RchiOBHm_Chr7g0242551

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0013581
Physical Location & Seq
Reverse (-)
68732823 .. 68759467
26645 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3345 bp
ATGTCAAATACCGCAGGTGTCTCCAATTCACCTGTTATGGAAGTTCTTAACCGTAATAACTATAAAAAGTGGAGATTGCGGGTAAAAACTTACTTGTTGGCCGAAGATTTATGGGAGGTTATCGAAGCAATCAGGGAACCTGTTACAAAATTTGATGACAACGAACCCGAATACAAGGCTTGGACAATAAAGAATGCTAAGGCTTTATATGCAATTCAAAATTCATGCGGACCAGAGATGTTTCATTTTATAAGCGAAACTGAAACGGCCAAAATTGCTTGGGAGACTTTGCAAAAAATTTCGACATTAAGAGGGCGTGTTGAGAACAGTGCCGAAAACTTCGAGCCTTACATACCTTTCACCAAATTTGTGAGCGAAGGTGATTGGAGTAAGGCAAAGGAGTACCTTAAACTTAAAGAACTAGATCTCCACAGCGCCGTAAGAGCAATAGATCCGAGAATCGGGTTTGGAGACACAGCCATTCACTTAGCAGCCAGGCAAGGACACGTGAATATTGTGAAAGAGTTGGTGCTGTTGATGACCCCAGAAGATTTGAAAATGAAAAACGCTAAAGGTGCAACAGCTCTTCATGCAGCAGCATGGAGTGGGCAATTGCTTACTGTCAAGGAGTTGGCGCTGTTGATGGGAGAAGAAATAAACACCGTTGGTGACACAGCTCTTCACACAGCAGTACGTAGAGGGAAGGTGGACATTGTGAAAGAGTTGGTGCTGTTTATTCCAAAAGACTTGAAAATAAAAAATAATTATGGTTACACAGCTCTTCACCTAGCAGTCGAGATGAGAAATGTGCCTATAGTGAAAGATTTGGCAGCGTTGGGGGGAGAAGTGAGAGGCCCTGATGGTGACACTGCTCTTCATTTAGCAGTCAAGATGGGGGCTGCAAAAATTGTGAAAGAGTTGGTGTTGTTAATGAGAAGGGAAGATTTGGCAATAAAAAATGATGAAGGTTACACAGCTTTTCACCTAGCGGTAAAGATGGGTAATGTGCCTGCTGTGAAACAGTTCATGACAAAAGAAAAAGAGCGAGACTTTGATAGCTATATTCAGTTCACCACTCACGTGGCAAATGGTGATTGGGGTAATGCAAAGGAGTGCCTTACAAAACTAGATGATCCCTGTGACGCAATAACGATAGTTGATCCAAGAGACGGAAACACTGTTCTTCATGTAGCAGCTAGAGAAGGTCATGTGCGTATTGTCAAAGAATTGATATCATTAGTGAGACAAGAAAATTTGCAACAGAAAACTGTAGAAGATTTCGCAACTTTTGGTTATGGTATAAATTTAGGCGTCACTGAAGAAGTTCTCATGGAAAAGGTCAAATATATGGTTGAACAGTACGAGAAAACACTTGCCAGTATTTTGGATGTACAAAATGCACAAGGTTCCACAGCCCTTCACATAGCAGTATCCAAGGGTAATTTAGATATTGTGAAAGAGTTGGTACCATTAATGAGAAAAGAAGGTTTAGAAATAGAAGATGTTGAAGGATACACAGCTCTTCACAAAGCAGTTGGGAATGGTTATATTGATATTGTGAGAGAGTTGGTGCCAGTGATGAGACAAGAAGGGTTAGAATTAAAAACAGCTGGGGGCTTCAATGCACTTCACCTTGCAGTCATGTCTGAGCTGACTGAGCATATGAATATTGCAAAAGAGGTGATGCCATATATGAGAAAAGAAGCTTTGGAAGAAAAAGATGATGAAGGTTATACAGCTTTAGGTCGTACGTTAGAAGTAAGCGAGGATAAAGACGTGATGGAAATAGCTGGGTACATGGCTGAAAACAACAATAAAGTATTTGGCATTAGGACGTCTCCTGGTAATTGGATTCCAGTTGTCAGTGCTACCGTTAAGAGAGAGTGGGATTTGTGTCGCTATCTCTATTCTCGTACTCCCCCTGAATATCTAATGCCTGAGCACGGGATGGATGGCGCACGGTTGATTATCGATTGCTTTAGAGCGAAGGAGCTTGGTATGGCTTTGGACTTACTTCGGCGTTGCCCTAGATTGGCCATCAGTCCAACCAACTCTGGATGTCCCCCCATAATGGAATTGGCTGGCATGCATTCTGCATTCTTGAGTGGAACCCAACTTGGATTTTGGCAACATTTGATCTATAATCGTTTACTCATTGAACCTGCGAATTTGGAAACTCCTGATACTTGCATCAATATTTCAAGACAACAGGATGGTGGAGACAATCAAATGGATCTCATTCATTCAGTTATAACTTTATACAGGAAACTTGCAAACAGTATCCTTGAACTTTTGGGAGTCAATCGGTTACGTAAAGTGAAATGGATCCATACTCGAAGCATTGATGTTTTACATTACATTGGGAAAATGATAGCGAATGACAATCCTACATCAGCACAACTTGATATTGTAAGAACATCGATCTTCAAAGCTGTTGAACACGGGCATGTTGAGTTTGTTACTCATATATGTGAAACACATCCTCCGTTCACAAAATCAAAAGAAGAAAACAAAAACATATTTCAATTTGCGGTTGAATGTCGTCAACATAAAATTTATAGCCTTATTCATAGACTTGATAGAGAGGACAAGAGATTCTTTGGAATATCTCCAACCGAGTTTCCAGAGCATATGTTACATTTGGCAGGGAAATTATCTCCACAATCAAGGTTCAATCATATTCGTGGTGCAGCTTTGCAAATGCAACGAGAGCTACAGTGGTTTAAGGAGGTGGAGGATATTGTACCTGAGGAGATGCATGAAGTTCCAAATTCAGATGGTATGACACCACGTCAACTGTTTACCAAGAATCATGAGAACCTCAAAGAGGAGGCAGAAAAGTCGATGAAAGGGATTGCAACTTCTTGTACAGTTGTAGGCACTCTCATTGTGACCATTATGTATGCTGCTGCATTTACAGTTCCTGGTGGAAACAGTGGTAGTACAGGTTACCCGATGTTTCTAGCTAAGAGCTTGTTTCTGGTGTTCATAATTTCAGACACTCTATCACTCGTTTCTTCCACAACTTCAGTCATATTATTTTTGGGAATCCTCACCTCACGTTATGCAGCAGATGATTTTCTCAAGGATTTGCCAACAAAGATGATGATAGGCCTTTCCACCCTTTTTCTCTCTATTGCCACCATGATGATTGCCTTTTCTTCTGCCCTCATCATTATGCTTAATAAAGAATACTCATGGGCCATTGTTCCTTGCATTTTTGTTGCGAGTATTCCAGTTGGCTCATTTATATGGTTGCTATTTCCCCTCCTTATTGAGATATTATGGTCAACTTATGGACCAGGAATATTTGACAAGAAAGCCAAACCATGGATTAAACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1114

Amino Acids

125.24

Weight (kDa)

6.16

Isoelectric Point (pI)

35.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 16 - 42 6.9e-09 Domain of unknown function (DUF4219)
Retrotran_gag_2 PF14223 29 - 100 8.2e-09 gag-polypeptide of LTR copia-type
Ank_2 PF12796 148 - 220 4.6e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 209 - 279 5.6e-11 Ankyrin repeats (3 copies)
Ank_5 PF13857 284 - 330 3.8e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 286 - 344 8.1e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 462 - 524 1.2e-12 Ankyrin repeats (3 copies)
PGG PF13962 947 - 1059 7.4e-23 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 251, 2252
