RLG00000036224

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0018615
Physical Location & Seq
Forward (+)
77620649 .. 77622007
1359 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036224

Sequence Viewer

Length: 480 bp
ATGGAGTCATTCACGGGCTTTGGTCCGTCTCTTGCTTTTCTTCCTAGCCTCTCTGGTGGCTATGAGGCGAGCAGTGTTCTTGGGCGAGCGAGGGTGTTTGATGGTGAGCGCTGTAAAACAACCACTTACGGCGACTCGGGGATAGAGGAGTGCAAGGCTGATCATGGTCTTCGAAGGCATTCTGCGATGGTAATCTGGTTGATGGTGACGAATGCTGGGTATTCTCTTCGAGCCCGGCTGTGTGGGGATTGTGGAATCCTGGTGATTCTGGTTTCTGGTCTTGTTTATGCAGGTTGGTTCGATCTCTTTCTTCGGATTCTCCACGTCGATGGTGGACTGATCATGACCTCCGGAGCTCCGTTGCCCGTGAAAGTTCTATCTCGGATTGAGTCGTTGGGATTCGTGGTGAGCCGTGGGTACGAGTTGACTGAGACGGCGAGGGTGGGTGTCGTGTGTGTGGACGTGGTGGAGCTGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

160

Amino Acids

17.08

Weight (kDa)

5.89

Isoelectric Point (pI)

