Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr17

Orthogroup ID

OG0006290

Physical Location & Seq

Genomic Coordinates Forward (+)

19476410 .. 19478347

Gene Structure 1938 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom17g21050.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1710 bp

ATGCATAACAAAGAGAATACAAGCCGGGGGTGCAATCCGATCCGGTCTCTCGATCTTACGGCAACACGAAGACAAGCAAAATCCACAGTGACACTGCTGCTTCTTCTTCTTCTTCTTTACCCACTCACCAAAATGTCCCTGAGATCTCTCCCCTCTAAACCCAAAACCCTTCACTCTATCTTCCACTCTCTTCTCTCACCCTCCAAACCCAACGCTCACCGCCACCCCTTCTCTTCTCTCACCCACCACAACCCGCCATCTCCACCATCACCGCACCTGGTAAGCGAGTTCTCCCGCATCCTCAGCGACCACAGGAACCCTCACCACGACTTGGAGCTCTCCCTCAACGCTTTGTCTACCCAAATCTCCACAGACTTGGTTGAGCAAGTTCTGAAACGCTGCAAAAATCTCGGGCTTTCTGCACACAGATTCTTTCTTTGGGCTAAAACAATTCCGGGTTTTCGACATAGCGCTGAAAGCTATCACATTTTGATTGACATTTTAGGAAGTAGCAAGCAATATGCTGTGTTGTGGGATTTCCTCATAGAAGTGAGAGAGTCCAAGTGTTGTGAGATTGGCCCGGAAATTTTCTGGCTTATTTTTCGGGTTTATAGTAGAGCTAATTTGCCTCGCGACGCCATTCGAGCTTTAAATCGAATGGTTGAGTTTGGAATTAAGCCCAGTGTTCATGATCTTGATCAGCTTCTTTACACATTATGTAAAAGAAAGCATGTCAAGTATGCCCATGAATTTTTTGATAAAGTTAAGAGTGGGTTTGAGTTGAATGCGAAAACTTATAGTATTTTGATGAGAGGTTGGGGAGACGTTGGTGATTCGGACGAAGCACGTAAGCTGTTTGATGAAATGACTGAACGAGGATGTTTGGTTGATGTGCCTGCGTATAATAGTTATTTGGAGGCTCTGTGTAAAGGTGGGAAAGTTGACGAGGCGTATAAGATATTTCGGGAGATGGGGTCGAATGGAGTTGAACCGGATGCTTGTACCTATTCGATTTTCATTCGTGCTTATTGTGAGGTGGATGATATTCATTCAGTTTTTAGGGTGCTTGATAGGATGAGGAGATATAATCTTTTGCCCAATGTGTATACTTACAATTGTGTTATCAAGAAATTGTGTAAGAACGAAAAGGTGGAAGAGGCTTACCAACTTTTGGATGAAATGATTGAGAGGGGTGTTATGCCGGATACGTGGAGTTACAATGCAATCCAGGCTTATCATTGTGATCACTGCGAGGTTAATGGGTCTCTCAGGCTGTTATCTAGAATGGAAAAAGACAACTGCATGCCCGATCGACATACTTACAATATGGTGCTTAAGTTGCTAATCAGGATAGGAAGATTTGATAGGGCAACTGAAGTTTGGGAGAGTATGGGGAAGATAGGATTTTATCCTTCTGTTTCAACATACTCTGTCATGATTCATGGCTTATGCAAGAAGAAACGTAAACTAGAGGAGGCTTGTAAATACTTTGAGATAATGATCGATGAAGGTATACCGCCATACTCTTCTACTGTTGAGATGCTGAGTAACCGGATTAGGGGCTTAGGGCTCCTCGACCATATTGAGATACTTGCAAGTAAGATGGAACAAAGCACTTCTTGCTCAATACAAGAGTCGGCAAACTTGATGAGAGGTAATAAAGCTTATGTAGCATCAAGAAGTGTAGAGAGTGACTTTGAAAGTGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04212 map04212

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

570

Amino Acids

65.43

Weight (kDa)

8.42

Isoelectric Point (pI)

