pycom17g27050

Polyamine oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0011767
Physical Location & Seq
Forward (+)
25300659 .. 25305118
4460 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1482 bp
ATGGACCCGAAGGATCCATTCTCTGCTGTATTATTTGATGGCTCTGTTTCGTCTCACATTGGGAGGCAACAGAATGCATGCCCTTCTGTTATTGTTATTGGTGGTGGGATATCAGGGATTGCTGCTGCACGTATTCTCCATGATGCATCTTTTAAGGTGATTTTGCTCGAATCACGGGACAGACTTGGTGGCCGGATTCATACGGACTACTCATTTGGTTGTCCAGTCGATATGGGAGCATCATGGCTACATGGTGTTTGCAACGAGAATCCTTTGGCTCCACTGATACGCCGCTTAGGACTTACTCTATACCGTACGAGTGGCGATGACTCTGTGTTGTATGACCACGATTTGGAAAGCTTTGCACTTTTTGATACGGATGGCTGCCAAGTTCCTCAAAAGATAGTCGTTGAAGTTGGTGATACTTTCACGAAAATTCTCAAAGAGACTGAGAAAGTAAGGAATGAGCATACCGATGACATGTCCGTTTCTCAAGCAATTTCTGTTGTGATGGATAGGCATCCTGAATTAAGACAAAACGGACTTGCTCACGAAGTGTTACAATGGTACATATGTCGAATGGAAGCGTGGTTTGCTGCAGATGCAGATGTAATATCACTAAAAAACTGGGATCAGGAGCATGTTCTATCCGGTGGTCATGGACTTATGGTGCAAGGGTATGACCCAATAATAAAGGCTCTTGCAAAAGATATTGATGTACGCTTGAATCACAGGGTGATAAAGATATTAAATGGGTCTAACAAGGTGATGGTCACAATTGAGGACGGAAGAAACTTCATTGCAGATGCTGCTATAATAACAGTACCCCATGGGATTCTTAAAGCCAAGTTGATTGAGTTCGTACCCCAGTTGCCTGAGTGGAAGGTTGCTGCAATTTCTGATCTCGGTGTGGGCAATGAAAACAAAGTTGCCTTACGGTTTGAGAAAGTTTTCTGGCCAAATGTAGAGCTCTTGGGCATTGTTGCACCAAATTCTTTTGCGTGTGGTTATTTTCTCAATCTTCACAAGGCCACAGGCCATCCGGTCCTCGTTTACATGGCTGCTGGAAGGTTTGCATATGACCTTGAAAAACTAACGAACGATGCTGCCGTTAATTTTGTGATGTTGCAGCTTAAGAAGATGTTGCCTGATGCCACTGAACCAGTTCAATATCTGGTATCACGATGGGGAACGGACCTGAATTCTCTTGGATGTTACTCGTTGGATTTGGTTGGAAAGCCAGGAGATATATATGAGAGGCTTCGGGCACCTTTGGGTAATCTCTTTTTCGGTGGGGAAGCTGTTAGCATGGACCACCAAGGATCTGTACACGGAGCTTACTCGGCAGGAGTTATGGCTGCTGAGAACTGTCAGCAGCATCTGTTGCAGAAACTCGGTAATTTGGAGACCCTTCAGCATGCTTACCTTATGGAAGAAGTGCTTGAAGCAGCAACAGTTCCCCTCCAAATCTCAAGGATGTGA

Protein Analysis

494

Amino Acids

54.31

Weight (kDa)

5.55

Isoelectric Point (pI)

