pycom461g00180

ATP synthesis coupled electron transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000461
OG0010702
Physical Location & Seq
Forward (+)
122758 .. 124880
2123 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 756 bp
ATGTGGGCACCTGATATCTATGAGGGTTCACCCACCCCGGTTACAGCATTCCTTTCTATTGCGCCTAAAATATCTATTTCTGCAAATATTTCACGTGTTTCTATTTATGGTTCCTATGGAGCTACATTGCAACAAATCTTCTTTTTCTGCAGCATTGCTTCTATGATCTTAGGAGCACTGGCCGCCATGGCCCAAACGAAAGTAAAAAGACCTCTAGCTCATAGTTCAATTGGACATGTAGGTTATATTCGTACAGGTTTATCATGTGGAACCATAGAAGGAATTCAATCACTACTAATTGGTATCTTTATTTATGCATTAATGACGATAGATGCATTCGCCATAGTTTTAGCATTACGGCAAACCCGTGTCAAATATATAGCGGATTTGGGCGCTCTAGCCAAAACGAATCCTATTTCGGCTATTACCTTCTCCATTACTATGTTCTCATACGCAGGAATACCCCCGTTAGCCGGCTTTTGTAGCAAATTCTATTTGTTCTTCGCCGCTTTGGGTTGTGGGGCTTACTTCCTAGCCCCAGTGGGAGTAGTGACTAGCGTTATAGGTCTTTATTATATACGCTTAGCGAAAAGAATGTTTTTTGATACACCTAGGACATGGATTCTATATGAACCAATGGATCGTGACAAGTCGTTACTACTAGCAATGACTTCCTCTTTCATTACTTCATCCTTTTCATATCCCTCTCCCTTGTTCTCAGTTACTCATCAAATGGCACTCAGTTCATATCTTTAA

Protein Analysis

252

Amino Acids

27.3

Weight (kDa)

9.37

Isoelectric Point (pI)

