pycom17g27080
ERF Family

Belongs to the GST superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0013890
Physical Location & Seq
Forward (+)
25355973 .. 25357297
1325 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 648 bp
ATGGTTGTCAAAGTATATGGTCCAGTAATGGCAGCCTGCCCCCAGAGGGTGATGGTTTGCCTTTTGGAGAAAGGAGTGAACTTTGAAATTGTAGATGTTGATCTTGAGGCAGGAGAGCACAAGCGACCCGAGTTTCTCACCCGTCAGCCGTTTGGGCAAGTTCCAGTTGTAGAAGATGGTGACTTAAGGCTCTTTGAATCTAGGGCCATCATAAGGTACTACGCAGCCAAGTATGCAGGCCGTGGTCCCAACCTATTGGGAACAACTCTGGAGGAGAAGGCAGTTGTCGACCAGTGGCTGGAAGTGGAAGGCCACAACTTCAACGACCTGGTTTACACTCTGGTGCTCCAGCTTCTTGTCCTGCCACGGATGGGTCAACGTGGCGACGTTGCATTAATCAATGCATGTGAGGAGAAGCTGGAGAAGGTGTTTGATGTTTATGAGGAAAGATTGTCAAAAAGCAAATATCTTGCTGGAGAAACTTTCACTCTGGCTGATCTGAGCCATCTTCCTGGGATTAGGTATCTGATTGATGACGCTAAACTGGGGCATTTGGTGACTGAGAGGAAGAAAGTGAATGCATGGTGGGAGGACATATCCAACAGGCCTTCTTGGAAGAAACTAATGCAGCTTGCTAGTGACTACTAG

Protein Analysis

216

Amino Acids

24.54

Weight (kDa)

5.5

Isoelectric Point (pI)

