RchiOBHm_Chr6g0257321

Belongs to the glycosyl hydrolase 13 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0017595
Physical Location & Seq
Reverse (-)
12613043 .. 12614488
1446 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 528 bp
ATGAATTCACTAAAAATTTCCCTCCTCTGTTTCCTACTCTCTATCATTTTCATACCCAACTTCACTGCTTCCCAAATCCTATTTCAGGGGTTCAATTGGGAATCGGCAAAGAAGGGAGGATGGTACAACATCCTGAAACAGTCTGCAGCTGAACTAGCTTCTTCAGGAATTACACATGTTTGGCTTCCACCACCTTCTCATGCTATTTCAGATGAAGGATACATGCCTGGCAGGCTGTATGATCTCAATGTATCTAAATATGGAAATCAAGATGATTTAAAAGCACTGATAAAAGCCTACCGTGACAGTGGGATACAGAGCATAGCAGATATTGTTATAAACCACAGATGTGCAGAGAAGCAAGATGAGAGAGGAATATGGTGCATTTTTGAAGGAGGAACACCTGATGATCGCCTTGATTGGGGGCCGTCTCTTATTTGCAGTGATGACACACAATATTCTGATGGCAAGGGCAATCCTGACACTGGAGCTGACTTTGGGGGTGCTCCTGACAAGGGTCCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko04973 map00500 map01100 map04973
Pfam Domains
Protein Families
Alpha-amyl_C2 Alpha-amylase

Protein Analysis

175

Amino Acids

19.22

Weight (kDa)

4.78

Isoelectric Point (pI)

