RchiOBHm_Chr6g0292651

ASCH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0005025
Physical Location & Seq
Forward (+)
55458243 .. 55459228
986 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 738 bp
ATGTGTGTGCCATCTGTGTTAGAGAATCAAGCAATAAAAATGGTTGAATCCAAAGTGAAAGAGTGCATGGAAGAGCTGTTGAAGTTCACCCTTGAATCTCACATAGAGGGTACCCAGGATATCGATCTAGCCTTGCCCACTGACTTCTGCCTCCTCAACAACCCGAAAATCGGCACCAATTCTACAAAGGGAGAACCCCCATATCCTTTATACAAGCAACTAGCACTAGCCCTATATGAATCCATCAATTCTAATGCCATTTCAGGGACAGATAATTGGGATCAGAGTTCACAATTACTAAACACTCTCAAGTCTGTGGACTTTGAACTCCATGTTCAGGAGCCCTTTTTCACACAAATCAAAGACGGCCTAAAGACAGTCGAAGGAAGGTGCAACGTTGGTCCCTATAGTCGAATCGGATCAGGAGCACTGATCCTCTTCAACAAATGCCTGATTGTCGAGGTTAAGGAAGTCCGATCGTATGGTTCCTTCTCTGATATGTTGCAAGCAGAGAGCCTAGAGAAAGTTCTCCCTGGTGTGAAAACCATTGAAGAGGGTGTTCAAGTCTACAGAAGATTCTACACTGAAGAAATTGAAAGGTCCAATGGTGTCATTGCCATATGCATTGAAAAATTGCCTGCCCAACCCTATATGTATTTGGCCAAGTTATTATCAGAATTGGGTAATGATGGTATTCAAAGCCTTCTGGAGCTCAATTCACATGAATTCAGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ASCH

Protein Analysis

245

Amino Acids

27.38

Weight (kDa)

4.8

Isoelectric Point (pI)

