Rmu_sc0000400.1_g000017

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000400.1
OG0018615
Physical Location & Seq
Reverse (-)
86595 .. 91443
4849 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000400.1_g000017.1.cds

Sequence Viewer

Length: 705 bp
atgaccgccggagctccactgcctgtggcagttctatctcggattgagtcgctaggattcgtggtgagccatgggtatgggttgacggagacggcgggggtggtggtgtcgtgcacgtggaagcccaggtggaataagttgccagcgcttagggatgaaagatggagatggagattaagaagagaaacaaaagtgatgagcacaccaggctccacttgcgcttcccattccttaggctcatcatcctccatcaacaccacaaagtcagtcctcccactcatcgacgtcggcgtcccttcgccgctcgaaagcgccttcaacctgctgtccatctgctttcagaggcgggtctgtgatgttggcggggaggaggttggccatgttggggtggattggcgcgtttcagtagctgaggatggcctcgctacccctgtagcacccgcaagccttgcttgccttctaccttcgggcgcgctcgcttgccctcgcgatcgctactcaccagcaaccccgcggcatctctgcagcgtcctcgtggcatctctgcagcgtcctcgcggcatctccgcagtgacaaggcacatgaatgatgcgctccaaagtgaatacctcaatgaggaagacccaagaaagctatgggttgaacttgagcagcgatttggcaacgttcgtgactccctgcttcctgatttagaagtgcgatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

234

Amino Acids

25.54

Weight (kDa)

8.2

Isoelectric Point (pI)

