RchiOBHm_Chr6g0295411

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0015200
Physical Location & Seq
Forward (+)
57420761 .. 57422308
1548 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1548 bp
ATGACAGAGTTACTCATGCTAAGCTCAGCTATGGAGCTTGAGCTCGACCTCGACCTCGACCTCTCCGGCTGCAGCACTGAAACCACAAGCACGACCAACACGGCCATTTCCCCAGACCAGTGGTACGATTGGTCTCCGGTAGTCGACTGGGAGTCTCTCGCTCCGATTGACCAGCTCGCCGGCGGTGGAGACTTTCACGAGCTGATCGAGTCGATGATGGAGGGCCGAGACGGCTGCGAGATGGACTCGCCGTCGTCCGAGACCATTGAGGAAGCGTCAAACTCGTGTTACACCACGACGTCGTCGCAGTCGACGGACGTGACGATGCTGGAGGAAGGCGTGGCATCGAACGGCGAGGATTCGAAGGGGCTGAGATTGGTGCATCTGTTGATGGCGGCGGCGGAGGCGCTGACTGGCGCCAACAAGAGCAGAGATCTGGCTCGGGTGATATTGGTTCGGCTCAAGGAGTTGGTCTCCCCAACTGACGGTACTAACATGGAGAGGCTGGCGGCGTATTTTACCGAGGCGCTTCAGGGTTTGCTAGAAGGCGCCGGAGGTGTCAGCCACTCCAAGCATTTGTTTGGAAATGGGTCCCACCGCGAGCACGGTCACCATCCGACGACGGACGTGATGGCGGCGTTTCAGCTACTTCAGGACATGTCGCCCTATGTCAAGTTTGGGCACTTCACGGCCAATCAGGCCATTTTGGAGGCTGTGGCCCATGACAGGAGGGTCCACATAGTGGACTATGACATCATGGAGGGAATCCAATGGGCCTCCTTGATGCAGGCCTTGGTGTCCAGGAAGGATGGCCCACCGACCCCACATCTTAGGATCACTGCTTTGTCGCGAGGCGGGAGCTGCCGGAGGTCGATAGGGACCGTCCAAGAGACCGGTCGTCGTCTCACGGCGTTTGCCACGTCCATTGGTCAACCGTTTTCGTTTCATCAATGTAGGTTGGATTCGGACGAGACATTTCGACCGTCTGCTCTAAAGTTGGTTAAGGGCGAGGCCTTGGTAATAAACTGCATGCTGAACCTTCCACACTTTAGTTATCGATCCCCGGATTCGATTGCTTCCTTTTTATCTGGAGCGAAAACCCTAAACCCGAGACTGGTAACTTTGGTTGAAGAAGAGGTTAGGCCTGCCAGAGACGGTGGCTTTGTGGCCCGCTTCATGGACTCATTGTATCACTACTCGGCTGTCTACGACTCGCTCGAGGCCGGCTTCCCAATGCAGAGCCGGGCTCGAGAATTAGTGGAGAGAGTGTTCTTGGGACCCCGAATAGCTGGGTCGTTGGCTCGTATCTATCGGGCTCAAGGAGAGGTAGGCTGCTCTTGGTGGGAGTGGTTAGGTGCGATAGGGTTTAAGCCAATGCCAATAAGCTTTGCCAATCATTGTCAGGCAAAGCTTTTGTTGGGTCTTTTTAATGACGGTTATAGGGTGGAGGAGGTGGCAAATCATAGGTTGGTTTTAGGTTGGAAATCACGGTCCTTACTATCAGCTTCTATTTGGACTTCACCCCCACAATCTGATTTTGCTGATTAG

Protein Analysis

515

Amino Acids

56.64

Weight (kDa)

5.26

Isoelectric Point (pI)

43.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 126 - 506 5.1e-104 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015200)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08250
fragaria_vesca FvH4_2g26540
malus_domestica MD08G1167600.v1.1 MD15G1353200.v1.1
prunus_persica Prupe.1G501500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0295411
rosa_laevigata RLG00000011806
rosa_multiflora Rmu_co8465007.1_g000001 Rmu_sc0003058.1_g000004
