGYC 1 cut(s) 379
BsaI GGTCTC 1 cut(s) 1343
BsaJI CCNNGG 3 cut(s) 754, 786, 1168
BsaWI WCCGGW 1 cut(s) 1352
Bsc4I CCNNNNNNNGG 2 cut(s) 1326, 1499
Bse1I ACTGG 2 cut(s) 940, 1209
Bse3DI GCAATG 2 cut(s) 239, 1386
BseCI ATCGAT 1 cut(s) 741
BseDI CCNNGG 3 cut(s) 754, 786, 1168
BseGI GGATG 8 cut(s) 53, 244, 701, 746, 922, 935, 1095, 1131
BseLI CCNNNNNNNGG 2 cut(s) 1326, 1499
BseMI GCAATG 2 cut(s) 239, 1386
BseMII CTCAG 2 cut(s) 976, 1349
BseNI ACTGG 2 cut(s) 940, 1209
BseRI GAGGAG 4 cut(s) 225, 276, 890, 1475
BseSI GKGCMC 1 cut(s) 1125
BseXI GCAGC 3 cut(s) 809, 1043, 1303
BseYI CCCAGC 1 cut(s) 1151
Bsh1236I CGCG 1 cut(s) 1064
BshFI GGCC 3 cut(s) 5, 401, 561
BshNI GGYRCC 1 cut(s) 378
BshVI ATCGAT 1 cut(s) 741
BsiSI CCGG 4 cut(s) 467, 756, 914, 1353
BslFI GGGAC 1 cut(s) 896
BslI CCNNNNNNNGG 2 cut(s) 1326, 1499
BsmAI GTCTC 3 cut(s) 20, 288, 1343
BsmFI GGGAC 1 cut(s) 896
BsnI GGCC 3 cut(s) 5, 401, 561
Bso31I GGTCTC 1 cut(s) 1343
Bsp119I TTCGAA 2 cut(s) 170, 736
Bsp1286I GDGCHC 2 cut(s) 963, 1125
Bsp143I GATC 5 cut(s) 473, 537, 640, 742, 944
Bsp19I CCATGG 1 cut(s) 1168
BspACI CCGC 2 cut(s) 432, 1085
BspANI GGCC 3 cut(s) 5, 401, 561
BspCNI CTCAG 2 cut(s) 975, 1348
BspDI ATCGAT 1 cut(s) 741
BspFNI CGCG 1 cut(s) 1064
BspLI GGNNCC 2 cut(s) 380, 946
BspMAI CTGCAG 2 cut(s) 496, 1033
BspMI ACCTGC 2 cut(s) 710, 1036
BspPI GGATC 4 cut(s) 481, 737, 939, 952
BspT104I TTCGAA 2 cut(s) 170, 736
BspT107I GGYRCC 1 cut(s) 378
BspTNI GGTCTC 1 cut(s) 1343
BsrDI GCAATG 2 cut(s) 239, 1386
BsrI ACTGG 2 cut(s) 940, 1209
BssECI CCNNGG 3 cut(s) 754, 786, 1168
BssMI GATC 5 cut(s) 473, 537, 640, 742, 944
BssNAI GTATAC 3 cut(s) 367, 691, 1060
BssNI GRCGYC 1 cut(s) 379
BssT1I CCWWGG 1 cut(s) 1168
Bst1107I GTATAC 3 cut(s) 367, 691, 1060
Bst4CI ACNGT 4 cut(s) 195, 824, 865, 1182
Bst6I CTCTTC 2 cut(s) 23, 1452
BstACI GRCGYC 1 cut(s) 379
BstBI TTCGAA 2 cut(s) 170, 736
BstC8I GCNNGC 5 cut(s) 322, 559, 589, 630, 1410
BstDEI CTNAG 2 cut(s) 962, 1335
BstDSI CCRYGG 2 cut(s) 786, 1168
BstENI CCTNNNNNAGG 1 cut(s) 1497
BstF5I GGATG 8 cut(s) 53, 244, 701, 746, 922, 935, 1095, 1131
BstFNI CGCG 1 cut(s) 1064
BstH2I RGCGCY 2 cut(s) 382, 1295
BstHHI GCGC 3 cut(s) 381, 1164, 1294
