pycom17g20770

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0012640
Physical Location & Seq
Reverse (-)
19201736 .. 19204856
3121 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1560 bp
ATGCGTTTAACTCAGGAACAAGAACGACTGAGGGCAGTAAACGCACGCTCTCTTCTGCGGAAGTTTCCCCAGAGTCTCCAACGACGGCAACCTCCGGTTTTAGTATTCTGGGGTGGCTTCTTAACTTCAAAAATGCCTAAAGTTAGAAGAGATAGTTCCTTGTCTCTTGATCGATCTAGGGCATCTCCTTATCCGTGCACCTCTGATGATTCTGATGCTTACAATCTGAAAAATCCTTTGGAAAGCGAAGATAACGCAAAAGAGTGGGAAGAAGCTAGATGCCCCATCTGCATGGAACATGCACACAATGCGGTTCTTTTAAAATGTTCATCTCATGAGAAGGGCTGCAGGCCTTTCATGTGCAATACAAGCTATCGGCACTCAAATTGCTTTGACCAGTTTTGTAAGTCATCTTCGCCTTGCTGCTCAACAGTTATGTTGCAGGAACTTCCTCTCACACACACCTCTCACATTGCTAGCGAGGAACAGTCGTCGCCTGGGCAAAGCAGCCCCTGTGGCAGTCAATTGGATTTGAAGCCCGTTTGCCCTCTTTGTCGTGGAGAGATCTATGGGTATGTTGTTGTAGAGGCTGCTCGCCACTTCATGAATGCCAAAGTGAGGAATTGCTCTTCTGAGACATGTGACTTTAGTGGGACTTATTCAGAACTGAGGAAGCATGCTAGGTCTGAACATCCTTCTGTCCGTCCATCTGAGGTGGATCCAACTCGACATAGTGATTGGGTAAGGTTGGAGCGTGAAAGGGACTTGGAAGATGTTCTTAGCTTGGTCCAACAAGGACCCGGAGATGAACTTCTTGATGATTCATCAGGGGATCTTAATTCCTGGATGTCGACCGTGTTCGCTGCAATGTTCCGTTCTCTTGAGGTCATGCTTGTGACTCGTTTGATGGATTCTTCAAGTGGCTCTTCAAGTGGCAGAGAGCAGCAGACGCATAACCGCAGATCAGGAAGGATATCCAGGGTTCGTTATGACAATGATACCATTCCTTCCACTAGACGGAGCAACAATGTATCAGATATTACATCTCGTCCCAGACGTCTGCGATGGCGGGCACTGAATGATGATGTGGGGACTAGCCGTTCCAGATGGGGTCGCAATTCCATGTCTGGGGAGGCTGGTGGTCATGCGCCTAGATGGGGCAACAGTTTGTTATCAGAGCAAAATAGCCGTACCCCAAGATGGGGCAACAACTCCTTGTCAGAGGAAGCTAACCATGCCGCTAGATGGGGCAACAATCCCTTATCAGAGGAGACCAGTCGCGCCTCTAGAATGGGCAACAACTCCTTAACGGAGGAGACCAGTCGTTGGCCTAGATGGAGCAACAACACCTTACCAGAAGAAACCAGTCGTGCTTCTATATGGGGCAACAACTCCTTACGTGAAGAAACCAATCGTGCTCCTAGATGGGGCAACAACTCCTTAACTGAAGAGACCAGTCGTGCTCCTAGATGGGGCAATGCTTCAGCACCTGAAAATACCCCTCGTACTGGAAGGCTGCGCTGGAGAAATCAAAGATGGCCAACAAATAACAGGCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

520

Amino Acids

59.09

Weight (kDa)

9.14

Isoelectric Point (pI)

75.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 90 - 261 3.2e-65 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1060
AccB7I CCANNNNNTGG 1 cut(s) 1326
AccI GTMKAC 1 cut(s) 851
AccII CGCG 1 cut(s) 1281
AciI CCGC 6 cut(s) 58, 311, 958, 1069, 1239, 1555
