RLG00000000025

Proton-conducting membrane transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Unknown
OG0010702
Physical Location & Seq
Forward (+)
141307 .. 144476
3170 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000025

Sequence Viewer

Length: 1179 bp
ATGTGGGCACCTGATATCTATGAGGGTTCACCCACCCCGGTTACAGCATTCCTTTCTATTGCGCCTAAAATTTCAATTTCTGCTAATATTTCACGCGTTTCTATTTATGGTTCCTATGGAGCTACATTGCAACAAATCTTCTTTTTCTGCAGCATTGCTTCTATGATCTTAGGAGCACTGGCCGCCATGGCCCAAACGAAAGTAAAAAGACCTCTAGCTCATAGTTCAATTGGACATGTAGGTTATATTCGTACAGGGTTATCATGTGGAACCATAGAAGGAATTCAATCACTACTAATTGGTCTCTTTATTTATGCATTAATGACGATAGATGCATTCGCCATAGTTTTAGCATTACGGCAAACCCGTGTCAAATATATAGCGGATTTGGGCGCTTTAGCCAAAACGAATCCTATTTCGGCTATTACCTTCGCCATTACTATGTTCTCATACGCAGGAATACCCCCGTTAGCCGGCTTTTGTAGCAAATTCTATTTGTTCTTCGCCGCTTTGGGTTGTGGGGCTTACTTCCTAGCCCCAGTGGGAGTAGTGACTAGCGTTATAGGTCGTTGGGCGGCCGGAAGGTTGCCACGAGTAAGTAAGTTTGGGGGACCGAAGTTGACCTTAGGTGTAATAGGGACTCCCAGTTACTGTGCGCGATCGTATACTGGGGTGCTCCCCGCCGGTTGTTGGAACGACGCGAGCCGGGCCGGGCCTCGATTCAGAAAGATGAAGGGCCAAAAAAACGGCGAAGTCCATCCGAACCGTCCAATGAAGAATAAAGAGGAGAGCAAAGCGCCAATGGCACGCGAAGCGCATGCGGAACGGGCACGGAGAAAAAAAAGTGTGGAGGAGAAGCAGCCGAGCTCATTCCCTTCGCTTCCTGGGCCCAAAGCACCGGGAAGGGGCCTAGAAGTGCCTACTACTACACCATACTACACTTGGCTCTACACATTTACAGAGCTAACCCCTGTCCGCGGAGCTAAGGGGGCTTCAATCCTTATTCCTTATCCCCCCGGGCCTTACTTATTCAGGGGAGAGAGTAGAGCCTCGAGAAGCACTGTTGAGAGGAAGGGCAGTTACACTGCTCCTCTTCATTCTCTACAGGGTTCCAACCCTTTCTTCAACATAGGTGACAACGAGCGGGGCAGAGATGGAAGAGATCGAACACGGGAATAA

Protein Analysis

393

Amino Acids

42.42

Weight (kDa)

10.03

Isoelectric Point (pI)