64.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 290
AatII GACGTC 1 cut(s) 288
Acc36I ACCTGC 1 cut(s) 330
AccBSI CCGCTC 1 cut(s) 304
AccII CGCG 5 cut(s) 399, 473, 489, 514, 558
AclI AACGTT 1 cut(s) 666
AcoI YGGCCR 1 cut(s) 376
AcvI CACGTG 1 cut(s) 117
AcyI GRCGYC 2 cut(s) 285, 291
AfeI AGCGCT 1 cut(s) 147
AfiI CCNNNNNNNGG 2 cut(s) 385, 616
AgsI TTSAA 2 cut(s) 319, 644
AjnI CCWGG 2 cut(s) 125, 205
AluBI AGCT 3 cut(s) 14, 410, 634
AluI AGCT 3 cut(s) 14, 410, 634
Alw21I GWGCWC 3 cut(s) 16, 116, 203
Alw26I GTCTC 1 cut(s) 83
Alw44I GTGCAC 1 cut(s) 112
AlwNI CAGNNNCTG 1 cut(s) 410
Aor51HI AGCGCT 1 cut(s) 147
AoxI GGCC 2 cut(s) 376, 418
ApaLI GTGCAC 1 cut(s) 112
ApeKI GCWGC 3 cut(s) 525, 547, 652
AsiSI GCGATCGC 1 cut(s) 493
AspLEI GCGC 7 cut(s) 148, 221, 314, 399, 473, 475, 595
AsuHPI GGTGA 2 cut(s) 76, 492
AxyI CCTNAGG 1 cut(s) 232
BaeGI GKGCMC 1 cut(s) 116
BalI TGGCCA 1 cut(s) 378
BanII GRGCYC 1 cut(s) 16
BauI CACGAG 1 cut(s) 533
BbrPI CACGTG 1 cut(s) 117
BbsI GAAGAC 1 cut(s) 627
Bbv12I GWGCWC 3 cut(s) 16, 116, 203
BbvCI CCTCAGC 1 cut(s) 411
BbvI GCAGC 3 cut(s) 537, 559, 664
BccI CCATC 5 cut(s) 156, 162, 257, 338, 410
BceAI ACGGC 1 cut(s) 108
BcgI CGANNNNNNTGC 4 cut(s) 514, 536, 548, 570
BciT130I CCWGG 2 cut(s) 127, 207
BcoDI GTCTC 1 cut(s) 83
BfaI CTAG 1 cut(s) 53
BfmI CTRYAG 3 cut(s) 432, 523, 545
BfoI RGCGCY 2 cut(s) 149, 315
BfuAI ACCTGC 1 cut(s) 330
BisI GCNGC 6 cut(s) 302, 515, 526, 548, 559, 653
BlsI GCNGC 6 cut(s) 303, 516, 527, 549, 560, 654
Bme1390I CCNGG 2 cut(s) 127, 207
BmiI GGNNCC 1 cut(s) 211
BmrFI CCNGG 2 cut(s) 127, 207
BmsI GCATC 4 cut(s) 526, 548, 570, 580
BpiI GAAGAC 1 cut(s) 627
Bpu10I CCTNAGC 2 cut(s) 149, 411
BpuEI CTTGAG 1 cut(s) 668
BsaAI YACGTR 1 cut(s) 117
BsaHI GRCGYC 2 cut(s) 285, 291
BsaJI CCNNGG 3 cut(s) 70, 125, 512
Bsc4I CCNNNNNNNGG 2 cut(s) 385, 616
Bse21I CCTNAGG 1 cut(s) 232
BseBI CCWGG 2 cut(s) 127, 207
BseDI CCNNGG 3 cut(s) 70, 125, 512
BseGI GGATG 3 cut(s) 160, 242, 421
BseLI CCNNNNNNNGG 2 cut(s) 385, 616
BseMII CTCAG 1 cut(s) 402
BsePI GCGCGC 1 cut(s) 471
BseRI GAGGAG 1 cut(s) 383
BseSI GKGCMC 1 cut(s) 116
BseXI GCAGC 3 cut(s) 537, 559, 664
Bsh1236I CGCG 5 cut(s) 399, 473, 489, 514, 558
Bsh1285I CGRYCG 1 cut(s) 493
BshFI GGCC 2 cut(s) 378, 420
BsiEI CGRYCG 1 cut(s) 493
BsiHKAI GWGCWC 3 cut(s) 16, 116, 203
BsiSI CCGG 1 cut(s) 9
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 2 cut(s) 385, 616
BsmAI GTCTC 1 cut(s) 83
BsmBI CGTCTC 1 cut(s) 83
BsmFI GGGAC 1 cut(s) 278
BsnI GGCC 2 cut(s) 378, 420
Bsp1286I GDGCHC 3 cut(s) 16, 116, 203
Bsp143I GATC 1 cut(s) 490
Bsp19I CCATGG 1 cut(s) 70
Bsp68I TCGCGA 1 cut(s) 489
BspANI GGCC 2 cut(s) 378, 420
BspCNI CTCAG 1 cut(s) 403
BspFNI CGCG 5 cut(s) 399, 473, 489, 514, 558
BspLI GGNNCC 1 cut(s) 211
BspMAI CTGCAG 2 cut(s) 527, 549
BspMI ACCTGC 1 cut(s) 330
BsrBI CCGCTC 1 cut(s) 304
BssECI CCNNGG 3 cut(s) 70, 125, 512
BssHII GCGCGC 1 cut(s) 471
BssMI GATC 1 cut(s) 490
BssNI GRCGYC 2 cut(s) 285, 291
BssSI CACGAG 1 cut(s) 533
BssT1I CCWWGG 1 cut(s) 70
Bst2BI CACGAG 1 cut(s) 533
Bst2UI CCWGG 2 cut(s) 127, 207
Bst6I CTCTTC 1 cut(s) 175
