pycom17g24890

Alpha beta hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0010688
Physical Location & Seq
Reverse (-)
23116282 .. 23119766
3485 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1026 bp
ATGGGCGGAGTGACCTCTTCGATCGCAGCCAAGTTCGCTTTTTTCCCGCCGAGTCCGCCGTCGTACACAGTCGTCGCCGACGAGTCGTGTGAAGGGAGGTTCCTCATTCCGGAGGTACCGAGGAGGGACGACGTGGATGTGCTGAAGCTCAGGAATCGCCGGGGGAATGAAATCGTGGCCGTACACGTCAAGCACCCCAAGGCCTCTGCAACTATGCTTTACTCCCATGGAAATGCTGCTGATTTGGGGCAGATGTTTGAGCTTTTCGTCGAATTGAGTACTCGCCTTCGGATTAATTTGATGGGGTATGATTACTCTGGTTATGGACAGTCAACTGGAAAGGCAACTGAGTGTAATACGTATGCGGACATTGATGCAGCTTATAAATGCCTCAAGGAGAAATACGGGGTCAAAGATGAACAACTAATATTATATGGTCAGTCTGTTGGTAGTGGTCCCACTGTCGATCTTGCTTCACGTCTACCAACCTTGAGAGGTGTGGTATTACACAGTCCCATTTTGTCTGGGCTGAGGGTGTTGTACCCTGTCAAGCGGACATACTGGTTTGATATTTACAAGAATATCGACAAAATTTCAATGGTGAACTGTCCTGTCCTGGTTATACATGGGACAGCAGACGAGGTTGTAGATTCGTCCCATGGGAAGCAGCTTTGGGTACTTTGCAAGGAAAAGTACGACCCCTTATGGCTCAGTGGAGGTGGGCACGGCAATCTTGAGCTTTACCCAGAATTTATCAAACATCTAAAGAAATTTGTACACAGCCTTGGCAAGTCAAAATCGACCACAAATGGTTCTAGAACAAGTACAGTAGATTCTGCGAACGAGAACAAACCATCTGAAACCGGTACTTCAGATTCATGCGAAGGAGGTTCTGATCGTCCAGAAGTTTCTAGAAACAGTTTAGATAGTCGACTAGAGAAGTCTAAAAAGTCGAACAAGCCCGAAAAGTCTCGGATGAGCACTGACCGAGCCGACACGTTTAGGAGAAAGAAGGGTTTAGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FSH1 Hydrolase_4

Protein Analysis

342

Amino Acids

37.71

Weight (kDa)

9.02

Isoelectric Point (pI)