39.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proton_antipo_M PF00361 1 - 189 2.3e-53 NADH:quinone oxidoreductase/Mrp antiporter, TM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 7
AccBSI CCGCTC 1 cut(s) 1144
AccI GTMKAC 1 cut(s) 665
AccII CGCG 5 cut(s) 96, 658, 701, 810, 978
AciI CCGC 9 cut(s) 183, 383, 507, 575, 681, 821, 976, 978, 1144
AcoI YGGCCR 2 cut(s) 180, 576
AcsI RAATTY 3 cut(s) 69, 282, 488
AfaI GTAC 1 cut(s) 253
AfiI CCNNNNNNNGG 4 cut(s) 473, 690, 905, 977
AflIII ACRYGT 2 cut(s) 94, 235
AgsI TTSAA 5 cut(s) 75, 228, 287, 996, 1126
AjnI CCWGG 1 cut(s) 883
AjuI GAANNNNNNNTTGG 2 cut(s) 1106, 1138
AluBI AGCT 5 cut(s) 122, 218, 867, 964, 983
AluI AGCT 5 cut(s) 122, 218, 867, 964, 983
Alw21I GWGCWC 3 cut(s) 178, 678, 869
Alw26I GTCTC 1 cut(s) 308
AlwNI CAGNNNCTG 1 cut(s) 651
Ama87I CYCGRG 2 cut(s) 1016, 1051
AoxI GGCC 9 cut(s) 180, 189, 576, 708, 713, 736, 887, 907, 1019
ApaI GGGCCC 1 cut(s) 891
ApeKI GCWGC 2 cut(s) 150, 859
ApoI RAATTY 3 cut(s) 69, 282, 488
AseI ATTAAT 1 cut(s) 320
Asp700I GAANNNNTTC 1 cut(s) 282
AspLEI GCGC 5 cut(s) 64, 395, 658, 799, 817
AspS9I GGNCC 9 cut(s) 190, 611, 708, 713, 736, 887, 888, 907, 1019
AsuC2I CCSGG 6 cut(s) 38, 707, 712, 900, 1017, 1018
AsuHPI GGTGA 2 cut(s) 21, 1145
AvaI CYCGRG 2 cut(s) 1016, 1051
AvaII GGWCC 1 cut(s) 611
AxyI CCTNAGG 1 cut(s) 625
BaeGI GKGCMC 3 cut(s) 10, 832, 891
BanI GGYRCC 1 cut(s) 7
BanII GRGCYC 2 cut(s) 869, 891
BarI GAAGNNNNNNTAC 2 cut(s) 1064, 1096
BauI CACGAG 1 cut(s) 591
Bbv12I GWGCWC 3 cut(s) 178, 678, 869
BbvI GCAGC 2 cut(s) 162, 871
BccI CCATC 2 cut(s) 765, 1148
BceAI ACGGC 2 cut(s) 374, 763
BcgI CGANNNNNNTGC 2 cut(s) 800, 834
BciT130I CCWGG 1 cut(s) 885
BcnI CCSGG 6 cut(s) 38, 707, 712, 900, 1017, 1018
BcoDI GTCTC 1 cut(s) 308
BfaI CTAG 4 cut(s) 215, 533, 555, 911
BfmI CTRYAG 2 cut(s) 148, 1103
BfoI RGCGCY 2 cut(s) 396, 800
BglI GCCNNNNNGGC 1 cut(s) 188
BisI GCNGC 5 cut(s) 151, 183, 507, 576, 860
BlsI GCNGC 5 cut(s) 152, 184, 508, 577, 861
Bme1390I CCNGG 7 cut(s) 38, 707, 712, 885, 900, 1017, 1018
Bme18I GGWCC 1 cut(s) 611
BmeT110I CYCGRG 2 cut(s) 1016, 1051
BmgT120I GGNCC 9 cut(s) 190, 611, 708, 713, 736, 887, 888, 907, 1019
BmiI GGNNCC 7 cut(s) 9, 112, 271, 612, 889, 908, 1111
BmrFI CCNGG 7 cut(s) 38, 707, 712, 885, 900, 1017, 1018
BmrI ACTGGG 3 cut(s) 533, 639, 678
BmsI GCATC 1 cut(s) 322
BmuI ACTGGG 3 cut(s) 533, 639, 678
Bpu10I CCTNAGC 1 cut(s) 984
BpuMI CCSGG 6 cut(s) 38, 707, 712, 900, 1017, 1018
BsaI GGTCTC 1 cut(s) 308
BsaJI CCNNGG 6 cut(s) 36, 186, 884, 976, 1015, 1016
