Rh7BG229500

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0017447
Physical Location & Seq
Reverse (-)
20026461 .. 20032137
5677 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG229500.1

Sequence Viewer

Length: 1821 bp
ATGAACGCGAGGCTAACGAAGGGGGCGATACTGAAGATCACGACGGAGTTTGTTGACGACAACTATAAGCCTATCCTGCAAGTGATCTACATGAAGCAGATCCACACACAGCACGGCGTCGGCGGCGGCGCTGAGCGGTACAGGCTGGCCTGCCTGGCCATGTCCGATGGGTCCCACATCCGGCAGGCGATGCTGGCCGCTCACCTGAACTTCCTGGTTCGGCAAGGCCACCTTCACAAGGGCTCCGTTGTCCGCCTGGATCAATTCACCTGCACCGCCATCCAAGGCCGCAAGATCATCATTATTGTCTTATTGGATGTGCTAGTTGATAAATGTGATATCATTGGACGGGCTGCCAGATTTTCGAATGCACAATCCCCACGGACAAGACTTGTAGATCAAACAACTGAGACAAGTACAAGCGAACCCTTCAGTTTTCCGGCGATGGACGCGAGGCTAACGAAGGGGGCGATACTGAAGATCACGACGGAGTTTGTTGACGACAACTATAAGCCTATCCTGCAAGTGATCGACTTGAAGCAGATCCACACACCCCGCGTCGGCTACAGCGGCCTGGCCACTGAGCGGTACAGGGTGGTCATATCCGATGGGTCCCACACCGCGCAGGGGATACTGAGCACTCAGCAGAACTTCCTGGTTCAGCAAGGCCACCTTCAGAAGGGCTCCGTTGTCTGCCTAAATCAATTCATCTGCAACACCATCCAAGGCCGCAAGATCATCATTATTGTCTCATTGGATGTGCTAGTTGATAAATGTGATATCATTGGACAGGCTGCCAGATTTTCGAATGCACAATCCCCACGGACAAGACTTGTAGATCAAACAACCGAGCAATTCGGTTCTTCTACCCTCTCTTTCGCAACCCTCTCTCATTCCCGACCAACCAAACCCTTCAATTTTTCGGCGATGGACGCGAGGCTAACGGAGGGGGCGATACTGAAGATCACGACGGAGTTTGTTGACGACAACTATAAGCCTATCCTGCAAGTGATCGACTTGAAGCAGATCCACACACAGCGCGGCGGCGGCCAGGTCACTGAGCGGTACGTGGTGGTCATATCCGATGGGTCCCACACCGCACAGGGGATACTGAGCACTCAGCAGAACTTCCTGGTTCAGCAAGGCCACCTTCAGAAGGGCTCCGTTGTCTGCCTAAATCAATTCACCTGCACCACCATCCAAGGCCGCAAGATCATCATTAATGTCTTATTGGATGTGCTAGTTGATAAATGTGATATCATTGGACAGGCTGCCAGATTTTCGAATGCACTATCCCCACGGACAAGACTTGTAGATCAAACAACTGAGACAATTATAAGCGAACCCTACAGTCTTCCGGCGATGGACGCGAGGCTAACGGAGGGGGCGATACTGAACATCACGACGGAGTATTTTGACGACAACTATAAGCCTATCCTGCAAGTGATCGACAACTATAAGCCTATCCTGCAAGTGATCGACTTGAAGCAAATCCACACACACGGCGGCTGCGGCGGCCAGGTCACTGAACGGTACAGGGTGGCCATATCCGATGGGTCCCACACCGAGCAGGGGATGCTGGCCACTCAGCAGAACTTCCTGGTTCAGCAAGGCCACCTTCAGAAGGGCTCCGTTGTCCGCCTGAATCAATTCACCTGCACCACCATCCAAGGCCGCAAGATCATCATTATAATCTCATTGAATGTGCTAGTTGATAAATGTGATATCATTGGACAGGCTGCCAGATTTTCGAGTGCACAATCCCAAGGGACAATACTTATAGATCAAACAACCGAGCATTTCGGTTCCTTGGGGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon zf-CCHC

