pycom17g14570

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0008519
Physical Location & Seq
Reverse (-)
12132993 .. 12135160
2168 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1161 bp
ATGTGTGGAGGTGCTATTATTTCTGATTTCATAGCGCCGCCGGCGCGGTCCCGGCGGCTCACCACCGACTGCCTCTGGCCCGATCTCAAAAAACCCAGTTCGGGGAAGCGGTTCTCGAAGCCTCTGAGGTCCGTAATCGTTGACTTGGACGACGACTTCGAGGCTGATTTCCAGGAATTCAAGGACGAGTCCGATGTGGACGAGGACGATGAAATGGTTGATTCCAAGCCCTCTGCTTTCTCTGCCGGAAAGCCCTCTTCTGCCCGTGGTTCTGCTGTGAAATCTGTGGAGTTCAATGGGCAAGCTGAGAAATCTGCAAAGAGAAAAAGGAAGAACCAGTACAGGGGAATTCGCCAGCGCCCATGGGGAAAGTGGGCTGCAGAGATCCGCGACCCAAGGAAAGGGGTTCGGGTTTGGCTTGGAACTTTCAACACTGCAGAAGAAGCTGCAAGGGCATATGACGCCGAGGCACGCAGAATTCGTGGTAAGAAAGCTAAGGTTAACTTCCCTGAAGAAACCCCTCGTGCTTCTGCAAAGCGTTCTGTGAAGGCAAATTCTCAGAAACTGATACCCAAGACAAACGTGAATGGCACTGAGTCTAGTCTGAACCAGAATTTCAATTTTGTGAACGACTCAAGTGAGGACTATTACGGTGCTCTGGGTTTTCTGGATGAAAAGCCAACGTTGAATAACTTTGGGTATATGTCTACCTTCCCTGCCAATGGAGATGTTGCACTGAAATCCTCTACTCCATCCGATCCTGCCCCCTTTTATTTGAGTTCCGATCAGGGAAGCAACTCGTTTGATTGTTCTGAGTTCGGCTGGGGAGAACAAGGCTCAAAGACTCCAGAAATCTCATCAGTTCTTTCTTCTGTGATGGAAGAAACTGACGACTCACTGTTTCTGGAGGATGCTAACCCAACGAAGAAGCTGAAGCCCAACTCACGGGATCTGGTGCTTCCTCAGGATAGTACAGGAAAGACACTGTCTGATGAGCTCTCAGCTTTTGAGATGAAGTACTTTCAGACCCCATATCTTGACGGGAGCTGGGATGCTTCAGTGGACGCCTTCCTTAACGGAGACGCAACTCAGGATGGTGGTAATCCAGTGGACCTATGGACCTTTGACGATCTGCCGGAAATTGTTGGAGGAACTTTCTGA

Protein Analysis

387

Amino Acids

42.62

Weight (kDa)

4.86

Isoelectric Point (pI)