BstACI GRCGYC 2 cut(s) 285, 291
BstAPI GCANNNNNTGC 1 cut(s) 449
BstBAI YACGTR 1 cut(s) 117
BstC8I GCNNGC 6 cut(s) 144, 445, 454, 473, 477, 481
BstDEI CTNAG 3 cut(s) 149, 232, 411
BstDSI CCRYGG 2 cut(s) 70, 512
BstENI CCTNNNNNAGG 1 cut(s) 614
BstF5I GGATG 3 cut(s) 160, 242, 421
BstFNI CGCG 5 cut(s) 399, 473, 489, 514, 558
BstH2I RGCGCY 2 cut(s) 149, 315
BstHHI GCGC 7 cut(s) 148, 221, 314, 399, 473, 475, 595
BstKTI GATC 1 cut(s) 493
BstMAI GTCTC 1 cut(s) 83
BstMBI GATC 1 cut(s) 490
BstMCI CGRYCG 1 cut(s) 493
BstMWI GCNNNNNNNGC 4 cut(s) 207, 216, 449, 453
BstNI CCWGG 2 cut(s) 127, 207
BstSCI CCNGG 2 cut(s) 125, 205
BstSFI CTRYAG 3 cut(s) 432, 523, 545
BstSLI GKGCMC 1 cut(s) 116
BstUI CGCG 5 cut(s) 399, 473, 489, 514, 558
BstV1I GCAGC 3 cut(s) 537, 559, 664
BstV2I GAAGAC 1 cut(s) 627
BstXI CCANNNNNNTGG 1 cut(s) 77
Bsu36I CCTNAGG 1 cut(s) 232
BsuRI GGCC 2 cut(s) 378, 420
BtgI CCRYGG 2 cut(s) 70, 512
BtsCI GGATG 3 cut(s) 160, 242, 421
BtsI GCAGTG 2 cut(s) 17, 576
BtsIMutI CAGTG 2 cut(s) 17, 576
BtuMI TCGCGA 1 cut(s) 489
BveI ACCTGC 1 cut(s) 330
Cac8I GCNNGC 6 cut(s) 144, 445, 454, 473, 477, 481
CaiI CAGNNNCTG 1 cut(s) 410
CfoI GCGC 7 cut(s) 148, 221, 314, 399, 473, 475, 595
Cfr42I CCGCGG 1 cut(s) 515
CseI GACGC 3 cut(s) 280, 517, 539
CviAII CATG 3 cut(s) 71, 380, 583
DdeI CTNAG 3 cut(s) 149, 232, 411
DpnI GATC 1 cut(s) 492
DpnII GATC 1 cut(s) 490
DrdI GACNNNNNNGTC 1 cut(s) 290
DseDI GACNNNNNNGTC 1 cut(s) 290
EaeI YGGCCR 1 cut(s) 376
Eam1104I CTCTTC 1 cut(s) 175
EarI CTCTTC 1 cut(s) 175
Ecl136II GAGCTC 1 cut(s) 14
Eco130I CCWWGG 1 cut(s) 70
Eco24I GRGCYC 1 cut(s) 16
Eco47III AGCGCT 1 cut(s) 147
Eco53kI GAGCTC 1 cut(s) 14
Eco72I CACGTG 1 cut(s) 117
Eco81I CCTNAGG 1 cut(s) 232
EcoICRI GAGCTC 1 cut(s) 14
EcoNI CCTNNNNNAGG 1 cut(s) 614
EcoRII CCWGG 2 cut(s) 125, 205
EcoT14I CCWWGG 1 cut(s) 70
EcoT38I GRGCYC 1 cut(s) 16
ErhI CCWWGG 1 cut(s) 70
Esp3I CGTCTC 1 cut(s) 83
FaeI CATG 3 cut(s) 74, 383, 586
FaiI YATR 5 cut(s) 72, 78, 381, 584, 637
FalI AAGNNNNNCTT 2 cut(s) 436, 468
FaqI GGGAC 1 cut(s) 278
FatI CATG 3 cut(s) 70, 379, 582
FauI CCCGC 5 cut(s) 88, 339, 356, 448, 519
Fnu4HI GCNGC 6 cut(s) 302, 515, 526, 548, 559, 653
FokI GGATG 3 cut(s) 167, 229, 428
FriOI GRGCYC 1 cut(s) 16
Fsp4HI GCNGC 6 cut(s) 302, 515, 526, 548, 559, 653
FspBI CTAG 1 cut(s) 53
GlaI GCGC 7 cut(s) 147, 220, 313, 398, 472, 474, 594
GluI GCNGC 6 cut(s) 302, 515, 526, 548, 559, 653
HaeII RGCGCY 2 cut(s) 149, 315
HaeIII GGCC 2 cut(s) 378, 420
HapII CCGG 1 cut(s) 9
HgaI GACGC 3 cut(s) 280, 517, 539
HhaI GCGC 7 cut(s) 148, 221, 314, 399, 473, 475, 595
Hin1I GRCGYC 2 cut(s) 285, 291
Hin1II CATG 3 cut(s) 74, 383, 586
Hin6I GCGC 7 cut(s) 146, 219, 312, 397, 471, 473, 593
HinP1I GCGC 7 cut(s) 146, 219, 312, 397, 471, 473, 593
HincII GTYRAC 1 cut(s) 84
HindII GTYRAC 1 cut(s) 84
HinfI GANTC 3 cut(s) 47, 57, 674
HpaII CCGG 1 cut(s) 9
HphI GGTGA 2 cut(s) 76, 492
Hpy166II GTNNAC 2 cut(s) 84, 114
Hpy188I TCNGA 2 cut(s) 42, 342
Hpy188III TCNNGA 3 cut(s) 488, 671, 686
Hpy8I GTNNAC 2 cut(s) 84, 114
Hpy99I CGWCG 2 cut(s) 287, 290
HpyAV CCTTC 4 cut(s) 306, 325, 467, 474
HpyCH4IV ACGT 3 cut(s) 116, 285, 666
