Rorug02G0328900

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0006290
Physical Location & Seq
Forward (+)
40344510 .. 40345184
675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0328900.1

Sequence Viewer

Length: 675 bp
ATGGCTACACTCCAAGAAACACATCATCCACTTTTGGAGAATTATAGTAGTAGTAATAATTCACCACCGACAACAGCATCTGCGTCATCACCATTACCGTCATCGTCAATAGTAGCTTCAAAACCATCAAAAACACCAGCAGAAAAGGCAATGAGAAAGACATTCAAAGGCACAGCCCATTTGGCCAATCTTCTCCCAACTGGAACAGTTCTCACATTCCAAATGTTCTCTCCAGCTTTTACACACCAAGGCAAGTGCCCCACCGTTGCCAACCATACGATGACATTATCCCTTCTAATATTTTGCTCTGTCTCCTCTTTTCTTTTGTGCTTCACCGATAGCTTCCGCGACGGGCGAGGAAAAGTTCGATATGGGTTGGCCACTTTCAAAGGATTGTTTGTGCTTGATGGGTCTCACACGATTGGGGCAGAGGAGGCAGCAAAATACAAGCTGACATTCATTGATTTCTTCCATGCTTTTATGTCCGTGTTGGTGTTTGGGGCGGTGGCATCGTTTGATCAGAATATTGTCAATTGCTTTTATCCCAAGCCATCAGAGGAGGGGAAACAACTTTTGTTTGCGGTGCCGGTTGGAGTTGGTGTTGTTTGCAGTATCTTATTTGTTTTGTTTCCTACTAGGCGCCATGGAATTGGCTTCCCTCTTTCTCGTAGTTAG

Protein Analysis

224

Amino Acids

24.25

Weight (kDa)

9.3

Isoelectric Point (pI)

