Rh6AG120500

Thioredoxin-like 1-2, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0017821
Physical Location & Seq
Forward (+)
18046659 .. 18052088
5430 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG120500.1

Sequence Viewer

Length: 579 bp
ATGATAGCTGAAGGTCTGGCAAGGTCTGGATTAAAAGAAGCAAAGTTAGTGCGAGAAGACATTGCTGTAAGGTGGATCAGAACCAATTATAAAGCCTACAAGAAAACAGGTGTCATGCATGAAAAATATGACTGGAAGAGTGTGGAGAATTTTGGAGGTGGTGGTGAATACGTACCCCAGATTTGTCAGCTGGCTGAACAGATTCCAGATGCAATTTTTCTTAAGGTTAACTATGAAGAGCTCAATGCTATGTGCCATGGTCTCCTCATTCATGTGATACCATTCTTTAGGTTTTATAGAGGCGCAGAAGGGCGAGTATGTAGCTTCAGCTGCACCATTGCCACTAGCTGTGCTAATGCAGTTGAGTTCATCATCAACCATGCTGAAGTTCCAATAGCTTTTGTTCAAGAGAACAAAATCCCTGCTATTATATCATGCCTTCCAAACTGTTCTACGCCTTTAAAAACTTCACAAATGTTTCTAGCACTCAAAAGAAGGAAGCAGAAGAACTGGGGGTATCTTGCTTTTCGTGGGAGAAATTCTTTCAGTTGGGAAATTCAGATCATGAACTACCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thioredoxin

Protein Analysis

192

Amino Acids

21.95

Weight (kDa)

9.07

Isoelectric Point (pI)

