Rmu_sc0022726.1_g000009

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0022726.1
OG0018615
Physical Location & Seq
Forward (+)
34281 .. 34604
324 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0022726.1_g000009.1.cds

Sequence Viewer

Length: 324 bp
atgggtcaaaagcatatcgtattgaccgaatcgagcagcaaccagatcatgactgttgaagctccgctgcccatggcagttctgtctcggattgagtcgctgggattcgtggtgagccatgggtacgggttgactgagacgacgagggtggtggtgtcgtgcgtgtggaagcccgggtggaataagttgccagcgacagaaagggcaaggctgaagttgaggcagggagtgaggacggtggccatgaccggggtggacgtgatcgatccggatacgggtaaactggttaaacgcgacggttcgtcaaccgtcgcttggggataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

107

Amino Acids

11.61

Weight (kDa)

9.65

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 294
AccIII TCCGGA 1 cut(s) 268
AciI CCGC 1 cut(s) 65
AclWI GGATC 1 cut(s) 260
AcoI YGGCCR 1 cut(s) 240
AcuI CTGAAG 1 cut(s) 233
AfaI GTAC 1 cut(s) 125
AfiI CCNNNNNNNGG 3 cut(s) 249, 275, 315
AgsI TTSAA 1 cut(s) 59
AhdI GACNNNNNGTC 1 cut(s) 301
AjiI CACGTC 1 cut(s) 259
AluBI AGCT 1 cut(s) 62
AluI AGCT 1 cut(s) 62
Alw26I GTCTC 2 cut(s) 90, 131
AlwI GGATC 1 cut(s) 260
Ama87I CYCGRG 1 cut(s) 173
Aor13HI TCCGGA 1 cut(s) 268
AoxI GGCC 1 cut(s) 240
ApeKI GCWGC 2 cut(s) 36, 67
AsuC2I CCSGG 3 cut(s) 174, 175, 250
AsuHPI GGTGA 1 cut(s) 124
AvaI CYCGRG 1 cut(s) 173
BalI TGGCCA 1 cut(s) 242
BbvI GCAGC 2 cut(s) 48, 54
BciVI GTATCC 1 cut(s) 265
BcnI CCSGG 3 cut(s) 174, 175, 250
BcoDI GTCTC 2 cut(s) 90, 131
BfuI GTATCC 1 cut(s) 265
BisI GCNGC 2 cut(s) 37, 68
BlsI GCNGC 2 cut(s) 38, 69
Bme1390I CCNGG 3 cut(s) 174, 175, 250
BmeRI GACNNNNNGTC 1 cut(s) 301
BmeT110I CYCGRG 1 cut(s) 173
BmgBI CACGTC 1 cut(s) 259
BmrFI CCNGG 3 cut(s) 174, 175, 250
BpuMI CCSGG 3 cut(s) 174, 175, 250
Bsa29I ATCGAT 1 cut(s) 264
BsaJI CCNNGG 4 cut(s) 72, 118, 173, 249
BsaWI WCCGGW 1 cut(s) 268
Bsc4I CCNNNNNNNGG 3 cut(s) 249, 275, 315
Bse1I ACTGG 1 cut(s) 288
BseAI TCCGGA 1 cut(s) 268
BseCI ATCGAT 1 cut(s) 264
BseDI CCNNGG 4 cut(s) 72, 118, 173, 249
BseLI CCNNNNNNNGG 3 cut(s) 249, 275, 315
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 1 cut(s) 288
BseXI GCAGC 2 cut(s) 48, 54
BseYI CCCAGC 1 cut(s) 100
Bsh1236I CGCG 1 cut(s) 294
BshFI GGCC 1 cut(s) 242
BshVI ATCGAT 1 cut(s) 264
BsiHKCI CYCGRG 1 cut(s) 173
BsiSI CCGG 3 cut(s) 174, 249, 269
BslI CCNNNNNNNGG 3 cut(s) 249, 275, 315
BsmAI GTCTC 2 cut(s) 90, 131
BsmBI CGTCTC 1 cut(s) 131
BsnI GGCC 1 cut(s) 242
BsoBI CYCGRG 1 cut(s) 173
Bsp13I TCCGGA 1 cut(s) 268
Bsp143I GATC 3 cut(s) 45, 261, 265
Bsp19I CCATGG 2 cut(s) 72, 118
BspACI CCGC 1 cut(s) 65
BspANI GGCC 1 cut(s) 242
BspCNI CTCAG 1 cut(s) 127
BspDI ATCGAT 1 cut(s) 264
BspEI TCCGGA 1 cut(s) 268
BspFNI CGCG 1 cut(s) 294
BspHI TCATGA 1 cut(s) 48
BspPI GGATC 1 cut(s) 260
BsrI ACTGG 1 cut(s) 288
BssECI CCNNGG 4 cut(s) 72, 118, 173, 249
BssMI GATC 3 cut(s) 45, 261, 265
BssT1I CCWWGG 2 cut(s) 72, 118
Bst4CI ACNGT 4 cut(s) 55, 238, 299, 310
BstC8I GCNNGC 1 cut(s) 192
BstDEI CTNAG 1 cut(s) 135
BstDSI CCRYGG 2 cut(s) 72, 118
BstFNI CGCG 1 cut(s) 294
BstKTI GATC 3 cut(s) 48, 264, 268
BstMAI GTCTC 2 cut(s) 90, 131
BstMBI GATC 3 cut(s) 45, 261, 265
BstSCI CCNGG 3 cut(s) 172, 173, 248
BstUI CGCG 1 cut(s) 294
BstV1I GCAGC 2 cut(s) 48, 54
Bsu15I ATCGAT 1 cut(s) 264
BsuI GTATCC 1 cut(s) 265