44.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 57 - 219 1.2e-67 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 583, 639
AccII CGCG 1 cut(s) 348
AciI CCGC 3 cut(s) 346, 503, 581
AcoI YGGCCR 2 cut(s) 183, 378
AcyI GRCGYC 1 cut(s) 640
AgsI TTSAA 3 cut(s) 120, 166, 388
AluBI AGCT 4 cut(s) 116, 236, 342, 451
AluI AGCT 4 cut(s) 116, 236, 342, 451
Alw26I GTCTC 2 cut(s) 316, 417
AlwNI CAGNNNCTG 1 cut(s) 80
AoxI GGCC 2 cut(s) 183, 378
ApeKI GCWGC 1 cut(s) 437
AspLEI GCGC 1 cut(s) 642
AsuHPI GGTGA 3 cut(s) 54, 81, 325
BaeGI GKGCMC 1 cut(s) 260
BalI TGGCCA 2 cut(s) 185, 380
BanI GGYRCC 2 cut(s) 583, 639
BbvI GCAGC 1 cut(s) 449
BccI CCATC 3 cut(s) 133, 401, 559
BclI TGATCA 1 cut(s) 517
BcoDI GTCTC 2 cut(s) 316, 417
BfaI CTAG 1 cut(s) 636
BfoI RGCGCY 1 cut(s) 643
BglI GCCNNNNNGGC 1 cut(s) 182
BisI GCNGC 1 cut(s) 438
BlsI GCNGC 1 cut(s) 439
BmiI GGNNCC 2 cut(s) 585, 641
BmsI GCATC 2 cut(s) 86, 518
BpmI CTGGAG 1 cut(s) 216
BsaHI GRCGYC 1 cut(s) 640
BsaI GGTCTC 1 cut(s) 417
BsaJI CCNNGG 2 cut(s) 247, 643
BsaXI ACNNNNNCTCC 2 cut(s) 585, 615
Bse118I RCCGGY 1 cut(s) 586
Bse1I ACTGG 1 cut(s) 205
Bse3DI GCAATG 1 cut(s) 156
BseDI CCNNGG 2 cut(s) 247, 643
BseGI GGATG 1 cut(s) 25
BseMI GCAATG 1 cut(s) 156
BseNI ACTGG 1 cut(s) 205
BseRI GAGGAG 3 cut(s) 304, 446, 572
BseSI GKGCMC 1 cut(s) 260
BseXI GCAGC 1 cut(s) 449
Bsh1236I CGCG 1 cut(s) 348
BshFI GGCC 2 cut(s) 185, 380
BshNI GGYRCC 2 cut(s) 583, 639
BsiSI CCGG 1 cut(s) 587
BsmAI GTCTC 2 cut(s) 316, 417
BsnI GGCC 2 cut(s) 185, 380
Bso31I GGTCTC 1 cut(s) 417
Bsp1286I GDGCHC 1 cut(s) 260
Bsp143I GATC 1 cut(s) 517
Bsp19I CCATGG 1 cut(s) 643
BspACI CCGC 3 cut(s) 346, 503, 581
BspANI GGCC 2 cut(s) 185, 380
BspFNI CGCG 1 cut(s) 348
BspLI GGNNCC 2 cut(s) 585, 641
BspT107I GGYRCC 2 cut(s) 583, 639
BspTNI GGTCTC 1 cut(s) 417
BsrDI GCAATG 1 cut(s) 156
BsrFI RCCGGY 1 cut(s) 586
BsrI ACTGG 1 cut(s) 205
BssAI RCCGGY 1 cut(s) 586
BssECI CCNNGG 2 cut(s) 247, 643
BssMI GATC 1 cut(s) 517
BssNI GRCGYC 1 cut(s) 640
BssT1I CCWWGG 2 cut(s) 247, 643
Bst4CI ACNGT 3 cut(s) 99, 208, 265
BstACI GRCGYC 1 cut(s) 640
BstDSI CCRYGG 1 cut(s) 643
BstF5I GGATG 1 cut(s) 25
BstFNI CGCG 1 cut(s) 348
BstH2I RGCGCY 1 cut(s) 643
BstHHI GCGC 1 cut(s) 642
BstKTI GATC 1 cut(s) 520
BstMAI GTCTC 2 cut(s) 316, 417
BstMBI GATC 1 cut(s) 517
BstMWI GCNNNNNNNGC 3 cut(s) 146, 182, 434
BstSLI GKGCMC 1 cut(s) 260
BstUI CGCG 1 cut(s) 348
BstV1I GCAGC 1 cut(s) 449
BstXI CCANNNNNNTGG 1 cut(s) 650
BsuRI GGCC 2 cut(s) 185, 380
BtgI CCRYGG 1 cut(s) 643
BtsCI GGATG 1 cut(s) 25
CaiI CAGNNNCTG 1 cut(s) 80
CfoI GCGC 1 cut(s) 642
Cfr10I RCCGGY 1 cut(s) 586
CseI GACGC 1 cut(s) 72
CviAII CATG 2 cut(s) 473, 644
DinI GGCGCC 1 cut(s) 641
