Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr17

Orthogroup ID

OG0014465

Physical Location & Seq

Genomic Coordinates Forward (+)

5986088 .. 5989478

Gene Structure 3391 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3303 bp

ATGATGCTTCACAGCCAGAGTTACAGCTCCATGGAATTAGGGCAAGAAAGTTTCCAGAGCTCAATTCACAGTCGTCGCACTTTTTCACCAAGTAAGCTTAGAAACCCCCATCAATGTCCTTTCAGAGCTAGGGCTTTTCTAGAATTTAACTTACCCAATTCTCCGAATTTCGAAACGATTGTGAATTCACGAGCTCAGAAACAGAATTTGAGAGGTTCTAGGGTTTTTGTAGGGTTTAAGATTCAGTGTCATTCGAAATCTTCGGTTTTGCCCACGAAAGTTTCTTCGATTAACGGTAAGAAGAAGGGGTATGGAGGCGTGTTGTCTTCGATTTTGCGGTCTATAGAGTCCGAAAATGATTTTGAAAAGACCCTTAATTCGTTAGGTGAAAATTTGAATCCGAAAGAACAGACTAATTCTCAAGGAACAAAAAAGCTGGGAAAGGGTTCTGCGTGTTTTCGAGTGGTTCAAGGCACAGAAAGAGTATTGGGTAGGGCTCAGAAGTGGGATGAATTGAGGCTTTGTTGGATTGAAATGGCGAAAAGGGGTGTTCTGCCAACAAACAACACGTATGCAATGCTCGTTGATGTGTATGGGAAAGCCGGCCTTGTGAAGGAAGCACTTTTGTGGATTAAGCACATGAAACTGAGGGGAATGTTTCCGGATGATGTTACAATGAATACGGTTGTTCGGGCCTTGAAGGATGCAGGAGAGTTTGATAGGGCTGATAAGTTTTATAAGGATTGGTGCACCGGGAAGATTGAGCTCGATGAACTAGATTTGAATTCAATGGGTGACTCTGTGAATAGTTATGGCTCAGAGCCTATTAGTTTTAAGCATTTCTTGTCCACTGAACTTTTCAAGACAGGTGGGAGAATTCCCACATCAAAGGTTACGACCTCATTGGATACACAGAACTCTGTTCAAAAACCACGACAGGCAACGACATATAATGCTTTGATTGATTTGTACGGGAAGGCAGGGCGTCTGGATGATGCAGCGAATGTGTTTGGAGAAATGATGAAATCTGGGGTCCCAATGGATGTCATAACTTTCAACACTATGATCTTTACTTGTGGAAGTAATGGGCATTTGTCGGAAGCGGAAACTTTGCTTAGCAAGATGGAAGAAAGGGGAATTTCTCCTGACACAAGAACTTATAACATTTTTCTATCTCTGTATGCCGATATGGGGAACATTGATGCTGCTCTGAACTGTTATAGGAAGATCAGAGAGGTGGGTCTTTCCCCAGATGTCGTCTCTCACAGGACTATTCTTCACGTGTTATGTGAGAGAAACATGGTCCGAGAAGTGGAGACTGTGATTCAGGATATGGAGAAATCTGGTGTGCGTGTCGATGAGCATTCTGTTCCTGGTGTCATCAAGATGTATATTGCTGAAGGAAAGCTTGATCGGGCTAAGTTGTTTTACGAGAAATGCCAGTTGAGTGGTGAGTTATCATCAAAGACATGTGCAGCAATTATAGATGCATATGCTGAAAGGGGATTTTGGACCGAAGCTGAGGCGGTATTCTATAGGAAGAAAGACTTGGTAAGACAAAAGAAGGATGTTATGGAGTATAACGTCATGATCAAAGCTTATGGTAAGGCAAAGCTTTATGATAAAGCTTTTTCACTCTTCAAGGGCATGAGAAATCATGGGACTTGGCCCGATAACTGCACGTATAATTCTCTCATTCAGATGTTCTCAGGAGGTGATTTAGTGGACCAGGCAAGGGATGTTTTAACTGAAATGCGGGAAATGGGTTTTAAGCCACACTCTCTAGCCTTCTCTGCTCTTATTGGATGCTGTGCTCGCCTTGGCCAGCTTTCTGATGCAATTGATGTGTACCAAGACTTGGTCAATTCGGGAGTAAAACCAAATGAATTTGTATATGGTTCTTTGATCAACGGATTTGTAGAAAATGGCAGAGTTGAAGAAGCATTGAAGTATTTTCGTCACATGGAAGAGTCTGGGATTTCTGCAAATCAAATAGTTCTTTCATCCCTCCTAAAGGCATATAGTAAGGTGGACTCCCTTGACGGGGCGAAAGTACTCTATGAGAGATTGAAAGATCTAGAGGGTGCCCGAAATATTGTTGCATCTAACAGCATGATCGATCTTTATGCAGACCATGGGATGGTGTCTGAAGCCGAATTGATTTTTGAAAAACTGAGAGCCAAGGCTTGGGCAAATGAGATAACATATGCGACCATGATATATCTTTACAAGAGTGTGGGCATGCTTGATGAAGCCATTGATATAGCAGAGGAAATGAAACTGTCAGGTCTGGTGAGGGACTGCGGTTCATTTAACAAGGTAATGTCATGTTATGCCATTAACGGGCAGCTCCGTGAATGTGGCGAATTATTGCATGAGATGGTAACTCGGAAACTCTTGCCAGATGTCGGGACTTTTAAAGTATTATTCACAATATTGAAAAAAGGTGTTTCAATTGAAGCAGTGACGCAGTTAGAGTCATCCTACCATGAAGGGAAACCTTACTCTAGGCAAGCTGTCATCACCAGTGTCTTCTCTATGGTGGGTATGCATGCTTTGGCGCTCGAGTCCTGTGAGAAATTCACAAAAACTGATGTAAAGCTCGATTCTTTTCTCTATAACGTCGCGATATATGCTTATGGGGCTGCTGGGGAGATCGACGAGGCTCTGAACATGTTCATGAGAATGCAAGATGAAGACCTGGAGCCAGACGTTGTAACGTATATTAACCTAGTAGGTTGCTATGGAAAAGCTGGTATGGTCGAAGGTGTGAAGCGAATATACAGCCGGTTGAAATACGAAGAGATAGAGCCAAATCATTCCTTGTACAAAGCTGTTATGGATGCCTATACAGATGCCAACAGGCATGACCTTGCCAAACTCGTTAGCCAAGAGATGAGATATGAGTTCTACTCAGAACAAAAGACGGGTTCCGAAACTAAAGATGAGTCCGATGAGGCTACTTCAGATCTTGAGGATAAACAAGTTCATGCATCTAATACCGTATTGTATAAATGTACCGGATTTGACGAAACATGTATTGTATTTCCCATCTGTGTTGTTACACTGTACAGAAACACATTTGCTACTGCAATTTTGTTTTCCAATATAGTAGGGCAAAAAGGGAACCATTCAAATTTCTTTTCCCCTAAACAACAAGATGTGTACAAGTCAATTTTCCCTTCCAATTTTCTCAGCGGCCAAACAAAAGTTAAGGCTCCCTTATTTTTCCTCTGTCAACCGTATTTCCATTTTTATTTTCCACTTGGACGACGGATGGCCGTTGGATTTTCAAAGATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

