Rh7AG236000

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0017447
Physical Location & Seq
Forward (+)
23024837 .. 23040250
15414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG236000.1

Sequence Viewer

Length: 1296 bp
ATGGACGCGAGGCTAACGGAGGGGGCGATACTGAAGATCACGACGGACTTTGTTGACGACAACTATAAGCCTATCCTGCAAGTGATCGGCTTGAAGCAAATCCACACACACGGCGGCGGCTGGGGCGGCCAGGTCACTGAACGGTACAGGGTGACCGTATCCGATGGGTCCCACACCAAGCAGGGGATGCTGCTCGCTCACCAGAACTTCCTGGTTCAGCAAGGCCACCTTCAGAAGGGCTCCGTTGTCCGCCTGAATCAATTCACCTGCCCCACCATCCAAGGCCGCAAGATCATCATTATTGTCTTATTGGATGTTCTAGTTAATAAATGTGATATCATTGGACAGGCTGCCAGATTTTCGAATGCACTATCCTCACGGACAAGACTTGTAGATCAAACAACTGAGACAATTATAAGCGAACCCTACAGTCTTCCGGCGATGGACGCGAGGCTAACGGAGGGGGCGATACTGAAGATCACGACGGAGTATTTTGACGACAACTATAAGCCTATCCTACAAGTGATCGACTTGAAGCAAATCCACACACATGGCGGCGGCCAGGTCACTGAACGGTACAGGGTGGCCGTATCCGATGGGTCCCACACCGAGCAGGGGATGCTGGCCACTCAGCAGAACTTCCTGGTTCAGCAAGGCCACCTTCAGAAGGGCTCCGTTGTCCGCCTGAAGCAATTCACCTGCCCCACCATCCAAGGCCGCAAGATCATCCTTATTGTCGTATTGGATGTTCTAGTTGATAAATGTGATATCATTGGACAGGCTGCCAGATTTTCGAATGCACTATCCCCACGGACAAGACTTGTAGATCAAACAACTGAGACAATTATAAGCGAACCCTACAGTCTTCCGGCGATGGACGCGAGGCTAACGGAGGGGGCGATACTGAACATCACGACGGAGTATTTTGACGACAACTATAAGCCTATCCTGCAAGTGATCGACTTGAAGCAAATCCACACACATGGCGGCGGCCAGGTCACTGAACGGTACAGGGTGGCCGTATCCGATGGGTACCACACCGAGCAGGGGATGCTGGCCACTCAGCAGAACTTCCTGGTTCAGCAAGGCCACCTTCAGAAGGGCTCCGTTGTCCGCCTGAATCAATTCACCTGCACCACCATCCAAGGCCGCAAGATCATCATTATAATCTCATTGAATGTGCTAGTTGATAAATGTGATATCATTGGACAGGCTGCCGGATTTTCGAATGCACTATCCCAATGGACAATACTTATAGATCAAACAACCGAGCAATTCGGTTCCTTGGGGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon zf-CCHC

Protein Analysis

431

Amino Acids

47.79

Weight (kDa)

7.21

Isoelectric Point (pI)

