Rroxscaffold_177G00434120

Alpha-trehalose glucohydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000177
OG0017821
Physical Location & Seq
Reverse (-)
43857 .. 52830
8974 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_177G00434120.1

Sequence Viewer

Length: 1062 bp
ATGCCACCCAATGAAGACGTTTCTATGAAGCTAATTCGTGCAGAGAACCTCACCATGGGACTCCAAGGTTTGAAAAAATGGCCTAAACCTCCTGCATTTATACAGGTCTTGGCCTACTGTCTTGCTGCAATAACACCTGTGTCAGATATTCACATACAATACAAAGTCCTGGGATGTCAAAGTTCGGTGAATTTTCTTATTTGGGAAAAAAGTATTGCTCTTTCGAATTATAAGGCAGCTGCAATTTCGCAGGGCAAGCCATCAAATTATGTAGAGCTCTCCATCCCAAGGGACTTTCCAAATGATTGGGCTCCAATCCAACACATGATAGCTGAAGGCCTGGCAAGGTCTGGATCAAAAGAAGCAAAGTTAGTGCGAGAAGACATTGCTGTAAGATGGATCAGAACCAATTATAAAGCCTACAAGAAAACAGATGTCATTCATGAAAAATATGACTGGAAGAGTGTGGAGAATTTTGGAGGTGGTGGTGAATACGTACCCCAGATTTGTCAGCTGGCTGAACAGATTCCAGATGCAATTTTTCTTAAGGTTAACTATGAAGAGCTCAACGCTATGTGCCATGGTCTCCACATTCATGTGATACCATTCTTTAGGTTTTATAGAGGCGCAGAAGGGCGAGTATGTAGCTTCAGCTGCACCATTGCCGCTGTAAGTATTGCAATTCCTGTTGATGTTGGTGCTAATGCAGTTGAGTTCATCATCAACCATGCTGAAGTTCCAATAGCTTTTGTTCAAGAGAACAAAATCCCTACTGGAAGTTCAGATTATGAACTACCTCTAAAACAGAGGACTGCTGTTTGCACAAAAATGTGTAGAAGTGGAACGACTAGAGAACCAAAAGGCGTAATCGTTACTAATACAGCATTAATGTCAGAAGTATTGTCAACAGACCATATACTTTTCCTAAAAGATAGAATGGAGCGGGTTGATAAACTTTATAATGAAGCAAAGGGTGGAAAATGTGTATACAACTACAGTGTCTCCTTCAGGATCGAGAATGGACCTTGTGCCTTACCGCGTTTGTCTAAGAAAATCCGGTAA

Protein Analysis

353

Amino Acids

39.56

Weight (kDa)

8.78

Isoelectric Point (pI)

