RchiOBHm_Chr7g0215331

Nnf1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0010244
Physical Location & Seq
Forward (+)
32937229 .. 32942211
4983 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 561 bp
ATGAGTCTCTGTTATTGTGGATTCAAAATCAGAAGAATCGGCGCGATGGGGACGAGGCAAAGGGACTTGAACAAGTCCTTCAACCTCGCCATACGTTCTCTCCTCGCTCCTTGCTCTTTTCAGGAATTCTGTGAAGCGCTGCCGAGTTTCACAGCTGCTGAGCATGAACGTCTTCACAGGCTCTTTACTCAGGTCATGAGTTCTTTGCATGAGAATGTAGAGGACGAGTTTCAGCATATATGTCTCGAAACACAGGTTGGAACTGCAATGGATACTGTAGAGCAACTGGTGGAAGAACAAAGTCTGGATCCTTTGTTTTCAAATAAGACTAATGTAATGGATGTTGCCCACGATCTGTCAAGAACAACAAAGAAGGAGATCCAGTACTTGACAGAGATGCTTGAAAAGGCGGAGGCACAGAATCACCTTAAGCGGGATCGTATTGAACTGTTAAAGAAAGGAAGACCAGATATGTCGGACATGGCAAATGCTGTTGAGAAGTTGAGAAGTGGGATTTCAAGTTATGGAATGTATAACAGCAATGGGGTCCATGATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nnf1

Protein Analysis

186

Amino Acids

21.24

Weight (kDa)

5.81

Isoelectric Point (pI)

