Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr17

Orthogroup ID

OG0012086

Physical Location & Seq

Genomic Coordinates Reverse (-)

24932296 .. 24942160

Gene Structure 9865 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2010 bp

ATGGCCATGAAATTCCTTCTACTGCTTTTCTTGCTCAGCTTTCATCTTCGTCTCGCATTCTCTGGACAGACAAGCAAGATGGAGTCAGTTCCTGATCTTCAGAGTTCAATGTATATGACACTTGATGGATATCCTTGTGTACGGCTACTCAATCTTTCTGGGGAGATTGGTTGTTCAAATCCTGGAAGAGAGAAGGTTGTCGCTCCGATTATAAGATTGAACAATGCTACTGAGTTGTCTCAGTTATCTGCAGTTCTGTTGTCAGTTGATAAAATTCAAAGTTTTCTTGCCAGAATATTGAATGATTCAAATTTTGCTAAAAATGTTGCCGGTGTCTTGGTTGAATCCAGACCTGAGCTGCAAAATCAGTTGAAAGGATTCTCTCCAGCTCAAAAGTTCCCACAATCTGAATTTGCTCCTTACACTAGCATCAACTATGAATGGAACCCAACTGGCTCTGGTATAATGTGGAACCCATATAACTTTCCTGTATTCTTACTCTCGCAGACTAGCACATTGACCATCCAGGAGGCGGCCATAAAGAATCAGAAGAGCAAGAAATCTTATACTGATGATGTGGCTGAGTTTGATCTGGTAATGCAGACAATGAAATCTGGGACTCGTGATTCCGAATCTTGTCTAAAGGAAAACACTTGCCTTCCCTTAGGTGGATACAGTGTTTGGTCATCACTTCCCCCAATCAACATTTCATCACCAGAACACTCCAAGCCCATTATACTTGTACTGGCGTCAATGGATTCAGCTTCTTTTTTCCGTGATAAAGCACTCGGCGCCAACTCTCCTATATCTGGGCTGATTTCATTGCTGGCCACAGTTGATGCACTTTCTCGTGCGGATGATCTGGATGGCTTCAATAAACAGCTTGTTTTCGCCGTTTTCACTGGAGAGGCCTGGGGCTATCTTGGTAGCAGGAGATTTTTGCTTGAACTTGATCTGCAGTCTAATGCTGTTAGTGGTCTTAATTACTCATTGATTGAGAAGGTTGTGGAAATTGGATCTGTTGGAAAGGGCCTCAACCAAGGAGCTAAGAACTTTTTCATTCATACAGCTGGGGTTTCATCTTCCACAAATGAGACATTGGATGCTGTGAGTCGTGCCCAAGATTCAGTCAAGTCTGAAAACATTACAATCTCATCTGCAAATGCTTCAAATCCTGGGATACCTCCATCCTCCTTGATGACTTTTCTGAGAAAGAACTCTCTAACCTCTGGGGTTGTGTTGGAAGATTTTGATAATGTCTTCACCAACAAGTTCTACCACAGTCACCTTGATGATTTATCAAATATCAACTCATCATCCATTGCGGCAGCTGCTTCCCTTGTAGCACGCTCCCTTTACATTCTTGCAAGTGACACCAAAAATCTAAGCAGTTCTGCTCTGATGTCTATCAACGTGAATGCTTCACTAGTTGAAGAACTAGTGGGTTGCCTGGTAGACTGTGAGCCAGGTCTTTCTTGTGATCTGGTGAAAAGCTATATATCACCAGCCAATACCTGCCCGAGCCATTATGTTGGCGTTATACTTGATGAACCTTCCTCCACACCTGATCTGGGATACGTTGGTGACATTTCAAGGTTCGTTTGGAACTTCCTGGCCGAAAGAACTTCTATTCCGAAGGAAAATGCAAGCTCTGTTTGTACTCAAGACTGCAGTAATGATGGTGAAGTATGTATTAGAGCAGAAACAGAGGGAAAGGGTGTCTGTGTTGTCTCCACAACCAGATATGTTCCGGCATACTCAACACGGTTGAAATTTGAATCTGGTGGAACGTGGACTGTGTTGCCTCCGAATAACTCTGACCCCATGGGGCAGTTGGACGCTGTTTGGACAGAGAGCAACTGGGATGTGATCGGGCTTCGGGTCTACACTGTCCAAAATTCTTCTTTCGATCGTCTCGTCTTACTCGGGAGCATTGCCGTTACAGTCTTGGCTTACTTTGCAATAGTGATTTTCAAGGGCTGCGTGACGAAGGCTTTAAAACGCGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0010467 GO:0016020 GO:0016021 GO:0016485 GO:0016787 GO:0019538 GO:0031224 GO:0031226 GO:0043170 GO:0044238 GO:0044425 GO:0044459 GO:0044464 GO:0051604 GO:0070011 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04330 ko05010 map04330 map05010