1101

Amino Acids

123.8

Weight (kDa)

6.9

Isoelectric Point (pI)

29.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_24	PF23276	311 - 410	1e-05	Fungal tetratrico peptide repeats
PPR_2	PF13041	315 - 359	2.8e-12	PPR repeat family
PPR_long	PF17177	315 - 480	4.5e-09	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	315 - 362	3.3e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	316 - 340	1.5e-06	PPR repeat
PPR	PF01535	316 - 345	5.6e-08	PPR repeat
PPR_1	PF12854	344 - 376	1.4e-06	PPR repeat
PPR_2	PF13041	348 - 394	3.4e-12	PPR repeat family
PPR	PF01535	350 - 380	1e-05	PPR repeat
TPR_24	PF23276	358 - 479	9.3e-07	Fungal tetratrico peptide repeats
PPR_3	PF13812	371 - 429	7.9e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	386 - 430	3.2e-07	PPR repeat family
PPR_3	PF13812	521 - 570	1.1e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	523 - 569	2.9e-11	PPR repeat family
TPR_24	PF23276	524 - 619	9.6e-11	Fungal tetratrico peptide repeats
PPR	PF01535	527 - 554	1.4e-06	PPR repeat
PPR_long	PF17177	539 - 646	2e-08	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	557 - 605	9.6e-08	PPR repeat family
PPR_2	PF13041	593 - 640	1.7e-08	PPR repeat family
TPR_24	PF23276	621 - 722	5.1e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	624 - 656	8e-08	PPR repeat
PPR_2	PF13041	630 - 676	1e-07	PPR repeat family
PPR	PF01535	631 - 660	2.3e-07	PPR repeat
PPR_2	PF13041	770 - 812	2e-06	PPR repeat family
PPR_2	PF13041	869 - 911	1.3e-07	PPR repeat family
PPR_3	PF13812	894 - 951	5.8e-06	Pentatricopeptide repeat domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014465)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G73710
fragaria_vesca	FvH4_6g44930
malus_domestica	MD09G1091700.v1.1 MD17G1081600.v1.1
prunus_persica	Prupe.3G233300_v2.0.a1
pyrus_communis	pycom09g01730 pycom17g07990
rosa_chinensis	RchiOBHm_Chr2g0162121
rosa_laevigata	RLG00000021324
rosa_multiflora	Rmu_sc0004020.1_g000031
rosa_roxburghii	Rroxscaffold_2G00088280
rosa_rugosa	Rorug02G0494800
rosa_samantha	Rh2AG561300 Rh2BG574400 Rh2CG544500 Rh2DG584300
rosa_wichuraiana	Rw2G046480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	738, 1161
AccB1I	GGYRCC	1 cut(s)	2084
AccB7I	CCANNNNNTGG	3 cut(s)	1858, 2140, 2187
AccII	CGCG	1 cut(s)	2627
AccIII	TCCGGA	1 cut(s)	661
AciI	CCGC	6 cut(s)	337, 1103, 1526, 1756, 2304, 3198
AcoI	YGGCCR	3 cut(s)	1822, 3199, 3279
AcuI	CTGAAG	3 cut(s)	1419, 2169, 2949
AcvI	CACGTG	1 cut(s)	1282
AcyI	GRCGYC	1 cut(s)	985
AfaI	GTAC	7 cut(s)	971, 1850, 2055, 2828, 3019, 3071, 3167
AflIII	ACRYGT	5 cut(s)	567, 1281, 1469, 2673, 3035
AjnI	CCWGG	3 cut(s)	1372, 1728, 2700
AjuI	GAANNNNNNNTTGG	2 cut(s)	1532, 1564
