Rmu_sc0000905.1_g000017

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000905.1
OG0017447
Physical Location & Seq
Forward (+)
62105 .. 63035
931 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000905.1_g000017.1.cds

Sequence Viewer

Length: 804 bp
atggacgcgaggctaacgaagggggcgatactgaagatcacgacggagtttgttgacgacaactataagcctatcctgcaagtgatctacttgaagcagatccactcacagcacggcggcggcggcggcggcactgagcggtacaggctggccatgtccgatgggtcctacatccggcaggcgaatctggccgctcacctgaacttcctggttcggcaagaccaccttcacaagggctccgttgtccgcctggatcaattcacctgcaccgccatccaaggccgcaaatggaagttgtttgctacagttgctgggtgcaggatcatcattaatgtgctagttgataaatgtgatatcattggacgggctgccagattttcgaattcacaatccccacggacaagacttgtagatcaaacaactgagacaattataagcgaaccctacagtcttccggcgatggacgcgaagctaacggagggggcgatactgaaaatcacgacggagtattttgacgacaactataagcctgtcctgcaagtgatcggcttgaagcaaatccacacacacggcggcggctggggaggccaggtcactgaacggtacagggtgaccgtatccgatgggtcccacaccaagcaggggatgctgatcgctcagcagaacttcctggttcagcaaggccaccttcagaagggctccgttgtccgcctgaatgaattcacctgccccaccgtccaaggccgcaagtatgtgctagcttgtccttccttttcgaaatttgatgcttttgatcaagtttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon zf-CCHC

Protein Analysis

267

Amino Acids

29.77

Weight (kDa)

9.34

Isoelectric Point (pI)