Protein Analysis

606

Amino Acids

67.0

Weight (kDa)

8.8

Isoelectric Point (pI)

30.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 5 - 109 2.6e-24 Replication factor-A protein 1, N-terminal domain
Rep-A_N PF04057 153 - 256 5.8e-27 Replication factor-A protein 1, N-terminal domain
Rep-A_N PF04057 314 - 415 4.8e-24 Replication factor-A protein 1, N-terminal domain
Rep-A_N PF04057 459 - 571 3.5e-26 Replication factor-A protein 1, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1337, 1691
AarI CACCTGC 3 cut(s) 278, 1196, 1664
Acc36I ACCTGC 3 cut(s) 278, 1196, 1664
AccBSI CCGCTC 4 cut(s) 136, 200, 586, 1063
AccII CGCG 7 cut(s) 8, 452, 558, 623, 935, 1041, 1370
AclWI GGATC 4 cut(s) 94, 267, 538, 1021
AcoI YGGCCR 7 cut(s) 156, 195, 576, 1048, 1516, 1542, 1581
AcuI CTGAAG 7 cut(s) 53, 415, 497, 659, 980, 1136, 1604
AcyI GRCGYC 1 cut(s) 117
AfaI GTAC 5 cut(s) 140, 418, 590, 1067, 1535
AgsI TTSAA 5 cut(s) 538, 916, 1021, 1486, 1702
AjnI CCWGG 9 cut(s) 153, 213, 255, 573, 654, 1050, 1131, 1518, 1599
Alw21I GWGCWC 3 cut(s) 641, 1118, 1759
Alw26I GTCTC 3 cut(s) 404, 754, 1322
Alw44I GTGCAC 1 cut(s) 1755
AlwI GGATC 4 cut(s) 94, 267, 538, 1021
ApaLI GTGCAC 1 cut(s) 1755
ApeKI GCWGC 5 cut(s) 353, 794, 1271, 1509, 1739
AseI ATTAAT 1 cut(s) 1221
Asp700I GAANNNNTTC 1 cut(s) 1649
AspLEI GCGC 3 cut(s) 131, 625, 1041
AspS9I GGNCC 4 cut(s) 171, 612, 1089, 1557
AsuHPI GGTGA 4 cut(s) 194, 259, 1177, 1645
AsuII TTCGAA 3 cut(s) 365, 806, 1283
AvaII GGWCC 4 cut(s) 171, 612, 1089, 1557
BaeGI GKGCMC 1 cut(s) 1759
BalI TGGCCA 4 cut(s) 158, 578, 1544, 1583
BanII GRGCYC 4 cut(s) 245, 686, 1163, 1631
BbsI GAAGAC 1 cut(s) 1346
Bbv12I GWGCWC 3 cut(s) 641, 1118, 1759
BbvI GCAGC 5 cut(s) 340, 781, 1258, 1496, 1726
BceAI ACGGC 2 cut(s) 130, 1519
BciT130I CCWGG 9 cut(s) 155, 215, 257, 575, 656, 1052, 1133, 1520, 1601
BciVI GTATCC 2 cut(s) 624, 1101
BcoDI GTCTC 3 cut(s) 404, 754, 1322
BfaI CTAG 4 cut(s) 323, 764, 1241, 1709
BfmI CTRYAG 2 cut(s) 565, 1348
BfoI RGCGCY 1 cut(s) 132
BfuAI ACCTGC 3 cut(s) 278, 1196, 1664
BfuI GTATCC 2 cut(s) 624, 1101
BglI GCCNNNNNGGC 1 cut(s) 155
BlpI GCTNAGC 1 cut(s) 132