AleI	CACNNNNGTG	1 cut(s)	625
Alw21I	GWGCWC	5 cut(s)	62, 196, 752, 768, 1816
Alw26I	GTCTC	2 cut(s)	1264, 1310
Alw44I	GTGCAC	1 cut(s)	748
Ama87I	CYCGRG	1 cut(s)	2564
Aor13HI	TCCGGA	1 cut(s)	661
AoxI	GGCC	6 cut(s)	604, 693, 1667, 1822, 3199, 3279
ApaLI	GTGCAC	1 cut(s)	748
ApeKI	GCWGC	5 cut(s)	998, 1205, 1475, 2347, 2645
ArsI	GACNNNNNNTTYG	2 cut(s)	361, 393
Asp700I	GAANNNNTTC	2 cut(s)	445, 2675
AspLEI	GCGC	1 cut(s)	2563
AspS9I	GGNCC	6 cut(s)	693, 1033, 1303, 1512, 1668, 1726
AsuC2I	CCSGG	1 cut(s)	754
AsuHPI	GGTGA	7 cut(s)	78, 398, 806, 1463, 1727, 2305, 2515
AsuII	TTCGAA	2 cut(s)	171, 254
AvaI	CYCGRG	1 cut(s)	2564
AvaII	GGWCC	4 cut(s)	1033, 1303, 1512, 1726
BaeGI	GKGCMC	2 cut(s)	752, 2089
BalI	TGGCCA	1 cut(s)	1824
BanI	GGYRCC	1 cut(s)	2084
BanII	GRGCYC	4 cut(s)	62, 196, 499, 768
BarI	GAAGNNNNNNTAC	2 cut(s)	2765, 2797
BauI	CACGAG	1 cut(s)	189
BbrPI	CACGTG	1 cut(s)	1282
BbsI	GAAGAC	3 cut(s)	318, 2524, 2703
Bbv12I	GWGCWC	5 cut(s)	62, 196, 752, 768, 1816
BbvCI	CCTCAGC	1 cut(s)	1521
BbvI	GCAGC	5 cut(s)	1010, 1192, 1487, 2359, 2632
BccI	CCATC	6 cut(s)	117, 1117, 2134, 2374, 3059, 3271
BceAI	ACGGC	1 cut(s)	3266
BcgI	CGANNNNNNTGC	2 cut(s)	2108, 2142
BciT130I	CCWGG	3 cut(s)	1374, 1730, 2702
BciVI	GTATCC	1 cut(s)	901
BclI	TGATCA	2 cut(s)	1590, 1905
BcnI	CCSGG	1 cut(s)	754
BcoDI	GTCTC	2 cut(s)	1264, 1310
BfaI	CTAG	8 cut(s)	129, 140, 219, 776, 1784, 2078, 2508, 2732
BfmI	CTRYAG	2 cut(s)	342, 1534
BfoI	RGCGCY	1 cut(s)	2564
BfuI	GTATCC	1 cut(s)	901
BglII	AGATCT	2 cut(s)	2074, 2968
BisI	GCNGC	6 cut(s)	999, 1206, 1476, 2348, 2646, 3199
BlpI	GCTNAGC	1 cut(s)	1115
BlsI	GCNGC	6 cut(s)	1000, 1207, 1477, 2349, 2647, 3200
BmcAI	AGTACT	1 cut(s)	2055
Bme1390I	CCNGG	4 cut(s)	754, 1374, 1730, 2702
Bme18I	GGWCC	4 cut(s)	1033, 1303, 1512, 1726
BmeT110I	CYCGRG	1 cut(s)	2564
BmgT120I	GGNCC	6 cut(s)	693, 1033, 1303, 1512, 1668, 1726
BmiI	GGNNCC	7 cut(s)	1034, 1035, 2086, 2706, 2932, 3128, 3219
BmrFI	CCNGG	4 cut(s)	754, 1374, 1730, 2702
BpiI	GAAGAC	3 cut(s)	318, 2524, 2703
BplI	GAGNNNNNCTC	2 cut(s)	2897, 2929
BpmI	CTGGAG	1 cut(s)	2723
Bpu10I	CCTNAGC	1 cut(s)	1521
Bpu1102I	GCTNAGC	1 cut(s)	1115
Bpu14I	TTCGAA	2 cut(s)	171, 254
BpuEI	CTTGAG	2 cut(s)	405, 2993
BpuMI	CCSGG	1 cut(s)	754
Bsa29I	ATCGAT	1 cut(s)	2118
BsaAI	YACGTR	3 cut(s)	570, 1282, 1683
BsaHI	GRCGYC	1 cut(s)	985
BsaJI	CCNNGG	4 cut(s)	30, 1819, 2134, 2181
BsaWI	WCCGGW	2 cut(s)	661, 3020
Bse118I	RCCGGY	2 cut(s)	602, 2787
Bse1I	ACTGG	2 cut(s)	1441, 2526
Bse3DI	GCAATG	1 cut(s)	582
BseAI	TCCGGA	1 cut(s)	661
BseBI	CCWGG	3 cut(s)	1374, 1730, 2702
BseCI	ATCGAT	1 cut(s)	2118
BseDI	CCNNGG	4 cut(s)	30, 1819, 2134, 2181
BseMI	GCAATG	1 cut(s)	582
BseMII	CTCAG	9 cut(s)	209, 512, 638, 831, 1512, 1722, 2165, 2928, 3208
