Rh7DG239900

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0017447
Physical Location & Seq
Reverse (-)
24582009 .. 24603551
21543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG239900.1

Sequence Viewer

Length: 1722 bp
ATGGACCCGAGGCTAACAGAGGGGGCGATACTGAAGATCACAACGGAGTTTGTTGACGACAACTATAAGCCTATCCTGCAAGTGATCGACTTGAAGCAGATCCACACACAGCGCGGCGGCGGCCAGGTCACTGAGCGGTACAGGGTGGTCATCTCCGATGGGTCCCACACTGCGCAGGGGATACTGAGCACTCAGCAGAACTTCCTGGTTCAGCAAGGCCACCTTCAGAAGGGCTCCTTTGTCTGCCTAAATCAATTCATCTGCACCACCATCCAAGGCCGCATGATCATCATTATTGTCTTATTGGATGTTCTAGTTAATAAATGTGATATCATTGGACAGGCTGCCAGATTTTCGAATGCACTATCCTCACGGACAAGACTTGTAGATCAAACAACTGAGACAATTATAAGCGAACCCTACAGTCTTCCGGCGATGGACGCGAGGCTAACGGAGGGGGCGATACTGAAGATCACGACGGAGTATTTTGACGACAACTATAAGCCTATCCTACAAGTGATCGACTTGAAGCAAATCCACACACATGGCGGCGGCCAGGTCACTGAACGGTACAGGGTGGCCGTATCCGATGGGTCCCACACCGAGCAGGGGATGCTGGCCACTCAGCAGAACTTCCTGGTTCAGCAAGGCCACCTTCAGAAGGGCTCCGTTGTCCGCCTGAAGCAATTCACCTGCCCCACCATCCAAGGCCGCAAGATCATCCTTATTGTCGTATTGGATGTTCTAGTTGATAAATGTGATATCATTGGACAGGCTGCCAGATTTTCGAATGCACTATCCCCACGGACAAGACTTGTAGATCAAACAACTGAGACAATTATAAGCGAACCCTACAGTCTTCCGGCGATGGACGCGAGGCTAACGGAGGGGGCGATACTGAACATCACGACGGAGTATTTTGACGACAACTATAAGCCTATCCTGCAAGTGATCGACTTGAAGCAAATCCACACACATGGCGGCGGCCAGGTCACTGAACGGTACAGGGTGGCCGTATCCGATGGGTACCACACCGAGCAGGGGATGCTGGCCACTCAGCAGAACTTCCTGGTTCAGCAAGGCCACCTTCAGAAGGGCTCCGTTGTCCGCCTGAATCAATTCACCTGCACCACCATCCAAGGCCGCAAGATCATCATTATAATCTCATTGAATGTGCTAGTTGATAAATGTGATATCATTGGACAGGCTGCCAGATTTTCGAATGCACTATCCCCACGGACAAGACTTGTAGATCAAACAACTGAGACAATTATAAGCGAACCCTACAGTCTTCCGGCGATGGACGCGAGGCTAACGGAGGGGGCGATACTGAACATCACGACGGAGTATTTTGACGACAACTATAAGCCTATCCTGCAAGTGATCGACTTGAAGCAAATCCACACACATGGCGGCGGCCAGGTCACTGAACGGTACAGGGTGGCCGTATCCGATGGGTACCACACCGAGCAGGGGATGCTGGCCACTCAGCAGAACTTCCTGGTTCAGCAAGGCCACCTTCAGAAGGGCTCCGTTGTCCGCCTGAATCAATTCACCTGCACCACCATCCAAGGCCGCAAGATCATCATTATAATCTCATTGAATGTGCTAGTTGATAAATGTGATATCATTGGACAGGCTGCCGGATTTTCGAATGCACTATCCCAATGGACAATACTTATAGATCAAACAACCGAGCAATTCGGTTCCTTGGGGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon zf-CCHC

Protein Analysis

573

Amino Acids

63.75

Weight (kDa)

6.44

Isoelectric Point (pI)

