Rh7CG249100

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0017447
Physical Location & Seq
Reverse (-)
23460233 .. 23475572
15340 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG249100.1

Sequence Viewer

Length: 858 bp
ATGGACGCGAGGCTAACGGAGGGGGCGATACTGAAGATCACGACGGACTTTGTTGACGACAACTATGAGCCTATCCTGCAAGTGATCGACTTGAAGCAGATCCACACACAGCACGGCGGCGGCGGCGGCACTGAGCGGTACAGGCTGGCCATGTCCGATGGGTCCTACATCCGGCAGGCGAATCTGGCCGCTCACCTGAACTTCCTGGTTCGGCAAGGCCACCTTCACAAGGGCTCCGTTGTCCGCCTGGATCAATTCACCTGCACCGCCATCCAAGGCCGCAAGATCATCCTTATTATCGTATTGGATGTTCTAGTTGATAAATGTGATATCATTGGACAGGCTGCCAGATTTTCGAATCCACTATCCCCACGGACAAGACTTGTAGATCAAACAACTGAGACAATTATAAGCGAACCCTACAGTCTTCCGGCGATGGACGCGAGGCTAACGGAGGGGGCGATACTGAACATCACGACGGAGTATTTTGACGACAACTATAAGCCTATCCTGCAAGTGATCGACTTGAAGCAAATCCACACACATGGCGGCGGCCAGGTCACTGAACGGTACAGGGTGGCCGTATCCGATGGGTACCACACCGAGCAGGGGATGCTGGCCACTCAGCAGAACTTCCTGGTTCAGCAAGGCCACCTTCAGAAGGGCTCCGTTGTCCGCCTGAATCAATTCACCTGCACCACCATCCAAGGCCGCAAGATCATCATTATAATCTCATTGAATGTGCTAGTTGATAAATGTGATATCATTGGACAGGCTGCCAGATTTTCGAATGCACTATCCCAAGGGACAACACTTATAGATCAAACAACCGAGCAATTCGGTTCCTTGGGGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon zf-CCHC

Protein Analysis

285

Amino Acids

31.57

Weight (kDa)

6.3

Isoelectric Point (pI)