AarI CACCTGC 1 cut(s) 5
AatII GACGTC 1 cut(s) 1838
Acc36I ACCTGC 2 cut(s) 5, 2170
Acc65I GGTACC 1 cut(s) 1465
AccB1I GGYRCC 2 cut(s) 1465, 1570
AccB7I CCANNNNNTGG 1 cut(s) 3330
AciI CCGC 5 cut(s) 12, 79, 228, 989, 2531
AclWI GGATC 6 cut(s) 446, 1127, 1154, 2241, 2320, 2333
AcoI YGGCCR 3 cut(s) 99, 267, 2034
AcsI RAATTY 9 cut(s) 149, 220, 297, 365, 1252, 1303, 2167, 2553, 2770
AcuI CTGAAG 2 cut(s) 1338, 3012
AcvI CACGTG 2 cut(s) 508, 1081
AcyI GRCGYC 2 cut(s) 1311, 1835
AfiI CCNNNNNNNGG 7 cut(s) 461, 1169, 1943, 2032, 2071, 2827, 3330
AflIII ACRYGT 1 cut(s) 505
AhdI GACNNNNNGTC 1 cut(s) 2790
AjiI CACGTC 2 cut(s) 1777, 2792
AjnI CCWGG 4 cut(s) 494, 1840, 2923, 3301
AleI CACNNNNGTG 1 cut(s) 1079
AloI GAACNNNNNNTCC 4 cut(s) 411, 443, 1164, 1196
Alw21I GWGCWC 1 cut(s) 1944
Alw26I GTCTC 9 cut(s) 25, 278, 465, 1041, 1161, 1237, 1576, 1842, 2214
AlwI GGATC 6 cut(s) 446, 1127, 1154, 2241, 2320, 2333
AlwNI CAGNNNCTG 1 cut(s) 2924
AoxI GGCC 6 cut(s) 99, 267, 853, 2034, 3112, 3201
ApoI RAATTY 9 cut(s) 149, 220, 297, 365, 1252, 1303, 2167, 2553, 2770
AseI ATTAAT 1 cut(s) 1472
Asp700I GAANNNNTTC 2 cut(s) 1022, 1323
Asp718I GGTACC 1 cut(s) 1465
AspLEI GCGC 3 cut(s) 437, 637, 1958
AspS9I GGNCC 4 cut(s) 230, 854, 3201, 3299
AvaII GGWCC 2 cut(s) 230, 3299
AxyI CCTNAGG 1 cut(s) 2748
BalI TGGCCA 1 cut(s) 2036
BamHI GGATCC 1 cut(s) 2325
BanI GGYRCC 2 cut(s) 1465, 1570
BbrPI CACGTG 2 cut(s) 508, 1081
Bbv12I GWGCWC 1 cut(s) 1944
BceAI ACGGC 2 cut(s) 282, 422
BcgI CGANNNNNNTGC 2 cut(s) 2823, 2857
BciT130I CCWGG 4 cut(s) 496, 1842, 2925, 3303
BciVI GTATCC 3 cut(s) 1441, 1505, 2291
BcoDI GTCTC 9 cut(s) 25, 278, 465, 1041, 1161, 1237, 1576, 1842, 2214
BfaI CTAG 7 cut(s) 422, 788, 986, 1127, 1197, 2028, 2963
BfmI CTRYAG 3 cut(s) 813, 1269, 2714
BfoI RGCGCY 2 cut(s) 438, 638
BfuAI ACCTGC 2 cut(s) 5, 2170
BfuI GTATCC 3 cut(s) 1441, 1505, 2291
BglII AGATCT 1 cut(s) 424
Bme1390I CCNGG 4 cut(s) 496, 1842, 2925, 3303
Bme18I GGWCC 2 cut(s) 230, 3299
BmeRI GACNNNNNGTC 1 cut(s) 2790
BmgBI CACGTC 2 cut(s) 1777, 2792
BmgT120I GGNCC 4 cut(s) 230, 854, 3201, 3299