42.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalase PF01204 1 - 60 5e-11 Trehalase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 90
AclWI GGATC 1 cut(s) 83
AcsI RAATTY 3 cut(s) 148, 538, 555
AcuI CTGAAG 3 cut(s) 30, 310, 405
AfaI GTAC 1 cut(s) 174
AflII CTTAAG 1 cut(s) 221
AgsI TTSAA 1 cut(s) 407
AluBI AGCT 7 cut(s) 8, 190, 241, 324, 330, 348, 398
AluI AGCT 7 cut(s) 8, 190, 241, 324, 330, 348, 398
Alw21I GWGCWC 1 cut(s) 243
Alw26I GTCTC 1 cut(s) 266
AlwI GGATC 1 cut(s) 83
ApeKI GCWGC 1 cut(s) 330
ApoI RAATTY 3 cut(s) 148, 538, 555
Asp700I GAANNNNTTC 1 cut(s) 201
AspLEI GCGC 1 cut(s) 305
AsuHPI GGTGA 1 cut(s) 176
BanII GRGCYC 1 cut(s) 243
BbsI GAAGAC 1 cut(s) 63
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 1 cut(s) 317
BcoDI GTCTC 1 cut(s) 266
BfaI CTAG 2 cut(s) 345, 482
BfrI CTTAAG 1 cut(s) 221
BisI GCNGC 1 cut(s) 331
BlsI GCNGC 1 cut(s) 332
BmrI ACTGGG 1 cut(s) 520
BmsI GCATC 1 cut(s) 199
BmuI ACTGGG 1 cut(s) 520
BpiI GAAGAC 1 cut(s) 63
BsaAI YACGTR 1 cut(s) 172
BsaI GGTCTC 1 cut(s) 266
BsaJI CCNNGG 1 cut(s) 256
Bse1I ACTGG 2 cut(s) 137, 515
Bse3DI GCAATG 2 cut(s) 60, 336
BseDI CCNNGG 1 cut(s) 256
BseMI GCAATG 2 cut(s) 60, 336
BseNI ACTGG 2 cut(s) 137, 515
BseRI GAGGAG 1 cut(s) 254
BseXI GCAGC 1 cut(s) 317
BsgI GTGCAG 1 cut(s) 316
BsiHKAI GWGCWC 1 cut(s) 243
BsmAI GTCTC 1 cut(s) 266
Bso31I GGTCTC 1 cut(s) 266
Bsp1286I GDGCHC 1 cut(s) 243
Bsp143I GATC 2 cut(s) 75, 561
Bsp19I CCATGG 1 cut(s) 256
BspHI TCATGA 1 cut(s) 564
BspPI GGATC 1 cut(s) 83
BspQI GCTCTTC 1 cut(s) 231
BspTI CTTAAG 1 cut(s) 221
BspTNI GGTCTC 1 cut(s) 266
BsrDI GCAATG 2 cut(s) 60, 336
BsrI ACTGG 2 cut(s) 137, 515
BssECI CCNNGG 1 cut(s) 256
BssMI GATC 2 cut(s) 75, 561
BssT1I CCWWGG 1 cut(s) 256
Bst4CI ACNGT 1 cut(s) 449
Bst6I CTCTTC 2 cut(s) 131, 231
BstAFI CTTAAG 1 cut(s) 221
BstBAI YACGTR 1 cut(s) 172
BstC8I GCNNGC 1 cut(s) 192
BstDSI CCRYGG 1 cut(s) 256
BstHHI GCGC 1 cut(s) 305
BstKTI GATC 2 cut(s) 78, 564
BstMAI GTCTC 1 cut(s) 266
BstMBI GATC 2 cut(s) 75, 561
BstMWI GCNNNNNNNGC 1 cut(s) 330
BstSNI TACGTA 1 cut(s) 172
BstV1I GCAGC 1 cut(s) 317
BstV2I GAAGAC 1 cut(s) 63
BtgI CCRYGG 1 cut(s) 256
Cac8I GCNNGC 1 cut(s) 192
CciI TCATGA 1 cut(s) 564
CfoI GCGC 1 cut(s) 305
Csp6I GTAC 1 cut(s) 173
CviAII CATG 7 cut(s) 115, 119, 257, 272, 380, 435, 565
CviJI RGCY 9 cut(s) 8, 95, 190, 194, 241, 324, 330, 348, 398
CviKI_1 RGCY 9 cut(s) 8, 95, 190, 194, 241, 324, 330, 348, 398
CviQI GTAC 1 cut(s) 173
DpnI GATC 2 cut(s) 77, 563
DpnII GATC 2 cut(s) 75, 561
DraI TTTAAA 1 cut(s) 462
Eam1104I CTCTTC 2 cut(s) 131, 231
EarI CTCTTC 2 cut(s) 131, 231
Ecl136II GAGCTC 1 cut(s) 241
Eco105I TACGTA 1 cut(s) 172
Eco130I CCWWGG 1 cut(s) 256
Eco24I GRGCYC 1 cut(s) 243
Eco31I GGTCTC 1 cut(s) 266
Eco53kI GAGCTC 1 cut(s) 241
Eco57I CTGAAG 3 cut(s) 30, 310, 405
EcoICRI GAGCTC 1 cut(s) 241
EcoT14I CCWWGG 1 cut(s) 256
EcoT22I ATGCAT 1 cut(s) 120
EcoT38I GRGCYC 1 cut(s) 243
ErhI CCWWGG 1 cut(s) 256