30.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 5 - 106 1.3e-26 Replication factor-A protein 1, N-terminal domain
Rep-A_N PF04057 150 - 250 4.9e-29 Replication factor-A protein 1, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 410, 728
AarI CACCTGC 2 cut(s) 269, 701
Acc36I ACCTGC 2 cut(s) 269, 701
Acc65I GGTACC 1 cut(s) 594
AccB1I GGYRCC 1 cut(s) 594
AccBSI CCGCTC 2 cut(s) 136, 191
AccII CGCG 2 cut(s) 8, 443
AclWI GGATC 2 cut(s) 94, 258
AcoI YGGCCR 5 cut(s) 147, 186, 553, 579, 618
AcuI CTGAAG 2 cut(s) 53, 641
AfaI GTAC 3 cut(s) 140, 572, 596
AfiI CCNNNNNNNGG 4 cut(s) 171, 229, 609, 661
AgsI TTSAA 3 cut(s) 94, 529, 739
AjnI CCWGG 4 cut(s) 204, 246, 555, 636
Alw26I GTCTC 1 cut(s) 395
AlwI GGATC 2 cut(s) 94, 258
AoxI GGCC 9 cut(s) 147, 186, 217, 277, 553, 579, 618, 649, 709
ApeKI GCWGC 2 cut(s) 344, 776
Asp700I GAANNNNTTC 1 cut(s) 686
Asp718I GGTACC 1 cut(s) 594
AspS9I GGNCC 1 cut(s) 162
AsuHPI GGTGA 3 cut(s) 185, 250, 682
AsuII TTCGAA 2 cut(s) 356, 788
AvaII GGWCC 1 cut(s) 162
BalI TGGCCA 2 cut(s) 149, 620
BanI GGYRCC 1 cut(s) 594
BanII GRGCYC 2 cut(s) 236, 668
BbsI GAAGAC 1 cut(s) 419
BbvI GCAGC 2 cut(s) 331, 763
BccI CCATC 5 cut(s) 152, 278, 430, 584, 710
BceAI ACGGC 2 cut(s) 130, 566
BciT130I CCWGG 4 cut(s) 206, 248, 557, 638
BciVI GTATCC 1 cut(s) 595
BcoDI GTCTC 1 cut(s) 395
BfaI CTAG 2 cut(s) 314, 746
BfmI CTRYAG 1 cut(s) 421
BfuAI ACCTGC 2 cut(s) 269, 701
BfuI GTATCC 1 cut(s) 595
Bme1390I CCNGG 4 cut(s) 206, 248, 557, 638
Bme18I GGWCC 1 cut(s) 162
BmgT120I GGNCC 1 cut(s) 162
BmiI GGNNCC 5 cut(s) 163, 235, 596, 667, 844
BmrFI CCNGG 4 cut(s) 206, 248, 557, 638
BmsI GCATC 1 cut(s) 603
BpiI GAAGAC 1 cut(s) 419
Bpu14I TTCGAA 2 cut(s) 356, 788
BsaJI CCNNGG 5 cut(s) 274, 371, 706, 802, 846
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 4 cut(s) 171, 229, 609, 661
BseBI CCWGG 4 cut(s) 206, 248, 557, 638
BseDI CCNNGG 5 cut(s) 274, 371, 706, 802, 846
BseGI GGATG 6 cut(s) 168, 270, 288, 313, 618, 702
BseLI CCNNNNNNNGG 4 cut(s) 171, 229, 609, 661
BseMII CTCAG 3 cut(s) 123, 390, 638
BseXI GCAGC 2 cut(s) 331, 763
BsgI GTGCAG 2 cut(s) 247, 679
Bsh1236I CGCG 2 cut(s) 8, 443
BshFI GGCC 9 cut(s) 149, 188, 219, 279, 555, 581, 620, 651, 711
BshNI GGYRCC 1 cut(s) 594
BsiSI CCGG 2 cut(s) 172, 431
BslFI GGGAC 1 cut(s) 820
BslI CCNNNNNNNGG 4 cut(s) 171, 229, 609, 661
BsmAI GTCTC 1 cut(s) 395
BsmFI GGGAC 1 cut(s) 820
BsmI GAATGC 1 cut(s) 796
BsnI GGCC 9 cut(s) 149, 188, 219, 279, 555, 581, 620, 651, 711
Bsp119I TTCGAA 2 cut(s) 356, 788
Bsp1286I GDGCHC 2 cut(s) 236, 668
Bsp143I GATC 9 cut(s) 36, 84, 99, 250, 285, 388, 519, 717, 820
BspANI GGCC 9 cut(s) 149, 188, 219, 279, 555, 581, 620, 651, 711
BspCNI CTCAG 3 cut(s) 124, 391, 637
BspFNI CGCG 2 cut(s) 8, 443
BspLI GGNNCC 5 cut(s) 163, 235, 596, 667, 844
BspMI ACCTGC 2 cut(s) 269, 701
BspPI GGATC 2 cut(s) 94, 258
BspT104I TTCGAA 2 cut(s) 356, 788
BspT107I GGYRCC 1 cut(s) 594
BsrBI CCGCTC 2 cut(s) 136, 191
