RchiOBHm_Chr6g0261321

Alpha-trehalose glucohydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0017821
Physical Location & Seq
Reverse (-)
16205893 .. 16208648
2756 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 306 bp
ATGTCAAAGTTCGGTGAATTTCTTATTTGGGAAAAAAGTATTGCTCTTTCAAATTCTAAGGCAGTTGCAATTTCGCAGGGCAAGCCATCAAATTTTGTAGAGCTCTCCATCCCAAGGGACTTTCCAAATGATTGGGATCCGATCCAACACATGATAGCTGAAGGCCTGGCAAGGTCTGGATCAAAAGAAGCAAAGTTAGTGGGAGAAGACATTGCTGTAAGGTGGATCAGAACCAATTATAAAGCCTACAAGAAAACAGGTGTCATGCATGAAAAATATGACGTGGAAGAGTATGGAGAATTTTAG

Protein Analysis

101

Amino Acids

11.53

Weight (kDa)

6.11

Isoelectric Point (pI)

31.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalase PF01204 25 - 99 4.6e-17 Trehalase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 240
AclWI GGATC 5 cut(s) 131, 136, 144, 187, 233
AcsI RAATTY 4 cut(s) 17, 52, 91, 299
AcuI CTGAAG 1 cut(s) 180
AfiI CCNNNNNNNGG 1 cut(s) 114
AgsI TTSAA 1 cut(s) 51
AjiI CACGTC 1 cut(s) 283
AjnI CCWGG 1 cut(s) 165
AluBI AGCT 2 cut(s) 103, 158
AluI AGCT 2 cut(s) 103, 158
Alw21I GWGCWC 1 cut(s) 105
AlwI GGATC 5 cut(s) 131, 136, 144, 187, 233
AoxI GGCC 1 cut(s) 163
ApoI RAATTY 4 cut(s) 17, 52, 91, 299
AsuHPI GGTGA 1 cut(s) 26
BamHI GGATCC 1 cut(s) 136
BanII GRGCYC 1 cut(s) 105
BbsI GAAGAC 1 cut(s) 213
Bbv12I GWGCWC 1 cut(s) 105
BccI CCATC 2 cut(s) 94, 116
BciT130I CCWGG 1 cut(s) 167
Bme1390I CCNGG 1 cut(s) 167
BmgBI CACGTC 1 cut(s) 283
BmiI GGNNCC 1 cut(s) 138
BmrFI CCNGG 1 cut(s) 167
BpiI GAAGAC 1 cut(s) 213
BsaBI GATNNNNATC 1 cut(s) 135
BsaJI CCNNGG 1 cut(s) 113
Bsc4I CCNNNNNNNGG 1 cut(s) 114
Bse3DI GCAATG 1 cut(s) 210
Bse8I GATNNNNATC 1 cut(s) 135
BseBI CCWGG 1 cut(s) 167
BseDI CCNNGG 1 cut(s) 113
BseGI GGATG 1 cut(s) 108
BseJI GATNNNNATC 1 cut(s) 135
BseLI CCNNNNNNNGG 1 cut(s) 114
BseMI GCAATG 1 cut(s) 210
BshFI GGCC 1 cut(s) 165
BsiHKAI GWGCWC 1 cut(s) 105
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 1 cut(s) 114
BsmFI GGGAC 1 cut(s) 131
BsnI GGCC 1 cut(s) 165
Bsp1286I GDGCHC 1 cut(s) 105
Bsp143I GATC 4 cut(s) 136, 141, 179, 225
BspANI GGCC 1 cut(s) 165
BspLI GGNNCC 1 cut(s) 138
BspPI GGATC 5 cut(s) 131, 136, 144, 187, 233
BsrDI GCAATG 1 cut(s) 210
BssECI CCNNGG 1 cut(s) 113
BssMI GATC 4 cut(s) 136, 141, 179, 225
BssT1I CCWWGG 1 cut(s) 113
Bst2UI CCWGG 1 cut(s) 167
Bst6I CTCTTC 1 cut(s) 282
BstC8I GCNNGC 1 cut(s) 83
BstDEI CTNAG 1 cut(s) 57
BstF5I GGATG 1 cut(s) 108
BstKTI GATC 4 cut(s) 139, 144, 182, 228
BstMBI GATC 4 cut(s) 136, 141, 179, 225
BstMWI GCNNNNNNNGC 1 cut(s) 82
BstNI CCWGG 1 cut(s) 167
BstSCI CCNGG 1 cut(s) 165
BstV2I GAAGAC 1 cut(s) 213
BstX2I RGATCY 1 cut(s) 136
BstXI CCANNNNNNTGG 1 cut(s) 132