35.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 434
AarI CACCTGC 2 cut(s) 272, 734
Acc36I ACCTGC 2 cut(s) 272, 734
AccBSI CCGCTC 2 cut(s) 139, 194
AccII CGCG 2 cut(s) 8, 467
AclWI GGATC 3 cut(s) 94, 261, 329
AcoI YGGCCR 2 cut(s) 150, 189
AcsI RAATTY 3 cut(s) 382, 719, 779
AcuI CTGAAG 2 cut(s) 53, 674
AfaI GTAC 2 cut(s) 143, 605
AfiI CCNNNNNNNGG 4 cut(s) 174, 232, 642, 694
AgsI TTSAA 2 cut(s) 94, 553
AjnI CCWGG 4 cut(s) 207, 249, 588, 669
AluBI AGCT 2 cut(s) 472, 761
AluI AGCT 2 cut(s) 472, 761
Alw26I GTCTC 1 cut(s) 419
AlwI GGATC 3 cut(s) 94, 261, 329
AlwNI CAGNNNCTG 1 cut(s) 311
AoxI GGCC 6 cut(s) 150, 189, 280, 586, 682, 742
ApeKI GCWGC 1 cut(s) 368
ApoI RAATTY 3 cut(s) 382, 719, 779
AseI ATTAAT 1 cut(s) 330
Asp700I GAANNNNTTC 1 cut(s) 719
AspS9I GGNCC 2 cut(s) 165, 627
AsuHPI GGTGA 4 cut(s) 188, 253, 622, 715
AsuII TTCGAA 2 cut(s) 380, 776
AsuNHI GCTAGC 1 cut(s) 757
AvaII GGWCC 2 cut(s) 165, 627
BalI TGGCCA 1 cut(s) 152
BanII GRGCYC 2 cut(s) 239, 701
BbsI GAAGAC 1 cut(s) 443
BbvI GCAGC 1 cut(s) 355
BccI CCATC 4 cut(s) 155, 281, 454, 617
BceAI ACGGC 2 cut(s) 130, 586
BciT130I CCWGG 4 cut(s) 209, 251, 590, 671
BciVI GTATCC 1 cut(s) 628
BclI TGATCA 1 cut(s) 793
BcoDI GTCTC 1 cut(s) 419
BfaI CTAG 2 cut(s) 338, 758
BfmI CTRYAG 2 cut(s) 303, 445
BfuAI ACCTGC 2 cut(s) 272, 734
BfuI GTATCC 1 cut(s) 628
BlpI GCTNAGC 1 cut(s) 657
Bme1390I CCNGG 4 cut(s) 209, 251, 590, 671
Bme18I GGWCC 2 cut(s) 165, 627
BmgT120I GGNCC 2 cut(s) 165, 627
BmiI GGNNCC 5 cut(s) 166, 238, 628, 629, 700
BmrFI CCNGG 4 cut(s) 209, 251, 590, 671
BmsI GCATC 2 cut(s) 636, 775
BmtI GCTAGC 1 cut(s) 761
BpiI GAAGAC 1 cut(s) 443
Bpu1102I GCTNAGC 1 cut(s) 657
Bpu14I TTCGAA 2 cut(s) 380, 776
BsaBI GATNNNNATC 1 cut(s) 650
BsaJI CCNNGG 3 cut(s) 277, 395, 739
BsaXI ACNNNNNCTCC 4 cut(s) 221, 251, 576, 606
Bsc4I CCNNNNNNNGG 4 cut(s) 174, 232, 642, 694
Bse8I GATNNNNATC 1 cut(s) 650
BseBI CCWGG 4 cut(s) 209, 251, 590, 671
BseDI CCNNGG 3 cut(s) 277, 395, 739
BseGI GGATG 3 cut(s) 171, 273, 651
BseJI GATNNNNATC 1 cut(s) 650
BseLI CCNNNNNNNGG 4 cut(s) 174, 232, 642, 694
BseMII CTCAG 3 cut(s) 126, 414, 671
BseXI GCAGC 1 cut(s) 355
BseYI CCCAGC 2 cut(s) 311, 579
BsgI GTGCAG 2 cut(s) 250, 337
Bsh1236I CGCG 2 cut(s) 8, 467
BshFI GGCC 6 cut(s) 152, 191, 282, 588, 684, 744
BsiSI CCGG 2 cut(s) 175, 455
BslFI GGGAC 1 cut(s) 613
BslI CCNNNNNNNGG 4 cut(s) 174, 232, 642, 694
BsmAI GTCTC 1 cut(s) 419
BsmFI GGGAC 1 cut(s) 613
BsnI GGCC 6 cut(s) 152, 191, 282, 588, 684, 744
Bsp119I TTCGAA 2 cut(s) 380, 776
Bsp1286I GDGCHC 2 cut(s) 239, 701
Bsp143I GATC 9 cut(s) 36, 84, 99, 253, 321, 412, 543, 651, 793
Bsp1720I GCTNAGC 1 cut(s) 657
BspANI GGCC 6 cut(s) 152, 191, 282, 588, 684, 744
BspCNI CTCAG 3 cut(s) 127, 415, 670
BspFNI CGCG 2 cut(s) 8, 467
BspLI GGNNCC 5 cut(s) 166, 238, 628, 629, 700
BspMI ACCTGC 2 cut(s) 272, 734
BspOI GCTAGC 1 cut(s) 761
BspPI GGATC 3 cut(s) 94, 261, 329
BspT104I TTCGAA 2 cut(s) 380, 776
BsrBI CCGCTC 2 cut(s) 139, 194
BssECI CCNNGG 3 cut(s) 277, 395, 739
