RchiOBHm_Chr7g0207931

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0017447
Physical Location & Seq
Reverse (-)
25254644 .. 25258501
3858 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ18608

Sequence Viewer

Length: 1290 bp
ATGGACCCGAGGCTAACAGAGGGGGCGATACTGAAGATCACAACGGAGTTTGTTGACGACAACTATAAGCCTATCCTGCAAGTGATCGACTTGAAGCAGATCCACACACAGCGCGGCGGCGGCCAGGTCACTGAGCGGTACAGGGTGGTCATCTCCGATGGGTCCCACACTGCGCAGGGGATACTGAGCACTCAGCAGAACTTCCTGGTTCAGCAAGGCCACCTTCAGAAGGGCTCCTTTGTCTGCCTAAATCAATTCATCTGCACCACCATCCAAGGCCGCATGATCATCATTATTGTCTTATTGGATGTTCTAGTTAATAAATGTGATATCATTGGACAGGCTGCCAGATTTTCGAATGCACTATCCTCACGGACAAGACTTGTAGATCAAACAACTGAGACAATTATAAGCGAACCCTACAGTCTTCCGGCGATGGACGCGAGGCTAACGGAGGGGGCGATACTGAAGATCACGACGGAGTATTTTGACGACAACTATAAGCCTATCCTACAAGTGATCGACTTGAAGCAAATCCACACACATGGCGGCGGCCAGGTCACTGAACGGTACAGGGTGGCCGTATCCGATGGGTCCCACACCGAGCAGGGGATGCTGGCCACTCAGCAGAACTTCCTGGTTCAGCAAGGCCACCTTCAGAAGGGCTCCGTTGTCCGCCTGAAGCAATTCACCTGCCCCACCATCCAAGGCCGCAAGATCATCCTTATTGTCGTATTGGATGTTCTAGTTGATAAATGTGATATCATTGGACAGGCTGCCAGATTTTCGAATGCACTATCCCCACGGACAAGACTTGTAGATCAAACAACTGAGACAATTATAAGCGAACCCTACAGTCTTCCGGCGATGGACGCGAGGCTAACGGAGGGGGCGATACTGAACATCACGACGGAGTATTTTGACGACAACTATAAGCCTATCCTGCAAGTGATCGACTTGAAGCAAATCCACACACATGGCGGCGGCCAGGTCACTGAACGGTACAGGGTGGCCGTATCCGATGGGTACCACACCGAGCAGGGGATGCTGGCCACTCAGCAGAACTTCCTGGTTCAGCAAGGCCACCTTCAGAAGGGCTCCGTTGTCCGCCTGAATCAATTCACCTGCACCACCATCCAAGGCCGCAAGATCATCATTATAATCTCATTGAATGTGCTAGTTGATAAATGTGATATCATTGGACAGGCTGCCGGATTTTCGAATGCACTATCCCAATGGACAATACTTATAGATCAAACAACCGAGCAATTCGGTTCCTTGGGGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon zf-CCHC

Protein Analysis

429

Amino Acids

47.68

Weight (kDa)

6.42

Isoelectric Point (pI)

