Rmu_sc0006577.1_g000009

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006577.1
OG0017447
Physical Location & Seq
Reverse (-)
53291 .. 55366
2076 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006577.1_g000009.1.cds

Sequence Viewer

Length: 858 bp
atggacccgaggctaacggagggggcgatactgaagatcacaacggagtttgttgacgacaactataagcctatcctgcaagtgatcgacttgaagcagatccacacacagcgcggcggaggccaggtcactgagcggtacagggtggtcatctccgatgggtcccacactgcgcaggggatactgagcactcagcagaacttcctggttcagcaaggccaccttcagaagggctccgttgtccgcctgaatcaattcacctgcaccaccatccaaggccgcaagatcatcattattgtcttattggatgttctagttgataactgtgatatcattggacaggctgccagattttcgaatgcactatcctcacggacaagacttgtggatcaaacaactgagacaattataagcgaaccctacagtcttccggcgatggacgcgcggctaacggagggggcgatgctgaacatcacgacggagaattttgacgacaactataagcctaccctgcaagtgatcgacttgaaggaaatccacacacatggcggccgccaggtcactgaacggtacagggtggccgtacccgatgagtcccacaccgagcaggggatgccggccactcagcagaacttcctggttcagcaaggccaccttcagaagggttccgttgtccgcctgaatcaattcacctgccccaccatccaaggccgcaagatcatcattataatctcattgaatgtgctagttgataaatgtgatatcattggacaggctgccagattttcgaatgcactatcccaagggacaatacttatagatcaaacaaccgagcaattcggttccttggggctataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon zf-CCHC

Protein Analysis

285

Amino Acids

31.65

Weight (kDa)

6.16

Isoelectric Point (pI)

