pycom520g00400

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_520
OG0017378
Physical Location & Seq
Forward (+)
342824 .. 348004
5181 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4563 bp
ATGCTCGCCGGGTGCTTCCACAGAAAAATCTCAAGGTGTTTGAGAGGTTTTGCGCAGGGCAATTGTATGCTGATTGTGAAGGTCCTTTTCCGTTGCATGATTTCTTGTATTTATAGTGCCCCATTCCTTGAAACCAATTCTGATTTGGATTGGGAGTCCTTGTCCCATTTCGACTCTAACTCGAACAAACCTACTCCCACTGGGATTCTGAATTTTCCTCTTTTCGGGACTCCATTCCTTGCCGGTCGAGCATCCTGGTTGCACCAGGACTTCCCCGACCCTTTTCTATTCACCCGGACTACTTGGGATAAGATTTGGCTGACCCTGCCAGCTTGCCCGGGAGTTATTATTCGGCCCATAGTATTTATCATCACCTTGGGCCGAGCACATCCTGCCGCTCGGCCGAACAATAATATTTTGGGCCCAAACATTGCCCCCTCGCTTCTGAGGTCGCCGACTGTTAACTCTTGGTCGGGCCTCGACCTTGAAGAAGCGAAAACGTTTATTTCCGGCGCATTTATGAGACATGATTGCACTTTCCTCGTTGCCCCCAACGCGTGGCCACGTGTCGATTCTACGCCGTTCTCAAAAGCTTTCAATCATGGTAAAACTGACGCCGCACGCAATCGTGCGTCCTGGATTTTCGACTCCTCGATCCCCTCTTTGCAGGTTCCCCAGATATTTGAAATGATTGGGAACTTTTCCCGGTTAAATTTTCCCCCAATTTCGAATTCCAGTCGTTTGATATTCATTCTGAAAAAAACTCCAAAATCTCCAATTCCTCATCCTTGTTCGCTCAAATCAAACCTTCCAGTTCTTCCTTCTGCAATTATTTTCTCCAGTTCTTCCGAAGTCTCTCCTACCATCTCTATAATGGCCCCTTTTATCTCGAAGCTCTCTGACGAGATCAACAAATGCAAGGATTTCATCGATCGGAACGATCGATCAAAACACTACGCTTCGAGAATGACATGGCCACCACTCATCAGATTCTGGGCCCCTTTTCAGGGATGCAGTGCCGTCGTGCATCACCAATCTCCTCAAGGAGCAGTGCCTAACACCCCTGCTTCAAGGGTTCGACTGGTCCAAATGGTGCTTGGCTCGGCCTCAAGGAGCTTGGCCCTCGACCACCACAGCCTGGGCCGCTTGGCCCTTGGTATCTATGATGCCATCCTGCTCTCGACCATGGAGATTACCATGGACAAGGAGCTGCTTATGGCGGCCCTGACTCTATGGTCTTCAGCCACCAACACCATGGTCCTTCCATTCGGCCCTCTCGGTCCTACAATCCTCGACATCTCAGCCATTCTTGGGACTTCTCCATCCGCCCTCCCAATCGACGCCGCTCTTTCTGGATGCCCCTCCCTCCTCGACCTGAAGGCACTTTTCGACGAGCGGGCCGTCGAAACGCTCAGCCGAGGCAATCGGGAGCCTTCAAAAGAAGAAGTTCAGAAGCTGCATAAAAACTTCTTCAACTACAATACCCTGATTCTCCACTTTGCCGGCCGAGGCAAGGAGAATCTCAGGAAAGGAGAACATGAAGCCTTCCTCTTCTATTGGTACAACAAATTTATTTGTTGTACCAGGTCGAACAAGTGCTTGGTCGAGAATATGCCGGTGGCCCAGGCCCTGGCTAGTGGTCATTCTCTGGCGCTCAGCCCGGCTATTCTCGCCAACCTCTTTCGTTGCTTGGCCGAGACGACCATCAACAAGATTGACCCGCACCAGAACGGACCCCTCTGGGTTTTCCAGCTCTGGCTGCAGGTCTATTTCTCTACTCTTCGGCCTGAGGTCCCTGCTTTCCAACGCTCGGCCGCCCTCGGCCCCCAACTGGCCTCTCGACCGACTCCTCATCACCGGGCTAACGAGGTCCTTAAGCACTTTTTCAATCTCGACGTTCTTTCGGACGACGAATTCCTGGTGTGCCGACGCCAGGAGTACCCTGCTTCGATTGGGCTTCCGACGGCAGCTTGGGCCGAGGCCGAGGAGGCAGGTCTCCGTCAGTCCTGGGGATCGATCGTACTGGCCCGCGACCTTCCCCTTGGTTGCGATGCTCGCCGAGCCAGTTGGGAGGTCTACCAGCCCCACTTCGTCGCCTGGCAGCTCGGCTACCTCCAAGGTTGCCCCATACCCCTGATCGTCTCTCGTTCCCTCCTGAGCCGGGGACGTCTTTATGGTTCTTCCGAGAGGGAGTGCCGGACGACGGAACAAGAGTTTCAGGATCGGTGCAAGAAGTTCCGTCTTCGGCCCATCGTTCCAGAATCCTTGGGCACCGACACTTTTGGGGACTGGTGGGACGAATACACCGGCGACTTCTTCGGCGCCTCGGTCGAAGACGTGGTGAGCAAAGTGTTTGGTGACCGGCCCAGACCAGCGCCCTCGACCCAACCTAAGGAGTCGAAAAAGAAGCTTGTCCTCATTACTAATTACTTTACTATTTCAGGGGGTCGCGGGTCGAAAACGGTCGAGGTGGTCGCCGCGGTGGCAAGGGCGAAGAAGACCCTTGTCTCCGAGCGGTCGGTCCCTGCCAAACGCCCCCATCGAGAGGCCGAGCCGGCGGAAGAAACCCCTCGCCCTTCTAAACGCGTCAAGAAATTGGCGAAGAAGGGGGACCGGGAAATTCATGTCGTCCCCAGTGACACTACTGGGACGCCGGCTCATGCCGGCTCTCCATCTGTGCCGGCTGCCCCGGCATCTCCAAACTCGAATCCTCCGGCCGTCTCCCAGGCCGATCCAATCGTCGCACCCGCTCCAGCTTCGGAGCCGGTCGTGGCGCCTGAAATAGGGAAGTCGGCTGCGCCTGCCGAAGCACCCCCGTCTCCAACGGTTTCTGGTCGTAATACTAATTGTTTTCTTTTTGTTCAGGATGTCGAGAGCGAGAGTGACGAAGTCCTGCTGGAACGCCGTCGTCGACCAGTCAACTCTCCTCCCGTCCATCCTCCTCCAGTCGTCGGGGCGACCGCTCGACCACGTCTTACTGCGGTGGATCGTGGCAAGAGACCTATTGCCGATCCTGAGGCCACGGCCGAAATGCCGATCCACCCGCAGGACGAAGACCCTCCCATTCCTCCACAGGAAGTCTCTTCGGCCTTTGTAAGTCTCGCTTTTTGTTGTGTTGATTTTTTTTTGTGTGTGGCACATGTTTTTAAAAGACTATTAAATGTCAGGTGCCCAAACATTCATTTCACCAGCAATTTTATGCGCCGAGGGGCGTTGTCTATATTTTCTGGCCGCCGTCGTGTTCCCTGGGTTCGAGCAATGACCCAGAGGGCATGGCCGAAGTCTCCTCTCGGCAGGTCTAAAACAATCCCCTTTTTTTCTTTATGCACTATATCACGACGTCTTACTTTCCCTCACAAAGTTTTTTCCTTTTATGTGCAGCCATCGTGGGAAGTGGAGCTCGACGCTCTTCTTCAGAGCACGACCGGTGAGGCCGGCTCTTCTGCTGCTCCCGCCGAACCAGCGGGCGCCGTGCCCGAGGCCGAAGTCTTCGTGAAGCTGCACGAAGTCCTGTCTCTTAATGCCTCCCAAGCTCTCCGATGCAAGGGCCTTGACTCTGTTGGAGAGTGTCTCAACGACCTTGCCAGCGGTGGTCACCTGAGCACGGAGAATATCTTTTTCCTCACCGACCTCCTGGAGCGCACTCGGCAACACTTCTACATCTTCGAGCGAGCCCTTCAAGCCGAGGATGACTTGAAGGCTGCCAAGGTCGCGCAAGAGGCCGGCCGAGCTGAGATGGAGGCAATCAAAGCTAAGAAGGCGAAGCTGACCGAGTTTGATCGCCAGATTTTTGACCTCCAGCGTCAAATTTCTGACCTTCAACGGCAAAGGTCTGCTGCTGCTGCCGAGCTTGAGAAGGACTTCGGGGCCAACAAGGCTCGGCTGACGGCCTTCGCTGCTGGTGCACGGCATGTCCAGCAGCTGCAGTACAACAGGAAGACCAGGCAGGCTGAGGTACATGAAGGAATTTTAACCCGGGGTGTTGAGTTTCATAATTTATCGAAAGACCAACTGAGAATCAATATTGTATGCAAGTATTTTCATCACATTTATATTTACGTTTTGCCTTTAATTACAATTTGTCCATTTAATTTAATCTCGTCAAATCAAACTCAATCCCCCCCCAATATTGTGTTAAGTCTTAGTCATTTGAATTGTTTTAAGTATTTTACGTTTTGTGTTTTTACCCCCGGTCCAGAACGATCCCTACTTACATCATTACTACAATTGTCAAAAAGAGGGTTTAATTTGTGTGCGTGTAAATCTCGCATCACTGCTGCCGAGCTTGAGAAGGACTTCGGGGCCAACAAGGCTCGGCTGACGGCCTTCGCTGCTGGTGCACGGCATGTCCAGCAGCTGCAGTACAACAGGAAGACCAGGCAGGCTGAGATCATCCTGGGCGAAGCGAAGTGGCTGGAGCTAAAAGCTTGTATTTGTATTTCTTGTAATGATTTTTTGTTATCAATACAATGGTCGTTTTGCTATCAATACAATGGTCGTCTTGCTATCAATACAATGGTCTTACTCTTGCATTTCCCATGTAATTGGATAGTACTTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans Pkinase cNMP_binding

