RchiOBHm_Chr7g0207931

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0017447
Physical Location & Seq
Reverse (-)
25254644 .. 25258501
3858 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1290 bp
ATGGACCCGAGGCTAACAGAGGGGGCGATACTGAAGATCACAACGGAGTTTGTTGACGACAACTATAAGCCTATCCTGCAAGTGATCGACTTGAAGCAGATCCACACACAGCGCGGCGGCGGCCAGGTCACTGAGCGGTACAGGGTGGTCATCTCCGATGGGTCCCACACTGCGCAGGGGATACTGAGCACTCAGCAGAACTTCCTGGTTCAGCAAGGCCACCTTCAGAAGGGCTCCTTTGTCTGCCTAAATCAATTCATCTGCACCACCATCCAAGGCCGCATGATCATCATTATTGTCTTATTGGATGTTCTAGTTAATAAATGTGATATCATTGGACAGGCTGCCAGATTTTCGAATGCACTATCCTCACGGACAAGACTTGTAGATCAAACAACTGAGACAATTATAAGCGAACCCTACAGTCTTCCGGCGATGGACGCGAGGCTAACGGAGGGGGCGATACTGAAGATCACGACGGAGTATTTTGACGACAACTATAAGCCTATCCTACAAGTGATCGACTTGAAGCAAATCCACACACATGGCGGCGGCCAGGTCACTGAACGGTACAGGGTGGCCGTATCCGATGGGTCCCACACCGAGCAGGGGATGCTGGCCACTCAGCAGAACTTCCTGGTTCAGCAAGGCCACCTTCAGAAGGGCTCCGTTGTCCGCCTGAAGCAATTCACCTGCCCCACCATCCAAGGCCGCAAGATCATCCTTATTGTCGTATTGGATGTTCTAGTTGATAAATGTGATATCATTGGACAGGCTGCCAGATTTTCGAATGCACTATCCCCACGGACAAGACTTGTAGATCAAACAACTGAGACAATTATAAGCGAACCCTACAGTCTTCCGGCGATGGACGCGAGGCTAACGGAGGGGGCGATACTGAACATCACGACGGAGTATTTTGACGACAACTATAAGCCTATCCTGCAAGTGATCGACTTGAAGCAAATCCACACACATGGCGGCGGCCAGGTCACTGAACGGTACAGGGTGGCCGTATCCGATGGGTACCACACCGAGCAGGGGATGCTGGCCACTCAGCAGAACTTCCTGGTTCAGCAAGGCCACCTTCAGAAGGGCTCCGTTGTCCGCCTGAATCAATTCACCTGCACCACCATCCAAGGCCGCAAGATCATCATTATAATCTCATTGAATGTGCTAGTTGATAAATGTGATATCATTGGACAGGCTGCCGGATTTTCGAATGCACTATCCCAATGGACAATACTTATAGATCAAACAACCGAGCAATTCGGTTCCTTGGGGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon zf-CCHC

Protein Analysis

429

Amino Acids

47.68

Weight (kDa)

6.42

Isoelectric Point (pI)