Pfam Domains

Protein Families

Nicastrin

Protein Analysis

670

Amino Acids

72.56

Weight (kDa)

5.15

Isoelectric Point (pI)

40.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ncstrn_small	PF18266	45 - 202	4.6e-43	Nicastrin small lobe
Peptidase_M28	PF04389	242 - 450	1.2e-06	Peptidase family M28
Nicastrin	PF05450	244 - 453	1.6e-72	Nicastrin large lobe

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012086)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G52640 AT3G52640 AT3G52640
fragaria_vesca	FvH4_1g30060
malus_domestica	MD17G1267200.v1.1
prunus_persica	Prupe.3G165300_v2.0.a1 Prupe.3G165300_v2.0.a1
pyrus_communis	pycom17g26630
rosa_chinensis	RchiOBHm_Chr3g0492061
rosa_laevigata	RLG00000022797
rosa_multiflora	Rmu_sc0003846.1_g000020 Rmu_sc0042516.1_g000001
rosa_roxburghii	Rroxscaffold_6G00392600
rosa_rugosa	Rorug03G0251500
rosa_samantha	Rh3AG301700 Rh3BG337500 Rh3CG335000 Rh3DG337100
rosa_wichuraiana	Rw3G026630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	212
Acc36I	ACCTGC	1 cut(s)	1523
AccB1I	GGYRCC	1 cut(s)	791
AccI	GTMKAC	2 cut(s)	1455, 1884
AccII	CGCG	1 cut(s)	2004
AciI	CCGC	3 cut(s)	533, 854, 1325
AclWI	GGATC	1 cut(s)	1024
AcoI	YGGCCR	4 cut(s)	3, 534, 828, 1614
AcsI	RAATTY	6 cut(s)	11, 273, 310, 410, 1772, 1897
AcuI	CTGAAG	1 cut(s)	83
AcyI	GRCGYC	2 cut(s)	749, 792
AfaI	GTAC	3 cut(s)	141, 744, 1660
AfiI	CCNNNNNNNGG	4 cut(s)	532, 668, 809, 1571
AhlI	ACTAGT	2 cut(s)	1426, 1438
AjnI	CCWGG	7 cut(s)	181, 525, 911, 1174, 1449, 1465, 1611
AjuI	GAANNNNNNNTTGG	2 cut(s)	691, 723
AloI	GAACNNNNNNTCC	2 cut(s)	1615, 1647
Alw26I	GTCTC	5 cut(s)	56, 243, 1088, 1735, 1919
AlwI	GGATC	1 cut(s)	1024
AlwNI	CAGNNNCTG	1 cut(s)	92
Ama87I	CYCGRG	2 cut(s)	1519, 1925
AoxI	GGCC	6 cut(s)	3, 534, 828, 909, 1030, 1614
ApeKI	GCWGC	4 cut(s)	358, 1328, 1331, 1980
ApoI	RAATTY	6 cut(s)	11, 273, 310, 410, 1772, 1897
Asp700I	GAANNNNTTC	2 cut(s)	377, 1055
AspLEI	GCGC	1 cut(s)	794
AspS9I	GGNCC	1 cut(s)	1030