rosa_roxburghii Rroxscaffold_7G00172520
rosa_rugosa Rorug06G0250400
rosa_samantha Rh6AG363800 Rh6BG372000 Rh6CG378400 Rh6DG364800
rosa_wichuraiana Rw6G031760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 731
AatII GACGTC 1 cut(s) 302
AccB1I GGYRCC 2 cut(s) 416, 548
AccB7I CCANNNNNTGG 1 cut(s) 742
AccI GTMKAC 3 cut(s) 144, 311, 1206
AccII CGCG 2 cut(s) 600, 850
AciI CCGC 9 cut(s) 183, 395, 398, 401, 509, 598, 635, 855, 1171
AclWI GGATC 2 cut(s) 842, 1053
AcoI YGGCCR 2 cut(s) 102, 690
AcuI CTGAAG 2 cut(s) 515, 635
AcyI GRCGYC 3 cut(s) 299, 417, 549
AdeI CACNNNGTG 1 cut(s) 742
AfaI GTAC 2 cut(s) 125, 490
AfiI CCNNNNNNNGG 5 cut(s) 485, 726, 742, 1114, 1177
AflIII ACRYGT 1 cut(s) 657
AgeI ACCGGT 1 cut(s) 893
AgsI TTSAA 1 cut(s) 1130
AhdI GACNNNNNGTC 3 cut(s) 151, 250, 897
AjiI CACGTC 3 cut(s) 319, 628, 921
AjnI CCWGG 1 cut(s) 800
AloI GAACNNNNNNTCC 2 cut(s) 1253, 1285
Alw21I GWGCWC 2 cut(s) 45, 606
AlwI GGATC 2 cut(s) 842, 1053
Ama87I CYCGRG 4 cut(s) 441, 1108, 1217, 1248
ApeKI GCWGC 5 cut(s) 69, 72, 234, 861, 1332
ArsI GACNNNNNNTTYG 2 cut(s) 1145, 1177
AsiGI ACCGGT 1 cut(s) 893
AspLEI GCGC 4 cut(s) 409, 419, 529, 551
AsuC2I CCSGG 2 cut(s) 1064, 1244
AsuHPI GGTGA 3 cut(s) 457, 602, 1512
AsuII TTCGAA 1 cut(s) 362
AvaI CYCGRG 4 cut(s) 441, 1108, 1217, 1248
AvaII GGWCC 5 cut(s) 591, 733, 879, 1277, 1491
BaeGI GKGCMC 1 cut(s) 684
BanI GGYRCC 2 cut(s) 416, 548
BanII GRGCYC 3 cut(s) 45, 1249, 1318
BauI CACGAG 2 cut(s) 197, 283
Bbv12I GWGCWC 2 cut(s) 45, 606
BbvI GCAGC 5 cut(s) 56, 84, 221, 848, 1319
BccI CCATC 6 cut(s) 211, 235, 385, 621, 625, 803
BceAI ACGGC 6 cut(s) 117, 235, 247, 367, 705, 924
BcgI CGANNNNNNTGC 2 cut(s) 216, 250
BciT130I CCWGG 1 cut(s) 802
BcnI CCSGG 2 cut(s) 1064, 1244
BfaI CTAG 1 cut(s) 542
BfmI CTRYAG 1 cut(s) 70
BfoI RGCGCY 4 cut(s) 410, 420, 530, 552
BglI GCCNNNNNGGC 2 cut(s) 231, 698
BglII AGATCT 1 cut(s) 433
BisI GCNGC 9 cut(s) 70, 73, 235, 396, 399, 510, 636, 862, 1333
BlpI GCTNAGC 2 cut(s) 20, 25
BlsI GCNGC 9 cut(s) 71, 74, 236, 397, 400, 511, 637, 863, 1334
Bme1390I CCNGG 3 cut(s) 802, 1064, 1244
Bme18I GGWCC 5 cut(s) 591, 733, 879, 1277, 1491
BmeRI GACNNNNNGTC 3 cut(s) 151, 250, 897
BmeT110I CYCGRG 4 cut(s) 441, 1108, 1217, 1248
BmgBI CACGTC 3 cut(s) 319, 628, 921
BmiI GGNNCC 8 cut(s) 418, 550, 592, 593, 734, 880, 1278, 1279