31.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proton_antipo_M PF00361 1 - 189 1.6e-53 NADH:quinone oxidoreductase/Mrp antiporter, TM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 7
AciI CCGC 3 cut(s) 183, 383, 507
AclWI GGATC 1 cut(s) 648
AcoI YGGCCR 1 cut(s) 180
AcsI RAATTY 2 cut(s) 282, 488
AcvI CACGTG 1 cut(s) 95
AfaI GTAC 1 cut(s) 253
AfiI CCNNNNNNNGG 1 cut(s) 473
AflIII ACRYGT 2 cut(s) 94, 235
AgsI TTSAA 2 cut(s) 228, 287
AluBI AGCT 2 cut(s) 122, 218
AluI AGCT 2 cut(s) 122, 218
Alw21I GWGCWC 1 cut(s) 178
AlwI GGATC 1 cut(s) 648
AoxI GGCC 2 cut(s) 180, 189
ApeKI GCWGC 1 cut(s) 150
ApoI RAATTY 2 cut(s) 282, 488
AseI ATTAAT 1 cut(s) 320
Asp700I GAANNNNTTC 1 cut(s) 282
AspA2I CCTAGG 1 cut(s) 611
AspLEI GCGC 2 cut(s) 64, 395
AspS9I GGNCC 1 cut(s) 190
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 1 cut(s) 21
AvrII CCTAGG 1 cut(s) 611
BaeGI GKGCMC 1 cut(s) 10
BanI GGYRCC 1 cut(s) 7
BbrPI CACGTG 1 cut(s) 95
Bbv12I GWGCWC 1 cut(s) 178
BbvI GCAGC 1 cut(s) 162
BceAI ACGGC 1 cut(s) 374
BcnI CCSGG 1 cut(s) 38
BfaI CTAG 6 cut(s) 215, 398, 533, 555, 612, 662
BfmI CTRYAG 1 cut(s) 148
BfoI RGCGCY 1 cut(s) 396
BglI GCCNNNNNGGC 1 cut(s) 188
BisI GCNGC 3 cut(s) 151, 183, 507
BlnI CCTAGG 1 cut(s) 611
BlpI GCTNAGC 1 cut(s) 583
BlsI GCNGC 3 cut(s) 152, 184, 508
Bme1390I CCNGG 1 cut(s) 38
BmgT120I GGNCC 1 cut(s) 190
BmiI GGNNCC 3 cut(s) 9, 112, 271
BmrFI CCNGG 1 cut(s) 38
BmrI ACTGGG 1 cut(s) 533
BmsI GCATC 1 cut(s) 322
BmuI ACTGGG 1 cut(s) 533
Bpu1102I GCTNAGC 1 cut(s) 583
BpuMI CCSGG 1 cut(s) 38
BsaAI YACGTR 1 cut(s) 95
BsaJI CCNNGG 3 cut(s) 36, 186, 611
Bsc4I CCNNNNNNNGG 1 cut(s) 473
Bse118I RCCGGY 1 cut(s) 473
Bse1I ACTGG 2 cut(s) 183, 539
Bse3DI GCAATG 3 cut(s) 125, 153, 672
BseDI CCNNGG 3 cut(s) 36, 186, 611
BseGI GGATG 1 cut(s) 689
BseLI CCNNNNNNNGG 1 cut(s) 473
BseMI GCAATG 3 cut(s) 125, 153, 672
BseMII CTCAG 2 cut(s) 732, 754
BseNI ACTGG 2 cut(s) 183, 539
BseSI GKGCMC 1 cut(s) 10
BseXI GCAGC 1 cut(s) 162
BshFI GGCC 2 cut(s) 182, 191
BshNI GGYRCC 1 cut(s) 7
BsiHKAI GWGCWC 1 cut(s) 178
BsiSI CCGG 2 cut(s) 38, 474
BslI CCNNNNNNNGG 1 cut(s) 473
BsmI GAATGC 2 cut(s) 47, 335
BsnI GGCC 2 cut(s) 182, 191
Bsp1286I GDGCHC 2 cut(s) 10, 178
Bsp143I GATC 2 cut(s) 165, 640
Bsp1720I GCTNAGC 1 cut(s) 583
Bsp19I CCATGG 1 cut(s) 186
BspACI CCGC 3 cut(s) 183, 383, 507
BspANI GGCC 2 cut(s) 182, 191
BspCNI CTCAG 2 cut(s) 731, 753
BspLI GGNNCC 3 cut(s) 9, 112, 271
BspMAI CTGCAG 1 cut(s) 152
BspPI GGATC 1 cut(s) 648
BspT107I GGYRCC 1 cut(s) 7
BsrDI GCAATG 3 cut(s) 125, 153, 672