43.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 67 - 175 8.1e-09 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 384
Acc65I GGTACC 1 cut(s) 115
AccB1I GGYRCC 1 cut(s) 115
AccI GTMKAC 2 cut(s) 481, 931
AccIII TCCGGA 1 cut(s) 109
AciI CCGC 5 cut(s) 6, 47, 56, 365, 553
AcoI YGGCCR 1 cut(s) 177
AcsI RAATTY 3 cut(s) 591, 749, 770
AcuI CTGAAG 2 cut(s) 164, 855
AfiI CCNNNNNNNGG 1 cut(s) 109
AflIII ACRYGT 2 cut(s) 184, 996
AgeI ACCGGT 1 cut(s) 863
AgsI TTSAA 1 cut(s) 597
AjiI CACGTC 3 cut(s) 133, 187, 479
AjnI CCWGG 1 cut(s) 615
AluBI AGCT 5 cut(s) 148, 262, 380, 670, 739
AluI AGCT 5 cut(s) 148, 262, 380, 670, 739
Alw21I GWGCWC 1 cut(s) 983
Alw26I GTCTC 1 cut(s) 975
Aor13HI TCCGGA 1 cut(s) 109
AoxI GGCC 2 cut(s) 177, 201
ApeKI GCWGC 4 cut(s) 26, 236, 377, 667
ApoI RAATTY 3 cut(s) 591, 749, 770
AseI ATTAAT 1 cut(s) 294
AsiGI ACCGGT 1 cut(s) 863
Asp718I GGTACC 1 cut(s) 115
AspS9I GGNCC 1 cut(s) 455
AsuC2I CCSGG 1 cut(s) 161
AsuHPI GGTGA 1 cut(s) 613
AvaII GGWCC 1 cut(s) 455
BaeGI GKGCMC 1 cut(s) 726
BanI GGYRCC 1 cut(s) 115
Bbv12I GWGCWC 1 cut(s) 983
BbvCI CCTCAGC 1 cut(s) 530
BbvI GCAGC 4 cut(s) 38, 223, 389, 679
BccI CCATC 2 cut(s) 295, 862
BceAI ACGGC 3 cut(s) 43, 164, 742
BciT130I CCWGG 1 cut(s) 617
BcnI CCSGG 1 cut(s) 161
BcoDI GTCTC 1 cut(s) 975
BfaI CTAG 3 cut(s) 816, 912, 935
BisI GCNGC 4 cut(s) 27, 237, 378, 668
BlsI GCNGC 4 cut(s) 28, 238, 379, 669
BmcAI AGTACT 1 cut(s) 280
Bme1390I CCNGG 2 cut(s) 161, 617
Bme18I GGWCC 1 cut(s) 455
BmgBI CACGTC 3 cut(s) 133, 187, 479
BmgT120I GGNCC 1 cut(s) 455
BmiI GGNNCC 3 cut(s) 101, 117, 457
BmrFI CCNGG 2 cut(s) 161, 617
BmsI GCATC 1 cut(s) 364
Bpu10I CCTNAGC 2 cut(s) 149, 530
BpuEI CTTGAG 3 cut(s) 377, 511, 755
BpuMI CCSGG 1 cut(s) 161
BsaAI YACGTR 1 cut(s) 360
BsaJI CCNNGG 6 cut(s) 119, 160, 198, 226, 658, 784
BsaWI WCCGGW 2 cut(s) 109, 863
Bsc4I CCNNNNNNNGG 1 cut(s) 109
Bse118I RCCGGY 1 cut(s) 863
Bse1I ACTGG 2 cut(s) 340, 566
BseAI TCCGGA 1 cut(s) 109
BseBI CCWGG 1 cut(s) 617
BseDI CCNNGG 6 cut(s) 119, 160, 198, 226, 658, 784
BseGI GGATG 2 cut(s) 142, 981
BseLI CCNNNNNNNGG 1 cut(s) 109
BseMII CTCAG 4 cut(s) 163, 339, 521, 724
BseNI ACTGG 2 cut(s) 340, 566
BseRI GAGGAG 1 cut(s) 136
BseSI GKGCMC 1 cut(s) 726
BseXI GCAGC 4 cut(s) 38, 223, 389, 679
Bsh1285I CGRYCG 1 cut(s) 24
BshFI GGCC 2 cut(s) 179, 203
BshNI GGYRCC 1 cut(s) 115
BshTI ACCGGT 1 cut(s) 863
BsiEI CGRYCG 1 cut(s) 24
BsiHKAI GWGCWC 1 cut(s) 983
BsiSI CCGG 3 cut(s) 110, 160, 864
BslFI GGGAC 5 cut(s) 140, 441, 498, 640, 643
BslI CCNNNNNNNGG 1 cut(s) 109
BsmAI GTCTC 1 cut(s) 975
BsmFI GGGAC 5 cut(s) 140, 441, 498, 640, 643
BsnI GGCC 2 cut(s) 179, 203
Bsp1286I GDGCHC 2 cut(s) 726, 983
Bsp13I TCCGGA 1 cut(s) 109
Bsp1407I TGTACA 1 cut(s) 775
Bsp143I GATC 3 cut(s) 21, 466, 895
Bsp19I CCATGG 2 cut(s) 226, 658
BspACI CCGC 5 cut(s) 6, 47, 56, 365, 553
BspANI GGCC 2 cut(s) 179, 203
BspCNI CTCAG 4 cut(s) 162, 340, 522, 723
