pycom17g22830

Chaperone protein dnaJ 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0013509
Physical Location & Seq
Reverse (-)
21225261 .. 21227119
1859 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 483 bp
ATGGCTTGCGTTGGAGCTGGTTTGATCGGGGGCAGTGGGTCGTCCTGGAATCTGTTGAAGGATAATAAGGCGAGGAGGAAGAAGATGGTGAATGATAAGAGGGGGATGGTTTCTTGTGTTGCTTCTTCTTCTTCTGTGATGGATCCTTACCGGACCTTGAAGATTCAACCTGGTGCCTCTGAATCTGAGGTCAGGAAGGCTTTCAGACAACTTGCTTTGAAGTATCATCCAGATGTATGCAGAGGAAACAATTGTAGAGTTCAGTTTCACCAGATCAATGAAGCCTATGATATTGTCATGAGCGATTTGAGAGGAGAAACGACGACGACTACTCCATCGCAGATGTATGAGACGTACGACGAAGGGGATGACGAGCCGATGAGGGGAATGAACGATCCAGATTGGGACATGTGGGAGGAATGGATGGGATGGGAAGGAGCTGGAATTAGGGACTACACATCTCACATTAATCCTTATATTTGA

Protein Analysis

161

Amino Acids

18.14

Weight (kDa)

5.38

Isoelectric Point (pI)