BstYI RGATCY 1 cut(s) 136
BsuRI GGCC 1 cut(s) 165
BtrI CACGTC 1 cut(s) 283
BtsCI GGATG 1 cut(s) 108
Cac8I GCNNGC 1 cut(s) 83
CspCI CAANNNNNGTGG 2 cut(s) 180, 215
CviAII CATG 3 cut(s) 151, 265, 269
CviJI RGCY 5 cut(s) 85, 103, 158, 165, 245
CviKI_1 RGCY 5 cut(s) 85, 103, 158, 165, 245
DdeI CTNAG 1 cut(s) 57
DpnI GATC 4 cut(s) 138, 143, 181, 227
DpnII GATC 4 cut(s) 136, 141, 179, 225
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
Ecl136II GAGCTC 1 cut(s) 103
Eco130I CCWWGG 1 cut(s) 113
Eco147I AGGCCT 1 cut(s) 165
Eco24I GRGCYC 1 cut(s) 105
Eco53kI GAGCTC 1 cut(s) 103
Eco57I CTGAAG 1 cut(s) 180
EcoICRI GAGCTC 1 cut(s) 103
EcoRII CCWGG 1 cut(s) 165
EcoT14I CCWWGG 1 cut(s) 113
EcoT22I ATGCAT 1 cut(s) 270
EcoT38I GRGCYC 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 113
FaeI CATG 3 cut(s) 154, 268, 272
FaiI YATR 6 cut(s) 152, 240, 266, 270, 279, 294
FaqI GGGAC 1 cut(s) 131
FatI CATG 3 cut(s) 150, 264, 268
FokI GGATG 1 cut(s) 95
FriOI GRGCYC 1 cut(s) 105
HaeIII GGCC 1 cut(s) 165
Hin1II CATG 3 cut(s) 154, 268, 272
HphI GGTGA 1 cut(s) 26
Hpy188I TCNGA 2 cut(s) 141, 230
Hpy188III TCNNGA 1 cut(s) 177
HpyAV CCTTC 1 cut(s) 155
HpyCH4IV ACGT 1 cut(s) 282
HpyCH4V TGCA 2 cut(s) 68, 268
HpyF10VI GCNNNNNNNGC 1 cut(s) 82
HpyF3I CTNAG 1 cut(s) 57
HpySE526I ACGT 1 cut(s) 282
Hsp92II CATG 3 cut(s) 154, 268, 272
Kzo9I GATC 4 cut(s) 136, 141, 179, 225
LpnPI CCDG 5 cut(s) 62, 152, 162, 179, 243
MaeII ACGT 1 cut(s) 282
MalI GATC 4 cut(s) 138, 143, 181, 227
MboI GATC 4 cut(s) 136, 141, 179, 225
MboII GAAGA 2 cut(s) 218, 299
MflI RGATCY 1 cut(s) 136
MhlI GDGCHC 1 cut(s) 105
MluCI AATT 6 cut(s) 17, 52, 69, 91, 235, 299
MmeI TCCRAC 1 cut(s) 169
Mph1103I ATGCAT 1 cut(s) 270
MspR9I CCNGG 1 cut(s) 167
MvaI CCWGG 1 cut(s) 167
MwoI GCNNNNNNNGC 1 cut(s) 82
NdeII GATC 4 cut(s) 136, 141, 179, 225
NlaIII CATG 3 cut(s) 154, 268, 272
NlaIV GGNNCC 1 cut(s) 138
NsiI ATGCAT 1 cut(s) 270
PceI AGGCCT 1 cut(s) 165
PsiI TTATAA 1 cut(s) 240
Psp124BI GAGCTC 1 cut(s) 105
Psp6I CCWGG 1 cut(s) 165
PspGI CCWGG 1 cut(s) 165
PspN4I GGNNCC 1 cut(s) 138
PsuI RGATCY 1 cut(s) 136
SacI GAGCTC 1 cut(s) 105
Sau3AI GATC 4 cut(s) 136, 141, 179, 225
ScrFI CCNGG 1 cut(s) 167
SduI GDGCHC 1 cut(s) 105
SetI ASST 6 cut(s) 105, 160, 176, 224, 262, 285
Sse9I AATT 6 cut(s) 17, 52, 69, 91, 235, 299
SseBI AGGCCT 1 cut(s) 165
SstI GAGCTC 1 cut(s) 105
StuI AGGCCT 1 cut(s) 165
StyD4I CCNGG 1 cut(s) 165
StyI CCWWGG 1 cut(s) 113
TaiI ACGT 1 cut(s) 285
TasI AATT 6 cut(s) 17, 52, 69, 91, 235, 299
TspDTI ATGAA 1 cut(s) 285
XapI RAATTY 4 cut(s) 17, 52, 91, 299
Zsp2I ATGCAT 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.