HpyCH4V TGCA 3 cut(s) 114, 525, 547
HpyF10VI GCNNNNNNNGC 4 cut(s) 207, 216, 449, 453
HpyF3I CTNAG 3 cut(s) 149, 232, 411
HpySE526I ACGT 3 cut(s) 116, 285, 666
Hsp92I GRCGYC 2 cut(s) 285, 291
Hsp92II CATG 3 cut(s) 74, 383, 586
HspAI GCGC 7 cut(s) 146, 219, 312, 397, 471, 473, 593
KspI CCGCGG 1 cut(s) 515
Kzo9I GATC 1 cut(s) 490
LmnI GCTCC 4 cut(s) 11, 19, 215, 600
Lsp1109I GCAGC 3 cut(s) 537, 559, 664
LweI GCATC 4 cut(s) 526, 548, 570, 580
MaeI CTAG 1 cut(s) 53
MaeII ACGT 3 cut(s) 116, 285, 666
MaeIII GTNAC 2 cut(s) 571, 671
MalI GATC 1 cut(s) 492
MbiI CCGCTC 1 cut(s) 304
MboI GATC 1 cut(s) 490
MboII GAAGA 2 cut(s) 192, 632
MhlI GDGCHC 3 cut(s) 16, 116, 203
MlsI TGGCCA 1 cut(s) 378
MluNI TGGCCA 1 cut(s) 378
MlyI GAGTC 2 cut(s) 56, 668
Mox20I TGGCCA 1 cut(s) 378
MscI TGGCCA 1 cut(s) 378
MseI TTAA 1 cut(s) 176
MslI CAYNNNNRTG 2 cut(s) 75, 585
Msp20I TGGCCA 1 cut(s) 378
MspA1I CMGCKG 1 cut(s) 514
MspI CCGG 1 cut(s) 9
MspR9I CCNGG 2 cut(s) 127, 207
MvaI CCWGG 2 cut(s) 127, 207
MvnI CGCG 5 cut(s) 399, 473, 489, 514, 558
MwoI GCNNNNNNNGC 4 cut(s) 207, 216, 449, 453
NcoI CCATGG 1 cut(s) 70
NdeII GATC 1 cut(s) 490
NlaIII CATG 3 cut(s) 74, 383, 586
NlaIV GGNNCC 1 cut(s) 211
NmuCI GTSAC 2 cut(s) 571, 671
NruI TCGCGA 1 cut(s) 489
PauI GCGCGC 1 cut(s) 471
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PfeI GAWTC 1 cut(s) 57
PkrI GCNGC 6 cut(s) 303, 516, 527, 549, 560, 654
Ple19I CGATCG 1 cut(s) 493
PleI GAGTC 2 cut(s) 55, 668
PmaCI CACGTG 1 cut(s) 117
PmlI CACGTG 1 cut(s) 117
PpsI GAGTC 2 cut(s) 55, 668
Ppu21I YACGTR 1 cut(s) 117
Psp124BI GAGCTC 1 cut(s) 16
Psp1406I AACGTT 1 cut(s) 666
Psp6I CCWGG 2 cut(s) 125, 205
PspCI CACGTG 1 cut(s) 117
PspGI CCWGG 2 cut(s) 125, 205
PspN4I GGNNCC 1 cut(s) 211
PstI CTGCAG 2 cut(s) 527, 549
PstNI CAGNNNCTG 1 cut(s) 410
PteI GCGCGC 1 cut(s) 471
PvuI CGATCG 1 cut(s) 493
RgaI GCGATCGC 1 cut(s) 493
RruI TCGCGA 1 cut(s) 489
RseI CAYNNNNRTG 2 cut(s) 75, 585
SacI GAGCTC 1 cut(s) 16
SacII CCGCGG 1 cut(s) 515
SaqAI TTAA 1 cut(s) 176
SatI GCNGC 6 cut(s) 302, 515, 526, 548, 559, 653
Sau3AI GATC 1 cut(s) 490
SchI GAGTC 2 cut(s) 56, 668
ScrFI CCNGG 2 cut(s) 127, 207
SduI GDGCHC 3 cut(s) 16, 116, 203
SfaAI GCGATCGC 1 cut(s) 493
SfaNI GCATC 4 cut(s) 526, 548, 570, 580
SfcI CTRYAG 3 cut(s) 432, 523, 545
Sfr303I CCGCGG 1 cut(s) 515
SgfI GCGATCGC 1 cut(s) 493
SgrBI CCGCGG 1 cut(s) 515
SmiMI CAYNNNNRTG 2 cut(s) 75, 585
SmlI CTYRAG 1 cut(s) 647
SmoI CTYRAG 1 cut(s) 647
SspMI CTAG 1 cut(s) 53
SstI GAGCTC 1 cut(s) 16
StyD4I CCNGG 2 cut(s) 125, 205
StyI CCWWGG 1 cut(s) 70
TaiI ACGT 3 cut(s) 119, 288, 669
TaqI TCGA 2 cut(s) 282, 306
TauI GCSGC 3 cut(s) 304, 517, 561
TfiI GAWTC 1 cut(s) 57
Tru1I TTAA 1 cut(s) 176
Tru9I TTAA 1 cut(s) 176
TscAI CASTG 2 cut(s) 24, 576
TseFI GTSAC 2 cut(s) 571, 671
TseI GCWGC 3 cut(s) 525, 547, 652
Tsp45I GTSAC 2 cut(s) 571, 671
TspDTI ATGAA 2 cut(s) 171, 599
TspGWI ACGGA 1 cut(s) 101
TspRI CASTG 2 cut(s) 24, 576
VneI GTGCAC 1 cut(s) 112
XagI CCTNNNNNAGG 1 cut(s) 614
XcmI CCANNNNNNNNNTGG 1 cut(s) 633
XspI CTAG 1 cut(s) 53
ZraI GACGTC 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.