Protein Analysis

1521

Amino Acids

169.03

Weight (kDa)

9.12

Isoelectric Point (pI)

53.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMD PF10536 386 - 765 4.3e-33 Plant mobile domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1234
AatII GACGTC 2 cut(s) 2170, 3343
Acc16I TGCGCA 1 cut(s) 54
Acc36I ACCTGC 4 cut(s) 658, 1753, 1982, 3285
AccB1I GGYRCC 5 cut(s) 2270, 2321, 2773, 3168, 3467
AccB7I CCANNNNNTGG 3 cut(s) 558, 1138, 1630
AccBSI CCGCTC 6 cut(s) 398, 1346, 1396, 2515, 2750, 2963
AccI GTMKAC 2 cut(s) 2076, 2911
AccII CGCG 6 cut(s) 557, 2031, 2451, 2480, 2586, 3713
AclI AACGTT 1 cut(s) 500
AclWI GGATC 8 cut(s) 649, 2020, 2229, 2726, 2994, 3005, 3031, 4197
AcsI RAATTY 8 cut(s) 211, 712, 730, 1568, 1913, 2619, 3807, 3966
AcuI CTGAAG 3 cut(s) 1224, 1397, 3398
AcvI CACGTG 1 cut(s) 566
AcyI GRCGYC 9 cut(s) 615, 1341, 1930, 2167, 2322, 2651, 2774, 3340, 3468
AfaI GTAC 8 cut(s) 1562, 1582, 1940, 2022, 3929, 3957, 4364, 4554
AflII CTTAAG 1 cut(s) 1874
AflIII ACRYGT 4 cut(s) 555, 565, 2584, 3139
AgeI ACCGGT 1 cut(s) 3425
AjiI CACGTC 2 cut(s) 2338, 2972
AjuI GAANNNNNNNTTGG 4 cut(s) 715, 747, 1583, 1615
Alw21I GWGCWC 6 cut(s) 388, 3402, 3422, 3605, 3907, 4342
Alw26I GTCTC 8 cut(s) 169, 208, 219, 219, 517, 693, 810, 859
Alw44I GTGCAC 2 cut(s) 3903, 4338
AlwI GGATC 8 cut(s) 649, 2020, 2229, 2726, 2994, 3005, 3031, 4197
AlwNI CAGNNNCTG 5 cut(s) 993, 1456, 1630, 3922, 4357
Ama87I CYCGRG 3 cut(s) 337, 3476, 3975
ApaI GGGCCC 2 cut(s) 425, 1000
ApaLI GTGCAC 2 cut(s) 3903, 4338
ApoI RAATTY 8 cut(s) 211, 712, 730, 1568, 1913, 2619, 3807, 3966
AsiGI ACCGGT 1 cut(s) 3425
Asp700I GAANNNNTTC 3 cut(s) 1446, 3860, 4295
AsuII TTCGAA 1 cut(s) 728
AvaI CYCGRG 3 cut(s) 337, 3476, 3975
AxyI CCTNAGG 3 cut(s) 1788, 2391, 3015
BaeGI GKGCMC 8 cut(s) 121, 425, 1000, 2273, 3173, 3477, 3907, 4342
BalI TGGCCA 2 cut(s) 562, 976
BanI GGYRCC 5 cut(s) 2270, 2321, 2773, 3168, 3467
BanII GRGCYC 4 cut(s) 425, 1000, 3402, 3676
BbrPI CACGTG 1 cut(s) 566
BbsI GAAGAC 8 cut(s) 1230, 2234, 2340, 2504, 3060, 3481, 3944, 4379
Bbv12I GWGCWC 6 cut(s) 388, 3402, 3422, 3605, 3907, 4342
BbvCI CCTCAGC 1 cut(s) 3951
BcgI CGANNNNNNTGC 4 cut(s) 523, 557, 1003, 1037
BfaI CTAG 1 cut(s) 1635