30.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 5 - 106 9.1e-26 Replication factor-A protein 1, N-terminal domain
Rep-A_N PF04057 150 - 250 8.2e-28 Replication factor-A protein 1, N-terminal domain
Rep-A_N PF04057 294 - 394 1.1e-28 Replication factor-A protein 1, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 410, 842, 1160
AarI CACCTGC 2 cut(s) 701, 1133
Acc16I TGCGCA 1 cut(s) 174
Acc36I ACCTGC 2 cut(s) 701, 1133
Acc65I GGTACC 1 cut(s) 1026
AccB1I GGYRCC 1 cut(s) 1026
AccBSI CCGCTC 1 cut(s) 136
AccII CGCG 3 cut(s) 114, 443, 875
AclWI GGATC 1 cut(s) 94
AcoI YGGCCR 7 cut(s) 121, 553, 579, 618, 985, 1011, 1050
AcuI CTGAAG 6 cut(s) 53, 209, 488, 641, 701, 1073
AfaI GTAC 4 cut(s) 140, 572, 1004, 1028
AfiI CCNNNNNNNGG 5 cut(s) 229, 609, 661, 1041, 1093
AgsI TTSAA 4 cut(s) 94, 529, 961, 1171
AjnI CCWGG 6 cut(s) 123, 204, 555, 636, 987, 1068
Alw21I GWGCWC 1 cut(s) 191
Alw26I GTCTC 2 cut(s) 395, 827
AlwI GGATC 1 cut(s) 94
Ama87I CYCGRG 1 cut(s) 7
ApeKI GCWGC 3 cut(s) 344, 776, 1208
Asp700I GAANNNNTTC 2 cut(s) 686, 1118
Asp718I GGTACC 1 cut(s) 1026
AspLEI GCGC 2 cut(s) 114, 175
AspS9I GGNCC 3 cut(s) 4, 162, 594
AsuHPI GGTGA 2 cut(s) 682, 1114
AsuII TTCGAA 3 cut(s) 356, 788, 1220
AvaI CYCGRG 1 cut(s) 7
AvaII GGWCC 3 cut(s) 4, 162, 594
BalI TGGCCA 2 cut(s) 620, 1052
BanI GGYRCC 1 cut(s) 1026
BanII GRGCYC 3 cut(s) 236, 668, 1100
BbsI GAAGAC 2 cut(s) 419, 851
Bbv12I GWGCWC 1 cut(s) 191
BbvI GCAGC 3 cut(s) 331, 763, 1195
BccI CCATC 8 cut(s) 152, 278, 430, 584, 710, 862, 1016, 1142
BceAI ACGGC 2 cut(s) 566, 998
BciT130I CCWGG 6 cut(s) 125, 206, 557, 638, 989, 1070
BciVI GTATCC 3 cut(s) 174, 595, 1027
BclI TGATCA 1 cut(s) 285
BcoDI GTCTC 2 cut(s) 395, 827
BfaI CTAG 3 cut(s) 314, 746, 1178
BfmI CTRYAG 2 cut(s) 421, 853
BfuAI ACCTGC 2 cut(s) 701, 1133
BfuI GTATCC 3 cut(s) 174, 595, 1027
Bme1390I CCNGG 6 cut(s) 125, 206, 557, 638, 989, 1070
Bme18I GGWCC 3 cut(s) 4, 162, 594
BmeT110I CYCGRG 1 cut(s) 7
BmgT120I GGNCC 3 cut(s) 4, 162, 594
BmrFI CCNGG 6 cut(s) 125, 206, 557, 638, 989, 1070
BmsI GCATC 2 cut(s) 603, 1035
BpiI GAAGAC 2 cut(s) 419, 851
Bpu14I TTCGAA 3 cut(s) 356, 788, 1220
BsaJI CCNNGG 6 cut(s) 8, 274, 706, 803, 1138, 1278
Bsc4I CCNNNNNNNGG 5 cut(s) 229, 609, 661, 1041, 1093
BseBI CCWGG 6 cut(s) 125, 206, 557, 638, 989, 1070
BseDI CCNNGG 6 cut(s) 8, 274, 706, 803, 1138, 1278
BseGI GGATG 8 cut(s) 270, 313, 618, 702, 720, 745, 1050, 1134