31.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 5 - 106 1.5e-25 Replication factor-A protein 1, N-terminal domain
Rep-A_N PF04057 150 - 250 1.3e-27 Replication factor-A protein 1, N-terminal domain
Rep-A_N PF04057 294 - 394 1.8e-28 Replication factor-A protein 1, N-terminal domain
Rep-A_N PF04057 438 - 538 1.8e-28 Replication factor-A protein 1, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 410, 842, 1160, 1274, 1592
AarI CACCTGC 3 cut(s) 701, 1133, 1565
Acc16I TGCGCA 1 cut(s) 174
Acc36I ACCTGC 3 cut(s) 701, 1133, 1565
Acc65I GGTACC 2 cut(s) 1026, 1458
AccB1I GGYRCC 2 cut(s) 1026, 1458
AccBSI CCGCTC 1 cut(s) 136
AccII CGCG 4 cut(s) 114, 443, 875, 1307
AclWI GGATC 1 cut(s) 94
AcuI CTGAAG 7 cut(s) 53, 209, 488, 641, 701, 1073, 1505
AfaI GTAC 6 cut(s) 140, 572, 1004, 1028, 1436, 1460
AfiI CCNNNNNNNGG 7 cut(s) 229, 609, 661, 1041, 1093, 1473, 1525
AgsI TTSAA 6 cut(s) 94, 529, 961, 1171, 1393, 1603
AjnI CCWGG 8 cut(s) 123, 204, 555, 636, 987, 1068, 1419, 1500
Alw21I GWGCWC 1 cut(s) 191
Alw26I GTCTC 3 cut(s) 395, 827, 1259
AlwI GGATC 1 cut(s) 94
Ama87I CYCGRG 1 cut(s) 7
ApeKI GCWGC 4 cut(s) 344, 776, 1208, 1640
Asp700I GAANNNNTTC 3 cut(s) 686, 1118, 1550
Asp718I GGTACC 2 cut(s) 1026, 1458
AspLEI GCGC 2 cut(s) 114, 175
AspS9I GGNCC 3 cut(s) 4, 162, 594
AsuHPI GGTGA 3 cut(s) 682, 1114, 1546
AsuII TTCGAA 4 cut(s) 356, 788, 1220, 1652
AvaI CYCGRG 1 cut(s) 7
AvaII GGWCC 3 cut(s) 4, 162, 594
BalI TGGCCA 3 cut(s) 620, 1052, 1484
BanI GGYRCC 2 cut(s) 1026, 1458
BanII GRGCYC 4 cut(s) 236, 668, 1100, 1532
BbsI GAAGAC 3 cut(s) 419, 851, 1283
Bbv12I GWGCWC 1 cut(s) 191
BbvI GCAGC 4 cut(s) 331, 763, 1195, 1627
BceAI ACGGC 3 cut(s) 566, 998, 1430
BciT130I CCWGG 8 cut(s) 125, 206, 557, 638, 989, 1070, 1421, 1502
BciVI GTATCC 4 cut(s) 174, 595, 1027, 1459
BclI TGATCA 1 cut(s) 285
BcoDI GTCTC 3 cut(s) 395, 827, 1259
BfaI CTAG 4 cut(s) 314, 746, 1178, 1610
BfmI CTRYAG 3 cut(s) 421, 853, 1285
BfuAI ACCTGC 3 cut(s) 701, 1133, 1565
BfuI GTATCC 4 cut(s) 174, 595, 1027, 1459
Bme1390I CCNGG 8 cut(s) 125, 206, 557, 638, 989, 1070, 1421, 1502
Bme18I GGWCC 3 cut(s) 4, 162, 594
BmeT110I CYCGRG 1 cut(s) 7
BmgT120I GGNCC 3 cut(s) 4, 162, 594
BmrFI CCNGG 8 cut(s) 125, 206, 557, 638, 989, 1070, 1421, 1502
BmsI GCATC 3 cut(s) 603, 1035, 1467
BpiI GAAGAC 3 cut(s) 419, 851, 1283
Bpu14I TTCGAA 4 cut(s) 356, 788, 1220, 1652
BsaJI CCNNGG 8 cut(s) 8, 274, 706, 803, 1138, 1235, 1570, 1710
Bsc4I CCNNNNNNNGG 7 cut(s) 229, 609, 661, 1041, 1093, 1473, 1525
BseBI CCWGG 8 cut(s) 125, 206, 557, 638, 989, 1070, 1421, 1502