BmiI GGNNCC 6 cut(s) 138, 1408, 1467, 1572, 2110, 2327
BmrFI CCNGG 4 cut(s) 496, 1842, 2925, 3303
BmsI GCATC 3 cut(s) 1674, 2199, 2745
Bpu10I CCTNAGC 2 cut(s) 198, 1938
BpuEI CTTGAG 2 cut(s) 2122, 3068
Bsa29I ATCGAT 2 cut(s) 1971, 2420
BsaAI YACGTR 4 cut(s) 508, 695, 1081, 2312
BsaHI GRCGYC 2 cut(s) 1311, 1835
BsaJI CCNNGG 2 cut(s) 1434, 3329
BsaXI ACNNNNNCTCC 4 cut(s) 411, 441, 2467, 2497
Bsc4I CCNNNNNNNGG 7 cut(s) 461, 1169, 1943, 2032, 2071, 2827, 3330
Bse1I ACTGG 4 cut(s) 1377, 1574, 1856, 3236
Bse21I CCTNAGG 1 cut(s) 2748
BseBI CCWGG 4 cut(s) 496, 1842, 2925, 3303
BseCI ATCGAT 2 cut(s) 1971, 2420
BseDI CCNNGG 2 cut(s) 1434, 3329
BseGI GGATG 6 cut(s) 1393, 1953, 1957, 2063, 2218, 2479
BseLI CCNNNNNNNGG 7 cut(s) 461, 1169, 1943, 2032, 2071, 2827, 3330
BseMII CTCAG 4 cut(s) 1638, 1647, 1929, 2739
BseNI ACTGG 4 cut(s) 1377, 1574, 1856, 3236
BseRI GAGGAG 2 cut(s) 2765, 2843
BseYI CCCAGC 2 cut(s) 1610, 1790
BsgI GTGCAG 1 cut(s) 2709
BshFI GGCC 6 cut(s) 101, 269, 855, 2036, 3114, 3203
BshNI GGYRCC 2 cut(s) 1465, 1570
BshVI ATCGAT 2 cut(s) 1971, 2420
BsiHKAI GWGCWC 1 cut(s) 1944
BsiWI CGTACG 1 cut(s) 1748
BslFI GGGAC 1 cut(s) 2046
BslI CCNNNNNNNGG 7 cut(s) 461, 1169, 1943, 2032, 2071, 2827, 3330
BsmAI GTCTC 9 cut(s) 25, 278, 465, 1041, 1161, 1237, 1576, 1842, 2214
BsmBI CGTCTC 2 cut(s) 1161, 1842
BsmFI GGGAC 1 cut(s) 2046
BsmI GAATGC 3 cut(s) 199, 2089, 2096
BsnI GGCC 6 cut(s) 101, 269, 855, 2036, 3114, 3203
Bsp1286I GDGCHC 1 cut(s) 1944
Bsp1407I TGTACA 2 cut(s) 1390, 2867
Bsp143I GATC 8 cut(s) 424, 451, 1132, 1159, 2136, 2233, 2325, 2421
Bsp19I CCATGG 1 cut(s) 3329
BspACI CCGC 5 cut(s) 12, 79, 228, 989, 2531
BspANI GGCC 6 cut(s) 101, 269, 855, 2036, 3114, 3203
BspCNI CTCAG 4 cut(s) 1639, 1648, 1930, 2740
BspDI ATCGAT 2 cut(s) 1971, 2420
BspHI TCATGA 2 cut(s) 1026, 2812
BspLI GGNNCC 6 cut(s) 138, 1408, 1467, 1572, 2110, 2327
BspMI ACCTGC 2 cut(s) 5, 2170
BspPI GGATC 6 cut(s) 446, 1127, 1154, 2241, 2320, 2333
BspQI GCTCTTC 5 cut(s) 591, 684, 786, 879, 1527
BspT107I GGYRCC 2 cut(s) 1465, 1570
BsrGI TGTACA 2 cut(s) 1390, 2867
BsrI ACTGG 4 cut(s) 1377, 1574, 1856, 3236
BssECI CCNNGG 2 cut(s) 1434, 3329