32.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_8 PF13450 32 - 98 8.6e-14 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 37 - 456 1.3e-84 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011767)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65840
fragaria_vesca FvH4_1g27430
malus_domestica MD09G1280300.v1.1 MD17G1287500.v1.1
prunus_persica Prupe.3G013800_v2.0.a1 Prupe.3G013800_v2.0.a1
pyrus_communis pycom09g18840 pycom17g27050
rosa_chinensis RchiOBHm_Chr3g0488611
rosa_laevigata RLG00000022940
rosa_multiflora Rmu_sc0005363.1_g000049
rosa_roxburghii Rroxscaffold_6G00394100
rosa_rugosa Rorug03G0239500 Rorug03G0239600
rosa_samantha Rh3AG288500 Rh3BG325200 Rh3CG322800 Rh3DG321500
rosa_wichuraiana Rw3G025530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1267
AccB7I CCANNNNNTGG 1 cut(s) 352
AciI CCGC 1 cut(s) 292
AclWI GGATC 4 cut(s) 8, 21, 639, 1330
AcoI YGGCCR 2 cut(s) 190, 956
AcsI RAATTY 3 cut(s) 435, 991, 1201
AcuI CTGAAG 1 cut(s) 1397
AdeI CACNNNGTG 2 cut(s) 556, 736
AfaI GTAC 6 cut(s) 316, 569, 720, 825, 864, 1329
AfiI CCNNNNNNNGG 1 cut(s) 352
AflII CTTAAG 1 cut(s) 1133
AflIII ACRYGT 1 cut(s) 480
AgsI TTSAA 5 cut(s) 413, 727, 1088, 1169, 1445
AjnI CCWGG 1 cut(s) 1240
AluBI AGCT 5 cut(s) 360, 970, 1132, 1301, 1337
AluI AGCT 5 cut(s) 360, 970, 1132, 1301, 1337
Alw21I GWGCWC 1 cut(s) 972
Alw26I GTCTC 3 cut(s) 57, 440, 1400
AlwI GGATC 4 cut(s) 8, 21, 639, 1330
AlwNI CAGNNNCTG 2 cut(s) 809, 1381
AoxI GGCC 4 cut(s) 190, 956, 1029, 1036
ApoI RAATTY 3 cut(s) 435, 991, 1201
ArsI GACNNNNNNTTYG 2 cut(s) 197, 229
Asp700I GAANNNNTTC 2 cut(s) 950, 1164
AspS9I GGNCC 4 cut(s) 4, 1045, 1195, 1312
AsuHPI GGTGA 4 cut(s) 169, 431, 748, 778
AvaII GGWCC 4 cut(s) 4, 1045, 1195, 1312
BaeGI GKGCMC 1 cut(s) 1270
BaeI ACNNNNGTAYC 2 cut(s) 559, 592
BalI TGGCCA 1 cut(s) 958
BamHI GGATCC 1 cut(s) 13
BanI GGYRCC 1 cut(s) 1267
BanII GRGCYC 1 cut(s) 972
Bbv12I GWGCWC 1 cut(s) 972
BccI CCATC 6 cut(s) 32, 374, 505, 763, 1047, 1179
BceAI ACGGC 1 cut(s) 1094
BciT130I CCWGG 1 cut(s) 1242
BcoDI GTCTC 3 cut(s) 57, 440, 1400
BfmI CTRYAG 1 cut(s) 597
BfrI CTTAAG 1 cut(s) 1133
Bme1390I CCNGG 1 cut(s) 1242
Bme18I GGWCC 4 cut(s) 4, 1045, 1195, 1312
BmgT120I GGNCC 4 cut(s) 4, 1045, 1195, 1312
BmiI GGNNCC 4 cut(s) 6, 15, 279, 1269
BmrFI CCNGG 1 cut(s) 1242
BmrI ACTGGG 2 cut(s) 637, 862
BmsI GCATC 9 cut(s) 133, 155, 248, 529, 592, 796, 1093, 1141, 1387
BmuI ACTGGG 2 cut(s) 637, 862
Bpu10I CCTNAGC 1 cut(s) 295
BpuEI CTTGAG 2 cut(s) 477, 1456
BsaAI YACGTR 1 cut(s) 131
BsaBI GATNNNNATC 1 cut(s) 519
BsaI GGTCTC 1 cut(s) 1400
BsaJI CCNNGG 2 cut(s) 829, 1318