28.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 410, 728
AarI CACCTGC 2 cut(s) 269, 701
Acc16I TGCGCA 1 cut(s) 174
Acc36I ACCTGC 2 cut(s) 269, 701
AccBSI CCGCTC 1 cut(s) 136
AccII CGCG 3 cut(s) 114, 443, 445
AclWI GGATC 2 cut(s) 94, 396
AcoI YGGCCR 3 cut(s) 550, 579, 618
AcsI RAATTY 1 cut(s) 484
AcuI CTGAAG 3 cut(s) 53, 209, 641
AfaI GTAC 3 cut(s) 140, 572, 585
AfiI CCNNNNNNNGG 3 cut(s) 229, 609, 661
AgsI TTSAA 3 cut(s) 94, 529, 739
AjnI CCWGG 4 cut(s) 123, 204, 555, 636
Alw21I GWGCWC 1 cut(s) 191
Alw26I GTCTC 1 cut(s) 395
AlwI GGATC 2 cut(s) 94, 396
Ama87I CYCGRG 1 cut(s) 7
AoxI GGCC 8 cut(s) 121, 217, 277, 550, 579, 618, 649, 709
ApeKI GCWGC 2 cut(s) 344, 776
ApoI RAATTY 1 cut(s) 484
Asp700I GAANNNNTTC 2 cut(s) 254, 686
AspLEI GCGC 3 cut(s) 114, 175, 445
AspS9I GGNCC 2 cut(s) 4, 162
AsuHPI GGTGA 2 cut(s) 250, 682
AsuII TTCGAA 2 cut(s) 356, 788
AvaI CYCGRG 1 cut(s) 7
AvaII GGWCC 2 cut(s) 4, 162
BanII GRGCYC 1 cut(s) 236
BbsI GAAGAC 1 cut(s) 419
Bbv12I GWGCWC 1 cut(s) 191
BbvI GCAGC 2 cut(s) 331, 763
BccI CCATC 4 cut(s) 152, 278, 430, 710
BceAI ACGGC 1 cut(s) 566
BciT130I CCWGG 4 cut(s) 125, 206, 557, 638
BciVI GTATCC 1 cut(s) 174
BcoDI GTCTC 1 cut(s) 395
BfaI CTAG 2 cut(s) 314, 746
BfmI CTRYAG 1 cut(s) 421
BfuAI ACCTGC 2 cut(s) 269, 701
BfuI GTATCC 1 cut(s) 174
BisI GCNGC 8 cut(s) 115, 280, 345, 446, 550, 553, 712, 777
BlsI GCNGC 8 cut(s) 116, 281, 346, 447, 551, 554, 713, 778
Bme1390I CCNGG 4 cut(s) 125, 206, 557, 638
Bme18I GGWCC 2 cut(s) 4, 162
BmeT110I CYCGRG 1 cut(s) 7
BmgT120I GGNCC 2 cut(s) 4, 162
BmiI GGNNCC 6 cut(s) 6, 163, 164, 235, 667, 844
BmrFI CCNGG 4 cut(s) 125, 206, 557, 638
BmsI GCATC 2 cut(s) 453, 603
BpiI GAAGAC 1 cut(s) 419
Bpu14I TTCGAA 2 cut(s) 356, 788
BsaJI CCNNGG 5 cut(s) 8, 274, 706, 802, 846
BsaXI ACNNNNNCTCC 2 cut(s) 111, 141
Bsc4I CCNNNNNNNGG 3 cut(s) 229, 609, 661
Bse118I RCCGGY 1 cut(s) 616
BseBI CCWGG 4 cut(s) 125, 206, 557, 638
BseDI CCNNGG 5 cut(s) 8, 274, 706, 802, 846
BseGI GGATG 4 cut(s) 270, 313, 618, 702
BseLI CCNNNNNNNGG 3 cut(s) 229, 609, 661
BseMII CTCAG 5 cut(s) 123, 176, 206, 390, 638
BseX3I CGGCCG 1 cut(s) 550
BseXI GCAGC 2 cut(s) 331, 763
BsgI GTGCAG 1 cut(s) 247
Bsh1236I CGCG 3 cut(s) 114, 443, 445
Bsh1285I CGRYCG 1 cut(s) 553
BshFI GGCC 8 cut(s) 123, 219, 279, 552, 581, 620, 651, 711
BsiEI CGRYCG 1 cut(s) 553
BsiHKAI GWGCWC 1 cut(s) 191
BsiHKCI CYCGRG 1 cut(s) 7
BsiSI CCGG 2 cut(s) 431, 617
BslFI GGGAC 3 cut(s) 148, 580, 820
BslI CCNNNNNNNGG 3 cut(s) 229, 609, 661