39.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 113 - 162 3.7e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 707
AccII CGCG 2 cut(s) 46, 390
AciI CCGC 5 cut(s) 38, 46, 55, 109, 388
AclI AACGTT 1 cut(s) 683
AclWI GGATC 3 cut(s) 379, 752, 957
AcsI RAATTY 5 cut(s) 176, 348, 477, 553, 613
AcuI CTGAAG 3 cut(s) 531, 953, 1041
AcyI GRCGYC 2 cut(s) 462, 1065
AfaI GTAC 3 cut(s) 341, 973, 1019
AfiI CCNNNNNNNGG 6 cut(s) 101, 102, 343, 401, 722, 945
AgsI TTSAA 5 cut(s) 181, 295, 430, 619, 688
AjnI CCWGG 1 cut(s) 171
AluBI AGCT 7 cut(s) 305, 446, 494, 931, 997, 1004, 1047
AluI AGCT 7 cut(s) 305, 446, 494, 931, 997, 1004, 1047
Alw21I GWGCWC 2 cut(s) 658, 999
Alw26I GTCTC 1 cut(s) 1074
AlwI GGATC 3 cut(s) 379, 752, 957
AlwNI CAGNNNCTG 1 cut(s) 565
AoxI GGCC 1 cut(s) 77
ApeKI GCWGC 2 cut(s) 377, 446
ApoI RAATTY 5 cut(s) 176, 348, 477, 553, 613
ArsI GACNNNNNNTTYG 2 cut(s) 140, 172
Asp700I GAANNNNTTC 1 cut(s) 110
AspLEI GCGC 3 cut(s) 37, 46, 360
AspS9I GGNCC 5 cut(s) 48, 78, 129, 1111, 1119
AsuC2I CCSGG 1 cut(s) 52
AsuHPI GGTGA 1 cut(s) 52
AvaII GGWCC 4 cut(s) 48, 129, 1111, 1119
AxyI CCTNAGG 1 cut(s) 963
BanII GRGCYC 1 cut(s) 999
BarI GAAGNNNNNNTAC 2 cut(s) 323, 355
BauI CACGAG 1 cut(s) 522
Bbv12I GWGCWC 2 cut(s) 658, 999
BbvI GCAGC 2 cut(s) 364, 433
BccI CCATC 3 cut(s) 760, 871, 1088
BciT130I CCWGG 1 cut(s) 173
BcnI CCSGG 1 cut(s) 52
BcoDI GTCTC 1 cut(s) 1074
BfaI CTAG 1 cut(s) 600
BfmI CTRYAG 2 cut(s) 378, 435
BfoI RGCGCY 2 cut(s) 38, 361
BisI GCNGC 4 cut(s) 38, 56, 378, 447
BlsI GCNGC 4 cut(s) 39, 57, 379, 448
BmcAI AGTACT 1 cut(s) 1019
Bme1390I CCNGG 2 cut(s) 52, 173
Bme18I GGWCC 4 cut(s) 48, 129, 1111, 1119
BmgT120I GGNCC 5 cut(s) 48, 78, 129, 1111, 1119
BmiI GGNNCC 1 cut(s) 50
BmrFI CCNGG 2 cut(s) 52, 173
BmrI ACTGGG 1 cut(s) 90
BmsI GCATC 2 cut(s) 901, 1042
BmuI ACTGGG 1 cut(s) 90
BpmI CTGGAG 2 cut(s) 831, 926
Bpu10I CCTNAGC 1 cut(s) 495
BpuEI CTTGAG 1 cut(s) 619
BpuMI CCSGG 1 cut(s) 52
BsaHI GRCGYC 2 cut(s) 462, 1065
BsaJI CCNNGG 4 cut(s) 265, 362, 395, 465
Bsc4I CCNNNNNNNGG 6 cut(s) 101, 102, 343, 401, 722, 945
Bse118I RCCGGY 1 cut(s) 40
Bse1I ACTGG 3 cut(s) 96, 337, 1106
Bse21I CCTNAGG 1 cut(s) 963
BseBI CCWGG 1 cut(s) 173
BseDI CCNNGG 4 cut(s) 265, 362, 395, 465
BseGI GGATG 5 cut(s) 676, 752, 916, 1057, 1099
BseLI CCNNNNNNNGG 6 cut(s) 101, 102, 343, 401, 722, 945
BseMII CTCAG 8 cut(s) 116, 297, 572, 585, 804, 977, 1014, 1103
BseNI ACTGG 3 cut(s) 96, 337, 1106
BseXI GCAGC 2 cut(s) 364, 433
BseYI CCCAGC 2 cut(s) 822, 1047
Bsh1236I CGCG 2 cut(s) 46, 390
BshFI GGCC 1 cut(s) 79
BsiHKAI GWGCWC 2 cut(s) 658, 999
BsiSI CCGG 4 cut(s) 41, 52, 246, 1136