29.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 5 - 108 2.8e-25 Replication factor-A protein 1, N-terminal domain
Rep-A_N PF04057 152 - 252 8.2e-28 Replication factor-A protein 1, N-terminal domain
Rep-A_N PF04057 296 - 396 1.1e-28 Replication factor-A protein 1, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 416, 848, 1166
AarI CACCTGC 3 cut(s) 275, 707, 1139
Acc36I ACCTGC 3 cut(s) 275, 707, 1139
Acc65I GGTACC 1 cut(s) 1032
AccB1I GGYRCC 1 cut(s) 1032
AccII CGCG 3 cut(s) 8, 449, 881
AcoI YGGCCR 7 cut(s) 127, 559, 585, 624, 991, 1017, 1056
AcuI CTGAAG 6 cut(s) 53, 215, 494, 647, 707, 1079
AfaI GTAC 4 cut(s) 146, 578, 1010, 1034
AfiI CCNNNNNNNGG 6 cut(s) 183, 235, 615, 667, 1047, 1099
AgsI TTSAA 4 cut(s) 94, 535, 967, 1177
AjnI CCWGG 6 cut(s) 129, 210, 561, 642, 993, 1074
Alw26I GTCTC 2 cut(s) 401, 833
ApeKI GCWGC 4 cut(s) 190, 350, 782, 1214
Asp700I GAANNNNTTC 3 cut(s) 260, 692, 1124
Asp718I GGTACC 1 cut(s) 1032
AspS9I GGNCC 2 cut(s) 168, 600
AsuHPI GGTGA 5 cut(s) 163, 191, 256, 688, 1120
AsuII TTCGAA 3 cut(s) 362, 794, 1226
AvaII GGWCC 2 cut(s) 168, 600
BalI TGGCCA 2 cut(s) 626, 1058
BanI GGYRCC 1 cut(s) 1032
BanII GRGCYC 3 cut(s) 242, 674, 1106
BbsI GAAGAC 2 cut(s) 425, 857
BbvI GCAGC 4 cut(s) 177, 337, 769, 1201
BccI CCATC 8 cut(s) 158, 284, 436, 590, 716, 868, 1022, 1148
BceAI ACGGC 3 cut(s) 127, 572, 1004
BciT130I CCWGG 6 cut(s) 131, 212, 563, 644, 995, 1076
BciVI GTATCC 3 cut(s) 169, 601, 1033
BcoDI GTCTC 2 cut(s) 401, 833
BfaI CTAG 3 cut(s) 320, 752, 1184
BfmI CTRYAG 2 cut(s) 427, 859
BfuAI ACCTGC 3 cut(s) 275, 707, 1139
BfuI GTATCC 3 cut(s) 169, 601, 1033
Bme1390I CCNGG 6 cut(s) 131, 212, 563, 644, 995, 1076
Bme18I GGWCC 2 cut(s) 168, 600
BmgT120I GGNCC 2 cut(s) 168, 600
BmiI GGNNCC 9 cut(s) 169, 170, 241, 601, 602, 673, 1034, 1105, 1282
BmrFI CCNGG 6 cut(s) 131, 212, 563, 644, 995, 1076
BmsI GCATC 3 cut(s) 177, 609, 1041
BpiI GAAGAC 2 cut(s) 425, 857
Bpu14I TTCGAA 3 cut(s) 362, 794, 1226
BsaJI CCNNGG 5 cut(s) 280, 712, 809, 1144, 1284
Bsc4I CCNNNNNNNGG 6 cut(s) 183, 235, 615, 667, 1047, 1099
BseBI CCWGG 6 cut(s) 131, 212, 563, 644, 995, 1076
BseDI CCNNGG 5 cut(s) 280, 712, 809, 1144, 1284
BseGI GGATG 9 cut(s) 192, 276, 319, 624, 708, 726, 751, 1056, 1140
BseLI CCNNNNNNNGG 6 cut(s) 183, 235, 615, 667, 1047, 1099
BseMII CTCAG 4 cut(s) 396, 644, 828, 1076