BmrFI CCNGG 3 cut(s) 802, 1064, 1244
BmrI ACTGGG 1 cut(s) 157
BmsI GCATC 4 cut(s) 315, 353, 391, 774
BmuI ACTGGG 1 cut(s) 157
BplI GAGNNNNNCTC 6 cut(s) 230, 262, 458, 490, 1231, 1263
BpmI CTGGAG 2 cut(s) 350, 1110
Bpu1102I GCTNAGC 2 cut(s) 20, 25
Bpu14I TTCGAA 1 cut(s) 362
BpuEI CTTGAG 3 cut(s) 59, 446, 1302
BpuMI CCSGG 2 cut(s) 1064, 1244
Bsa29I ATCGAT 1 cut(s) 1057
BsaHI GRCGYC 3 cut(s) 299, 417, 549
BsaI GGTCTC 4 cut(s) 138, 254, 478, 884
BsaJI CCNNGG 4 cut(s) 522, 792, 1014, 1062
BsaWI WCCGGW 2 cut(s) 136, 893
BsaXI ACNNNNNCTCC 2 cut(s) 1253, 1283
Bsc4I CCNNNNNNNGG 5 cut(s) 485, 726, 742, 1114, 1177
Bse118I RCCGGY 3 cut(s) 179, 893, 1223
Bse1I ACTGG 4 cut(s) 118, 152, 418, 1119
BseBI CCWGG 1 cut(s) 802
BseCI ATCGAT 1 cut(s) 1057
BseDI CCNNGG 4 cut(s) 522, 792, 1014, 1062
BseGI GGATG 2 cut(s) 613, 814
BseLI CCNNNNNNNGG 5 cut(s) 485, 726, 742, 1114, 1177
BseMII CTCAG 2 cut(s) 39, 362
BseNI ACTGG 4 cut(s) 118, 152, 418, 1119
BseRI GAGGAG 1 cut(s) 1463
BseSI GKGCMC 1 cut(s) 684
BseXI GCAGC 5 cut(s) 56, 84, 221, 848, 1319
BseYI CCCAGC 1 cut(s) 1289
Bsh1236I CGCG 2 cut(s) 600, 850
Bsh1285I CGRYCG 2 cut(s) 898, 983
BshNI GGYRCC 2 cut(s) 416, 548
BshTI ACCGGT 1 cut(s) 893
BshVI ATCGAT 1 cut(s) 1057
BsiEI CGRYCG 2 cut(s) 898, 983
BsiHKAI GWGCWC 2 cut(s) 45, 606
BsiHKCI CYCGRG 4 cut(s) 441, 1108, 1217, 1248
BsiSI CCGG 9 cut(s) 66, 137, 180, 552, 865, 894, 1064, 1224, 1243
BslFI GGGAC 3 cut(s) 577, 892, 1290
BslI CCNNNNNNNGG 5 cut(s) 485, 726, 742, 1114, 1177
BsmBI CGTCTC 3 cut(s) 222, 908, 1146
BsmFI GGGAC 3 cut(s) 577, 892, 1290
Bso31I GGTCTC 4 cut(s) 138, 254, 478, 884
BsoBI CYCGRG 4 cut(s) 441, 1108, 1217, 1248
Bsp119I TTCGAA 1 cut(s) 362
Bsp1286I GDGCHC 5 cut(s) 45, 606, 684, 1249, 1318
Bsp143I GATC 4 cut(s) 204, 433, 834, 1058
Bsp1720I GCTNAGC 2 cut(s) 20, 25
Bsp68I TCGCGA 1 cut(s) 850
BspACI CCGC 9 cut(s) 183, 395, 398, 401, 509, 598, 635, 855, 1171
BspCNI CTCAG 2 cut(s) 38, 363
BspDI ATCGAT 1 cut(s) 1057
BspFNI CGCG 2 cut(s) 600, 850
BspLI GGNNCC 8 cut(s) 418, 550, 592, 593, 734, 880, 1278, 1279
BspMAI CTGCAG 1 cut(s) 74
BspPI GGATC 2 cut(s) 842, 1053
BspT104I TTCGAA 1 cut(s) 362
BspT107I GGYRCC 2 cut(s) 416, 548
BspTNI GGTCTC 4 cut(s) 138, 254, 478, 884
BsrFI RCCGGY 3 cut(s) 179, 893, 1223
BsrI ACTGG 4 cut(s) 118, 152, 418, 1119
BssAI RCCGGY 3 cut(s) 179, 893, 1223