42.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 281
AccIII TCCGGA 1 cut(s) 350
AfaI GTAC 1 cut(s) 419
AfeI AGCGCT 1 cut(s) 110
AjiI CACGTC 2 cut(s) 325, 463
AjnI CCWGG 1 cut(s) 258
AluBI AGCT 2 cut(s) 356, 472
AluI AGCT 2 cut(s) 356, 472
Alw21I GWGCWC 1 cut(s) 358
Alw26I GTCTC 2 cut(s) 33, 425
Ama87I CYCGRG 1 cut(s) 136
Aor13HI TCCGGA 1 cut(s) 350
Aor51HI AGCGCT 1 cut(s) 110
ApeKI GCWGC 1 cut(s) 472
Asp700I GAANNNNTTC 1 cut(s) 178
AspLEI GCGC 1 cut(s) 111
AspS9I GGNCC 1 cut(s) 23
AsuC2I CCSGG 1 cut(s) 235
AsuHPI GGTGA 4 cut(s) 116, 217, 274, 418
AsuII TTCGAA 1 cut(s) 172
AvaI CYCGRG 1 cut(s) 136
AvaII GGWCC 1 cut(s) 23
BanII GRGCYC 2 cut(s) 235, 358
BbsI GAAGAC 1 cut(s) 161
Bbv12I GWGCWC 1 cut(s) 358
BbvI GCAGC 1 cut(s) 459
BccI CCATC 4 cut(s) 95, 181, 196, 323
BceAI ACGGC 3 cut(s) 145, 396, 450
BciT130I CCWGG 1 cut(s) 260
BclI TGATCA 2 cut(s) 160, 339
BcnI CCSGG 1 cut(s) 235
BcoDI GTCTC 2 cut(s) 33, 425
BfaI CTAG 1 cut(s) 45
BfoI RGCGCY 1 cut(s) 112
BfuAI ACCTGC 1 cut(s) 281
BisI GCNGC 1 cut(s) 473
BlsI GCNGC 1 cut(s) 474
Bme1390I CCNGG 2 cut(s) 235, 260
Bme18I GGWCC 1 cut(s) 23
BmeT110I CYCGRG 1 cut(s) 136
BmgBI CACGTC 2 cut(s) 325, 463
BmgT120I GGNCC 1 cut(s) 23
BmrFI CCNGG 2 cut(s) 235, 260
BpiI GAAGAC 1 cut(s) 161
Bpu14I TTCGAA 1 cut(s) 172
BpuMI CCSGG 1 cut(s) 235
BsaBI GATNNNNATC 1 cut(s) 191
BsaJI CCNNGG 1 cut(s) 412
BsaWI WCCGGW 1 cut(s) 350
Bse8I GATNNNNATC 1 cut(s) 191
BseAI TCCGGA 1 cut(s) 350
BseBI CCWGG 1 cut(s) 260
BseDI CCNNGG 1 cut(s) 412
BseJI GATNNNNATC 1 cut(s) 191
BseMII CTCAG 1 cut(s) 420
BseRI GAGGAG 1 cut(s) 161
BseXI GCAGC 1 cut(s) 459
BseYI CCCAGC 1 cut(s) 215
BsiHKAI GWGCWC 1 cut(s) 358
BsiHKCI CYCGRG 1 cut(s) 136
BsiSI CCGG 2 cut(s) 235, 351
BsmAI GTCTC 2 cut(s) 33, 425
BsmBI CGTCTC 2 cut(s) 33, 425
BsmI GAATGC 2 cut(s) 178, 217
BsoBI CYCGRG 1 cut(s) 136
Bsp119I TTCGAA 1 cut(s) 172
Bsp1286I GDGCHC 2 cut(s) 235, 358
Bsp13I TCCGGA 1 cut(s) 350
Bsp143I GATC 3 cut(s) 160, 301, 339
BspCNI CTCAG 1 cut(s) 421
BspEI TCCGGA 1 cut(s) 350
BspHI TCATGA 1 cut(s) 342
BspMI ACCTGC 1 cut(s) 281
BspT104I TTCGAA 1 cut(s) 172
BssECI CCNNGG 1 cut(s) 412
BssMI GATC 3 cut(s) 160, 301, 339
Bst2UI CCWGG 1 cut(s) 260
Bst6I CTCTTC 1 cut(s) 231
BstBI TTCGAA 1 cut(s) 172
BstC8I GCNNGC 2 cut(s) 70, 87
BstDEI CTNAG 1 cut(s) 429
BstDSI CCRYGG 1 cut(s) 412
BstH2I RGCGCY 1 cut(s) 112
BstHHI GCGC 1 cut(s) 111
BstKTI GATC 3 cut(s) 163, 304, 342
BstMAI GTCTC 2 cut(s) 33, 425
BstMBI GATC 3 cut(s) 160, 301, 339
BstNI CCWGG 1 cut(s) 260
BstSCI CCNGG 2 cut(s) 233, 258
BstV1I GCAGC 1 cut(s) 459
BstV2I GAAGAC 1 cut(s) 161
BstXI CCANNNNNNTGG 1 cut(s) 329
BtgI CCRYGG 1 cut(s) 412
BtgZI GCGATG 1 cut(s) 200
BtrI CACGTC 2 cut(s) 325, 463
BtsI GCAGTG 1 cut(s) 79
BtsIMutI CAGTG 1 cut(s) 79
BveI ACCTGC 1 cut(s) 281
Cac8I GCNNGC 2 cut(s) 70, 87
CciI TCATGA 1 cut(s) 342
CfoI GCGC 1 cut(s) 111
Cfr13I GGNCC 1 cut(s) 23
Csp6I GTAC 1 cut(s) 418
CviAII CATG 3 cut(s) 164, 343, 477
CviJI RGCY 9 cut(s) 18, 48, 60, 158, 233, 238, 356, 411, 472
CviKI_1 RGCY 9 cut(s) 18, 48, 60, 158, 233, 238, 356, 411, 472
CviQI GTAC 1 cut(s) 418
DdeI CTNAG 1 cut(s) 429
DpnI GATC 3 cut(s) 162, 303, 341
DpnII GATC 3 cut(s) 160, 301, 339
Eam1104I CTCTTC 1 cut(s) 231
EarI CTCTTC 1 cut(s) 231
Ecl136II GAGCTC 1 cut(s) 356
Eco24I GRGCYC 2 cut(s) 235, 358