BseLI CCNNNNNNNGG 5 cut(s) 229, 609, 661, 1041, 1093
BseMII CTCAG 7 cut(s) 123, 176, 206, 390, 638, 822, 1070
BseXI GCAGC 3 cut(s) 331, 763, 1195
BsgI GTGCAG 2 cut(s) 247, 1111
Bsh1236I CGCG 3 cut(s) 114, 443, 875
BshNI GGYRCC 1 cut(s) 1026
BsiHKAI GWGCWC 1 cut(s) 191
BsiHKCI CYCGRG 1 cut(s) 7
BsiSI CCGG 3 cut(s) 431, 863, 1212
BslFI GGGAC 2 cut(s) 148, 580
BslI CCNNNNNNNGG 5 cut(s) 229, 609, 661, 1041, 1093
BsmAI GTCTC 2 cut(s) 395, 827
BsmFI GGGAC 2 cut(s) 148, 580
BsmI GAATGC 3 cut(s) 364, 796, 1228
BsoBI CYCGRG 1 cut(s) 7
Bsp119I TTCGAA 3 cut(s) 356, 788, 1220
Bsp1286I GDGCHC 4 cut(s) 191, 236, 668, 1100
BspCNI CTCAG 7 cut(s) 124, 177, 205, 391, 637, 823, 1069
BspFNI CGCG 3 cut(s) 114, 443, 875
BspMI ACCTGC 2 cut(s) 701, 1133
BspPI GGATC 1 cut(s) 94
BspT104I TTCGAA 3 cut(s) 356, 788, 1220
BspT107I GGYRCC 1 cut(s) 1026
BsrBI CCGCTC 1 cut(s) 136
BssECI CCNNGG 6 cut(s) 8, 274, 706, 803, 1138, 1278
BssT1I CCWWGG 4 cut(s) 274, 706, 1138, 1278
Bst2UI CCWGG 6 cut(s) 125, 206, 557, 638, 989, 1070
Bst4CI ACNGT 4 cut(s) 425, 570, 857, 1002
BstAPI GCANNNNNTGC 2 cut(s) 613, 1045
BstBI TTCGAA 3 cut(s) 356, 788, 1220
BstC8I GCNNGC 2 cut(s) 618, 1050
BstDEI CTNAG 7 cut(s) 132, 185, 192, 399, 624, 831, 1056
BstDSI CCRYGG 1 cut(s) 803
BstENI CCTNNNNNAGG 3 cut(s) 227, 659, 1091
BstF5I GGATG 8 cut(s) 270, 313, 618, 702, 720, 745, 1050, 1134
BstFNI CGCG 3 cut(s) 114, 443, 875
BstHHI GCGC 2 cut(s) 114, 175
BstMAI GTCTC 2 cut(s) 395, 827
BstMWI GCNNNNNNNGC 7 cut(s) 76, 120, 440, 613, 872, 943, 1045
BstNI CCWGG 6 cut(s) 125, 206, 557, 638, 989, 1070
BstSCI CCNGG 6 cut(s) 123, 204, 555, 636, 987, 1068
BstSFI CTRYAG 2 cut(s) 421, 853
BstUI CGCG 3 cut(s) 114, 443, 875
BstV1I GCAGC 3 cut(s) 331, 763, 1195
BstV2I GAAGAC 2 cut(s) 419, 851
BstX2I RGATCY 1 cut(s) 99
BstXI CCANNNNNNTGG 2 cut(s) 545, 977
BstYI RGATCY 1 cut(s) 99
BsuI GTATCC 3 cut(s) 174, 595, 1027
BtgI CCRYGG 1 cut(s) 803
BtgZI GCGATG 2 cut(s) 449, 881
BtsCI GGATG 8 cut(s) 270, 313, 618, 702, 720, 745, 1050, 1134
BtsI GCAGTG 1 cut(s) 168
BtsIMutI CAGTG 4 cut(s) 129, 168, 561, 993
BveI ACCTGC 2 cut(s) 701, 1133
Cac8I GCNNGC 2 cut(s) 618, 1050
CfoI GCGC 2 cut(s) 114, 175
Cfr13I GGNCC 3 cut(s) 4, 162, 594
CseI GACGC 2 cut(s) 449, 881
Csp6I GTAC 4 cut(s) 139, 571, 1003, 1027
CviAII CATG 3 cut(s) 283, 545, 977
CviQI GTAC 4 cut(s) 139, 571, 1003, 1027
DdeI CTNAG 7 cut(s) 132, 185, 192, 399, 624, 831, 1056
EaeI YGGCCR 7 cut(s) 121, 553, 579, 618, 985, 1011, 1050