BsmAI GTCTC 1 cut(s) 395
BsmFI GGGAC 3 cut(s) 148, 580, 820
BsmI GAATGC 2 cut(s) 364, 796
BsnI GGCC 8 cut(s) 123, 219, 279, 552, 581, 620, 651, 711
BsoBI CYCGRG 1 cut(s) 7
Bsp119I TTCGAA 2 cut(s) 356, 788
Bsp1286I GDGCHC 2 cut(s) 191, 236
Bsp143I GATC 8 cut(s) 36, 84, 99, 285, 388, 519, 717, 820
BspANI GGCC 8 cut(s) 123, 219, 279, 552, 581, 620, 651, 711
BspCNI CTCAG 5 cut(s) 124, 177, 205, 391, 637
BspFNI CGCG 3 cut(s) 114, 443, 445
BspLI GGNNCC 6 cut(s) 6, 163, 164, 235, 667, 844
BspMI ACCTGC 2 cut(s) 269, 701
BspPI GGATC 2 cut(s) 94, 396
BspT104I TTCGAA 2 cut(s) 356, 788
BsrBI CCGCTC 1 cut(s) 136
BsrFI RCCGGY 1 cut(s) 616
BssAI RCCGGY 1 cut(s) 616
BssECI CCNNGG 5 cut(s) 8, 274, 706, 802, 846
BssMI GATC 8 cut(s) 36, 84, 99, 285, 388, 519, 717, 820
BssT1I CCWWGG 4 cut(s) 274, 706, 802, 846
Bst2UI CCWGG 4 cut(s) 125, 206, 557, 638
Bst4CI ACNGT 3 cut(s) 326, 425, 570
BstAPI GCANNNNNTGC 1 cut(s) 613
BstBI TTCGAA 2 cut(s) 356, 788
BstC8I GCNNGC 1 cut(s) 618
BstDEI CTNAG 5 cut(s) 132, 185, 192, 399, 624
BstENI CCTNNNNNAGG 2 cut(s) 227, 659
BstF5I GGATG 4 cut(s) 270, 313, 618, 702
BstFNI CGCG 3 cut(s) 114, 443, 445
BstHHI GCGC 3 cut(s) 114, 175, 445
BstKTI GATC 8 cut(s) 39, 87, 102, 288, 391, 522, 720, 823
BstMAI GTCTC 1 cut(s) 395
BstMBI GATC 8 cut(s) 36, 84, 99, 285, 388, 519, 717, 820
BstMCI CGRYCG 1 cut(s) 553
BstMWI GCNNNNNNNGC 5 cut(s) 76, 120, 440, 511, 613
BstNI CCWGG 4 cut(s) 125, 206, 557, 638
BstSCI CCNGG 4 cut(s) 123, 204, 555, 636
BstSFI CTRYAG 1 cut(s) 421
BstUI CGCG 3 cut(s) 114, 443, 445
BstV1I GCAGC 2 cut(s) 331, 763
BstV2I GAAGAC 1 cut(s) 419
BstX2I RGATCY 1 cut(s) 99
BstXI CCANNNNNNTGG 1 cut(s) 545
BstYI RGATCY 1 cut(s) 99
BstZI CGGCCG 1 cut(s) 550
BsuI GTATCC 1 cut(s) 174
BsuRI GGCC 8 cut(s) 123, 219, 279, 552, 581, 620, 651, 711
BtgZI GCGATG 2 cut(s) 449, 476
BtsCI GGATG 4 cut(s) 270, 313, 618, 702
BtsI GCAGTG 1 cut(s) 168
BtsIMutI CAGTG 3 cut(s) 129, 168, 561
BveI ACCTGC 2 cut(s) 269, 701
Cac8I GCNNGC 1 cut(s) 618
CciNI GCGGCCGC 1 cut(s) 550
CfoI GCGC 3 cut(s) 114, 175, 445
Cfr10I RCCGGY 1 cut(s) 616
Cfr13I GGNCC 2 cut(s) 4, 162
CseI GACGC 1 cut(s) 449
Csp6I GTAC 3 cut(s) 139, 571, 584
CspCI CAANNNNNGTGG 2 cut(s) 366, 401
CviAII CATG 1 cut(s) 545
CviQI GTAC 3 cut(s) 139, 571, 584
DdeI CTNAG 5 cut(s) 132, 185, 192, 399, 624
DpnI GATC 8 cut(s) 38, 86, 101, 287, 390, 521, 719, 822
DpnII GATC 8 cut(s) 36, 84, 99, 285, 388, 519, 717, 820
EaeI YGGCCR 3 cut(s) 550, 579, 618
EagI CGGCCG 1 cut(s) 550