BfmI CTRYAG 3 cut(s) 1760, 3923, 4358
BfoI RGCGCY 5 cut(s) 1655, 2325, 2378, 2777, 3471
BfrI CTTAAG 1 cut(s) 1874
BfuAI ACCTGC 4 cut(s) 658, 1753, 1982, 3285
BglI GCCNNNNNGGC 5 cut(s) 1988, 2483, 2555, 3875, 4310
BlpI GCTNAGC 2 cut(s) 1412, 1655
BmcAI AGTACT 1 cut(s) 4554
BmeT110I CYCGRG 3 cut(s) 337, 3476, 3975
BmgBI CACGTC 2 cut(s) 2338, 2972
BmrI ACTGGG 3 cut(s) 210, 2628, 2655
BmsI GCATC 9 cut(s) 260, 1001, 1036, 1156, 1346, 2041, 2702, 3530, 4278
BmuI ACTGGG 3 cut(s) 210, 2628, 2655
BoxI GACNNNNGTC 1 cut(s) 2504
BpiI GAAGAC 8 cut(s) 1230, 2234, 2340, 2504, 3060, 3481, 3944, 4379
BplI GAGNNNNNCTC 4 cut(s) 3422, 3454, 3555, 3587
BpmI CTGGAG 6 cut(s) 823, 2736, 2928, 3656, 3782, 4436
Bpu10I CCTNAGC 3 cut(s) 2156, 3599, 3951
Bpu1102I GCTNAGC 2 cut(s) 1412, 1655
Bpu14I TTCGAA 1 cut(s) 728
BpuEI CTTGAG 5 cut(s) 16, 1026, 1093, 3872, 4307
Bsa29I ATCGAT 3 cut(s) 930, 943, 2015
BsaAI YACGTR 1 cut(s) 566
BsaHI GRCGYC 9 cut(s) 615, 1341, 1930, 2167, 2322, 2651, 2774, 3340, 3468
BsaI GGTCTC 2 cut(s) 2000, 2992
BsaWI WCCGGW 1 cut(s) 3425
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bse21I CCTNAGG 3 cut(s) 1788, 2391, 3015
Bse3DI GCAATG 2 cut(s) 429, 3264
BseCI ATCGAT 3 cut(s) 930, 943, 2015
BseMI GCAATG 2 cut(s) 429, 3264
BseRI GAGGAG 8 cut(s) 640, 1029, 1358, 1839, 2000, 2916, 2931, 3276
BseSI GKGCMC 8 cut(s) 121, 425, 1000, 2273, 3173, 3477, 3907, 4342
BseX3I CGGCCG 6 cut(s) 401, 1504, 1812, 2715, 3024, 3724
BsgI GTGCAG 2 cut(s) 3398, 3485
Bsh1236I CGCG 6 cut(s) 557, 2031, 2451, 2480, 2586, 3713
BshNI GGYRCC 5 cut(s) 2270, 2321, 2773, 3168, 3467
BshTI ACCGGT 1 cut(s) 3425
BshVI ATCGAT 3 cut(s) 930, 943, 2015
BsiHKAI GWGCWC 6 cut(s) 388, 3402, 3422, 3605, 3907, 4342
BsiHKCI CYCGRG 3 cut(s) 337, 3476, 3975
BsmBI CGTCTC 4 cut(s) 1691, 2146, 2725, 2823
Bso31I GGTCTC 2 cut(s) 2000, 2992
BsoBI CYCGRG 3 cut(s) 337, 3476, 3975
Bsp119I TTCGAA 1 cut(s) 728
Bsp120I GGGCCC 2 cut(s) 421, 996
Bsp1720I GCTNAGC 2 cut(s) 1412, 1655
Bsp19I CCATGG 3 cut(s) 1185, 1197, 1254
BspDI ATCGAT 3 cut(s) 930, 943, 2015
BspFNI CGCG 6 cut(s) 557, 2031, 2451, 2480, 2586, 3713
BspMAI CTGCAG 3 cut(s) 1764, 3927, 4362
BspMI ACCTGC 4 cut(s) 658, 1753, 1982, 3285
BspPI GGATC 8 cut(s) 649, 2020, 2229, 2726, 2994, 3005, 3031, 4197
BspQI GCTCTTC 2 cut(s) 3414, 3445
BspT104I TTCGAA 1 cut(s) 728
BspT107I GGYRCC 5 cut(s) 2270, 2321, 2773, 3168, 3467