BssECI CCNNGG 4 cut(s) 522, 792, 1014, 1062
BssMI GATC 4 cut(s) 204, 433, 834, 1058
BssNI GRCGYC 3 cut(s) 299, 417, 549
BssSI CACGAG 2 cut(s) 197, 283
BssT1I CCWWGG 2 cut(s) 792, 1014
Bst2BI CACGAG 2 cut(s) 197, 283
Bst2UI CCWGG 1 cut(s) 802
Bst4CI ACNGT 8 cut(s) 488, 608, 883, 936, 984, 1157, 1436, 1491
Bst6I CTCTTC 1 cut(s) 1128
BstACI GRCGYC 3 cut(s) 299, 417, 549
BstBI TTCGAA 1 cut(s) 362
BstC8I GCNNGC 9 cut(s) 177, 181, 507, 602, 789, 1031, 1146, 1171, 1225
BstDEI CTNAG 4 cut(s) 20, 25, 371, 830
BstEII GGTNACC 1 cut(s) 608
BstF5I GGATG 2 cut(s) 613, 814
BstFNI CGCG 2 cut(s) 600, 850
BstH2I RGCGCY 4 cut(s) 410, 420, 530, 552
BstHHI GCGC 4 cut(s) 409, 419, 529, 551
BstKTI GATC 4 cut(s) 207, 436, 837, 1061
BstMBI GATC 4 cut(s) 204, 433, 834, 1058
BstMCI CGRYCG 2 cut(s) 898, 983
BstMWI GCNNNNNNNGC 4 cut(s) 231, 404, 698, 861
BstNI CCWGG 1 cut(s) 802
BstNSI RCATGY 2 cut(s) 661, 1033
BstPI GGTNACC 1 cut(s) 608
BstSCI CCNGG 3 cut(s) 800, 1062, 1242
BstSFI CTRYAG 1 cut(s) 70
BstSLI GKGCMC 1 cut(s) 684
BstUI CGCG 2 cut(s) 600, 850
BstV1I GCAGC 5 cut(s) 56, 84, 221, 848, 1319
BstX2I RGATCY 1 cut(s) 433
BstXI CCANNNNNNTGG 1 cut(s) 120
BstYI RGATCY 1 cut(s) 433
Bsu15I ATCGAT 1 cut(s) 1057
BsuTUI ATCGAT 1 cut(s) 1057
BtrI CACGTC 3 cut(s) 319, 628, 921
BtsCI GGATG 2 cut(s) 613, 814
BtsI GCAGTG 1 cut(s) 837
BtsIMutI CAGTG 3 cut(s) 75, 125, 837
BtuMI TCGCGA 1 cut(s) 850
Cac8I GCNNGC 9 cut(s) 177, 181, 507, 602, 789, 1031, 1146, 1171, 1225
CfoI GCGC 4 cut(s) 409, 419, 529, 551
Cfr10I RCCGGY 3 cut(s) 179, 893, 1223
ClaI ATCGAT 1 cut(s) 1057
CseI GACGC 1 cut(s) 264
Csp6I GTAC 2 cut(s) 124, 489
CspAI ACCGGT 1 cut(s) 893
CspCI CAANNNNNGTGG 2 cut(s) 907, 942
CviAII CATG 7 cut(s) 16, 496, 658, 722, 757, 1030, 1177
CviQI GTAC 2 cut(s) 124, 489
DdeI CTNAG 4 cut(s) 20, 25, 371, 830
DinI GGCGCC 2 cut(s) 418, 550
DpnI GATC 4 cut(s) 206, 435, 836, 1060
DpnII GATC 4 cut(s) 204, 433, 834, 1058
DraIII CACNNNGTG 1 cut(s) 742
DrdI GACNNNNNNGTC 1 cut(s) 731
DriI GACNNNNNGTC 3 cut(s) 151, 250, 897
DseDI GACNNNNNNGTC 1 cut(s) 731
EaeI YGGCCR 2 cut(s) 102, 690
Eam1104I CTCTTC 1 cut(s) 1128
Eam1105I GACNNNNNGTC 3 cut(s) 151, 250, 897
EarI CTCTTC 1 cut(s) 1128
EciI GGCGGA 1 cut(s) 416
Ecl136II GAGCTC 1 cut(s) 43
Eco130I CCWWGG 2 cut(s) 792, 1014
Eco147I AGGCCT 3 cut(s) 791, 1013, 1144