34.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalase PF01204 98 - 168 2.9e-18 Trehalase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 231, 414, 960
AccBSI CCGCTC 1 cut(s) 943
AccI GTMKAC 1 cut(s) 987
AccII CGCG 1 cut(s) 1039
AciI CCGC 3 cut(s) 666, 943, 1037
AclWI GGATC 3 cut(s) 361, 407, 1019
AcsI RAATTY 2 cut(s) 190, 472
AcuI CTGAAG 4 cut(s) 354, 634, 753, 991
AfaI GTAC 1 cut(s) 498
AfiI CCNNNNNNNGG 2 cut(s) 55, 288
AflII CTTAAG 1 cut(s) 545
AgsI TTSAA 2 cut(s) 73, 755
AjnI CCWGG 2 cut(s) 168, 339
AluBI AGCT 9 cut(s) 31, 239, 277, 332, 514, 565, 648, 654, 746
AluI AGCT 9 cut(s) 31, 239, 277, 332, 514, 565, 648, 654, 746
Alw21I GWGCWC 2 cut(s) 279, 567
Alw26I GTCTC 2 cut(s) 590, 1006
AlwI GGATC 3 cut(s) 361, 407, 1019
AoxI GGCC 3 cut(s) 80, 111, 337
ApeKI GCWGC 4 cut(s) 125, 236, 239, 654
ApoI RAATTY 2 cut(s) 190, 472
AseI ATTAAT 1 cut(s) 887
Asp700I GAANNNNTTC 1 cut(s) 525
AspLEI GCGC 1 cut(s) 629
AspS9I GGNCC 1 cut(s) 1022
AsuHPI GGTGA 3 cut(s) 43, 199, 500
AsuII TTCGAA 1 cut(s) 224
AvaII GGWCC 1 cut(s) 1022
BanII GRGCYC 3 cut(s) 279, 313, 567
BbsI GAAGAC 2 cut(s) 21, 387
Bbv12I GWGCWC 2 cut(s) 279, 567
BbvI GCAGC 4 cut(s) 112, 226, 248, 641
BccI CCATC 3 cut(s) 268, 290, 390
BciT130I CCWGG 2 cut(s) 170, 341
BcoDI GTCTC 2 cut(s) 590, 1006
BfaI CTAG 1 cut(s) 849
BfmI CTRYAG 1 cut(s) 994
BfrI CTTAAG 1 cut(s) 545
BisI GCNGC 5 cut(s) 126, 237, 240, 655, 666
BlsI GCNGC 5 cut(s) 127, 238, 241, 656, 667
Bme1390I CCNGG 2 cut(s) 170, 341
Bme18I GGWCC 1 cut(s) 1022
BmgT120I GGNCC 1 cut(s) 1022
BmiI GGNNCC 1 cut(s) 312
BmrFI CCNGG 2 cut(s) 170, 341
BmsI GCATC 1 cut(s) 523
BpiI GAAGAC 2 cut(s) 21, 387
Bpu14I TTCGAA 1 cut(s) 224
BsaAI YACGTR 1 cut(s) 496
BsaI GGTCTC 1 cut(s) 590
BsaJI CCNNGG 5 cut(s) 54, 64, 169, 287, 580
BsaWI WCCGGW 1 cut(s) 1056
BsaXI ACNNNNNCTCC 2 cut(s) 986, 1016
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 288
Bse1I ACTGG 2 cut(s) 461, 778
Bse3DI GCAATG 2 cut(s) 384, 660
BseBI CCWGG 2 cut(s) 170, 341
BseDI CCNNGG 5 cut(s) 54, 64, 169, 287, 580
BseGI GGATG 2 cut(s) 179, 282
BseLI CCNNNNNNNGG 2 cut(s) 55, 288
BseMI GCAATG 2 cut(s) 384, 660
BseNI ACTGG 2 cut(s) 461, 778
BseXI GCAGC 4 cut(s) 112, 226, 248, 641
BsgI GTGCAG 2 cut(s) 60, 640
Bsh1236I CGCG 1 cut(s) 1039
BshFI GGCC 3 cut(s) 82, 113, 339
BsiHKAI GWGCWC 2 cut(s) 279, 567
BsiSI CCGG 1 cut(s) 1057