AsuHPI	GGTGA	7 cut(s)	705, 1255, 1277, 1494, 1498, 1595, 1694
AvaI	CYCGRG	2 cut(s)	1519, 1925
AxyI	CCTNAGG	1 cut(s)	664
BaeGI	GKGCMC	1 cut(s)	1120
BaeI	ACNNNNGTAYC	2 cut(s)	1567, 1600
BalI	TGGCCA	2 cut(s)	5, 830
BanI	GGYRCC	1 cut(s)	791
BauI	CACGAG	2 cut(s)	621, 849
BbsI	GAAGAC	1 cut(s)	1252
BbvI	GCAGC	4 cut(s)	345, 1318, 1340, 1967
BccI	CCATC	6 cut(s)	73, 119, 530, 860, 1195, 1673
BceAI	ACGGC	3 cut(s)	158, 878, 1922
BciT130I	CCWGG	7 cut(s)	183, 527, 913, 1176, 1451, 1467, 1613
BciVI	GTATCC	3 cut(s)	665, 1173, 1568
BcoDI	GTCTC	5 cut(s)	56, 243, 1088, 1735, 1919
BcuI	ACTAGT	2 cut(s)	1426, 1438
BfaI	CTAG	4 cut(s)	426, 510, 1427, 1439
BfmI	CTRYAG	3 cut(s)	249, 956, 1669
BfoI	RGCGCY	1 cut(s)	795
BfuAI	ACCTGC	1 cut(s)	1523
BfuI	GTATCC	3 cut(s)	665, 1173, 1568
BisI	GCNGC	6 cut(s)	359, 534, 1326, 1329, 1332, 1981
BlpI	GCTNAGC	1 cut(s)	35
BlsI	GCNGC	6 cut(s)	360, 535, 1327, 1330, 1333, 1982
Bme1390I	CCNGG	7 cut(s)	183, 527, 913, 1176, 1451, 1467, 1613
BmeT110I	CYCGRG	2 cut(s)	1519, 1925
BmgT120I	GGNCC	1 cut(s)	1030
BmiI	GGNNCC	3 cut(s)	446, 473, 793
BmrFI	CCNGG	7 cut(s)	183, 527, 913, 1176, 1451, 1467, 1613
BmrI	ACTGGG	1 cut(s)	1870
BmsI	GCATC	3 cut(s)	438, 829, 1093
BmuI	ACTGGG	1 cut(s)	1870
BpiI	GAAGAC	1 cut(s)	1252
BpmI	CTGGAG	2 cut(s)	369, 924
Bpu10I	CCTNAGC	1 cut(s)	354
Bpu1102I	GCTNAGC	1 cut(s)	35
BpuEI	CTTGAG	1 cut(s)	1647
BsaBI	GATNNNNATC	2 cut(s)	129, 1406
BsaHI	GRCGYC	2 cut(s)	749, 792
BsaJI	CCNNGG	4 cut(s)	912, 1039, 1175, 1824
Bsc4I	CCNNNNNNNGG	4 cut(s)	532, 668, 809, 1571
Bse118I	RCCGGY	1 cut(s)	329
Bse1I	ACTGG	4 cut(s)	457, 750, 907, 1865
Bse21I	CCTNAGG	1 cut(s)	664
Bse3DI	GCAATG	3 cut(s)	821, 1320, 1932
Bse8I	GATNNNNATC	2 cut(s)	129, 1406
BseBI	CCWGG	7 cut(s)	183, 527, 913, 1176, 1451, 1467, 1613
BseDI	CCNNGG	4 cut(s)	912, 1039, 1175, 1824
BseGI	GGATG	7 cut(s)	522, 862, 871, 1108, 1187, 1316, 1870
BseJI	GATNNNNATC	2 cut(s)	129, 1406
BseLI	CCNNNNNNNGG	4 cut(s)	532, 668, 809, 1571
BseMI	GCAATG	3 cut(s)	821, 1320, 1932