BssMI GATC 8 cut(s) 424, 451, 1132, 1159, 2136, 2233, 2325, 2421
BssNI GRCGYC 2 cut(s) 1311, 1835
BssT1I CCWWGG 2 cut(s) 1434, 3329
Bst2UI CCWGG 4 cut(s) 496, 1842, 2925, 3303
Bst6I CTCTTC 5 cut(s) 591, 684, 786, 879, 1527
BstACI GRCGYC 2 cut(s) 1311, 1835
BstAUI TGTACA 2 cut(s) 1390, 2867
BstBAI YACGTR 4 cut(s) 508, 695, 1081, 2312
BstC8I GCNNGC 3 cut(s) 1011, 2083, 2087
BstDEI CTNAG 7 cut(s) 198, 487, 1647, 1656, 1938, 2748, 2967
BstDSI CCRYGG 1 cut(s) 3329
BstEII GGTNACC 1 cut(s) 2948
BstENI CCTNNNNNAGG 1 cut(s) 2825
BstF5I GGATG 6 cut(s) 1393, 1953, 1957, 2063, 2218, 2479
BstH2I RGCGCY 2 cut(s) 438, 638
BstHHI GCGC 3 cut(s) 437, 637, 1958
BstKTI GATC 8 cut(s) 427, 454, 1135, 1162, 2139, 2236, 2328, 2424
BstMAI GTCTC 9 cut(s) 25, 278, 465, 1041, 1161, 1237, 1576, 1842, 2214
BstMBI GATC 8 cut(s) 424, 451, 1132, 1159, 2136, 2233, 2325, 2421
BstMWI GCNNNNNNNGC 7 cut(s) 209, 275, 443, 575, 590, 1657, 2710
BstNI CCWGG 4 cut(s) 496, 1842, 2925, 3303
BstNSI RCATGY 2 cut(s) 2089, 2450
BstPI GGTNACC 1 cut(s) 2948
BstSCI CCNGG 4 cut(s) 494, 1840, 2923, 3301
BstSFI CTRYAG 3 cut(s) 813, 1269, 2714
BstSNI TACGTA 2 cut(s) 695, 2312
BstX2I RGATCY 4 cut(s) 424, 451, 2233, 2325
BstXI CCANNNNNNTGG 2 cut(s) 1081, 1384
BstYI RGATCY 4 cut(s) 424, 451, 2233, 2325
Bsu15I ATCGAT 2 cut(s) 1971, 2420
Bsu36I CCTNAGG 1 cut(s) 2748
BsuI GTATCC 3 cut(s) 1441, 1505, 2291
BsuRI GGCC 6 cut(s) 101, 269, 855, 2036, 3114, 3203
BsuTUI ATCGAT 2 cut(s) 1971, 2420
BtgI CCRYGG 1 cut(s) 3329
BtrI CACGTC 2 cut(s) 1777, 2792
BtsCI GGATG 6 cut(s) 1393, 1953, 1957, 2063, 2218, 2479
BtsI GCAGTG 1 cut(s) 867
BtsIMutI CAGTG 8 cut(s) 334, 867, 1176, 1314, 1581, 1870, 2723, 2941
BveI ACCTGC 2 cut(s) 5, 2170
Cac8I GCNNGC 3 cut(s) 1011, 2083, 2087
CaiI CAGNNNCTG 1 cut(s) 2924
CciI TCATGA 2 cut(s) 1026, 2812
CfoI GCGC 3 cut(s) 437, 637, 1958
Cfr13I GGNCC 4 cut(s) 230, 854, 3201, 3299
ClaI ATCGAT 2 cut(s) 1971, 2420
CseI GACGC 2 cut(s) 1151, 1300
DdeI CTNAG 7 cut(s) 198, 487, 1647, 1656, 1938, 2748, 2967
DpnI GATC 8 cut(s) 426, 453, 1134, 1161, 2138, 2235, 2327, 2423
DpnII GATC 8 cut(s) 424, 451, 1132, 1159, 2136, 2233, 2325, 2421
