Rw2G003830

Thioredoxin-like 1-2, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0017821
Physical Location & Seq
Forward (+)
3639182 .. 3640310
1129 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G003830.1

Sequence Viewer

Length: 390 bp
ATGAAATTTTGCATATGTATAGGGTTTCAGGCACAAGCATCAATCTGTGTAAAACTCATTGATTCTTTGCATAATGCTGGTGATAGATTGGTTGTAGTGGATTTTTGCTTACCTGGTTGTGGAGGTTGTAGAGCTCTCCTTCCCAAGATTTGTCAGCTGGCCGAACAGATTCCAGATGCAATTTTTCTTAAGGTTAACTATGAAGAGATCAAGACTATGTGCCATGGTCTTCACATTCATGTGATACCATTCTTTGGGTTTTATAGAGGCGCAGAAGGGCGAGTATGTAGCTTCAGCTGCACCATTGCCACTATCAAGGAATTCAAAGATGCATTAGCTAAATATGGGACTGAGGGTTGTAGTCTTGGCTCGGCAAAGGGGATTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thioredoxin

Protein Analysis

129

Amino Acids

14.05

Weight (kDa)

8.3

Isoelectric Point (pI)

25.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 27 - 92 5e-11 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 254
AcoI YGGCCR 1 cut(s) 159
AcsI RAATTY 2 cut(s) 5, 320
AcuI CTGAAG 1 cut(s) 277
AfiI CCNNNNNNNGG 2 cut(s) 119, 254
AflII CTTAAG 1 cut(s) 188
AgsI TTSAA 1 cut(s) 325
AjnI CCWGG 1 cut(s) 112
AluBI AGCT 5 cut(s) 134, 157, 291, 297, 338
AluI AGCT 5 cut(s) 134, 157, 291, 297, 338
Alw21I GWGCWC 1 cut(s) 136
AoxI GGCC 1 cut(s) 159
ApeKI GCWGC 1 cut(s) 297
ApoI RAATTY 2 cut(s) 5, 320
Asp700I GAANNNNTTC 1 cut(s) 168
AspLEI GCGC 1 cut(s) 272
AsuHPI GGTGA 1 cut(s) 92
BanII GRGCYC 1 cut(s) 136
BbsI GAAGAC 1 cut(s) 221
Bbv12I GWGCWC 1 cut(s) 136
BbvI GCAGC 1 cut(s) 284
BciT130I CCWGG 1 cut(s) 114
BfrI CTTAAG 1 cut(s) 188
BisI GCNGC 1 cut(s) 298
BlsI GCNGC 1 cut(s) 299
Bme1390I CCNGG 1 cut(s) 114
BmrFI CCNGG 1 cut(s) 114
BmsI GCATC 3 cut(s) 47, 166, 319
BpiI GAAGAC 1 cut(s) 221
BsaJI CCNNGG 1 cut(s) 223
Bsc4I CCNNNNNNNGG 2 cut(s) 119, 254
Bse3DI GCAATG 1 cut(s) 303
BseBI CCWGG 1 cut(s) 114
BseDI CCNNGG 1 cut(s) 223
BseLI CCNNNNNNNGG 2 cut(s) 119, 254
BseMI GCAATG 1 cut(s) 303
BseMII CTCAG 1 cut(s) 342
BseXI GCAGC 1 cut(s) 284
BsgI GTGCAG 1 cut(s) 283
BshFI GGCC 1 cut(s) 161
BsiHKAI GWGCWC 1 cut(s) 136
BslFI GGGAC 1 cut(s) 361
BslI CCNNNNNNNGG 2 cut(s) 119, 254
BsmFI GGGAC 1 cut(s) 361
BsnI GGCC 1 cut(s) 161
Bsp1286I GDGCHC 1 cut(s) 136
Bsp143I GATC 1 cut(s) 207
Bsp19I CCATGG 1 cut(s) 223
BspANI GGCC 1 cut(s) 161
BspCNI CTCAG 1 cut(s) 343
BspTI CTTAAG 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 303
BssECI CCNNGG 1 cut(s) 223
BssMI GATC 1 cut(s) 207
BssT1I CCWWGG 1 cut(s) 223
Bst2UI CCWGG 1 cut(s) 114
Bst6I CTCTTC 1 cut(s) 198
BstAFI CTTAAG 1 cut(s) 188
BstC8I GCNNGC 1 cut(s) 159
BstDEI CTNAG 1 cut(s) 351
BstDSI CCRYGG 1 cut(s) 223
BstHHI GCGC 1 cut(s) 272
BstKTI GATC 1 cut(s) 210
BstMBI GATC 1 cut(s) 207
BstMWI GCNNNNNNNGC 1 cut(s) 297
BstNI CCWGG 1 cut(s) 114
BstSCI CCNGG 1 cut(s) 112
BstV1I GCAGC 1 cut(s) 284
BstV2I GAAGAC 1 cut(s) 221
BsuRI GGCC 1 cut(s) 161
BtgI CCRYGG 1 cut(s) 223
Cac8I GCNNGC 1 cut(s) 159
CfoI GCGC 1 cut(s) 272
CsiI ACCWGGT 1 cut(s) 112
CviAII CATG 2 cut(s) 224, 239
CviJI RGCY 7 cut(s) 134, 157, 161, 291, 297, 338, 369
CviKI_1 RGCY 7 cut(s) 134, 157, 161, 291, 297, 338, 369
DdeI CTNAG 1 cut(s) 351