Bsc4I CCNNNNNNNGG 4 cut(s) 473, 690, 905, 977
Bse118I RCCGGY 2 cut(s) 473, 683
Bse1I ACTGG 4 cut(s) 183, 539, 645, 673
Bse21I CCTNAGG 1 cut(s) 625
Bse3DI GCAATG 2 cut(s) 125, 153
BseBI CCWGG 1 cut(s) 885
BseDI CCNNGG 6 cut(s) 36, 186, 884, 976, 1015, 1016
BseGI GGATG 1 cut(s) 757
BseLI CCNNNNNNNGG 4 cut(s) 473, 690, 905, 977
BseMI GCAATG 2 cut(s) 125, 153
BseNI ACTGG 4 cut(s) 183, 539, 645, 673
BseRI GAGGAG 3 cut(s) 800, 866, 1080
BseSI GKGCMC 3 cut(s) 10, 832, 891
BseX3I CGGCCG 1 cut(s) 576
BseXI GCAGC 2 cut(s) 162, 871
Bsh1236I CGCG 5 cut(s) 96, 658, 701, 810, 978
Bsh1285I CGRYCG 2 cut(s) 579, 662
BshFI GGCC 9 cut(s) 182, 191, 578, 710, 715, 738, 889, 909, 1021
BshNI GGYRCC 1 cut(s) 7
BsiEI CGRYCG 2 cut(s) 579, 662
BsiHKAI GWGCWC 3 cut(s) 178, 678, 869
BsiHKCI CYCGRG 2 cut(s) 1016, 1051
BsiSI CCGG 8 cut(s) 38, 474, 579, 684, 706, 711, 899, 1017
BslFI GGGAC 2 cut(s) 624, 652
BslI CCNNNNNNNGG 4 cut(s) 473, 690, 905, 977
BsmAI GTCTC 1 cut(s) 308
BsmFI GGGAC 2 cut(s) 624, 652
BsmI GAATGC 2 cut(s) 47, 335
BsnI GGCC 9 cut(s) 182, 191, 578, 710, 715, 738, 889, 909, 1021
Bso31I GGTCTC 1 cut(s) 308
BsoBI CYCGRG 2 cut(s) 1016, 1051
Bsp120I GGGCCC 1 cut(s) 887
Bsp1286I GDGCHC 6 cut(s) 10, 178, 678, 832, 869, 891
Bsp143I GATC 3 cut(s) 165, 659, 1162
Bsp19I CCATGG 1 cut(s) 186
BspACI CCGC 9 cut(s) 183, 383, 507, 575, 681, 821, 976, 978, 1144
BspANI GGCC 9 cut(s) 182, 191, 578, 710, 715, 738, 889, 909, 1021
BspFNI CGCG 5 cut(s) 96, 658, 701, 810, 978
BspLI GGNNCC 7 cut(s) 9, 112, 271, 612, 889, 908, 1111
BspMAI CTGCAG 1 cut(s) 152
BspT107I GGYRCC 1 cut(s) 7
BspTNI GGTCTC 1 cut(s) 308
BsrBI CCGCTC 1 cut(s) 1144
BsrDI GCAATG 2 cut(s) 125, 153
BsrFI RCCGGY 2 cut(s) 473, 683
BsrI ACTGG 4 cut(s) 183, 539, 645, 673
BssAI RCCGGY 2 cut(s) 473, 683
BssECI CCNNGG 6 cut(s) 36, 186, 884, 976, 1015, 1016
BssMI GATC 3 cut(s) 165, 659, 1162
BssNAI GTATAC 1 cut(s) 666
BssSI CACGAG 1 cut(s) 591
BssT1I CCWWGG 1 cut(s) 186
Bst1107I GTATAC 1 cut(s) 666
Bst2BI CACGAG 1 cut(s) 591
Bst2UI CCWGG 1 cut(s) 885
Bst4CI ACNGT 3 cut(s) 653, 767, 1063
Bst6I CTCTTC 2 cut(s) 1098, 1153
BstC8I GCNNGC 4 cut(s) 475, 703, 808, 819
BstDEI CTNAG 3 cut(s) 169, 625, 984
BstDSI CCRYGG 2 cut(s) 186, 976
BstF5I GGATG 1 cut(s) 757
BstFNI CGCG 5 cut(s) 96, 658, 701, 810, 978
BstH2I RGCGCY 2 cut(s) 396, 800
BstHHI GCGC 5 cut(s) 64, 395, 658, 799, 817
BstKTI GATC 3 cut(s) 168, 662, 1165
BstMAI GTCTC 1 cut(s) 308
BstMBI GATC 3 cut(s) 165, 659, 1162
BstMCI CGRYCG 2 cut(s) 579, 662