46.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	211 - 361	6.7e-10	Pentacotripeptide-repeat region of PRORP
PPR	PF01535	265 - 294	2.7e-06	PPR repeat
PPR_3	PF13812	284 - 343	1.5e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	292 - 324	1e-07	PPR repeat
PPR_2	PF13041	297 - 344	3.3e-16	PPR repeat family
PPR	PF01535	300 - 329	1.6e-06	PPR repeat
PPR_long	PF17177	314 - 408	1.3e-09	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	319 - 378	5.8e-11	Pentatricopeptide repeat domain
TPR_24	PF23276	327 - 397	1.8e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	327 - 360	3.2e-06	PPR repeat
PPR_2	PF13041	340 - 380	5.9e-10	PPR repeat family
PPR_long	PF17177	348 - 479	3.3e-09	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	354 - 409	4.7e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	363 - 394	4.8e-12	PPR repeat
PPR_2	PF13041	366 - 408	1.8e-14	PPR repeat family
PPR	PF01535	369 - 399	2.1e-08	PPR repeat
PPR_2	PF13041	401 - 448	1.4e-08	PPR repeat family
PPR_2	PF13041	440 - 485	1.8e-11	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0006290)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G52640
fragaria_vesca	FvH4_6g30140 FvH4_6g30140 FvH4_6g30140 FvH4_6g30140 FvH4_6g30140
malus_domestica	MD17G1208000.v1.1
prunus_persica	Prupe.3G082300_v2.0.a1 Prupe.3G082300_v2.0.a1
pyrus_communis	pycom17g21050
rosa_chinensis	RchiOBHm_Chr2g0136191 RchiOBHm_Chr2g0136301 RchiOBHm_Chr2g0136351
rosa_laevigata	RLG00000019513 RLG00000019521
rosa_multiflora	Rmu_sc0001998.1_g000030 Rmu_sc0002070.1_g000029
rosa_roxburghii	Rroxscaffold_2G00108380
rosa_rugosa	Rorug02G0328900 Rorug02G0329100 Rorug02G0329200
rosa_samantha	Rh2AG380400 Rh2AG380700 Rh2BG387500 Rh2CG366500 Rh2DG403700
rosa_wichuraiana	Rw2G031130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	331, 1171
AccI	GTMKAC	3 cut(s)	356, 1106, 1513
AccII	CGCG	1 cut(s)	633
AciI	CCGC	5 cut(s)	220, 254, 272, 295, 1517
AclWI	GGATC	1 cut(s)	34
AcsI	RAATTY	2 cut(s)	585, 749
AcuI	CTGAAG	1 cut(s)	1395
AcyI	GRCGYC	1 cut(s)	636
AfaI	GTAC	1 cut(s)	1003
AfeI	AGCGCT	1 cut(s)	472
AfiI	CCNNNNNNNGG	3 cut(s)	331, 1171, 1558
AflII	CTTAAG	1 cut(s)	1334