BsrFI RCCGGY 1 cut(s) 473
BsrI ACTGG 2 cut(s) 183, 539
BssAI RCCGGY 1 cut(s) 473
BssECI CCNNGG 3 cut(s) 36, 186, 611
BssMI GATC 2 cut(s) 165, 640
BssT1I CCWWGG 2 cut(s) 186, 611
BstBAI YACGTR 1 cut(s) 95
BstC8I GCNNGC 1 cut(s) 475
BstDEI CTNAG 4 cut(s) 169, 583, 718, 740
BstDSI CCRYGG 1 cut(s) 186
BstF5I GGATG 1 cut(s) 689
BstH2I RGCGCY 1 cut(s) 396
BstHHI GCGC 2 cut(s) 64, 395
BstKTI GATC 2 cut(s) 168, 643
BstMBI GATC 2 cut(s) 165, 640
BstMWI GCNNNNNNNGC 3 cut(s) 182, 188, 483
BstNSI RCATGY 1 cut(s) 239
BstSCI CCNGG 1 cut(s) 36
BstSFI CTRYAG 1 cut(s) 148
BstSLI GKGCMC 1 cut(s) 10
BstV1I GCAGC 1 cut(s) 162
BsuRI GGCC 2 cut(s) 182, 191
BtgI CCRYGG 1 cut(s) 186
BtsCI GGATG 1 cut(s) 689
BtsIMutI CAGTG 2 cut(s) 176, 546
Cac8I GCNNGC 1 cut(s) 475
CfoI GCGC 2 cut(s) 64, 395
Cfr10I RCCGGY 1 cut(s) 473
Cfr13I GGNCC 1 cut(s) 190
Csp6I GTAC 1 cut(s) 252
CviAII CATG 4 cut(s) 187, 236, 264, 618
CviQI GTAC 1 cut(s) 252
DdeI CTNAG 4 cut(s) 169, 583, 718, 740
DpnI GATC 2 cut(s) 167, 642
DpnII GATC 2 cut(s) 165, 640
EaeI YGGCCR 1 cut(s) 180
Eco130I CCWWGG 2 cut(s) 186, 611
Eco32I GATATC 1 cut(s) 16
Eco72I CACGTG 1 cut(s) 95
EcoRI GAATTC 1 cut(s) 282
EcoRV GATATC 1 cut(s) 16
EcoT14I CCWWGG 2 cut(s) 186, 611
EcoT22I ATGCAT 2 cut(s) 319, 337
ErhI CCWWGG 2 cut(s) 186, 611
FaeI CATG 4 cut(s) 190, 239, 267, 621
FatI CATG 4 cut(s) 186, 235, 263, 617
Fnu4HI GCNGC 3 cut(s) 151, 183, 507
FokI GGATG 1 cut(s) 676
Fsp4HI GCNGC 3 cut(s) 151, 183, 507
FspBI CTAG 6 cut(s) 215, 398, 533, 555, 612, 662
GlaI GCGC 2 cut(s) 63, 394
GluI GCNGC 3 cut(s) 151, 183, 507
HaeII RGCGCY 1 cut(s) 396
HaeIII GGCC 2 cut(s) 182, 191
HapII CCGG 2 cut(s) 38, 474
HhaI GCGC 2 cut(s) 64, 395
Hin1II CATG 4 cut(s) 190, 239, 267, 621
Hin6I GCGC 2 cut(s) 62, 393
HinP1I GCGC 2 cut(s) 62, 393
HinfI GANTC 2 cut(s) 409, 622
HpaII CCGG 2 cut(s) 38, 474
HphI GGTGA 1 cut(s) 21
Hpy166II GTNNAC 1 cut(s) 29
Hpy188III TCNNGA 1 cut(s) 644
Hpy8I GTNNAC 1 cut(s) 29
HpyAV CCTTC 2 cut(s) 272, 439
HpyCH4IV ACGT 1 cut(s) 94
HpyCH4V TGCA 5 cut(s) 83, 130, 150, 317, 335
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 188, 483
HpyF3I CTNAG 4 cut(s) 169, 583, 718, 740
HpySE526I ACGT 1 cut(s) 94
Hsp92II CATG 4 cut(s) 190, 239, 267, 621
HspAI GCGC 2 cut(s) 62, 393
KroI GCCGGC 1 cut(s) 473
KroNI GCCGGC 1 cut(s) 475
Kzo9I GATC 2 cut(s) 165, 640
LmnI GCTCC 2 cut(s) 119, 173
LpnPI CCDG 7 cut(s) 24, 51, 164, 240, 441, 487, 552
Lsp1109I GCAGC 1 cut(s) 162
LweI GCATC 1 cut(s) 322
MaeI CTAG 6 cut(s) 215, 398, 533, 555, 612, 662
