RchiOBHm_Chr5g0019731

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0018615
Physical Location & Seq
Forward (+)
14171520 .. 14172463
944 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 210 bp
ATGGGTGTAATACATACCGAATTGTCCAAGCCAAGCCGAAAGCAGATCATGACCTCCGGAGCTCCGCTGCCCGTGGCGGTTCTGTCTCGGATTGAGTCGCTGGGATTCGTGGTGAGCCATGAGTACGGTTTGACGGAGACGGCAGGGGTGGTGGTGTCGTGCGCGTGGACATGGTGGAGCTGCCATGATTGGTCTGAGACCAATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

69

Amino Acids

7.64

Weight (kDa)

5.79

Isoelectric Point (pI)

51.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 11 - 57 2.7e-06 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 164
AccIII TCCGGA 1 cut(s) 56
AciI CCGC 2 cut(s) 65, 77
AfaI GTAC 1 cut(s) 125
AluBI AGCT 2 cut(s) 62, 180
AluI AGCT 2 cut(s) 62, 180
Alw21I GWGCWC 1 cut(s) 64
Alw26I GTCTC 3 cut(s) 90, 131, 191
Aor13HI TCCGGA 1 cut(s) 56
ApeKI GCWGC 2 cut(s) 67, 180
AspLEI GCGC 1 cut(s) 164
AsuHPI GGTGA 1 cut(s) 124
BanII GRGCYC 1 cut(s) 64
Bbv12I GWGCWC 1 cut(s) 64
BbvI GCAGC 2 cut(s) 54, 167
BceAI ACGGC 1 cut(s) 156
BcoDI GTCTC 3 cut(s) 90, 131, 191
BisI GCNGC 2 cut(s) 68, 181
BlsI GCNGC 2 cut(s) 69, 182
BsaI GGTCTC 1 cut(s) 191
BsaJI CCNNGG 1 cut(s) 72
BsaWI WCCGGW 1 cut(s) 56
BseAI TCCGGA 1 cut(s) 56
BseDI CCNNGG 1 cut(s) 72
BseMII CTCAG 1 cut(s) 186
BseXI GCAGC 2 cut(s) 54, 167
BseYI CCCAGC 1 cut(s) 100
Bsh1236I CGCG 1 cut(s) 164
BsiHKAI GWGCWC 1 cut(s) 64
BsiSI CCGG 1 cut(s) 57
BsmAI GTCTC 3 cut(s) 90, 131, 191
BsmBI CGTCTC 1 cut(s) 131
Bso31I GGTCTC 1 cut(s) 191
Bsp1286I GDGCHC 1 cut(s) 64
Bsp13I TCCGGA 1 cut(s) 56
Bsp143I GATC 1 cut(s) 45
BspACI CCGC 2 cut(s) 65, 77
BspCNI CTCAG 1 cut(s) 187
BspEI TCCGGA 1 cut(s) 56
BspFNI CGCG 1 cut(s) 164
BspHI TCATGA 1 cut(s) 48
BspTNI GGTCTC 1 cut(s) 191
BssECI CCNNGG 1 cut(s) 72
BssMI GATC 1 cut(s) 45
Bst4CI ACNGT 1 cut(s) 128
BstDEI CTNAG 1 cut(s) 195
BstDSI CCRYGG 1 cut(s) 72
BstFNI CGCG 1 cut(s) 164
BstHHI GCGC 1 cut(s) 164
BstKTI GATC 1 cut(s) 48
BstMAI GTCTC 3 cut(s) 90, 131, 191
BstMBI GATC 1 cut(s) 45
BstUI CGCG 1 cut(s) 164
BstV1I GCAGC 2 cut(s) 54, 167
BtgI CCRYGG 1 cut(s) 72
CciI TCATGA 1 cut(s) 48
CfoI GCGC 1 cut(s) 164
Csp6I GTAC 1 cut(s) 124
CviAII CATG 4 cut(s) 49, 119, 171, 185