BslFI GGGAC 1 cut(s) 34
BslI CCNNNNNNNGG 6 cut(s) 101, 102, 343, 401, 722, 945
BsmAI GTCTC 1 cut(s) 1074
BsmBI CGTCTC 1 cut(s) 1074
BsmFI GGGAC 1 cut(s) 34
BsnI GGCC 1 cut(s) 79
Bsp1286I GDGCHC 2 cut(s) 658, 999
Bsp143I GATC 6 cut(s) 82, 384, 757, 784, 949, 1129
Bsp19I CCATGG 1 cut(s) 362
BspACI CCGC 5 cut(s) 38, 46, 55, 109, 388
BspANI GGCC 1 cut(s) 79
BspCNI CTCAG 8 cut(s) 117, 298, 571, 586, 805, 976, 1013, 1102
BspFNI CGCG 2 cut(s) 46, 390
BspLI GGNNCC 1 cut(s) 50
BspMAI CTGCAG 2 cut(s) 382, 439
BspPI GGATC 3 cut(s) 379, 752, 957
BsrFI RCCGGY 1 cut(s) 40
BsrI ACTGG 3 cut(s) 96, 337, 1106
BssAI RCCGGY 1 cut(s) 40
BssECI CCNNGG 4 cut(s) 265, 362, 395, 465
BssMI GATC 6 cut(s) 82, 384, 757, 784, 949, 1129
BssNI GRCGYC 2 cut(s) 462, 1065
BssSI CACGAG 1 cut(s) 522
BssT1I CCWWGG 2 cut(s) 362, 395
Bst2BI CACGAG 1 cut(s) 522
Bst2UI CCWGG 1 cut(s) 173
Bst4CI ACNGT 3 cut(s) 653, 900, 987
Bst6I CTCTTC 1 cut(s) 262
BstACI GRCGYC 2 cut(s) 462, 1065
BstC8I GCNNGC 4 cut(s) 42, 303, 356, 472
BstDEI CTNAG 9 cut(s) 125, 306, 495, 558, 594, 813, 963, 1000, 1089
BstDSI CCRYGG 2 cut(s) 265, 362
BstF5I GGATG 5 cut(s) 676, 752, 916, 1057, 1099
BstFNI CGCG 2 cut(s) 46, 390
BstH2I RGCGCY 2 cut(s) 38, 361
BstHHI GCGC 3 cut(s) 37, 46, 360
BstKTI GATC 6 cut(s) 85, 387, 760, 787, 952, 1132
BstMAI GTCTC 1 cut(s) 1074
BstMBI GATC 6 cut(s) 82, 384, 757, 784, 949, 1129
BstMWI GCNNNNNNNGC 7 cut(s) 41, 43, 52, 242, 443, 452, 461
BstNI CCWGG 1 cut(s) 173
BstSCI CCNGG 2 cut(s) 50, 171
BstSFI CTRYAG 2 cut(s) 378, 435
BstUI CGCG 2 cut(s) 46, 390
BstV1I GCAGC 2 cut(s) 364, 433
BstX2I RGATCY 2 cut(s) 384, 949
BstYI RGATCY 2 cut(s) 384, 949
Bsu36I CCTNAGG 1 cut(s) 963
BsuRI GGCC 1 cut(s) 79
BtgI CCRYGG 2 cut(s) 265, 362
BtsCI GGATG 5 cut(s) 676, 752, 916, 1057, 1099
BtsI GCAGTG 1 cut(s) 432
BtsIMutI CAGTG 7 cut(s) 432, 591, 734, 896, 983, 1065, 1113
Cac8I GCNNGC 4 cut(s) 42, 303, 356, 472
CaiI CAGNNNCTG 1 cut(s) 565
CfoI GCGC 3 cut(s) 37, 46, 360
Cfr10I RCCGGY 1 cut(s) 40
Cfr13I GGNCC 5 cut(s) 48, 78, 129, 1111, 1119
CseI GACGC 3 cut(s) 470, 1073, 1091
Csp6I GTAC 3 cut(s) 340, 972, 1018
CviAII CATG 1 cut(s) 363
CviQI GTAC 3 cut(s) 340, 972, 1018
DdeI CTNAG 9 cut(s) 125, 306, 495, 558, 594, 813, 963, 1000, 1089
DpnI GATC 6 cut(s) 84, 386, 759, 786, 951, 1131
DpnII GATC 6 cut(s) 82, 384, 757, 784, 949, 1129
Eam1104I CTCTTC 1 cut(s) 262
EarI CTCTTC 1 cut(s) 262
Ecl136II GAGCTC 1 cut(s) 997
Eco130I CCWWGG 2 cut(s) 362, 395
Eco24I GRGCYC 1 cut(s) 999
Eco47I GGWCC 4 cut(s) 48, 129, 1111, 1119
Eco53kI GAGCTC 1 cut(s) 997
Eco57I CTGAAG 3 cut(s) 531, 953, 1041
Eco81I CCTNAGG 1 cut(s) 963