AclWI GGATC 3 cut(s) 713, 726, 840
AcoI YGGCCR 1 cut(s) 1538
AcuI CTGAAG 2 cut(s) 1467, 1467
AcyI GRCGYC 1 cut(s) 1057
AfaI GTAC 2 cut(s) 1192, 1507
AflIII ACRYGT 1 cut(s) 638
AgsI TTSAA 4 cut(s) 129, 535, 918, 930
AjnI CCWGG 3 cut(s) 496, 842, 977
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
AloI GAACNNNNNNTCC 2 cut(s) 1084, 1116
AluBI AGCT 4 cut(s) 275, 372, 783, 1229
AluI AGCT 4 cut(s) 275, 372, 783, 1229
Alw21I GWGCWC 3 cut(s) 200, 1420, 1465
Alw26I GTCTC 6 cut(s) 80, 168, 629, 1265, 1310, 1445
Alw44I GTGCAC 1 cut(s) 196
AlwI GGATC 3 cut(s) 713, 726, 840
AlwNI CAGNNNCTG 2 cut(s) 513, 1490
AoxI GGCC 3 cut(s) 350, 1328, 1538
ApaLI GTGCAC 1 cut(s) 196
ApeKI GCWGC 7 cut(s) 345, 423, 507, 590, 863, 943, 1516
Asp700I GAANNNNTTC 1 cut(s) 774
AspLEI GCGC 3 cut(s) 1150, 1283, 1521
AspS9I GGNCC 2 cut(s) 787, 797
AsuC2I CCSGG 1 cut(s) 801
AsuNHI GCTAGC 1 cut(s) 476
AvaII GGWCC 2 cut(s) 787, 797
BaeGI GKGCMC 2 cut(s) 200, 1075
BalI TGGCCA 1 cut(s) 1540
BamHI GGATCC 1 cut(s) 718
BarI GAAGNNNNNNTAC 2 cut(s) 397, 429
Bbv12I GWGCWC 3 cut(s) 200, 1420, 1465
BbvI GCAGC 7 cut(s) 332, 410, 519, 577, 850, 955, 1503
BceAI ACGGC 3 cut(s) 101, 1083, 1173
BciT130I CCWGG 3 cut(s) 498, 844, 979
BcnI CCSGG 1 cut(s) 801
BcoDI GTCTC 6 cut(s) 80, 168, 629, 1265, 1310, 1445
BfmI CTRYAG 1 cut(s) 346
BglI GCCNNNNNGGC 1 cut(s) 516
BglII AGATCT 1 cut(s) 564
BisI GCNGC 8 cut(s) 346, 424, 508, 591, 864, 944, 1239, 1517
BlsI GCNGC 8 cut(s) 347, 425, 509, 592, 865, 945, 1240, 1518
Bme1390I CCNGG 4 cut(s) 498, 801, 844, 979
Bme18I GGWCC 2 cut(s) 787, 797
BmgT120I GGNCC 2 cut(s) 787, 797
BmiI GGNNCC 2 cut(s) 720, 799
BmrFI CCNGG 4 cut(s) 498, 801, 844, 979
BmsI GCATC 3 cut(s) 191, 205, 269
BmtI GCTAGC 1 cut(s) 480
BpmI CTGGAG 1 cut(s) 1543
BpuEI CTTGAG 1 cut(s) 902
BpuMI CCSGG 1 cut(s) 801
Bsa29I ATCGAT 1 cut(s) 172
BsaAI YACGTR 1 cut(s) 1400
BsaHI GRCGYC 1 cut(s) 1057
BsaI GGTCTC 3 cut(s) 1265, 1310, 1445
BsaJI CCNNGG 2 cut(s) 497, 978
BsaWI WCCGGW 1 cut(s) 94
Bse1I ACTGG 6 cut(s) 397, 1275, 1320, 1365, 1455, 1513
Bse3DI GCAATG 3 cut(s) 471, 873, 1483
BseBI CCWGG 3 cut(s) 498, 844, 979
BseCI ATCGAT 1 cut(s) 172
BseDI CCNNGG 2 cut(s) 497, 978