34.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 1 - 73 4.1e-20 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 10 - 81 1.6e-11 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 13 - 75 1.8e-13 Glutathione S-transferase, N-terminal domain
GST_C PF00043 115 - 203 8.6e-13 Glutathione S-transferase, C-terminal domain
GST_C_3 PF14497 136 - 206 2.7e-07 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013890)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03190 AT5G17220
fragaria_vesca FvH4_1g27460
malus_domestica MD17G1272100.v1.1
prunus_persica Prupe.3G013600_v2.0.a1
pyrus_communis pycom17g27080
rosa_chinensis RchiOBHm_Chr3g0488551
rosa_laevigata RLG00000022944
rosa_multiflora Rmu_co8213432.1_g000001 Rmu_sc0005363.1_g000020
rosa_roxburghii Rroxscaffold_6G00394180
rosa_rugosa Rorug03G0239200.1
rosa_samantha Rh3AG288000 Rh3BG324700 Rh3CG322100 Rh3DG320800
rosa_wichuraiana Rw3G025500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 298
AccI GTMKAC 1 cut(s) 288
AfaI GTAC 1 cut(s) 218
AfiI CCNNNNNNNGG 4 cut(s) 46, 213, 298, 371
AflII CTTAAG 1 cut(s) 184
AgsI TTSAA 3 cut(s) 86, 197, 322
AjnI CCWGG 2 cut(s) 327, 511
AleI CACNNNNGTG 1 cut(s) 341
AluBI AGCT 3 cut(s) 352, 418, 631
AluI AGCT 3 cut(s) 352, 418, 631
Alw21I GWGCWC 2 cut(s) 120, 348
AlwNI CAGNNNCTG 1 cut(s) 298
Ama87I CYCGRG 1 cut(s) 128
AoxI GGCC 4 cut(s) 204, 238, 310, 605
ApeKI GCWGC 3 cut(s) 32, 224, 628
AseI ATTAAT 1 cut(s) 395
AspS9I GGNCC 3 cut(s) 20, 204, 245
AsuHPI GGTGA 4 cut(s) 61, 130, 191, 568
AvaI CYCGRG 1 cut(s) 128
AvaII GGWCC 2 cut(s) 20, 245
Bbv12I GWGCWC 2 cut(s) 120, 348
BbvI GCAGC 3 cut(s) 44, 236, 640
BccI CCATC 5 cut(s) 46, 170, 215, 364, 513
BceAI ACGGC 2 cut(s) 133, 225
BciT130I CCWGG 2 cut(s) 329, 513
BfaI CTAG 3 cut(s) 201, 636, 646
BfrI CTTAAG 1 cut(s) 184
BglI GCCNNNNNGGC 1 cut(s) 154
BisI GCNGC 3 cut(s) 33, 225, 629
BlsI GCNGC 3 cut(s) 34, 226, 630
Bme1390I CCNGG 2 cut(s) 329, 513
Bme18I GGWCC 2 cut(s) 20, 245
BmeT110I CYCGRG 1 cut(s) 128
BmgT120I GGNCC 3 cut(s) 20, 204, 245
BmiI GGNNCC 1 cut(s) 247
BmrFI CCNGG 2 cut(s) 329, 513
BmrI ACTGGG 1 cut(s) 554
BmuI ACTGGG 1 cut(s) 554
BpmI CTGGAG 4 cut(s) 290, 332, 440, 495
BpuEI CTTGAG 1 cut(s) 125
BsaBI GATNNNNATC 1 cut(s) 99
BsaJI CCNNGG 3 cut(s) 241, 365, 512
BsaXI ACNNNNNCTCC 2 cut(s) 413, 443
Bsc4I CCNNNNNNNGG 4 cut(s) 46, 213, 298, 371
Bse1I ACTGG 4 cut(s) 23, 164, 292, 549
Bse8I GATNNNNATC 1 cut(s) 99
BseBI CCWGG 2 cut(s) 329, 513
BseDI CCNNGG 3 cut(s) 241, 365, 512
BseGI GGATG 1 cut(s) 375
BseJI GATNNNNATC 1 cut(s) 99
BseLI CCNNNNNNNGG 4 cut(s) 46, 213, 298, 371