BsaWI WCCGGW 2 cut(s) 650, 1042
BsaXI ACNNNNNCTCC 2 cut(s) 120, 150
Bsc4I CCNNNNNNNGG 1 cut(s) 352
Bse1I ACTGG 4 cut(s) 224, 632, 868, 1163
Bse3DI GCAATG 2 cut(s) 798, 922
Bse8I GATNNNNATC 1 cut(s) 519
BseBI CCWGG 1 cut(s) 1242
BseDI CCNNGG 2 cut(s) 829, 1318
BseGI GGATG 5 cut(s) 385, 520, 1039, 1217, 1482
BseJI GATNNNNATC 1 cut(s) 519
BseLI CCNNNNNNNGG 1 cut(s) 352
BseMI GCAATG 2 cut(s) 798, 922
BseMII CTCAG 3 cut(s) 441, 867, 1353
BseNI ACTGG 4 cut(s) 224, 632, 868, 1163
BseSI GKGCMC 1 cut(s) 1270
BsgI GTGCAG 1 cut(s) 111
BshFI GGCC 4 cut(s) 192, 958, 1031, 1038
BshNI GGYRCC 1 cut(s) 1267
BsiHKAI GWGCWC 1 cut(s) 972
BsiSI CCGG 3 cut(s) 193, 651, 1043
BsiWI CGTACG 1 cut(s) 314
BslFI GGGAC 1 cut(s) 191
BslI CCNNNNNNNGG 1 cut(s) 352
BsmAI GTCTC 3 cut(s) 57, 440, 1400
BsmBI CGTCTC 1 cut(s) 57
BsmFI GGGAC 1 cut(s) 191
BsmI GAATGC 1 cut(s) 79
BsnI GGCC 4 cut(s) 192, 958, 1031, 1038
Bso31I GGTCTC 1 cut(s) 1400
Bsp1286I GDGCHC 2 cut(s) 972, 1270
Bsp1407I TGTACA 1 cut(s) 1327
Bsp143I GATC 4 cut(s) 13, 631, 901, 1322
Bsp19I CCATGG 1 cut(s) 829
BspACI CCGC 1 cut(s) 292
BspANI GGCC 4 cut(s) 192, 958, 1031, 1038
BspCNI CTCAG 3 cut(s) 442, 868, 1354
BspLI GGNNCC 4 cut(s) 6, 15, 279, 1269
BspMAI CTGCAG 1 cut(s) 601
BspPI GGATC 4 cut(s) 8, 21, 639, 1330
BspT107I GGYRCC 1 cut(s) 1267
BspTI CTTAAG 1 cut(s) 1133
BspTNI GGTCTC 1 cut(s) 1400
BsrDI GCAATG 2 cut(s) 798, 922
BsrGI TGTACA 1 cut(s) 1327
BsrI ACTGG 4 cut(s) 224, 632, 868, 1163
BssECI CCNNGG 2 cut(s) 829, 1318
BssMI GATC 4 cut(s) 13, 631, 901, 1322
BssT1I CCWWGG 2 cut(s) 829, 1318
Bst2UI CCWGG 1 cut(s) 1242
Bst4CI ACNGT 5 cut(s) 314, 823, 939, 1370, 1456
BstAFI CTTAAG 1 cut(s) 1133
BstAPI GCANNNNNTGC 2 cut(s) 809, 1384
BstAUI TGTACA 1 cut(s) 1327
BstBAI YACGTR 1 cut(s) 131
BstC8I GCNNGC 2 cut(s) 79, 1419
BstDEI CTNAG 4 cut(s) 295, 450, 876, 1362
BstDSI CCRYGG 1 cut(s) 829
BstF5I GGATG 5 cut(s) 385, 520, 1039, 1217, 1482
BstKTI GATC 4 cut(s) 16, 634, 904, 1325
BstMAI GTCTC 3 cut(s) 57, 440, 1400
BstMBI GATC 4 cut(s) 13, 631, 901, 1322
BstMWI GCNNNNNNNGC 5 cut(s) 593, 602, 809, 1343, 1384
BstNI CCWGG 1 cut(s) 1242
BstNSI RCATGY 4 cut(s) 81, 484, 644, 1421
BstSCI CCNGG 1 cut(s) 1240
BstSFI CTRYAG 1 cut(s) 597
BstSLI GKGCMC 1 cut(s) 1270
BstX2I RGATCY 2 cut(s) 13, 1322
BstYI RGATCY 2 cut(s) 13, 1322
BsuRI GGCC 4 cut(s) 192, 958, 1031, 1038
BtgI CCRYGG 1 cut(s) 829
BtgZI GCGATG 1 cut(s) 339
BtsCI GGATG 5 cut(s) 385, 520, 1039, 1217, 1482
BtsIMutI CAGTG 2 cut(s) 281, 1155
Cac8I GCNNGC 2 cut(s) 79, 1419
CaiI CAGNNNCTG 2 cut(s) 809, 1381
Cfr13I GGNCC 4 cut(s) 4, 1045, 1195, 1312
Csp6I GTAC 6 cut(s) 315, 568, 719, 824, 863, 1328
