pycom17g19830

Photosystem I reaction center subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0013380
Physical Location & Seq
Forward (+)
17865672 .. 17867179
1508 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 621 bp
ATGGATTCATATCCTCTAAACAAAACTCATCTCGCCACGTGTCAAGCTAAGATCCATGCAAAAGATCAAAAACCCAATCACGCTGAGTTCCCCACGCCCCTTAATGCAACCGAGAAACAGAAGAGAAAAAGAAAGGTAAGTCCAGATACTGAAAGAGTAGCAGTAGACCAGTATTATTGTAAGGCCATGGCATCTCTGGCAACCCTAGCAGCCGTTCAACCGGTCACCATTAACGGCCTCGGCGGAAGCTCCCTCACCGGAACAAAGCTCGTTGTGAAGCCCACTCGCCAGAGCATCAGAACAAAAAACATCAGGAGTGGTGCTGTGGTGGCCAAGTACGGAGACAAGAGCGTCTACTTTGACCTTGAGGATCTGGGCAACACCACCGGGAAGTGGGACTTGTACGGTTCAGATGCCCCCTCACCTTACAACCCTCTTCAGAGCAAGTTCTTTGAGACATTTGCCGCTCCATTCACCAAGAGAGGCTTACTCCTCAAGTTCTTGATATTGGGAGGAGCTTCCACCATTGCCTACCTTAGTGCCACTGCCTCCGACGACGTTCTGCCAATCAAGAAGGGCCCACAACTTCCACCCAAGTTGGGCCCACGTGGCAAGATCTAA

Protein Analysis

207

Amino Acids

22.37

Weight (kDa)

9.78

Isoelectric Point (pI)