BseMII CTCAG 2 cut(s) 491, 552
BseNI ACTGG 4 cut(s) 23, 164, 292, 549
BseRI GAGGAG 2 cut(s) 287, 425
BseXI GCAGC 3 cut(s) 44, 236, 640
BshFI GGCC 4 cut(s) 206, 240, 312, 607
BsiHKAI GWGCWC 2 cut(s) 120, 348
BsiHKCI CYCGRG 1 cut(s) 128
BslFI GGGAC 1 cut(s) 231
BslI CCNNNNNNNGG 4 cut(s) 46, 213, 298, 371
BsmFI GGGAC 1 cut(s) 231
BsmI GAATGC 1 cut(s) 583
BsnI GGCC 4 cut(s) 206, 240, 312, 607
BsoBI CYCGRG 1 cut(s) 128
Bsp1286I GDGCHC 2 cut(s) 120, 348
Bsp143I GATC 2 cut(s) 100, 496
BspANI GGCC 4 cut(s) 206, 240, 312, 607
BspCNI CTCAG 2 cut(s) 492, 553
BspLI GGNNCC 1 cut(s) 247
BspTI CTTAAG 1 cut(s) 184
BsrI ACTGG 4 cut(s) 23, 164, 292, 549
BssECI CCNNGG 3 cut(s) 241, 365, 512
BssMI GATC 2 cut(s) 100, 496
Bst2UI CCWGG 2 cut(s) 329, 513
BstAFI CTTAAG 1 cut(s) 184
BstC8I GCNNGC 3 cut(s) 37, 238, 633
BstDEI CTNAG 2 cut(s) 500, 561
BstDSI CCRYGG 2 cut(s) 241, 365
BstF5I GGATG 1 cut(s) 375
BstKTI GATC 2 cut(s) 103, 499
BstMBI GATC 2 cut(s) 100, 496
BstMWI GCNNNNNNNGC 2 cut(s) 154, 233
BstNI CCWGG 2 cut(s) 329, 513
BstNSI RCATGY 1 cut(s) 408
BstSCI CCNGG 2 cut(s) 327, 511
BstV1I GCAGC 3 cut(s) 44, 236, 640
BstXI CCANNNNNNTGG 2 cut(s) 256, 512
BsuRI GGCC 4 cut(s) 206, 240, 312, 607
BtgI CCRYGG 2 cut(s) 241, 365
BtsCI GGATG 1 cut(s) 375
BtsIMutI CAGTG 1 cut(s) 299
Cac8I GCNNGC 3 cut(s) 37, 238, 633
CaiI CAGNNNCTG 1 cut(s) 298
Cfr13I GGNCC 3 cut(s) 20, 204, 245
CseI GACGC 1 cut(s) 545
CsiI ACCWGGT 1 cut(s) 327
Csp6I GTAC 1 cut(s) 217
CviAII CATG 2 cut(s) 405, 582
CviQI GTAC 1 cut(s) 217
DdeI CTNAG 2 cut(s) 500, 561
DpnI GATC 2 cut(s) 102, 498
DpnII GATC 2 cut(s) 100, 496
Eco147I AGGCCT 1 cut(s) 607
Eco47I GGWCC 2 cut(s) 20, 245
Eco88I CYCGRG 1 cut(s) 128
EcoRII CCWGG 2 cut(s) 327, 511
EcoT22I ATGCAT 2 cut(s) 406, 583
FaeI CATG 2 cut(s) 408, 585
FaiI YATR 8 cut(s) 16, 18, 212, 234, 406, 441, 583, 596
FaqI GGGAC 1 cut(s) 231
FatI CATG 2 cut(s) 404, 581
FblI GTMKAC 1 cut(s) 288
Fnu4HI GCNGC 3 cut(s) 33, 225, 629
FokI GGATG 1 cut(s) 382
Fsp4HI GCNGC 3 cut(s) 33, 225, 629
FspBI CTAG 3 cut(s) 201, 636, 646
GluI GCNGC 3 cut(s) 33, 225, 629
GsuI CTGGAG 4 cut(s) 290, 332, 440, 495
HaeIII GGCC 4 cut(s) 206, 240, 312, 607
HgaI GACGC 1 cut(s) 545
Hin1II CATG 2 cut(s) 408, 585
HincII GTYRAC 2 cut(s) 289, 377
HindII GTYRAC 2 cut(s) 289, 377
HinfI GANTC 1 cut(s) 197
HphI GGTGA 4 cut(s) 61, 130, 191, 568
Hpy166II GTNNAC 4 cut(s) 79, 289, 334, 377
Hpy188I TCNGA 2 cut(s) 501, 528
Hpy188III TCNNGA 2 cut(s) 104, 269
Hpy8I GTNNAC 4 cut(s) 79, 289, 334, 377
Hpy99I CGWCG 1 cut(s) 389
HpyAV CCTTC 4 cut(s) 271, 302, 418, 618
HpyCH4IV ACGT 2 cut(s) 379, 387
