pycom17g13000

Eukaryotic cytochrome b561

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0008301
Physical Location & Seq
Forward (+)
10691806 .. 10695119
3314 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 549 bp
ATGGGGTTTCGGCAGAAATTGGGTTTCTTCTGCATTCGCTTAAGTTTTCTTCTTTTCCTTGTTTCGCCGCTCGTAAGCTCGTCCCAAGAACACATGAAAGGTGCAGGCAGTGACAATGACAACATTCATAAGCAGATGAGTCACAAACTGTCATCGGAAATCAGACTCCACGGGTTTCTTCTTTGGGCTTCAATGGGGTTCTTGATGCCTGTTGGAATACTTGCCATCAGAATGTCACACGGAGAGGAATGTGGAAGAAGGCTCAGAATTCTTTTCTATGTTCATGGCTTATCAGAGATGCTCTCTGTCCTTCTTGCAACAGCAGGAGCAGTGATGTCCTTCAGAAACTTCAACAACTCCTTCAACAATAAACACCAAAGGGTTGGGATAGGCTTATATGGCCTAATATGGTTGCAAGCCCTAATTGGGTTTGTAAGGTCACAGAGGGAATCCAAGGGAAGAAGCATATGGTTTTCTGTACATTGGATAATTGGAACTGTCTCTGCTTGGGGTTCTCAACATATACACAGGACTACAAGCCTACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

183

Amino Acids

20.59

Weight (kDa)

10.89

Isoelectric Point (pI)