BseGI GGATG 2 cut(s) 691, 852
BseMI GCAATG 3 cut(s) 471, 873, 1483
BseMII CTCAG 5 cut(s) 20, 26, 624, 659, 702
BseNI ACTGG 6 cut(s) 397, 1275, 1320, 1365, 1455, 1513
BseRI GAGGAG 2 cut(s) 1283, 1328
BseSI GKGCMC 2 cut(s) 200, 1075
BseXI GCAGC 7 cut(s) 332, 410, 519, 577, 850, 955, 1503
Bsh1236I CGCG 1 cut(s) 1281
Bsh1285I CGRYCG 1 cut(s) 855
BshFI GGCC 3 cut(s) 352, 1330, 1540
BshVI ATCGAT 1 cut(s) 172
BsiEI CGRYCG 1 cut(s) 855
BsiHKAI GWGCWC 3 cut(s) 200, 1420, 1465
BsiSI CCGG 2 cut(s) 95, 801
BslFI GGGAC 4 cut(s) 667, 776, 1035, 1105
BsmAI GTCTC 6 cut(s) 80, 168, 629, 1265, 1310, 1445
BsmFI GGGAC 4 cut(s) 667, 776, 1035, 1105
BsmI GAATGC 1 cut(s) 613
BsnI GGCC 3 cut(s) 352, 1330, 1540
Bso31I GGTCTC 3 cut(s) 1265, 1310, 1445
Bsp1286I GDGCHC 4 cut(s) 200, 1075, 1420, 1465
Bsp143I GATC 6 cut(s) 169, 173, 564, 718, 832, 962
BspACI CCGC 6 cut(s) 58, 311, 958, 1069, 1239, 1555
BspANI GGCC 3 cut(s) 352, 1330, 1540
BspCNI CTCAG 5 cut(s) 21, 25, 625, 660, 703
BspDI ATCGAT 1 cut(s) 172
BspFNI CGCG 1 cut(s) 1281
BspHI TCATGA 2 cut(s) 334, 603
BspLI GGNNCC 2 cut(s) 720, 799
BspMAI CTGCAG 1 cut(s) 350
BspOI GCTAGC 1 cut(s) 480
BspPI GGATC 3 cut(s) 713, 726, 840
BspQI GCTCTTC 2 cut(s) 634, 931
BspTNI GGTCTC 3 cut(s) 1265, 1310, 1445
BsrDI GCAATG 3 cut(s) 471, 873, 1483
BsrI ACTGG 6 cut(s) 397, 1275, 1320, 1365, 1455, 1513
BssECI CCNNGG 2 cut(s) 497, 978
BssMI GATC 6 cut(s) 169, 173, 564, 718, 832, 962
BssNI GRCGYC 1 cut(s) 1057
Bst2UI CCWGG 3 cut(s) 498, 844, 979
Bst4CI ACNGT 4 cut(s) 433, 489, 856, 1166
Bst6I CTCTTC 5 cut(s) 57, 142, 634, 931, 1443
BstACI GRCGYC 1 cut(s) 1057
BstAPI GCANNNNNTGC 1 cut(s) 308
BstBAI YACGTR 1 cut(s) 1400
BstC8I GCNNGC 6 cut(s) 46, 350, 478, 595, 678, 1071
BstDEI CTNAG 6 cut(s) 12, 29, 633, 668, 711, 779
BstF5I GGATG 2 cut(s) 691, 852
BstFNI CGCG 1 cut(s) 1281
BstHHI GCGC 3 cut(s) 1150, 1283, 1521
BstKTI GATC 6 cut(s) 172, 176, 567, 721, 835, 965
BstMAI GTCTC 6 cut(s) 80, 168, 629, 1265, 1310, 1445
BstMBI GATC 6 cut(s) 169, 173, 564, 718, 832, 962
BstMCI CGRYCG 1 cut(s) 855
BstMWI GCNNNNNNNGC 7 cut(s) 41, 288, 308, 369, 516, 949, 1235
BstNI CCWGG 3 cut(s) 498, 844, 979