BseMII	CTCAG	6 cut(s)	49, 222, 254, 345, 573, 1199
BseNI	ACTGG	4 cut(s)	457, 750, 907, 1865
BseSI	GKGCMC	1 cut(s)	1120
BseXI	GCAGC	4 cut(s)	345, 1318, 1340, 1967
BseYI	CCCAGC	1 cut(s)	1070
Bsh1236I	CGCG	1 cut(s)	2004
Bsh1285I	CGRYCG	1 cut(s)	1912
BshFI	GGCC	6 cut(s)	5, 536, 830, 911, 1032, 1616
BshNI	GGYRCC	1 cut(s)	791
BsiEI	CGRYCG	1 cut(s)	1912
BsiHKCI	CYCGRG	2 cut(s)	1519, 1925
BsiSI	CCGG	2 cut(s)	330, 1751
BslFI	GGGAC	1 cut(s)	631
BslI	CCNNNNNNNGG	4 cut(s)	532, 668, 809, 1571
BsmAI	GTCTC	5 cut(s)	56, 243, 1088, 1735, 1919
BsmBI	CGTCTC	2 cut(s)	56, 1919
BsmFI	GGGAC	1 cut(s)	631
BsmI	GAATGC	2 cut(s)	56, 1423
BsnI	GGCC	6 cut(s)	5, 536, 830, 911, 1032, 1616
BsoBI	CYCGRG	2 cut(s)	1519, 1925
Bsp1286I	GDGCHC	1 cut(s)	1120
Bsp143I	GATC	9 cut(s)	94, 589, 859, 952, 1016, 1480, 1567, 1869, 1909
Bsp1720I	GCTNAGC	1 cut(s)	35
Bsp19I	CCATGG	1 cut(s)	1824
BspACI	CCGC	3 cut(s)	533, 854, 1325
BspANI	GGCC	6 cut(s)	5, 536, 830, 911, 1032, 1616
BspCNI	CTCAG	6 cut(s)	48, 223, 253, 346, 574, 1200
BspFNI	CGCG	1 cut(s)	2004
BspLI	GGNNCC	3 cut(s)	446, 473, 793
BspMAI	CTGCAG	3 cut(s)	253, 960, 1673
BspMI	ACCTGC	1 cut(s)	1523
BspPI	GGATC	1 cut(s)	1024
BspQI	GCTCTTC	1 cut(s)	545
BspT107I	GGYRCC	1 cut(s)	791
BsrDI	GCAATG	3 cut(s)	821, 1320, 1932
BsrFI	RCCGGY	1 cut(s)	329
BsrI	ACTGG	4 cut(s)	457, 750, 907, 1865
BssAI	RCCGGY	1 cut(s)	329
BssECI	CCNNGG	4 cut(s)	912, 1039, 1175, 1824
BssMI	GATC	9 cut(s)	94, 589, 859, 952, 1016, 1480, 1567, 1869, 1909
BssNI	GRCGYC	2 cut(s)	749, 792
BssSI	CACGAG	2 cut(s)	621, 849
BssT1I	CCWWGG	2 cut(s)	1039, 1824
Bst2BI	CACGAG	2 cut(s)	621, 849
Bst2UI	CCWGG	7 cut(s)	183, 527, 913, 1176, 1451, 1467, 1613
Bst4CI	ACNGT	8 cut(s)	677, 835, 1283, 1460, 1767, 1798, 1891, 1945
Bst6I	CTCTTC	2 cut(s)	181, 545
BstACI	GRCGYC	2 cut(s)	749, 792
BstC8I	GCNNGC	3 cut(s)	828, 1348, 1648
BstDEI	CTNAG	9 cut(s)	35, 231, 240, 354, 582, 664, 1047, 1208, 1385
BstDSI	CCRYGG	1 cut(s)	1824