BspTI CTTAAG 1 cut(s) 1874
BspTNI GGTCTC 2 cut(s) 2000, 2992
BsrBI CCGCTC 6 cut(s) 398, 1346, 1396, 2515, 2750, 2963
BsrDI GCAATG 2 cut(s) 429, 3264
BssNI GRCGYC 9 cut(s) 615, 1341, 1930, 2167, 2322, 2651, 2774, 3340, 3468
BssT1I CCWWGG 9 cut(s) 375, 1153, 1185, 1197, 1254, 2041, 2116, 2265, 3705
Bst4CI ACNGT 3 cut(s) 460, 2464, 2827
Bst6I CTCTTC 5 cut(s) 1556, 1785, 3088, 3414, 3445
BstACI GRCGYC 9 cut(s) 615, 1341, 1930, 2167, 2322, 2651, 2774, 3340, 3468
BstAFI CTTAAG 1 cut(s) 1874
BstAPI GCANNNNNTGC 1 cut(s) 392
BstBAI YACGTR 1 cut(s) 566
BstBI TTCGAA 1 cut(s) 728
BstDSI CCRYGG 5 cut(s) 1185, 1197, 1254, 2478, 3021
BstEII GGTNACC 2 cut(s) 2357, 3593
BstENI CCTNNNNNAGG 1 cut(s) 2781
BstFNI CGCG 6 cut(s) 557, 2031, 2451, 2480, 2586, 3713
BstH2I RGCGCY 5 cut(s) 1655, 2325, 2378, 2777, 3471
BstNSI RCATGY 3 cut(s) 3143, 3914, 4349
BstPAI GACNNNNGTC 1 cut(s) 2504
BstPI GGTNACC 2 cut(s) 2357, 3593
BstSFI CTRYAG 3 cut(s) 1760, 3923, 4358
BstSLI GKGCMC 8 cut(s) 121, 425, 1000, 2273, 3173, 3477, 3907, 4342
BstUI CGCG 6 cut(s) 557, 2031, 2451, 2480, 2586, 3713
BstV2I GAAGAC 8 cut(s) 1230, 2234, 2340, 2504, 3060, 3481, 3944, 4379
BstXI CCANNNNNNTGG 2 cut(s) 1255, 4545
BstZI CGGCCG 6 cut(s) 401, 1504, 1812, 2715, 3024, 3724
Bsu15I ATCGAT 3 cut(s) 930, 943, 2015
Bsu36I CCTNAGG 3 cut(s) 1788, 2391, 3015
BsuTUI ATCGAT 3 cut(s) 930, 943, 2015
BtgI CCRYGG 5 cut(s) 1185, 1197, 1254, 2478, 3021
BtgZI GCGATG 1 cut(s) 2064
BtrI CACGTC 2 cut(s) 2338, 2972
BtsI GCAGTG 3 cut(s) 1021, 1056, 4272
BtsIMutI CAGTG 5 cut(s) 198, 1021, 1056, 2641, 4272
BveI ACCTGC 4 cut(s) 658, 1753, 1982, 3285
CaiI CAGNNNCTG 5 cut(s) 993, 1456, 1630, 3922, 4357
Cfr42I CCGCGG 1 cut(s) 2481
Cfr9I CCCGGG 2 cut(s) 337, 3975
ClaI ATCGAT 3 cut(s) 930, 943, 2015
CseI GACGC 8 cut(s) 621, 623, 1349, 1938, 2575, 2659, 3413, 3791
CsiI ACCWGGT 1 cut(s) 1583
Csp6I GTAC 8 cut(s) 1561, 1581, 1939, 2021, 3928, 3956, 4363, 4553
CspAI ACCGGT 1 cut(s) 3425
CviQI GTAC 8 cut(s) 1561, 1581, 1939, 2021, 3928, 3956, 4363, 4553
DinI GGCGCC 3 cut(s) 2323, 2775, 3469
DraI TTTAAA 1 cut(s) 3148
DrdI GACNNNNNNGTC 1 cut(s) 1234
DseDI GACNNNNNNGTC 1 cut(s) 1234
EagI CGGCCG 6 cut(s) 401, 1504, 1812, 2715, 3024, 3724
Eam1104I CTCTTC 5 cut(s) 1556, 1785, 3088, 3414, 3445
EarI CTCTTC 5 cut(s) 1556, 1785, 3088, 3414, 3445
EciI GGCGGA 2 cut(s) 1315, 2573
Ecl136II GAGCTC 1 cut(s) 3400