Eco24I GRGCYC 3 cut(s) 45, 1249, 1318
Eco31I GGTCTC 4 cut(s) 138, 254, 478, 884
Eco47I GGWCC 5 cut(s) 591, 733, 879, 1277, 1491
Eco53kI GAGCTC 1 cut(s) 43
Eco57I CTGAAG 2 cut(s) 515, 635
Eco88I CYCGRG 4 cut(s) 441, 1108, 1217, 1248
Eco91I GGTNACC 1 cut(s) 608
EcoICRI GAGCTC 1 cut(s) 43
EcoO109I RGGNCCY 2 cut(s) 591, 1277
EcoO65I GGTNACC 1 cut(s) 608
EcoRII CCWGG 1 cut(s) 800
EcoT14I CCWWGG 2 cut(s) 792, 1014
EcoT38I GRGCYC 3 cut(s) 45, 1249, 1318
EgeI GGCGCC 2 cut(s) 418, 550
EheI GGCGCC 2 cut(s) 418, 550
ErhI CCWWGG 2 cut(s) 792, 1014
Esp3I CGTCTC 3 cut(s) 222, 908, 1146
FaeI CATG 7 cut(s) 19, 499, 661, 725, 760, 1033, 1180
FaqI GGGAC 3 cut(s) 577, 892, 1290
FatI CATG 7 cut(s) 15, 495, 657, 721, 756, 1029, 1176
FauI CCCGC 2 cut(s) 848, 1178
FblI GTMKAC 3 cut(s) 144, 311, 1206
Fnu4HI GCNGC 9 cut(s) 70, 73, 235, 396, 399, 510, 636, 862, 1333
FokI GGATG 2 cut(s) 600, 821
FriOI GRGCYC 3 cut(s) 45, 1249, 1318
Fsp4HI GCNGC 9 cut(s) 70, 73, 235, 396, 399, 510, 636, 862, 1333
FspBI CTAG 1 cut(s) 542
GlaI GCGC 4 cut(s) 408, 418, 528, 550
GluI GCNGC 9 cut(s) 70, 73, 235, 396, 399, 510, 636, 862, 1333
GsaI CCCAGC 1 cut(s) 1293
GsuI CTGGAG 2 cut(s) 350, 1110
HaeII RGCGCY 4 cut(s) 410, 420, 530, 552
HapII CCGG 9 cut(s) 66, 137, 180, 552, 865, 894, 1064, 1224, 1243
HgaI GACGC 1 cut(s) 264
HhaI GCGC 4 cut(s) 409, 419, 529, 551
Hin1I GRCGYC 3 cut(s) 299, 417, 549
Hin1II CATG 7 cut(s) 19, 499, 661, 725, 760, 1033, 1180
Hin6I GCGC 4 cut(s) 407, 417, 527, 549
HinP1I GCGC 4 cut(s) 407, 417, 527, 549
HincII GTYRAC 3 cut(s) 145, 312, 932
HindII GTYRAC 3 cut(s) 145, 312, 932
HindIII AAGCTT 2 cut(s) 1384, 1409
HinfI GANTC 9 cut(s) 152, 209, 245, 359, 765, 962, 1067, 1181, 1211
HpaII CCGG 9 cut(s) 66, 137, 180, 552, 865, 894, 1064, 1224, 1243
HphI GGTGA 3 cut(s) 457, 602, 1512
Hpy166II GTNNAC 6 cut(s) 145, 312, 736, 745, 932, 1207
Hpy188I TCNGA 5 cut(s) 165, 259, 618, 967, 1534
Hpy188III TCNNGA 5 cut(s) 197, 653, 849, 1089, 1250
Hpy8I GTNNAC 6 cut(s) 145, 312, 736, 745, 932, 1207
Hpy99I CGWCG 8 cut(s) 256, 301, 304, 307, 316, 622, 625, 903
HpyAV CCTTC 5 cut(s) 329, 358, 539, 799, 1049
HpyCH4III ACNGT 8 cut(s) 488, 608, 883, 936, 984, 1157, 1436, 1491
HpyCH4IV ACGT 4 cut(s) 299, 318, 627, 920
HpyCH4V TGCA 5 cut(s) 72, 382, 787, 1029, 1237
HpyF10VI GCNNNNNNNGC 4 cut(s) 231, 404, 698, 861