BseNI	ACTGG	2 cut(s)	1441, 2526
BseSI	GKGCMC	2 cut(s)	752, 2089
BseXI	GCAGC	5 cut(s)	1010, 1192, 1487, 2359, 2632
BseYI	CCCAGC	2 cut(s)	436, 2648
BsgI	GTGCAG	2 cut(s)	1494, 1663
Bsh1236I	CGCG	1 cut(s)	2627
BshFI	GGCC	6 cut(s)	606, 695, 1669, 1824, 3201, 3281
BshNI	GGYRCC	1 cut(s)	2084
BshVI	ATCGAT	1 cut(s)	2118
BsiHKAI	GWGCWC	5 cut(s)	62, 196, 752, 768, 1816
BsiHKCI	CYCGRG	1 cut(s)	2564
BsiSI	CCGG	5 cut(s)	603, 662, 753, 2788, 3021
BslFI	GGGAC	4 cut(s)	1019, 1675, 2312, 2425
BsmAI	GTCTC	2 cut(s)	1264, 1310
BsmBI	CGTCTC	1 cut(s)	1264
BsmFI	GGGAC	4 cut(s)	1019, 1675, 2312, 2425
BsmI	GAATGC	2 cut(s)	1363, 2691
BsnI	GGCC	6 cut(s)	606, 695, 1669, 1824, 3201, 3281
BsoBI	CYCGRG	1 cut(s)	2564
Bsp119I	TTCGAA	2 cut(s)	171, 254
Bsp1286I	GDGCHC	7 cut(s)	62, 196, 499, 752, 768, 1816, 2089
Bsp13I	TCCGGA	1 cut(s)	661
Bsp1407I	TGTACA	3 cut(s)	2826, 3069, 3165
Bsp1720I	GCTNAGC	1 cut(s)	1115
Bsp19I	CCATGG	2 cut(s)	30, 2134
Bsp68I	TCGCGA	1 cut(s)	2627
BspACI	CCGC	6 cut(s)	337, 1103, 1526, 1756, 2304, 3198
BspANI	GGCC	6 cut(s)	606, 695, 1669, 1824, 3201, 3281
BspCNI	CTCAG	9 cut(s)	208, 511, 639, 830, 1513, 1721, 2166, 2927, 3207
BspDI	ATCGAT	1 cut(s)	2118
BspEI	TCCGGA	1 cut(s)	661
BspFNI	CGCG	1 cut(s)	2627
BspHI	TCATGA	2 cut(s)	1587, 2679
BspLI	GGNNCC	7 cut(s)	1034, 1035, 2086, 2706, 2932, 3128, 3219
BspT104I	TTCGAA	2 cut(s)	171, 254
BspT107I	GGYRCC	1 cut(s)	2084
BsrDI	GCAATG	1 cut(s)	582
BsrFI	RCCGGY	2 cut(s)	602, 2787
BsrGI	TGTACA	3 cut(s)	2826, 3069, 3165
BsrI	ACTGG	2 cut(s)	1441, 2526
BssAI	RCCGGY	2 cut(s)	602, 2787
BssECI	CCNNGG	4 cut(s)	30, 1819, 2134, 2181
BssNI	GRCGYC	1 cut(s)	985
BssSI	CACGAG	1 cut(s)	189
BssT1I	CCWWGG	4 cut(s)	30, 1819, 2134, 2181
Bst2BI	CACGAG	1 cut(s)	189
Bst2UI	CCWGG	3 cut(s)	1374, 1730, 2702
Bst4CI	ACNGT	9 cut(s)	71, 296, 685, 1217, 1321, 2283, 3004, 3069, 3243
Bst6I	CTCTTC	3 cut(s)	1643, 1962, 2796
BstACI	GRCGYC	1 cut(s)	985
BstAUI	TGTACA	3 cut(s)	2826, 3069, 3165
BstBAI	YACGTR	3 cut(s)	570, 1282, 1683
BstBI	TTCGAA	2 cut(s)	171, 254
BstC8I	GCNNGC	6 cut(s)	604, 1816, 1826, 2243, 2514, 2553
BstDSI	CCRYGG	2 cut(s)	30, 2134
BstENI	CCTNNNNNAGG	2 cut(s)	611, 2012
BstFNI	CGCG	1 cut(s)	2627
BstH2I	RGCGCY	1 cut(s)	2564
BstHHI	GCGC	1 cut(s)	2563
BstMAI	GTCTC	2 cut(s)	1264, 1310
BstMWI	GCNNNNNNNGC	4 cut(s)	1793, 1815, 2633, 2642
BstNI	CCWGG	3 cut(s)	1374, 1730, 2702
BstNSI	RCATGY	5 cut(s)	1473, 2245, 2555, 2677, 3039
BstSCI	CCNGG	4 cut(s)	752, 1372, 1728, 2700
BstSFI	CTRYAG	2 cut(s)	342, 1534
BstSLI	GKGCMC	2 cut(s)	752, 2089
BstUI	CGCG	1 cut(s)	2627
BstV1I	GCAGC	5 cut(s)	1010, 1192, 1487, 2359, 2632
BstV2I	GAAGAC	3 cut(s)	318, 2524, 2703
BstX2I	RGATCY	2 cut(s)	2074, 2968
BstXI	CCANNNNNNTGG	1 cut(s)	1448