45.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nnf1 PF03980 58 - 151 1.2e-08 Nnf1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 44
AciI CCGC 2 cut(s) 410, 433
AclWI GGATC 4 cut(s) 302, 315, 373, 444
AcsI RAATTY 1 cut(s) 125
AfaI GTAC 1 cut(s) 386
AfeI AGCGCT 1 cut(s) 138
AfiI CCNNNNNNNGG 1 cut(s) 433
AflII CTTAAG 1 cut(s) 428
AgsI TTSAA 7 cut(s) 25, 70, 82, 321, 404, 446, 519
AjuI GAANNNNNNNTTGG 2 cut(s) 240, 272
AluBI AGCT 1 cut(s) 155
AluI AGCT 1 cut(s) 155
Alw26I GTCTC 2 cut(s) 11, 248
AlwI GGATC 4 cut(s) 302, 315, 373, 444
AlwNI CAGNNNCTG 1 cut(s) 158
Aor51HI AGCGCT 1 cut(s) 138
ApeKI GCWGC 2 cut(s) 139, 155
ApoI RAATTY 1 cut(s) 125
Asp700I GAANNNNTTC 1 cut(s) 171
AspLEI GCGC 2 cut(s) 44, 139
AspS9I GGNCC 1 cut(s) 547
AsuHPI GGTGA 1 cut(s) 416
AvaII GGWCC 1 cut(s) 547
BamHI GGATCC 1 cut(s) 307
BbsI GAAGAC 2 cut(s) 164, 469
BbvI GCAGC 2 cut(s) 126, 142
BccI CCATC 1 cut(s) 40
BciVI GTATCC 1 cut(s) 265
BcoDI GTCTC 2 cut(s) 11, 248
BfmI CTRYAG 1 cut(s) 276
BfoI RGCGCY 1 cut(s) 140
BfrI CTTAAG 1 cut(s) 428
BfuI GTATCC 1 cut(s) 265
BisI GCNGC 2 cut(s) 140, 156
BlpI GCTNAGC 1 cut(s) 159
BlsI GCNGC 2 cut(s) 141, 157
BmcAI AGTACT 1 cut(s) 386
Bme18I GGWCC 1 cut(s) 547
BmgT120I GGNCC 1 cut(s) 547
BmiI GGNNCC 2 cut(s) 309, 548
BmsI GCATC 1 cut(s) 387
BpiI GAAGAC 2 cut(s) 164, 469
Bpu1102I GCTNAGC 1 cut(s) 159
BsaXI ACNNNNNCTCC 4 cut(s) 84, 114, 368, 398
Bsc4I CCNNNNNNNGG 1 cut(s) 433
Bse1I ACTGG 2 cut(s) 291, 382
Bse3DI GCAATG 2 cut(s) 273, 547
BseGI GGATG 1 cut(s) 346
BseLI CCNNNNNNNGG 1 cut(s) 433
BseMI GCAATG 2 cut(s) 273, 547
BseMII CTCAG 2 cut(s) 150, 203
BseNI ACTGG 2 cut(s) 291, 382
BseRI GAGGAG 1 cut(s) 92
BseXI GCAGC 2 cut(s) 126, 142
Bsh1236I CGCG 1 cut(s) 44
BslFI GGGAC 2 cut(s) 64, 77
BslI CCNNNNNNNGG 1 cut(s) 433
BsmAI GTCTC 2 cut(s) 11, 248
BsmFI GGGAC 2 cut(s) 64, 77
Bsp143I GATC 4 cut(s) 307, 352, 378, 436
Bsp1720I GCTNAGC 1 cut(s) 159
BspACI CCGC 2 cut(s) 410, 433
BspCNI CTCAG 2 cut(s) 151, 202
BspFNI CGCG 1 cut(s) 44
BspHI TCATGA 2 cut(s) 195, 557
BspLI GGNNCC 2 cut(s) 309, 548
BspPI GGATC 4 cut(s) 302, 315, 373, 444
BspTI CTTAAG 1 cut(s) 428
BsrDI GCAATG 2 cut(s) 273, 547
BsrI ACTGG 2 cut(s) 291, 382
BssMI GATC 4 cut(s) 307, 352, 378, 436
Bst4CI ACNGT 2 cut(s) 277, 450
BstAFI CTTAAG 1 cut(s) 428
BstDEI CTNAG 2 cut(s) 159, 189
BstF5I GGATG 1 cut(s) 346
BstFNI CGCG 1 cut(s) 44
BstH2I RGCGCY 1 cut(s) 140
BstHHI GCGC 2 cut(s) 44, 139
BstKTI GATC 4 cut(s) 310, 355, 381, 439
BstMAI GTCTC 2 cut(s) 11, 248
BstMBI GATC 4 cut(s) 307, 352, 378, 436
BstSFI CTRYAG 1 cut(s) 276
BstUI CGCG 1 cut(s) 44
BstV1I GCAGC 2 cut(s) 126, 142
BstV2I GAAGAC 2 cut(s) 164, 469
BstX2I RGATCY 2 cut(s) 307, 378
BstYI RGATCY 2 cut(s) 307, 378
BsuI GTATCC 1 cut(s) 265
BtgZI GCGATG 1 cut(s) 59
BtsCI GGATG 1 cut(s) 346
CaiI CAGNNNCTG 1 cut(s) 158
CciI TCATGA 2 cut(s) 195, 557
CfoI GCGC 2 cut(s) 44, 139
Cfr13I GGNCC 1 cut(s) 547
Csp6I GTAC 1 cut(s) 385
CviAII CATG 6 cut(s) 164, 196, 209, 481, 551, 558
CviJI RGCY 2 cut(s) 155, 181
CviKI_1 RGCY 2 cut(s) 155, 181
CviQI GTAC 1 cut(s) 385
DdeI CTNAG 2 cut(s) 159, 189
DpnI GATC 4 cut(s) 309, 354, 380, 438