30.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 5 - 106 9.1e-26 Replication factor-A protein 1, N-terminal domain
Rep-A_N PF04057 150 - 250 8.2e-28 Replication factor-A protein 1, N-terminal domain
Rep-A_N PF04057 294 - 394 1.1e-28 Replication factor-A protein 1, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 410, 842, 1160
AarI CACCTGC 2 cut(s) 701, 1133
Acc16I TGCGCA 1 cut(s) 174
Acc36I ACCTGC 2 cut(s) 701, 1133
Acc65I GGTACC 1 cut(s) 1026
AccB1I GGYRCC 1 cut(s) 1026
AccBSI CCGCTC 1 cut(s) 136
AccII CGCG 3 cut(s) 114, 443, 875
AclWI GGATC 1 cut(s) 94
AcoI YGGCCR 7 cut(s) 121, 553, 579, 618, 985, 1011, 1050
AcuI CTGAAG 6 cut(s) 53, 209, 488, 641, 701, 1073
AfaI GTAC 4 cut(s) 140, 572, 1004, 1028
AfiI CCNNNNNNNGG 5 cut(s) 229, 609, 661, 1041, 1093
AgsI TTSAA 4 cut(s) 94, 529, 961, 1171
AjnI CCWGG 6 cut(s) 123, 204, 555, 636, 987, 1068
Alw21I GWGCWC 1 cut(s) 191
Alw26I GTCTC 2 cut(s) 395, 827
AlwI GGATC 1 cut(s) 94
Ama87I CYCGRG 1 cut(s) 7
ApeKI GCWGC 3 cut(s) 344, 776, 1208
Asp700I GAANNNNTTC 2 cut(s) 686, 1118
Asp718I GGTACC 1 cut(s) 1026
AspLEI GCGC 2 cut(s) 114, 175
AspS9I GGNCC 3 cut(s) 4, 162, 594
AsuHPI GGTGA 2 cut(s) 682, 1114
AsuII TTCGAA 3 cut(s) 356, 788, 1220
AvaI CYCGRG 1 cut(s) 7
AvaII GGWCC 3 cut(s) 4, 162, 594
BalI TGGCCA 2 cut(s) 620, 1052
BanI GGYRCC 1 cut(s) 1026
BanII GRGCYC 3 cut(s) 236, 668, 1100
BbsI GAAGAC 2 cut(s) 419, 851
Bbv12I GWGCWC 1 cut(s) 191
BbvI GCAGC 3 cut(s) 331, 763, 1195
BccI CCATC 8 cut(s) 152, 278, 430, 584, 710, 862, 1016, 1142
BceAI ACGGC 2 cut(s) 566, 998
BciT130I CCWGG 6 cut(s) 125, 206, 557, 638, 989, 1070
BciVI GTATCC 3 cut(s) 174, 595, 1027
BclI TGATCA 1 cut(s) 285
BcoDI GTCTC 2 cut(s) 395, 827
BfaI CTAG 3 cut(s) 314, 746, 1178
BfmI CTRYAG 2 cut(s) 421, 853
BfuAI ACCTGC 2 cut(s) 701, 1133
BfuI GTATCC 3 cut(s) 174, 595, 1027
Bme1390I CCNGG 6 cut(s) 125, 206, 557, 638, 989, 1070
Bme18I GGWCC 3 cut(s) 4, 162, 594
BmeT110I CYCGRG 1 cut(s) 7
BmgT120I GGNCC 3 cut(s) 4, 162, 594
BmrFI CCNGG 6 cut(s) 125, 206, 557, 638, 989, 1070
BmsI GCATC 2 cut(s) 603, 1035
BpiI GAAGAC 2 cut(s) 419, 851
Bpu14I TTCGAA 3 cut(s) 356, 788, 1220
BsaJI CCNNGG 6 cut(s) 8, 274, 706, 803, 1138, 1278
Bsc4I CCNNNNNNNGG 5 cut(s) 229, 609, 661, 1041, 1093
BseBI CCWGG 6 cut(s) 125, 206, 557, 638, 989, 1070
BseDI CCNNGG 6 cut(s) 8, 274, 706, 803, 1138, 1278
BseGI GGATG 8 cut(s) 270, 313, 618, 702, 720, 745, 1050, 1134