BseDI CCNNGG 8 cut(s) 8, 274, 706, 803, 1138, 1235, 1570, 1710
BseLI CCNNNNNNNGG 7 cut(s) 229, 609, 661, 1041, 1093, 1473, 1525
BseMII CTCAG 9 cut(s) 123, 176, 206, 390, 638, 822, 1070, 1254, 1502
BseXI GCAGC 4 cut(s) 331, 763, 1195, 1627
BsgI GTGCAG 3 cut(s) 247, 1111, 1543
Bsh1236I CGCG 4 cut(s) 114, 443, 875, 1307
BshNI GGYRCC 2 cut(s) 1026, 1458
BsiHKAI GWGCWC 1 cut(s) 191
BsiHKCI CYCGRG 1 cut(s) 7
BsiSI CCGG 4 cut(s) 431, 863, 1295, 1644
BslFI GGGAC 2 cut(s) 148, 580
BslI CCNNNNNNNGG 7 cut(s) 229, 609, 661, 1041, 1093, 1473, 1525
BsmAI GTCTC 3 cut(s) 395, 827, 1259
BsmFI GGGAC 2 cut(s) 148, 580
BsmI GAATGC 4 cut(s) 364, 796, 1228, 1660
BsoBI CYCGRG 1 cut(s) 7
Bsp119I TTCGAA 4 cut(s) 356, 788, 1220, 1652
Bsp1286I GDGCHC 5 cut(s) 191, 236, 668, 1100, 1532
BspCNI CTCAG 9 cut(s) 124, 177, 205, 391, 637, 823, 1069, 1255, 1501
BspFNI CGCG 4 cut(s) 114, 443, 875, 1307
BspMI ACCTGC 3 cut(s) 701, 1133, 1565
BspPI GGATC 1 cut(s) 94
BspT104I TTCGAA 4 cut(s) 356, 788, 1220, 1652
BspT107I GGYRCC 2 cut(s) 1026, 1458
BsrBI CCGCTC 1 cut(s) 136
BssECI CCNNGG 8 cut(s) 8, 274, 706, 803, 1138, 1235, 1570, 1710
BssT1I CCWWGG 5 cut(s) 274, 706, 1138, 1570, 1710
Bst2UI CCWGG 8 cut(s) 125, 206, 557, 638, 989, 1070, 1421, 1502
Bst4CI ACNGT 6 cut(s) 425, 570, 857, 1002, 1289, 1434
BstAPI GCANNNNNTGC 3 cut(s) 613, 1045, 1477
BstBI TTCGAA 4 cut(s) 356, 788, 1220, 1652
BstC8I GCNNGC 3 cut(s) 618, 1050, 1482
BstDEI CTNAG 9 cut(s) 132, 185, 192, 399, 624, 831, 1056, 1263, 1488
BstDSI CCRYGG 2 cut(s) 803, 1235
BstENI CCTNNNNNAGG 4 cut(s) 227, 659, 1091, 1523
BstFNI CGCG 4 cut(s) 114, 443, 875, 1307
BstHHI GCGC 2 cut(s) 114, 175
BstMAI GTCTC 3 cut(s) 395, 827, 1259
BstNI CCWGG 8 cut(s) 125, 206, 557, 638, 989, 1070, 1421, 1502
BstSCI CCNGG 8 cut(s) 123, 204, 555, 636, 987, 1068, 1419, 1500
BstSFI CTRYAG 3 cut(s) 421, 853, 1285
BstUI CGCG 4 cut(s) 114, 443, 875, 1307
BstV1I GCAGC 4 cut(s) 331, 763, 1195, 1627
BstV2I GAAGAC 3 cut(s) 419, 851, 1283
BstX2I RGATCY 1 cut(s) 99
BstXI CCANNNNNNTGG 3 cut(s) 545, 977, 1409
BstYI RGATCY 1 cut(s) 99
BsuI GTATCC 4 cut(s) 174, 595, 1027, 1459
BtgI CCRYGG 2 cut(s) 803, 1235
BtgZI GCGATG 3 cut(s) 449, 881, 1313
BtsI GCAGTG 1 cut(s) 168
BtsIMutI CAGTG 5 cut(s) 129, 168, 561, 993, 1425
BveI ACCTGC 3 cut(s) 701, 1133, 1565
Cac8I GCNNGC 3 cut(s) 618, 1050, 1482
CfoI GCGC 2 cut(s) 114, 175
Cfr13I GGNCC 3 cut(s) 4, 162, 594
CseI GACGC 3 cut(s) 449, 881, 1313
Csp6I GTAC 6 cut(s) 139, 571, 1003, 1027, 1435, 1459
CviAII CATG 4 cut(s) 283, 545, 977, 1409
CviQI GTAC 6 cut(s) 139, 571, 1003, 1027, 1435, 1459