DriI GACNNNNNGTC 1 cut(s) 2790
EaeI YGGCCR 3 cut(s) 99, 267, 2034
Eam1104I CTCTTC 5 cut(s) 591, 684, 786, 879, 1527
Eam1105I GACNNNNNGTC 1 cut(s) 2790
EarI CTCTTC 5 cut(s) 591, 684, 786, 879, 1527
Eco105I TACGTA 2 cut(s) 695, 2312
Eco130I CCWWGG 2 cut(s) 1434, 3329
Eco147I AGGCCT 1 cut(s) 3114
Eco32I GATATC 1 cut(s) 1233
Eco47I GGWCC 2 cut(s) 230, 3299
Eco57I CTGAAG 2 cut(s) 1338, 3012
Eco72I CACGTG 2 cut(s) 508, 1081
Eco81I CCTNAGG 1 cut(s) 2748
Eco91I GGTNACC 1 cut(s) 2948
EcoNI CCTNNNNNAGG 1 cut(s) 2825
EcoO109I RGGNCCY 1 cut(s) 854
EcoO65I GGTNACC 1 cut(s) 2948
EcoRII CCWGG 4 cut(s) 494, 1840, 2923, 3301
EcoRV GATATC 1 cut(s) 1233
EcoT14I CCWWGG 2 cut(s) 1434, 3329
EcoT22I ATGCAT 2 cut(s) 2091, 2760
ErhI CCWWGG 2 cut(s) 1434, 3329
Esp3I CGTCTC 2 cut(s) 1161, 1842
FalI AAGNNNNNCTT 2 cut(s) 2582, 2614
FaqI GGGAC 1 cut(s) 2046
FauI CCCGC 1 cut(s) 72
FauNDI CATATG 2 cut(s) 1662, 2631
FokI GGATG 6 cut(s) 1400, 1960, 1964, 2070, 2225, 2466
FspBI CTAG 7 cut(s) 422, 788, 986, 1127, 1197, 2028, 2963
GlaI GCGC 3 cut(s) 436, 636, 1957
GsaI CCCAGC 2 cut(s) 1614, 1794
HaeII RGCGCY 2 cut(s) 438, 638
HaeIII GGCC 6 cut(s) 101, 269, 855, 2036, 3114, 3203
HgaI GACGC 2 cut(s) 1151, 1300
HhaI GCGC 3 cut(s) 437, 637, 1958
Hin1I GRCGYC 2 cut(s) 1311, 1835
Hin6I GCGC 3 cut(s) 435, 635, 1956
HinP1I GCGC 3 cut(s) 435, 635, 1956
HincII GTYRAC 3 cut(s) 2546, 2795, 3291
HindII GTYRAC 3 cut(s) 2546, 2795, 3291
HindIII AAGCTT 1 cut(s) 1704
HinfI GANTC 6 cut(s) 459, 1852, 2298, 2595, 2809, 3048
Hpy166II GTNNAC 8 cut(s) 709, 1071, 2150, 2490, 2546, 2795, 2802, 3291
Hpy188I TCNGA 5 cut(s) 456, 1648, 2776, 2998, 3344
Hpy188III TCNNGA 9 cut(s) 796, 889, 1027, 2055, 2101, 2180, 2202, 2624, 2813
Hpy8I GTNNAC 8 cut(s) 709, 1071, 2150, 2490, 2546, 2795, 2802, 3291
HpyCH4IV ACGT 9 cut(s) 507, 694, 1080, 1751, 1776, 1835, 2311, 2791, 3061
HpyF10VI GCNNNNNNNGC 7 cut(s) 209, 275, 443, 575, 590, 1657, 2710
HpyF3I CTNAG 7 cut(s) 198, 487, 1647, 1656, 1938, 2748, 2967
HpySE526I ACGT 9 cut(s) 507, 694, 1080, 1751, 1776, 1835, 2311, 2791, 3061
Hsp92I GRCGYC 2 cut(s) 1311, 1835
HspAI GCGC 3 cut(s) 435, 635, 1956