38.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSI_PsaH PF03244 69 - 206 3.3e-71 Photosystem I reaction centre subunit VI
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013380)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52230 AT3G16140
fragaria_vesca FvH4_6g31740
malus_domestica MD09G1209500.v1.1
prunus_persica Prupe.3G067400_v2.0.a1
pyrus_communis pycom09g12570 pycom17g19830
rosa_chinensis RchiOBHm_Chr2g0138931
rosa_laevigata RLG00000019769
rosa_multiflora Rmu_sc0001376.1_g000002
rosa_roxburghii Rroxscaffold_2G00105860
rosa_rugosa Rorug02G0353200 Rorug02G0353300
rosa_samantha Rh2AG403300 Rh2BG413800 Rh2CG389400 Rh2DG423600
rosa_wichuraiana Rw2G032810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 350
AccBSI CCGCTC 1 cut(s) 467
AccI GTMKAC 2 cut(s) 165, 354
AciI CCGC 2 cut(s) 243, 465
AclWI GGATC 2 cut(s) 46, 378
AcoI YGGCCR 1 cut(s) 330
AcuI CTGAAG 1 cut(s) 422
AcvI CACGTG 2 cut(s) 39, 608
AfaI GTAC 2 cut(s) 338, 404
AfiI CCNNNNNNNGG 2 cut(s) 393, 599
AflIII ACRYGT 1 cut(s) 38
AgeI ACCGGT 1 cut(s) 220
AgsI TTSAA 1 cut(s) 218
AluBI AGCT 4 cut(s) 47, 249, 268, 518
AluI AGCT 4 cut(s) 47, 249, 268, 518
Alw26I GTCTC 2 cut(s) 336, 449
AlwI GGATC 2 cut(s) 46, 378
AlwNI CAGNNNCTG 1 cut(s) 149
AoxI GGCC 5 cut(s) 183, 235, 330, 577, 601
ApaI GGGCCC 2 cut(s) 581, 605
ApeKI GCWGC 1 cut(s) 209
AsiGI ACCGGT 1 cut(s) 220
AspS9I GGNCC 4 cut(s) 577, 578, 601, 602
AsuC2I CCSGG 1 cut(s) 388
AsuHPI GGTGA 4 cut(s) 217, 247, 414, 466
BaeGI GKGCMC 2 cut(s) 581, 605
BalI TGGCCA 1 cut(s) 332
BanII GRGCYC 2 cut(s) 581, 605
BbrPI CACGTG 2 cut(s) 39, 608
BbvI GCAGC 1 cut(s) 221
BceAI ACGGC 2 cut(s) 197, 250
BcnI CCSGG 1 cut(s) 388
BcoDI GTCTC 2 cut(s) 336, 449
BfaI CTAG 1 cut(s) 206
BglI GCCNNNNNGGC 1 cut(s) 609
BglII AGATCT 1 cut(s) 615
BisI GCNGC 2 cut(s) 210, 465
BlsI GCNGC 2 cut(s) 211, 466
Bme1390I CCNGG 1 cut(s) 388
BmgT120I GGNCC 4 cut(s) 577, 578, 601, 602
BmiI GGNNCC 2 cut(s) 579, 603
BmrFI CCNGG 1 cut(s) 388
BmsI GCATC 3 cut(s) 200, 303, 403
BplI GAGNNNNNCTC 2 cut(s) 474, 506
BpuEI CTTGAG 2 cut(s) 386, 479
BpuMI CCSGG 1 cut(s) 388
BsaAI YACGTR 2 cut(s) 39, 608
BsaBI GATNNNNATC 1 cut(s) 9
BsaJI CCNNGG 2 cut(s) 186, 238
BsaWI WCCGGW 2 cut(s) 220, 257
Bsc4I CCNNNNNNNGG 2 cut(s) 393, 599
Bse118I RCCGGY 1 cut(s) 220
Bse1I ACTGG 1 cut(s) 169
Bse3DI GCAATG 1 cut(s) 525
Bse8I GATNNNNATC 1 cut(s) 9
BseDI CCNNGG 2 cut(s) 186, 238
BseJI GATNNNNATC 1 cut(s) 9
BseLI CCNNNNNNNGG 2 cut(s) 393, 599
BseMI GCAATG 1 cut(s) 525
BseMII CTCAG 1 cut(s) 75
BseNI ACTGG 1 cut(s) 169
BseRI GAGGAG 2 cut(s) 482, 528
BseSI GKGCMC 2 cut(s) 581, 605
BseXI GCAGC 1 cut(s) 221
BshFI GGCC 5 cut(s) 185, 237, 332, 579, 603
BshTI ACCGGT 1 cut(s) 220
BsiSI CCGG 3 cut(s) 221, 258, 387
BslFI GGGAC 1 cut(s) 410