EclXI CGGCCG 6 cut(s) 401, 1504, 1812, 2715, 3024, 3724
Eco130I CCWWGG 9 cut(s) 375, 1153, 1185, 1197, 1254, 2041, 2116, 2265, 3705
Eco24I GRGCYC 4 cut(s) 425, 1000, 3402, 3676
Eco31I GGTCTC 2 cut(s) 2000, 2992
Eco52I CGGCCG 6 cut(s) 401, 1504, 1812, 2715, 3024, 3724
Eco53kI GAGCTC 1 cut(s) 3400
Eco57I CTGAAG 3 cut(s) 1224, 1397, 3398
Eco72I CACGTG 1 cut(s) 566
Eco81I CCTNAGG 3 cut(s) 1788, 2391, 3015
Eco88I CYCGRG 3 cut(s) 337, 3476, 3975
Eco91I GGTNACC 2 cut(s) 2357, 3593
EcoICRI GAGCTC 1 cut(s) 3400
EcoNI CCTNNNNNAGG 1 cut(s) 2781
EcoO109I RGGNCCY 6 cut(s) 82, 997, 1627, 1792, 1870, 3547
EcoO65I GGTNACC 2 cut(s) 2357, 3593
EcoRI GAATTC 2 cut(s) 730, 1913
EcoT14I CCWWGG 9 cut(s) 375, 1153, 1185, 1197, 1254, 2041, 2116, 2265, 3705
EcoT38I GRGCYC 4 cut(s) 425, 1000, 3402, 3676
EgeI GGCGCC 3 cut(s) 2323, 2775, 3469
EheI GGCGCC 3 cut(s) 2323, 2775, 3469
ErhI CCWWGG 9 cut(s) 375, 1153, 1185, 1197, 1254, 2041, 2116, 2265, 3705
Esp3I CGTCTC 4 cut(s) 1691, 2146, 2725, 2823
FalI AAGNNNNNCTT 2 cut(s) 3088, 3120
FauI CCCGC 8 cut(s) 1389, 1728, 2036, 2444, 2755, 3051, 3457, 3460
FblI GTMKAC 2 cut(s) 2076, 2911
FriOI GRGCYC 4 cut(s) 425, 1000, 3402, 3676
FseI GGCCGGCC 1 cut(s) 3726
FspBI CTAG 1 cut(s) 1635
FspI TGCGCA 1 cut(s) 54
GsuI CTGGAG 6 cut(s) 823, 2736, 2928, 3656, 3782, 4436
HaeII RGCGCY 5 cut(s) 1655, 2325, 2378, 2777, 3471
HgaI GACGC 8 cut(s) 621, 623, 1349, 1938, 2575, 2659, 3413, 3791
Hin1I GRCGYC 9 cut(s) 615, 1341, 1930, 2167, 2322, 2651, 2774, 3340, 3468
HincII GTYRAC 3 cut(s) 463, 2912, 2920
HindII GTYRAC 3 cut(s) 463, 2912, 2920
HindIII AAGCTT 3 cut(s) 591, 2408, 4425
HpaI GTTAAC 1 cut(s) 463
Hpy166II GTNNAC 6 cut(s) 463, 2077, 2912, 2920, 3905, 4340
Hpy8I GTNNAC 6 cut(s) 463, 2077, 2912, 2920, 3905, 4340
HpyCH4III ACNGT 3 cut(s) 460, 2464, 2827
HpyCH4IV ACGT 9 cut(s) 500, 565, 1896, 2167, 2337, 2971, 3340, 4059, 4172
HpySE526I ACGT 9 cut(s) 500, 565, 1896, 2167, 2337, 2971, 3340, 4059, 4172
Hsp92I GRCGYC 9 cut(s) 615, 1341, 1930, 2167, 2322, 2651, 2774, 3340, 3468
KasI GGCGCC 3 cut(s) 2321, 2773, 3467
KroI GCCGGC 7 cut(s) 1502, 2554, 2653, 2663, 2680, 3434, 3722
KroNI GCCGGC 7 cut(s) 1504, 2556, 2655, 2665, 2682, 3436, 3724
KspAI GTTAAC 1 cut(s) 463
KspI CCGCGG 1 cut(s) 2481
LguI GCTCTTC 2 cut(s) 3414, 3445
LweI GCATC 9 cut(s) 260, 1001, 1036, 1156, 1346, 2041, 2702, 3530, 4278