59.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 56 - 165 3.7e-06 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 70
AciI CCGC 1 cut(s) 68
AcsI RAATTY 1 cut(s) 267
AcuI CTGAAG 1 cut(s) 325
AfaI GTAC 1 cut(s) 480
AfiI CCNNNNNNNGG 1 cut(s) 426
AflII CTTAAG 1 cut(s) 40
AgsI TTSAA 3 cut(s) 192, 352, 364
AluBI AGCT 1 cut(s) 78
AluI AGCT 1 cut(s) 78
Alw26I GTCTC 1 cut(s) 505
AoxI GGCC 1 cut(s) 400
ApoI RAATTY 1 cut(s) 267
BccI CCATC 1 cut(s) 233
BcoDI GTCTC 1 cut(s) 505
BfrI CTTAAG 1 cut(s) 40
BisI GCNGC 1 cut(s) 68
BlsI GCNGC 1 cut(s) 69
BmsI GCATC 2 cut(s) 195, 288
BplI GAGNNNNNCTC 2 cut(s) 287, 319
BsaJI CCNNGG 2 cut(s) 169, 453
Bsc4I CCNNNNNNNGG 1 cut(s) 426
BseDI CCNNGG 2 cut(s) 169, 453
BseLI CCNNNNNNNGG 1 cut(s) 426
BseMII CTCAG 1 cut(s) 277
BsgI GTGCAG 1 cut(s) 123
BshFI GGCC 1 cut(s) 402
BslFI GGGAC 1 cut(s) 67
BslI CCNNNNNNNGG 1 cut(s) 426
BsmAI GTCTC 1 cut(s) 505
BsmFI GGGAC 1 cut(s) 67
BsmI GAATGC 1 cut(s) 33
BsnI GGCC 1 cut(s) 402
Bsp1407I TGTACA 1 cut(s) 478
BspACI CCGC 1 cut(s) 68
BspANI GGCC 1 cut(s) 402
BspCNI CTCAG 1 cut(s) 276
BspTI CTTAAG 1 cut(s) 40
BsrBI CCGCTC 1 cut(s) 70
BsrGI TGTACA 1 cut(s) 478
BssECI CCNNGG 2 cut(s) 169, 453
BssT1I CCWWGG 1 cut(s) 453
Bst4CI ACNGT 2 cut(s) 150, 499
BstAFI CTTAAG 1 cut(s) 40
BstAUI TGTACA 1 cut(s) 478
BstC8I GCNNGC 2 cut(s) 106, 417
BstDEI CTNAG 1 cut(s) 263
BstDSI CCRYGG 1 cut(s) 169
BstMAI GTCTC 1 cut(s) 505
BstMWI GCNNNNNNNGC 1 cut(s) 399
BstXI CCANNNNNNTGG 1 cut(s) 383
BsuRI GGCC 1 cut(s) 402
BtgI CCRYGG 1 cut(s) 169
BtsI GCAGTG 2 cut(s) 115, 336
BtsIMutI CAGTG 2 cut(s) 115, 336
Cac8I GCNNGC 2 cut(s) 106, 417
Csp6I GTAC 1 cut(s) 479
CviAII CATG 3 cut(s) 94, 284, 546
CviJI RGCY 8 cut(s) 78, 188, 262, 288, 393, 402, 419, 540
CviKI_1 RGCY 8 cut(s) 78, 188, 262, 288, 393, 402, 419, 540
CviQI GTAC 1 cut(s) 479
DdeI CTNAG 1 cut(s) 263
Eco130I CCWWGG 1 cut(s) 453
Eco57I CTGAAG 1 cut(s) 325
EcoRI GAATTC 1 cut(s) 267
EcoT14I CCWWGG 1 cut(s) 453
ErhI CCWWGG 1 cut(s) 453
FaeI CATG 3 cut(s) 97, 287, 549
FaqI GGGAC 1 cut(s) 67
FatI CATG 3 cut(s) 93, 283, 545
FauNDI CATATG 1 cut(s) 467
Fnu4HI GCNGC 1 cut(s) 68
Fsp4HI GCNGC 1 cut(s) 68
GluI GCNGC 1 cut(s) 68
HaeIII GGCC 1 cut(s) 402
Hin1II CATG 3 cut(s) 97, 287, 549
HinfI GANTC 3 cut(s) 139, 165, 449
Hpy188I TCNGA 6 cut(s) 157, 164, 230, 266, 295, 344
Hpy188III TCNNGA 1 cut(s) 202
HpyAV CCTTC 4 cut(s) 252, 320, 349, 370
HpyCH4III ACNGT 2 cut(s) 150, 499
HpyCH4V TGCA 4 cut(s) 33, 104, 317, 415
HpyF10VI GCNNNNNNNGC 1 cut(s) 399
HpyF3I CTNAG 1 cut(s) 263
Hsp92II CATG 3 cut(s) 97, 287, 549
LmnI GCTCC 1 cut(s) 326
LpnPI CCDG 4 cut(s) 90, 222, 309, 514
LweI GCATC 2 cut(s) 195, 288
MaeIII GTNAC 4 cut(s) 110, 140, 234, 438
MbiI CCGCTC 1 cut(s) 70
MboII GAAGA 5 cut(s) 19, 41, 170, 267, 471
MluCI AATT 4 cut(s) 17, 267, 423, 489
MlyI GAGTC 2 cut(s) 148, 159
MmeI TCCRAC 1 cut(s) 193
MnlI CCTC 2 cut(s) 238, 438
MseI TTAA 1 cut(s) 41
MslI CAYNNNNRTG 1 cut(s) 230
MspCI CTTAAG 1 cut(s) 40
Mva1269I GAATGC 1 cut(s) 33
MwoI GCNNNNNNNGC 1 cut(s) 399
NdeI CATATG 1 cut(s) 467
NlaIII CATG 3 cut(s) 97, 287, 549
NmuCI GTSAC 4 cut(s) 110, 140, 234, 438
PctI GAATGC 1 cut(s) 33
PfeI GAWTC 1 cut(s) 449
PkrI GCNGC 1 cut(s) 69
PleI GAGTC 2 cut(s) 147, 159
PpsI GAGTC 2 cut(s) 147, 159
RsaI GTAC 1 cut(s) 480
RsaNI GTAC 1 cut(s) 479
RseI CAYNNNNRTG 1 cut(s) 230
SaqAI TTAA 1 cut(s) 41
SatI GCNGC 1 cut(s) 68
SchI GAGTC 2 cut(s) 148, 159
SetI ASST 3 cut(s) 80, 103, 440
SfaNI GCATC 2 cut(s) 195, 288
SmiMI CAYNNNNRTG 1 cut(s) 230
SmlI CTYRAG 1 cut(s) 40
SmoI CTYRAG 1 cut(s) 40
Sse9I AATT 4 cut(s) 17, 267, 423, 489
SsiI CCGC 1 cut(s) 68
StyI CCWWGG 1 cut(s) 453
TaaI ACNGT 2 cut(s) 150, 499
TasI AATT 4 cut(s) 17, 267, 423, 489
TatI WGTACW 1 cut(s) 478
TauI GCSGC 1 cut(s) 70
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 1 cut(s) 41
Tru9I TTAA 1 cut(s) 41
TscAI CASTG 2 cut(s) 115, 336
TseFI GTSAC 4 cut(s) 110, 140, 234, 438
Tsp45I GTSAC 4 cut(s) 110, 140, 234, 438
TspDTI ATGAA 3 cut(s) 110, 116, 272
TspGWI ACGGA 1 cut(s) 255
TspRI CASTG 2 cut(s) 115, 336
Vha464I CTTAAG 1 cut(s) 40
XapI RAATTY 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.