AgsI	TTSAA	4 cut(s)	784, 989, 1422, 1700
AjnI	CCWGG	2 cut(s)	276, 1227
AluBI	AGCT	7 cut(s)	337, 480, 620, 647, 703, 853, 1664
AluI	AGCT	7 cut(s)	337, 480, 620, 647, 703, 853, 1664
Alw21I	GWGCWC	1 cut(s)	339
Alw26I	GTCTC	3 cut(s)	51, 816, 1269
AlwI	GGATC	1 cut(s)	34
Ama87I	CYCGRG	1 cut(s)	410
Aor51HI	AGCGCT	1 cut(s)	472
AoxI	GGCC	1 cut(s)	577
ApeKI	GCWGC	2 cut(s)	97, 399
ApoI	RAATTY	2 cut(s)	585, 749
AspLEI	GCGC	1 cut(s)	473
AspS9I	GGNCC	1 cut(s)	578
AsuC2I	CCSGG	3 cut(s)	26, 456, 581
AsuHPI	GGTGA	7 cut(s)	118, 189, 209, 232, 261, 314, 842
AvaI	CYCGRG	1 cut(s)	410
BanII	GRGCYC	2 cut(s)	339, 1572
BbsI	GAAGAC	1 cut(s)	76
Bbv12I	GWGCWC	1 cut(s)	339
BbvCI	CCTCAGC	1 cut(s)	302
BbvI	GCAGC	2 cut(s)	84, 386
BccI	CCATC	4 cut(s)	265, 274, 964, 1597
BceAI	ACGGC	1 cut(s)	75
BcgI	CGANNNNNNTGC	2 cut(s)	391, 425
BciT130I	CCWGG	2 cut(s)	278, 1229
BciVI	GTATCC	1 cut(s)	1198
BclI	TGATCA	2 cut(s)	697, 1243
BcnI	CCSGG	3 cut(s)	26, 456, 581
BcoDI	GTCTC	3 cut(s)	51, 816, 1269
BfaI	CTAG	2 cut(s)	1281, 1469
BfoI	RGCGCY	1 cut(s)	474
BfrI	CTTAAG	1 cut(s)	1334
BfuI	GTATCC	1 cut(s)	1198
BglII	AGATCT	1 cut(s)	143
BisI	GCNGC	2 cut(s)	98, 400
BlsI	GCNGC	2 cut(s)	99, 401
Bme1390I	CCNGG	5 cut(s)	26, 278, 456, 581, 1229
BmeT110I	CYCGRG	1 cut(s)	410
BmgT120I	GGNCC	1 cut(s)	578
BmiI	GGNNCC	2 cut(s)	317, 1571
BmrFI	CCNGG	5 cut(s)	26, 278, 456, 581, 1229
BmrI	ACTGGG	1 cut(s)	675
BmsI	GCATC	4 cut(s)	306, 985, 1530, 1682
BmuI	ACTGGG	1 cut(s)	675
BpiI	GAAGAC	1 cut(s)	76
Bpu10I	CCTNAGC	2 cut(s)	302, 1564
BpuMI	CCSGG	3 cut(s)	26, 456, 581
Bsa29I	ATCGAT	1 cut(s)	1503
BsaAI	YACGTR	2 cut(s)	848, 1209
BsaBI	GATNNNNATC	2 cut(s)	696, 1499
BsaHI	GRCGYC	1 cut(s)	636
BsaI	GGTCTC	2 cut(s)	51, 1269
BsaJI	CCNNGG	1 cut(s)	25
BsaWI	WCCGGW	3 cut(s)	42, 991, 1551
BsaXI	ACNNNNNCTCC	4 cut(s)	908, 938, 959, 989
Bsc4I	CCNNNNNNNGG	3 cut(s)	331, 1171, 1558
Bse1I	ACTGG	1 cut(s)	681
Bse8I	GATNNNNATC	2 cut(s)	696, 1499
BseBI	CCWGG	2 cut(s)	278, 1229
BseCI	ATCGAT	1 cut(s)	1503
BseDI	CCNNGG	1 cut(s)	25
BseGI	GGATG	6 cut(s)	297, 884, 1000, 1045, 1080, 1180
BseJI	GATNNNNATC	2 cut(s)	696, 1499
BseLI	CCNNNNNNNGG	3 cut(s)	331, 1171, 1558
BseMII	CTCAG	4 cut(s)	131, 316, 1282, 1535
