RCHIOBHM_MTG0498511

Proton-conducting membrane transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
Mt
OG0010702
Physical Location & Seq
Forward (+)
91150 .. 92753
1604 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15618

Sequence Viewer

Length: 1089 bp
ATGATTGCCGGGCCCTCTCCTCGTTCCGCCCGCCGGCCTATTGGGATAGCAGCCTTAGGGCTTTGCCTGCCCTTTAAAAAAGGCTCGGCCCGGGAAAGCGCTGGCAACAACAGAAAGGAAGGGGTCCATGTAGCTGCTGCGCCCGCCCCCTTCTTAGTCAATGGGGCAGCAGGTTCGGCATCTACTATAAAAGAGAGAATCCGCTTTCTTTCAGAACCTCTGCTAGGCAGCGTAGTGGAGTTACGGGAACAGCCGTCTGATGGAAAACAACTTTCACGTTCGGTTCAGAGAGCACTTTTTTCGTTGAGAATTCCTCGTTCCCTTTCGTGTGAATTCCCCTGCAACGAATTTCAAACTTGCGGGGCCCTATCTATTTATTCCCTCTCTCGAGCCCCGAAGAAAACCCCCTCCCCTTCGGACTCCACATCTCTTTTGACTCTATATATGTGGGCACCTGATATCTATGAGGGTTCACCCACCCCGGTTACAGCATTCCTTTCTATTGCGCCTAAAATTTCTATTTCTGCTAATATTTCACGCGTTTCTATTTATGGTTCCTATGGAGCTACATTGCAACAAATCTTCTTTTTCTGCAGCATTGCTTCTATGATCTTAGGAGCACTGGCCGCCATGGCCCAAACGAAAGTAAAAAGACCTCTAGCTCATAGTTCAATTGGACATGTAGGTTATATTCGTACAGGTTTATCATGTGGAACCATAGAAGGAATTCAATCACTACTAATTGGTCTCTTTATTTATGCATTAATGACGATAGATGCATTCGCCATAGTTTTAGCATTACGGCAAACCCGTGTCAAATATATAGCGGATTTGGGCGCTTTAGCCAAAACGAATCCTATTTCGGCTATTACCTTCGCCATTACTATGTTCTCATACGCAGGAATACCCCCGTTAGCCGGCTTTTGTAGCAAATTCTATTTGTTCTTTGCCGCTTTGGGTTGTGGGGCTTACTTCCTAGCCCCAGTGGGAGTAGTGACTAGCGTTATAGGTCGTTGGGCGGCCGGAAGGTTGCCACGAGTAAGTAAGTTTGGGGGACCGAAGGTAGTTCTCCGTGCACCGGACACGTAG

Protein Analysis

362

Amino Acids

38.41

Weight (kDa)

9.97

Isoelectric Point (pI)