EcoICRI GAGCTC 1 cut(s) 997
EcoRI GAATTC 3 cut(s) 176, 348, 477
EcoRII CCWGG 1 cut(s) 171
EcoT14I CCWWGG 2 cut(s) 362, 395
EcoT38I GRGCYC 1 cut(s) 999
ErhI CCWWGG 2 cut(s) 362, 395
Esp3I CGTCTC 1 cut(s) 1074
FaeI CATG 1 cut(s) 366
FaiI YATR 8 cut(s) 32, 364, 457, 459, 702, 704, 1033, 1117
FalI AAGNNNNNCTT 2 cut(s) 488, 520
FaqI GGGAC 1 cut(s) 34
FatI CATG 1 cut(s) 362
FauNDI CATATG 1 cut(s) 457
FblI GTMKAC 1 cut(s) 707
Fnu4HI GCNGC 4 cut(s) 38, 56, 378, 447
FokI GGATG 5 cut(s) 683, 739, 923, 1064, 1106
FriOI GRGCYC 1 cut(s) 999
Fsp4HI GCNGC 4 cut(s) 38, 56, 378, 447
FspBI CTAG 1 cut(s) 600
GlaI GCGC 3 cut(s) 36, 45, 359
GluI GCNGC 4 cut(s) 38, 56, 378, 447
GsaI CCCAGC 2 cut(s) 826, 1051
GsuI CTGGAG 2 cut(s) 831, 926
HaeII RGCGCY 2 cut(s) 38, 361
HaeIII GGCC 1 cut(s) 79
HapII CCGG 4 cut(s) 41, 52, 246, 1136
HgaI GACGC 3 cut(s) 470, 1073, 1091
HhaI GCGC 3 cut(s) 37, 46, 360
Hin1I GRCGYC 2 cut(s) 462, 1065
Hin1II CATG 1 cut(s) 366
Hin6I GCGC 3 cut(s) 35, 44, 358
HinP1I GCGC 3 cut(s) 35, 44, 358
HincII GTYRAC 2 cut(s) 142, 502
HindII GTYRAC 2 cut(s) 142, 502
HinfI GANTC 6 cut(s) 188, 221, 596, 632, 844, 893
HpaI GTTAAC 1 cut(s) 502
HpaII CCGG 4 cut(s) 41, 52, 246, 1136
HphI GGTGA 1 cut(s) 52
Hpy166II GTNNAC 7 cut(s) 142, 199, 502, 628, 708, 1063, 1111
Hpy188III TCNNGA 7 cut(s) 115, 668, 848, 905, 965, 1037, 1091
Hpy8I GTNNAC 7 cut(s) 142, 199, 502, 628, 708, 1063, 1111
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 3 cut(s) 541, 721, 1078
HpyCH4III ACNGT 3 cut(s) 653, 900, 987
HpyCH4IV ACGT 2 cut(s) 582, 683
HpyCH4V TGCA 6 cut(s) 317, 380, 437, 449, 533, 734
HpyF10VI GCNNNNNNNGC 7 cut(s) 41, 43, 52, 242, 443, 452, 461
HpyF3I CTNAG 9 cut(s) 125, 306, 495, 558, 594, 813, 963, 1000, 1089
HpySE526I ACGT 2 cut(s) 582, 683
Hsp92I GRCGYC 2 cut(s) 462, 1065
Hsp92II CATG 1 cut(s) 366
HspAI GCGC 3 cut(s) 35, 44, 358
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
KspAI GTTAAC 1 cut(s) 502
Kzo9I GATC 6 cut(s) 82, 384, 757, 784, 949, 1129
LmnI GCTCC 1 cut(s) 1044
Lsp1109I GCAGC 2 cut(s) 364, 433
LweI GCATC 2 cut(s) 901, 1042
MaeI CTAG 1 cut(s) 600
MaeII ACGT 2 cut(s) 582, 683
MalI GATC 6 cut(s) 84, 386, 759, 786, 951, 1131
MboI GATC 6 cut(s) 82, 384, 757, 784, 949, 1129
MboII GAAGA 7 cut(s) 249, 343, 452, 524, 861, 893, 937
MflI RGATCY 2 cut(s) 384, 949
MhlI GDGCHC 2 cut(s) 658, 999
MluCI AATT 7 cut(s) 176, 348, 477, 553, 613, 619, 1140
MlyI GAGTC 5 cut(s) 197, 605, 626, 838, 887
MmeI TCCRAC 1 cut(s) 1126
MreI CGCCGGCG 1 cut(s) 40
MroNI GCCGGC 1 cut(s) 40
MroXI GAANNNNTTC 1 cut(s) 110
MseI TTAA 2 cut(s) 501, 1074
MspI CCGG 4 cut(s) 41, 52, 246, 1136