Bme1390I CCNGG 9 cut(s) 155, 215, 257, 575, 656, 1052, 1133, 1520, 1601
Bme18I GGWCC 4 cut(s) 171, 612, 1089, 1557
BmgT120I GGNCC 4 cut(s) 171, 612, 1089, 1557
BmrFI CCNGG 9 cut(s) 155, 215, 257, 575, 656, 1052, 1133, 1520, 1601
BmsI GCATC 2 cut(s) 180, 1566
BpiI GAAGAC 1 cut(s) 1346
Bpu1102I GCTNAGC 1 cut(s) 132
Bpu14I TTCGAA 3 cut(s) 365, 806, 1283
BsaAI YACGTR 1 cut(s) 1069
BsaHI GRCGYC 1 cut(s) 117
BsaJI CCNNGG 9 cut(s) 283, 380, 724, 821, 1201, 1298, 1669, 1765, 1809
BsaXI ACNNNNNCTCC 2 cut(s) 227, 257
BseBI CCWGG 9 cut(s) 155, 215, 257, 575, 656, 1052, 1133, 1520, 1601
BseDI CCNNGG 9 cut(s) 283, 380, 724, 821, 1201, 1298, 1669, 1765, 1809
BseGI GGATG 9 cut(s) 177, 279, 322, 720, 763, 1197, 1240, 1581, 1665
BseSI GKGCMC 1 cut(s) 1759
BseXI GCAGC 5 cut(s) 340, 781, 1258, 1496, 1726
BsgI GTGCAG 3 cut(s) 256, 1174, 1642
Bsh1236I CGCG 7 cut(s) 8, 452, 558, 623, 935, 1041, 1370
BsiHKAI GWGCWC 3 cut(s) 641, 1118, 1759
BsiSI CCGG 3 cut(s) 181, 440, 1358
BslFI GGGAC 5 cut(s) 157, 598, 1075, 1543, 1783
BsmAI GTCTC 3 cut(s) 404, 754, 1322
BsmFI GGGAC 5 cut(s) 157, 598, 1075, 1543, 1783
BsmI GAATGC 3 cut(s) 373, 814, 1291
Bsp119I TTCGAA 3 cut(s) 365, 806, 1283
Bsp1286I GDGCHC 7 cut(s) 245, 641, 686, 1118, 1163, 1631, 1759
Bsp1720I GCTNAGC 1 cut(s) 132
BspFNI CGCG 7 cut(s) 8, 452, 558, 623, 935, 1041, 1370
BspMI ACCTGC 3 cut(s) 278, 1196, 1664
BspPI GGATC 4 cut(s) 94, 267, 538, 1021
BspT104I TTCGAA 3 cut(s) 365, 806, 1283
BsrBI CCGCTC 4 cut(s) 136, 200, 586, 1063
BssECI CCNNGG 9 cut(s) 283, 380, 724, 821, 1201, 1298, 1669, 1765, 1809
BssNI GRCGYC 1 cut(s) 117
BssT1I CCWWGG 6 cut(s) 283, 724, 1201, 1669, 1765, 1809
Bst2UI CCWGG 9 cut(s) 155, 215, 257, 575, 656, 1052, 1133, 1520, 1601
Bst4CI ACNGT 2 cut(s) 1352, 1533
BstACI GRCGYC 1 cut(s) 117
BstAPI GCANNNNNTGC 2 cut(s) 190, 1576
BstBAI YACGTR 1 cut(s) 1069
BstBI TTCGAA 3 cut(s) 365, 806, 1283
BstC8I GCNNGC 5 cut(s) 147, 151, 186, 195, 1581
BstDSI CCRYGG 3 cut(s) 380, 821, 1298
BstENI CCTNNNNNAGG 4 cut(s) 236, 677, 1154, 1622
BstF5I GGATG 9 cut(s) 177, 279, 322, 720, 763, 1197, 1240, 1581, 1665