DdeI CTNAG 9 cut(s) 132, 185, 192, 399, 624, 831, 1056, 1263, 1488
EciI GGCGGA 3 cut(s) 665, 1097, 1529
Eco130I CCWWGG 5 cut(s) 274, 706, 1138, 1570, 1710
Eco24I GRGCYC 4 cut(s) 236, 668, 1100, 1532
Eco32I GATATC 4 cut(s) 331, 763, 1195, 1627
Eco47I GGWCC 3 cut(s) 4, 162, 594
Eco57I CTGAAG 7 cut(s) 53, 209, 488, 641, 701, 1073, 1505
Eco88I CYCGRG 1 cut(s) 7
EcoNI CCTNNNNNAGG 4 cut(s) 227, 659, 1091, 1523
EcoO109I RGGNCCY 2 cut(s) 162, 594
EcoRII CCWGG 8 cut(s) 123, 204, 555, 636, 987, 1068, 1419, 1500
EcoRV GATATC 4 cut(s) 331, 763, 1195, 1627
EcoT14I CCWWGG 5 cut(s) 274, 706, 1138, 1570, 1710
EcoT38I GRGCYC 4 cut(s) 236, 668, 1100, 1532
ErhI CCWWGG 5 cut(s) 274, 706, 1138, 1570, 1710
FaeI CATG 4 cut(s) 286, 548, 980, 1412
FaqI GGGAC 2 cut(s) 148, 580
FatI CATG 4 cut(s) 282, 544, 976, 1408
FbaI TGATCA 1 cut(s) 285
FriOI GRGCYC 4 cut(s) 236, 668, 1100, 1532
FspBI CTAG 4 cut(s) 314, 746, 1178, 1610
FspI TGCGCA 1 cut(s) 174
GlaI GCGC 2 cut(s) 113, 174
HapII CCGG 4 cut(s) 431, 863, 1295, 1644
HgaI GACGC 3 cut(s) 449, 881, 1313
HhaI GCGC 2 cut(s) 114, 175
Hin1II CATG 4 cut(s) 286, 548, 980, 1412
Hin6I GCGC 2 cut(s) 112, 173
HinP1I GCGC 2 cut(s) 112, 173
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HinfI GANTC 2 cut(s) 1114, 1546
HpaII CCGG 4 cut(s) 431, 863, 1295, 1644
HphI GGTGA 3 cut(s) 682, 1114, 1546
Hpy166II GTNNAC 1 cut(s) 55
Hpy188I TCNGA 8 cut(s) 157, 228, 589, 660, 1021, 1092, 1453, 1524
Hpy188III TCNNGA 3 cut(s) 475, 907, 1339
Hpy8I GTNNAC 1 cut(s) 55
Hpy99I CGWCG 3 cut(s) 481, 913, 1345
HpyAV CCTTC 8 cut(s) 223, 233, 655, 665, 1087, 1097, 1519, 1529
HpyCH4III ACNGT 6 cut(s) 425, 570, 857, 1002, 1289, 1434
HpyF3I CTNAG 9 cut(s) 132, 185, 192, 399, 624, 831, 1056, 1263, 1488
Hsp92II CATG 4 cut(s) 286, 548, 980, 1412
HspAI GCGC 2 cut(s) 112, 173
KflI GGGWCCC 2 cut(s) 162, 594
KpnI GGTACC 2 cut(s) 1030, 1462
Ksp22I TGATCA 1 cut(s) 285
LmnI GCTCC 4 cut(s) 239, 671, 1103, 1535
Lsp1109I GCAGC 4 cut(s) 331, 763, 1195, 1627
LweI GCATC 3 cut(s) 603, 1035, 1467
MaeI CTAG 4 cut(s) 314, 746, 1178, 1610
MaeIII GTNAC 4 cut(s) 127, 559, 991, 1423
MbiI CCGCTC 1 cut(s) 136
MboII GAAGA 5 cut(s) 46, 419, 481, 851, 1283
MflI RGATCY 1 cut(s) 99
MhlI GDGCHC 5 cut(s) 191, 236, 668, 1100, 1532
MlsI TGGCCA 3 cut(s) 620, 1052, 1484
MluCI AATT 8 cut(s) 254, 405, 686, 837, 1118, 1269, 1550, 1700
MluNI TGGCCA 3 cut(s) 620, 1052, 1484
MnlI CCTC 9 cut(s) 3, 13, 379, 438, 448, 870, 880, 1302, 1312
Mox20I TGGCCA 3 cut(s) 620, 1052, 1484
MroXI GAANNNNTTC 3 cut(s) 686, 1118, 1550