BstF5I	GGATG	7 cut(s)	522, 862, 871, 1108, 1187, 1316, 1870
BstFNI	CGCG	1 cut(s)	2004
BstH2I	RGCGCY	1 cut(s)	795
BstHHI	GCGC	1 cut(s)	794
BstKTI	GATC	9 cut(s)	97, 592, 862, 955, 1019, 1483, 1570, 1872, 1912
BstMAI	GTCTC	5 cut(s)	56, 243, 1088, 1735, 1919
BstMBI	GATC	9 cut(s)	94, 589, 859, 952, 1016, 1480, 1567, 1869, 1909
BstMCI	CGRYCG	1 cut(s)	1912
BstMWI	GCNNNNNNNGC	4 cut(s)	31, 791, 1331, 1958
BstNI	CCWGG	7 cut(s)	183, 527, 913, 1176, 1451, 1467, 1613
BstSCI	CCNGG	7 cut(s)	181, 525, 911, 1174, 1449, 1465, 1611
BstSFI	CTRYAG	3 cut(s)	249, 956, 1669
BstSLI	GKGCMC	1 cut(s)	1120
BstUI	CGCG	1 cut(s)	2004
BstV1I	GCAGC	4 cut(s)	345, 1318, 1340, 1967
BstV2I	GAAGAC	1 cut(s)	1252
BstX2I	RGATCY	1 cut(s)	1016
BstXI	CCANNNNNNTGG	1 cut(s)	1532
BstYI	RGATCY	1 cut(s)	1016
Bsu36I	CCTNAGG	1 cut(s)	664
BsuI	GTATCC	3 cut(s)	665, 1173, 1568
BsuRI	GGCC	6 cut(s)	5, 536, 830, 911, 1032, 1616
BtgI	CCRYGG	1 cut(s)	1824
BtsCI	GGATG	7 cut(s)	522, 862, 871, 1108, 1187, 1316, 1870
BtsIMutI	CAGTG	3 cut(s)	682, 900, 1887
BveI	ACCTGC	1 cut(s)	1523
Cac8I	GCNNGC	3 cut(s)	828, 1348, 1648
CaiI	CAGNNNCTG	1 cut(s)	92
CfoI	GCGC	1 cut(s)	794
Cfr10I	RCCGGY	1 cut(s)	329
Cfr13I	GGNCC	1 cut(s)	1030
CseI	GACGC	2 cut(s)	738, 1847
Csp6I	GTAC	3 cut(s)	140, 743, 1659
CviAII	CATG	2 cut(s)	7, 1825
CviQI	GTAC	3 cut(s)	140, 743, 1659
DdeI	CTNAG	9 cut(s)	35, 231, 240, 354, 582, 664, 1047, 1208, 1385
DinI	GGCGCC	1 cut(s)	793
DpnI	GATC	9 cut(s)	96, 591, 861, 954, 1018, 1482, 1569, 1871, 1911
DpnII	GATC	9 cut(s)	94, 589, 859, 952, 1016, 1480, 1567, 1869, 1909
DraI	TTTAAA	1 cut(s)	1998
EaeI	YGGCCR	4 cut(s)	3, 534, 828, 1614
Eam1104I	CTCTTC	2 cut(s)	181, 545
EarI	CTCTTC	2 cut(s)	181, 545
Eco130I	CCWWGG	2 cut(s)	1039, 1824
Eco147I	AGGCCT	1 cut(s)	911
Eco32I	GATATC	1 cut(s)	131
Eco57I	CTGAAG	1 cut(s)	83
Eco81I	CCTNAGG	1 cut(s)	664
Eco88I	CYCGRG	2 cut(s)	1519, 1925
EcoO109I	RGGNCCY	1 cut(s)	1030
EcoRII	CCWGG	7 cut(s)	181, 525, 911, 1174, 1449, 1465, 1611
EcoRV	GATATC	1 cut(s)	131