BstMWI GCNNNNNNNGC 9 cut(s) 182, 188, 483, 707, 803, 812, 827, 886, 989
BstNI CCWGG 1 cut(s) 885
BstNSI RCATGY 2 cut(s) 239, 821
BstSCI CCNGG 7 cut(s) 36, 705, 710, 883, 898, 1015, 1016
BstSFI CTRYAG 2 cut(s) 148, 1103
BstSLI GKGCMC 3 cut(s) 10, 832, 891
BstUI CGCG 5 cut(s) 96, 658, 701, 810, 978
BstV1I GCAGC 2 cut(s) 162, 871
BstZ17I GTATAC 1 cut(s) 666
BstZI CGGCCG 1 cut(s) 576
Bsu36I CCTNAGG 1 cut(s) 625
BsuRI GGCC 9 cut(s) 182, 191, 578, 710, 715, 738, 889, 909, 1021
BtgI CCRYGG 2 cut(s) 186, 976
BtsCI GGATG 1 cut(s) 757
BtsI GCAGTG 1 cut(s) 1083
BtsIMutI CAGTG 4 cut(s) 176, 546, 1059, 1083
Cac8I GCNNGC 4 cut(s) 475, 703, 808, 819
CaiI CAGNNNCTG 1 cut(s) 651
CfoI GCGC 5 cut(s) 64, 395, 658, 799, 817
Cfr10I RCCGGY 2 cut(s) 473, 683
Cfr13I GGNCC 9 cut(s) 190, 611, 708, 713, 736, 887, 888, 907, 1019
Cfr42I CCGCGG 1 cut(s) 979
Cfr9I CCCGGG 1 cut(s) 1016
CseI GACGC 1 cut(s) 707
Csp6I GTAC 1 cut(s) 252
CviAII CATG 4 cut(s) 187, 236, 264, 818
CviQI GTAC 1 cut(s) 252
DdeI CTNAG 3 cut(s) 169, 625, 984
DpnI GATC 3 cut(s) 167, 661, 1164
DpnII GATC 3 cut(s) 165, 659, 1162
EaeI YGGCCR 2 cut(s) 180, 576
EagI CGGCCG 1 cut(s) 576
Eam1104I CTCTTC 2 cut(s) 1098, 1153
EarI CTCTTC 2 cut(s) 1098, 1153
Ecl136II GAGCTC 1 cut(s) 867
EclXI CGGCCG 1 cut(s) 576
Eco130I CCWWGG 1 cut(s) 186
Eco24I GRGCYC 2 cut(s) 869, 891
Eco31I GGTCTC 1 cut(s) 308
Eco32I GATATC 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 611
Eco52I CGGCCG 1 cut(s) 576
Eco53kI GAGCTC 1 cut(s) 867
Eco81I CCTNAGG 1 cut(s) 625
Eco88I CYCGRG 2 cut(s) 1016, 1051
EcoICRI GAGCTC 1 cut(s) 867
EcoO109I RGGNCCY 1 cut(s) 907
EcoRI GAATTC 1 cut(s) 282
EcoRII CCWGG 1 cut(s) 883
EcoRV GATATC 1 cut(s) 16
EcoT14I CCWWGG 1 cut(s) 186
EcoT22I ATGCAT 2 cut(s) 319, 337
EcoT38I GRGCYC 2 cut(s) 869, 891
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 4 cut(s) 190, 239, 267, 821
FalI AAGNNNNNCTT 2 cut(s) 608, 640
FaqI GGGAC 2 cut(s) 624, 652
FatI CATG 4 cut(s) 186, 235, 263, 817
FauI CCCGC 2 cut(s) 688, 1137
FblI GTMKAC 1 cut(s) 665
Fnu4HI GCNGC 5 cut(s) 151, 183, 507, 576, 860
FokI GGATG 1 cut(s) 744
FriOI GRGCYC 2 cut(s) 869, 891
Fsp4HI GCNGC 5 cut(s) 151, 183, 507, 576, 860
FspBI CTAG 4 cut(s) 215, 533, 555, 911
GlaI GCGC 5 cut(s) 63, 394, 657, 798, 816
GluI GCNGC 5 cut(s) 151, 183, 507, 576, 860
HaeII RGCGCY 2 cut(s) 396, 800
HaeIII GGCC 9 cut(s) 182, 191, 578, 710, 715, 738, 889, 909, 1021
HapII CCGG 8 cut(s) 38, 474, 579, 684, 706, 711, 899, 1017
HgaI GACGC 1 cut(s) 707
HhaI GCGC 5 cut(s) 64, 395, 658, 799, 817
Hin1II CATG 4 cut(s) 190, 239, 267, 821