CviJI RGCY 5 cut(s) 31, 36, 62, 117, 180
CviKI_1 RGCY 5 cut(s) 31, 36, 62, 117, 180
CviQI GTAC 1 cut(s) 124
DdeI CTNAG 1 cut(s) 195
DpnI GATC 1 cut(s) 47
DpnII GATC 1 cut(s) 45
Ecl136II GAGCTC 1 cut(s) 62
Eco24I GRGCYC 1 cut(s) 64
Eco31I GGTCTC 1 cut(s) 191
Eco53kI GAGCTC 1 cut(s) 62
EcoICRI GAGCTC 1 cut(s) 62
EcoT38I GRGCYC 1 cut(s) 64
Esp3I CGTCTC 1 cut(s) 131
FaeI CATG 4 cut(s) 52, 122, 174, 188
FaiI YATR 5 cut(s) 15, 50, 120, 172, 186
FatI CATG 4 cut(s) 48, 118, 170, 184
Fnu4HI GCNGC 2 cut(s) 68, 181
FriOI GRGCYC 1 cut(s) 64
Fsp4HI GCNGC 2 cut(s) 68, 181
GlaI GCGC 1 cut(s) 163
GluI GCNGC 2 cut(s) 68, 181
GsaI CCCAGC 1 cut(s) 104
HapII CCGG 1 cut(s) 57
HhaI GCGC 1 cut(s) 164
Hin1II CATG 4 cut(s) 52, 122, 174, 188
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HinfI GANTC 2 cut(s) 95, 105
HpaII CCGG 1 cut(s) 57
HphI GGTGA 1 cut(s) 124
Hpy166II GTNNAC 1 cut(s) 168
Hpy188I TCNGA 2 cut(s) 90, 196
Hpy188III TCNNGA 2 cut(s) 49, 57
Hpy8I GTNNAC 1 cut(s) 168
HpyCH4III ACNGT 1 cut(s) 128
HpyF3I CTNAG 1 cut(s) 195
Hsp92II CATG 4 cut(s) 52, 122, 174, 188
HspAI GCGC 1 cut(s) 162
Kpn2I TCCGGA 1 cut(s) 56
Kzo9I GATC 1 cut(s) 45
LmnI GCTCC 3 cut(s) 59, 67, 177
LpnPI CCDG 3 cut(s) 70, 86, 129
Lsp1109I GCAGC 2 cut(s) 54, 167
MalI GATC 1 cut(s) 47
MboI GATC 1 cut(s) 45
MhlI GDGCHC 1 cut(s) 64
MluCI AATT 2 cut(s) 20, 202
MlyI GAGTC 1 cut(s) 104
MnlI CCTC 1 cut(s) 64
MroI TCCGGA 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 67
MspI CCGG 1 cut(s) 57
MvnI CGCG 1 cut(s) 164
NdeII GATC 1 cut(s) 45
NlaIII CATG 4 cut(s) 52, 122, 174, 188
PagI TCATGA 1 cut(s) 48
PfeI GAWTC 1 cut(s) 105
PkrI GCNGC 2 cut(s) 69, 182
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
Psp124BI GAGCTC 1 cut(s) 64
PspFI CCCAGC 1 cut(s) 100
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
SacI GAGCTC 1 cut(s) 64
SatI GCNGC 2 cut(s) 68, 181
Sau3AI GATC 1 cut(s) 45
SchI GAGTC 1 cut(s) 104
SduI GDGCHC 1 cut(s) 64
SetI ASST 3 cut(s) 56, 64, 182
Sse9I AATT 2 cut(s) 20, 202
SsiI CCGC 2 cut(s) 65, 77
SstI GAGCTC 1 cut(s) 64
TaaI ACNGT 1 cut(s) 128
TasI AATT 2 cut(s) 20, 202
TfiI GAWTC 1 cut(s) 105
TseI GCWGC 2 cut(s) 67, 180
TspGWI ACGGA 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.