BseXI GCAGC 4 cut(s) 177, 337, 769, 1201
BseYI CCCAGC 1 cut(s) 120
BsgI GTGCAG 1 cut(s) 1117
Bsh1236I CGCG 3 cut(s) 8, 449, 881
BshNI GGYRCC 1 cut(s) 1032
BsiSI CCGG 3 cut(s) 437, 869, 1218
BslFI GGGAC 2 cut(s) 154, 586
BslI CCNNNNNNNGG 6 cut(s) 183, 235, 615, 667, 1047, 1099
BsmAI GTCTC 2 cut(s) 401, 833
BsmFI GGGAC 2 cut(s) 154, 586
BsmI GAATGC 3 cut(s) 370, 802, 1234
Bsp119I TTCGAA 3 cut(s) 362, 794, 1226
Bsp1286I GDGCHC 3 cut(s) 242, 674, 1106
BspCNI CTCAG 4 cut(s) 397, 643, 829, 1075
BspFNI CGCG 3 cut(s) 8, 449, 881
BspLI GGNNCC 9 cut(s) 169, 170, 241, 601, 602, 673, 1034, 1105, 1282
BspMI ACCTGC 3 cut(s) 275, 707, 1139
BspT104I TTCGAA 3 cut(s) 362, 794, 1226
BspT107I GGYRCC 1 cut(s) 1032
BssECI CCNNGG 5 cut(s) 280, 712, 809, 1144, 1284
BssT1I CCWWGG 4 cut(s) 280, 712, 1144, 1284
Bst2UI CCWGG 6 cut(s) 131, 212, 563, 644, 995, 1076
Bst4CI ACNGT 6 cut(s) 144, 157, 431, 576, 863, 1008
BstAPI GCANNNNNTGC 3 cut(s) 187, 619, 1051
BstBI TTCGAA 3 cut(s) 362, 794, 1226
BstC8I GCNNGC 3 cut(s) 195, 624, 1056
BstDEI CTNAG 4 cut(s) 405, 630, 837, 1062
BstDSI CCRYGG 1 cut(s) 809
BstEII GGTNACC 1 cut(s) 151
BstENI CCTNNNNNAGG 3 cut(s) 233, 665, 1097
BstF5I GGATG 9 cut(s) 192, 276, 319, 624, 708, 726, 751, 1056, 1140
BstFNI CGCG 3 cut(s) 8, 449, 881
BstMAI GTCTC 2 cut(s) 401, 833
BstMWI GCNNNNNNNGC 9 cut(s) 76, 123, 126, 187, 446, 619, 878, 949, 1051
BstNI CCWGG 6 cut(s) 131, 212, 563, 644, 995, 1076
BstPI GGTNACC 1 cut(s) 151
BstSCI CCNGG 6 cut(s) 129, 210, 561, 642, 993, 1074
BstSFI CTRYAG 2 cut(s) 427, 859
BstUI CGCG 3 cut(s) 8, 449, 881
BstV1I GCAGC 4 cut(s) 177, 337, 769, 1201
BstV2I GAAGAC 2 cut(s) 425, 857
BstXI CCANNNNNNTGG 2 cut(s) 551, 983
BsuI GTATCC 3 cut(s) 169, 601, 1033
BtgI CCRYGG 1 cut(s) 809
BtgZI GCGATG 2 cut(s) 455, 887
BtsCI GGATG 9 cut(s) 192, 276, 319, 624, 708, 726, 751, 1056, 1140
BtsIMutI CAGTG 3 cut(s) 135, 567, 999
BveI ACCTGC 3 cut(s) 275, 707, 1139
Cac8I GCNNGC 3 cut(s) 195, 624, 1056
Cfr13I GGNCC 2 cut(s) 168, 600
CseI GACGC 3 cut(s) 14, 455, 887
Csp6I GTAC 4 cut(s) 145, 577, 1009, 1033
CviAII CATG 2 cut(s) 551, 983
CviQI GTAC 4 cut(s) 145, 577, 1009, 1033
DdeI CTNAG 4 cut(s) 405, 630, 837, 1062
EaeI YGGCCR 7 cut(s) 127, 559, 585, 624, 991, 1017, 1056
EciI GGCGGA 3 cut(s) 239, 671, 1103
Eco130I CCWWGG 4 cut(s) 280, 712, 1144, 1284