BslFI GGGAC 2 cut(s) 72, 305
BslI CCNNNNNNNGG 2 cut(s) 55, 288
BsmAI GTCTC 2 cut(s) 590, 1006
BsmFI GGGAC 2 cut(s) 72, 305
BsnI GGCC 3 cut(s) 82, 113, 339
Bso31I GGTCTC 1 cut(s) 590
Bsp119I TTCGAA 1 cut(s) 224
Bsp1286I GDGCHC 3 cut(s) 279, 313, 567
Bsp143I GATC 3 cut(s) 353, 399, 1011
Bsp19I CCATGG 2 cut(s) 54, 580
BspACI CCGC 3 cut(s) 666, 943, 1037
BspANI GGCC 3 cut(s) 82, 113, 339
BspFNI CGCG 1 cut(s) 1039
BspHI TCATGA 1 cut(s) 442
BspLI GGNNCC 1 cut(s) 312
BspPI GGATC 3 cut(s) 361, 407, 1019
BspQI GCTCTTC 1 cut(s) 555
BspT104I TTCGAA 1 cut(s) 224
BspTI CTTAAG 1 cut(s) 545
BspTNI GGTCTC 1 cut(s) 590
BsrBI CCGCTC 1 cut(s) 943
BsrDI GCAATG 2 cut(s) 384, 660
BsrI ACTGG 2 cut(s) 461, 778
BssECI CCNNGG 5 cut(s) 54, 64, 169, 287, 580
BssMI GATC 3 cut(s) 353, 399, 1011
BssNAI GTATAC 1 cut(s) 988
BssT1I CCWWGG 4 cut(s) 54, 64, 287, 580
Bst1107I GTATAC 1 cut(s) 988
Bst2UI CCWGG 2 cut(s) 170, 341
Bst4CI ACNGT 2 cut(s) 119, 998
Bst6I CTCTTC 2 cut(s) 455, 555
BstAFI CTTAAG 1 cut(s) 545
BstBAI YACGTR 1 cut(s) 496
BstBI TTCGAA 1 cut(s) 224
BstC8I GCNNGC 2 cut(s) 257, 516
BstDEI CTNAG 1 cut(s) 1047
BstDSI CCRYGG 2 cut(s) 54, 580
BstF5I GGATG 2 cut(s) 179, 282
BstFNI CGCG 1 cut(s) 1039
BstHHI GCGC 1 cut(s) 629
BstKTI GATC 3 cut(s) 356, 402, 1014
BstMAI GTCTC 2 cut(s) 590, 1006
BstMBI GATC 3 cut(s) 353, 399, 1011
BstMWI GCNNNNNNNGC 2 cut(s) 256, 654
BstNI CCWGG 2 cut(s) 170, 341
BstSCI CCNGG 2 cut(s) 168, 339
BstSFI CTRYAG 1 cut(s) 994
BstSNI TACGTA 1 cut(s) 496
BstUI CGCG 1 cut(s) 1039
BstV1I GCAGC 4 cut(s) 112, 226, 248, 641
BstV2I GAAGAC 2 cut(s) 21, 387
BstXI CCANNNNNNTGG 1 cut(s) 306
BstZ17I GTATAC 1 cut(s) 988
BsuRI GGCC 3 cut(s) 82, 113, 339
BtgI CCRYGG 2 cut(s) 54, 580
BtsCI GGATG 2 cut(s) 179, 282
BtsIMutI CAGTG 1 cut(s) 1003
Cac8I GCNNGC 2 cut(s) 257, 516
CciI TCATGA 1 cut(s) 442
CfoI GCGC 1 cut(s) 629
Cfr13I GGNCC 1 cut(s) 1022
Csp6I GTAC 1 cut(s) 497
CviAII CATG 6 cut(s) 55, 325, 443, 581, 596, 728
CviQI GTAC 1 cut(s) 497
DdeI CTNAG 1 cut(s) 1047
DpnI GATC 3 cut(s) 355, 401, 1013
DpnII GATC 3 cut(s) 353, 399, 1011
Eam1104I CTCTTC 2 cut(s) 455, 555
EarI CTCTTC 2 cut(s) 455, 555
Ecl136II GAGCTC 2 cut(s) 277, 565
Eco105I TACGTA 1 cut(s) 496
Eco130I CCWWGG 4 cut(s) 54, 64, 287, 580
Eco147I AGGCCT 1 cut(s) 339
Eco24I GRGCYC 3 cut(s) 279, 313, 567
Eco31I GGTCTC 1 cut(s) 590