MaeII ACGT 1 cut(s) 94
MaeIII GTNAC 5 cut(s) 40, 550, 644, 654, 721
MalI GATC 2 cut(s) 167, 642
MboI GATC 2 cut(s) 165, 640
MboII GAAGA 2 cut(s) 130, 493
MfeI CAATTG 1 cut(s) 228
MhlI GDGCHC 2 cut(s) 10, 178
MluCI AATT 4 cut(s) 228, 282, 297, 488
MnlI CCTC 4 cut(s) 16, 222, 685, 715
Mph1103I ATGCAT 2 cut(s) 319, 337
MroNI GCCGGC 1 cut(s) 473
MroXI GAANNNNTTC 1 cut(s) 282
MseI TTAA 2 cut(s) 320, 754
MslI CAYNNNNRTG 1 cut(s) 440
MspI CCGG 2 cut(s) 38, 474
MspR9I CCNGG 1 cut(s) 38
MunI CAATTG 1 cut(s) 228
Mva1269I GAATGC 2 cut(s) 47, 335
MwoI GCNNNNNNNGC 3 cut(s) 182, 188, 483
NaeI GCCGGC 1 cut(s) 475
NciI CCSGG 1 cut(s) 38
NcoI CCATGG 1 cut(s) 186
NdeII GATC 2 cut(s) 165, 640
NgoMIV GCCGGC 1 cut(s) 473
NlaIII CATG 4 cut(s) 190, 239, 267, 621
NlaIV GGNNCC 3 cut(s) 9, 112, 271
NmuCI GTSAC 2 cut(s) 550, 644
NsiI ATGCAT 2 cut(s) 319, 337
NspI RCATGY 1 cut(s) 239
PciI ACATGT 1 cut(s) 235
PcsI WCGNNNNNNNCGW 1 cut(s) 364
PctI GAATGC 2 cut(s) 47, 335
PdiI GCCGGC 1 cut(s) 475
PdmI GAANNNNTTC 1 cut(s) 282
PfeI GAWTC 2 cut(s) 409, 622
PkrI GCNGC 3 cut(s) 152, 184, 508
PmaCI CACGTG 1 cut(s) 95
PmlI CACGTG 1 cut(s) 95
Ppu21I YACGTR 1 cut(s) 95
PscI ACATGT 1 cut(s) 235
PshBI ATTAAT 1 cut(s) 320
PspCI CACGTG 1 cut(s) 95
PspN4I GGNNCC 3 cut(s) 9, 112, 271
PspPI GGNCC 1 cut(s) 190
PstI CTGCAG 1 cut(s) 152
RsaI GTAC 1 cut(s) 253
RsaNI GTAC 1 cut(s) 252
RseI CAYNNNNRTG 1 cut(s) 440
SaqAI TTAA 2 cut(s) 320, 754
SatI GCNGC 3 cut(s) 151, 183, 507
Sau3AI GATC 2 cut(s) 165, 640
Sau96I GGNCC 1 cut(s) 190
ScrFI CCNGG 1 cut(s) 38
SduI GDGCHC 2 cut(s) 10, 178
SfaNI GCATC 1 cut(s) 322
SfcI CTRYAG 1 cut(s) 148
SfiI GGCCNNNNNGGCC 1 cut(s) 188
SmiMI CAYNNNNRTG 1 cut(s) 440
Sse9I AATT 4 cut(s) 228, 282, 297, 488
SsiI CCGC 3 cut(s) 183, 383, 507
SspI AATATT 1 cut(s) 88
SspMI CTAG 6 cut(s) 215, 398, 533, 555, 612, 662
StyD4I CCNGG 1 cut(s) 36
StyI CCWWGG 2 cut(s) 186, 611
TaiI ACGT 1 cut(s) 97
TasI AATT 4 cut(s) 228, 282, 297, 488
TauI GCSGC 2 cut(s) 185, 509
TfiI GAWTC 2 cut(s) 409, 622
Tru1I TTAA 2 cut(s) 320, 754
Tru9I TTAA 2 cut(s) 320, 754
TscAI CASTG 2 cut(s) 183, 546
TseFI GTSAC 2 cut(s) 550, 644
TseI GCWGC 1 cut(s) 150
Tsp45I GTSAC 2 cut(s) 550, 644
TspDTI ATGAA 5 cut(s) 645, 670, 678, 687, 735
TspRI CASTG 2 cut(s) 183, 546
VspI ATTAAT 1 cut(s) 320
XapI RAATTY 2 cut(s) 282, 488
XceI RCATGY 1 cut(s) 239
XmaJI CCTAGG 1 cut(s) 611
XmnI GAANNNNTTC 1 cut(s) 282
XspI CTAG 6 cut(s) 215, 398, 533, 555, 612, 662
Zsp2I ATGCAT 2 cut(s) 319, 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.