43.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proton_antipo_M PF00361 143 - 337 3.8e-54 NADH:quinone oxidoreductase/Mrp antiporter, TM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 161
AccB1I GGYRCC 1 cut(s) 451
AccII CGCG 1 cut(s) 540
AciI CCGC 9 cut(s) 27, 31, 144, 202, 360, 627, 827, 951, 1019
AcoI YGGCCR 2 cut(s) 624, 1020
AcsI RAATTY 6 cut(s) 309, 332, 347, 513, 726, 932
AfaI GTAC 1 cut(s) 697
AfeI AGCGCT 1 cut(s) 100
AfiI CCNNNNNNNGG 5 cut(s) 33, 224, 260, 917, 1078
AflIII ACRYGT 3 cut(s) 538, 679, 1083
AgsI TTSAA 3 cut(s) 353, 672, 731
AluBI AGCT 3 cut(s) 134, 566, 662
AluI AGCT 3 cut(s) 134, 566, 662
Alw21I GWGCWC 3 cut(s) 295, 622, 1078
Alw26I GTCTC 1 cut(s) 752
Alw44I GTGCAC 1 cut(s) 1074
Ama87I CYCGRG 2 cut(s) 90, 387
Aor51HI AGCGCT 1 cut(s) 100
AoxI GGCC 7 cut(s) 11, 35, 87, 363, 624, 633, 1020
ApaI GGGCCC 2 cut(s) 15, 367
ApaLI GTGCAC 1 cut(s) 1074
ApeKI GCWGC 6 cut(s) 50, 134, 137, 167, 228, 594
ApoI RAATTY 6 cut(s) 309, 332, 347, 513, 726, 932
AseI ATTAAT 1 cut(s) 764
Asp700I GAANNNNTTC 1 cut(s) 726
AspLEI GCGC 4 cut(s) 101, 142, 508, 839
AspS9I GGNCC 8 cut(s) 11, 12, 88, 124, 363, 364, 634, 1055
AsuC2I CCSGG 4 cut(s) 10, 91, 92, 482
AsuHPI GGTGA 1 cut(s) 465
AvaI CYCGRG 2 cut(s) 90, 387
AvaII GGWCC 2 cut(s) 124, 1055
AxyI CCTNAGG 1 cut(s) 55
BaeGI GKGCMC 4 cut(s) 15, 367, 454, 1078
BanI GGYRCC 1 cut(s) 451
BanII GRGCYC 3 cut(s) 15, 367, 394
BauI CACGAG 1 cut(s) 1035
Bbv12I GWGCWC 3 cut(s) 295, 622, 1078
BbvI GCAGC 6 cut(s) 62, 121, 124, 179, 240, 606
BccI CCATC 1 cut(s) 254
BceAI ACGGC 2 cut(s) 238, 818
BcgI CGANNNNNNTGC 4 cut(s) 156, 190, 282, 316
BcnI CCSGG 4 cut(s) 10, 91, 92, 482
BcoDI GTCTC 1 cut(s) 752
BfaI CTAG 4 cut(s) 224, 659, 977, 999
BfmI CTRYAG 1 cut(s) 592
BfoI RGCGCY 2 cut(s) 102, 840
BfuAI ACCTGC 1 cut(s) 161
BglI GCCNNNNNGGC 1 cut(s) 632
BisI GCNGC 9 cut(s) 51, 135, 138, 168, 229, 595, 627, 951, 1020
BlsI GCNGC 9 cut(s) 52, 136, 139, 169, 230, 596, 628, 952, 1021
Bme1390I CCNGG 4 cut(s) 10, 91, 92, 482
Bme18I GGWCC 2 cut(s) 124, 1055
BmeT110I CYCGRG 2 cut(s) 90, 387
BmgT120I GGNCC 8 cut(s) 11, 12, 88, 124, 363, 364, 634, 1055
BmiI GGNNCC 8 cut(s) 13, 125, 364, 365, 453, 556, 715, 1056
BmrFI CCNGG 4 cut(s) 10, 91, 92, 482
