RLG00000030293

Proton-conducting membrane transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0010702
Physical Location & Seq
Forward (+)
59074949 .. 59075560
612 bp
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UTR
Exon/CDS
Intron
RLM00000030293

Sequence Viewer

Length: 612 bp
ATGGCCCAAACGAAAGTAAAAAGACCTCTAGCTCATAGTTCAATTGGACATGTAGGTTATATTCGTACAGGTTTATCATGTGGAACCATAGAAGGAATTCAATCACTACTAATTGGTCTCTTTATTTATGCATTAATGACGATAGATGCATTCGCCATAGTTTTAGCATTACGGCAAACCCGTGTCAAATGTATAGCGGATTTGGGCGCTTTAGCCAAAACGAATCCTATTTCGGCTATTACCTTCGCCATTACTTTGATCATGGCCCCTTCCCAACAGTTGTACCTTGTCTTTGGGCGTCTTTGGCTTTACGAGAGAAAGCCCGCTGCAGAAAAAAGTCTTTCTATTTCCGTCCCGGATCATATTCCGGTACTTCCACAAAAGAGGAAATCGCAATGCATCTTTCTCAGCAGGCGTAGCTTGGAGTGGGAGTGTAGCGTGGGTAAGGTAAGGAAGCCGCCAAAGAGGCCTGTTGGAACAGCTGGAGTAAGAAGTCCCCAAGTGAGCGTATTATGGGGGACCGTCAAGCACATAGGGCAAGAATCAAAGGGGATCCCGTTGTTGCATAGCTCAGGTCGGAGCAAGGCGCCATCGGACTGTGGAAGAGCCTAA

Protein Analysis

204

Amino Acids

22.09

Weight (kDa)

10.3

Isoelectric Point (pI)