BseNI	ACTGG	1 cut(s)	681
BseRI	GAGGAG	3 cut(s)	1093, 1487, 1562
BseXI	GCAGC	2 cut(s)	84, 386
BsgI	GTGCAG	1 cut(s)	405
Bsh1236I	CGCG	1 cut(s)	633
Bsh1285I	CGRYCG	1 cut(s)	1312
BshFI	GGCC	1 cut(s)	579
BshVI	ATCGAT	1 cut(s)	1503
BsiEI	CGRYCG	1 cut(s)	1312
BsiHKAI	GWGCWC	1 cut(s)	339
BsiHKCI	CYCGRG	1 cut(s)	410
BsiSI	CCGG	7 cut(s)	25, 43, 455, 581, 992, 1202, 1552
BslFI	GGGAC	1 cut(s)	121
BslI	CCNNNNNNNGG	3 cut(s)	331, 1171, 1558
BsmAI	GTCTC	3 cut(s)	51, 816, 1269
BsmBI	CGTCTC	1 cut(s)	816
BsmFI	GGGAC	1 cut(s)	121
BsmI	GAATGC	1 cut(s)	790
BsnI	GGCC	1 cut(s)	579
Bso31I	GGTCTC	2 cut(s)	51, 1269
BsoBI	CYCGRG	1 cut(s)	410
Bsp1286I	GDGCHC	2 cut(s)	339, 1572
Bsp143I	GATC	8 cut(s)	39, 52, 143, 691, 697, 1243, 1309, 1500
Bsp68I	TCGCGA	1 cut(s)	633
BspACI	CCGC	5 cut(s)	220, 254, 272, 295, 1517
BspANI	GGCC	1 cut(s)	579
BspCNI	CTCAG	4 cut(s)	132, 315, 1281, 1536
BspDI	ATCGAT	1 cut(s)	1503
BspFNI	CGCG	1 cut(s)	633
BspHI	TCATGA	2 cut(s)	688, 1434
BspLI	GGNNCC	2 cut(s)	317, 1571
BspPI	GGATC	1 cut(s)	34
BspTI	CTTAAG	1 cut(s)	1334
BspTNI	GGTCTC	2 cut(s)	51, 1269
BsrI	ACTGG	1 cut(s)	681
BssECI	CCNNGG	1 cut(s)	25
BssMI	GATC	8 cut(s)	39, 52, 143, 691, 697, 1243, 1309, 1500
BssNAI	GTATAC	2 cut(s)	1107, 1514
BssNI	GRCGYC	1 cut(s)	636
Bst1107I	GTATAC	2 cut(s)	1107, 1514
Bst2UI	CCWGG	2 cut(s)	278, 1229
Bst4CI	ACNGT	2 cut(s)	88, 1534
Bst6I	CTCTTC	4 cut(s)	195, 238, 1149, 1531
BstACI	GRCGYC	1 cut(s)	636
BstAFI	CTTAAG	1 cut(s)	1334
BstAPI	GCANNNNNTGC	1 cut(s)	1620
BstBAI	YACGTR	2 cut(s)	848, 1209
BstC8I	GCNNGC	3 cut(s)	515, 897, 1304
BstDEI	CTNAG	5 cut(s)	140, 302, 1268, 1544, 1564
BstF5I	GGATG	6 cut(s)	297, 884, 1000, 1045, 1080, 1180
BstFNI	CGCG	1 cut(s)	633
BstH2I	RGCGCY	1 cut(s)	474
BstHHI	GCGC	1 cut(s)	473
BstKTI	GATC	8 cut(s)	42, 55, 146, 694, 700, 1246, 1312, 1503
BstMAI	GTCTC	3 cut(s)	51, 816, 1269
BstMBI	GATC	8 cut(s)	39, 52, 143, 691, 697, 1243, 1309, 1500
BstMCI	CGRYCG	1 cut(s)	1312
BstMWI	GCNNNNNNNGC	8 cut(s)	30, 303, 477, 644, 1229, 1339, 1620, 1670
BstNI	CCWGG	2 cut(s)	278, 1229
BstNSI	RCATGY	2 cut(s)	734, 1306
BstSCI	CCNGG	5 cut(s)	24, 276, 454, 579, 1227
BstUI	CGCG	1 cut(s)	633
BstV1I	GCAGC	2 cut(s)	84, 386