BmrI ACTGGG 1 cut(s) 977
BmsI GCATC 2 cut(s) 188, 766
BmuI ACTGGG 1 cut(s) 977
BplI GAGNNNNNCTC 2 cut(s) 298, 330
BpuMI CCSGG 4 cut(s) 10, 91, 92, 482
BsaAI YACGTR 1 cut(s) 1086
BsaI GGTCTC 1 cut(s) 752
BsaJI CCNNGG 3 cut(s) 90, 480, 630
BsaWI WCCGGW 1 cut(s) 1078
Bsc4I CCNNNNNNNGG 5 cut(s) 33, 224, 260, 917, 1078
Bse118I RCCGGY 2 cut(s) 33, 917
Bse1I ACTGG 2 cut(s) 627, 983
Bse21I CCTNAGG 1 cut(s) 55
Bse3DI GCAATG 2 cut(s) 569, 597
BseDI CCNNGG 3 cut(s) 90, 480, 630
BseLI CCNNNNNNNGG 5 cut(s) 33, 224, 260, 917, 1078
BseMI GCAATG 2 cut(s) 569, 597
BseNI ACTGG 2 cut(s) 627, 983
BseRI GAGGAG 1 cut(s) 9
BseSI GKGCMC 4 cut(s) 15, 367, 454, 1078
BseX3I CGGCCG 1 cut(s) 1020
BseXI GCAGC 6 cut(s) 62, 121, 124, 179, 240, 606
Bsh1236I CGCG 1 cut(s) 540
Bsh1285I CGRYCG 1 cut(s) 1023
BshFI GGCC 7 cut(s) 13, 37, 89, 365, 626, 635, 1022
BshNI GGYRCC 1 cut(s) 451
BsiEI CGRYCG 1 cut(s) 1023
BsiHKAI GWGCWC 3 cut(s) 295, 622, 1078
BsiHKCI CYCGRG 2 cut(s) 90, 387
BsiSI CCGG 7 cut(s) 9, 34, 91, 482, 918, 1023, 1079
BslFI GGGAC 1 cut(s) 1068
BslI CCNNNNNNNGG 5 cut(s) 33, 224, 260, 917, 1078
BsmAI GTCTC 1 cut(s) 752
BsmFI GGGAC 1 cut(s) 1068
BsmI GAATGC 2 cut(s) 491, 779
BsnI GGCC 7 cut(s) 13, 37, 89, 365, 626, 635, 1022
Bso31I GGTCTC 1 cut(s) 752
BsoBI CYCGRG 2 cut(s) 90, 387
Bsp120I GGGCCC 2 cut(s) 11, 363
Bsp1286I GDGCHC 7 cut(s) 15, 295, 367, 394, 454, 622, 1078
Bsp143I GATC 1 cut(s) 609
Bsp19I CCATGG 1 cut(s) 630
BspACI CCGC 9 cut(s) 27, 31, 144, 202, 360, 627, 827, 951, 1019
BspANI GGCC 7 cut(s) 13, 37, 89, 365, 626, 635, 1022
BspFNI CGCG 1 cut(s) 540
BspLI GGNNCC 8 cut(s) 13, 125, 364, 365, 453, 556, 715, 1056
BspMAI CTGCAG 1 cut(s) 596
BspMI ACCTGC 1 cut(s) 161
BspT107I GGYRCC 1 cut(s) 451
BspTNI GGTCTC 1 cut(s) 752
BsrDI GCAATG 2 cut(s) 569, 597
BsrFI RCCGGY 2 cut(s) 33, 917
BsrI ACTGG 2 cut(s) 627, 983
BssAI RCCGGY 2 cut(s) 33, 917
BssECI CCNNGG 3 cut(s) 90, 480, 630
BssMI GATC 1 cut(s) 609
BssSI CACGAG 1 cut(s) 1035
BssT1I CCWWGG 1 cut(s) 630
Bst2BI CACGAG 1 cut(s) 1035
BstBAI YACGTR 1 cut(s) 1086
BstC8I GCNNGC 6 cut(s) 31, 35, 68, 103, 144, 919
BstDEI CTNAG 3 cut(s) 55, 154, 613
BstDSI CCRYGG 1 cut(s) 630
BstENI CCTNNNNNAGG 1 cut(s) 222
BstFNI CGCG 1 cut(s) 540
BstH2I RGCGCY 2 cut(s) 102, 840