Eco24I GRGCYC 3 cut(s) 242, 674, 1106
Eco32I GATATC 3 cut(s) 337, 769, 1201
Eco47I GGWCC 2 cut(s) 168, 600
Eco57I CTGAAG 6 cut(s) 53, 215, 494, 647, 707, 1079
Eco91I GGTNACC 1 cut(s) 151
EcoNI CCTNNNNNAGG 3 cut(s) 233, 665, 1097
EcoO109I RGGNCCY 2 cut(s) 168, 600
EcoO65I GGTNACC 1 cut(s) 151
EcoRII CCWGG 6 cut(s) 129, 210, 561, 642, 993, 1074
EcoRV GATATC 3 cut(s) 337, 769, 1201
EcoT14I CCWWGG 4 cut(s) 280, 712, 1144, 1284
EcoT38I GRGCYC 3 cut(s) 242, 674, 1106
ErhI CCWWGG 4 cut(s) 280, 712, 1144, 1284
FaeI CATG 2 cut(s) 554, 986
FalI AAGNNNNNCTT 6 cut(s) 213, 245, 645, 677, 1077, 1109
FaqI GGGAC 2 cut(s) 154, 586
FatI CATG 2 cut(s) 550, 982
FokI GGATG 9 cut(s) 199, 263, 326, 631, 695, 713, 758, 1063, 1127
FriOI GRGCYC 3 cut(s) 242, 674, 1106
FspBI CTAG 3 cut(s) 320, 752, 1184
GsaI CCCAGC 1 cut(s) 124
HapII CCGG 3 cut(s) 437, 869, 1218
HgaI GACGC 3 cut(s) 14, 455, 887
Hin1II CATG 2 cut(s) 554, 986
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HinfI GANTC 2 cut(s) 256, 1120
HpaII CCGG 3 cut(s) 437, 869, 1218
HphI GGTGA 5 cut(s) 163, 191, 256, 688, 1120
Hpy166II GTNNAC 1 cut(s) 55
Hpy188I TCNGA 6 cut(s) 163, 234, 595, 666, 1027, 1098
Hpy188III TCNNGA 3 cut(s) 40, 481, 913
Hpy8I GTNNAC 1 cut(s) 55
Hpy99I CGWCG 3 cut(s) 46, 487, 919
HpyAV CCTTC 6 cut(s) 229, 239, 661, 671, 1093, 1103
HpyCH4III ACNGT 6 cut(s) 144, 157, 431, 576, 863, 1008
HpyCH4V TGCA 6 cut(s) 79, 368, 800, 952, 1134, 1232
HpyF10VI GCNNNNNNNGC 9 cut(s) 76, 123, 126, 187, 446, 619, 878, 949, 1051
HpyF3I CTNAG 4 cut(s) 405, 630, 837, 1062
Hsp92II CATG 2 cut(s) 554, 986
KflI GGGWCCC 2 cut(s) 168, 600
KpnI GGTACC 1 cut(s) 1036
LmnI GCTCC 3 cut(s) 245, 677, 1109
Lsp1109I GCAGC 4 cut(s) 177, 337, 769, 1201
LweI GCATC 3 cut(s) 177, 609, 1041
MaeI CTAG 3 cut(s) 320, 752, 1184
MaeIII GTNAC 4 cut(s) 133, 151, 565, 997
MboII GAAGA 4 cut(s) 46, 425, 487, 857
MhlI GDGCHC 3 cut(s) 242, 674, 1106
MlsI TGGCCA 2 cut(s) 626, 1058
MluCI AATT 6 cut(s) 260, 411, 692, 843, 1124, 1274
MluNI TGGCCA 2 cut(s) 626, 1058
MnlI CCTC 7 cut(s) 3, 13, 385, 444, 454, 876, 886
Mox20I TGGCCA 2 cut(s) 626, 1058
MroXI GAANNNNTTC 3 cut(s) 260, 692, 1124
MscI TGGCCA 2 cut(s) 626, 1058
MseI TTAA 1 cut(s) 324
MslI CAYNNNNRTG 2 cut(s) 549, 981
Msp20I TGGCCA 2 cut(s) 626, 1058
MspI CCGG 3 cut(s) 437, 869, 1218