KpnI GGTACC 1 cut(s) 1469
Kzo9I GATC 8 cut(s) 424, 451, 1132, 1159, 2136, 2233, 2325, 2421
LguI GCTCTTC 5 cut(s) 591, 684, 786, 879, 1527
LmnI GCTCC 1 cut(s) 1990
LweI GCATC 3 cut(s) 1674, 2199, 2745
MaeI CTAG 7 cut(s) 422, 788, 986, 1127, 1197, 2028, 2963
MaeII ACGT 9 cut(s) 507, 694, 1080, 1751, 1776, 1835, 2311, 2791, 3061
MalI GATC 8 cut(s) 426, 453, 1134, 1161, 2138, 2235, 2327, 2423
MboI GATC 8 cut(s) 424, 451, 1132, 1159, 2136, 2233, 2325, 2421
MfeI CAATTG 1 cut(s) 611
MflI RGATCY 4 cut(s) 424, 451, 2233, 2325
MhlI GDGCHC 1 cut(s) 1944
MlsI TGGCCA 1 cut(s) 2036
MluNI TGGCCA 1 cut(s) 2036
MlyI GAGTC 1 cut(s) 2307
MmeI TCCRAC 2 cut(s) 2069, 2636
Mox20I TGGCCA 1 cut(s) 2036
Mph1103I ATGCAT 2 cut(s) 2091, 2760
MroXI GAANNNNTTC 2 cut(s) 1022, 1323
MscI TGGCCA 1 cut(s) 2036
MslI CAYNNNNRTG 7 cut(s) 1079, 2445, 2469, 2682, 3146, 3176, 3250
Msp20I TGGCCA 1 cut(s) 2036
MspA1I CMGCKG 1 cut(s) 1610
MspR9I CCNGG 4 cut(s) 496, 1842, 2925, 3303
MunI CAATTG 1 cut(s) 611
Mva1269I GAATGC 3 cut(s) 199, 2089, 2096
MvaI CCWGG 4 cut(s) 496, 1842, 2925, 3303
MwoI GCNNNNNNNGC 7 cut(s) 209, 275, 443, 575, 590, 1657, 2710
NcoI CCATGG 1 cut(s) 3329
NdeI CATATG 2 cut(s) 1662, 2631
NdeII GATC 8 cut(s) 424, 451, 1132, 1159, 2136, 2233, 2325, 2421
NlaIV GGNNCC 6 cut(s) 138, 1408, 1467, 1572, 2110, 2327
NmuCI GTSAC 5 cut(s) 668, 863, 1139, 1312, 2890
NsiI ATGCAT 2 cut(s) 2091, 2760
NspI RCATGY 2 cut(s) 2089, 2450
OliI CACNNNNGTG 1 cut(s) 1079
PaeI GCATGC 1 cut(s) 2089
PagI TCATGA 2 cut(s) 1026, 2812
PaqCI CACCTGC 1 cut(s) 5
PceI AGGCCT 1 cut(s) 3114
PciSI GCTCTTC 5 cut(s) 591, 684, 786, 879, 1527
PctI GAATGC 3 cut(s) 199, 2089, 2096
PdmI GAANNNNTTC 2 cut(s) 1022, 1323
PfeI GAWTC 5 cut(s) 459, 1852, 2595, 2809, 3048
Pfl23II CGTACG 1 cut(s) 1748
PflMI CCANNNNNTGG 1 cut(s) 3330
PleI GAGTC 1 cut(s) 2306
PmaCI CACGTG 2 cut(s) 508, 1081
PmlI CACGTG 2 cut(s) 508, 1081
PpsI GAGTC 1 cut(s) 2306
Ppu21I YACGTR 4 cut(s) 508, 695, 1081, 2312
PshBI ATTAAT 1 cut(s) 1472
PsiI TTATAA 2 cut(s) 251, 2252
Psp6I CCWGG 4 cut(s) 494, 1840, 2923, 3301
PspCI CACGTG 2 cut(s) 508, 1081
PspEI GGTNACC 1 cut(s) 2948