EcoT14I	CCWWGG	2 cut(s)	1039, 1824
EgeI	GGCGCC	1 cut(s)	793
EheI	GGCGCC	1 cut(s)	793
ErhI	CCWWGG	2 cut(s)	1039, 1824
Esp3I	CGTCTC	2 cut(s)	56, 1919
FaeI	CATG	2 cut(s)	10, 1828
FaqI	GGGAC	1 cut(s)	631
FatI	CATG	2 cut(s)	6, 1824
FblI	GTMKAC	2 cut(s)	1455, 1884
Fnu4HI	GCNGC	6 cut(s)	359, 534, 1326, 1329, 1332, 1981
FokI	GGATG	7 cut(s)	509, 869, 878, 1115, 1174, 1303, 1877
Fsp4HI	GCNGC	6 cut(s)	359, 534, 1326, 1329, 1332, 1981
FspBI	CTAG	4 cut(s)	426, 510, 1427, 1439
GlaI	GCGC	1 cut(s)	793
GluI	GCNGC	6 cut(s)	359, 534, 1326, 1329, 1332, 1981
GsaI	CCCAGC	1 cut(s)	1074
GsuI	CTGGAG	2 cut(s)	369, 924
HaeII	RGCGCY	1 cut(s)	795
HaeIII	GGCC	6 cut(s)	5, 536, 830, 911, 1032, 1616
HapII	CCGG	2 cut(s)	330, 1751
HgaI	GACGC	2 cut(s)	738, 1847
HhaI	GCGC	1 cut(s)	794
Hin1I	GRCGYC	2 cut(s)	749, 792
Hin1II	CATG	2 cut(s)	10, 1828
Hin6I	GCGC	1 cut(s)	792
HinP1I	GCGC	1 cut(s)	792
HpaII	CCGG	2 cut(s)	330, 1751
HphI	GGTGA	7 cut(s)	705, 1255, 1277, 1494, 1498, 1595, 1694
Hpy166II	GTNNAC	4 cut(s)	140, 1456, 1794, 1885
Hpy188III	TCNNGA	7 cut(s)	63, 92, 348, 623, 863, 1664, 1927
Hpy8I	GTNNAC	4 cut(s)	140, 1456, 1794, 1885
HpyAV	CCTTC	7 cut(s)	26, 187, 668, 994, 1563, 1630, 1984
HpyCH4III	ACNGT	8 cut(s)	677, 835, 1283, 1460, 1767, 1798, 1891, 1945
HpyCH4IV	ACGT	3 cut(s)	1413, 1578, 1790
HpyF10VI	GCNNNNNNNGC	4 cut(s)	31, 791, 1331, 1958
HpyF3I	CTNAG	9 cut(s)	35, 231, 240, 354, 582, 664, 1047, 1208, 1385
HpySE526I	ACGT	3 cut(s)	1413, 1578, 1790
Hsp92I	GRCGYC	2 cut(s)	749, 792
Hsp92II	CATG	2 cut(s)	10, 1828
HspAI	GCGC	1 cut(s)	792
KasI	GGCGCC	1 cut(s)	791
Kzo9I	GATC	9 cut(s)	94, 589, 859, 952, 1016, 1480, 1567, 1869, 1909
LguI	GCTCTTC	1 cut(s)	545
LmnI	GCTCC	5 cut(s)	208, 421, 1043, 1355, 1929
Lsp1109I	GCAGC	4 cut(s)	345, 1318, 1340, 1967
LweI	GCATC	3 cut(s)	438, 829, 1093
MaeI	CTAG	4 cut(s)	426, 510, 1427, 1439
MaeII	ACGT	3 cut(s)	1413, 1578, 1790
MaeIII	GTNAC	5 cut(s)	1283, 1370, 1583, 1939, 1984
MalI	GATC	9 cut(s)	96, 591, 861, 954, 1018, 1482, 1569, 1871, 1911