BstKTI GATC 5 cut(s) 476, 540, 643, 745, 947
BstMAI GTCTC 3 cut(s) 20, 288, 1343
BstMBI GATC 5 cut(s) 473, 537, 640, 742, 944
BstMWI GCNNNNNNNGC 3 cut(s) 124, 1316, 1481
BstNSI RCATGY 2 cut(s) 1038, 1310
BstSCI CCNGG 3 cut(s) 465, 754, 912
BstSFI CTRYAG 3 cut(s) 139, 492, 1029
BstSLI GKGCMC 1 cut(s) 1125
BstUI CGCG 1 cut(s) 1064
BstV1I GCAGC 3 cut(s) 809, 1043, 1303
BstX2I RGATCY 1 cut(s) 944
BstXI CCANNNNNNTGG 2 cut(s) 409, 507
BstYI RGATCY 1 cut(s) 944
BstZ17I GTATAC 3 cut(s) 367, 691, 1060
Bsu15I ATCGAT 1 cut(s) 741
BsuRI GGCC 3 cut(s) 5, 401, 561
BsuTUI ATCGAT 1 cut(s) 741
BtgI CCRYGG 2 cut(s) 786, 1168
BtrI CACGTC 1 cut(s) 1195
BtsCI GGATG 8 cut(s) 53, 244, 701, 746, 922, 935, 1095, 1131
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 2 cut(s) 21, 200
BveI ACCTGC 2 cut(s) 710, 1036
Cac8I GCNNGC 5 cut(s) 322, 559, 589, 630, 1410
CfoI GCGC 3 cut(s) 381, 1164, 1294
Cfr13I GGNCC 1 cut(s) 399
ClaI ATCGAT 1 cut(s) 741
Csp6I GTAC 1 cut(s) 933
CviAII CATG 7 cut(s) 845, 1035, 1148, 1169, 1213, 1307, 1391
CviQI GTAC 1 cut(s) 933
DdeI CTNAG 2 cut(s) 962, 1335
DinI GGCGCC 1 cut(s) 380
DpnI GATC 5 cut(s) 475, 539, 642, 744, 946
DpnII GATC 5 cut(s) 473, 537, 640, 742, 944
EaeI YGGCCR 2 cut(s) 3, 559
Eam1104I CTCTTC 2 cut(s) 23, 1452
EarI CTCTTC 2 cut(s) 23, 1452
Eco130I CCWWGG 1 cut(s) 1168
Eco24I GRGCYC 1 cut(s) 963
Eco31I GGTCTC 1 cut(s) 1343
Eco32I GATATC 1 cut(s) 343
Eco57I CTGAAG 2 cut(s) 294, 801
EcoNI CCTNNNNNAGG 1 cut(s) 1497
EcoRV GATATC 1 cut(s) 343
EcoT14I CCWWGG 1 cut(s) 1168
EcoT22I ATGCAT 2 cut(s) 120, 1113
EcoT38I GRGCYC 1 cut(s) 963
EgeI GGCGCC 1 cut(s) 380
EheI GGCGCC 1 cut(s) 380
ErhI CCWWGG 1 cut(s) 1168
FaeI CATG 7 cut(s) 848, 1038, 1151, 1172, 1216, 1310, 1394
FaqI GGGAC 1 cut(s) 896
FatI CATG 7 cut(s) 844, 1034, 1147, 1168, 1212, 1306, 1390
FauI CCCGC 2 cut(s) 439, 1078
FauNDI CATATG 1 cut(s) 120
FbaI TGATCA 1 cut(s) 640
FblI GTMKAC 4 cut(s) 366, 690, 1059, 1197
Fnu4HI GCNGC 3 cut(s) 798, 1032, 1317
FokI GGATG 8 cut(s) 40, 231, 688, 733, 929, 942, 1102, 1118
FriOI GRGCYC 1 cut(s) 963
Fsp4HI GCNGC 3 cut(s) 798, 1032, 1317
FspBI CTAG 3 cut(s) 827, 1269, 1485
GlaI GCGC 3 cut(s) 380, 1163, 1293
GluI GCNGC 3 cut(s) 798, 1032, 1317