HpyCH4V TGCA 5 cut(s) 236, 392, 404, 581, 628
HpyF10VI GCNNNNNNNGC 2 cut(s) 154, 233
HpyF3I CTNAG 2 cut(s) 500, 561
HpySE526I ACGT 2 cut(s) 379, 387
Hsp92II CATG 2 cut(s) 408, 585
Kzo9I GATC 2 cut(s) 100, 496
LmnI GCTCC 1 cut(s) 351
Lsp1109I GCAGC 3 cut(s) 44, 236, 640
MabI ACCWGGT 1 cut(s) 327
MaeI CTAG 3 cut(s) 201, 636, 646
MaeII ACGT 2 cut(s) 379, 387
MaeIII GTNAC 3 cut(s) 179, 556, 638
MalI GATC 2 cut(s) 102, 498
MboI GATC 2 cut(s) 100, 496
MboII GAAGA 4 cut(s) 185, 500, 580, 628
MhlI GDGCHC 2 cut(s) 120, 348
MluCI AATT 1 cut(s) 87
MmeI TCCRAC 1 cut(s) 624
MnlI CCTC 7 cut(s) 39, 100, 265, 403, 436, 558, 583
Mph1103I ATGCAT 2 cut(s) 406, 583
MseI TTAA 2 cut(s) 185, 395
MslI CAYNNNNRTG 1 cut(s) 341
MspCI CTTAAG 1 cut(s) 184
MspR9I CCNGG 2 cut(s) 329, 513
Mva1269I GAATGC 1 cut(s) 583
MvaI CCWGG 2 cut(s) 329, 513
MwoI GCNNNNNNNGC 2 cut(s) 154, 233
NdeII GATC 2 cut(s) 100, 496
NlaIII CATG 2 cut(s) 408, 585
NlaIV GGNNCC 1 cut(s) 247
NmuCI GTSAC 3 cut(s) 179, 556, 638
NsiI ATGCAT 2 cut(s) 406, 583
NspI RCATGY 1 cut(s) 408
OliI CACNNNNGTG 1 cut(s) 341
PceI AGGCCT 1 cut(s) 607
PctI GAATGC 1 cut(s) 583
PfeI GAWTC 1 cut(s) 197
PflMI CCANNNNNTGG 1 cut(s) 298
PkrI GCNGC 3 cut(s) 34, 226, 630
PshBI ATTAAT 1 cut(s) 395
Psp6I CCWGG 2 cut(s) 327, 511
PspGI CCWGG 2 cut(s) 327, 511
PspN4I GGNNCC 1 cut(s) 247
PspPI GGNCC 3 cut(s) 20, 204, 245
PstNI CAGNNNCTG 1 cut(s) 298
RsaI GTAC 1 cut(s) 218
RsaNI GTAC 1 cut(s) 217
RseI CAYNNNNRTG 1 cut(s) 341
SalI GTCGAC 1 cut(s) 287
SaqAI TTAA 2 cut(s) 185, 395
SatI GCNGC 3 cut(s) 33, 225, 629
Sau3AI GATC 2 cut(s) 100, 496
Sau96I GGNCC 3 cut(s) 20, 204, 245
ScrFI CCNGG 2 cut(s) 329, 513
SduI GDGCHC 2 cut(s) 120, 348
SexAI ACCWGGT 1 cut(s) 327
SinI GGWCC 2 cut(s) 20, 245
SmiMI CAYNNNNRTG 1 cut(s) 341
SmlI CTYRAG 2 cut(s) 104, 184
SmoI CTYRAG 2 cut(s) 104, 184
Sse9I AATT 1 cut(s) 87
SseBI AGGCCT 1 cut(s) 607
SspMI CTAG 3 cut(s) 201, 636, 646
StuI AGGCCT 1 cut(s) 607
StyD4I CCNGG 2 cut(s) 327, 511
TaiI ACGT 2 cut(s) 382, 390
TaqI TCGA 1 cut(s) 288
TasI AATT 1 cut(s) 87
TfiI GAWTC 1 cut(s) 197
Tru1I TTAA 2 cut(s) 185, 395
Tru9I TTAA 2 cut(s) 185, 395
TscAI CASTG 1 cut(s) 299
TseFI GTSAC 3 cut(s) 179, 556, 638
TseI GCWGC 3 cut(s) 32, 224, 628
Tsp45I GTSAC 3 cut(s) 179, 556, 638
TspGWI ACGGA 1 cut(s) 382
TspRI CASTG 1 cut(s) 299
Van91I CCANNNNNTGG 1 cut(s) 298
Vha464I CTTAAG 1 cut(s) 184
VpaK11BI GGWCC 2 cut(s) 20, 245
VspI ATTAAT 1 cut(s) 395
XceI RCATGY 1 cut(s) 408
XmiI GTMKAC 1 cut(s) 288
XspI CTAG 3 cut(s) 201, 636, 646
Zsp2I ATGCAT 2 cut(s) 406, 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.