MboI	GATC	9 cut(s)	94, 589, 859, 952, 1016, 1480, 1567, 1869, 1909
MboII	GAAGA	9 cut(s)	38, 89, 198, 562, 1074, 1252, 1256, 1445, 1893
MflI	RGATCY	1 cut(s)	1016
MhlI	GDGCHC	1 cut(s)	1120
MlsI	TGGCCA	2 cut(s)	5, 830
MluCI	AATT	8 cut(s)	11, 273, 310, 410, 982, 1011, 1772, 1897
MluNI	TGGCCA	2 cut(s)	5, 830
Mly113I	GGCGCC	1 cut(s)	792
MlyI	GAGTC	3 cut(s)	92, 613, 1120
MmeI	TCCRAC	3 cut(s)	1003, 1221, 1815
MnlI	CCTC	9 cut(s)	523, 901, 1043, 1194, 1201, 1237, 1567, 1702, 1815
Mox20I	TGGCCA	2 cut(s)	5, 830
MroXI	GAANNNNTTC	2 cut(s)	377, 1055
MscI	TGGCCA	2 cut(s)	5, 830
MseI	TTAA	2 cut(s)	981, 1997
MslI	CAYNNNNRTG	1 cut(s)	1290
Msp20I	TGGCCA	2 cut(s)	5, 830
MspA1I	CMGCKG	2 cut(s)	1070, 1331
MspI	CCGG	2 cut(s)	330, 1751
MspR9I	CCNGG	7 cut(s)	183, 527, 913, 1176, 1451, 1467, 1613
Mva1269I	GAATGC	2 cut(s)	56, 1423
MvaI	CCWGG	7 cut(s)	183, 527, 913, 1176, 1451, 1467, 1613
MvnI	CGCG	1 cut(s)	2004
MwoI	GCNNNNNNNGC	4 cut(s)	31, 791, 1331, 1958
NarI	GGCGCC	1 cut(s)	792
NcoI	CCATGG	1 cut(s)	1824
NdeII	GATC	9 cut(s)	94, 589, 859, 952, 1016, 1480, 1567, 1869, 1909
NlaIII	CATG	2 cut(s)	10, 1828
NlaIV	GGNNCC	3 cut(s)	446, 473, 793
NmeAIII	GCCGAG	1 cut(s)	768
NmuCI	GTSAC	4 cut(s)	1283, 1370, 1583, 1984
PceI	AGGCCT	1 cut(s)	911
PciSI	GCTCTTC	1 cut(s)	545
PcsI	WCGNNNNNNNCGW	1 cut(s)	1914
PctI	GAATGC	2 cut(s)	56, 1423
PdmI	GAANNNNTTC	2 cut(s)	377, 1055
PfeI	GAWTC	9 cut(s)	305, 344, 378, 544, 626, 632, 758, 1124, 1778
PfoI	TCCNGGA	2 cut(s)	181, 525
PkrI	GCNGC	6 cut(s)	360, 535, 1327, 1330, 1333, 1982
Ple19I	CGATCG	1 cut(s)	1912
PleI	GAGTC	3 cut(s)	91, 613, 1119
PluTI	GGCGCC	1 cut(s)	795
PpsI	GAGTC	3 cut(s)	91, 613, 1119
PsiI	TTATAA	1 cut(s)	212
Psp6I	CCWGG	7 cut(s)	181, 525, 911, 1174, 1449, 1465, 1611
PspFI	CCCAGC	1 cut(s)	1070
PspGI	CCWGG	7 cut(s)	181, 525, 911, 1174, 1449, 1465, 1611
PspN4I	GGNNCC	3 cut(s)	446, 473, 793
PspPI	GGNCC	1 cut(s)	1030
PstI	CTGCAG	3 cut(s)	253, 960, 1673
PstNI	CAGNNNCTG	1 cut(s)	92