MscI TGGCCA 3 cut(s) 620, 1052, 1484
MseI TTAA 1 cut(s) 318
MslI CAYNNNNRTG 3 cut(s) 543, 975, 1407
Msp20I TGGCCA 3 cut(s) 620, 1052, 1484
MspI CCGG 4 cut(s) 431, 863, 1295, 1644
MspR9I CCNGG 8 cut(s) 125, 206, 557, 638, 989, 1070, 1421, 1502
Mva1269I GAATGC 4 cut(s) 364, 796, 1228, 1660
MvaI CCWGG 8 cut(s) 125, 206, 557, 638, 989, 1070, 1421, 1502
MvnI CGCG 4 cut(s) 114, 443, 875, 1307
NlaIII CATG 4 cut(s) 286, 548, 980, 1412
NmuCI GTSAC 4 cut(s) 127, 559, 991, 1423
NsbI TGCGCA 1 cut(s) 174
NspV TTCGAA 4 cut(s) 356, 788, 1220, 1652
PaqCI CACCTGC 3 cut(s) 701, 1133, 1565
PcsI WCGNNNNNNNCGW 3 cut(s) 458, 890, 1322
PctI GAATGC 4 cut(s) 364, 796, 1228, 1660
PdmI GAANNNNTTC 3 cut(s) 686, 1118, 1550
PfeI GAWTC 2 cut(s) 1114, 1546
PpuMI RGGWCCY 2 cut(s) 162, 594
PsiI TTATAA 5 cut(s) 410, 842, 1160, 1274, 1592
Psp5II RGGWCCY 2 cut(s) 162, 594
Psp6I CCWGG 8 cut(s) 123, 204, 555, 636, 987, 1068, 1419, 1500
PspGI CCWGG 8 cut(s) 123, 204, 555, 636, 987, 1068, 1419, 1500
PspPI GGNCC 3 cut(s) 4, 162, 594
PspPPI RGGWCCY 2 cut(s) 162, 594
PsrI GAACNNNNNNTAC 2 cut(s) 726, 758
PsuI RGATCY 1 cut(s) 99
RsaI GTAC 6 cut(s) 140, 572, 1004, 1028, 1436, 1460
RsaNI GTAC 6 cut(s) 139, 571, 1003, 1027, 1435, 1459
RseI CAYNNNNRTG 3 cut(s) 543, 975, 1407
SaqAI TTAA 1 cut(s) 318
Sau96I GGNCC 3 cut(s) 4, 162, 594
ScrFI CCNGG 8 cut(s) 125, 206, 557, 638, 989, 1070, 1421, 1502
SduI GDGCHC 5 cut(s) 191, 236, 668, 1100, 1532
SfaNI GCATC 3 cut(s) 603, 1035, 1467
SfcI CTRYAG 3 cut(s) 421, 853, 1285
SfuI TTCGAA 4 cut(s) 356, 788, 1220, 1652
SinI GGWCC 3 cut(s) 4, 162, 594
SmiMI CAYNNNNRTG 3 cut(s) 543, 975, 1407
Sse9I AATT 8 cut(s) 254, 405, 686, 837, 1118, 1269, 1550, 1700
SspMI CTAG 4 cut(s) 314, 746, 1178, 1610
StyD4I CCNGG 8 cut(s) 123, 204, 555, 636, 987, 1068, 1419, 1500
StyI CCWWGG 5 cut(s) 274, 706, 1138, 1570, 1710
TaaI ACNGT 6 cut(s) 425, 570, 857, 1002, 1289, 1434
TaqI TCGA 8 cut(s) 87, 356, 522, 788, 954, 1220, 1386, 1652
TasI AATT 8 cut(s) 254, 405, 686, 837, 1118, 1269, 1550, 1700
TfiI GAWTC 2 cut(s) 1114, 1546
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TscAI CASTG 5 cut(s) 136, 175, 568, 1000, 1432
TseFI GTSAC 4 cut(s) 127, 559, 991, 1423
TseI GCWGC 4 cut(s) 344, 776, 1208, 1640
Tsp45I GTSAC 4 cut(s) 127, 559, 991, 1423
TspDTI ATGAA 1 cut(s) 247
TspRI CASTG 5 cut(s) 136, 175, 568, 1000, 1432
VpaK11BI GGWCC 3 cut(s) 4, 162, 594
XagI CCTNNNNNAGG 4 cut(s) 227, 659, 1091, 1523
XmnI GAANNNNTTC 3 cut(s) 686, 1118, 1550
XspI CTAG 4 cut(s) 314, 746, 1178, 1610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.