25.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alpha-amylase PF00128 54 - 120 1.1e-07 Alpha amylase, catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06030
malus_domestica MD00G1215700.v1.1 MD15G1083100.v1.1
prunus_persica Prupe.8G125300_v2.0.a1
pyrus_communis pycom05g07400 pycom05g07450
rosa_chinensis RchiOBHm_Chr6g0257211 RchiOBHm_Chr6g0257321
rosa_rugosa Rorug05G0574100
rosa_samantha Rh6AG092000 Rh6CG079700 Rh6DG075200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 338
AcsI RAATTY 2 cut(s) 4, 15
AcuI CTGAAG 1 cut(s) 147
AfaI GTAC 1 cut(s) 125
AfiI CCNNNNNNNGG 4 cut(s) 85, 421, 485, 515
AflIII ACRYGT 1 cut(s) 175
AgsI TTSAA 2 cut(s) 94, 392
AjnI CCWGG 1 cut(s) 226
AjuI GAANNNNNNNTTGG 2 cut(s) 66, 98
AleI CACNNNNGTG 1 cut(s) 348
AluBI AGCT 3 cut(s) 149, 158, 491
AluI AGCT 3 cut(s) 149, 158, 491
Alw21I GWGCWC 1 cut(s) 508
Alw26I GTCTC 1 cut(s) 435
AoxI GGCC 1 cut(s) 425
ApeKI GCWGC 1 cut(s) 146
ApoI RAATTY 2 cut(s) 4, 15
AspS9I GGNCC 2 cut(s) 425, 518
AvaII GGWCC 1 cut(s) 518
Bbv12I GWGCWC 1 cut(s) 508
BbvI GCAGC 1 cut(s) 158
BccI CCATC 2 cut(s) 114, 458
BceAI ACGGC 1 cut(s) 412
BciT130I CCWGG 1 cut(s) 228
BciVI GTATCC 2 cut(s) 212, 306
BcoDI GTCTC 1 cut(s) 435
BfaI CTAG 1 cut(s) 155
BfmI CTRYAG 1 cut(s) 144
BfuI GTATCC 2 cut(s) 212, 306
BglI GCCNNNNNGGC 1 cut(s) 232
BisI GCNGC 1 cut(s) 147
BlsI GCNGC 1 cut(s) 148
Bme1390I CCNGG 1 cut(s) 228
Bme18I GGWCC 1 cut(s) 518
BmgT120I GGNCC 2 cut(s) 425, 518
BmiI GGNNCC 2 cut(s) 426, 519
BmrFI CCNGG 1 cut(s) 228
BoxI GACNNNNGTC 1 cut(s) 516
BpmI CTGGAG 1 cut(s) 507
Bsc4I CCNNNNNNNGG 4 cut(s) 85, 421, 485, 515
Bse1I ACTGG 1 cut(s) 490
BseBI CCWGG 1 cut(s) 228
BseGI GGATG 2 cut(s) 125, 129
BseLI CCNNNNNNNGG 4 cut(s) 85, 421, 485, 515
BseNI ACTGG 1 cut(s) 490
BseRI GAGGAG 1 cut(s) 14
BseXI GCAGC 1 cut(s) 158
BsgI GTGCAG 1 cut(s) 372
BshFI GGCC 1 cut(s) 427
BsiHKAI GWGCWC 1 cut(s) 508
BslI CCNNNNNNNGG 4 cut(s) 85, 421, 485, 515
BsmAI GTCTC 1 cut(s) 435
BsmBI CGTCTC 1 cut(s) 435
BsnI GGCC 1 cut(s) 427
Bsp1286I GDGCHC 1 cut(s) 508
Bsp143I GATC 2 cut(s) 241, 409
BspANI GGCC 1 cut(s) 427
BspLI GGNNCC 2 cut(s) 426, 519
BspMAI CTGCAG 1 cut(s) 148
BsrI ACTGG 1 cut(s) 490
BssMI GATC 2 cut(s) 241, 409
Bst2UI CCWGG 1 cut(s) 228
Bst4CI ACNGT 3 cut(s) 141, 302, 308
BstC8I GCNNGC 1 cut(s) 233
BstENI CCTNNNNNAGG 1 cut(s) 83
BstF5I GGATG 2 cut(s) 125, 129
BstKTI GATC 2 cut(s) 244, 412
BstMAI GTCTC 1 cut(s) 435
BstMBI GATC 2 cut(s) 241, 409
BstMWI GCNNNNNNNGC 2 cut(s) 155, 232
BstNI CCWGG 1 cut(s) 228
BstNSI RCATGY 2 cut(s) 179, 226
BstPAI GACNNNNGTC 1 cut(s) 516
BstSCI CCNGG 1 cut(s) 226
BstSFI CTRYAG 1 cut(s) 144
BstV1I GCAGC 1 cut(s) 158
BsuI GTATCC 2 cut(s) 212, 306
BsuRI GGCC 1 cut(s) 427
BtsCI GGATG 2 cut(s) 125, 129
BtsI GCAGTG 2 cut(s) 63, 448
BtsIMutI CAGTG 5 cut(s) 63, 284, 313, 448, 483
Cac8I GCNNGC 1 cut(s) 233
Cfr13I GGNCC 2 cut(s) 425, 518
Csp6I GTAC 1 cut(s) 124
CviAII CATG 3 cut(s) 176, 200, 223
CviJI RGCY 7 cut(s) 149, 158, 184, 235, 296, 427, 491
CviKI_1 RGCY 7 cut(s) 149, 158, 184, 235, 296, 427, 491