BspEI TCCGGA 1 cut(s) 109
BspLI GGNNCC 3 cut(s) 101, 117, 457
BspT107I GGYRCC 1 cut(s) 115
BsrFI RCCGGY 1 cut(s) 863
BsrGI TGTACA 1 cut(s) 775
BsrI ACTGG 2 cut(s) 340, 566
BssAI RCCGGY 1 cut(s) 863
BssECI CCNNGG 6 cut(s) 119, 160, 198, 226, 658, 784
BssMI GATC 3 cut(s) 21, 466, 895
BssT1I CCWWGG 4 cut(s) 198, 226, 658, 784
Bst2UI CCWGG 1 cut(s) 617
Bst4CI ACNGT 7 cut(s) 70, 330, 463, 512, 608, 829, 920
Bst6I CTCTTC 1 cut(s) 22
BstAUI TGTACA 1 cut(s) 775
BstBAI YACGTR 1 cut(s) 360
BstDEI CTNAG 4 cut(s) 149, 348, 530, 710
BstDSI CCRYGG 2 cut(s) 226, 658
BstF5I GGATG 2 cut(s) 142, 981
BstKTI GATC 3 cut(s) 24, 469, 898
BstMAI GTCTC 1 cut(s) 975
BstMBI GATC 3 cut(s) 21, 466, 895
BstMCI CGRYCG 1 cut(s) 24
BstMWI GCNNNNNNNGC 2 cut(s) 35, 55
BstNI CCWGG 1 cut(s) 617
BstSCI CCNGG 2 cut(s) 159, 615
BstSLI GKGCMC 1 cut(s) 726
BstSNI TACGTA 1 cut(s) 360
BstV1I GCAGC 4 cut(s) 38, 223, 389, 679
BsuRI GGCC 2 cut(s) 179, 203
BtgI CCRYGG 2 cut(s) 226, 658
BtrI CACGTC 3 cut(s) 133, 187, 479
BtsCI GGATG 2 cut(s) 142, 981
BtsIMutI CAGTG 3 cut(s) 459, 718, 981
Cfr10I RCCGGY 1 cut(s) 863
Cfr13I GGNCC 1 cut(s) 455
CspAI ACCGGT 1 cut(s) 863
CviAII CATG 4 cut(s) 227, 626, 659, 879
DdeI CTNAG 4 cut(s) 149, 348, 530, 710
DpnI GATC 3 cut(s) 23, 468, 897
DpnII GATC 3 cut(s) 21, 466, 895
EaeI YGGCCR 1 cut(s) 177
Eam1104I CTCTTC 1 cut(s) 22
EarI CTCTTC 1 cut(s) 22
EciI GGCGGA 2 cut(s) 21, 45
Eco105I TACGTA 1 cut(s) 360
Eco130I CCWWGG 4 cut(s) 198, 226, 658, 784
Eco147I AGGCCT 1 cut(s) 203
Eco47I GGWCC 1 cut(s) 455
Eco57I CTGAAG 2 cut(s) 164, 855
EcoRII CCWGG 1 cut(s) 615
EcoT14I CCWWGG 4 cut(s) 198, 226, 658, 784
ErhI CCWWGG 4 cut(s) 198, 226, 658, 784
FaeI CATG 4 cut(s) 230, 629, 662, 882
FaqI GGGAC 5 cut(s) 140, 441, 498, 640, 643
FatI CATG 4 cut(s) 226, 625, 658, 878
FauI CCCGC 1 cut(s) 54
FblI GTMKAC 2 cut(s) 481, 931
Fnu4HI GCNGC 4 cut(s) 27, 237, 378, 668
FokI GGATG 2 cut(s) 149, 988
Fsp4HI GCNGC 4 cut(s) 27, 237, 378, 668
FspBI CTAG 3 cut(s) 816, 912, 935
GluI GCNGC 4 cut(s) 27, 237, 378, 668
HaeIII GGCC 2 cut(s) 179, 203
HapII CCGG 3 cut(s) 110, 160, 864
Hin1II CATG 4 cut(s) 230, 629, 662, 882
HincII GTYRAC 2 cut(s) 333, 932
HindII GTYRAC 2 cut(s) 333, 932
HinfI GANTC 6 cut(s) 52, 83, 154, 650, 833, 875
HpaII CCGG 3 cut(s) 110, 160, 864
HphI GGTGA 1 cut(s) 613
Hpy166II GTNNAC 7 cut(s) 66, 184, 333, 482, 604, 778, 932
Hpy188I TCNGA 5 cut(s) 291, 859, 874, 895, 975
Hpy188III TCNNGA 6 cut(s) 110, 151, 734, 816, 902, 912
Hpy8I GTNNAC 7 cut(s) 66, 184, 333, 482, 604, 778, 932
Hpy99I CGWCG 5 cut(s) 64, 77, 83, 134, 272
HpyAV CCTTC 4 cut(s) 86, 296, 878, 1006
HpyCH4III ACNGT 7 cut(s) 70, 330, 463, 512, 608, 829, 920
HpyCH4IV ACGT 5 cut(s) 132, 186, 359, 478, 998
HpyCH4V TGCA 3 cut(s) 209, 377, 684
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 55
HpyF3I CTNAG 4 cut(s) 149, 348, 530, 710
HpySE526I ACGT 5 cut(s) 132, 186, 359, 478, 998
Hsp92II CATG 4 cut(s) 230, 629, 662, 882