DpnI GATC 1 cut(s) 209
DpnII GATC 1 cut(s) 207
EaeI YGGCCR 1 cut(s) 159
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
Ecl136II GAGCTC 1 cut(s) 134
Eco130I CCWWGG 1 cut(s) 223
Eco24I GRGCYC 1 cut(s) 136
Eco53kI GAGCTC 1 cut(s) 134
Eco57I CTGAAG 1 cut(s) 277
EcoICRI GAGCTC 1 cut(s) 134
EcoRI GAATTC 1 cut(s) 320
EcoRII CCWGG 1 cut(s) 112
EcoT14I CCWWGG 1 cut(s) 223
EcoT22I ATGCAT 1 cut(s) 334
EcoT38I GRGCYC 1 cut(s) 136
ErhI CCWWGG 1 cut(s) 223
FaeI CATG 2 cut(s) 227, 242
FaqI GGGAC 1 cut(s) 361
FatI CATG 2 cut(s) 223, 238
FauNDI CATATG 1 cut(s) 14
Fnu4HI GCNGC 1 cut(s) 298
FriOI GRGCYC 1 cut(s) 136
Fsp4HI GCNGC 1 cut(s) 298
GlaI GCGC 1 cut(s) 271
GluI GCNGC 1 cut(s) 298
HaeIII GGCC 1 cut(s) 161
HhaI GCGC 1 cut(s) 272
Hin1II CATG 2 cut(s) 227, 242
Hin6I GCGC 1 cut(s) 270
HinP1I GCGC 1 cut(s) 270
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HinfI GANTC 2 cut(s) 62, 169
HpaI GTTAAC 1 cut(s) 196
HphI GGTGA 1 cut(s) 92
Hpy166II GTNNAC 1 cut(s) 196
Hpy188III TCNNGA 2 cut(s) 173, 211
Hpy8I GTNNAC 1 cut(s) 196
HpyAV CCTTC 2 cut(s) 149, 269
HpyCH4V TGCA 5 cut(s) 12, 70, 179, 300, 332
HpyF10VI GCNNNNNNNGC 1 cut(s) 297
HpyF3I CTNAG 1 cut(s) 351
Hsp92II CATG 2 cut(s) 227, 242
HspAI GCGC 1 cut(s) 270
KspAI GTTAAC 1 cut(s) 196
Kzo9I GATC 1 cut(s) 207
LpnPI CCDG 6 cut(s) 14, 63, 99, 126, 143, 186
Lsp1109I GCAGC 1 cut(s) 284
LweI GCATC 3 cut(s) 47, 166, 319
MabI ACCWGGT 1 cut(s) 112
MalI GATC 1 cut(s) 209
MboI GATC 1 cut(s) 207
MboII GAAGA 2 cut(s) 215, 221
MhlI GDGCHC 1 cut(s) 136
MluCI AATT 3 cut(s) 5, 180, 320
MnlI CCTC 3 cut(s) 116, 260, 346
Mph1103I ATGCAT 1 cut(s) 334
MroXI GAANNNNTTC 1 cut(s) 168
MseI TTAA 2 cut(s) 189, 195
MslI CAYNNNNRTG 2 cut(s) 237, 239
MspA1I CMGCKG 2 cut(s) 157, 297
MspCI CTTAAG 1 cut(s) 188
MspR9I CCNGG 1 cut(s) 114
MvaI CCWGG 1 cut(s) 114
MwoI GCNNNNNNNGC 1 cut(s) 297
NcoI CCATGG 1 cut(s) 223
NdeI CATATG 1 cut(s) 14
NdeII GATC 1 cut(s) 207
NlaIII CATG 2 cut(s) 227, 242
NmeAIII GCCGAG 1 cut(s) 350
NsiI ATGCAT 1 cut(s) 334
PdmI GAANNNNTTC 1 cut(s) 168
PfeI GAWTC 2 cut(s) 62, 169
PflMI CCANNNNNTGG 1 cut(s) 254
PkrI GCNGC 1 cut(s) 299
Psp124BI GAGCTC 1 cut(s) 136
Psp6I CCWGG 1 cut(s) 112
PspGI CCWGG 1 cut(s) 112
PvuII CAGCTG 2 cut(s) 157, 297
RseI CAYNNNNRTG 2 cut(s) 237, 239
SacI GAGCTC 1 cut(s) 136
SaqAI TTAA 2 cut(s) 189, 195
SatI GCNGC 1 cut(s) 298
Sau3AI GATC 1 cut(s) 207
ScrFI CCNGG 1 cut(s) 114
SduI GDGCHC 1 cut(s) 136
SetI ASST 8 cut(s) 115, 127, 136, 159, 195, 293, 299, 340
SexAI ACCWGGT 1 cut(s) 112
SfaNI GCATC 3 cut(s) 47, 166, 319
SmiMI CAYNNNNRTG 2 cut(s) 237, 239
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
Sse9I AATT 3 cut(s) 5, 180, 320
SstI GAGCTC 1 cut(s) 136
StyD4I CCNGG 1 cut(s) 112
StyI CCWWGG 1 cut(s) 223
TasI AATT 3 cut(s) 5, 180, 320
TfiI GAWTC 2 cut(s) 62, 169
Tru1I TTAA 2 cut(s) 189, 195
Tru9I TTAA 2 cut(s) 189, 195
TseI GCWGC 1 cut(s) 297
TspDTI ATGAA 3 cut(s) 17, 216, 227
Van91I CCANNNNNTGG 1 cut(s) 254
Vha464I CTTAAG 1 cut(s) 188
XapI RAATTY 2 cut(s) 5, 320
XmnI GAANNNNTTC 1 cut(s) 168
Zsp2I ATGCAT 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.