BstFNI CGCG 7 cut(s) 8, 452, 558, 623, 935, 1041, 1370
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 3 cut(s) 131, 625, 1041
BstMAI GTCTC 3 cut(s) 404, 754, 1322
BstNI CCWGG 9 cut(s) 155, 215, 257, 575, 656, 1052, 1133, 1520, 1601
BstSCI CCNGG 9 cut(s) 153, 213, 255, 573, 654, 1050, 1131, 1518, 1599
BstSFI CTRYAG 2 cut(s) 565, 1348
BstSLI GKGCMC 1 cut(s) 1759
BstUI CGCG 7 cut(s) 8, 452, 558, 623, 935, 1041, 1370
BstV1I GCAGC 5 cut(s) 340, 781, 1258, 1496, 1726
BstV2I GAAGAC 1 cut(s) 1346
BstX2I RGATCY 3 cut(s) 99, 543, 1026
BstYI RGATCY 3 cut(s) 99, 543, 1026
BsuI GTATCC 2 cut(s) 624, 1101
BtgI CCRYGG 3 cut(s) 380, 821, 1298
BtgZI GCGATG 4 cut(s) 203, 458, 941, 1376
BtsCI GGATG 9 cut(s) 177, 279, 322, 720, 763, 1197, 1240, 1581, 1665
BtsIMutI CAGTG 3 cut(s) 579, 1056, 1524
BveI ACCTGC 3 cut(s) 278, 1196, 1664
Cac8I GCNNGC 5 cut(s) 147, 151, 186, 195, 1581
CfoI GCGC 3 cut(s) 131, 625, 1041
Cfr13I GGNCC 4 cut(s) 171, 612, 1089, 1557
CseI GACGC 5 cut(s) 106, 458, 547, 941, 1376
Csp6I GTAC 5 cut(s) 139, 417, 589, 1066, 1534
CviAII CATG 2 cut(s) 91, 160
CviQI GTAC 5 cut(s) 139, 417, 589, 1066, 1534
EaeI YGGCCR 7 cut(s) 156, 195, 576, 1048, 1516, 1542, 1581
EciI GGCGGA 2 cut(s) 242, 1628
Eco130I CCWWGG 6 cut(s) 283, 724, 1201, 1669, 1765, 1809
Eco24I GRGCYC 4 cut(s) 245, 686, 1163, 1631
Eco32I GATATC 4 cut(s) 340, 781, 1258, 1726
Eco47I GGWCC 4 cut(s) 171, 612, 1089, 1557
Eco57I CTGAAG 7 cut(s) 53, 415, 497, 659, 980, 1136, 1604
EcoNI CCTNNNNNAGG 4 cut(s) 236, 677, 1154, 1622
EcoO109I RGGNCCY 4 cut(s) 171, 612, 1089, 1557
EcoRII CCWGG 9 cut(s) 153, 213, 255, 573, 654, 1050, 1131, 1518, 1599
EcoRV GATATC 4 cut(s) 340, 781, 1258, 1726
EcoT14I CCWWGG 6 cut(s) 283, 724, 1201, 1669, 1765, 1809
EcoT38I GRGCYC 4 cut(s) 245, 686, 1163, 1631
ErhI CCWWGG 6 cut(s) 283, 724, 1201, 1669, 1765, 1809
FaeI CATG 2 cut(s) 94, 163
FalI AAGNNNNNCTT 8 cut(s) 216, 248, 657, 689, 1134, 1166, 1602, 1634
FaqI GGGAC 5 cut(s) 157, 598, 1075, 1543, 1783
FatI CATG 2 cut(s) 90, 159
FauI CCCGC 1 cut(s) 563
FokI GGATG 9 cut(s) 164, 266, 329, 707, 770, 1184, 1247, 1588, 1652
FriOI GRGCYC 4 cut(s) 245, 686, 1163, 1631