Hin6I GCGC 5 cut(s) 62, 393, 656, 797, 815
HinP1I GCGC 5 cut(s) 62, 393, 656, 797, 815
HincII GTYRAC 1 cut(s) 621
HindII GTYRAC 1 cut(s) 621
HinfI GANTC 3 cut(s) 409, 640, 720
HpaII CCGG 8 cut(s) 38, 474, 579, 684, 706, 711, 899, 1017
HphI GGTGA 2 cut(s) 21, 1145
Hpy166II GTNNAC 3 cut(s) 29, 621, 666
Hpy188I TCNGA 2 cut(s) 725, 762
Hpy188III TCNNGA 1 cut(s) 1053
Hpy8I GTNNAC 3 cut(s) 29, 621, 666
Hpy99I CGWCG 1 cut(s) 701
HpyAV CCTTC 7 cut(s) 272, 439, 576, 727, 885, 897, 1066
HpyCH4III ACNGT 3 cut(s) 653, 767, 1063
HpyCH4V TGCA 4 cut(s) 130, 150, 317, 335
HpyF10VI GCNNNNNNNGC 9 cut(s) 182, 188, 483, 707, 803, 812, 827, 886, 989
HpyF3I CTNAG 3 cut(s) 169, 625, 984
Hsp92II CATG 4 cut(s) 190, 239, 267, 821
HspAI GCGC 5 cut(s) 62, 393, 656, 797, 815
KroI GCCGGC 1 cut(s) 473
KroNI GCCGGC 1 cut(s) 475
KspI CCGCGG 1 cut(s) 979
Kzo9I GATC 3 cut(s) 165, 659, 1162
LmnI GCTCC 5 cut(s) 119, 173, 681, 980, 1093
Lsp1109I GCAGC 2 cut(s) 162, 871
LweI GCATC 1 cut(s) 322
MaeI CTAG 4 cut(s) 215, 533, 555, 911
MaeIII GTNAC 5 cut(s) 40, 550, 647, 1079, 1133
MalI GATC 3 cut(s) 167, 661, 1164
MbiI CCGCTC 1 cut(s) 1144
MboI GATC 3 cut(s) 165, 659, 1162
MboII GAAGA 6 cut(s) 130, 493, 787, 1085, 1114, 1170
MfeI CAATTG 1 cut(s) 228
MhlI GDGCHC 6 cut(s) 10, 178, 678, 832, 869, 891
MluCI AATT 6 cut(s) 69, 75, 228, 282, 297, 488
MluI ACGCGT 1 cut(s) 94
MlyI GAGTC 1 cut(s) 634
MmeI TCCRAC 2 cut(s) 671, 1137
MnlI CCTC 8 cut(s) 16, 222, 726, 778, 844, 1060, 1062, 1101
Mph1103I ATGCAT 2 cut(s) 319, 337
MroNI GCCGGC 1 cut(s) 473
MroXI GAANNNNTTC 1 cut(s) 282
MseI TTAA 1 cut(s) 320
MslI CAYNNNNRTG 1 cut(s) 440
MspA1I CMGCKG 1 cut(s) 978
MspI CCGG 8 cut(s) 38, 474, 579, 684, 706, 711, 899, 1017
MspR9I CCNGG 7 cut(s) 38, 707, 712, 885, 900, 1017, 1018
MunI CAATTG 1 cut(s) 228
Mva1269I GAATGC 2 cut(s) 47, 335
MvaI CCWGG 1 cut(s) 885
MvnI CGCG 5 cut(s) 96, 658, 701, 810, 978
MwoI GCNNNNNNNGC 9 cut(s) 182, 188, 483, 707, 803, 812, 827, 886, 989
NaeI GCCGGC 1 cut(s) 475
NciI CCSGG 6 cut(s) 38, 707, 712, 900, 1017, 1018
NcoI CCATGG 1 cut(s) 186
NdeII GATC 3 cut(s) 165, 659, 1162
NgoMIV GCCGGC 1 cut(s) 473
NlaIII CATG 4 cut(s) 190, 239, 267, 821
NlaIV GGNNCC 7 cut(s) 9, 112, 271, 612, 889, 908, 1111
NmeAIII GCCGAG 1 cut(s) 888
NmuCI GTSAC 2 cut(s) 550, 1133
NsiI ATGCAT 2 cut(s) 319, 337
NspI RCATGY 2 cut(s) 239, 821
PaeI GCATGC 1 cut(s) 821
PaeR7I CTCGAG 1 cut(s) 1051
PciI ACATGT 1 cut(s) 235
PcsI WCGNNNNNNNCGW 1 cut(s) 364
PctI GAATGC 2 cut(s) 47, 335
PdiI GCCGGC 1 cut(s) 475