EciI GGCGGA 2 cut(s) 665, 1097
Eco130I CCWWGG 4 cut(s) 274, 706, 1138, 1278
Eco24I GRGCYC 3 cut(s) 236, 668, 1100
Eco32I GATATC 3 cut(s) 331, 763, 1195
Eco47I GGWCC 3 cut(s) 4, 162, 594
Eco57I CTGAAG 6 cut(s) 53, 209, 488, 641, 701, 1073
Eco88I CYCGRG 1 cut(s) 7
EcoNI CCTNNNNNAGG 3 cut(s) 227, 659, 1091
EcoO109I RGGNCCY 2 cut(s) 162, 594
EcoRII CCWGG 6 cut(s) 123, 204, 555, 636, 987, 1068
EcoRV GATATC 3 cut(s) 331, 763, 1195
EcoT14I CCWWGG 4 cut(s) 274, 706, 1138, 1278
EcoT38I GRGCYC 3 cut(s) 236, 668, 1100
ErhI CCWWGG 4 cut(s) 274, 706, 1138, 1278
FaeI CATG 3 cut(s) 286, 548, 980
FalI AAGNNNNNCTT 8 cut(s) 207, 239, 221, 253, 639, 671, 1071, 1103
FaqI GGGAC 2 cut(s) 148, 580
FatI CATG 3 cut(s) 282, 544, 976
FbaI TGATCA 1 cut(s) 285
FokI GGATG 8 cut(s) 257, 320, 625, 689, 707, 752, 1057, 1121
FriOI GRGCYC 3 cut(s) 236, 668, 1100
FspBI CTAG 3 cut(s) 314, 746, 1178
FspI TGCGCA 1 cut(s) 174
GlaI GCGC 2 cut(s) 113, 174
HapII CCGG 3 cut(s) 431, 863, 1212
HgaI GACGC 2 cut(s) 449, 881
HhaI GCGC 2 cut(s) 114, 175
Hin1II CATG 3 cut(s) 286, 548, 980
Hin6I GCGC 2 cut(s) 112, 173
HinP1I GCGC 2 cut(s) 112, 173
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HinfI GANTC 1 cut(s) 1114
HpaII CCGG 3 cut(s) 431, 863, 1212
HphI GGTGA 2 cut(s) 682, 1114
Hpy166II GTNNAC 1 cut(s) 55
Hpy188I TCNGA 6 cut(s) 157, 228, 589, 660, 1021, 1092
Hpy188III TCNNGA 2 cut(s) 475, 907
Hpy8I GTNNAC 1 cut(s) 55
Hpy99I CGWCG 2 cut(s) 481, 913
HpyAV CCTTC 6 cut(s) 223, 233, 655, 665, 1087, 1097
HpyCH4III ACNGT 4 cut(s) 425, 570, 857, 1002
HpyCH4V TGCA 7 cut(s) 79, 264, 362, 794, 946, 1128, 1226
HpyF10VI GCNNNNNNNGC 7 cut(s) 76, 120, 440, 613, 872, 943, 1045
HpyF3I CTNAG 7 cut(s) 132, 185, 192, 399, 624, 831, 1056
Hsp92II CATG 3 cut(s) 286, 548, 980
HspAI GCGC 2 cut(s) 112, 173
KflI GGGWCCC 2 cut(s) 162, 594
KpnI GGTACC 1 cut(s) 1030
Ksp22I TGATCA 1 cut(s) 285
LmnI GCTCC 3 cut(s) 239, 671, 1103
Lsp1109I GCAGC 3 cut(s) 331, 763, 1195
LweI GCATC 2 cut(s) 603, 1035
MaeI CTAG 3 cut(s) 314, 746, 1178
MaeIII GTNAC 3 cut(s) 127, 559, 991
MbiI CCGCTC 1 cut(s) 136
MboII GAAGA 4 cut(s) 46, 419, 481, 851
MflI RGATCY 1 cut(s) 99
MhlI GDGCHC 4 cut(s) 191, 236, 668, 1100
MlsI TGGCCA 2 cut(s) 620, 1052
MluCI AATT 6 cut(s) 254, 405, 686, 837, 1118, 1268
MluNI TGGCCA 2 cut(s) 620, 1052
MnlI CCTC 7 cut(s) 3, 13, 379, 438, 448, 870, 880
Mox20I TGGCCA 2 cut(s) 620, 1052
MroXI GAANNNNTTC 2 cut(s) 686, 1118