41.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 48 - 100 1.9e-15 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013509)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80920
fragaria_vesca FvH4_6g28480
malus_domestica MD09G1225800.v1.1 MD17G1224600.v1.1
prunus_persica Prupe.3G100300_v2.0.a1
pyrus_communis pycom09g14530 pycom17g22830
rosa_chinensis RchiOBHm_Chr2g0132701
rosa_laevigata RLG00000019273
rosa_multiflora Rmu_ssc0000074.1_g000011
rosa_roxburghii Rroxscaffold_2G00111720
rosa_rugosa Rorug02G0306300
rosa_samantha Rh2AG359400 Rh2BG365700 Rh2CG342500 Rh2DG382400
rosa_wichuraiana Rw2G029240 Rw3G024410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 173
AclWI GGATC 3 cut(s) 137, 150, 389
AfaI GTAC 1 cut(s) 356
AfiI CCNNNNNNNGG 1 cut(s) 383
AflIII ACRYGT 1 cut(s) 408
AgsI TTSAA 4 cut(s) 58, 160, 167, 220
AjnI CCWGG 2 cut(s) 44, 169
AluBI AGCT 2 cut(s) 17, 440
AluI AGCT 2 cut(s) 17, 440
Alw26I GTCTC 1 cut(s) 344
AlwI GGATC 3 cut(s) 137, 150, 389
AseI ATTAAT 1 cut(s) 468
Asp700I GAANNNNTTC 1 cut(s) 200
AspS9I GGNCC 1 cut(s) 153
AsuHPI GGTGA 2 cut(s) 100, 260
AvaII GGWCC 1 cut(s) 153
BamHI GGATCC 1 cut(s) 142
BanI GGYRCC 1 cut(s) 173
BccI CCATC 6 cut(s) 79, 100, 133, 343, 418, 423
BciT130I CCWGG 2 cut(s) 46, 171
BcoDI GTCTC 1 cut(s) 344
Bme1390I CCNGG 2 cut(s) 46, 171
Bme18I GGWCC 1 cut(s) 153
BmgT120I GGNCC 1 cut(s) 153
BmiI GGNNCC 2 cut(s) 144, 175
BmrFI CCNGG 2 cut(s) 46, 171
BsaWI WCCGGW 1 cut(s) 150
BsaXI ACNNNNNCTCC 2 cut(s) 316, 346
Bsc4I CCNNNNNNNGG 1 cut(s) 383
BseBI CCWGG 2 cut(s) 46, 171
BseGI GGATG 5 cut(s) 111, 226, 373, 429, 434
BseLI CCNNNNNNNGG 1 cut(s) 383
BseMII CTCAG 1 cut(s) 177
BseRI GAGGAG 2 cut(s) 88, 327
BshNI GGYRCC 1 cut(s) 173
BsiSI CCGG 1 cut(s) 151
BsiWI CGTACG 1 cut(s) 354
BslFI GGGAC 2 cut(s) 419, 464
BslI CCNNNNNNNGG 1 cut(s) 383
BsmAI GTCTC 1 cut(s) 344
BsmBI CGTCTC 1 cut(s) 344
BsmFI GGGAC 2 cut(s) 419, 464
Bsp143I GATC 4 cut(s) 24, 142, 273, 394
BspCNI CTCAG 1 cut(s) 178
BspHI TCATGA 1 cut(s) 297
BspLI GGNNCC 2 cut(s) 144, 175
BspPI GGATC 3 cut(s) 137, 150, 389
BspT107I GGYRCC 1 cut(s) 173
BssMI GATC 4 cut(s) 24, 142, 273, 394
Bst2UI CCWGG 2 cut(s) 46, 171
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 1 cut(s) 186
BstF5I GGATG 5 cut(s) 111, 226, 373, 429, 434
BstKTI GATC 4 cut(s) 27, 145, 276, 397
BstMAI GTCTC 1 cut(s) 344
BstMBI GATC 4 cut(s) 24, 142, 273, 394
BstNI CCWGG 2 cut(s) 46, 171
BstNSI RCATGY 1 cut(s) 412
BstSCI CCNGG 2 cut(s) 44, 169
BstX2I RGATCY 1 cut(s) 142
BstYI RGATCY 1 cut(s) 142
BtgZI GCGATG 1 cut(s) 321
BtsCI GGATG 5 cut(s) 111, 226, 373, 429, 434
BtsI GCAGTG 1 cut(s) 40
BtsIMutI CAGTG 1 cut(s) 40
Cac8I GCNNGC 1 cut(s) 7
CciI TCATGA 1 cut(s) 297
Cfr13I GGNCC 1 cut(s) 153
CsiI ACCWGGT 1 cut(s) 169
Csp6I GTAC 1 cut(s) 355
CviAII CATG 2 cut(s) 298, 409
CviJI RGCY 6 cut(s) 5, 17, 200, 284, 376, 440
CviKI_1 RGCY 6 cut(s) 5, 17, 200, 284, 376, 440
CviQI GTAC 1 cut(s) 355
DdeI CTNAG 1 cut(s) 186
DpnI GATC 4 cut(s) 26, 144, 275, 396
DpnII GATC 4 cut(s) 24, 142, 273, 394
Eco47I GGWCC 1 cut(s) 153
EcoRII CCWGG 2 cut(s) 44, 169
Esp3I CGTCTC 1 cut(s) 344
FaeI CATG 2 cut(s) 301, 412
FaiI YATR 6 cut(s) 238, 288, 299, 348, 410, 477
FaqI GGGAC 2 cut(s) 419, 464
FatI CATG 2 cut(s) 297, 408
FokI GGATG 5 cut(s) 118, 213, 380, 436, 441
HapII CCGG 1 cut(s) 151
Hin1II CATG 2 cut(s) 301, 412
HinfI GANTC 3 cut(s) 49, 163, 182
HpaII CCGG 1 cut(s) 151
HphI GGTGA 2 cut(s) 100, 260
Hpy188I TCNGA 3 cut(s) 181, 187, 206
Hpy188III TCNNGA 4 cut(s) 193, 230, 298, 398
Hpy99I CGWCG 3 cut(s) 325, 328, 362
HpyAV CCTTC 4 cut(s) 52, 190, 356, 428
HpyCH4IV ACGT 1 cut(s) 353
HpyCH4V TGCA 1 cut(s) 240
HpyF3I CTNAG 1 cut(s) 186
HpySE526I ACGT 1 cut(s) 353
Hsp92II CATG 2 cut(s) 301, 412
Kzo9I GATC 4 cut(s) 24, 142, 273, 394
LmnI GCTCC 2 cut(s) 14, 437
MabI ACCWGGT 1 cut(s) 169
MaeII ACGT 1 cut(s) 353
MalI GATC 4 cut(s) 26, 144, 275, 396
MboI GATC 4 cut(s) 24, 142, 273, 394
MboII GAAGA 6 cut(s) 91, 94, 117, 120, 123, 172
MfeI CAATTG 1 cut(s) 250
MflI RGATCY 1 cut(s) 142
MluCI AATT 2 cut(s) 250, 444
MnlI CCTC 9 cut(s) 66, 69, 93, 181, 187, 236, 305, 375, 409
MroXI GAANNNNTTC 1 cut(s) 200
MseI TTAA 1 cut(s) 468
MslI CAYNNNNRTG 1 cut(s) 231
MspI CCGG 1 cut(s) 151
MspR9I CCNGG 2 cut(s) 46, 171
MunI CAATTG 1 cut(s) 250
MvaI CCWGG 2 cut(s) 46, 171
NdeII GATC 4 cut(s) 24, 142, 273, 394
NlaIII CATG 2 cut(s) 301, 412
NlaIV GGNNCC 2 cut(s) 144, 175
NspI RCATGY 1 cut(s) 412
PagI TCATGA 1 cut(s) 297
PciI ACATGT 1 cut(s) 408
PdmI GAANNNNTTC 1 cut(s) 200
PfeI GAWTC 3 cut(s) 49, 163, 182
Pfl23II CGTACG 1 cut(s) 354
PfoI TCCNGGA 1 cut(s) 44
PscI ACATGT 1 cut(s) 408
PshBI ATTAAT 1 cut(s) 468
Psp6I CCWGG 2 cut(s) 44, 169
PspGI CCWGG 2 cut(s) 44, 169
PspLI CGTACG 1 cut(s) 354
PspN4I GGNNCC 2 cut(s) 144, 175
PspPI GGNCC 1 cut(s) 153
PsuI RGATCY 1 cut(s) 142
RsaI GTAC 1 cut(s) 356
RsaNI GTAC 1 cut(s) 355
RseI CAYNNNNRTG 1 cut(s) 231
SaqAI TTAA 1 cut(s) 468
Sau3AI GATC 4 cut(s) 24, 142, 273, 394
Sau96I GGNCC 1 cut(s) 153
ScrFI CCNGG 2 cut(s) 46, 171
SetI ASST 6 cut(s) 19, 158, 172, 192, 356, 442
SexAI ACCWGGT 1 cut(s) 169
SinI GGWCC 1 cut(s) 153
SmiMI CAYNNNNRTG 1 cut(s) 231
Sse9I AATT 2 cut(s) 250, 444
StyD4I CCNGG 2 cut(s) 44, 169
TaiI ACGT 1 cut(s) 356
TasI AATT 2 cut(s) 250, 444
TfiI GAWTC 3 cut(s) 49, 163, 182
Tru1I TTAA 1 cut(s) 468
Tru9I TTAA 1 cut(s) 468
TscAI CASTG 1 cut(s) 40
TspDTI ATGAA 2 cut(s) 294, 404
TspRI CASTG 1 cut(s) 40
VpaK11BI GGWCC 1 cut(s) 153
VspI ATTAAT 1 cut(s) 468
XceI RCATGY 1 cut(s) 412
XmnI GAANNNNTTC 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.