GsaI CCCAGC 1 cut(s) 1155
HaeII RGCGCY 2 cut(s) 382, 1295
HaeIII GGCC 3 cut(s) 5, 401, 561
HapII CCGG 4 cut(s) 467, 756, 914, 1353
HhaI GCGC 3 cut(s) 381, 1164, 1294
Hin1I GRCGYC 1 cut(s) 379
Hin1II CATG 7 cut(s) 848, 1038, 1151, 1172, 1216, 1310, 1394
Hin6I GCGC 3 cut(s) 379, 1162, 1292
HinP1I GCGC 3 cut(s) 379, 1162, 1292
HincII GTYRAC 1 cut(s) 1047
HindII GTYRAC 1 cut(s) 1047
HindIII AAGCTT 2 cut(s) 371, 416
HinfI GANTC 7 cut(s) 167, 333, 528, 738, 1093, 1369, 1468
HpaI GTTAAC 1 cut(s) 1047
HpaII CCGG 4 cut(s) 467, 756, 914, 1353
HphI GGTGA 5 cut(s) 378, 821, 848, 917, 1170
Hpy166II GTNNAC 5 cut(s) 367, 691, 1047, 1060, 1198
Hpy188I TCNGA 3 cut(s) 645, 781, 1338
Hpy188III TCNNGA 7 cut(s) 87, 296, 359, 422, 532, 541, 1346
Hpy8I GTNNAC 5 cut(s) 367, 691, 1047, 1060, 1198
HpyAV CCTTC 7 cut(s) 57, 227, 449, 518, 743, 1050, 1271
HpyCH4III ACNGT 4 cut(s) 195, 824, 865, 1182
HpyCH4IV ACGT 1 cut(s) 1194
HpyF10VI GCNNNNNNNGC 3 cut(s) 124, 1316, 1481
HpyF3I CTNAG 2 cut(s) 962, 1335
HpySE526I ACGT 1 cut(s) 1194
Hsp92I GRCGYC 1 cut(s) 379
Hsp92II CATG 7 cut(s) 848, 1038, 1151, 1172, 1216, 1310, 1394
HspAI GCGC 3 cut(s) 379, 1162, 1292
KasI GGCGCC 1 cut(s) 378
Ksp22I TGATCA 1 cut(s) 640
KspAI GTTAAC 1 cut(s) 1047
Kzo9I GATC 5 cut(s) 473, 537, 640, 742, 944
LmnI GCTCC 1 cut(s) 554
Lsp1109I GCAGC 3 cut(s) 809, 1043, 1303
LweI GCATC 6 cut(s) 161, 253, 641, 657, 1098, 1484
MaeI CTAG 3 cut(s) 827, 1269, 1485
MaeII ACGT 1 cut(s) 1194
MaeIII GTNAC 3 cut(s) 541, 1176, 1208
MalI GATC 5 cut(s) 475, 539, 642, 744, 946
MboI GATC 5 cut(s) 473, 537, 640, 742, 944
MboII GAAGA 7 cut(s) 10, 88, 287, 547, 1117, 1469, 1477
MfeI CAATTG 1 cut(s) 177
MflI RGATCY 1 cut(s) 944
MhlI GDGCHC 2 cut(s) 963, 1125
MlsI TGGCCA 2 cut(s) 5, 561
MluI ACGCGT 1 cut(s) 1062
MluNI TGGCCA 2 cut(s) 5, 561
Mly113I GGCGCC 1 cut(s) 379
MlyI GAGTC 1 cut(s) 1087
MmeI TCCRAC 1 cut(s) 390
Mox20I TGGCCA 2 cut(s) 5, 561
Mph1103I ATGCAT 2 cut(s) 120, 1113
MscI TGGCCA 2 cut(s) 5, 561
MseI TTAA 4 cut(s) 267, 309, 1046, 1184
MslI CAYNNNNRTG 2 cut(s) 505, 1379
Msp20I TGGCCA 2 cut(s) 5, 561
MspA1I CMGCKG 1 cut(s) 1085
MspI CCGG 4 cut(s) 467, 756, 914, 1353
MspR9I CCNGG 3 cut(s) 467, 756, 914