BsuRI GGCC 1 cut(s) 242
BsuTUI ATCGAT 1 cut(s) 264
BtgI CCRYGG 2 cut(s) 72, 118
BtrI CACGTC 1 cut(s) 259
Cac8I GCNNGC 1 cut(s) 192
CciI TCATGA 1 cut(s) 48
Cfr9I CCCGGG 1 cut(s) 173
ClaI ATCGAT 1 cut(s) 264
Csp6I GTAC 1 cut(s) 124
CviAII CATG 4 cut(s) 49, 73, 119, 244
CviJI RGCY 5 cut(s) 62, 117, 172, 211, 242
CviKI_1 RGCY 5 cut(s) 62, 117, 172, 211, 242
CviQI GTAC 1 cut(s) 124
DdeI CTNAG 1 cut(s) 135
DpnI GATC 3 cut(s) 47, 263, 267
DpnII GATC 3 cut(s) 45, 261, 265
DriI GACNNNNNGTC 1 cut(s) 301
EaeI YGGCCR 1 cut(s) 240
Eam1105I GACNNNNNGTC 1 cut(s) 301
Eco130I CCWWGG 2 cut(s) 72, 118
Eco57I CTGAAG 1 cut(s) 233
Eco88I CYCGRG 1 cut(s) 173
EcoT14I CCWWGG 2 cut(s) 72, 118
ErhI CCWWGG 2 cut(s) 72, 118
Esp3I CGTCTC 1 cut(s) 131
FaeI CATG 4 cut(s) 52, 76, 122, 247
FaiI YATR 5 cut(s) 15, 50, 74, 120, 245
FatI CATG 4 cut(s) 48, 72, 118, 243
Fnu4HI GCNGC 2 cut(s) 37, 68
Fsp4HI GCNGC 2 cut(s) 37, 68
GluI GCNGC 2 cut(s) 37, 68
GsaI CCCAGC 1 cut(s) 104
HaeIII GGCC 1 cut(s) 242
HapII CCGG 3 cut(s) 174, 249, 269
Hin1II CATG 4 cut(s) 52, 76, 122, 247
HincII GTYRAC 2 cut(s) 132, 306
HindII GTYRAC 2 cut(s) 132, 306
HinfI GANTC 3 cut(s) 29, 95, 105
HpaII CCGG 3 cut(s) 174, 249, 269
HphI GGTGA 1 cut(s) 124
Hpy166II GTNNAC 4 cut(s) 132, 256, 281, 306
Hpy188I TCNGA 1 cut(s) 90
Hpy188III TCNNGA 2 cut(s) 49, 269
Hpy8I GTNNAC 4 cut(s) 132, 256, 281, 306
Hpy99I CGWCG 3 cut(s) 145, 299, 314
HpyCH4III ACNGT 4 cut(s) 55, 238, 299, 310
HpyCH4IV ACGT 1 cut(s) 258
HpyF3I CTNAG 1 cut(s) 135
HpySE526I ACGT 1 cut(s) 258
Hsp92II CATG 4 cut(s) 52, 76, 122, 247
Kpn2I TCCGGA 1 cut(s) 268
Kzo9I GATC 3 cut(s) 45, 261, 265
LmnI GCTCC 1 cut(s) 67
LpnPI CCDG 8 cut(s) 56, 86, 187, 204, 209, 262, 269, 282
Lsp1109I GCAGC 2 cut(s) 48, 54
MaeII ACGT 1 cut(s) 258
MalI GATC 3 cut(s) 47, 263, 267
MboI GATC 3 cut(s) 45, 261, 265
MlsI TGGCCA 1 cut(s) 242
MluNI TGGCCA 1 cut(s) 242
MlyI GAGTC 1 cut(s) 104
MnlI CCTC 3 cut(s) 138, 213, 225
Mox20I TGGCCA 1 cut(s) 242
MroI TCCGGA 1 cut(s) 268
MscI TGGCCA 1 cut(s) 242
MseI TTAA 1 cut(s) 288
Msp20I TGGCCA 1 cut(s) 242
MspA1I CMGCKG 1 cut(s) 67
MspI CCGG 3 cut(s) 174, 249, 269
MspR9I CCNGG 3 cut(s) 174, 175, 250
MvnI CGCG 1 cut(s) 294
NciI CCSGG 3 cut(s) 174, 175, 250
NcoI CCATGG 2 cut(s) 72, 118
NdeII GATC 3 cut(s) 45, 261, 265
NlaIII CATG 4 cut(s) 52, 76, 122, 247
PagI TCATGA 1 cut(s) 48
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PfeI GAWTC 2 cut(s) 29, 105
PkrI GCNGC 2 cut(s) 38, 69
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
PspFI CCCAGC 1 cut(s) 100
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
SaqAI TTAA 1 cut(s) 288
SatI GCNGC 2 cut(s) 37, 68
Sau3AI GATC 3 cut(s) 45, 261, 265
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 3 cut(s) 174, 175, 250
SetI ASST 2 cut(s) 64, 261
SmaI CCCGGG 1 cut(s) 175
SsiI CCGC 1 cut(s) 65
StyD4I CCNGG 3 cut(s) 172, 173, 248
StyI CCWWGG 2 cut(s) 72, 118
TaaI ACNGT 4 cut(s) 55, 238, 299, 310
TaiI ACGT 1 cut(s) 261
TaqI TCGA 2 cut(s) 32, 264
TaqII GACCGA 1 cut(s) 41
TfiI GAWTC 2 cut(s) 29, 105
Tru1I TTAA 1 cut(s) 288
Tru9I TTAA 1 cut(s) 288
TseI GCWGC 2 cut(s) 36, 67
TspMI CCCGGG 1 cut(s) 173
XcmI CCANNNNNNNNNTGG 1 cut(s) 250
XmaI CCCGGG 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.