RchiOBHm_Chr6g0259831

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0017347
Physical Location & Seq
Forward (+)
15039156 .. 15039613
458 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 414 bp
ATGCCTGTGAGGGATTGTGTTTCTTGGAACACAGTGATTAGTGGGTGTGCCAAGAATGGGAGGATGGGGGAGGCATTGCGGCTTTTCGAGTCTATGCCTGAGAGGAGTGTTGTGTCTTGGAATGCAATGATTACTGGGTTTTTGCAGAATGGTGATGTAGTGAGTGCAGTTGAGTTTTTTGAGAGAATGCCACAGCTGGATGGAGCTTCTGTTAGTGCACTTGTGTCGGGGCTTATTCAAAACGGCGAGCTGGGTGAAGCTGCGAGGATTGTTGTTCAATGTGGGGATGGGGGTGAGGGTGTGGAAGATTTGGTGCATGCTTATAATACATTGATTGCTGGTTATGGTCAGAGAGGAAGGGTGGAAGAAGCTCGGCGCATTTTTGATCAAAGCCCATTTTGTCATATGATATAA

Protein Analysis

137

Amino Acids

14.88

Weight (kDa)

4.83

Isoelectric Point (pI)

42.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 4 - 32 3.6e-08 PPR repeat
PPR_2 PF13041 5 - 37 9.1e-09 PPR repeat family
PPR PF01535 7 - 36 4.3e-10 PPR repeat
PPR_2 PF13041 36 - 64 8.6e-07 PPR repeat family
PPR PF01535 38 - 65 2.9e-07 PPR repeat
PPR PF01535 107 - 130 4.3e-06 PPR repeat
PPR_1 PF12854 107 - 130 6.1e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 324
AciI CCGC 1 cut(s) 79
AfiI CCNNNNNNNGG 1 cut(s) 57
AgsI TTSAA 2 cut(s) 239, 278
AluBI AGCT 5 cut(s) 196, 206, 250, 260, 371
AluI AGCT 5 cut(s) 196, 206, 250, 260, 371
Alw21I GWGCWC 1 cut(s) 220
Alw44I GTGCAC 1 cut(s) 216
ApaLI GTGCAC 1 cut(s) 216
ApeKI GCWGC 1 cut(s) 260
AspLEI GCGC 1 cut(s) 378
AsuHPI GGTGA 3 cut(s) 164, 266, 305
BaeGI GKGCMC 1 cut(s) 220
Bbv12I GWGCWC 1 cut(s) 220
BbvI GCAGC 1 cut(s) 247
BccI CCATC 3 cut(s) 58, 194, 281
BceAI ACGGC 1 cut(s) 259
BcgI CGANNNNNNTGC 2 cut(s) 207, 241
BclI TGATCA 1 cut(s) 385
BisI GCNGC 2 cut(s) 80, 261
BlsI GCNGC 2 cut(s) 81, 262
BmrI ACTGGG 1 cut(s) 144
BmuI ACTGGG 1 cut(s) 144
BsaXI ACNNNNNCTCC 2 cut(s) 195, 225
Bsc4I CCNNNNNNNGG 1 cut(s) 57
Bse1I ACTGG 1 cut(s) 139
Bse3DI GCAATG 2 cut(s) 74, 132
BseGI GGATG 3 cut(s) 69, 205, 292
BseLI CCNNNNNNNGG 1 cut(s) 57
BseMI GCAATG 2 cut(s) 74, 132
BseMII CTCAG 1 cut(s) 90
BseNI ACTGG 1 cut(s) 139
BseRI GAGGAG 1 cut(s) 118
BseSI GKGCMC 1 cut(s) 220
BseXI GCAGC 1 cut(s) 247
BseYI CCCAGC 1 cut(s) 250
BsgI GTGCAG 1 cut(s) 186
BsiHKAI GWGCWC 1 cut(s) 220
BslI CCNNNNNNNGG 1 cut(s) 57
BsmI GAATGC 2 cut(s) 127, 192
Bsp1286I GDGCHC 1 cut(s) 220
Bsp143I GATC 1 cut(s) 385
BspACI CCGC 1 cut(s) 79
BspCNI CTCAG 1 cut(s) 91
BsrDI GCAATG 2 cut(s) 74, 132
BsrI ACTGG 1 cut(s) 139
BssMI GATC 1 cut(s) 385
Bst4CI ACNGT 1 cut(s) 34