HpyF3I CTNAG 4 cut(s) 20, 25, 371, 830
HpySE526I ACGT 4 cut(s) 299, 318, 627, 920
Hsp92I GRCGYC 3 cut(s) 299, 417, 549
Hsp92II CATG 7 cut(s) 19, 499, 661, 725, 760, 1033, 1180
HspAI GCGC 4 cut(s) 407, 417, 527, 549
KasI GGCGCC 2 cut(s) 416, 548
KflI GGGWCCC 2 cut(s) 591, 1277
KroI GCCGGC 2 cut(s) 179, 1223
KroNI GCCGGC 2 cut(s) 181, 1225
Kzo9I GATC 4 cut(s) 204, 433, 834, 1058
LmnI GCTCC 4 cut(s) 34, 166, 858, 1091
Lsp1109I GCAGC 5 cut(s) 56, 84, 221, 848, 1319
LweI GCATC 4 cut(s) 315, 353, 391, 774
MaeI CTAG 1 cut(s) 542
MaeII ACGT 4 cut(s) 299, 318, 627, 920
MaeIII GTNAC 5 cut(s) 9, 287, 319, 608, 1117
MalI GATC 4 cut(s) 206, 435, 836, 1060
MboI GATC 4 cut(s) 204, 433, 834, 1058
MboII GAAGA 2 cut(s) 1142, 1145
MflI RGATCY 1 cut(s) 433
MhlI GDGCHC 5 cut(s) 45, 606, 684, 1249, 1318
MluCI AATT 1 cut(s) 1253
Mly113I GGCGCC 2 cut(s) 417, 549
MlyI GAGTC 5 cut(s) 161, 218, 239, 1175, 1205
MmeI TCCRAC 3 cut(s) 641, 939, 1460
MreI CGCCGGCG 1 cut(s) 179
MroNI GCCGGC 2 cut(s) 179, 1223
MseI TTAA 3 cut(s) 1002, 1368, 1428
MspI CCGG 9 cut(s) 66, 137, 180, 552, 865, 894, 1064, 1224, 1243
MspR9I CCNGG 3 cut(s) 802, 1064, 1244
MvaI CCWGG 1 cut(s) 802
MvnI CGCG 2 cut(s) 600, 850
MwoI GCNNNNNNNGC 4 cut(s) 231, 404, 698, 861
NaeI GCCGGC 2 cut(s) 181, 1225
NarI GGCGCC 2 cut(s) 417, 549
NciI CCSGG 2 cut(s) 1064, 1244
NdeII GATC 4 cut(s) 204, 433, 834, 1058
NgoMIV GCCGGC 2 cut(s) 179, 1223
NlaIII CATG 7 cut(s) 19, 499, 661, 725, 760, 1033, 1180
NlaIV GGNNCC 8 cut(s) 418, 550, 592, 593, 734, 880, 1278, 1279
NmeAIII GCCGAG 2 cut(s) 251, 1178
NmuCI GTSAC 2 cut(s) 319, 608
NruI TCGCGA 1 cut(s) 850
NspI RCATGY 2 cut(s) 661, 1033
NspV TTCGAA 1 cut(s) 362
PaeI GCATGC 1 cut(s) 1033
PaeR7I CTCGAG 2 cut(s) 1217, 1248
PceI AGGCCT 3 cut(s) 791, 1013, 1144
PciI ACATGT 1 cut(s) 657
PcsI WCGNNNNNNNCGW 4 cut(s) 204, 308, 320, 1215
PdiI GCCGGC 2 cut(s) 181, 1225
PfeI GAWTC 4 cut(s) 359, 765, 962, 1067
PflFI GACNNNGTC 1 cut(s) 301
PflMI CCANNNNNTGG 1 cut(s) 742
PfoI TCCNGGA 1 cut(s) 800
PinAI ACCGGT 1 cut(s) 893
PkrI GCNGC 9 cut(s) 71, 74, 236, 397, 400, 511, 637, 863, 1334
PleI GAGTC 5 cut(s) 160, 217, 239, 1175, 1205
PluTI GGCGCC 2 cut(s) 420, 552
PpsI GAGTC 5 cut(s) 160, 217, 239, 1175, 1205
PpuMI RGGWCCY 2 cut(s) 591, 1277
PscI ACATGT 1 cut(s) 657
Psp124BI GAGCTC 1 cut(s) 45