51.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ASCH PF04266 110 - 211 7.7e-16 ASCH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 110
AccB1I GGYRCC 2 cut(s) 110, 173
AccI GTMKAC 1 cut(s) 567
AclI AACGTT 1 cut(s) 396
AclWI GGATC 3 cut(s) 288, 427, 427
AcoI YGGCCR 1 cut(s) 660
AcsI RAATTY 1 cut(s) 725
AcuI CTGAAG 1 cut(s) 606
AfaI GTAC 1 cut(s) 112
AfiI CCNNNNNNNGG 3 cut(s) 170, 264, 337
AjnI CCWGG 2 cut(s) 114, 532
AluBI AGCT 2 cut(s) 76, 712
AluI AGCT 2 cut(s) 76, 712
Alw21I GWGCWC 2 cut(s) 430, 714
AlwI GGATC 3 cut(s) 288, 427, 427
AoxI GGCC 2 cut(s) 367, 660
ApoI RAATTY 1 cut(s) 725
Asp718I GGTACC 1 cut(s) 110
AspS9I GGNCC 2 cut(s) 401, 600
AsuHPI GGTGA 1 cut(s) 79
AvaII GGWCC 2 cut(s) 401, 600
BalI TGGCCA 1 cut(s) 662
BanI GGYRCC 2 cut(s) 110, 173
BanII GRGCYC 2 cut(s) 345, 714
BarI GAAGNNNNNNTAC 2 cut(s) 473, 505
Bbv12I GWGCWC 2 cut(s) 430, 714
BccI CCATC 3 cut(s) 19, 251, 683
BceAI ACGGC 1 cut(s) 382
BciT130I CCWGG 2 cut(s) 116, 534
BfaI CTAG 4 cut(s) 128, 221, 227, 518
BfmI CTRYAG 2 cut(s) 406, 568
Bme1390I CCNGG 2 cut(s) 116, 534
Bme18I GGWCC 2 cut(s) 401, 600
BmgT120I GGNCC 2 cut(s) 401, 600
BmiI GGNNCC 5 cut(s) 112, 175, 342, 403, 487
BmrFI CCNGG 2 cut(s) 116, 534
BpmI CTGGAG 1 cut(s) 728
BpuEI CTTGAG 1 cut(s) 293
Bsa29I ATCGAT 1 cut(s) 123
BsaBI GATNNNNATC 1 cut(s) 123
BsaJI CCNNGG 2 cut(s) 114, 532
Bsc4I CCNNNNNNNGG 3 cut(s) 170, 264, 337
Bse3DI GCAATG 1 cut(s) 612
Bse8I GATNNNNATC 1 cut(s) 123
BseBI CCWGG 2 cut(s) 116, 534
BseCI ATCGAT 1 cut(s) 123
BseDI CCNNGG 2 cut(s) 114, 532
BseJI GATNNNNATC 1 cut(s) 123
BseLI CCNNNNNNNGG 3 cut(s) 170, 264, 337
BseMI GCAATG 1 cut(s) 612
BseRI GAGGAG 1 cut(s) 143
Bsh1285I CGRYCG 1 cut(s) 479
BshFI GGCC 2 cut(s) 369, 662
BshNI GGYRCC 2 cut(s) 110, 173
BshVI ATCGAT 1 cut(s) 123
BsiEI CGRYCG 1 cut(s) 479
BsiHKAI GWGCWC 2 cut(s) 430, 714
BslFI GGGAC 2 cut(s) 280, 387
BslI CCNNNNNNNGG 3 cut(s) 170, 264, 337
BsmFI GGGAC 2 cut(s) 280, 387
BsnI GGCC 2 cut(s) 369, 662
Bsp1286I GDGCHC 3 cut(s) 345, 430, 714
Bsp143I GATC 5 cut(s) 124, 280, 419, 432, 476
BspANI GGCC 2 cut(s) 369, 662
BspDI ATCGAT 1 cut(s) 123
BspLI GGNNCC 5 cut(s) 112, 175, 342, 403, 487
BspPI GGATC 3 cut(s) 288, 427, 427
BspQI GCTCTTC 1 cut(s) 66
BspT107I GGYRCC 2 cut(s) 110, 173
BsrDI GCAATG 1 cut(s) 612
BssECI CCNNGG 2 cut(s) 114, 532
BssMI GATC 5 cut(s) 124, 280, 419, 432, 476
Bst2UI CCWGG 2 cut(s) 116, 534
Bst4CI ACNGT 1 cut(s) 379
Bst6I CTCTTC 3 cut(s) 66, 443, 546
BstC8I GCNNGC 2 cut(s) 507, 639
BstKTI GATC 5 cut(s) 127, 283, 422, 435, 479
BstMBI GATC 5 cut(s) 124, 280, 419, 432, 476
BstMCI CGRYCG 1 cut(s) 479
BstNI CCWGG 2 cut(s) 116, 534
BstSCI CCNGG 2 cut(s) 114, 532
BstSFI CTRYAG 2 cut(s) 406, 568
Bsu15I ATCGAT 1 cut(s) 123
BsuRI GGCC 2 cut(s) 369, 662
BsuTUI ATCGAT 1 cut(s) 123
BtsIMutI CAGTG 4 cut(s) 138, 428, 582, 736
Cac8I GCNNGC 2 cut(s) 507, 639
Cfr13I GGNCC 2 cut(s) 401, 600
ClaI ATCGAT 1 cut(s) 123
Csp6I GTAC 1 cut(s) 111
CviAII CATG 3 cut(s) 67, 332, 722
CviJI RGCY 9 cut(s) 76, 131, 230, 343, 369, 516, 662, 702, 712
CviKI_1 RGCY 9 cut(s) 76, 131, 230, 343, 369, 516, 662, 702, 712
CviQI GTAC 1 cut(s) 111
DpnI GATC 5 cut(s) 126, 282, 421, 434, 478
DpnII GATC 5 cut(s) 124, 280, 419, 432, 476
EaeI YGGCCR 1 cut(s) 660
Eam1104I CTCTTC 3 cut(s) 66, 443, 546
EarI CTCTTC 3 cut(s) 66, 443, 546
Ecl136II GAGCTC 1 cut(s) 712
Eco24I GRGCYC 2 cut(s) 345, 714