BssMI GATC 9 cut(s) 36, 84, 99, 253, 321, 412, 543, 651, 793
BssT1I CCWWGG 2 cut(s) 277, 739
Bst2UI CCWGG 4 cut(s) 209, 251, 590, 671
Bst4CI ACNGT 5 cut(s) 307, 449, 603, 616, 736
BstAPI GCANNNNNTGC 1 cut(s) 646
BstBI TTCGAA 2 cut(s) 380, 776
BstC8I GCNNGC 3 cut(s) 150, 180, 759
BstDEI CTNAG 3 cut(s) 135, 423, 657
BstDSI CCRYGG 1 cut(s) 395
BstEII GGTNACC 1 cut(s) 610
BstENI CCTNNNNNAGG 2 cut(s) 230, 692
BstF5I GGATG 3 cut(s) 171, 273, 651
BstFNI CGCG 2 cut(s) 8, 467
BstKTI GATC 9 cut(s) 39, 87, 102, 256, 324, 415, 546, 654, 796
BstMAI GTCTC 1 cut(s) 419
BstMBI GATC 9 cut(s) 36, 84, 99, 253, 321, 412, 543, 651, 793
BstNI CCWGG 4 cut(s) 209, 251, 590, 671
BstPI GGTNACC 1 cut(s) 610
BstSCI CCNGG 4 cut(s) 207, 249, 588, 669
BstSFI CTRYAG 2 cut(s) 303, 445
BstUI CGCG 2 cut(s) 8, 467
BstV1I GCAGC 1 cut(s) 355
BstV2I GAAGAC 1 cut(s) 443
BstX2I RGATCY 1 cut(s) 99
BstYI RGATCY 1 cut(s) 99
BsuI GTATCC 1 cut(s) 628
BsuRI GGCC 6 cut(s) 152, 191, 282, 588, 684, 744
BtgI CCRYGG 1 cut(s) 395
BtgZI GCGATG 1 cut(s) 473
BtsCI GGATG 3 cut(s) 171, 273, 651
BtsIMutI CAGTG 2 cut(s) 132, 594
BveI ACCTGC 2 cut(s) 272, 734
Cac8I GCNNGC 3 cut(s) 150, 180, 759
CaiI CAGNNNCTG 1 cut(s) 311
Cfr13I GGNCC 2 cut(s) 165, 627
CseI GACGC 2 cut(s) 14, 473
Csp6I GTAC 2 cut(s) 142, 604
CviAII CATG 1 cut(s) 154
CviQI GTAC 2 cut(s) 142, 604
DdeI CTNAG 3 cut(s) 135, 423, 657
DpnI GATC 9 cut(s) 38, 86, 101, 255, 323, 414, 545, 653, 795
DpnII GATC 9 cut(s) 36, 84, 99, 253, 321, 412, 543, 651, 793
EaeI YGGCCR 2 cut(s) 150, 189
EciI GGCGGA 2 cut(s) 236, 698
Eco130I CCWWGG 2 cut(s) 277, 739
Eco24I GRGCYC 2 cut(s) 239, 701
Eco32I GATATC 1 cut(s) 355
Eco47I GGWCC 2 cut(s) 165, 627
Eco57I CTGAAG 2 cut(s) 53, 674
Eco91I GGTNACC 1 cut(s) 610
EcoNI CCTNNNNNAGG 2 cut(s) 230, 692
EcoO109I RGGNCCY 2 cut(s) 165, 627
EcoO65I GGTNACC 1 cut(s) 610
EcoRI GAATTC 2 cut(s) 382, 719
EcoRII CCWGG 4 cut(s) 207, 249, 588, 669
EcoRV GATATC 1 cut(s) 355
EcoT14I CCWWGG 2 cut(s) 277, 739
EcoT38I GRGCYC 2 cut(s) 239, 701
ErhI CCWWGG 2 cut(s) 277, 739
FaeI CATG 1 cut(s) 157
FaiI YATR 5 cut(s) 66, 155, 434, 525, 753
FalI AAGNNNNNCTT 4 cut(s) 210, 242, 672, 704
FaqI GGGAC 1 cut(s) 613
FatI CATG 1 cut(s) 153
FbaI TGATCA 1 cut(s) 793
FokI GGATG 3 cut(s) 158, 260, 658
FriOI GRGCYC 2 cut(s) 239, 701
FspBI CTAG 2 cut(s) 338, 758
GsaI CCCAGC 2 cut(s) 315, 583
HaeIII GGCC 6 cut(s) 152, 191, 282, 588, 684, 744
HapII CCGG 2 cut(s) 175, 455
HgaI GACGC 2 cut(s) 14, 473
Hin1II CATG 1 cut(s) 157
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HinfI GANTC 1 cut(s) 184
HpaII CCGG 2 cut(s) 175, 455
HphI GGTGA 4 cut(s) 188, 253, 622, 715
Hpy166II GTNNAC 1 cut(s) 55
Hpy188I TCNGA 3 cut(s) 160, 622, 693
Hpy188III TCNNGA 2 cut(s) 40, 499
Hpy8I GTNNAC 1 cut(s) 55
Hpy99I CGWCG 2 cut(s) 46, 505
HpyAV CCTTC 5 cut(s) 13, 236, 688, 698, 777
HpyCH4III ACNGT 5 cut(s) 307, 449, 603, 616, 736
HpyCH4V TGCA 4 cut(s) 79, 267, 318, 538
HpyF3I CTNAG 3 cut(s) 135, 423, 657
Hsp92II CATG 1 cut(s) 157