MunI CAATTG 1 cut(s) 177
MvnI CGCG 1 cut(s) 1064
MwoI GCNNNNNNNGC 3 cut(s) 124, 1316, 1481
NarI GGCGCC 1 cut(s) 379
NciI CCSGG 3 cut(s) 467, 756, 914
NcoI CCATGG 1 cut(s) 1168
NdeI CATATG 1 cut(s) 120
NdeII GATC 5 cut(s) 473, 537, 640, 742, 944
NlaIII CATG 7 cut(s) 848, 1038, 1151, 1172, 1216, 1310, 1394
NlaIV GGNNCC 2 cut(s) 380, 946
NmuCI GTSAC 2 cut(s) 541, 1176
NsiI ATGCAT 2 cut(s) 120, 1113
NspI RCATGY 2 cut(s) 1038, 1310
NspV TTCGAA 2 cut(s) 170, 736
OliI CACNNNNGTG 1 cut(s) 505
PfeI GAWTC 6 cut(s) 167, 333, 528, 738, 1369, 1468
PfoI TCCNGGA 1 cut(s) 912
PkrI GCNGC 3 cut(s) 799, 1033, 1318
PleI GAGTC 1 cut(s) 1087
PluTI GGCGCC 1 cut(s) 382
PpsI GAGTC 1 cut(s) 1087
PspFI CCCAGC 1 cut(s) 1151
PspN4I GGNNCC 2 cut(s) 380, 946
PspPI GGNCC 1 cut(s) 399
PstI CTGCAG 2 cut(s) 496, 1033
PsuI RGATCY 1 cut(s) 944
RsaI GTAC 1 cut(s) 934
RsaNI GTAC 1 cut(s) 933
RseI CAYNNNNRTG 2 cut(s) 505, 1379
SaqAI TTAA 4 cut(s) 267, 309, 1046, 1184
SatI GCNGC 3 cut(s) 798, 1032, 1317
Sau3AI GATC 5 cut(s) 473, 537, 640, 742, 944
Sau96I GGNCC 1 cut(s) 399
SchI GAGTC 1 cut(s) 1087
ScrFI CCNGG 3 cut(s) 467, 756, 914
SduI GDGCHC 2 cut(s) 963, 1125
SfaNI GCATC 6 cut(s) 161, 253, 641, 657, 1098, 1484
SfcI CTRYAG 3 cut(s) 139, 492, 1029
SfoI GGCGCC 1 cut(s) 380
SfuI TTCGAA 2 cut(s) 170, 736
SmiMI CAYNNNNRTG 2 cut(s) 505, 1379
SsiI CCGC 2 cut(s) 432, 1085
SspDI GGCGCC 1 cut(s) 378
SspMI CTAG 3 cut(s) 827, 1269, 1485
StyD4I CCNGG 3 cut(s) 465, 754, 912
StyI CCWWGG 1 cut(s) 1168
TaaI ACNGT 4 cut(s) 195, 824, 865, 1182
TaiI ACGT 1 cut(s) 1197
TaqI TCGA 5 cut(s) 170, 190, 736, 741, 1345
TatI WGTACW 1 cut(s) 932
TfiI GAWTC 6 cut(s) 167, 333, 528, 738, 1369, 1468
Tru1I TTAA 4 cut(s) 267, 309, 1046, 1184
Tru9I TTAA 4 cut(s) 267, 309, 1046, 1184
TscAI CASTG 2 cut(s) 28, 200
TseFI GTSAC 2 cut(s) 541, 1176
TseI GCWGC 3 cut(s) 797, 1031, 1316
Tsp45I GTSAC 2 cut(s) 541, 1176
TspRI CASTG 2 cut(s) 28, 200
XagI CCTNNNNNAGG 1 cut(s) 1497
XapI RAATTY 3 cut(s) 28, 438, 1262
XceI RCATGY 2 cut(s) 1038, 1310
XmiI GTMKAC 4 cut(s) 366, 690, 1059, 1197
XspI CTAG 3 cut(s) 827, 1269, 1485
Zsp2I ATGCAT 2 cut(s) 120, 1113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.