Eco47I GGWCC 1 cut(s) 23
Eco47III AGCGCT 1 cut(s) 110
Eco53kI GAGCTC 1 cut(s) 356
Eco88I CYCGRG 1 cut(s) 136
EcoICRI GAGCTC 1 cut(s) 356
EcoRII CCWGG 1 cut(s) 258
EcoT38I GRGCYC 2 cut(s) 235, 358
Esp3I CGTCTC 2 cut(s) 33, 425
FaeI CATG 3 cut(s) 167, 346, 480
FaiI YATR 5 cut(s) 63, 165, 288, 344, 478
FatI CATG 3 cut(s) 163, 342, 476
FbaI TGATCA 2 cut(s) 160, 339
Fnu4HI GCNGC 1 cut(s) 473
FriOI GRGCYC 2 cut(s) 235, 358
Fsp4HI GCNGC 1 cut(s) 473
FspBI CTAG 1 cut(s) 45
GlaI GCGC 1 cut(s) 110
GluI GCNGC 1 cut(s) 473
GsaI CCCAGC 1 cut(s) 219
HaeII RGCGCY 1 cut(s) 112
HapII CCGG 2 cut(s) 235, 351
HhaI GCGC 1 cut(s) 111
Hin1II CATG 3 cut(s) 167, 346, 480
Hin6I GCGC 1 cut(s) 109
HinP1I GCGC 1 cut(s) 109
HincII GTYRAC 1 cut(s) 426
HindII GTYRAC 1 cut(s) 426
HinfI GANTC 7 cut(s) 5, 134, 255, 265, 316, 389, 399
HpaII CCGG 2 cut(s) 235, 351
HphI GGTGA 4 cut(s) 116, 217, 274, 418
Hpy166II GTNNAC 3 cut(s) 335, 426, 460
Hpy188I TCNGA 2 cut(s) 315, 384
Hpy188III TCNNGA 2 cut(s) 343, 351
Hpy8I GTNNAC 3 cut(s) 335, 426, 460
Hpy99I CGWCG 1 cut(s) 329
HpyAV CCTTC 1 cut(s) 168
HpyCH4IV ACGT 2 cut(s) 324, 462
HpyCH4V TGCA 2 cut(s) 153, 290
HpyF3I CTNAG 1 cut(s) 429
HpySE526I ACGT 2 cut(s) 324, 462
Hsp92II CATG 3 cut(s) 167, 346, 480
HspAI GCGC 1 cut(s) 109
Kpn2I TCCGGA 1 cut(s) 350
Ksp22I TGATCA 2 cut(s) 160, 339
Kzo9I GATC 3 cut(s) 160, 301, 339
LmnI GCTCC 3 cut(s) 353, 361, 469
Lsp1109I GCAGC 1 cut(s) 459
MaeI CTAG 1 cut(s) 45
MaeII ACGT 2 cut(s) 324, 462
MaeIII GTNAC 1 cut(s) 205
MalI GATC 3 cut(s) 162, 303, 341
MboI GATC 3 cut(s) 160, 301, 339
MboII GAAGA 4 cut(s) 32, 161, 218, 302
MhlI GDGCHC 2 cut(s) 235, 358
MlyI GAGTC 3 cut(s) 14, 128, 398
MnlI CCTC 6 cut(s) 58, 59, 84, 139, 358, 432
MroI TCCGGA 1 cut(s) 350
MroXI GAANNNNTTC 1 cut(s) 178
MslI CAYNNNNRTG 1 cut(s) 327
MspI CCGG 2 cut(s) 235, 351
MspR9I CCNGG 2 cut(s) 235, 260
Mva1269I GAATGC 2 cut(s) 178, 217
MvaI CCWGG 1 cut(s) 260
NciI CCSGG 1 cut(s) 235
NdeII GATC 3 cut(s) 160, 301, 339
NlaIII CATG 3 cut(s) 167, 346, 480
NmuCI GTSAC 1 cut(s) 205
NspV TTCGAA 1 cut(s) 172
PagI TCATGA 1 cut(s) 342
PctI GAATGC 2 cut(s) 178, 217
PdmI GAANNNNTTC 1 cut(s) 178
PfeI GAWTC 4 cut(s) 255, 265, 316, 399
PkrI GCNGC 1 cut(s) 474
PleI GAGTC 3 cut(s) 13, 128, 397
PpsI GAGTC 3 cut(s) 13, 128, 397
Psp124BI GAGCTC 1 cut(s) 358
Psp6I CCWGG 1 cut(s) 258
PspFI CCCAGC 1 cut(s) 215
PspGI CCWGG 1 cut(s) 258
PspPI GGNCC 1 cut(s) 23
RsaI GTAC 1 cut(s) 419
RsaNI GTAC 1 cut(s) 418
RseI CAYNNNNRTG 1 cut(s) 327
SacI GAGCTC 1 cut(s) 358
SatI GCNGC 1 cut(s) 473
Sau3AI GATC 3 cut(s) 160, 301, 339
Sau96I GGNCC 1 cut(s) 23
SchI GAGTC 3 cut(s) 14, 128, 398
ScrFI CCNGG 2 cut(s) 235, 260
SduI GDGCHC 2 cut(s) 235, 358
SetI ASST 6 cut(s) 295, 327, 350, 358, 465, 474
SfuI TTCGAA 1 cut(s) 172
SinI GGWCC 1 cut(s) 23
SmiMI CAYNNNNRTG 1 cut(s) 327
SspMI CTAG 1 cut(s) 45
SstI GAGCTC 1 cut(s) 358
StyD4I CCNGG 2 cut(s) 233, 258
TaiI ACGT 2 cut(s) 327, 465
TaqI TCGA 4 cut(s) 172, 229, 300, 327
TfiI GAWTC 4 cut(s) 255, 265, 316, 399
TscAI CASTG 1 cut(s) 79
TseFI GTSAC 1 cut(s) 205
TseI GCWGC 1 cut(s) 472
Tsp45I GTSAC 1 cut(s) 205
TspGWI ACGGA 2 cut(s) 15, 348
TspRI CASTG 1 cut(s) 79
VpaK11BI GGWCC 1 cut(s) 23
XcmI CCANNNNNNNNNTGG 1 cut(s) 329
XmnI GAANNNNTTC 1 cut(s) 178
XspI CTAG 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.