BssECI CCNNGG 5 cut(s) 274, 371, 706, 802, 846
BssMI GATC 9 cut(s) 36, 84, 99, 250, 285, 388, 519, 717, 820
BssT1I CCWWGG 4 cut(s) 274, 706, 802, 846
Bst2UI CCWGG 4 cut(s) 206, 248, 557, 638
Bst4CI ACNGT 2 cut(s) 425, 570
BstAPI GCANNNNNTGC 1 cut(s) 613
BstBI TTCGAA 2 cut(s) 356, 788
BstC8I GCNNGC 3 cut(s) 147, 177, 618
BstDEI CTNAG 3 cut(s) 132, 399, 624
BstDSI CCRYGG 1 cut(s) 371
BstENI CCTNNNNNAGG 2 cut(s) 227, 659
BstF5I GGATG 6 cut(s) 168, 270, 288, 313, 618, 702
BstFNI CGCG 2 cut(s) 8, 443
BstKTI GATC 9 cut(s) 39, 87, 102, 253, 288, 391, 522, 720, 823
BstMAI GTCTC 1 cut(s) 395
BstMBI GATC 9 cut(s) 36, 84, 99, 250, 285, 388, 519, 717, 820
BstMWI GCNNNNNNNGC 8 cut(s) 76, 123, 126, 142, 185, 440, 511, 613
BstNI CCWGG 4 cut(s) 206, 248, 557, 638
BstSCI CCNGG 4 cut(s) 204, 246, 555, 636
BstSFI CTRYAG 1 cut(s) 421
BstUI CGCG 2 cut(s) 8, 443
BstV1I GCAGC 2 cut(s) 331, 763
BstV2I GAAGAC 1 cut(s) 419
BstX2I RGATCY 1 cut(s) 99
BstXI CCANNNNNNTGG 1 cut(s) 545
BstYI RGATCY 1 cut(s) 99
BsuI GTATCC 1 cut(s) 595
BsuRI GGCC 9 cut(s) 149, 188, 219, 279, 555, 581, 620, 651, 711
BtgI CCRYGG 1 cut(s) 371
BtgZI GCGATG 1 cut(s) 449
BtsCI GGATG 6 cut(s) 168, 270, 288, 313, 618, 702
BtsIMutI CAGTG 2 cut(s) 129, 561
BveI ACCTGC 2 cut(s) 269, 701
Cac8I GCNNGC 3 cut(s) 147, 177, 618
Cfr13I GGNCC 1 cut(s) 162
CseI GACGC 2 cut(s) 14, 449
Csp6I GTAC 3 cut(s) 139, 571, 595
CviAII CATG 2 cut(s) 151, 545
CviQI GTAC 3 cut(s) 139, 571, 595
DdeI CTNAG 3 cut(s) 132, 399, 624
DpnI GATC 9 cut(s) 38, 86, 101, 252, 287, 390, 521, 719, 822
DpnII GATC 9 cut(s) 36, 84, 99, 250, 285, 388, 519, 717, 820
EaeI YGGCCR 5 cut(s) 147, 186, 553, 579, 618
EciI GGCGGA 2 cut(s) 233, 665
Eco130I CCWWGG 4 cut(s) 274, 706, 802, 846
Eco24I GRGCYC 2 cut(s) 236, 668
Eco32I GATATC 2 cut(s) 331, 763
Eco47I GGWCC 1 cut(s) 162
Eco57I CTGAAG 2 cut(s) 53, 641
EcoNI CCTNNNNNAGG 2 cut(s) 227, 659
EcoO109I RGGNCCY 1 cut(s) 162
EcoRII CCWGG 4 cut(s) 204, 246, 555, 636
EcoRV GATATC 2 cut(s) 331, 763
EcoT14I CCWWGG 4 cut(s) 274, 706, 802, 846
EcoT38I GRGCYC 2 cut(s) 236, 668
ErhI CCWWGG 4 cut(s) 274, 706, 802, 846
FaeI CATG 2 cut(s) 154, 548
FaiI YATR 8 cut(s) 66, 152, 410, 501, 546, 728, 818, 856
FalI AAGNNNNNCTT 4 cut(s) 207, 239, 639, 671
FaqI GGGAC 1 cut(s) 820
FatI CATG 2 cut(s) 150, 544
FokI GGATG 6 cut(s) 155, 257, 275, 320, 625, 689
FriOI GRGCYC 2 cut(s) 236, 668
FspBI CTAG 2 cut(s) 314, 746
HaeIII GGCC 9 cut(s) 149, 188, 219, 279, 555, 581, 620, 651, 711
HapII CCGG 2 cut(s) 172, 431
HgaI GACGC 2 cut(s) 14, 449
Hin1II CATG 2 cut(s) 154, 548
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HinfI GANTC 3 cut(s) 181, 358, 682
HpaII CCGG 2 cut(s) 172, 431
HphI GGTGA 3 cut(s) 185, 250, 682
Hpy166II GTNNAC 1 cut(s) 55
Hpy188I TCNGA 3 cut(s) 157, 589, 660
Hpy188III TCNNGA 2 cut(s) 40, 475
Hpy8I GTNNAC 1 cut(s) 55
Hpy99I CGWCG 2 cut(s) 46, 481
HpyAV CCTTC 3 cut(s) 233, 655, 665
HpyCH4III ACNGT 2 cut(s) 425, 570
HpyCH4V TGCA 5 cut(s) 79, 264, 514, 696, 794