BstHHI GCGC 4 cut(s) 101, 142, 508, 839
BstKTI GATC 1 cut(s) 612
BstMAI GTCTC 1 cut(s) 752
BstMBI GATC 1 cut(s) 609
BstMCI CGRYCG 1 cut(s) 1023
BstMWI GCNNNNNNNGC 6 cut(s) 67, 143, 176, 626, 632, 927
BstNSI RCATGY 1 cut(s) 683
BstSCI CCNGG 4 cut(s) 8, 89, 90, 480
BstSFI CTRYAG 1 cut(s) 592
BstSLI GKGCMC 4 cut(s) 15, 367, 454, 1078
BstUI CGCG 1 cut(s) 540
BstV1I GCAGC 6 cut(s) 62, 121, 124, 179, 240, 606
BstZI CGGCCG 1 cut(s) 1020
Bsu36I CCTNAGG 1 cut(s) 55
BsuRI GGCC 7 cut(s) 13, 37, 89, 365, 626, 635, 1022
BtgI CCRYGG 1 cut(s) 630
BtsIMutI CAGTG 2 cut(s) 620, 990
BveI ACCTGC 1 cut(s) 161
Cac8I GCNNGC 6 cut(s) 31, 35, 68, 103, 144, 919
CfoI GCGC 4 cut(s) 101, 142, 508, 839
Cfr10I RCCGGY 2 cut(s) 33, 917
Cfr13I GGNCC 8 cut(s) 11, 12, 88, 124, 363, 364, 634, 1055
Cfr9I CCCGGG 1 cut(s) 90
Csp6I GTAC 1 cut(s) 696
CviAII CATG 4 cut(s) 128, 631, 680, 708
CviQI GTAC 1 cut(s) 696
DdeI CTNAG 3 cut(s) 55, 154, 613
DpnI GATC 1 cut(s) 611
DpnII GATC 1 cut(s) 609
DraI TTTAAA 1 cut(s) 76
EaeI YGGCCR 2 cut(s) 624, 1020
EagI CGGCCG 1 cut(s) 1020
EciI GGCGGA 1 cut(s) 16
EclXI CGGCCG 1 cut(s) 1020
Eco130I CCWWGG 1 cut(s) 630
Eco24I GRGCYC 3 cut(s) 15, 367, 394
Eco31I GGTCTC 1 cut(s) 752
Eco32I GATATC 1 cut(s) 460
Eco47I GGWCC 2 cut(s) 124, 1055
Eco47III AGCGCT 1 cut(s) 100
Eco52I CGGCCG 1 cut(s) 1020
Eco81I CCTNAGG 1 cut(s) 55
Eco88I CYCGRG 2 cut(s) 90, 387
EcoNI CCTNNNNNAGG 1 cut(s) 222
EcoO109I RGGNCCY 3 cut(s) 12, 363, 364
EcoRI GAATTC 3 cut(s) 309, 332, 726
EcoRV GATATC 1 cut(s) 460
EcoT14I CCWWGG 1 cut(s) 630
EcoT22I ATGCAT 2 cut(s) 763, 781
EcoT38I GRGCYC 3 cut(s) 15, 367, 394
ErhI CCWWGG 1 cut(s) 630
FaeI CATG 4 cut(s) 131, 634, 683, 711
FaqI GGGAC 1 cut(s) 1068
FatI CATG 4 cut(s) 127, 630, 679, 707
FauI CCCGC 3 cut(s) 38, 151, 353
Fnu4HI GCNGC 9 cut(s) 51, 135, 138, 168, 229, 595, 627, 951, 1020
FriOI GRGCYC 3 cut(s) 15, 367, 394
Fsp4HI GCNGC 9 cut(s) 51, 135, 138, 168, 229, 595, 627, 951, 1020
FspBI CTAG 4 cut(s) 224, 659, 977, 999
GlaI GCGC 4 cut(s) 100, 141, 507, 838
GluI GCNGC 9 cut(s) 51, 135, 138, 168, 229, 595, 627, 951, 1020
HaeII RGCGCY 2 cut(s) 102, 840
HaeIII GGCC 7 cut(s) 13, 37, 89, 365, 626, 635, 1022
HapII CCGG 7 cut(s) 9, 34, 91, 482, 918, 1023, 1079