BstNSI RCATGY 3 cut(s) 302, 642, 680
BstSCI CCNGG 4 cut(s) 496, 799, 842, 977
BstSFI CTRYAG 1 cut(s) 346
BstSLI GKGCMC 2 cut(s) 200, 1075
BstUI CGCG 1 cut(s) 1281
BstV1I GCAGC 7 cut(s) 332, 410, 519, 577, 850, 955, 1503
BstX2I RGATCY 3 cut(s) 564, 718, 832
BstXI CCANNNNNNTGG 1 cut(s) 292
BstYI RGATCY 3 cut(s) 564, 718, 832
Bsu15I ATCGAT 1 cut(s) 172
BsuRI GGCC 3 cut(s) 352, 1330, 1540
BsuTUI ATCGAT 1 cut(s) 172
BtgZI GCGATG 1 cut(s) 1078
BtsCI GGATG 2 cut(s) 691, 852
BtsIMutI CAGTG 1 cut(s) 1073
Cac8I GCNNGC 6 cut(s) 46, 350, 478, 595, 678, 1071
CaiI CAGNNNCTG 2 cut(s) 513, 1490
CciI TCATGA 2 cut(s) 334, 603
CfoI GCGC 3 cut(s) 1150, 1283, 1521
Cfr13I GGNCC 2 cut(s) 787, 797
ClaI ATCGAT 1 cut(s) 172
CseI GACGC 1 cut(s) 958
Csp6I GTAC 2 cut(s) 1191, 1506
CviQI GTAC 2 cut(s) 1191, 1506
DdeI CTNAG 6 cut(s) 12, 29, 633, 668, 711, 779
DpnI GATC 6 cut(s) 171, 175, 566, 720, 834, 964
DpnII GATC 6 cut(s) 169, 173, 564, 718, 832, 962
DraI TTTAAA 1 cut(s) 321
EaeI YGGCCR 1 cut(s) 1538
Eam1104I CTCTTC 5 cut(s) 57, 142, 634, 931, 1443
EarI CTCTTC 5 cut(s) 57, 142, 634, 931, 1443
Eco147I AGGCCT 1 cut(s) 352
Eco31I GGTCTC 3 cut(s) 1265, 1310, 1445
Eco32I GATATC 1 cut(s) 975
Eco47I GGWCC 2 cut(s) 787, 797
Eco57I CTGAAG 2 cut(s) 1467, 1467
EcoO109I RGGNCCY 1 cut(s) 797
EcoRII CCWGG 3 cut(s) 496, 842, 977
EcoRV GATATC 1 cut(s) 975
FalI AAGNNNNNCTT 4 cut(s) 762, 794, 910, 942
FaqI GGGAC 4 cut(s) 667, 776, 1035, 1105
FauI CCCGC 1 cut(s) 1062
FblI GTMKAC 1 cut(s) 851
Fnu4HI GCNGC 8 cut(s) 346, 424, 508, 591, 864, 944, 1239, 1517
FokI GGATG 2 cut(s) 678, 859
Fsp4HI GCNGC 8 cut(s) 346, 424, 508, 591, 864, 944, 1239, 1517
GlaI GCGC 3 cut(s) 1149, 1282, 1520
GluI GCNGC 8 cut(s) 346, 424, 508, 591, 864, 944, 1239, 1517
GsuI CTGGAG 1 cut(s) 1543
HaeIII GGCC 3 cut(s) 352, 1330, 1540
HapII CCGG 2 cut(s) 95, 801
HgaI GACGC 1 cut(s) 958
HhaI GCGC 3 cut(s) 1150, 1283, 1521
Hin1I GRCGYC 1 cut(s) 1057
Hin6I GCGC 3 cut(s) 1148, 1281, 1519
HinP1I GCGC 3 cut(s) 1148, 1281, 1519
HincII GTYRAC 1 cut(s) 852
HindII GTYRAC 1 cut(s) 852
HinfI GANTC 5 cut(s) 73, 209, 821, 898, 911
HpaII CCGG 2 cut(s) 95, 801
Hpy166II GTNNAC 3 cut(s) 40, 198, 852