HpyF10VI GCNNNNNNNGC 8 cut(s) 76, 123, 126, 142, 185, 440, 511, 613
HpyF3I CTNAG 3 cut(s) 132, 399, 624
Hsp92II CATG 2 cut(s) 154, 548
KpnI GGTACC 1 cut(s) 598
Kzo9I GATC 9 cut(s) 36, 84, 99, 250, 285, 388, 519, 717, 820
LmnI GCTCC 2 cut(s) 239, 671
Lsp1109I GCAGC 2 cut(s) 331, 763
LweI GCATC 1 cut(s) 603
MaeI CTAG 2 cut(s) 314, 746
MaeIII GTNAC 1 cut(s) 559
MalI GATC 9 cut(s) 38, 86, 101, 252, 287, 390, 521, 719, 822
MbiI CCGCTC 2 cut(s) 136, 191
MboI GATC 9 cut(s) 36, 84, 99, 250, 285, 388, 519, 717, 820
MboII GAAGA 2 cut(s) 46, 419
MflI RGATCY 1 cut(s) 99
MhlI GDGCHC 2 cut(s) 236, 668
MlsI TGGCCA 2 cut(s) 149, 620
MluCI AATT 4 cut(s) 254, 405, 686, 836
MluNI TGGCCA 2 cut(s) 149, 620
MnlI CCTC 4 cut(s) 3, 13, 438, 448
Mox20I TGGCCA 2 cut(s) 149, 620
MroXI GAANNNNTTC 1 cut(s) 686
MscI TGGCCA 2 cut(s) 149, 620
MslI CAYNNNNRTG 1 cut(s) 543
Msp20I TGGCCA 2 cut(s) 149, 620
MspI CCGG 2 cut(s) 172, 431
MspR9I CCNGG 4 cut(s) 206, 248, 557, 638
Mva1269I GAATGC 1 cut(s) 796
MvaI CCWGG 4 cut(s) 206, 248, 557, 638
MvnI CGCG 2 cut(s) 8, 443
MwoI GCNNNNNNNGC 8 cut(s) 76, 123, 126, 142, 185, 440, 511, 613
NdeII GATC 9 cut(s) 36, 84, 99, 250, 285, 388, 519, 717, 820
NlaIII CATG 2 cut(s) 154, 548
NlaIV GGNNCC 5 cut(s) 163, 235, 596, 667, 844
NmuCI GTSAC 1 cut(s) 559
NspV TTCGAA 2 cut(s) 356, 788
PaqCI CACCTGC 2 cut(s) 269, 701
PcsI WCGNNNNNNNCGW 2 cut(s) 23, 458
PctI GAATGC 1 cut(s) 796
PdmI GAANNNNTTC 1 cut(s) 686
PfeI GAWTC 3 cut(s) 181, 358, 682
PpuMI RGGWCCY 1 cut(s) 162
PsiI TTATAA 2 cut(s) 410, 728
Psp5II RGGWCCY 1 cut(s) 162
Psp6I CCWGG 4 cut(s) 204, 246, 555, 636
PspGI CCWGG 4 cut(s) 204, 246, 555, 636
PspN4I GGNNCC 5 cut(s) 163, 235, 596, 667, 844
PspPI GGNCC 1 cut(s) 162
PspPPI RGGWCCY 1 cut(s) 162
PsrI GAACNNNNNNTAC 2 cut(s) 294, 326
PsuI RGATCY 1 cut(s) 99
RsaI GTAC 3 cut(s) 140, 572, 596
RsaNI GTAC 3 cut(s) 139, 571, 595
RseI CAYNNNNRTG 1 cut(s) 543
Sau3AI GATC 9 cut(s) 36, 84, 99, 250, 285, 388, 519, 717, 820
Sau96I GGNCC 1 cut(s) 162
ScrFI CCNGG 4 cut(s) 206, 248, 557, 638
SduI GDGCHC 2 cut(s) 236, 668
SetI ASST 6 cut(s) 198, 225, 263, 561, 657, 695
SfaNI GCATC 1 cut(s) 603
SfcI CTRYAG 1 cut(s) 421
SfuI TTCGAA 2 cut(s) 356, 788
SinI GGWCC 1 cut(s) 162
SmiMI CAYNNNNRTG 1 cut(s) 543
Sse9I AATT 4 cut(s) 254, 405, 686, 836
SspMI CTAG 2 cut(s) 314, 746
StyD4I CCNGG 4 cut(s) 204, 246, 555, 636
StyI CCWWGG 4 cut(s) 274, 706, 802, 846
TaaI ACNGT 2 cut(s) 425, 570
TaqI TCGA 4 cut(s) 87, 356, 522, 788
TasI AATT 4 cut(s) 254, 405, 686, 836
TauI GCSGC 9 cut(s) 120, 123, 126, 129, 191, 282, 552, 555, 714
TfiI GAWTC 3 cut(s) 181, 358, 682
TscAI CASTG 2 cut(s) 136, 568
TseFI GTSAC 1 cut(s) 559
TseI GCWGC 2 cut(s) 344, 776
Tsp45I GTSAC 1 cut(s) 559
TspGWI ACGGA 7 cut(s) 32, 59, 226, 388, 467, 494, 658
TspRI CASTG 2 cut(s) 136, 568
VpaK11BI GGWCC 1 cut(s) 162
XagI CCTNNNNNAGG 2 cut(s) 227, 659
XmnI GAANNNNTTC 1 cut(s) 686
XspI CTAG 2 cut(s) 314, 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.