Eco47I GGWCC 1 cut(s) 1022
Eco53kI GAGCTC 2 cut(s) 277, 565
Eco57I CTGAAG 4 cut(s) 354, 634, 753, 991
EcoICRI GAGCTC 2 cut(s) 277, 565
EcoRII CCWGG 2 cut(s) 168, 339
EcoT14I CCWWGG 4 cut(s) 54, 64, 287, 580
EcoT38I GRGCYC 3 cut(s) 279, 313, 567
ErhI CCWWGG 4 cut(s) 54, 64, 287, 580
FaeI CATG 6 cut(s) 58, 328, 446, 584, 599, 731
FaqI GGGAC 2 cut(s) 72, 305
FatI CATG 6 cut(s) 54, 324, 442, 580, 595, 727
FauI CCCGC 1 cut(s) 936
FblI GTMKAC 1 cut(s) 987
Fnu4HI GCNGC 5 cut(s) 126, 237, 240, 655, 666
FokI GGATG 2 cut(s) 186, 269
FriOI GRGCYC 3 cut(s) 279, 313, 567
Fsp4HI GCNGC 5 cut(s) 126, 237, 240, 655, 666
FspBI CTAG 1 cut(s) 849
GlaI GCGC 1 cut(s) 628
GluI GCNGC 5 cut(s) 126, 237, 240, 655, 666
HaeIII GGCC 3 cut(s) 82, 113, 339
HapII CCGG 1 cut(s) 1057
HhaI GCGC 1 cut(s) 629
Hin1II CATG 6 cut(s) 58, 328, 446, 584, 599, 731
Hin6I GCGC 1 cut(s) 627
HinP1I GCGC 1 cut(s) 627
HincII GTYRAC 2 cut(s) 553, 906
HindII GTYRAC 2 cut(s) 553, 906
HinfI GANTC 2 cut(s) 60, 526
HpaI GTTAAC 1 cut(s) 553
HpaII CCGG 1 cut(s) 1057
HphI GGTGA 3 cut(s) 43, 199, 500
Hpy166II GTNNAC 3 cut(s) 553, 906, 988
Hpy188I TCNGA 4 cut(s) 145, 404, 784, 895
Hpy188III TCNNGA 6 cut(s) 351, 443, 530, 755, 1009, 1015
Hpy8I GTNNAC 3 cut(s) 553, 906, 988
HpyAV CCTTC 3 cut(s) 329, 626, 1015
HpyCH4III ACNGT 2 cut(s) 119, 998
HpyCH4IV ACGT 2 cut(s) 18, 495
HpyCH4V TGCA 9 cut(s) 41, 95, 128, 242, 536, 657, 680, 707, 822
HpyF10VI GCNNNNNNNGC 2 cut(s) 256, 654
HpyF3I CTNAG 1 cut(s) 1047
HpySE526I ACGT 2 cut(s) 18, 495
Hsp92II CATG 6 cut(s) 58, 328, 446, 584, 599, 731
HspAI GCGC 1 cut(s) 627
KspAI GTTAAC 1 cut(s) 553
Kzo9I GATC 3 cut(s) 353, 399, 1011
LguI GCTCTTC 1 cut(s) 555
LmnI GCTCC 2 cut(s) 316, 940
Lsp1109I GCAGC 4 cut(s) 112, 226, 248, 641
LweI GCATC 1 cut(s) 523
MaeI CTAG 1 cut(s) 849
MaeII ACGT 2 cut(s) 18, 495
MaeIII GTNAC 1 cut(s) 871
MalI GATC 3 cut(s) 355, 401, 1013
MbiI CCGCTC 1 cut(s) 943
MboI GATC 3 cut(s) 353, 399, 1011
MboII GAAGA 4 cut(s) 26, 392, 472, 572
MhlI GDGCHC 3 cut(s) 279, 313, 567
MluCI AATT 9 cut(s) 33, 190, 226, 243, 265, 409, 472, 537, 681
MlyI GAGTC 1 cut(s) 54
MmeI TCCRAC 1 cut(s) 343
MnlI CCTC 6 cut(s) 59, 99, 473, 617, 801, 807
MroXI GAANNNNTTC 1 cut(s) 525
MseI TTAA 3 cut(s) 546, 552, 887
MslI CAYNNNNRTG 3 cut(s) 594, 596, 827
MspA1I CMGCKG 4 cut(s) 239, 514, 654, 668
MspCI CTTAAG 1 cut(s) 545