CviQI GTAC 1 cut(s) 124
DpnI GATC 2 cut(s) 243, 411
DpnII GATC 2 cut(s) 241, 409
DraI TTTAAA 1 cut(s) 279
Eco47I GGWCC 1 cut(s) 518
Eco57I CTGAAG 1 cut(s) 147
EcoNI CCTNNNNNAGG 1 cut(s) 83
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 1 cut(s) 226
Esp3I CGTCTC 1 cut(s) 435
FaeI CATG 3 cut(s) 179, 203, 226
FaiI YATR 9 cut(s) 53, 177, 201, 224, 240, 261, 323, 338, 379
FatI CATG 3 cut(s) 175, 199, 222
Fnu4HI GCNGC 1 cut(s) 147
FokI GGATG 2 cut(s) 116, 132
Fsp4HI GCNGC 1 cut(s) 147
FspBI CTAG 1 cut(s) 155
GluI GCNGC 1 cut(s) 147
GsuI CTGGAG 1 cut(s) 507
HaeIII GGCC 1 cut(s) 427
Hin1II CATG 3 cut(s) 179, 203, 226
HinfI GANTC 1 cut(s) 101
Hpy188I TCNGA 2 cut(s) 211, 463
Hpy188III TCNNGA 6 cut(s) 133, 165, 269, 479, 509, 521
HpyAV CCTTC 4 cut(s) 106, 204, 209, 386
HpyCH4III ACNGT 3 cut(s) 141, 302, 308
HpyCH4V TGCA 4 cut(s) 146, 353, 384, 441
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 232
Hsp92II CATG 3 cut(s) 179, 203, 226
Kzo9I GATC 2 cut(s) 241, 409
LmnI GCTCC 2 cut(s) 488, 511
Lsp1109I GCAGC 1 cut(s) 158
MaeI CTAG 1 cut(s) 155
MaeIII GTNAC 1 cut(s) 302
MalI GATC 2 cut(s) 243, 411
MboI GATC 2 cut(s) 241, 409
MboII GAAGA 1 cut(s) 153
MfeI CAATTG 1 cut(s) 94
MhlI GDGCHC 1 cut(s) 508
MluCI AATT 4 cut(s) 4, 15, 94, 168
MnlI CCTC 5 cut(s) 32, 35, 110, 365, 389
MseI TTAA 1 cut(s) 278
MslI CAYNNNNRTG 1 cut(s) 348
MspA1I CMGCKG 1 cut(s) 149
MspR9I CCNGG 1 cut(s) 228
MunI CAATTG 1 cut(s) 94
MvaI CCWGG 1 cut(s) 228
MwoI GCNNNNNNNGC 2 cut(s) 155, 232
NdeII GATC 2 cut(s) 241, 409
NlaIII CATG 3 cut(s) 179, 203, 226
NlaIV GGNNCC 2 cut(s) 426, 519
NmuCI GTSAC 1 cut(s) 302
NspI RCATGY 2 cut(s) 179, 226
OliI CACNNNNGTG 1 cut(s) 348
PciI ACATGT 1 cut(s) 175
PfeI GAWTC 1 cut(s) 101
PkrI GCNGC 1 cut(s) 148
PscI ACATGT 1 cut(s) 175
PshAI GACNNNNGTC 1 cut(s) 516
PsiI TTATAA 1 cut(s) 338
Psp6I CCWGG 1 cut(s) 226
PspGI CCWGG 1 cut(s) 226
PspN4I GGNNCC 2 cut(s) 426, 519
PspPI GGNCC 2 cut(s) 425, 518
PstI CTGCAG 1 cut(s) 148
PvuII CAGCTG 1 cut(s) 149
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
RseI CAYNNNNRTG 1 cut(s) 348
SaqAI TTAA 1 cut(s) 278
SatI GCNGC 1 cut(s) 147
Sau3AI GATC 2 cut(s) 241, 409
Sau96I GGNCC 2 cut(s) 425, 518
ScrFI CCNGG 1 cut(s) 228
SduI GDGCHC 1 cut(s) 508
SetI ASST 5 cut(s) 151, 160, 196, 406, 493
SfcI CTRYAG 1 cut(s) 144
SinI GGWCC 1 cut(s) 518
SmiMI CAYNNNNRTG 1 cut(s) 348
Sse9I AATT 4 cut(s) 4, 15, 94, 168
SspI AATATT 1 cut(s) 458
SspMI CTAG 1 cut(s) 155
StyD4I CCNGG 1 cut(s) 226
TaaI ACNGT 3 cut(s) 141, 302, 308
TasI AATT 4 cut(s) 4, 15, 94, 168
TfiI GAWTC 1 cut(s) 101
Tru1I TTAA 1 cut(s) 278
Tru9I TTAA 1 cut(s) 278
TscAI CASTG 5 cut(s) 70, 291, 313, 448, 490
TseFI GTSAC 1 cut(s) 302
TseI GCWGC 1 cut(s) 146
Tsp45I GTSAC 1 cut(s) 302
TspDTI ATGAA 3 cut(s) 17, 40, 228
TspRI CASTG 5 cut(s) 70, 291, 313, 448, 490
VpaK11BI GGWCC 1 cut(s) 518
XagI CCTNNNNNAGG 1 cut(s) 83
XapI RAATTY 2 cut(s) 4, 15
XceI RCATGY 2 cut(s) 179, 226
XspI CTAG 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.