HhaI GCGC 4 cut(s) 101, 142, 508, 839
Hin1II CATG 4 cut(s) 131, 634, 683, 711
Hin6I GCGC 4 cut(s) 99, 140, 506, 837
HinP1I GCGC 4 cut(s) 99, 140, 506, 837
HinfI GANTC 4 cut(s) 198, 419, 436, 853
HpaII CCGG 7 cut(s) 9, 34, 91, 482, 918, 1023, 1079
HphI GGTGA 1 cut(s) 465
Hpy166II GTNNAC 2 cut(s) 473, 1076
Hpy188I TCNGA 4 cut(s) 214, 259, 288, 418
Hpy188III TCNNGA 1 cut(s) 387
Hpy8I GTNNAC 2 cut(s) 473, 1076
HpyAV CCTTC 7 cut(s) 113, 160, 423, 716, 883, 1020, 1054
HpyCH4IV ACGT 2 cut(s) 277, 1085
HpyCH4V TGCA 6 cut(s) 342, 574, 594, 761, 779, 1076
HpyF10VI GCNNNNNNNGC 6 cut(s) 67, 143, 176, 626, 632, 927
HpyF3I CTNAG 3 cut(s) 55, 154, 613
HpySE526I ACGT 2 cut(s) 277, 1085
Hsp92II CATG 4 cut(s) 131, 634, 683, 711
HspAI GCGC 4 cut(s) 99, 140, 506, 837
KroI GCCGGC 2 cut(s) 33, 917
KroNI GCCGGC 2 cut(s) 35, 919
Kzo9I GATC 1 cut(s) 609
LmnI GCTCC 2 cut(s) 563, 617
Lsp1109I GCAGC 6 cut(s) 62, 121, 124, 179, 240, 606
LweI GCATC 2 cut(s) 188, 766
MaeI CTAG 4 cut(s) 224, 659, 977, 999
MaeII ACGT 2 cut(s) 277, 1085
MaeIII GTNAC 3 cut(s) 240, 484, 994
MalI GATC 1 cut(s) 611
MboI GATC 1 cut(s) 609
MboII GAAGA 2 cut(s) 409, 574
MfeI CAATTG 1 cut(s) 672
MhlI GDGCHC 7 cut(s) 15, 295, 367, 394, 454, 622, 1078
MluCI AATT 8 cut(s) 309, 332, 347, 513, 672, 726, 741, 932
MluI ACGCGT 1 cut(s) 538
MlyI GAGTC 2 cut(s) 413, 430
MnlI CCTC 8 cut(s) 25, 30, 228, 324, 392, 418, 460, 666
Mph1103I ATGCAT 2 cut(s) 763, 781
MroNI GCCGGC 2 cut(s) 33, 917
MroXI GAANNNNTTC 1 cut(s) 726
MseI TTAA 2 cut(s) 75, 764
MslI CAYNNNNRTG 1 cut(s) 884
MspI CCGG 7 cut(s) 9, 34, 91, 482, 918, 1023, 1079
MspR9I CCNGG 4 cut(s) 10, 91, 92, 482
MunI CAATTG 1 cut(s) 672
Mva1269I GAATGC 2 cut(s) 491, 779
MvnI CGCG 1 cut(s) 540
MwoI GCNNNNNNNGC 6 cut(s) 67, 143, 176, 626, 632, 927
NaeI GCCGGC 2 cut(s) 35, 919
NciI CCSGG 4 cut(s) 10, 91, 92, 482
NcoI CCATGG 1 cut(s) 630
NdeII GATC 1 cut(s) 609
NgoMIV GCCGGC 2 cut(s) 33, 917
NlaIII CATG 4 cut(s) 131, 634, 683, 711
NlaIV GGNNCC 8 cut(s) 13, 125, 364, 365, 453, 556, 715, 1056
NmeAIII GCCGAG 1 cut(s) 65
NmuCI GTSAC 1 cut(s) 994
NsiI ATGCAT 2 cut(s) 763, 781
NspI RCATGY 1 cut(s) 683
PaeR7I CTCGAG 1 cut(s) 387
PciI ACATGT 1 cut(s) 679
PcsI WCGNNNNNNNCGW 1 cut(s) 808
PctI GAATGC 2 cut(s) 491, 779