MspI CCGG 1 cut(s) 1057
MspR9I CCNGG 2 cut(s) 170, 341
MvaI CCWGG 2 cut(s) 170, 341
MvnI CGCG 1 cut(s) 1039
MwoI GCNNNNNNNGC 2 cut(s) 256, 654
NcoI CCATGG 2 cut(s) 54, 580
NdeII GATC 3 cut(s) 353, 399, 1011
NlaIII CATG 6 cut(s) 58, 328, 446, 584, 599, 731
NlaIV GGNNCC 1 cut(s) 312
NspV TTCGAA 1 cut(s) 224
PagI TCATGA 1 cut(s) 442
PceI AGGCCT 1 cut(s) 339
PciSI GCTCTTC 1 cut(s) 555
PdmI GAANNNNTTC 1 cut(s) 525
PfeI GAWTC 1 cut(s) 526
PkrI GCNGC 5 cut(s) 127, 238, 241, 656, 667
PleI GAGTC 1 cut(s) 54
PpsI GAGTC 1 cut(s) 54
Ppu21I YACGTR 1 cut(s) 496
PshBI ATTAAT 1 cut(s) 887
PsiI TTATAA 3 cut(s) 231, 414, 960
Psp124BI GAGCTC 2 cut(s) 279, 567
Psp6I CCWGG 2 cut(s) 168, 339
PspGI CCWGG 2 cut(s) 168, 339
PspN4I GGNNCC 1 cut(s) 312
PspPI GGNCC 1 cut(s) 1022
PvuII CAGCTG 3 cut(s) 239, 514, 654
RsaI GTAC 1 cut(s) 498
RsaNI GTAC 1 cut(s) 497
RseI CAYNNNNRTG 3 cut(s) 594, 596, 827
SacI GAGCTC 2 cut(s) 279, 567
SapI GCTCTTC 1 cut(s) 555
SaqAI TTAA 3 cut(s) 546, 552, 887
SatI GCNGC 5 cut(s) 126, 237, 240, 655, 666
Sau3AI GATC 3 cut(s) 353, 399, 1011
Sau96I GGNCC 1 cut(s) 1022
SchI GAGTC 1 cut(s) 54
ScrFI CCNGG 2 cut(s) 170, 341
SduI GDGCHC 3 cut(s) 279, 313, 567
SfaNI GCATC 1 cut(s) 523
SfcI CTRYAG 1 cut(s) 994
SfuI TTCGAA 1 cut(s) 224
SinI GGWCC 1 cut(s) 1022
SmiMI CAYNNNNRTG 3 cut(s) 594, 596, 827
SmlI CTYRAG 1 cut(s) 545
SmoI CTYRAG 1 cut(s) 545
SnaBI TACGTA 1 cut(s) 496
Sse9I AATT 9 cut(s) 33, 190, 226, 243, 265, 409, 472, 537, 681
SseBI AGGCCT 1 cut(s) 339
SsiI CCGC 3 cut(s) 666, 943, 1037
SspMI CTAG 1 cut(s) 849
SstI GAGCTC 2 cut(s) 279, 567
StuI AGGCCT 1 cut(s) 339
StyD4I CCNGG 2 cut(s) 168, 339
StyI CCWWGG 4 cut(s) 54, 64, 287, 580
TaaI ACNGT 2 cut(s) 119, 998
TaiI ACGT 2 cut(s) 21, 498
TaqI TCGA 2 cut(s) 224, 1014
TasI AATT 9 cut(s) 33, 190, 226, 243, 265, 409, 472, 537, 681
TauI GCSGC 1 cut(s) 668
TfiI GAWTC 1 cut(s) 526
Tru1I TTAA 3 cut(s) 546, 552, 887
Tru9I TTAA 3 cut(s) 546, 552, 887
TscAI CASTG 1 cut(s) 1003
TseI GCWGC 4 cut(s) 125, 236, 239, 654
TspDTI ATGAA 9 cut(s) 27, 41, 431, 459, 573, 584, 706, 804, 978
TspRI CASTG 1 cut(s) 1003
Vha464I CTTAAG 1 cut(s) 545
VpaK11BI GGWCC 1 cut(s) 1022
VspI ATTAAT 1 cut(s) 887
XapI RAATTY 2 cut(s) 190, 472
XmiI GTMKAC 1 cut(s) 987
XmnI GAANNNNTTC 1 cut(s) 525
XspI CTAG 1 cut(s) 849
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.