MspR9I CCNGG 6 cut(s) 131, 212, 563, 644, 995, 1076
Mva1269I GAATGC 3 cut(s) 370, 802, 1234
MvaI CCWGG 6 cut(s) 131, 212, 563, 644, 995, 1076
MvnI CGCG 3 cut(s) 8, 449, 881
MwoI GCNNNNNNNGC 9 cut(s) 76, 123, 126, 187, 446, 619, 878, 949, 1051
NlaIII CATG 2 cut(s) 554, 986
NlaIV GGNNCC 9 cut(s) 169, 170, 241, 601, 602, 673, 1034, 1105, 1282
NmuCI GTSAC 4 cut(s) 133, 151, 565, 997
NspV TTCGAA 3 cut(s) 362, 794, 1226
PaqCI CACCTGC 3 cut(s) 275, 707, 1139
PcsI WCGNNNNNNNCGW 3 cut(s) 23, 464, 896
PctI GAATGC 3 cut(s) 370, 802, 1234
PdmI GAANNNNTTC 3 cut(s) 260, 692, 1124
PfeI GAWTC 2 cut(s) 256, 1120
PpuMI RGGWCCY 2 cut(s) 168, 600
PsiI TTATAA 3 cut(s) 416, 848, 1166
Psp5II RGGWCCY 2 cut(s) 168, 600
Psp6I CCWGG 6 cut(s) 129, 210, 561, 642, 993, 1074
PspEI GGTNACC 1 cut(s) 151
PspFI CCCAGC 1 cut(s) 120
PspGI CCWGG 6 cut(s) 129, 210, 561, 642, 993, 1074
PspN4I GGNNCC 9 cut(s) 169, 170, 241, 601, 602, 673, 1034, 1105, 1282
PspPI GGNCC 2 cut(s) 168, 600
PspPPI RGGWCCY 2 cut(s) 168, 600
PsrI GAACNNNNNNTAC 2 cut(s) 732, 764
RsaI GTAC 4 cut(s) 146, 578, 1010, 1034
RsaNI GTAC 4 cut(s) 145, 577, 1009, 1033
RseI CAYNNNNRTG 2 cut(s) 549, 981
SaqAI TTAA 1 cut(s) 324
Sau96I GGNCC 2 cut(s) 168, 600
ScrFI CCNGG 6 cut(s) 131, 212, 563, 644, 995, 1076
SduI GDGCHC 3 cut(s) 242, 674, 1106
SetI ASST 9 cut(s) 135, 231, 269, 567, 663, 701, 999, 1095, 1133
SfaNI GCATC 3 cut(s) 177, 609, 1041
SfcI CTRYAG 2 cut(s) 427, 859
SfuI TTCGAA 3 cut(s) 362, 794, 1226
SinI GGWCC 2 cut(s) 168, 600
SmiMI CAYNNNNRTG 2 cut(s) 549, 981
Sse9I AATT 6 cut(s) 260, 411, 692, 843, 1124, 1274
SspMI CTAG 3 cut(s) 320, 752, 1184
StyD4I CCNGG 6 cut(s) 129, 210, 561, 642, 993, 1074
StyI CCWWGG 4 cut(s) 280, 712, 1144, 1284
TaaI ACNGT 6 cut(s) 144, 157, 431, 576, 863, 1008
TaqI TCGA 5 cut(s) 362, 528, 794, 960, 1226
TasI AATT 6 cut(s) 260, 411, 692, 843, 1124, 1274
TfiI GAWTC 2 cut(s) 256, 1120
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TscAI CASTG 3 cut(s) 142, 574, 1006
TseFI GTSAC 4 cut(s) 133, 151, 565, 997
TseI GCWGC 4 cut(s) 190, 350, 782, 1214
Tsp45I GTSAC 4 cut(s) 133, 151, 565, 997
TspRI CASTG 3 cut(s) 142, 574, 1006
VpaK11BI GGWCC 2 cut(s) 168, 600
XagI CCTNNNNNAGG 3 cut(s) 233, 665, 1097
XmnI GAANNNNTTC 3 cut(s) 260, 692, 1124
XspI CTAG 3 cut(s) 320, 752, 1184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.