BstYI	RGATCY	2 cut(s)	2074, 2968
Bsu15I	ATCGAT	1 cut(s)	2118
BsuI	GTATCC	1 cut(s)	901
BsuRI	GGCC	6 cut(s)	606, 695, 1669, 1824, 3201, 3281
BsuTUI	ATCGAT	1 cut(s)	2118
BtgI	CCRYGG	2 cut(s)	30, 2134
BtsI	GCAGTG	1 cut(s)	2469
BtsIMutI	CAGTG	5 cut(s)	251, 849, 2469, 2533, 3065
BtuMI	TCGCGA	1 cut(s)	2627
Cac8I	GCNNGC	6 cut(s)	604, 1816, 1826, 2243, 2514, 2553
CciI	TCATGA	2 cut(s)	1587, 2679
CfoI	GCGC	1 cut(s)	2563
Cfr10I	RCCGGY	2 cut(s)	602, 2787
Cfr13I	GGNCC	6 cut(s)	693, 1033, 1303, 1512, 1668, 1726
ClaI	ATCGAT	1 cut(s)	2118
CseI	GACGC	2 cut(s)	974, 2476
Csp6I	GTAC	7 cut(s)	970, 1849, 2054, 2827, 3018, 3070, 3166
CspCI	CAANNNNNGTGG	2 cut(s)	850, 885
CviQI	GTAC	7 cut(s)	970, 1849, 2054, 2827, 3018, 3070, 3166
DraI	TTTAAA	1 cut(s)	2419
EaeI	YGGCCR	3 cut(s)	1822, 3199, 3279
Eam1104I	CTCTTC	3 cut(s)	1643, 1962, 2796
EarI	CTCTTC	3 cut(s)	1643, 1962, 2796
Ecl136II	GAGCTC	3 cut(s)	60, 194, 766
Eco130I	CCWWGG	4 cut(s)	30, 1819, 2134, 2181
Eco24I	GRGCYC	4 cut(s)	62, 196, 499, 768
Eco47I	GGWCC	4 cut(s)	1033, 1303, 1512, 1726
Eco53kI	GAGCTC	3 cut(s)	60, 194, 766
Eco57I	CTGAAG	3 cut(s)	1419, 2169, 2949
Eco72I	CACGTG	1 cut(s)	1282
Eco88I	CYCGRG	1 cut(s)	2564
EcoICRI	GAGCTC	3 cut(s)	60, 194, 766
EcoNI	CCTNNNNNAGG	2 cut(s)	611, 2012
EcoO109I	RGGNCCY	1 cut(s)	1033
EcoRI	GAATTC	3 cut(s)	184, 784, 876
EcoRII	CCWGG	3 cut(s)	1372, 1728, 2700
EcoT14I	CCWWGG	4 cut(s)	30, 1819, 2134, 2181
EcoT22I	ATGCAT	3 cut(s)	1492, 2553, 2995
EcoT38I	GRGCYC	4 cut(s)	62, 196, 499, 768
ErhI	CCWWGG	4 cut(s)	30, 1819, 2134, 2181
Esp3I	CGTCTC	1 cut(s)	1264
FaqI	GGGAC	4 cut(s)	1019, 1675, 2312, 2425
FauI	CCCGC	1 cut(s)	1749
FauNDI	CATATG	2 cut(s)	1492, 2206
FbaI	TGATCA	2 cut(s)	1590, 1905
Fnu4HI	GCNGC	6 cut(s)	999, 1206, 1476, 2348, 2646, 3199
FriOI	GRGCYC	4 cut(s)	62, 196, 499, 768
Fsp4HI	GCNGC	6 cut(s)	999, 1206, 1476, 2348, 2646, 3199
FspBI	CTAG	8 cut(s)	129, 140, 219, 776, 1784, 2078, 2508, 2732
GlaI	GCGC	1 cut(s)	2562
GluI	GCNGC	6 cut(s)	999, 1206, 1476, 2348, 2646, 3199
GsaI	CCCAGC	2 cut(s)	440, 2652
GsuI	CTGGAG	1 cut(s)	2723
HaeII	RGCGCY	1 cut(s)	2564
HaeIII	GGCC	6 cut(s)	606, 695, 1669, 1824, 3201, 3281
HapII	CCGG	5 cut(s)	603, 662, 753, 2788, 3021
HgaI	GACGC	2 cut(s)	974, 2476
HhaI	GCGC	1 cut(s)	2563
Hin1I	GRCGYC	1 cut(s)	985
Hin6I	GCGC	1 cut(s)	2561
HinP1I	GCGC	1 cut(s)	2561
HincII	GTYRAC	1 cut(s)	3239
HindII	GTYRAC	1 cut(s)	3239
HindIII	AAGCTT	5 cut(s)	95, 1406, 1596, 1613, 1626
HpaII	CCGG	5 cut(s)	603, 662, 753, 2788, 3021
HphI	GGTGA	7 cut(s)	78, 398, 806, 1463, 1727, 2305, 2515
Hpy166II	GTNNAC	7 cut(s)	750, 849, 1726, 1849, 2032, 3166, 3239
Hpy8I	GTNNAC	7 cut(s)	750, 849, 1726, 1849, 2032, 3166, 3239