Kpn2I TCCGGA 1 cut(s) 109
KpnI GGTACC 1 cut(s) 119
Kzo9I GATC 3 cut(s) 21, 466, 895
Lsp1109I GCAGC 4 cut(s) 38, 223, 389, 679
LweI GCATC 1 cut(s) 364
MaeI CTAG 3 cut(s) 816, 912, 935
MaeII ACGT 5 cut(s) 132, 186, 359, 478, 998
MaeIII GTNAC 1 cut(s) 10
MalI GATC 3 cut(s) 23, 468, 897
MboI GATC 3 cut(s) 21, 466, 895
MboII GAAGA 1 cut(s) 9
MhlI GDGCHC 2 cut(s) 726, 983
MluCI AATT 5 cut(s) 272, 295, 591, 749, 770
MlyI GAGTC 2 cut(s) 61, 92
MroI TCCGGA 1 cut(s) 109
MseI TTAA 1 cut(s) 294
MslI CAYNNNNRTG 1 cut(s) 231
MspI CCGG 3 cut(s) 110, 160, 864
MspR9I CCNGG 2 cut(s) 161, 617
MvaI CCWGG 1 cut(s) 617
MwoI GCNNNNNNNGC 2 cut(s) 35, 55
NciI CCSGG 1 cut(s) 161
NcoI CCATGG 2 cut(s) 226, 658
NdeII GATC 3 cut(s) 21, 466, 895
NlaIII CATG 4 cut(s) 230, 629, 662, 882
NlaIV GGNNCC 3 cut(s) 101, 117, 457
NmeAIII GCCGAG 1 cut(s) 75
NmuCI GTSAC 1 cut(s) 10
PceI AGGCCT 1 cut(s) 203
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PfeI GAWTC 4 cut(s) 154, 650, 833, 875
PinAI ACCGGT 1 cut(s) 863
PkrI GCNGC 4 cut(s) 28, 238, 379, 669
Ple19I CGATCG 1 cut(s) 24
PleI GAGTC 2 cut(s) 60, 91
PpsI GAGTC 2 cut(s) 60, 91
Ppu21I YACGTR 1 cut(s) 360
PshBI ATTAAT 1 cut(s) 294
PsiI TTATAA 1 cut(s) 384
Psp6I CCWGG 1 cut(s) 615
PspGI CCWGG 1 cut(s) 615
PspN4I GGNNCC 3 cut(s) 101, 117, 457
PspPI GGNCC 1 cut(s) 455
PvuI CGATCG 1 cut(s) 24
RseI CAYNNNNRTG 1 cut(s) 231
SalI GTCGAC 1 cut(s) 930
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 4 cut(s) 27, 237, 378, 668
Sau3AI GATC 3 cut(s) 21, 466, 895
Sau96I GGNCC 1 cut(s) 455
ScaI AGTACT 1 cut(s) 280
SchI GAGTC 2 cut(s) 61, 92
ScrFI CCNGG 2 cut(s) 161, 617
SduI GDGCHC 2 cut(s) 726, 983
SfaNI GCATC 1 cut(s) 364
SinI GGWCC 1 cut(s) 455
SmiMI CAYNNNNRTG 1 cut(s) 231
SmlI CTYRAG 3 cut(s) 392, 490, 734
SmoI CTYRAG 3 cut(s) 392, 490, 734
SnaBI TACGTA 1 cut(s) 360
Sse9I AATT 5 cut(s) 272, 295, 591, 749, 770
SseBI AGGCCT 1 cut(s) 203
SsiI CCGC 5 cut(s) 6, 47, 56, 365, 553
SspI AATATT 1 cut(s) 429
SspMI CTAG 3 cut(s) 816, 912, 935
StuI AGGCCT 1 cut(s) 203
StyD4I CCNGG 2 cut(s) 159, 615
StyI CCWWGG 4 cut(s) 198, 226, 658, 784
TaaI ACNGT 7 cut(s) 70, 330, 463, 512, 608, 829, 920
TaiI ACGT 5 cut(s) 135, 189, 362, 481, 1001
TaqI TCGA 7 cut(s) 20, 270, 465, 585, 800, 931, 953
TaqII GACCGA 1 cut(s) 1002
TasI AATT 5 cut(s) 272, 295, 591, 749, 770
TatI WGTACW 3 cut(s) 278, 775, 824
TfiI GAWTC 4 cut(s) 154, 650, 833, 875
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 3 cut(s) 466, 718, 988
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 4 cut(s) 26, 236, 377, 667
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 3 cut(s) 183, 432, 867
TspRI CASTG 3 cut(s) 466, 718, 988
VpaK11BI GGWCC 1 cut(s) 455
VspI ATTAAT 1 cut(s) 294
XapI RAATTY 3 cut(s) 591, 749, 770
XbaI TCTAGA 2 cut(s) 815, 911
XmiI GTMKAC 2 cut(s) 481, 931
XspI CTAG 3 cut(s) 816, 912, 935
ZrmI AGTACT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.