MabI ACCWGGT 1 cut(s) 1583
MaeI CTAG 1 cut(s) 1635
MaeII ACGT 9 cut(s) 500, 565, 1896, 2167, 2337, 2971, 3340, 4059, 4172
MaeIII GTNAC 4 cut(s) 2357, 2636, 2882, 3593
MbiI CCGCTC 6 cut(s) 398, 1346, 1396, 2515, 2750, 2963
MfeI CAATTG 2 cut(s) 61, 4226
MlsI TGGCCA 2 cut(s) 562, 976
MluI ACGCGT 2 cut(s) 555, 2584
MluNI TGGCCA 2 cut(s) 562, 976
Mly113I GGCGCC 3 cut(s) 2322, 2774, 3468
MlyI GAGTC 8 cut(s) 164, 167, 223, 641, 1222, 1840, 2405, 3548
MmeI TCCRAC 4 cut(s) 1828, 1985, 2846, 3541
Mox20I TGGCCA 2 cut(s) 562, 976
MroNI GCCGGC 7 cut(s) 1502, 2554, 2653, 2663, 2680, 3434, 3722
MroXI GAANNNNTTC 3 cut(s) 1446, 3860, 4295
MscI TGGCCA 2 cut(s) 562, 976
Msp20I TGGCCA 2 cut(s) 562, 976
MspA1I CMGCKG 5 cut(s) 2480, 3464, 3588, 3922, 4357
MspCI CTTAAG 1 cut(s) 1874
MunI CAATTG 2 cut(s) 61, 4226
MvnI CGCG 6 cut(s) 557, 2031, 2451, 2480, 2586, 3713
NaeI GCCGGC 7 cut(s) 1504, 2556, 2655, 2665, 2682, 3436, 3724
NarI GGCGCC 3 cut(s) 2322, 2774, 3468
NcoI CCATGG 3 cut(s) 1185, 1197, 1254
NgoMIV GCCGGC 7 cut(s) 1502, 2554, 2653, 2663, 2680, 3434, 3722
NmuCI GTSAC 4 cut(s) 2357, 2636, 2882, 3593
NsbI TGCGCA 1 cut(s) 54
NspI RCATGY 3 cut(s) 3143, 3914, 4349
NspV TTCGAA 1 cut(s) 728
PasI CCCWGGG 1 cut(s) 3246
PciI ACATGT 1 cut(s) 3139
PciSI GCTCTTC 2 cut(s) 3414, 3445
PcsI WCGNNNNNNNCGW 5 cut(s) 936, 940, 1398, 2539, 2908
PdiI GCCGGC 7 cut(s) 1504, 2556, 2655, 2665, 2682, 3436, 3724
PdmI GAANNNNTTC 3 cut(s) 1446, 3860, 4295
PfeI GAWTC 8 cut(s) 205, 572, 990, 1489, 1519, 2261, 2707, 4017
PflFI GACNNNGTC 2 cut(s) 2888, 2970
PflMI CCANNNNNTGG 3 cut(s) 558, 1138, 1630
PfoI TCCNGGA 2 cut(s) 635, 3633
PinAI ACCGGT 1 cut(s) 3425
Ple19I CGATCG 3 cut(s) 934, 943, 2019
PleI GAGTC 8 cut(s) 163, 167, 223, 641, 1222, 1840, 2404, 3548
PluTI GGCGCC 3 cut(s) 2325, 2777, 3471
PmaCI CACGTG 1 cut(s) 566
PmlI CACGTG 1 cut(s) 566
PpsI GAGTC 8 cut(s) 163, 167, 223, 641, 1222, 1840, 2404, 3548
Ppu21I YACGTR 1 cut(s) 566
PpuMI RGGWCCY 3 cut(s) 82, 1792, 1870
PscI ACATGT 1 cut(s) 3139
PshAI GACNNNNGTC 1 cut(s) 2504
Psp124BI GAGCTC 1 cut(s) 3402
Psp1406I AACGTT 1 cut(s) 500
Psp5II RGGWCCY 3 cut(s) 82, 1792, 1870
PspCI CACGTG 1 cut(s) 566
PspEI GGTNACC 2 cut(s) 2357, 3593
PspOMI GGGCCC 2 cut(s) 421, 996
PspPPI RGGWCCY 3 cut(s) 82, 1792, 1870
PsrI GAACNNNNNNTAC 2 cut(s) 4191, 4223
PstI CTGCAG 3 cut(s) 1764, 3927, 4362
PstNI CAGNNNCTG 5 cut(s) 993, 1456, 1630, 3922, 4357