BstV2I	GAAGAC	1 cut(s)	76
BstX2I	RGATCY	1 cut(s)	143
BstXI	CCANNNNNNTGG	1 cut(s)	376
BstYI	RGATCY	1 cut(s)	143
BstZ17I	GTATAC	2 cut(s)	1107, 1514
Bsu15I	ATCGAT	1 cut(s)	1503
BsuI	GTATCC	1 cut(s)	1198
BsuRI	GGCC	1 cut(s)	579
BsuTUI	ATCGAT	1 cut(s)	1503
BtsCI	GGATG	6 cut(s)	297, 884, 1000, 1045, 1080, 1180
BtsI	GCAGTG	2 cut(s)	92, 1246
BtsIMutI	CAGTG	4 cut(s)	92, 93, 688, 1246
BtuMI	TCGCGA	1 cut(s)	633
Cac8I	GCNNGC	3 cut(s)	515, 897, 1304
CciI	TCATGA	2 cut(s)	688, 1434
CfoI	GCGC	1 cut(s)	473
Cfr13I	GGNCC	1 cut(s)	578
ClaI	ATCGAT	1 cut(s)	1503
CseI	GACGC	1 cut(s)	644
CsiI	ACCWGGT	1 cut(s)	276
Csp6I	GTAC	1 cut(s)	1002
CviAII	CATG	6 cut(s)	689, 731, 746, 1303, 1435, 1442
CviQI	GTAC	1 cut(s)	1002
DdeI	CTNAG	5 cut(s)	140, 302, 1268, 1544, 1564
DpnI	GATC	8 cut(s)	41, 54, 145, 693, 699, 1245, 1311, 1502
DpnII	GATC	8 cut(s)	39, 52, 143, 691, 697, 1243, 1309, 1500
DraI	TTTAAA	1 cut(s)	651
Eam1104I	CTCTTC	4 cut(s)	195, 238, 1149, 1531
EarI	CTCTTC	4 cut(s)	195, 238, 1149, 1531
Ecl136II	GAGCTC	1 cut(s)	337
Eco24I	GRGCYC	2 cut(s)	339, 1572
Eco31I	GGTCTC	2 cut(s)	51, 1269
Eco47III	AGCGCT	1 cut(s)	472
Eco53kI	GAGCTC	1 cut(s)	337
Eco57I	CTGAAG	1 cut(s)	1395
Eco88I	CYCGRG	1 cut(s)	410
EcoICRI	GAGCTC	1 cut(s)	337
EcoRII	CCWGG	2 cut(s)	276, 1227
EcoT22I	ATGCAT	1 cut(s)	6
EcoT38I	GRGCYC	2 cut(s)	339, 1572
Esp3I	CGTCTC	1 cut(s)	816
FaeI	CATG	6 cut(s)	692, 734, 749, 1306, 1438, 1445
FalI	AAGNNNNNCTT	2 cut(s)	1603, 1635
FaqI	GGGAC	1 cut(s)	121
FatI	CATG	6 cut(s)	688, 730, 745, 1302, 1434, 1441
FauI	CCCGC	2 cut(s)	261, 302
FbaI	TGATCA	2 cut(s)	697, 1243
FblI	GTMKAC	3 cut(s)	356, 1106, 1513
Fnu4HI	GCNGC	2 cut(s)	98, 400
FokI	GGATG	6 cut(s)	284, 891, 1007, 1052, 1087, 1187
FriOI	GRGCYC	2 cut(s)	339, 1572
Fsp4HI	GCNGC	2 cut(s)	98, 400
FspBI	CTAG	2 cut(s)	1281, 1469
GlaI	GCGC	1 cut(s)	472
GluI	GCNGC	2 cut(s)	98, 400
HaeII	RGCGCY	1 cut(s)	474
HaeIII	GGCC	1 cut(s)	579
HapII	CCGG	7 cut(s)	25, 43, 455, 581, 992, 1202, 1552
HgaI	GACGC	1 cut(s)	644
HhaI	GCGC	1 cut(s)	473
Hin1I	GRCGYC	1 cut(s)	636
Hin1II	CATG	6 cut(s)	692, 734, 749, 1306, 1438, 1445
Hin6I	GCGC	1 cut(s)	471
HinP1I	GCGC	1 cut(s)	471
HincII	GTYRAC	1 cut(s)	943