CviQI GTAC 6 cut(s) 315, 568, 719, 824, 863, 1328
DdeI CTNAG 4 cut(s) 295, 450, 876, 1362
DpnI GATC 4 cut(s) 15, 633, 903, 1324
DpnII GATC 4 cut(s) 13, 631, 901, 1322
DraIII CACNNNGTG 2 cut(s) 556, 736
EaeI YGGCCR 2 cut(s) 190, 956
Ecl136II GAGCTC 1 cut(s) 970
Eco130I CCWWGG 2 cut(s) 829, 1318
Eco24I GRGCYC 1 cut(s) 972
Eco31I GGTCTC 1 cut(s) 1400
Eco32I GATATC 1 cut(s) 111
Eco47I GGWCC 4 cut(s) 4, 1045, 1195, 1312
Eco53kI GAGCTC 1 cut(s) 970
Eco57I CTGAAG 1 cut(s) 1397
EcoICRI GAGCTC 1 cut(s) 970
EcoRI GAATTC 1 cut(s) 1201
EcoRII CCWGG 1 cut(s) 1240
EcoRV GATATC 1 cut(s) 111
EcoT14I CCWWGG 2 cut(s) 829, 1318
EcoT22I ATGCAT 2 cut(s) 79, 148
EcoT38I GRGCYC 1 cut(s) 972
ErhI CCWWGG 2 cut(s) 829, 1318
Esp3I CGTCTC 1 cut(s) 57
FalI AAGNNNNNCTT 2 cut(s) 1425, 1457
FaqI GGGAC 1 cut(s) 191
FauNDI CATATG 2 cut(s) 572, 1078
FokI GGATG 4 cut(s) 392, 507, 1026, 1224
FriOI GRGCYC 1 cut(s) 972
HaeIII GGCC 4 cut(s) 192, 958, 1031, 1038
HapII CCGG 3 cut(s) 193, 651, 1043
HindIII AAGCTT 1 cut(s) 358
HinfI GANTC 6 cut(s) 170, 196, 268, 329, 727, 835
HpaII CCGG 3 cut(s) 193, 651, 1043
HphI GGTGA 4 cut(s) 169, 431, 748, 778
Hpy166II GTNNAC 2 cut(s) 1054, 1330
Hpy188I TCNGA 1 cut(s) 901
Hpy188III TCNNGA 5 cut(s) 430, 524, 551, 635, 1182
Hpy8I GTNNAC 2 cut(s) 1054, 1330
HpyAV CCTTC 5 cut(s) 4, 93, 877, 1062, 1421
HpyCH4III ACNGT 5 cut(s) 314, 823, 939, 1370, 1456
HpyCH4IV ACGT 1 cut(s) 130
HpyF10VI GCNNNNNNNGC 5 cut(s) 593, 602, 809, 1343, 1384
HpyF3I CTNAG 4 cut(s) 295, 450, 876, 1362
HpySE526I ACGT 1 cut(s) 130
Kzo9I GATC 4 cut(s) 13, 631, 901, 1322
LmnI GCTCC 4 cut(s) 236, 283, 637, 1334
LweI GCATC 9 cut(s) 133, 155, 248, 529, 592, 796, 1093, 1141, 1387
MaeII ACGT 1 cut(s) 130
MaeIII GTNAC 3 cut(s) 558, 772, 1214
MalI GATC 4 cut(s) 15, 633, 903, 1324
MboI GATC 4 cut(s) 13, 631, 901, 1322
MboII GAAGA 4 cut(s) 801, 1013, 1150, 1445
MfeI CAATTG 1 cut(s) 777
MflI RGATCY 2 cut(s) 13, 1322
MhlI GDGCHC 2 cut(s) 972, 1270
MlsI TGGCCA 1 cut(s) 958
MluCI AATT 9 cut(s) 435, 498, 527, 777, 894, 991, 1114, 1201, 1399
MluNI TGGCCA 1 cut(s) 958
MlyI GAGTC 1 cut(s) 323
MmeI TCCRAC 2 cut(s) 1203, 1213
MnlI CCTC 6 cut(s) 57, 405, 775, 1058, 1251, 1472
Mox20I TGGCCA 1 cut(s) 958
Mph1103I ATGCAT 2 cut(s) 79, 148
MroXI GAANNNNTTC 2 cut(s) 950, 1164
MscI TGGCCA 1 cut(s) 958
MseI TTAA 6 cut(s) 153, 530, 749, 840, 1113, 1134
MslI CAYNNNNRTG 1 cut(s) 474
Msp20I TGGCCA 1 cut(s) 958
MspCI CTTAAG 1 cut(s) 1133
MspI CCGG 3 cut(s) 193, 651, 1043
MspR9I CCNGG 1 cut(s) 1242
MunI CAATTG 1 cut(s) 777
Mva1269I GAATGC 1 cut(s) 79
MvaI CCWGG 1 cut(s) 1242