KflI GGGWCCC 1 cut(s) 627
Ksp22I TGATCA 1 cut(s) 793
Kzo9I GATC 9 cut(s) 36, 84, 99, 253, 321, 412, 543, 651, 793
LmnI GCTCC 2 cut(s) 242, 704
Lsp1109I GCAGC 1 cut(s) 355
LweI GCATC 2 cut(s) 636, 775
MaeI CTAG 2 cut(s) 338, 758
MaeIII GTNAC 2 cut(s) 592, 610
MalI GATC 9 cut(s) 38, 86, 101, 255, 323, 414, 545, 653, 795
MbiI CCGCTC 2 cut(s) 139, 194
MboI GATC 9 cut(s) 36, 84, 99, 253, 321, 412, 543, 651, 793
MboII GAAGA 2 cut(s) 46, 443
MflI RGATCY 1 cut(s) 99
MhlI GDGCHC 2 cut(s) 239, 701
MlsI TGGCCA 1 cut(s) 152
MluCI AATT 5 cut(s) 257, 382, 429, 719, 779
MluNI TGGCCA 1 cut(s) 152
MnlI CCTC 3 cut(s) 3, 472, 578
Mox20I TGGCCA 1 cut(s) 152
MroXI GAANNNNTTC 1 cut(s) 719
MscI TGGCCA 1 cut(s) 152
MseI TTAA 2 cut(s) 330, 802
MslI CAYNNNNRTG 1 cut(s) 332
Msp20I TGGCCA 1 cut(s) 152
MspI CCGG 2 cut(s) 175, 455
MspR9I CCNGG 4 cut(s) 209, 251, 590, 671
MvaI CCWGG 4 cut(s) 209, 251, 590, 671
MvnI CGCG 2 cut(s) 8, 467
NdeII GATC 9 cut(s) 36, 84, 99, 253, 321, 412, 543, 651, 793
NheI GCTAGC 1 cut(s) 757
NlaIII CATG 1 cut(s) 157
NlaIV GGNNCC 5 cut(s) 166, 238, 628, 629, 700
NmuCI GTSAC 2 cut(s) 592, 610
NspV TTCGAA 2 cut(s) 380, 776
PaqCI CACCTGC 2 cut(s) 272, 734
PcsI WCGNNNNNNNCGW 2 cut(s) 23, 482
PdmI GAANNNNTTC 1 cut(s) 719
PfeI GAWTC 1 cut(s) 184
PpuMI RGGWCCY 2 cut(s) 165, 627
PshBI ATTAAT 1 cut(s) 330
PsiI TTATAA 1 cut(s) 434
Psp5II RGGWCCY 2 cut(s) 165, 627
Psp6I CCWGG 4 cut(s) 207, 249, 588, 669
PspEI GGTNACC 1 cut(s) 610
PspFI CCCAGC 2 cut(s) 311, 579
PspGI CCWGG 4 cut(s) 207, 249, 588, 669
PspN4I GGNNCC 5 cut(s) 166, 238, 628, 629, 700
PspPI GGNCC 2 cut(s) 165, 627
PspPPI RGGWCCY 2 cut(s) 165, 627
PstNI CAGNNNCTG 1 cut(s) 311
PsuI RGATCY 1 cut(s) 99
RsaI GTAC 2 cut(s) 143, 605
RsaNI GTAC 2 cut(s) 142, 604
RseI CAYNNNNRTG 1 cut(s) 332
SaqAI TTAA 2 cut(s) 330, 802
Sau3AI GATC 9 cut(s) 36, 84, 99, 253, 321, 412, 543, 651, 793
Sau96I GGNCC 2 cut(s) 165, 627
ScrFI CCNGG 4 cut(s) 209, 251, 590, 671
SduI GDGCHC 2 cut(s) 239, 701
SetI ASST 8 cut(s) 201, 228, 266, 474, 594, 690, 728, 763
SfaNI GCATC 2 cut(s) 636, 775
SfcI CTRYAG 2 cut(s) 303, 445
SfuI TTCGAA 2 cut(s) 380, 776
SinI GGWCC 2 cut(s) 165, 627
SmiMI CAYNNNNRTG 1 cut(s) 332
Sse9I AATT 5 cut(s) 257, 382, 429, 719, 779
SspMI CTAG 2 cut(s) 338, 758
StyD4I CCNGG 4 cut(s) 207, 249, 588, 669
StyI CCWWGG 2 cut(s) 277, 739
TaaI ACNGT 5 cut(s) 307, 449, 603, 616, 736
TaqI TCGA 2 cut(s) 380, 776
TasI AATT 5 cut(s) 257, 382, 429, 719, 779
TfiI GAWTC 1 cut(s) 184
Tru1I TTAA 2 cut(s) 330, 802
Tru9I TTAA 2 cut(s) 330, 802
TscAI CASTG 2 cut(s) 139, 601
TseFI GTSAC 2 cut(s) 592, 610
TseI GCWGC 1 cut(s) 368
Tsp45I GTSAC 2 cut(s) 592, 610
TspDTI ATGAA 1 cut(s) 732
TspGWI ACGGA 6 cut(s) 59, 229, 412, 491, 518, 691
TspRI CASTG 2 cut(s) 139, 601
VpaK11BI GGWCC 2 cut(s) 165, 627
VspI ATTAAT 1 cut(s) 330
XagI CCTNNNNNAGG 2 cut(s) 230, 692
XapI RAATTY 3 cut(s) 382, 719, 779
XmnI GAANNNNTTC 1 cut(s) 719
XspI CTAG 2 cut(s) 338, 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.