MspR9I CCNGG 2 cut(s) 52, 173
MvaI CCWGG 1 cut(s) 173
MvnI CGCG 2 cut(s) 46, 390
MwoI GCNNNNNNNGC 7 cut(s) 41, 43, 52, 242, 443, 452, 461
NaeI GCCGGC 1 cut(s) 42
NciI CCSGG 1 cut(s) 52
NcoI CCATGG 1 cut(s) 362
NdeI CATATG 1 cut(s) 457
NdeII GATC 6 cut(s) 82, 384, 757, 784, 949, 1129
NgoMIV GCCGGC 1 cut(s) 40
NlaIII CATG 1 cut(s) 366
NlaIV GGNNCC 1 cut(s) 50
NmeAIII GCCGAG 1 cut(s) 490
PcsI WCGNNNNNNNCGW 2 cut(s) 156, 478
PdiI GCCGGC 1 cut(s) 42
PdmI GAANNNNTTC 1 cut(s) 110
PfeI GAWTC 1 cut(s) 221
PflFI GACNNNGTC 1 cut(s) 985
PfoI TCCNGGA 1 cut(s) 171
PkrI GCNGC 4 cut(s) 39, 57, 379, 448
PleI GAGTC 5 cut(s) 196, 604, 626, 838, 887
PpsI GAGTC 5 cut(s) 196, 604, 626, 838, 887
Psp124BI GAGCTC 1 cut(s) 999
Psp1406I AACGTT 1 cut(s) 683
Psp6I CCWGG 1 cut(s) 171
PspFI CCCAGC 2 cut(s) 822, 1047
PspGI CCWGG 1 cut(s) 171
PspN4I GGNNCC 1 cut(s) 50
PspPI GGNCC 5 cut(s) 48, 78, 129, 1111, 1119
PstI CTGCAG 2 cut(s) 382, 439
PstNI CAGNNNCTG 1 cut(s) 565
PsuI RGATCY 2 cut(s) 384, 949
PsyI GACNNNGTC 1 cut(s) 985
RsaI GTAC 3 cut(s) 341, 973, 1019
RsaNI GTAC 3 cut(s) 340, 972, 1018
SacI GAGCTC 1 cut(s) 999
SaqAI TTAA 2 cut(s) 501, 1074
SatI GCNGC 4 cut(s) 38, 56, 378, 447
Sau3AI GATC 6 cut(s) 82, 384, 757, 784, 949, 1129
Sau96I GGNCC 5 cut(s) 48, 78, 129, 1111, 1119
ScaI AGTACT 1 cut(s) 1019
SchI GAGTC 5 cut(s) 197, 605, 626, 838, 887
ScrFI CCNGG 2 cut(s) 52, 173
SduI GDGCHC 2 cut(s) 658, 999
SfaNI GCATC 2 cut(s) 901, 1042
SfcI CTRYAG 2 cut(s) 378, 435
SgrAI CRCCGGYG 1 cut(s) 40
SinI GGWCC 4 cut(s) 48, 129, 1111, 1119
SmlI CTYRAG 1 cut(s) 634
SmoI CTYRAG 1 cut(s) 634
Sse9I AATT 7 cut(s) 176, 348, 477, 553, 613, 619, 1140
SsiI CCGC 5 cut(s) 38, 46, 55, 109, 388
SspMI CTAG 1 cut(s) 600
SstI GAGCTC 1 cut(s) 999
StyD4I CCNGG 2 cut(s) 50, 171
StyI CCWWGG 2 cut(s) 362, 395
TaaI ACNGT 3 cut(s) 653, 900, 987
TaiI ACGT 2 cut(s) 585, 686
TaqI TCGA 2 cut(s) 116, 159
TasI AATT 7 cut(s) 176, 348, 477, 553, 613, 619, 1140
TatI WGTACW 3 cut(s) 339, 971, 1017
TauI GCSGC 2 cut(s) 40, 58
TfiI GAWTC 1 cut(s) 221
Tru1I TTAA 2 cut(s) 501, 1074
Tru9I TTAA 2 cut(s) 501, 1074
TscAI CASTG 7 cut(s) 439, 598, 741, 903, 990, 1065, 1113
TseI GCWGC 2 cut(s) 377, 446
TspDTI ATGAA 4 cut(s) 19, 225, 687, 1028
TspGWI ACGGA 2 cut(s) 121, 1092
TspRI CASTG 7 cut(s) 439, 598, 741, 903, 990, 1065, 1113
Tth111I GACNNNGTC 1 cut(s) 985
VpaK11BI GGWCC 4 cut(s) 48, 129, 1111, 1119
XapI RAATTY 5 cut(s) 176, 348, 477, 553, 613
XcmI CCANNNNNNNNNTGG 2 cut(s) 369, 1113
XmiI GTMKAC 1 cut(s) 707
XmnI GAANNNNTTC 1 cut(s) 110
XspI CTAG 1 cut(s) 600
ZrmI AGTACT 1 cut(s) 1019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.