PdiI GCCGGC 2 cut(s) 35, 919
PdmI GAANNNNTTC 1 cut(s) 726
PfeI GAWTC 2 cut(s) 198, 853
PkrI GCNGC 9 cut(s) 52, 136, 139, 169, 230, 596, 628, 952, 1021
PleI GAGTC 2 cut(s) 413, 430
PpsI GAGTC 2 cut(s) 413, 430
Ppu21I YACGTR 1 cut(s) 1086
PscI ACATGT 1 cut(s) 679
PshBI ATTAAT 1 cut(s) 764
PspN4I GGNNCC 8 cut(s) 13, 125, 364, 365, 453, 556, 715, 1056
PspOMI GGGCCC 2 cut(s) 11, 363
PspPI GGNCC 8 cut(s) 11, 12, 88, 124, 363, 364, 634, 1055
PstI CTGCAG 1 cut(s) 596
RsaI GTAC 1 cut(s) 697
RsaNI GTAC 1 cut(s) 696
RseI CAYNNNNRTG 1 cut(s) 884
SaqAI TTAA 2 cut(s) 75, 764
SatI GCNGC 9 cut(s) 51, 135, 138, 168, 229, 595, 627, 951, 1020
Sau3AI GATC 1 cut(s) 609
Sau96I GGNCC 8 cut(s) 11, 12, 88, 124, 363, 364, 634, 1055
SchI GAGTC 2 cut(s) 413, 430
ScrFI CCNGG 4 cut(s) 10, 91, 92, 482
SduI GDGCHC 7 cut(s) 15, 295, 367, 394, 454, 622, 1078
SfaNI GCATC 2 cut(s) 188, 766
SfcI CTRYAG 1 cut(s) 592
SfiI GGCCNNNNNGGCC 1 cut(s) 632
Sfr274I CTCGAG 1 cut(s) 387
SinI GGWCC 2 cut(s) 124, 1055
SlaI CTCGAG 1 cut(s) 387
SmaI CCCGGG 1 cut(s) 92
SmiMI CAYNNNNRTG 1 cut(s) 884
SmlI CTYRAG 1 cut(s) 387
SmoI CTYRAG 1 cut(s) 387
Sse9I AATT 8 cut(s) 309, 332, 347, 513, 672, 726, 741, 932
SsiI CCGC 9 cut(s) 27, 31, 144, 202, 360, 627, 827, 951, 1019
SspI AATATT 1 cut(s) 532
SspMI CTAG 4 cut(s) 224, 659, 977, 999
StyD4I CCNGG 4 cut(s) 8, 89, 90, 480
StyI CCWWGG 1 cut(s) 630
TaiI ACGT 2 cut(s) 280, 1088
TaqI TCGA 1 cut(s) 388
TaqII GACCGA 1 cut(s) 1072
TasI AATT 8 cut(s) 309, 332, 347, 513, 672, 726, 741, 932
TauI GCSGC 3 cut(s) 629, 953, 1022
TfiI GAWTC 2 cut(s) 198, 853
Tru1I TTAA 2 cut(s) 75, 764
Tru9I TTAA 2 cut(s) 75, 764
TscAI CASTG 2 cut(s) 627, 990
TseFI GTSAC 1 cut(s) 994
TseI GCWGC 6 cut(s) 50, 134, 137, 167, 228, 594
Tsp45I GTSAC 1 cut(s) 994
TspGWI ACGGA 1 cut(s) 1061
TspMI CCCGGG 1 cut(s) 90
TspRI CASTG 2 cut(s) 627, 990
VneI GTGCAC 1 cut(s) 1074
VpaK11BI GGWCC 2 cut(s) 124, 1055
VspI ATTAAT 1 cut(s) 764
XagI CCTNNNNNAGG 1 cut(s) 222
XapI RAATTY 6 cut(s) 309, 332, 347, 513, 726, 932
XceI RCATGY 1 cut(s) 683
XhoI CTCGAG 1 cut(s) 387
XmaI CCCGGG 1 cut(s) 90
XmnI GAANNNNTTC 1 cut(s) 726
XspI CTAG 4 cut(s) 224, 659, 977, 999
Zsp2I ATGCAT 2 cut(s) 763, 781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.