Hpy99I	CGWCG	4 cut(s)	78, 2627, 2663, 3276
HpyCH4III	ACNGT	9 cut(s)	71, 296, 685, 1217, 1321, 2283, 3004, 3069, 3243
HpyCH4IV	ACGT	7 cut(s)	569, 1281, 1584, 1682, 2622, 2712, 2720
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1793, 1815, 2633, 2642
HpySE526I	ACGT	7 cut(s)	569, 1281, 1584, 1682, 2622, 2712, 2720
Hsp92I	GRCGYC	1 cut(s)	985
HspAI	GCGC	1 cut(s)	2561
KflI	GGGWCCC	1 cut(s)	1033
Kpn2I	TCCGGA	1 cut(s)	661
KroI	GCCGGC	1 cut(s)	602
KroNI	GCCGGC	1 cut(s)	604
Ksp22I	TGATCA	2 cut(s)	1590, 1905
LmnI	GCTCC	4 cut(s)	32, 2355, 2704, 3223
Lsp1109I	GCAGC	5 cut(s)	1010, 1192, 1487, 2359, 2632
MaeI	CTAG	8 cut(s)	129, 140, 219, 776, 1784, 2078, 2508, 2732
MaeII	ACGT	7 cut(s)	569, 1281, 1584, 1682, 2622, 2712, 2720
MaeIII	GTNAC	9 cut(s)	20, 670, 794, 892, 1958, 2383, 2464, 2716, 3061
MfeI	CAATTG	2 cut(s)	1839, 2454
MflI	RGATCY	2 cut(s)	2074, 2968
MhlI	GDGCHC	7 cut(s)	62, 196, 499, 752, 768, 1816, 2089
MlsI	TGGCCA	1 cut(s)	1824
MluNI	TGGCCA	1 cut(s)	1824
MlyI	GAGTC	7 cut(s)	356, 791, 1979, 2027, 2486, 2576, 2957
MmeI	TCCRAC	3 cut(s)	506, 1077, 3265
Mox20I	TGGCCA	1 cut(s)	1824
Mph1103I	ATGCAT	3 cut(s)	1492, 2553, 2995
MroI	TCCGGA	1 cut(s)	661
MroNI	GCCGGC	1 cut(s)	602
MroXI	GAANNNNTTC	2 cut(s)	445, 2675
MscI	TGGCCA	1 cut(s)	1824
MslI	CAYNNNNRTG	5 cut(s)	625, 1385, 1700, 2678, 2684
Msp20I	TGGCCA	1 cut(s)	1824
MspA1I	CMGCKG	1 cut(s)	3198
MspI	CCGG	5 cut(s)	603, 662, 753, 2788, 3021
MspR9I	CCNGG	4 cut(s)	754, 1374, 1730, 2702
MunI	CAATTG	2 cut(s)	1839, 2454
Mva1269I	GAATGC	2 cut(s)	1363, 2691
MvaI	CCWGG	3 cut(s)	1374, 1730, 2702
MvnI	CGCG	1 cut(s)	2627
MwoI	GCNNNNNNNGC	4 cut(s)	1793, 1815, 2633, 2642
NaeI	GCCGGC	1 cut(s)	604
NciI	CCSGG	1 cut(s)	754
NcoI	CCATGG	2 cut(s)	30, 2134
NdeI	CATATG	2 cut(s)	1492, 2206
NgoMIV	GCCGGC	1 cut(s)	602
NlaIV	GGNNCC	7 cut(s)	1034, 1035, 2086, 2706, 2932, 3128, 3219
NmuCI	GTSAC	3 cut(s)	794, 1958, 2464
NruI	TCGCGA	1 cut(s)	2627
NsiI	ATGCAT	3 cut(s)	1492, 2553, 2995
NspI	RCATGY	5 cut(s)	1473, 2245, 2555, 2677, 3039
NspV	TTCGAA	2 cut(s)	171, 254
OliI	CACNNNNGTG	1 cut(s)	625
PaeI	GCATGC	2 cut(s)	2245, 2555
PaeR7I	CTCGAG	1 cut(s)	2564
PagI	TCATGA	2 cut(s)	1587, 2679
PciI	ACATGT	3 cut(s)	1469, 2673, 3035
PctI	GAATGC	2 cut(s)	1363, 2691
PdiI	GCCGGC	1 cut(s)	604
PdmI	GAANNNNTTC	2 cut(s)	445, 2675
PfeI	GAWTC	4 cut(s)	241, 397, 1324, 2606
PflFI	GACNNNGTC	1 cut(s)	1859
PflMI	CCANNNNNTGG	3 cut(s)	1858, 2140, 2187
PkrI	GCNGC	6 cut(s)	1000, 1207, 1477, 2349, 2647, 3200
PleI	GAGTC	7 cut(s)	355, 791, 1978, 2027, 2485, 2575, 2956
PmaCI	CACGTG	1 cut(s)	1282
PmlI	CACGTG	1 cut(s)	1282
PpsI	GAGTC	7 cut(s)	355, 791, 1978, 2027, 2485, 2575, 2956
Ppu21I	YACGTR	3 cut(s)	570, 1282, 1683
PpuMI	RGGWCCY	1 cut(s)	1033