EciI GGCGGA 3 cut(s) 132, 233, 665
EclXI CGGCCG 1 cut(s) 550
Eco130I CCWWGG 4 cut(s) 274, 706, 802, 846
Eco24I GRGCYC 1 cut(s) 236
Eco32I GATATC 2 cut(s) 331, 763
Eco47I GGWCC 2 cut(s) 4, 162
Eco52I CGGCCG 1 cut(s) 550
Eco57I CTGAAG 3 cut(s) 53, 209, 641
Eco88I CYCGRG 1 cut(s) 7
EcoNI CCTNNNNNAGG 2 cut(s) 227, 659
EcoO109I RGGNCCY 1 cut(s) 162
EcoRII CCWGG 4 cut(s) 123, 204, 555, 636
EcoRV GATATC 2 cut(s) 331, 763
EcoT14I CCWWGG 4 cut(s) 274, 706, 802, 846
EcoT38I GRGCYC 1 cut(s) 236
ErhI CCWWGG 4 cut(s) 274, 706, 802, 846
FaeI CATG 1 cut(s) 548
FaiI YATR 7 cut(s) 66, 410, 501, 546, 728, 818, 856
FalI AAGNNNNNCTT 4 cut(s) 207, 239, 639, 671
FaqI GGGAC 3 cut(s) 148, 580, 820
FatI CATG 1 cut(s) 544
Fnu4HI GCNGC 8 cut(s) 115, 280, 345, 446, 550, 553, 712, 777
FokI GGATG 4 cut(s) 257, 320, 625, 689
FriOI GRGCYC 1 cut(s) 236
Fsp4HI GCNGC 8 cut(s) 115, 280, 345, 446, 550, 553, 712, 777
FspBI CTAG 2 cut(s) 314, 746
FspI TGCGCA 1 cut(s) 174
GlaI GCGC 3 cut(s) 113, 174, 444
GluI GCNGC 8 cut(s) 115, 280, 345, 446, 550, 553, 712, 777
HaeIII GGCC 8 cut(s) 123, 219, 279, 552, 581, 620, 651, 711
HapII CCGG 2 cut(s) 431, 617
HgaI GACGC 1 cut(s) 449
HhaI GCGC 3 cut(s) 114, 175, 445
Hin1II CATG 1 cut(s) 548
Hin6I GCGC 3 cut(s) 112, 173, 443
HinP1I GCGC 3 cut(s) 112, 173, 443
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HinfI GANTC 3 cut(s) 250, 593, 682
HpaII CCGG 2 cut(s) 431, 617
HphI GGTGA 2 cut(s) 250, 682
Hpy166II GTNNAC 1 cut(s) 55
Hpy188I TCNGA 3 cut(s) 157, 228, 660
Hpy188III TCNNGA 1 cut(s) 475
Hpy8I GTNNAC 1 cut(s) 55
Hpy99I CGWCG 1 cut(s) 481
HpyAV CCTTC 5 cut(s) 223, 233, 523, 655, 665
HpyCH4III ACNGT 3 cut(s) 326, 425, 570
HpyCH4V TGCA 5 cut(s) 79, 264, 362, 514, 794
HpyF10VI GCNNNNNNNGC 5 cut(s) 76, 120, 440, 511, 613
HpyF3I CTNAG 5 cut(s) 132, 185, 192, 399, 624
Hsp92II CATG 1 cut(s) 548
HspAI GCGC 3 cut(s) 112, 173, 443
KflI GGGWCCC 1 cut(s) 162
KroI GCCGGC 1 cut(s) 616
KroNI GCCGGC 1 cut(s) 618
Kzo9I GATC 8 cut(s) 36, 84, 99, 285, 388, 519, 717, 820
LmnI GCTCC 1 cut(s) 239
Lsp1109I GCAGC 2 cut(s) 331, 763
LweI GCATC 2 cut(s) 453, 603
MaeI CTAG 2 cut(s) 314, 746
MaeIII GTNAC 2 cut(s) 127, 559
MalI GATC 8 cut(s) 38, 86, 101, 287, 390, 521, 719, 822
MbiI CCGCTC 1 cut(s) 136
MboI GATC 8 cut(s) 36, 84, 99, 285, 388, 519, 717, 820
MboII GAAGA 2 cut(s) 46, 419
MflI RGATCY 1 cut(s) 99
MhlI GDGCHC 2 cut(s) 191, 236
MluCI AATT 5 cut(s) 254, 405, 484, 686, 836
MlyI GAGTC 1 cut(s) 602
MnlI CCTC 5 cut(s) 3, 13, 113, 379, 448
MroNI GCCGGC 1 cut(s) 616