BslI CCNNNNNNNGG 2 cut(s) 393, 599
BsmAI GTCTC 2 cut(s) 336, 449
BsmFI GGGAC 1 cut(s) 410
BsnI GGCC 5 cut(s) 185, 237, 332, 579, 603
Bsp120I GGGCCC 2 cut(s) 577, 601
Bsp1286I GDGCHC 2 cut(s) 581, 605
Bsp143I GATC 4 cut(s) 51, 64, 370, 615
Bsp19I CCATGG 1 cut(s) 186
BspACI CCGC 2 cut(s) 243, 465
BspANI GGCC 5 cut(s) 185, 237, 332, 579, 603
BspCNI CTCAG 1 cut(s) 76
BspLI GGNNCC 2 cut(s) 579, 603
BspPI GGATC 2 cut(s) 46, 378
BsrBI CCGCTC 1 cut(s) 467
BsrDI GCAATG 1 cut(s) 525
BsrFI RCCGGY 1 cut(s) 220
BsrI ACTGG 1 cut(s) 169
BssAI RCCGGY 1 cut(s) 220
BssECI CCNNGG 2 cut(s) 186, 238
BssMI GATC 4 cut(s) 51, 64, 370, 615
BssT1I CCWWGG 1 cut(s) 186
Bst4CI ACNGT 1 cut(s) 407
Bst6I CTCTTC 2 cut(s) 116, 441
BstBAI YACGTR 2 cut(s) 39, 608
BstDEI CTNAG 3 cut(s) 48, 84, 536
BstDSI CCRYGG 1 cut(s) 186
BstEII GGTNACC 1 cut(s) 223
BstKTI GATC 4 cut(s) 54, 67, 373, 618
BstMAI GTCTC 2 cut(s) 336, 449
BstMBI GATC 4 cut(s) 51, 64, 370, 615
BstMWI GCNNNNNNNGC 4 cut(s) 197, 206, 329, 609
BstPI GGTNACC 1 cut(s) 223
BstSCI CCNGG 1 cut(s) 386
BstSLI GKGCMC 2 cut(s) 581, 605
BstV1I GCAGC 1 cut(s) 221
BstX2I RGATCY 3 cut(s) 51, 370, 615
BstYI RGATCY 3 cut(s) 51, 370, 615
BsuRI GGCC 5 cut(s) 185, 237, 332, 579, 603
BtgI CCRYGG 1 cut(s) 186
BtsI GCAGTG 1 cut(s) 543
BtsIMutI CAGTG 1 cut(s) 543
CaiI CAGNNNCTG 1 cut(s) 149
Cfr10I RCCGGY 1 cut(s) 220
Cfr13I GGNCC 4 cut(s) 577, 578, 601, 602
CseI GACGC 1 cut(s) 340
Csp6I GTAC 2 cut(s) 337, 403
CspAI ACCGGT 1 cut(s) 220
CspCI CAANNNNNGTGG 2 cut(s) 579, 614
CviAII CATG 2 cut(s) 56, 187
CviQI GTAC 2 cut(s) 337, 403
DdeI CTNAG 3 cut(s) 48, 84, 536
DpnI GATC 4 cut(s) 53, 66, 372, 617
DpnII GATC 4 cut(s) 51, 64, 370, 615
DrdI GACNNNNNNGTC 1 cut(s) 350
DseDI GACNNNNNNGTC 1 cut(s) 350
EaeI YGGCCR 1 cut(s) 330
Eam1104I CTCTTC 2 cut(s) 116, 441
EarI CTCTTC 2 cut(s) 116, 441
EciI GGCGGA 1 cut(s) 258
Eco130I CCWWGG 1 cut(s) 186
Eco24I GRGCYC 2 cut(s) 581, 605
Eco57I CTGAAG 1 cut(s) 422
Eco72I CACGTG 2 cut(s) 39, 608
Eco91I GGTNACC 1 cut(s) 223
EcoO109I RGGNCCY 1 cut(s) 577
EcoO65I GGTNACC 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 186
EcoT38I GRGCYC 2 cut(s) 581, 605
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 2 cut(s) 59, 190
FaiI YATR 3 cut(s) 10, 57, 188
FalI AAGNNNNNCTT 4 cut(s) 383, 415, 470, 502
FaqI GGGAC 1 cut(s) 410
FatI CATG 2 cut(s) 55, 186
FblI GTMKAC 2 cut(s) 165, 354
Fnu4HI GCNGC 2 cut(s) 210, 465
FriOI GRGCYC 2 cut(s) 581, 605
Fsp4HI GCNGC 2 cut(s) 210, 465
FspBI CTAG 1 cut(s) 206
GluI GCNGC 2 cut(s) 210, 465
HaeIII GGCC 5 cut(s) 185, 237, 332, 579, 603
HapII CCGG 3 cut(s) 221, 258, 387
HgaI GACGC 1 cut(s) 340
Hin1II CATG 2 cut(s) 59, 190
HinfI GANTC 1 cut(s) 5
HpaII CCGG 3 cut(s) 221, 258, 387
HphI GGTGA 4 cut(s) 217, 247, 414, 466