FspBI CTAG 4 cut(s) 323, 764, 1241, 1709
GlaI GCGC 3 cut(s) 130, 624, 1040
HaeII RGCGCY 1 cut(s) 132
HapII CCGG 3 cut(s) 181, 440, 1358
HgaI GACGC 5 cut(s) 106, 458, 547, 941, 1376
HhaI GCGC 3 cut(s) 131, 625, 1041
Hin1I GRCGYC 1 cut(s) 117
Hin1II CATG 2 cut(s) 94, 163
Hin6I GCGC 3 cut(s) 129, 623, 1039
HinP1I GCGC 3 cut(s) 129, 623, 1039
HincII GTYRAC 3 cut(s) 55, 499, 982
HindII GTYRAC 3 cut(s) 55, 499, 982
HinfI GANTC 1 cut(s) 1645
HpaII CCGG 3 cut(s) 181, 440, 1358
HphI GGTGA 4 cut(s) 194, 259, 1177, 1645
Hpy166II GTNNAC 4 cut(s) 55, 499, 982, 1757
Hpy188I TCNGA 7 cut(s) 166, 607, 678, 1084, 1155, 1552, 1623
Hpy188III TCNNGA 5 cut(s) 40, 484, 897, 967, 1402
Hpy8I GTNNAC 4 cut(s) 55, 499, 982, 1757
Hpy99I CGWCG 6 cut(s) 46, 122, 490, 563, 973, 1408
HpyCH4III ACNGT 2 cut(s) 1352, 1533
HpyCH4IV ACGT 1 cut(s) 1068
HpySE526I ACGT 1 cut(s) 1068
Hsp92I GRCGYC 1 cut(s) 117
Hsp92II CATG 2 cut(s) 94, 163
HspAI GCGC 3 cut(s) 129, 623, 1039
KflI GGGWCCC 4 cut(s) 171, 612, 1089, 1557
LmnI GCTCC 4 cut(s) 248, 689, 1166, 1634
Lsp1109I GCAGC 5 cut(s) 340, 781, 1258, 1496, 1726
LweI GCATC 2 cut(s) 180, 1566
MaeI CTAG 4 cut(s) 323, 764, 1241, 1709
MaeII ACGT 1 cut(s) 1068
MaeIII GTNAC 2 cut(s) 1054, 1522
MbiI CCGCTC 4 cut(s) 136, 200, 586, 1063
MboII GAAGA 5 cut(s) 46, 490, 855, 973, 1346
MflI RGATCY 3 cut(s) 99, 543, 1026
MhlI GDGCHC 7 cut(s) 245, 641, 686, 1118, 1163, 1631, 1759
MlsI TGGCCA 4 cut(s) 158, 578, 1544, 1583
MluCI AATT 7 cut(s) 263, 704, 854, 916, 1181, 1332, 1649
MluNI TGGCCA 4 cut(s) 158, 578, 1544, 1583
MnlI CCTC 8 cut(s) 3, 447, 881, 896, 930, 940, 1365, 1375
Mox20I TGGCCA 4 cut(s) 158, 578, 1544, 1583
MroXI GAANNNNTTC 1 cut(s) 1649
MscI TGGCCA 4 cut(s) 158, 578, 1544, 1583
MseI TTAA 1 cut(s) 1221
Msp20I TGGCCA 4 cut(s) 158, 578, 1544, 1583
MspA1I CMGCKG 1 cut(s) 570
MspI CCGG 3 cut(s) 181, 440, 1358
MspR9I CCNGG 9 cut(s) 155, 215, 257, 575, 656, 1052, 1133, 1520, 1601
Mva1269I GAATGC 3 cut(s) 373, 814, 1291
MvaI CCWGG 9 cut(s) 155, 215, 257, 575, 656, 1052, 1133, 1520, 1601
MvnI CGCG 7 cut(s) 8, 452, 558, 623, 935, 1041, 1370
NlaIII CATG 2 cut(s) 94, 163
NmuCI GTSAC 2 cut(s) 1054, 1522