PspFI CCCAGC 2 cut(s) 1610, 1790
PspGI CCWGG 4 cut(s) 494, 1840, 2923, 3301
PspLI CGTACG 1 cut(s) 1748
PspN4I GGNNCC 6 cut(s) 138, 1408, 1467, 1572, 2110, 2327
PspPI GGNCC 4 cut(s) 230, 854, 3201, 3299
PstNI CAGNNNCTG 1 cut(s) 2924
PsuI RGATCY 4 cut(s) 424, 451, 2233, 2325
PvuII CAGCTG 1 cut(s) 1610
RseI CAYNNNNRTG 7 cut(s) 1079, 2445, 2469, 2682, 3146, 3176, 3250
SapI GCTCTTC 5 cut(s) 591, 684, 786, 879, 1527
Sau3AI GATC 8 cut(s) 424, 451, 1132, 1159, 2136, 2233, 2325, 2421
Sau96I GGNCC 4 cut(s) 230, 854, 3201, 3299
SchI GAGTC 1 cut(s) 2307
ScrFI CCNGG 4 cut(s) 496, 1842, 2925, 3303
SduI GDGCHC 1 cut(s) 1944
SfaNI GCATC 3 cut(s) 1674, 2199, 2745
SfcI CTRYAG 3 cut(s) 813, 1269, 2714
SinI GGWCC 2 cut(s) 230, 3299
SmiMI CAYNNNNRTG 7 cut(s) 1079, 2445, 2469, 2682, 3146, 3176, 3250
SmlI CTYRAG 2 cut(s) 2101, 3083
SmoI CTYRAG 2 cut(s) 2101, 3083
SnaBI TACGTA 2 cut(s) 695, 2312
SphI GCATGC 1 cut(s) 2089
SseBI AGGCCT 1 cut(s) 3114
SsiI CCGC 5 cut(s) 12, 79, 228, 989, 2531
SspI AATATT 4 cut(s) 514, 1669, 2197, 3309
SspMI CTAG 7 cut(s) 422, 788, 986, 1127, 1197, 2028, 2963
StuI AGGCCT 1 cut(s) 3114
StyD4I CCNGG 4 cut(s) 494, 1840, 2923, 3301
StyI CCWWGG 2 cut(s) 1434, 3329
TaiI ACGT 9 cut(s) 510, 697, 1083, 1754, 1779, 1838, 2314, 2794, 3064
TaqI TCGA 8 cut(s) 123, 302, 342, 795, 1971, 2335, 2420, 2843
TatI WGTACW 3 cut(s) 1390, 2867, 2942
TfiI GAWTC 5 cut(s) 459, 1852, 2595, 2809, 3048
TscAI CASTG 8 cut(s) 334, 874, 1183, 1321, 1581, 1870, 2723, 2941
TseFI GTSAC 5 cut(s) 668, 863, 1139, 1312, 2890
Tsp45I GTSAC 5 cut(s) 668, 863, 1139, 1312, 2890
TspGWI ACGGA 2 cut(s) 1185, 2475
TspRI CASTG 8 cut(s) 334, 874, 1183, 1321, 1581, 1870, 2723, 2941
Van91I CCANNNNNTGG 1 cut(s) 3330
VpaK11BI GGWCC 2 cut(s) 230, 3299
VspI ATTAAT 1 cut(s) 1472
XagI CCTNNNNNAGG 1 cut(s) 2825
XapI RAATTY 9 cut(s) 149, 220, 297, 365, 1252, 1303, 2167, 2553, 2770
XceI RCATGY 2 cut(s) 2089, 2450
XcmI CCANNNNNNNNNTGG 1 cut(s) 2074
XmnI GAANNNNTTC 2 cut(s) 1022, 1323
XspI CTAG 7 cut(s) 422, 788, 986, 1127, 1197, 2028, 2963
ZraI GACGTC 1 cut(s) 1836
Zsp2I ATGCAT 2 cut(s) 2091, 2760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.