HindII	GTYRAC	1 cut(s)	943
HindIII	AAGCTT	1 cut(s)	1662
HinfI	GANTC	5 cut(s)	429, 557, 833, 1438, 1634
HpaII	CCGG	7 cut(s)	25, 43, 455, 581, 992, 1202, 1552
HphI	GGTGA	7 cut(s)	118, 189, 209, 232, 261, 314, 842
Hpy166II	GTNNAC	5 cut(s)	357, 943, 1107, 1466, 1514
Hpy188I	TCNGA	3 cut(s)	39, 393, 838
Hpy8I	GTNNAC	5 cut(s)	357, 943, 1107, 1466, 1514
Hpy99I	CGWCG	1 cut(s)	638
HpyAV	CCTTC	4 cut(s)	179, 238, 1422, 1502
HpyCH4III	ACNGT	2 cut(s)	88, 1534
HpyCH4IV	ACGT	4 cut(s)	825, 847, 1208, 1462
HpyCH4V	TGCA	8 cut(s)	4, 33, 402, 422, 1223, 1302, 1452, 1595
HpyF10VI	GCNNNNNNNGC	8 cut(s)	30, 303, 477, 644, 1229, 1339, 1620, 1670
HpyF3I	CTNAG	5 cut(s)	140, 302, 1268, 1544, 1564
HpySE526I	ACGT	4 cut(s)	825, 847, 1208, 1462
Hsp92I	GRCGYC	1 cut(s)	636
Hsp92II	CATG	6 cut(s)	692, 734, 749, 1306, 1438, 1445
HspAI	GCGC	1 cut(s)	471
Ksp22I	TGATCA	2 cut(s)	697, 1243
Kzo9I	GATC	8 cut(s)	39, 52, 143, 691, 697, 1243, 1309, 1500
LmnI	GCTCC	2 cut(s)	334, 1575
Lsp1109I	GCAGC	2 cut(s)	84, 386
LweI	GCATC	4 cut(s)	306, 985, 1530, 1682
MabI	ACCWGGT	1 cut(s)	276
MaeI	CTAG	2 cut(s)	1281, 1469
MaeII	ACGT	4 cut(s)	825, 847, 1208, 1462
MaeIII	GTNAC	5 cut(s)	88, 1214, 1547, 1691, 1703
MalI	GATC	8 cut(s)	41, 54, 145, 693, 699, 1245, 1311, 1502
MboI	GATC	8 cut(s)	39, 52, 143, 691, 697, 1243, 1309, 1500
MfeI	CAATTG	1 cut(s)	1114
MflI	RGATCY	1 cut(s)	143
MhlI	GDGCHC	2 cut(s)	339, 1572
MluCI	AATT	7 cut(s)	450, 585, 622, 672, 749, 1114, 1130
MlyI	GAGTC	2 cut(s)	566, 1643
Mph1103I	ATGCAT	1 cut(s)	6
MseI	TTAA	5 cut(s)	650, 675, 765, 1257, 1335
MslI	CAYNNNNRTG	2 cut(s)	131, 548
MspCI	CTTAAG	1 cut(s)	1334
MspI	CCGG	7 cut(s)	25, 43, 455, 581, 992, 1202, 1552
MspR9I	CCNGG	5 cut(s)	26, 278, 456, 581, 1229
MunI	CAATTG	1 cut(s)	1114
Mva1269I	GAATGC	1 cut(s)	790
MvaI	CCWGG	2 cut(s)	278, 1229
MvnI	CGCG	1 cut(s)	633
MwoI	GCNNNNNNNGC	8 cut(s)	30, 303, 477, 644, 1229, 1339, 1620, 1670
NciI	CCSGG	3 cut(s)	26, 456, 581
NdeII	GATC	8 cut(s)	39, 52, 143, 691, 697, 1243, 1309, 1500
NlaIII	CATG	6 cut(s)	692, 734, 749, 1306, 1438, 1445
NlaIV	GGNNCC	2 cut(s)	317, 1571
NmuCI	GTSAC	3 cut(s)	88, 1691, 1703
NruI	TCGCGA	1 cut(s)	633
NsiI	ATGCAT	1 cut(s)	6
NspI	RCATGY	2 cut(s)	734, 1306