BseLI CCNNNNNNNGG 5 cut(s) 229, 609, 661, 1041, 1093
BseMII CTCAG 7 cut(s) 123, 176, 206, 390, 638, 822, 1070
BseXI GCAGC 3 cut(s) 331, 763, 1195
BsgI GTGCAG 2 cut(s) 247, 1111
Bsh1236I CGCG 3 cut(s) 114, 443, 875
BshNI GGYRCC 1 cut(s) 1026
BsiHKAI GWGCWC 1 cut(s) 191
BsiHKCI CYCGRG 1 cut(s) 7
BsiSI CCGG 3 cut(s) 431, 863, 1212
BslFI GGGAC 2 cut(s) 148, 580
BslI CCNNNNNNNGG 5 cut(s) 229, 609, 661, 1041, 1093
BsmAI GTCTC 2 cut(s) 395, 827
BsmFI GGGAC 2 cut(s) 148, 580
BsmI GAATGC 3 cut(s) 364, 796, 1228
BsoBI CYCGRG 1 cut(s) 7
Bsp119I TTCGAA 3 cut(s) 356, 788, 1220
Bsp1286I GDGCHC 4 cut(s) 191, 236, 668, 1100
BspCNI CTCAG 7 cut(s) 124, 177, 205, 391, 637, 823, 1069
BspFNI CGCG 3 cut(s) 114, 443, 875
BspMI ACCTGC 2 cut(s) 701, 1133
BspPI GGATC 1 cut(s) 94
BspT104I TTCGAA 3 cut(s) 356, 788, 1220
BspT107I GGYRCC 1 cut(s) 1026
BsrBI CCGCTC 1 cut(s) 136
BssECI CCNNGG 6 cut(s) 8, 274, 706, 803, 1138, 1278
BssT1I CCWWGG 4 cut(s) 274, 706, 1138, 1278
Bst2UI CCWGG 6 cut(s) 125, 206, 557, 638, 989, 1070
Bst4CI ACNGT 4 cut(s) 425, 570, 857, 1002
BstAPI GCANNNNNTGC 2 cut(s) 613, 1045
BstBI TTCGAA 3 cut(s) 356, 788, 1220
BstC8I GCNNGC 2 cut(s) 618, 1050
BstDEI CTNAG 7 cut(s) 132, 185, 192, 399, 624, 831, 1056
BstDSI CCRYGG 1 cut(s) 803
BstENI CCTNNNNNAGG 3 cut(s) 227, 659, 1091
BstF5I GGATG 8 cut(s) 270, 313, 618, 702, 720, 745, 1050, 1134
BstFNI CGCG 3 cut(s) 114, 443, 875
BstHHI GCGC 2 cut(s) 114, 175
BstMAI GTCTC 2 cut(s) 395, 827
BstMWI GCNNNNNNNGC 7 cut(s) 76, 120, 440, 613, 872, 943, 1045
BstNI CCWGG 6 cut(s) 125, 206, 557, 638, 989, 1070
BstSCI CCNGG 6 cut(s) 123, 204, 555, 636, 987, 1068
BstSFI CTRYAG 2 cut(s) 421, 853
BstUI CGCG 3 cut(s) 114, 443, 875
BstV1I GCAGC 3 cut(s) 331, 763, 1195
BstV2I GAAGAC 2 cut(s) 419, 851
BstX2I RGATCY 1 cut(s) 99
BstXI CCANNNNNNTGG 2 cut(s) 545, 977
BstYI RGATCY 1 cut(s) 99
BsuI GTATCC 3 cut(s) 174, 595, 1027
BtgI CCRYGG 1 cut(s) 803
BtgZI GCGATG 2 cut(s) 449, 881
BtsCI GGATG 8 cut(s) 270, 313, 618, 702, 720, 745, 1050, 1134
BtsI GCAGTG 1 cut(s) 168
BtsIMutI CAGTG 4 cut(s) 129, 168, 561, 993
BveI ACCTGC 2 cut(s) 701, 1133
Cac8I GCNNGC 2 cut(s) 618, 1050
CfoI GCGC 2 cut(s) 114, 175
Cfr13I GGNCC 3 cut(s) 4, 162, 594
CseI GACGC 2 cut(s) 449, 881
Csp6I GTAC 4 cut(s) 139, 571, 1003, 1027
CviAII CATG 3 cut(s) 283, 545, 977
CviQI GTAC 4 cut(s) 139, 571, 1003, 1027
DdeI CTNAG 7 cut(s) 132, 185, 192, 399, 624, 831, 1056
EaeI YGGCCR 7 cut(s) 121, 553, 579, 618, 985, 1011, 1050