BstC8I GCNNGC 2 cut(s) 248, 318
BstDEI CTNAG 1 cut(s) 99
BstF5I GGATG 3 cut(s) 69, 205, 292
BstHHI GCGC 1 cut(s) 378
BstKTI GATC 1 cut(s) 388
BstMBI GATC 1 cut(s) 385
BstNSI RCATGY 1 cut(s) 320
BstSLI GKGCMC 1 cut(s) 220
BstV1I GCAGC 1 cut(s) 247
BtsCI GGATG 3 cut(s) 69, 205, 292
BtsIMutI CAGTG 1 cut(s) 39
Cac8I GCNNGC 2 cut(s) 248, 318
CfoI GCGC 1 cut(s) 378
CviAII CATG 1 cut(s) 317
CviJI RGCY 8 cut(s) 82, 196, 206, 232, 250, 260, 371, 393
CviKI_1 RGCY 8 cut(s) 82, 196, 206, 232, 250, 260, 371, 393
DdeI CTNAG 1 cut(s) 99
DpnI GATC 1 cut(s) 387
DpnII GATC 1 cut(s) 385
FaeI CATG 1 cut(s) 320
FaiI YATR 7 cut(s) 95, 318, 324, 345, 405, 407, 412
FatI CATG 1 cut(s) 316
FauNDI CATATG 1 cut(s) 405
FbaI TGATCA 1 cut(s) 385
Fnu4HI GCNGC 2 cut(s) 80, 261
FokI GGATG 3 cut(s) 76, 212, 299
Fsp4HI GCNGC 2 cut(s) 80, 261
GlaI GCGC 1 cut(s) 377
GluI GCNGC 2 cut(s) 80, 261
GsaI CCCAGC 1 cut(s) 254
HhaI GCGC 1 cut(s) 378
Hin1II CATG 1 cut(s) 320
Hin6I GCGC 1 cut(s) 376
HinP1I GCGC 1 cut(s) 376
HinfI GANTC 1 cut(s) 89
HphI GGTGA 3 cut(s) 164, 266, 305
Hpy166II GTNNAC 1 cut(s) 218
Hpy188I TCNGA 1 cut(s) 351
Hpy8I GTNNAC 1 cut(s) 218
HpyAV CCTTC 1 cut(s) 351
HpyCH4III ACNGT 1 cut(s) 34
HpyCH4V TGCA 5 cut(s) 125, 145, 167, 218, 316
HpyF3I CTNAG 1 cut(s) 99
Hsp92II CATG 1 cut(s) 320
HspAI GCGC 1 cut(s) 376
Ksp22I TGATCA 1 cut(s) 385
Kzo9I GATC 1 cut(s) 385
LmnI GCTCC 1 cut(s) 203
LpnPI CCDG 6 cut(s) 18, 111, 120, 182, 236, 324
Lsp1109I GCAGC 1 cut(s) 247
MalI GATC 1 cut(s) 387
MboI GATC 1 cut(s) 385
MboII GAAGA 2 cut(s) 317, 377
MhlI GDGCHC 1 cut(s) 220
MlyI GAGTC 1 cut(s) 98
MnlI CCTC 7 cut(s) 3, 54, 64, 96, 258, 289, 347
MspA1I CMGCKG 1 cut(s) 196
Mva1269I GAATGC 2 cut(s) 127, 192
NdeI CATATG 1 cut(s) 405
NdeII GATC 1 cut(s) 385
NlaIII CATG 1 cut(s) 320
NmeAIII GCCGAG 1 cut(s) 352
NspI RCATGY 1 cut(s) 320
PaeI GCATGC 1 cut(s) 320
PctI GAATGC 2 cut(s) 127, 192
PkrI GCNGC 2 cut(s) 81, 262
PleI GAGTC 1 cut(s) 97
PpsI GAGTC 1 cut(s) 97
PsiI TTATAA 1 cut(s) 324
PspFI CCCAGC 1 cut(s) 250
PvuII CAGCTG 1 cut(s) 196
SatI GCNGC 2 cut(s) 80, 261
Sau3AI GATC 1 cut(s) 385
SchI GAGTC 1 cut(s) 98
SduI GDGCHC 1 cut(s) 220
SetI ASST 5 cut(s) 198, 208, 252, 262, 373
SphI GCATGC 1 cut(s) 320
SsiI CCGC 1 cut(s) 79
TaaI ACNGT 1 cut(s) 34
TaqI TCGA 1 cut(s) 87
TauI GCSGC 1 cut(s) 82
TscAI CASTG 1 cut(s) 39
TseI GCWGC 1 cut(s) 260
TspRI CASTG 1 cut(s) 39
VneI GTGCAC 1 cut(s) 216
XceI RCATGY 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.