NspV TTCGAA 3 cut(s) 365, 806, 1283
PaqCI CACCTGC 3 cut(s) 278, 1196, 1664
PcsI WCGNNNNNNNCGW 4 cut(s) 23, 467, 950, 1385
PctI GAATGC 3 cut(s) 373, 814, 1291
PdmI GAANNNNTTC 1 cut(s) 1649
PfeI GAWTC 1 cut(s) 1645
Ppu21I YACGTR 1 cut(s) 1069
PpuMI RGGWCCY 4 cut(s) 171, 612, 1089, 1557
PshBI ATTAAT 1 cut(s) 1221
PsiI TTATAA 2 cut(s) 1337, 1691
Psp5II RGGWCCY 4 cut(s) 171, 612, 1089, 1557
Psp6I CCWGG 9 cut(s) 153, 213, 255, 573, 654, 1050, 1131, 1518, 1599
PspGI CCWGG 9 cut(s) 153, 213, 255, 573, 654, 1050, 1131, 1518, 1599
PspPI GGNCC 4 cut(s) 171, 612, 1089, 1557
PspPPI RGGWCCY 4 cut(s) 171, 612, 1089, 1557
PsuI RGATCY 3 cut(s) 99, 543, 1026
RsaI GTAC 5 cut(s) 140, 418, 590, 1067, 1535
RsaNI GTAC 5 cut(s) 139, 417, 589, 1066, 1534
SaqAI TTAA 1 cut(s) 1221
Sau96I GGNCC 4 cut(s) 171, 612, 1089, 1557
ScrFI CCNGG 9 cut(s) 155, 215, 257, 575, 656, 1052, 1133, 1520, 1601
SduI GDGCHC 7 cut(s) 245, 641, 686, 1118, 1163, 1631, 1759
SfaNI GCATC 2 cut(s) 180, 1566
SfcI CTRYAG 2 cut(s) 565, 1348
SfiI GGCCNNNNNGGCC 1 cut(s) 155
SfuI TTCGAA 3 cut(s) 365, 806, 1283
SinI GGWCC 4 cut(s) 171, 612, 1089, 1557
Sse9I AATT 7 cut(s) 263, 704, 854, 916, 1181, 1332, 1649
SspMI CTAG 4 cut(s) 323, 764, 1241, 1709
StyD4I CCNGG 9 cut(s) 153, 213, 255, 573, 654, 1050, 1131, 1518, 1599
StyI CCWWGG 6 cut(s) 283, 724, 1201, 1669, 1765, 1809
TaaI ACNGT 2 cut(s) 1352, 1533
TaiI ACGT 1 cut(s) 1071
TaqI TCGA 8 cut(s) 365, 531, 806, 1014, 1283, 1449, 1479, 1751
TasI AATT 7 cut(s) 263, 704, 854, 916, 1181, 1332, 1649
TatI WGTACW 1 cut(s) 416
TfiI GAWTC 1 cut(s) 1645
Tru1I TTAA 1 cut(s) 1221
Tru9I TTAA 1 cut(s) 1221
TscAI CASTG 3 cut(s) 586, 1063, 1531
TseFI GTSAC 2 cut(s) 1054, 1522
TseI GCWGC 5 cut(s) 353, 794, 1271, 1509, 1739
Tsp45I GTSAC 2 cut(s) 1054, 1522
TspDTI ATGAA 3 cut(s) 17, 107, 697
TspRI CASTG 3 cut(s) 586, 1063, 1531
VneI GTGCAC 1 cut(s) 1755
VpaK11BI GGWCC 4 cut(s) 171, 612, 1089, 1557
VspI ATTAAT 1 cut(s) 1221
XagI CCTNNNNNAGG 4 cut(s) 236, 677, 1154, 1622
XmnI GAANNNNTTC 1 cut(s) 1649
XspI CTAG 4 cut(s) 323, 764, 1241, 1709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.