MscI TGGCCA 2 cut(s) 620, 1052
MseI TTAA 1 cut(s) 318
MslI CAYNNNNRTG 2 cut(s) 543, 975
Msp20I TGGCCA 2 cut(s) 620, 1052
MspI CCGG 3 cut(s) 431, 863, 1212
MspR9I CCNGG 6 cut(s) 125, 206, 557, 638, 989, 1070
Mva1269I GAATGC 3 cut(s) 364, 796, 1228
MvaI CCWGG 6 cut(s) 125, 206, 557, 638, 989, 1070
MvnI CGCG 3 cut(s) 114, 443, 875
MwoI GCNNNNNNNGC 7 cut(s) 76, 120, 440, 613, 872, 943, 1045
NlaIII CATG 3 cut(s) 286, 548, 980
NmuCI GTSAC 3 cut(s) 127, 559, 991
NsbI TGCGCA 1 cut(s) 174
NspV TTCGAA 3 cut(s) 356, 788, 1220
PaqCI CACCTGC 2 cut(s) 701, 1133
PcsI WCGNNNNNNNCGW 2 cut(s) 458, 890
PctI GAATGC 3 cut(s) 364, 796, 1228
PdmI GAANNNNTTC 2 cut(s) 686, 1118
PfeI GAWTC 1 cut(s) 1114
PpuMI RGGWCCY 2 cut(s) 162, 594
PsiI TTATAA 3 cut(s) 410, 842, 1160
Psp5II RGGWCCY 2 cut(s) 162, 594
Psp6I CCWGG 6 cut(s) 123, 204, 555, 636, 987, 1068
PspGI CCWGG 6 cut(s) 123, 204, 555, 636, 987, 1068
PspPI GGNCC 3 cut(s) 4, 162, 594
PspPPI RGGWCCY 2 cut(s) 162, 594
PsrI GAACNNNNNNTAC 2 cut(s) 726, 758
PsuI RGATCY 1 cut(s) 99
RsaI GTAC 4 cut(s) 140, 572, 1004, 1028
RsaNI GTAC 4 cut(s) 139, 571, 1003, 1027
RseI CAYNNNNRTG 2 cut(s) 543, 975
SaqAI TTAA 1 cut(s) 318
Sau96I GGNCC 3 cut(s) 4, 162, 594
ScrFI CCNGG 6 cut(s) 125, 206, 557, 638, 989, 1070
SduI GDGCHC 4 cut(s) 191, 236, 668, 1100
SetI ASST 8 cut(s) 129, 225, 561, 657, 695, 993, 1089, 1127
SfaNI GCATC 2 cut(s) 603, 1035
SfcI CTRYAG 2 cut(s) 421, 853
SfuI TTCGAA 3 cut(s) 356, 788, 1220
SinI GGWCC 3 cut(s) 4, 162, 594
SmiMI CAYNNNNRTG 2 cut(s) 543, 975
Sse9I AATT 6 cut(s) 254, 405, 686, 837, 1118, 1268
SspMI CTAG 3 cut(s) 314, 746, 1178
StyD4I CCNGG 6 cut(s) 123, 204, 555, 636, 987, 1068
StyI CCWWGG 4 cut(s) 274, 706, 1138, 1278
TaaI ACNGT 4 cut(s) 425, 570, 857, 1002
TaqI TCGA 6 cut(s) 87, 356, 522, 788, 954, 1220
TasI AATT 6 cut(s) 254, 405, 686, 837, 1118, 1268
TfiI GAWTC 1 cut(s) 1114
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TscAI CASTG 4 cut(s) 136, 175, 568, 1000
TseFI GTSAC 3 cut(s) 127, 559, 991
TseI GCWGC 3 cut(s) 344, 776, 1208
Tsp45I GTSAC 3 cut(s) 127, 559, 991
TspDTI ATGAA 1 cut(s) 247
TspGWI ACGGA 9 cut(s) 59, 388, 467, 494, 658, 820, 899, 926, 1090
TspRI CASTG 4 cut(s) 136, 175, 568, 1000
VpaK11BI GGWCC 3 cut(s) 4, 162, 594
XagI CCTNNNNNAGG 3 cut(s) 227, 659, 1091
XmnI GAANNNNTTC 2 cut(s) 686, 1118
XspI CTAG 3 cut(s) 314, 746, 1178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.