PaeI	GCATGC	1 cut(s)	1306
PagI	TCATGA	2 cut(s)	688, 1434
PctI	GAATGC	1 cut(s)	790
PfeI	GAWTC	3 cut(s)	429, 833, 1438
PflMI	CCANNNNNTGG	2 cut(s)	331, 1171
PkrI	GCNGC	2 cut(s)	99, 401
Ple19I	CGATCG	1 cut(s)	1312
PleI	GAGTC	2 cut(s)	565, 1642
PpsI	GAGTC	2 cut(s)	565, 1642
Ppu21I	YACGTR	2 cut(s)	848, 1209
Psp124BI	GAGCTC	1 cut(s)	339
Psp6I	CCWGG	2 cut(s)	276, 1227
PspGI	CCWGG	2 cut(s)	276, 1227
PspN4I	GGNNCC	2 cut(s)	317, 1571
PspPI	GGNCC	1 cut(s)	578
PsuI	RGATCY	1 cut(s)	143
PvuI	CGATCG	1 cut(s)	1312
RruI	TCGCGA	1 cut(s)	633
RsaI	GTAC	1 cut(s)	1003
RsaNI	GTAC	1 cut(s)	1002
RseI	CAYNNNNRTG	2 cut(s)	131, 548
SacI	GAGCTC	1 cut(s)	339
SaqAI	TTAA	5 cut(s)	650, 675, 765, 1257, 1335
SatI	GCNGC	2 cut(s)	98, 400
Sau3AI	GATC	8 cut(s)	39, 52, 143, 691, 697, 1243, 1309, 1500
Sau96I	GGNCC	1 cut(s)	578
SchI	GAGTC	2 cut(s)	566, 1643
ScrFI	CCNGG	5 cut(s)	26, 278, 456, 581, 1229
SduI	GDGCHC	2 cut(s)	339, 1572
SexAI	ACCWGGT	1 cut(s)	276
SfaNI	GCATC	4 cut(s)	306, 985, 1530, 1682
SmiMI	CAYNNNNRTG	2 cut(s)	131, 548
SmlI	CTYRAG	1 cut(s)	1334
SmoI	CTYRAG	1 cut(s)	1334
SphI	GCATGC	1 cut(s)	1306
Sse9I	AATT	7 cut(s)	450, 585, 622, 672, 749, 1114, 1130
SsiI	CCGC	5 cut(s)	220, 254, 272, 295, 1517
SspMI	CTAG	2 cut(s)	1281, 1469
SstI	GAGCTC	1 cut(s)	339
StyD4I	CCNGG	5 cut(s)	24, 276, 454, 579, 1227
TaaI	ACNGT	2 cut(s)	88, 1534
TaiI	ACGT	4 cut(s)	828, 850, 1211, 1465
TaqI	TCGA	9 cut(s)	51, 463, 643, 655, 977, 1010, 1312, 1503, 1575
TasI	AATT	7 cut(s)	450, 585, 622, 672, 749, 1114, 1130
TfiI	GAWTC	3 cut(s)	429, 833, 1438
Tru1I	TTAA	5 cut(s)	650, 675, 765, 1257, 1335
Tru9I	TTAA	5 cut(s)	650, 675, 765, 1257, 1335
TscAI	CASTG	4 cut(s)	93, 99, 688, 1253
TseFI	GTSAC	3 cut(s)	88, 1691, 1703
TseI	GCWGC	2 cut(s)	97, 399
Tsp45I	GTSAC	3 cut(s)	88, 1691, 1703
TspDTI	ATGAA	8 cut(s)	677, 762, 876, 1006, 1037, 1191, 1430, 1521
TspRI	CASTG	4 cut(s)	93, 99, 688, 1253
Van91I	CCANNNNNTGG	2 cut(s)	331, 1171
Vha464I	CTTAAG	1 cut(s)	1334
XapI	RAATTY	2 cut(s)	585, 749
XbaI	TCTAGA	1 cut(s)	1280
XceI	RCATGY	2 cut(s)	734, 1306
XmiI	GTMKAC	3 cut(s)	356, 1106, 1513
XspI	CTAG	2 cut(s)	1281, 1469
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.