EciI GGCGGA 2 cut(s) 665, 1097
Eco130I CCWWGG 4 cut(s) 274, 706, 1138, 1278
Eco24I GRGCYC 3 cut(s) 236, 668, 1100
Eco32I GATATC 3 cut(s) 331, 763, 1195
Eco47I GGWCC 3 cut(s) 4, 162, 594
Eco57I CTGAAG 6 cut(s) 53, 209, 488, 641, 701, 1073
Eco88I CYCGRG 1 cut(s) 7
EcoNI CCTNNNNNAGG 3 cut(s) 227, 659, 1091
EcoO109I RGGNCCY 2 cut(s) 162, 594
EcoRII CCWGG 6 cut(s) 123, 204, 555, 636, 987, 1068
EcoRV GATATC 3 cut(s) 331, 763, 1195
EcoT14I CCWWGG 4 cut(s) 274, 706, 1138, 1278
EcoT38I GRGCYC 3 cut(s) 236, 668, 1100
ErhI CCWWGG 4 cut(s) 274, 706, 1138, 1278
FaeI CATG 3 cut(s) 286, 548, 980
FalI AAGNNNNNCTT 8 cut(s) 207, 239, 221, 253, 639, 671, 1071, 1103
FaqI GGGAC 2 cut(s) 148, 580
FatI CATG 3 cut(s) 282, 544, 976
FbaI TGATCA 1 cut(s) 285
FokI GGATG 8 cut(s) 257, 320, 625, 689, 707, 752, 1057, 1121
FriOI GRGCYC 3 cut(s) 236, 668, 1100
FspBI CTAG 3 cut(s) 314, 746, 1178
FspI TGCGCA 1 cut(s) 174
GlaI GCGC 2 cut(s) 113, 174
HapII CCGG 3 cut(s) 431, 863, 1212
HgaI GACGC 2 cut(s) 449, 881
HhaI GCGC 2 cut(s) 114, 175
Hin1II CATG 3 cut(s) 286, 548, 980
Hin6I GCGC 2 cut(s) 112, 173
HinP1I GCGC 2 cut(s) 112, 173
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HinfI GANTC 1 cut(s) 1114
HpaII CCGG 3 cut(s) 431, 863, 1212
HphI GGTGA 2 cut(s) 682, 1114
Hpy166II GTNNAC 1 cut(s) 55
Hpy188I TCNGA 6 cut(s) 157, 228, 589, 660, 1021, 1092
Hpy188III TCNNGA 2 cut(s) 475, 907
Hpy8I GTNNAC 1 cut(s) 55
Hpy99I CGWCG 2 cut(s) 481, 913
HpyAV CCTTC 6 cut(s) 223, 233, 655, 665, 1087, 1097
HpyCH4III ACNGT 4 cut(s) 425, 570, 857, 1002
HpyCH4V TGCA 7 cut(s) 79, 264, 362, 794, 946, 1128, 1226
HpyF10VI GCNNNNNNNGC 7 cut(s) 76, 120, 440, 613, 872, 943, 1045
HpyF3I CTNAG 7 cut(s) 132, 185, 192, 399, 624, 831, 1056
Hsp92II CATG 3 cut(s) 286, 548, 980
HspAI GCGC 2 cut(s) 112, 173
KflI GGGWCCC 2 cut(s) 162, 594
KpnI GGTACC 1 cut(s) 1030
Ksp22I TGATCA 1 cut(s) 285
LmnI GCTCC 3 cut(s) 239, 671, 1103
Lsp1109I GCAGC 3 cut(s) 331, 763, 1195
LweI GCATC 2 cut(s) 603, 1035
MaeI CTAG 3 cut(s) 314, 746, 1178
MaeIII GTNAC 3 cut(s) 127, 559, 991
MbiI CCGCTC 1 cut(s) 136
MboII GAAGA 4 cut(s) 46, 419, 481, 851
MflI RGATCY 1 cut(s) 99
MhlI GDGCHC 4 cut(s) 191, 236, 668, 1100
MlsI TGGCCA 2 cut(s) 620, 1052
MluCI AATT 6 cut(s) 254, 405, 686, 837, 1118, 1268
MluNI TGGCCA 2 cut(s) 620, 1052
MnlI CCTC 7 cut(s) 3, 13, 379, 438, 448, 870, 880
Mox20I TGGCCA 2 cut(s) 620, 1052
MroXI GAANNNNTTC 2 cut(s) 686, 1118