54.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proton_antipo_M PF00361 1 - 87 1.5e-18 NADH:quinone oxidoreductase/Mrp antiporter, TM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 586
AciI CCGC 3 cut(s) 197, 324, 458
AclWI GGATC 3 cut(s) 366, 547, 560
AcsI RAATTY 1 cut(s) 96
AcyI GRCGYC 2 cut(s) 298, 587
AfaI GTAC 3 cut(s) 67, 284, 372
AflIII ACRYGT 1 cut(s) 49
AgsI TTSAA 2 cut(s) 42, 101
AluBI AGCT 4 cut(s) 32, 420, 482, 570
AluI AGCT 4 cut(s) 32, 420, 482, 570
Alw26I GTCTC 1 cut(s) 122
AlwI GGATC 3 cut(s) 366, 547, 560
AoxI GGCC 3 cut(s) 3, 264, 467
ApeKI GCWGC 1 cut(s) 326
ApoI RAATTY 1 cut(s) 96
AseI ATTAAT 1 cut(s) 134
Asp700I GAANNNNTTC 1 cut(s) 96
AspLEI GCGC 2 cut(s) 209, 589
AspS9I GGNCC 3 cut(s) 4, 265, 519
AsuC2I CCSGG 1 cut(s) 356
AvaII GGWCC 1 cut(s) 519
BaeI ACNNNNGTAYC 2 cut(s) 266, 299
BamHI GGATCC 1 cut(s) 552
BanI GGYRCC 1 cut(s) 586
BbvI GCAGC 1 cut(s) 313
BccI CCATC 1 cut(s) 598
BceAI ACGGC 1 cut(s) 188
BclI TGATCA 1 cut(s) 258
BcnI CCSGG 1 cut(s) 356
BcoDI GTCTC 1 cut(s) 122
BfaI CTAG 1 cut(s) 29
BfmI CTRYAG 1 cut(s) 327
BfoI RGCGCY 2 cut(s) 210, 590
BglI GCCNNNNNGGC 1 cut(s) 466
BisI GCNGC 2 cut(s) 327, 458
BlsI GCNGC 2 cut(s) 328, 459
Bme1390I CCNGG 1 cut(s) 356
Bme18I GGWCC 1 cut(s) 519
BmgT120I GGNCC 3 cut(s) 4, 265, 519
BmiI GGNNCC 5 cut(s) 85, 267, 520, 554, 588
BmrFI CCNGG 1 cut(s) 356
BmsI GCATC 2 cut(s) 136, 408
BpmI CTGGAG 1 cut(s) 504
Bpu10I CCTNAGC 1 cut(s) 571
BpuMI CCSGG 1 cut(s) 356
BsaHI GRCGYC 2 cut(s) 298, 587
BsaI GGTCTC 1 cut(s) 122
BsaWI WCCGGW 1 cut(s) 367
BsaXI ACNNNNNCTCC 2 cut(s) 416, 446
Bse3DI GCAATG 1 cut(s) 401
BseMI GCAATG 1 cut(s) 401
BseMII CTCAG 2 cut(s) 421, 585
BseXI GCAGC 1 cut(s) 313
BshFI GGCC 3 cut(s) 5, 266, 469
BshNI GGYRCC 1 cut(s) 586
BsiSI CCGG 2 cut(s) 356, 368
BslFI GGGAC 3 cut(s) 338, 480, 532
BsmAI GTCTC 1 cut(s) 122
BsmFI GGGAC 3 cut(s) 338, 480, 532
BsmI GAATGC 1 cut(s) 149
BsnI GGCC 3 cut(s) 5, 266, 469
Bso31I GGTCTC 1 cut(s) 122
Bsp143I GATC 3 cut(s) 258, 358, 552
BspACI CCGC 3 cut(s) 197, 324, 458
BspANI GGCC 3 cut(s) 5, 266, 469
BspCNI CTCAG 2 cut(s) 420, 584
BspLI GGNNCC 5 cut(s) 85, 267, 520, 554, 588
BspMAI CTGCAG 1 cut(s) 331
BspPI GGATC 3 cut(s) 366, 547, 560
BspQI GCTCTTC 1 cut(s) 598
BspT107I GGYRCC 1 cut(s) 586
BspTNI GGTCTC 1 cut(s) 122
BsrDI GCAATG 1 cut(s) 401
BssMI GATC 3 cut(s) 258, 358, 552
BssNI GRCGYC 2 cut(s) 298, 587
Bst4CI ACNGT 3 cut(s) 279, 523, 599
Bst6I CTCTTC 1 cut(s) 598
BstACI GRCGYC 2 cut(s) 298, 587
BstC8I GCNNGC 2 cut(s) 324, 413
BstDEI CTNAG 2 cut(s) 407, 571
BstH2I RGCGCY 2 cut(s) 210, 590
BstHHI GCGC 2 cut(s) 209, 589
BstKTI GATC 3 cut(s) 261, 361, 555
BstMAI GTCTC 1 cut(s) 122
BstMBI GATC 3 cut(s) 258, 358, 552
BstMWI GCNNNNNNNGC 4 cut(s) 304, 417, 466, 535
BstNSI RCATGY 1 cut(s) 53
BstSCI CCNGG 1 cut(s) 354
BstSFI CTRYAG 1 cut(s) 327
BstV1I GCAGC 1 cut(s) 313
BstX2I RGATCY 1 cut(s) 552
BstYI RGATCY 1 cut(s) 552
BsuRI GGCC 3 cut(s) 5, 266, 469
Cac8I GCNNGC 2 cut(s) 324, 413
CfoI GCGC 2 cut(s) 209, 589
Cfr13I GGNCC 3 cut(s) 4, 265, 519
CseI GACGC 1 cut(s) 287
Csp6I GTAC 3 cut(s) 66, 283, 371
CviAII CATG 3 cut(s) 50, 78, 262
CviQI GTAC 3 cut(s) 66, 283, 371
DdeI CTNAG 2 cut(s) 407, 571
DinI GGCGCC 1 cut(s) 588
DpnI GATC 3 cut(s) 260, 360, 554
DpnII GATC 3 cut(s) 258, 358, 552
Eam1104I CTCTTC 1 cut(s) 598
EarI CTCTTC 1 cut(s) 598
Eco147I AGGCCT 1 cut(s) 469
Eco31I GGTCTC 1 cut(s) 122
Eco47I GGWCC 1 cut(s) 519
EcoRI GAATTC 1 cut(s) 96
EcoT22I ATGCAT 3 cut(s) 133, 151, 401