DpnI GATC 1 cut(s) 519
DpnII GATC 1 cut(s) 517
EaeI YGGCCR 2 cut(s) 183, 378
Eco130I CCWWGG 2 cut(s) 247, 643
Eco31I GGTCTC 1 cut(s) 417
EcoT14I CCWWGG 2 cut(s) 247, 643
EgeI GGCGCC 1 cut(s) 641
EheI GGCGCC 1 cut(s) 641
ErhI CCWWGG 2 cut(s) 247, 643
FaeI CATG 2 cut(s) 476, 647
FaiI YATR 6 cut(s) 45, 276, 372, 474, 482, 645
FatI CATG 2 cut(s) 472, 643
FbaI TGATCA 1 cut(s) 517
Fnu4HI GCNGC 1 cut(s) 438
FokI GGATG 1 cut(s) 12
Fsp4HI GCNGC 1 cut(s) 438
FspBI CTAG 1 cut(s) 636
GlaI GCGC 1 cut(s) 641
GluI GCNGC 1 cut(s) 438
GsuI CTGGAG 1 cut(s) 216
HaeII RGCGCY 1 cut(s) 643
HaeIII GGCC 2 cut(s) 185, 380
HapII CCGG 1 cut(s) 587
HgaI GACGC 1 cut(s) 72
HhaI GCGC 1 cut(s) 642
Hin1I GRCGYC 1 cut(s) 640
Hin1II CATG 2 cut(s) 476, 647
Hin6I GCGC 1 cut(s) 640
HinP1I GCGC 1 cut(s) 640
HpaII CCGG 1 cut(s) 587
HphI GGTGA 3 cut(s) 54, 81, 325
Hpy188I TCNGA 2 cut(s) 522, 556
Hpy99I CGWCG 1 cut(s) 353
HpyAV CCTTC 1 cut(s) 302
HpyCH4III ACNGT 3 cut(s) 99, 208, 265
HpyCH4V TGCA 1 cut(s) 609
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 182, 434
Hsp92I GRCGYC 1 cut(s) 640
Hsp92II CATG 2 cut(s) 476, 647
HspAI GCGC 1 cut(s) 640
KasI GGCGCC 1 cut(s) 639
Ksp22I TGATCA 1 cut(s) 517
Kzo9I GATC 1 cut(s) 517
LpnPI CCDG 4 cut(s) 150, 186, 246, 600
Lsp1109I GCAGC 1 cut(s) 449
LweI GCATC 2 cut(s) 86, 518
MaeI CTAG 1 cut(s) 636
MalI GATC 1 cut(s) 519
MboI GATC 1 cut(s) 517
MboII GAAGA 2 cut(s) 182, 460
MfeI CAATTG 1 cut(s) 532
MhlI GDGCHC 1 cut(s) 260
MlsI TGGCCA 2 cut(s) 185, 380
MluCI AATT 4 cut(s) 40, 58, 532, 648
MluNI TGGCCA 2 cut(s) 185, 380
Mly113I GGCGCC 1 cut(s) 640
MmeI TCCRAC 1 cut(s) 571
MnlI CCTC 7 cut(s) 325, 350, 424, 427, 550, 553, 669
Mox20I TGGCCA 2 cut(s) 185, 380
MscI TGGCCA 2 cut(s) 185, 380
Msp20I TGGCCA 2 cut(s) 185, 380
MspI CCGG 1 cut(s) 587
MunI CAATTG 1 cut(s) 532
MvnI CGCG 1 cut(s) 348
MwoI GCNNNNNNNGC 3 cut(s) 146, 182, 434
NarI GGCGCC 1 cut(s) 640
NcoI CCATGG 1 cut(s) 643
NdeII GATC 1 cut(s) 517
NlaIII CATG 2 cut(s) 476, 647
NlaIV GGNNCC 2 cut(s) 585, 641
PkrI GCNGC 1 cut(s) 439
PluTI GGCGCC 1 cut(s) 643
PspN4I GGNNCC 2 cut(s) 585, 641
PstNI CAGNNNCTG 1 cut(s) 80
SatI GCNGC 1 cut(s) 438
Sau3AI GATC 1 cut(s) 517
SduI GDGCHC 1 cut(s) 260
SetI ASST 4 cut(s) 118, 238, 344, 453
SfaNI GCATC 2 cut(s) 86, 518
SfoI GGCGCC 1 cut(s) 641
Sse9I AATT 4 cut(s) 40, 58, 532, 648
SsiI CCGC 3 cut(s) 346, 503, 581
SspDI GGCGCC 1 cut(s) 639
SspI AATATT 2 cut(s) 300, 526
SspMI CTAG 1 cut(s) 636
StyI CCWWGG 2 cut(s) 247, 643
TaaI ACNGT 3 cut(s) 99, 208, 265
TaqI TCGA 1 cut(s) 367
TasI AATT 4 cut(s) 40, 58, 532, 648
TseI GCWGC 1 cut(s) 437
TspDTI ATGAA 1 cut(s) 448
TspGWI ACGGA 1 cut(s) 475
XspI CTAG 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.