MscI TGGCCA 2 cut(s) 620, 1052
MseI TTAA 1 cut(s) 318
MslI CAYNNNNRTG 2 cut(s) 543, 975
Msp20I TGGCCA 2 cut(s) 620, 1052
MspI CCGG 3 cut(s) 431, 863, 1212
MspR9I CCNGG 6 cut(s) 125, 206, 557, 638, 989, 1070
Mva1269I GAATGC 3 cut(s) 364, 796, 1228
MvaI CCWGG 6 cut(s) 125, 206, 557, 638, 989, 1070
MvnI CGCG 3 cut(s) 114, 443, 875
MwoI GCNNNNNNNGC 7 cut(s) 76, 120, 440, 613, 872, 943, 1045
NlaIII CATG 3 cut(s) 286, 548, 980
NmuCI GTSAC 3 cut(s) 127, 559, 991
NsbI TGCGCA 1 cut(s) 174
NspV TTCGAA 3 cut(s) 356, 788, 1220
PaqCI CACCTGC 2 cut(s) 701, 1133
PcsI WCGNNNNNNNCGW 2 cut(s) 458, 890
PctI GAATGC 3 cut(s) 364, 796, 1228
PdmI GAANNNNTTC 2 cut(s) 686, 1118
PfeI GAWTC 1 cut(s) 1114
PpuMI RGGWCCY 2 cut(s) 162, 594
PsiI TTATAA 3 cut(s) 410, 842, 1160
Psp5II RGGWCCY 2 cut(s) 162, 594
Psp6I CCWGG 6 cut(s) 123, 204, 555, 636, 987, 1068
PspGI CCWGG 6 cut(s) 123, 204, 555, 636, 987, 1068
PspPI GGNCC 3 cut(s) 4, 162, 594
PspPPI RGGWCCY 2 cut(s) 162, 594
PsrI GAACNNNNNNTAC 2 cut(s) 726, 758
PsuI RGATCY 1 cut(s) 99
RsaI GTAC 4 cut(s) 140, 572, 1004, 1028
RsaNI GTAC 4 cut(s) 139, 571, 1003, 1027
RseI CAYNNNNRTG 2 cut(s) 543, 975
SaqAI TTAA 1 cut(s) 318
Sau96I GGNCC 3 cut(s) 4, 162, 594
ScrFI CCNGG 6 cut(s) 125, 206, 557, 638, 989, 1070
SduI GDGCHC 4 cut(s) 191, 236, 668, 1100
SetI ASST 8 cut(s) 129, 225, 561, 657, 695, 993, 1089, 1127
SfaNI GCATC 2 cut(s) 603, 1035
SfcI CTRYAG 2 cut(s) 421, 853
SfuI TTCGAA 3 cut(s) 356, 788, 1220
SinI GGWCC 3 cut(s) 4, 162, 594
SmiMI CAYNNNNRTG 2 cut(s) 543, 975
Sse9I AATT 6 cut(s) 254, 405, 686, 837, 1118, 1268
SspMI CTAG 3 cut(s) 314, 746, 1178
StyD4I CCNGG 6 cut(s) 123, 204, 555, 636, 987, 1068
StyI CCWWGG 4 cut(s) 274, 706, 1138, 1278
TaaI ACNGT 4 cut(s) 425, 570, 857, 1002
TaqI TCGA 6 cut(s) 87, 356, 522, 788, 954, 1220
TasI AATT 6 cut(s) 254, 405, 686, 837, 1118, 1268
TfiI GAWTC 1 cut(s) 1114
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TscAI CASTG 4 cut(s) 136, 175, 568, 1000
TseFI GTSAC 3 cut(s) 127, 559, 991
TseI GCWGC 3 cut(s) 344, 776, 1208
Tsp45I GTSAC 3 cut(s) 127, 559, 991
TspDTI ATGAA 1 cut(s) 247
TspGWI ACGGA 9 cut(s) 59, 388, 467, 494, 658, 820, 899, 926, 1090
TspRI CASTG 4 cut(s) 136, 175, 568, 1000
VpaK11BI GGWCC 3 cut(s) 4, 162, 594
XagI CCTNNNNNAGG 3 cut(s) 227, 659, 1091
XmnI GAANNNNTTC 2 cut(s) 686, 1118
XspI CTAG 3 cut(s) 314, 746, 1178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.