MwoI GCNNNNNNNGC 5 cut(s) 593, 602, 809, 1343, 1384
NcoI CCATGG 1 cut(s) 829
NdeI CATATG 2 cut(s) 572, 1078
NdeII GATC 4 cut(s) 13, 631, 901, 1322
NlaIV GGNNCC 4 cut(s) 6, 15, 279, 1269
NmeAIII GCCGAG 1 cut(s) 1322
NmuCI GTSAC 1 cut(s) 772
NsiI ATGCAT 2 cut(s) 79, 148
NspI RCATGY 4 cut(s) 81, 484, 644, 1421
PaeI GCATGC 2 cut(s) 81, 1421
PciI ACATGT 1 cut(s) 480
PcsI WCGNNNNNNNCGW 1 cut(s) 1107
PctI GAATGC 1 cut(s) 79
PdmI GAANNNNTTC 2 cut(s) 950, 1164
PfeI GAWTC 5 cut(s) 170, 196, 268, 727, 835
Pfl23II CGTACG 1 cut(s) 314
PflMI CCANNNNNTGG 1 cut(s) 352
PleI GAGTC 1 cut(s) 323
PpsI GAGTC 1 cut(s) 323
Ppu21I YACGTR 1 cut(s) 131
PscI ACATGT 1 cut(s) 480
Psp124BI GAGCTC 1 cut(s) 972
Psp6I CCWGG 1 cut(s) 1240
PspGI CCWGG 1 cut(s) 1240
PspLI CGTACG 1 cut(s) 314
PspN4I GGNNCC 4 cut(s) 6, 15, 279, 1269
PspPI GGNCC 4 cut(s) 4, 1045, 1195, 1312
PstI CTGCAG 1 cut(s) 601
PstNI CAGNNNCTG 2 cut(s) 809, 1381
PsuI RGATCY 2 cut(s) 13, 1322
RsaI GTAC 6 cut(s) 316, 569, 720, 825, 864, 1329
RsaNI GTAC 6 cut(s) 315, 568, 719, 824, 863, 1328
RseI CAYNNNNRTG 1 cut(s) 474
SacI GAGCTC 1 cut(s) 972
SaqAI TTAA 6 cut(s) 153, 530, 749, 840, 1113, 1134
Sau3AI GATC 4 cut(s) 13, 631, 901, 1322
Sau96I GGNCC 4 cut(s) 4, 1045, 1195, 1312
SchI GAGTC 1 cut(s) 323
ScrFI CCNGG 1 cut(s) 1242
SduI GDGCHC 2 cut(s) 972, 1270
SfaNI GCATC 9 cut(s) 133, 155, 248, 529, 592, 796, 1093, 1141, 1387
SfcI CTRYAG 1 cut(s) 597
SinI GGWCC 4 cut(s) 4, 1045, 1195, 1312
SmiMI CAYNNNNRTG 1 cut(s) 474
SmlI CTYRAG 3 cut(s) 492, 1133, 1471
SmoI CTYRAG 3 cut(s) 492, 1133, 1471
SphI GCATGC 2 cut(s) 81, 1421
Sse9I AATT 9 cut(s) 435, 498, 527, 777, 894, 991, 1114, 1201, 1399
SsiI CCGC 1 cut(s) 292
SstI GAGCTC 1 cut(s) 972
StyD4I CCNGG 1 cut(s) 1240
StyI CCWWGG 2 cut(s) 829, 1318
TaaI ACNGT 5 cut(s) 314, 823, 939, 1370, 1456
TaiI ACGT 1 cut(s) 133
TaqI TCGA 3 cut(s) 168, 228, 577
TasI AATT 9 cut(s) 435, 498, 527, 777, 894, 991, 1114, 1201, 1399
TatI WGTACW 1 cut(s) 1327
TauI GCSGC 1 cut(s) 294
TfiI GAWTC 5 cut(s) 170, 196, 268, 727, 835
Tru1I TTAA 6 cut(s) 153, 530, 749, 840, 1113, 1134
Tru9I TTAA 6 cut(s) 153, 530, 749, 840, 1113, 1134
TscAI CASTG 2 cut(s) 288, 1162
TseFI GTSAC 1 cut(s) 772
Tsp45I GTSAC 1 cut(s) 772
TspDTI ATGAA 3 cut(s) 188, 787, 933
TspGWI ACGGA 7 cut(s) 218, 392, 475, 555, 801, 1208, 1347
TspRI CASTG 2 cut(s) 288, 1162
Van91I CCANNNNNTGG 1 cut(s) 352
Vha464I CTTAAG 1 cut(s) 1133
VpaK11BI GGWCC 4 cut(s) 4, 1045, 1195, 1312
XapI RAATTY 3 cut(s) 435, 991, 1201
XceI RCATGY 4 cut(s) 81, 484, 644, 1421
XmnI GAANNNNTTC 2 cut(s) 950, 1164
Zsp2I ATGCAT 2 cut(s) 79, 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.