PsuI	RGATCY	1 cut(s)	1016
PvuI	CGATCG	1 cut(s)	1912
PvuII	CAGCTG	2 cut(s)	1070, 1331
RsaI	GTAC	3 cut(s)	141, 744, 1660
RsaNI	GTAC	3 cut(s)	140, 743, 1659
RseI	CAYNNNNRTG	1 cut(s)	1290
SapI	GCTCTTC	1 cut(s)	545
SaqAI	TTAA	2 cut(s)	981, 1997
SatI	GCNGC	6 cut(s)	359, 534, 1326, 1329, 1332, 1981
Sau3AI	GATC	9 cut(s)	94, 589, 859, 952, 1016, 1480, 1567, 1869, 1909
Sau96I	GGNCC	1 cut(s)	1030
SchI	GAGTC	3 cut(s)	92, 613, 1120
ScrFI	CCNGG	7 cut(s)	183, 527, 913, 1176, 1451, 1467, 1613
SduI	GDGCHC	1 cut(s)	1120
SfaNI	GCATC	3 cut(s)	438, 829, 1093
SfcI	CTRYAG	3 cut(s)	249, 956, 1669
SfoI	GGCGCC	1 cut(s)	793
SmiMI	CAYNNNNRTG	1 cut(s)	1290
SmlI	CTYRAG	1 cut(s)	1662
SmoI	CTYRAG	1 cut(s)	1662
SpeI	ACTAGT	2 cut(s)	1426, 1438
Sse9I	AATT	8 cut(s)	11, 273, 310, 410, 982, 1011, 1772, 1897
SseBI	AGGCCT	1 cut(s)	911
SsiI	CCGC	3 cut(s)	533, 854, 1325
SspDI	GGCGCC	1 cut(s)	791
SspI	AATATT	1 cut(s)	297
SspMI	CTAG	4 cut(s)	426, 510, 1427, 1439
StuI	AGGCCT	1 cut(s)	911
StyD4I	CCNGG	7 cut(s)	181, 525, 911, 1174, 1449, 1465, 1611
StyI	CCWWGG	2 cut(s)	1039, 1824
TaaI	ACNGT	8 cut(s)	677, 835, 1283, 1460, 1767, 1798, 1891, 1945
TaiI	ACGT	3 cut(s)	1416, 1581, 1793
TaqI	TCGA	1 cut(s)	1908
TasI	AATT	8 cut(s)	11, 273, 310, 410, 982, 1011, 1772, 1897
TatI	WGTACW	2 cut(s)	742, 1658
TauI	GCSGC	2 cut(s)	536, 1328
TfiI	GAWTC	9 cut(s)	305, 344, 378, 544, 626, 632, 758, 1124, 1778
Tru1I	TTAA	2 cut(s)	981, 1997
Tru9I	TTAA	2 cut(s)	981, 1997
TscAI	CASTG	3 cut(s)	682, 907, 1894
TseFI	GTSAC	4 cut(s)	1283, 1370, 1583, 1984
TseI	GCWGC	4 cut(s)	358, 1328, 1331, 1980
Tsp45I	GTSAC	4 cut(s)	1283, 1370, 1583, 1984
TspGWI	ACGGA	1 cut(s)	764
TspRI	CASTG	3 cut(s)	682, 907, 1894
XapI	RAATTY	6 cut(s)	11, 273, 310, 410, 1772, 1897
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1567, 1831
XmiI	GTMKAC	2 cut(s)	1455, 1884
XmnI	GAANNNNTTC	2 cut(s)	377, 1055
XspI	CTAG	4 cut(s)	426, 510, 1427, 1439

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.