Hpy188III TCNNGA 9 cut(s) 14, 167, 335, 604, 815, 881, 966, 1104, 1287
Hpy8I GTNNAC 3 cut(s) 40, 198, 852
Hpy99I CGWCG 2 cut(s) 87, 496
HpyAV CCTTC 5 cut(s) 334, 705, 963, 1017, 1506
HpyCH4III ACNGT 4 cut(s) 433, 489, 856, 1166
HpyCH4IV ACGT 2 cut(s) 1057, 1399
HpyCH4V TGCA 7 cut(s) 198, 291, 302, 348, 363, 442, 866
HpyF10VI GCNNNNNNNGC 7 cut(s) 41, 288, 308, 369, 516, 949, 1235
HpyF3I CTNAG 6 cut(s) 12, 29, 633, 668, 711, 779
HpySE526I ACGT 2 cut(s) 1057, 1399
Hsp92I GRCGYC 1 cut(s) 1057
HspAI GCGC 3 cut(s) 1148, 1281, 1519
Kzo9I GATC 6 cut(s) 169, 173, 564, 718, 832, 962
LguI GCTCTTC 2 cut(s) 634, 931
LmnI GCTCC 5 cut(s) 751, 1020, 1338, 1423, 1468
Lsp1109I GCAGC 7 cut(s) 332, 410, 519, 577, 850, 955, 1503
LweI GCATC 3 cut(s) 191, 205, 269
MaeII ACGT 2 cut(s) 1057, 1399
MaeIII GTNAC 2 cut(s) 641, 895
MalI GATC 6 cut(s) 171, 175, 566, 720, 834, 964
MboI GATC 6 cut(s) 169, 173, 564, 718, 832, 962
MfeI CAATTG 1 cut(s) 524
MflI RGATCY 3 cut(s) 564, 718, 832
MhlI GDGCHC 4 cut(s) 200, 1075, 1420, 1465
MlsI TGGCCA 1 cut(s) 1540
MluCI AATT 5 cut(s) 385, 524, 622, 838, 1117
MluNI TGGCCA 1 cut(s) 1540
MlyI GAGTC 2 cut(s) 82, 892
MmeI TCCRAC 4 cut(s) 103, 729, 746, 814
Mox20I TGGCCA 1 cut(s) 1540
MroXI GAANNNNTTC 1 cut(s) 774
MscI TGGCCA 1 cut(s) 1540
MseI TTAA 6 cut(s) 8, 122, 320, 837, 1307, 1442
MslI CAYNNNNRTG 2 cut(s) 290, 893
Msp20I TGGCCA 1 cut(s) 1540
MspI CCGG 2 cut(s) 95, 801
MspR9I CCNGG 4 cut(s) 498, 801, 844, 979
MunI CAATTG 1 cut(s) 524
Mva1269I GAATGC 1 cut(s) 613
MvaI CCWGG 3 cut(s) 498, 844, 979
MvnI CGCG 1 cut(s) 1281
MwoI GCNNNNNNNGC 7 cut(s) 41, 288, 308, 369, 516, 949, 1235
NciI CCSGG 1 cut(s) 801
NdeII GATC 6 cut(s) 169, 173, 564, 718, 832, 962
NheI GCTAGC 1 cut(s) 476
NlaIV GGNNCC 2 cut(s) 720, 799
NmuCI GTSAC 2 cut(s) 641, 895
NspI RCATGY 3 cut(s) 302, 642, 680
PaeI GCATGC 1 cut(s) 680
PagI TCATGA 2 cut(s) 334, 603
PceI AGGCCT 1 cut(s) 352
PciI ACATGT 1 cut(s) 638
PciSI GCTCTTC 2 cut(s) 634, 931
PcsI WCGNNNNNNNCGW 1 cut(s) 1054
PctI GAATGC 1 cut(s) 613
PdmI GAANNNNTTC 1 cut(s) 774
PfeI GAWTC 3 cut(s) 209, 821, 911
PflMI CCANNNNNTGG 1 cut(s) 1326
PfoI TCCNGGA 1 cut(s) 842
PkrI GCNGC 8 cut(s) 347, 425, 509, 592, 865, 945, 1240, 1518