MroXI GAANNNNTTC 2 cut(s) 254, 686
MslI CAYNNNNRTG 1 cut(s) 543
MspI CCGG 2 cut(s) 431, 617
MspR9I CCNGG 4 cut(s) 125, 206, 557, 638
Mva1269I GAATGC 2 cut(s) 364, 796
MvaI CCWGG 4 cut(s) 125, 206, 557, 638
MvnI CGCG 3 cut(s) 114, 443, 445
MwoI GCNNNNNNNGC 5 cut(s) 76, 120, 440, 511, 613
NaeI GCCGGC 1 cut(s) 618
NdeII GATC 8 cut(s) 36, 84, 99, 285, 388, 519, 717, 820
NgoMIV GCCGGC 1 cut(s) 616
NlaIII CATG 1 cut(s) 548
NlaIV GGNNCC 6 cut(s) 6, 163, 164, 235, 667, 844
NmuCI GTSAC 2 cut(s) 127, 559
NotI GCGGCCGC 1 cut(s) 550
NsbI TGCGCA 1 cut(s) 174
NspV TTCGAA 2 cut(s) 356, 788
PaqCI CACCTGC 2 cut(s) 269, 701
PcsI WCGNNNNNNNCGW 2 cut(s) 23, 458
PctI GAATGC 2 cut(s) 364, 796
PdiI GCCGGC 1 cut(s) 618
PdmI GAANNNNTTC 2 cut(s) 254, 686
PfeI GAWTC 2 cut(s) 250, 682
PkrI GCNGC 8 cut(s) 116, 281, 346, 447, 551, 554, 713, 778
PleI GAGTC 1 cut(s) 601
PpsI GAGTC 1 cut(s) 601
PpuMI RGGWCCY 1 cut(s) 162
PsiI TTATAA 2 cut(s) 410, 728
Psp5II RGGWCCY 1 cut(s) 162
Psp6I CCWGG 4 cut(s) 123, 204, 555, 636
PspGI CCWGG 4 cut(s) 123, 204, 555, 636
PspN4I GGNNCC 6 cut(s) 6, 163, 164, 235, 667, 844
PspPI GGNCC 2 cut(s) 4, 162
PspPPI RGGWCCY 1 cut(s) 162
PsuI RGATCY 1 cut(s) 99
RsaI GTAC 3 cut(s) 140, 572, 585
RsaNI GTAC 3 cut(s) 139, 571, 584
RseI CAYNNNNRTG 1 cut(s) 543
SatI GCNGC 8 cut(s) 115, 280, 345, 446, 550, 553, 712, 777
Sau3AI GATC 8 cut(s) 36, 84, 99, 285, 388, 519, 717, 820
Sau96I GGNCC 2 cut(s) 4, 162
SchI GAGTC 1 cut(s) 602
ScrFI CCNGG 4 cut(s) 125, 206, 557, 638
SduI GDGCHC 2 cut(s) 191, 236
SetI ASST 6 cut(s) 129, 225, 263, 561, 657, 695
SfaNI GCATC 2 cut(s) 453, 603
SfcI CTRYAG 1 cut(s) 421
SfuI TTCGAA 2 cut(s) 356, 788
SinI GGWCC 2 cut(s) 4, 162
SmiMI CAYNNNNRTG 1 cut(s) 543
Sse9I AATT 5 cut(s) 254, 405, 484, 686, 836
SspMI CTAG 2 cut(s) 314, 746
StyD4I CCNGG 4 cut(s) 123, 204, 555, 636
StyI CCWWGG 4 cut(s) 274, 706, 802, 846
TaaI ACNGT 3 cut(s) 326, 425, 570
TaqI TCGA 4 cut(s) 87, 356, 522, 788
TasI AATT 5 cut(s) 254, 405, 484, 686, 836
TauI GCSGC 6 cut(s) 117, 282, 448, 552, 555, 714
TfiI GAWTC 2 cut(s) 250, 682
TscAI CASTG 3 cut(s) 136, 175, 568
TseFI GTSAC 2 cut(s) 127, 559
TseI GCWGC 2 cut(s) 344, 776
Tsp45I GTSAC 2 cut(s) 127, 559
TspGWI ACGGA 7 cut(s) 32, 59, 226, 388, 467, 494, 658
TspRI CASTG 3 cut(s) 136, 175, 568
VpaK11BI GGWCC 2 cut(s) 4, 162
XagI CCTNNNNNAGG 2 cut(s) 227, 659
XapI RAATTY 1 cut(s) 484
XmnI GAANNNNTTC 2 cut(s) 254, 686
XspI CTAG 2 cut(s) 314, 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.