Psp5II RGGWCCY 2 cut(s) 591, 1277
Psp6I CCWGG 1 cut(s) 800
PspEI GGTNACC 1 cut(s) 608
PspFI CCCAGC 1 cut(s) 1289
PspGI CCWGG 1 cut(s) 800
PspN4I GGNNCC 8 cut(s) 418, 550, 592, 593, 734, 880, 1278, 1279
PspPPI RGGWCCY 2 cut(s) 591, 1277
PspXI VCTCGAGB 1 cut(s) 1217
PstI CTGCAG 1 cut(s) 74
PsuI RGATCY 1 cut(s) 433
PsyI GACNNNGTC 1 cut(s) 301
RruI TCGCGA 1 cut(s) 850
RsaI GTAC 2 cut(s) 125, 490
RsaNI GTAC 2 cut(s) 124, 489
SacI GAGCTC 1 cut(s) 45
SalI GTCGAC 2 cut(s) 143, 310
SaqAI TTAA 3 cut(s) 1002, 1368, 1428
SatI GCNGC 9 cut(s) 70, 73, 235, 396, 399, 510, 636, 862, 1333
Sau3AI GATC 4 cut(s) 204, 433, 834, 1058
SchI GAGTC 5 cut(s) 161, 218, 239, 1175, 1205
ScrFI CCNGG 3 cut(s) 802, 1064, 1244
SduI GDGCHC 5 cut(s) 45, 606, 684, 1249, 1318
SfaNI GCATC 4 cut(s) 315, 353, 391, 774
SfcI CTRYAG 1 cut(s) 70
SfiI GGCCNNNNNGGCC 1 cut(s) 698
SfoI GGCGCC 2 cut(s) 418, 550
Sfr274I CTCGAG 2 cut(s) 1217, 1248
SfuI TTCGAA 1 cut(s) 362
SgrAI CRCCGGYG 1 cut(s) 179
SinI GGWCC 5 cut(s) 591, 733, 879, 1277, 1491
SlaI CTCGAG 2 cut(s) 1217, 1248
SmlI CTYRAG 5 cut(s) 38, 461, 1217, 1248, 1317
SmoI CTYRAG 5 cut(s) 38, 461, 1217, 1248, 1317
SphI GCATGC 1 cut(s) 1033
Sse9I AATT 1 cut(s) 1253
SseBI AGGCCT 3 cut(s) 791, 1013, 1144
SsiI CCGC 9 cut(s) 183, 395, 398, 401, 509, 598, 635, 855, 1171
SspDI GGCGCC 2 cut(s) 416, 548
SspMI CTAG 1 cut(s) 542
SstI GAGCTC 1 cut(s) 45
StuI AGGCCT 3 cut(s) 791, 1013, 1144
StyD4I CCNGG 3 cut(s) 800, 1062, 1242
StyI CCWWGG 2 cut(s) 792, 1014
TaaI ACNGT 8 cut(s) 488, 608, 883, 936, 984, 1157, 1436, 1491
TaiI ACGT 4 cut(s) 302, 321, 630, 923
TasI AATT 1 cut(s) 1253
TauI GCSGC 4 cut(s) 398, 401, 512, 638
TfiI GAWTC 4 cut(s) 359, 765, 962, 1067
Tru1I TTAA 3 cut(s) 1002, 1368, 1428
Tru9I TTAA 3 cut(s) 1002, 1368, 1428
TscAI CASTG 3 cut(s) 82, 125, 844
TseFI GTSAC 2 cut(s) 319, 608
TseI GCWGC 5 cut(s) 69, 72, 234, 861, 1332
Tsp45I GTSAC 2 cut(s) 319, 608
TspDTI ATGAA 2 cut(s) 935, 1165
TspGWI ACGGA 2 cut(s) 329, 638
TspRI CASTG 3 cut(s) 82, 125, 844
Tth111I GACNNNGTC 1 cut(s) 301
Van91I CCANNNNNTGG 1 cut(s) 742
VpaK11BI GGWCC 5 cut(s) 591, 733, 879, 1277, 1491
XceI RCATGY 2 cut(s) 661, 1033
XhoI CTCGAG 2 cut(s) 1217, 1248
XmiI GTMKAC 3 cut(s) 144, 311, 1206
XspI CTAG 1 cut(s) 542
ZraI GACGTC 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.