PsyI GACNNNGTC 2 cut(s) 2888, 2970
PvuI CGATCG 3 cut(s) 934, 943, 2019
PvuII CAGCTG 2 cut(s) 3922, 4357
RigI GGCCGGCC 1 cut(s) 3726
RsaI GTAC 8 cut(s) 1562, 1582, 1940, 2022, 3929, 3957, 4364, 4554
RsaNI GTAC 8 cut(s) 1561, 1581, 1939, 2021, 3928, 3956, 4363, 4553
SacI GAGCTC 1 cut(s) 3402
SacII CCGCGG 1 cut(s) 2481
SalI GTCGAC 1 cut(s) 2910
SapI GCTCTTC 2 cut(s) 3414, 3445
ScaI AGTACT 1 cut(s) 4554
SchI GAGTC 8 cut(s) 164, 167, 223, 641, 1222, 1840, 2405, 3548
SexAI ACCWGGT 1 cut(s) 1583
SfaNI GCATC 9 cut(s) 260, 1001, 1036, 1156, 1346, 2041, 2702, 3530, 4278
SfcI CTRYAG 3 cut(s) 1760, 3923, 4358
SfoI GGCGCC 3 cut(s) 2323, 2775, 3469
Sfr303I CCGCGG 1 cut(s) 2481
SfuI TTCGAA 1 cut(s) 728
SgrAI CRCCGGYG 1 cut(s) 2306
SgrBI CCGCGG 1 cut(s) 2481
SmaI CCCGGG 2 cut(s) 339, 3977
SmlI CTYRAG 6 cut(s) 31, 1041, 1108, 1874, 3851, 4286
SmoI CTYRAG 6 cut(s) 31, 1041, 1108, 1874, 3851, 4286
SspDI GGCGCC 3 cut(s) 2321, 2773, 3467
SspI AATATT 3 cut(s) 415, 4024, 4129
SspMI CTAG 1 cut(s) 1635
SstI GAGCTC 1 cut(s) 3402
StyI CCWWGG 9 cut(s) 375, 1153, 1185, 1197, 1254, 2041, 2116, 2265, 3705
TaaI ACNGT 3 cut(s) 460, 2464, 2827
TaiI ACGT 9 cut(s) 503, 568, 1899, 2170, 2340, 2974, 3343, 4062, 4175
TaqII GACCGA 5 cut(s) 1268, 1858, 2317, 2509, 3785
TatI WGTACW 3 cut(s) 3927, 4362, 4552
TauI GCSGC 8 cut(s) 398, 620, 1146, 1223, 1346, 1817, 2480, 3234
TfiI GAWTC 8 cut(s) 205, 572, 990, 1489, 1519, 2261, 2707, 4017
TscAI CASTG 5 cut(s) 205, 1021, 1056, 2641, 4279
TseFI GTSAC 4 cut(s) 2357, 2636, 2882, 3593
Tsp45I GTSAC 4 cut(s) 2357, 2636, 2882, 3593
TspDTI ATGAA 8 cut(s) 739, 916, 1554, 2612, 3170, 3975, 3980, 4031
TspGWI ACGGA 6 cut(s) 80, 1746, 1988, 2219, 2228, 3620
TspMI CCCGGG 2 cut(s) 337, 3975
TspRI CASTG 5 cut(s) 205, 1021, 1056, 2641, 4279
Tth111I GACNNNGTC 2 cut(s) 2888, 2970
Van91I CCANNNNNTGG 3 cut(s) 558, 1138, 1630
Vha464I CTTAAG 1 cut(s) 1874
VneI GTGCAC 2 cut(s) 3903, 4338
XagI CCTNNNNNAGG 1 cut(s) 2781
XapI RAATTY 8 cut(s) 211, 712, 730, 1568, 1913, 2619, 3807, 3966
XceI RCATGY 3 cut(s) 3143, 3914, 4349
XcmI CCANNNNNNNNNTGG 4 cut(s) 142, 871, 1094, 1252
XmaI CCCGGG 2 cut(s) 337, 3975
XmiI GTMKAC 2 cut(s) 2076, 2911
XmnI GAANNNNTTC 3 cut(s) 1446, 3860, 4295
XspI CTAG 1 cut(s) 1635
ZraI GACGTC 2 cut(s) 2168, 3341
ZrmI AGTACT 1 cut(s) 4554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.