Hpy166II GTNNAC 2 cut(s) 166, 355
Hpy188I TCNGA 4 cut(s) 299, 412, 441, 553
Hpy188III TCNNGA 4 cut(s) 143, 313, 502, 571
Hpy8I GTNNAC 2 cut(s) 166, 355
Hpy99I CGWCG 2 cut(s) 557, 560
HpyAV CCTTC 1 cut(s) 568
HpyCH4III ACNGT 1 cut(s) 407
HpyCH4IV ACGT 3 cut(s) 38, 558, 607
HpyCH4V TGCA 2 cut(s) 59, 107
HpyF10VI GCNNNNNNNGC 4 cut(s) 197, 206, 329, 609
HpyF3I CTNAG 3 cut(s) 48, 84, 536
HpySE526I ACGT 3 cut(s) 38, 558, 607
Hsp92II CATG 2 cut(s) 59, 190
Kzo9I GATC 4 cut(s) 51, 64, 370, 615
LmnI GCTCC 3 cut(s) 254, 472, 515
LpnPI CCDG 9 cut(s) 156, 182, 182, 234, 271, 298, 302, 359, 400
Lsp1109I GCAGC 1 cut(s) 221
LweI GCATC 3 cut(s) 200, 303, 403
MaeI CTAG 1 cut(s) 206
MaeII ACGT 3 cut(s) 38, 558, 607
MaeIII GTNAC 1 cut(s) 223
MalI GATC 4 cut(s) 53, 66, 372, 617
MbiI CCGCTC 1 cut(s) 467
MboI GATC 4 cut(s) 51, 64, 370, 615
MboII GAAGA 2 cut(s) 133, 428
MflI RGATCY 3 cut(s) 51, 370, 615
MhlI GDGCHC 2 cut(s) 581, 605
MlsI TGGCCA 1 cut(s) 332
MluNI TGGCCA 1 cut(s) 332
MmeI TCCRAC 1 cut(s) 576
Mox20I TGGCCA 1 cut(s) 332
MscI TGGCCA 1 cut(s) 332
MseI TTAA 2 cut(s) 102, 231
Msp20I TGGCCA 1 cut(s) 332
MspI CCGG 3 cut(s) 221, 258, 387
MspR9I CCNGG 1 cut(s) 388
MwoI GCNNNNNNNGC 4 cut(s) 197, 206, 329, 609
NciI CCSGG 1 cut(s) 388
NcoI CCATGG 1 cut(s) 186
NdeII GATC 4 cut(s) 51, 64, 370, 615
NlaIII CATG 2 cut(s) 59, 190
NlaIV GGNNCC 2 cut(s) 579, 603
NmeAIII GCCGAG 1 cut(s) 219
NmuCI GTSAC 1 cut(s) 223
PfeI GAWTC 1 cut(s) 5
PinAI ACCGGT 1 cut(s) 220
PkrI GCNGC 2 cut(s) 211, 466
PmaCI CACGTG 2 cut(s) 39, 608
PmlI CACGTG 2 cut(s) 39, 608
Ppu21I YACGTR 2 cut(s) 39, 608
PspCI CACGTG 2 cut(s) 39, 608
PspEI GGTNACC 1 cut(s) 223
PspN4I GGNNCC 2 cut(s) 579, 603
PspOMI GGGCCC 2 cut(s) 577, 601
PspPI GGNCC 4 cut(s) 577, 578, 601, 602
PstNI CAGNNNCTG 1 cut(s) 149
PsuI RGATCY 3 cut(s) 51, 370, 615
RsaI GTAC 2 cut(s) 338, 404
RsaNI GTAC 2 cut(s) 337, 403
SaqAI TTAA 2 cut(s) 102, 231
SatI GCNGC 2 cut(s) 210, 465
Sau3AI GATC 4 cut(s) 51, 64, 370, 615
Sau96I GGNCC 4 cut(s) 577, 578, 601, 602
ScrFI CCNGG 1 cut(s) 388
SduI GDGCHC 2 cut(s) 581, 605
SfaNI GCATC 3 cut(s) 200, 303, 403
SmlI CTYRAG 2 cut(s) 365, 494
SmoI CTYRAG 2 cut(s) 365, 494
SsiI CCGC 2 cut(s) 243, 465
SspMI CTAG 1 cut(s) 206
StyD4I CCNGG 1 cut(s) 386
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 1 cut(s) 407
TaiI ACGT 3 cut(s) 41, 561, 610
TauI GCSGC 1 cut(s) 467
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 2 cut(s) 102, 231
Tru9I TTAA 2 cut(s) 102, 231
TscAI CASTG 1 cut(s) 550
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 1 cut(s) 209
Tsp45I GTSAC 1 cut(s) 223
TspGWI ACGGA 1 cut(s) 354
TspRI CASTG 1 cut(s) 550
XcmI CCANNNNNNNNNTGG 1 cut(s) 193
XmiI GTMKAC 2 cut(s) 165, 354
XspI CTAG 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.