DpnII GATC 4 cut(s) 307, 352, 378, 436
EciI GGCGGA 1 cut(s) 425
Eco47I GGWCC 1 cut(s) 547
Eco47III AGCGCT 1 cut(s) 138
EcoRI GAATTC 1 cut(s) 125
FaeI CATG 6 cut(s) 167, 199, 212, 484, 554, 561
FaqI GGGAC 2 cut(s) 64, 77
FatI CATG 6 cut(s) 163, 195, 208, 480, 550, 557
FauI CCCGC 1 cut(s) 426
Fnu4HI GCNGC 2 cut(s) 140, 156
FokI GGATG 1 cut(s) 353
Fsp4HI GCNGC 2 cut(s) 140, 156
GlaI GCGC 2 cut(s) 43, 138
GluI GCNGC 2 cut(s) 140, 156
HaeII RGCGCY 1 cut(s) 140
HhaI GCGC 2 cut(s) 44, 139
Hin1II CATG 6 cut(s) 167, 199, 212, 484, 554, 561
Hin6I GCGC 2 cut(s) 42, 137
HinP1I GCGC 2 cut(s) 42, 137
HinfI GANTC 5 cut(s) 4, 21, 36, 421, 554
HphI GGTGA 1 cut(s) 416
Hpy188I TCNGA 2 cut(s) 32, 478
Hpy188III TCNNGA 6 cut(s) 122, 196, 245, 305, 360, 558
HpyAV CCTTC 2 cut(s) 88, 367
HpyCH4III ACNGT 2 cut(s) 277, 450
HpyCH4IV ACGT 2 cut(s) 94, 169
HpyCH4V TGCA 2 cut(s) 208, 266
HpyF3I CTNAG 2 cut(s) 159, 189
HpySE526I ACGT 2 cut(s) 94, 169
Hsp92II CATG 6 cut(s) 167, 199, 212, 484, 554, 561
HspAI GCGC 2 cut(s) 42, 137
Kzo9I GATC 4 cut(s) 307, 352, 378, 436
LmnI GCTCC 1 cut(s) 112
LpnPI CCDG 8 cut(s) 107, 163, 176, 239, 272, 290, 395, 480
Lsp1109I GCAGC 2 cut(s) 126, 142
LweI GCATC 1 cut(s) 387
MaeII ACGT 2 cut(s) 94, 169
MalI GATC 4 cut(s) 309, 354, 380, 438
MboI GATC 4 cut(s) 307, 352, 378, 436
MboII GAAGA 4 cut(s) 45, 164, 305, 474
MflI RGATCY 2 cut(s) 307, 378
MluCI AATT 1 cut(s) 125
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 2 cut(s) 238, 456
MnlI CCTC 5 cut(s) 48, 95, 113, 214, 406
MroXI GAANNNNTTC 1 cut(s) 171
MseI TTAA 2 cut(s) 429, 452
MslI CAYNNNNRTG 1 cut(s) 213
MspA1I CMGCKG 1 cut(s) 155
MspCI CTTAAG 1 cut(s) 428
MvnI CGCG 1 cut(s) 44
NdeII GATC 4 cut(s) 307, 352, 378, 436
NlaIII CATG 6 cut(s) 167, 199, 212, 484, 554, 561
NlaIV GGNNCC 2 cut(s) 309, 548
NmeAIII GCCGAG 1 cut(s) 168
PagI TCATGA 2 cut(s) 195, 557
PdmI GAANNNNTTC 1 cut(s) 171
PfeI GAWTC 4 cut(s) 21, 36, 421, 554
PkrI GCNGC 2 cut(s) 141, 157
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
PspN4I GGNNCC 2 cut(s) 309, 548
PspPI GGNCC 1 cut(s) 547
PstNI CAGNNNCTG 1 cut(s) 158
PsuI RGATCY 2 cut(s) 307, 378
PvuII CAGCTG 1 cut(s) 155
RsaI GTAC 1 cut(s) 386
RsaNI GTAC 1 cut(s) 385
RseI CAYNNNNRTG 1 cut(s) 213
SaqAI TTAA 2 cut(s) 429, 452
SatI GCNGC 2 cut(s) 140, 156
Sau3AI GATC 4 cut(s) 307, 352, 378, 436
Sau96I GGNCC 1 cut(s) 547
ScaI AGTACT 1 cut(s) 386
SchI GAGTC 1 cut(s) 13
SetI ASST 7 cut(s) 87, 97, 157, 172, 195, 258, 429
SfaNI GCATC 1 cut(s) 387
SfcI CTRYAG 1 cut(s) 276
SinI GGWCC 1 cut(s) 547
SmiMI CAYNNNNRTG 1 cut(s) 213
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
Sse9I AATT 1 cut(s) 125
SsiI CCGC 2 cut(s) 410, 433
TaaI ACNGT 2 cut(s) 277, 450
TaiI ACGT 2 cut(s) 97, 172
TaqI TCGA 1 cut(s) 246
TasI AATT 1 cut(s) 125
TatI WGTACW 1 cut(s) 384
TfiI GAWTC 4 cut(s) 21, 36, 421, 554
Tru1I TTAA 2 cut(s) 429, 452
Tru9I TTAA 2 cut(s) 429, 452
TseI GCWGC 2 cut(s) 139, 155
TspDTI ATGAA 2 cut(s) 180, 546
Vha464I CTTAAG 1 cut(s) 428
VpaK11BI GGWCC 1 cut(s) 547
XapI RAATTY 1 cut(s) 125
XmnI GAANNNNTTC 1 cut(s) 171
ZrmI AGTACT 1 cut(s) 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.