PleI GAGTC 2 cut(s) 81, 892
PpsI GAGTC 2 cut(s) 81, 892
Ppu21I YACGTR 1 cut(s) 1400
PpuMI RGGWCCY 1 cut(s) 797
PscI ACATGT 1 cut(s) 638
Psp5II RGGWCCY 1 cut(s) 797
Psp6I CCWGG 3 cut(s) 496, 842, 977
PspGI CCWGG 3 cut(s) 496, 842, 977
PspN4I GGNNCC 2 cut(s) 720, 799
PspPI GGNCC 2 cut(s) 787, 797
PspPPI RGGWCCY 1 cut(s) 797
PstI CTGCAG 1 cut(s) 350
PstNI CAGNNNCTG 2 cut(s) 513, 1490
PsuI RGATCY 3 cut(s) 564, 718, 832
RsaI GTAC 2 cut(s) 1192, 1507
RsaNI GTAC 2 cut(s) 1191, 1506
RseI CAYNNNNRTG 2 cut(s) 290, 893
SalI GTCGAC 1 cut(s) 850
SapI GCTCTTC 2 cut(s) 634, 931
SaqAI TTAA 6 cut(s) 8, 122, 320, 837, 1307, 1442
SatI GCNGC 8 cut(s) 346, 424, 508, 591, 864, 944, 1239, 1517
Sau3AI GATC 6 cut(s) 169, 173, 564, 718, 832, 962
Sau96I GGNCC 2 cut(s) 787, 797
SchI GAGTC 2 cut(s) 82, 892
ScrFI CCNGG 4 cut(s) 498, 801, 844, 979
SduI GDGCHC 4 cut(s) 200, 1075, 1420, 1465
SfaNI GCATC 3 cut(s) 191, 205, 269
SfcI CTRYAG 1 cut(s) 346
SinI GGWCC 2 cut(s) 787, 797
SmiMI CAYNNNNRTG 2 cut(s) 290, 893
SmlI CTYRAG 1 cut(s) 881
SmoI CTYRAG 1 cut(s) 881
SphI GCATGC 1 cut(s) 680
Sse9I AATT 5 cut(s) 385, 524, 622, 838, 1117
SseBI AGGCCT 1 cut(s) 352
SsiI CCGC 6 cut(s) 58, 311, 958, 1069, 1239, 1555
StuI AGGCCT 1 cut(s) 352
StyD4I CCNGG 4 cut(s) 496, 799, 842, 977
TaaI ACNGT 4 cut(s) 433, 489, 856, 1166
TaiI ACGT 2 cut(s) 1060, 1402
TaqI TCGA 3 cut(s) 172, 727, 851
TasI AATT 5 cut(s) 385, 524, 622, 838, 1117
TauI GCSGC 1 cut(s) 1241
TfiI GAWTC 3 cut(s) 209, 821, 911
Tru1I TTAA 6 cut(s) 8, 122, 320, 837, 1307, 1442
Tru9I TTAA 6 cut(s) 8, 122, 320, 837, 1307, 1442
TscAI CASTG 1 cut(s) 1080
TseFI GTSAC 2 cut(s) 641, 895
TseI GCWGC 7 cut(s) 345, 423, 507, 590, 863, 943, 1516
Tsp45I GTSAC 2 cut(s) 641, 895
TspDTI ATGAA 6 cut(s) 318, 346, 592, 620, 813, 822
TspGWI ACGGA 5 cut(s) 183, 692, 863, 1033, 1325
TspRI CASTG 1 cut(s) 1080
Van91I CCANNNNNTGG 1 cut(s) 1326
VneI GTGCAC 1 cut(s) 196
VpaK11BI GGWCC 2 cut(s) 787, 797
XbaI TCTAGA 1 cut(s) 1286
XceI RCATGY 3 cut(s) 302, 642, 680
XmiI GTMKAC 1 cut(s) 851
XmnI GAANNNNTTC 1 cut(s) 774
ZraI GACGTC 1 cut(s) 1058
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.