FaeI CATG 7 cut(s) 118, 122, 260, 275, 383, 438, 568
FatI CATG 7 cut(s) 114, 118, 256, 271, 379, 434, 564
Fnu4HI GCNGC 1 cut(s) 331
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 1 cut(s) 331
FspBI CTAG 2 cut(s) 345, 482
GlaI GCGC 1 cut(s) 304
GluI GCNGC 1 cut(s) 331
HhaI GCGC 1 cut(s) 305
Hin1II CATG 7 cut(s) 118, 122, 260, 275, 383, 438, 568
Hin6I GCGC 1 cut(s) 303
HinP1I GCGC 1 cut(s) 303
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HinfI GANTC 1 cut(s) 202
HpaI GTTAAC 1 cut(s) 229
HphI GGTGA 1 cut(s) 176
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 2 cut(s) 80, 561
Hpy188III TCNNGA 4 cut(s) 27, 206, 407, 565
Hpy8I GTNNAC 1 cut(s) 229
HpyAV CCTTC 4 cut(s) 5, 302, 449, 489
HpyCH4III ACNGT 1 cut(s) 449
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 4 cut(s) 118, 212, 333, 359
HpyF10VI GCNNNNNNNGC 1 cut(s) 330
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 7 cut(s) 118, 122, 260, 275, 383, 438, 568
HspAI GCGC 1 cut(s) 303
KspAI GTTAAC 1 cut(s) 229
Kzo9I GATC 2 cut(s) 75, 561
LguI GCTCTTC 1 cut(s) 231
LpnPI CCDG 9 cut(s) 2, 12, 93, 118, 176, 191, 219, 435, 496
Lsp1109I GCAGC 1 cut(s) 317
LweI GCATC 1 cut(s) 199
MaeI CTAG 2 cut(s) 345, 482
MaeII ACGT 1 cut(s) 171
MalI GATC 2 cut(s) 77, 563
MboI GATC 2 cut(s) 75, 561
MboII GAAGA 4 cut(s) 68, 148, 248, 517
MhlI GDGCHC 1 cut(s) 243
MluCI AATT 5 cut(s) 85, 148, 213, 538, 555
MnlI CCTC 3 cut(s) 149, 275, 293
Mph1103I ATGCAT 1 cut(s) 120
MroXI GAANNNNTTC 1 cut(s) 201
MseI TTAA 4 cut(s) 32, 222, 228, 461
MslI CAYNNNNRTG 1 cut(s) 272
MspA1I CMGCKG 2 cut(s) 190, 330
MspCI CTTAAG 1 cut(s) 221
MwoI GCNNNNNNNGC 1 cut(s) 330
NcoI CCATGG 1 cut(s) 256
NdeII GATC 2 cut(s) 75, 561
NlaIII CATG 7 cut(s) 118, 122, 260, 275, 383, 438, 568
NsiI ATGCAT 1 cut(s) 120
PagI TCATGA 1 cut(s) 564
PciSI GCTCTTC 1 cut(s) 231
PdmI GAANNNNTTC 1 cut(s) 201
PfeI GAWTC 1 cut(s) 202
PkrI GCNGC 1 cut(s) 332
Ppu21I YACGTR 1 cut(s) 172
PsiI TTATAA 1 cut(s) 90
Psp124BI GAGCTC 1 cut(s) 243
PvuII CAGCTG 2 cut(s) 190, 330
RsaI GTAC 1 cut(s) 174
RsaNI GTAC 1 cut(s) 173
RseI CAYNNNNRTG 1 cut(s) 272
SacI GAGCTC 1 cut(s) 243
SapI GCTCTTC 1 cut(s) 231
SaqAI TTAA 4 cut(s) 32, 222, 228, 461
SatI GCNGC 1 cut(s) 331
Sau3AI GATC 2 cut(s) 75, 561
SduI GDGCHC 1 cut(s) 243
SfaNI GCATC 1 cut(s) 199
SmiMI CAYNNNNRTG 1 cut(s) 272
SmlI CTYRAG 1 cut(s) 221
SmoI CTYRAG 1 cut(s) 221
SnaBI TACGTA 1 cut(s) 172
Sse9I AATT 5 cut(s) 85, 148, 213, 538, 555
SspMI CTAG 2 cut(s) 345, 482
SstI GAGCTC 1 cut(s) 243
StyI CCWWGG 1 cut(s) 256
TaaI ACNGT 1 cut(s) 449
TaiI ACGT 1 cut(s) 174
TasI AATT 5 cut(s) 85, 148, 213, 538, 555
TfiI GAWTC 1 cut(s) 202
Tru1I TTAA 4 cut(s) 32, 222, 228, 461
Tru9I TTAA 4 cut(s) 32, 222, 228, 461
TseI GCWGC 1 cut(s) 330
TspDTI ATGAA 4 cut(s) 135, 249, 260, 358
Vha464I CTTAAG 1 cut(s) 221
XapI RAATTY 3 cut(s) 148, 538, 555
XmnI GAANNNNTTC 1 cut(s) 201
XspI CTAG 2 cut(s) 345, 482
Zsp2I ATGCAT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.