Eco32I GATATC 1 cut(s) 121
Eco47I GGWCC 2 cut(s) 401, 600
Eco53kI GAGCTC 1 cut(s) 712
Eco57I CTGAAG 1 cut(s) 606
EcoICRI GAGCTC 1 cut(s) 712
EcoRI GAATTC 1 cut(s) 725
EcoRII CCWGG 2 cut(s) 114, 532
EcoRV GATATC 1 cut(s) 121
EcoT22I ATGCAT 1 cut(s) 626
EcoT38I GRGCYC 2 cut(s) 345, 714
FaeI CATG 3 cut(s) 70, 335, 725
FaqI GGGAC 2 cut(s) 280, 387
FatI CATG 3 cut(s) 66, 331, 721
FauNDI CATATG 1 cut(s) 620
FblI GTMKAC 1 cut(s) 567
FriOI GRGCYC 2 cut(s) 345, 714
FspBI CTAG 4 cut(s) 128, 221, 227, 518
GsuI CTGGAG 1 cut(s) 728
HaeIII GGCC 2 cut(s) 369, 662
Hin1II CATG 3 cut(s) 70, 335, 725
HinfI GANTC 6 cut(s) 25, 47, 95, 239, 414, 576
HphI GGTGA 1 cut(s) 79
Hpy166II GTNNAC 4 cut(s) 87, 290, 319, 568
Hpy188I TCNGA 5 cut(s) 285, 419, 476, 496, 676
Hpy188III TCNNGA 3 cut(s) 338, 423, 707
Hpy8I GTNNAC 4 cut(s) 87, 290, 319, 568
HpyAV CCTTC 4 cut(s) 377, 381, 499, 713
HpyCH4III ACNGT 1 cut(s) 379
HpyCH4IV ACGT 1 cut(s) 396
HpyCH4V TGCA 5 cut(s) 66, 393, 505, 624, 734
HpySE526I ACGT 1 cut(s) 396
Hsp92II CATG 3 cut(s) 70, 335, 725
KpnI GGTACC 1 cut(s) 114
Kzo9I GATC 5 cut(s) 124, 280, 419, 432, 476
LguI GCTCTTC 1 cut(s) 66
LmnI GCTCC 3 cut(s) 340, 425, 709
MaeI CTAG 4 cut(s) 128, 221, 227, 518
MaeII ACGT 1 cut(s) 396
MalI GATC 5 cut(s) 126, 282, 421, 434, 478
MboI GATC 5 cut(s) 124, 280, 419, 432, 476
MboII GAAGA 5 cut(s) 83, 430, 563, 585, 599
MhlI GDGCHC 3 cut(s) 345, 430, 714
MlsI TGGCCA 1 cut(s) 662
MluCI AATT 9 cut(s) 178, 247, 274, 293, 591, 632, 677, 715, 725
MluNI TGGCCA 1 cut(s) 662
MnlI CCTC 6 cut(s) 100, 161, 164, 446, 454, 547
Mox20I TGGCCA 1 cut(s) 662
Mph1103I ATGCAT 1 cut(s) 626
MscI TGGCCA 1 cut(s) 662
MseI TTAA 1 cut(s) 465
Msp20I TGGCCA 1 cut(s) 662
MspR9I CCNGG 2 cut(s) 116, 534
MvaI CCWGG 2 cut(s) 116, 534
NdeI CATATG 1 cut(s) 620
NdeII GATC 5 cut(s) 124, 280, 419, 432, 476
NlaIII CATG 3 cut(s) 70, 335, 725
NlaIV GGNNCC 5 cut(s) 112, 175, 342, 403, 487
NsiI ATGCAT 1 cut(s) 626
PciSI GCTCTTC 1 cut(s) 66
PfeI GAWTC 6 cut(s) 25, 47, 95, 239, 414, 576
Ple19I CGATCG 1 cut(s) 479
Psp124BI GAGCTC 1 cut(s) 714
Psp1406I AACGTT 1 cut(s) 396
Psp6I CCWGG 2 cut(s) 114, 532
PspGI CCWGG 2 cut(s) 114, 532
PspN4I GGNNCC 5 cut(s) 112, 175, 342, 403, 487
PspPI GGNCC 2 cut(s) 401, 600
PvuI CGATCG 1 cut(s) 479
RsaI GTAC 1 cut(s) 112
RsaNI GTAC 1 cut(s) 111
SacI GAGCTC 1 cut(s) 714
SapI GCTCTTC 1 cut(s) 66
SaqAI TTAA 1 cut(s) 465
Sau3AI GATC 5 cut(s) 124, 280, 419, 432, 476
Sau96I GGNCC 2 cut(s) 401, 600
ScrFI CCNGG 2 cut(s) 116, 534
SduI GDGCHC 3 cut(s) 345, 430, 714
SetI ASST 6 cut(s) 78, 392, 399, 465, 602, 714
SfcI CTRYAG 2 cut(s) 406, 568
SinI GGWCC 2 cut(s) 401, 600
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
Sse9I AATT 9 cut(s) 178, 247, 274, 293, 591, 632, 677, 715, 725
SspMI CTAG 4 cut(s) 128, 221, 227, 518
SstI GAGCTC 1 cut(s) 714
StyD4I CCNGG 2 cut(s) 114, 532
TaaI ACNGT 1 cut(s) 379
TaiI ACGT 1 cut(s) 399
TaqI TCGA 4 cut(s) 123, 381, 412, 459
TasI AATT 9 cut(s) 178, 247, 274, 293, 591, 632, 677, 715, 725
TfiI GAWTC 6 cut(s) 25, 47, 95, 239, 414, 576
Tru1I TTAA 1 cut(s) 465
Tru9I TTAA 1 cut(s) 465
TscAI CASTG 4 cut(s) 145, 435, 589, 736
TspDTI ATGAA 2 cut(s) 252, 738
TspRI CASTG 4 cut(s) 145, 435, 589, 736
VpaK11BI GGWCC 2 cut(s) 401, 600
XapI RAATTY 1 cut(s) 725
XmiI GTMKAC 1 cut(s) 567
XspI CTAG 4 cut(s) 128, 221, 227, 518
Zsp2I ATGCAT 1 cut(s) 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.