EgeI GGCGCC 1 cut(s) 588
EheI GGCGCC 1 cut(s) 588
FaeI CATG 3 cut(s) 53, 81, 265
FaqI GGGAC 3 cut(s) 338, 480, 532
FatI CATG 3 cut(s) 49, 77, 261
FauI CCCGC 1 cut(s) 331
FbaI TGATCA 1 cut(s) 258
Fnu4HI GCNGC 2 cut(s) 327, 458
Fsp4HI GCNGC 2 cut(s) 327, 458
FspBI CTAG 1 cut(s) 29
GlaI GCGC 2 cut(s) 208, 588
GluI GCNGC 2 cut(s) 327, 458
GsuI CTGGAG 1 cut(s) 504
HaeII RGCGCY 2 cut(s) 210, 590
HaeIII GGCC 3 cut(s) 5, 266, 469
HapII CCGG 2 cut(s) 356, 368
HgaI GACGC 1 cut(s) 287
HhaI GCGC 2 cut(s) 209, 589
Hin1I GRCGYC 2 cut(s) 298, 587
Hin1II CATG 3 cut(s) 53, 81, 265
Hin6I GCGC 2 cut(s) 207, 587
HinP1I GCGC 2 cut(s) 207, 587
HinfI GANTC 2 cut(s) 223, 542
HpaII CCGG 2 cut(s) 356, 368
Hpy188I TCNGA 2 cut(s) 579, 595
HpyAV CCTTC 3 cut(s) 86, 253, 279
HpyCH4III ACNGT 3 cut(s) 279, 523, 599
HpyCH4V TGCA 5 cut(s) 131, 149, 329, 399, 565
HpyF10VI GCNNNNNNNGC 4 cut(s) 304, 417, 466, 535
HpyF3I CTNAG 2 cut(s) 407, 571
Hsp92I GRCGYC 2 cut(s) 298, 587
Hsp92II CATG 3 cut(s) 53, 81, 265
HspAI GCGC 2 cut(s) 207, 587
KasI GGCGCC 1 cut(s) 586
Ksp22I TGATCA 1 cut(s) 258
Kzo9I GATC 3 cut(s) 258, 358, 552
LguI GCTCTTC 1 cut(s) 598
LmnI GCTCC 1 cut(s) 579
LpnPI CCDG 7 cut(s) 54, 369, 381, 397, 468, 483, 558
Lsp1109I GCAGC 1 cut(s) 313
LweI GCATC 2 cut(s) 136, 408
MaeI CTAG 1 cut(s) 29
MalI GATC 3 cut(s) 260, 360, 554
MboI GATC 3 cut(s) 258, 358, 552
MfeI CAATTG 1 cut(s) 42
MflI RGATCY 1 cut(s) 552
MluCI AATT 3 cut(s) 42, 96, 111
Mly113I GGCGCC 1 cut(s) 587
MmeI TCCRAC 2 cut(s) 454, 557
MnlI CCTC 3 cut(s) 36, 378, 459
Mph1103I ATGCAT 3 cut(s) 133, 151, 401
MroXI GAANNNNTTC 1 cut(s) 96
MseI TTAA 1 cut(s) 134
MspA1I CMGCKG 2 cut(s) 326, 482
MspI CCGG 2 cut(s) 356, 368
MspR9I CCNGG 1 cut(s) 356
MunI CAATTG 1 cut(s) 42
Mva1269I GAATGC 1 cut(s) 149
MwoI GCNNNNNNNGC 4 cut(s) 304, 417, 466, 535
NarI GGCGCC 1 cut(s) 587
NciI CCSGG 1 cut(s) 356
NdeII GATC 3 cut(s) 258, 358, 552
NlaIII CATG 3 cut(s) 53, 81, 265
NlaIV GGNNCC 5 cut(s) 85, 267, 520, 554, 588
NsiI ATGCAT 3 cut(s) 133, 151, 401
NspI RCATGY 1 cut(s) 53
PceI AGGCCT 1 cut(s) 469
PciI ACATGT 1 cut(s) 49
PciSI GCTCTTC 1 cut(s) 598
PcsI WCGNNNNNNNCGW 1 cut(s) 178
PctI GAATGC 1 cut(s) 149
PdmI GAANNNNTTC 1 cut(s) 96
PfeI GAWTC 2 cut(s) 223, 542
PfoI TCCNGGA 1 cut(s) 354
PkrI GCNGC 2 cut(s) 328, 459
PluTI GGCGCC 1 cut(s) 590
PscI ACATGT 1 cut(s) 49
PshBI ATTAAT 1 cut(s) 134
PspN4I GGNNCC 5 cut(s) 85, 267, 520, 554, 588
PspPI GGNCC 3 cut(s) 4, 265, 519
PstI CTGCAG 1 cut(s) 331
PsuI RGATCY 1 cut(s) 552
PvuII CAGCTG 1 cut(s) 482
RsaI GTAC 3 cut(s) 67, 284, 372
RsaNI GTAC 3 cut(s) 66, 283, 371
SapI GCTCTTC 1 cut(s) 598
SaqAI TTAA 1 cut(s) 134
SatI GCNGC 2 cut(s) 327, 458
Sau3AI GATC 3 cut(s) 258, 358, 552
Sau96I GGNCC 3 cut(s) 4, 265, 519
ScrFI CCNGG 1 cut(s) 356
SfaNI GCATC 2 cut(s) 136, 408
SfcI CTRYAG 1 cut(s) 327
SfoI GGCGCC 1 cut(s) 588
SinI GGWCC 1 cut(s) 519
Sse9I AATT 3 cut(s) 42, 96, 111
SseBI AGGCCT 1 cut(s) 469
SsiI CCGC 3 cut(s) 197, 324, 458
SspDI GGCGCC 1 cut(s) 586
SspMI CTAG 1 cut(s) 29
StuI AGGCCT 1 cut(s) 469
StyD4I CCNGG 1 cut(s) 354
TaaI ACNGT 3 cut(s) 279, 523, 599
TasI AATT 3 cut(s) 42, 96, 111
TauI GCSGC 1 cut(s) 460
TfiI GAWTC 2 cut(s) 223, 542
Tru1I TTAA 1 cut(s) 134
Tru9I TTAA 1 cut(s) 134
TseI GCWGC 1 cut(s) 326
TspGWI ACGGA 1 cut(s) 340
VpaK11BI GGWCC 1 cut(s) 519
VspI ATTAAT 1 cut(s) 134
XapI RAATTY 1 cut(s) 96
XceI RCATGY 1 cut(s) 53
XmnI GAANNNNTTC 1 cut(s) 96
XspI CTAG 1 cut(s) 29
Zsp2I ATGCAT 3 cut(s) 133, 151, 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.