PdmI GAANNNNTTC 1 cut(s) 282
PfeI GAWTC 2 cut(s) 409, 720
PkrI GCNGC 5 cut(s) 152, 184, 508, 577, 861
Ple19I CGATCG 1 cut(s) 662
PleI GAGTC 1 cut(s) 634
PpsI GAGTC 1 cut(s) 634
PscI ACATGT 1 cut(s) 235
PshBI ATTAAT 1 cut(s) 320
Psp124BI GAGCTC 1 cut(s) 869
Psp6I CCWGG 1 cut(s) 883
PspGI CCWGG 1 cut(s) 883
PspN4I GGNNCC 7 cut(s) 9, 112, 271, 612, 889, 908, 1111
PspOMI GGGCCC 1 cut(s) 887
PspPI GGNCC 9 cut(s) 190, 611, 708, 713, 736, 887, 888, 907, 1019
PstI CTGCAG 1 cut(s) 152
PstNI CAGNNNCTG 1 cut(s) 651
PvuI CGATCG 1 cut(s) 662
RsaI GTAC 1 cut(s) 253
RsaNI GTAC 1 cut(s) 252
RseI CAYNNNNRTG 1 cut(s) 440
SacI GAGCTC 1 cut(s) 869
SacII CCGCGG 1 cut(s) 979
SaqAI TTAA 1 cut(s) 320
SatI GCNGC 5 cut(s) 151, 183, 507, 576, 860
Sau3AI GATC 3 cut(s) 165, 659, 1162
Sau96I GGNCC 9 cut(s) 190, 611, 708, 713, 736, 887, 888, 907, 1019
SchI GAGTC 1 cut(s) 634
ScrFI CCNGG 7 cut(s) 38, 707, 712, 885, 900, 1017, 1018
SduI GDGCHC 6 cut(s) 10, 178, 678, 832, 869, 891
SfaNI GCATC 1 cut(s) 322
SfcI CTRYAG 2 cut(s) 148, 1103
SfiI GGCCNNNNNGGCC 1 cut(s) 188
Sfr274I CTCGAG 1 cut(s) 1051
Sfr303I CCGCGG 1 cut(s) 979
SgrBI CCGCGG 1 cut(s) 979
SinI GGWCC 1 cut(s) 611
SlaI CTCGAG 1 cut(s) 1051
SmaI CCCGGG 1 cut(s) 1018
SmiMI CAYNNNNRTG 1 cut(s) 440
SmlI CTYRAG 1 cut(s) 1051
SmoI CTYRAG 1 cut(s) 1051
SphI GCATGC 1 cut(s) 821
Sse9I AATT 6 cut(s) 69, 75, 228, 282, 297, 488
SsiI CCGC 9 cut(s) 183, 383, 507, 575, 681, 821, 976, 978, 1144
SspI AATATT 1 cut(s) 88
SspMI CTAG 4 cut(s) 215, 533, 555, 911
SstI GAGCTC 1 cut(s) 869
StyD4I CCNGG 7 cut(s) 36, 705, 710, 883, 898, 1015, 1016
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 3 cut(s) 653, 767, 1063
TaqI TCGA 3 cut(s) 718, 1052, 1165
TaqII GACCGA 1 cut(s) 628
TasI AATT 6 cut(s) 69, 75, 228, 282, 297, 488
TauI GCSGC 3 cut(s) 185, 509, 578
TfiI GAWTC 2 cut(s) 409, 720
Tru1I TTAA 1 cut(s) 320
Tru9I TTAA 1 cut(s) 320
TscAI CASTG 4 cut(s) 183, 546, 1066, 1090
TseFI GTSAC 2 cut(s) 550, 1133
TseI GCWGC 2 cut(s) 150, 859
Tsp45I GTSAC 2 cut(s) 550, 1133
TspDTI ATGAA 3 cut(s) 746, 788, 1085
TspGWI ACGGA 1 cut(s) 847
TspMI CCCGGG 1 cut(s) 1016
TspRI CASTG 4 cut(s) 183, 546, 1066, 1090
VpaK11BI GGWCC 1 cut(s) 611
VspI ATTAAT 1 cut(s) 320
XapI RAATTY 3 cut(s) 69, 282, 488
XceI RCATGY 2 cut(s) 239, 821
XcmI CCANNNNNNNNNTGG 1 cut(s) 939
XhoI CTCGAG 1 cut(s) 1051
XmaI CCCGGG 1 cut(s) 1016
XmiI GTMKAC 1 cut(s) 665
XmnI GAANNNNTTC 1 cut(s) 282
XspI CTAG 4 cut(s) 215, 533, 555, 911
Zsp2I ATGCAT 2 cut(s) 319, 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.