PscI	ACATGT	3 cut(s)	1469, 2673, 3035
PsiI	TTATAA	2 cut(s)	738, 1161
Psp124BI	GAGCTC	3 cut(s)	62, 196, 768
Psp5II	RGGWCCY	1 cut(s)	1033
Psp6I	CCWGG	3 cut(s)	1372, 1728, 2700
PspCI	CACGTG	1 cut(s)	1282
PspFI	CCCAGC	2 cut(s)	436, 2648
PspGI	CCWGG	3 cut(s)	1372, 1728, 2700
PspN4I	GGNNCC	7 cut(s)	1034, 1035, 2086, 2706, 2932, 3128, 3219
PspPI	GGNCC	6 cut(s)	693, 1033, 1303, 1512, 1668, 1726
PspPPI	RGGWCCY	1 cut(s)	1033
PspXI	VCTCGAGB	1 cut(s)	2564
PsuI	RGATCY	2 cut(s)	2074, 2968
PsyI	GACNNNGTC	1 cut(s)	1859
RruI	TCGCGA	1 cut(s)	2627
RsaI	GTAC	7 cut(s)	971, 1850, 2055, 2828, 3019, 3071, 3167
RsaNI	GTAC	7 cut(s)	970, 1849, 2054, 2827, 3018, 3070, 3166
RseI	CAYNNNNRTG	5 cut(s)	625, 1385, 1700, 2678, 2684
SacI	GAGCTC	3 cut(s)	62, 196, 768
SatI	GCNGC	6 cut(s)	999, 1206, 1476, 2348, 2646, 3199
Sau96I	GGNCC	6 cut(s)	693, 1033, 1303, 1512, 1668, 1726
ScaI	AGTACT	1 cut(s)	2055
SchI	GAGTC	7 cut(s)	356, 791, 1979, 2027, 2486, 2576, 2957
ScrFI	CCNGG	4 cut(s)	754, 1374, 1730, 2702
SduI	GDGCHC	7 cut(s)	62, 196, 499, 752, 768, 1816, 2089
SfcI	CTRYAG	2 cut(s)	342, 1534
Sfr274I	CTCGAG	1 cut(s)	2564
SfuI	TTCGAA	2 cut(s)	171, 254
SinI	GGWCC	4 cut(s)	1033, 1303, 1512, 1726
SlaI	CTCGAG	1 cut(s)	2564
SmiMI	CAYNNNNRTG	5 cut(s)	625, 1385, 1700, 2678, 2684
SmlI	CTYRAG	3 cut(s)	420, 2564, 2972
SmoI	CTYRAG	3 cut(s)	420, 2564, 2972
SphI	GCATGC	2 cut(s)	2245, 2555
SsiI	CCGC	6 cut(s)	337, 1103, 1526, 1756, 2304, 3198
SspI	AATATT	2 cut(s)	2095, 2436
SspMI	CTAG	8 cut(s)	129, 140, 219, 776, 1784, 2078, 2508, 2732
SstI	GAGCTC	3 cut(s)	62, 196, 768
StyD4I	CCNGG	4 cut(s)	752, 1372, 1728, 2700
StyI	CCWWGG	4 cut(s)	30, 1819, 2134, 2181
TaaI	ACNGT	9 cut(s)	71, 296, 685, 1217, 1321, 2283, 3004, 3069, 3243
TaiI	ACGT	7 cut(s)	572, 1284, 1587, 1685, 2625, 2715, 2723
TaqII	GACCGA	1 cut(s)	1529
TatI	WGTACW	4 cut(s)	2053, 2826, 3069, 3165
TauI	GCSGC	1 cut(s)	3201
TfiI	GAWTC	4 cut(s)	241, 397, 1324, 2606
TscAI	CASTG	5 cut(s)	251, 856, 2469, 2533, 3072
TseFI	GTSAC	3 cut(s)	794, 1958, 2464
TseI	GCWGC	5 cut(s)	998, 1205, 1475, 2347, 2645
Tsp45I	GTSAC	3 cut(s)	794, 1958, 2464
TspGWI	ACGGA	3 cut(s)	1926, 2342, 3289
TspRI	CASTG	5 cut(s)	251, 856, 2469, 2533, 3072
Tth111I	GACNNNGTC	1 cut(s)	1859
Van91I	CCANNNNNTGG	3 cut(s)	1858, 2140, 2187
VneI	GTGCAC	1 cut(s)	748
VpaK11BI	GGWCC	4 cut(s)	1033, 1303, 1512, 1726
XagI	CCTNNNNNAGG	2 cut(s)	611, 2012
XbaI	TCTAGA	2 cut(s)	139, 2077
XceI	RCATGY	5 cut(s)	1473, 2245, 2555, 2677, 3039
XhoI	CTCGAG	1 cut(s)	2564
XmnI	GAANNNNTTC	2 cut(s)	445, 2675
XspI	CTAG	8 cut(s)	129, 140, 219, 776, 1784, 2078, 2508, 2732
ZrmI	AGTACT	1 cut(s)	2055
Zsp2I	ATGCAT	3 cut(s)	1492, 2553, 2995

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.