RchiOBHm_Chr5g0024731

Thiosulfate 3-mercaptopyruvate sulfurtransferase 1, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0008731
Physical Location & Seq
Reverse (-)
18802105 .. 18813637
11533 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 693 bp
ATGCAGAAAAGATCAGATCTCTTGCGAGCTGACTCTTCCTATACAAAATATAGAGTATCTGGTTTAATACCTCGTGCTATGGCTTCATCAGTAGCTGGGGCAACAGCAGATTTTTTAACCTCGGCATCTACAAATGAGCCTGTTGTTTCTGTTGATTGGCTCCATGCTAATCTCAAGGAGCCTGACCTTAAGGTTGTGGATGCGTCTTGGTACATGCCTGATGAGCAAAGGAATCCGATTCAAGAGTATCAGGTTGCTCATATTCCTGGTGCCCTTTTCTTTGACGTAGATGGAATATCGGATCGCACAACAAAATTACCACTTATGTTACCATCTGAGGAGACCTTTGCTGCTGCTGTTTCTGCTCTTGGCATTGAGAACAAAGACGATCTAGTTGTGTATGATGGGAAAGGGCTTTTTAGTGCAGCTTATGGAGGCTTGCCAAGATGGCGTGCATCTGGATATGACGTTGAATCTAGTGCTTCTGGCAATGCGATTTTGAAAGCTAGTGCTGCCAGTGAGGCAATGGAGAAAGTATATAAGGGACAGCCAGTTGGTCCAACCACATTTCAGACTAAATTTCAGCCACATCTTGCCTGGAATCTTGAGCAGGATTTTGGAGCTCATAGAAGATGGATGACCTCAAAGAGCGGCTGCTTGCACCAAAAGTTCCAAAACCTGCATCTCCTATAA

Protein Analysis

230

Amino Acids

25.3

Weight (kDa)

6.07

Isoelectric Point (pI)

42.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 50 - 144 5.1e-06 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 687
AccB1I GGYRCC 1 cut(s) 269
AccBSI CCGCTC 1 cut(s) 651
AciI CCGC 1 cut(s) 651
AclWI GGATC 1 cut(s) 309
AcsI RAATTY 1 cut(s) 578
AfaI GTAC 1 cut(s) 212
AflII CTTAAG 1 cut(s) 188
AgsI TTSAA 3 cut(s) 242, 473, 502
AjnI CCWGG 2 cut(s) 265, 596
AluBI AGCT 5 cut(s) 29, 95, 428, 506, 623
AluI AGCT 5 cut(s) 29, 95, 428, 506, 623
Alw21I GWGCWC 1 cut(s) 625
Alw26I GTCTC 1 cut(s) 335
AlwI GGATC 1 cut(s) 309
AlwNI CAGNNNCTG 1 cut(s) 95
ApeKI GCWGC 5 cut(s) 350, 353, 425, 512, 654
ApoI RAATTY 1 cut(s) 578
AspS9I GGNCC 1 cut(s) 557
AvaII GGWCC 1 cut(s) 557
BaeGI GKGCMC 1 cut(s) 274
BanI GGYRCC 1 cut(s) 269
BanII GRGCYC 1 cut(s) 625
BauI CACGAG 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 625
BbvI GCAGC 5 cut(s) 337, 340, 437, 499, 641
BccI CCATC 5 cut(s) 284, 340, 398, 441, 627
BciT130I CCWGG 2 cut(s) 267, 598
BcoDI GTCTC 1 cut(s) 335
BfaI CTAG 3 cut(s) 392, 477, 507
BfrI CTTAAG 1 cut(s) 188
BfuAI ACCTGC 1 cut(s) 687
BglI GCCNNNNNGGC 2 cut(s) 448, 521
BglII AGATCT 1 cut(s) 16
BisI GCNGC 6 cut(s) 351, 354, 426, 513, 652, 655
BlsI GCNGC 6 cut(s) 352, 355, 427, 514, 653, 656
Bme1390I CCNGG 2 cut(s) 267, 598
Bme18I GGWCC 1 cut(s) 557
BmgT120I GGNCC 1 cut(s) 557
BmiI GGNNCC 3 cut(s) 161, 180, 271
BmrFI CCNGG 2 cut(s) 267, 598
BmsI GCATC 3 cut(s) 134, 190, 464
BpuEI CTTGAG 2 cut(s) 158, 626
BsaI GGTCTC 1 cut(s) 335
BsaJI CCNNGG 1 cut(s) 120
Bse1I ACTGG 2 cut(s) 516, 551
Bse3DI GCAATG 2 cut(s) 496, 531
BseBI CCWGG 2 cut(s) 267, 598
BseDI CCNNGG 1 cut(s) 120
BseGI GGATG 2 cut(s) 205, 642
BseMI GCAATG 2 cut(s) 496, 531
BseMII CTCAG 1 cut(s) 327
BseNI ACTGG 2 cut(s) 516, 551
BseRI GAGGAG 1 cut(s) 353
BseSI GKGCMC 1 cut(s) 274
BseXI GCAGC 5 cut(s) 337, 340, 437, 499, 641
BseYI CCCAGC 1 cut(s) 95
BsgI GTGCAG 1 cut(s) 444
BshNI GGYRCC 1 cut(s) 269
BsiHKAI GWGCWC 1 cut(s) 625
BslFI GGGAC 1 cut(s) 558
BsmAI GTCTC 1 cut(s) 335
BsmFI GGGAC 1 cut(s) 558
Bso31I GGTCTC 1 cut(s) 335
Bsp1286I GDGCHC 2 cut(s) 274, 625
Bsp143I GATC 4 cut(s) 11, 16, 301, 388
BspACI CCGC 1 cut(s) 651
BspCNI CTCAG 1 cut(s) 328
BspLI GGNNCC 3 cut(s) 161, 180, 271
BspMI ACCTGC 1 cut(s) 687
BspPI GGATC 1 cut(s) 309
BspT107I GGYRCC 1 cut(s) 269
BspTI CTTAAG 1 cut(s) 188
BspTNI GGTCTC 1 cut(s) 335
BsrBI CCGCTC 1 cut(s) 651
BsrDI GCAATG 2 cut(s) 496, 531
BsrI ACTGG 2 cut(s) 516, 551
BssECI CCNNGG 1 cut(s) 120
BssMI GATC 4 cut(s) 11, 16, 301, 388
BssSI CACGAG 1 cut(s) 72
Bst2BI CACGAG 1 cut(s) 72
Bst2UI CCWGG 2 cut(s) 267, 598
Bst6I CTCTTC 1 cut(s) 40
BstAFI CTTAAG 1 cut(s) 188
BstC8I GCNNGC 4 cut(s) 27, 440, 453, 659
BstDEI CTNAG 1 cut(s) 336
BstF5I GGATG 2 cut(s) 205, 642
BstKTI GATC 4 cut(s) 14, 19, 304, 391
BstMAI GTCTC 1 cut(s) 335
BstMBI GATC 4 cut(s) 11, 16, 301, 388
BstMWI GCNNNNNNNGC 5 cut(s) 223, 362, 448, 512, 521
BstNI CCWGG 2 cut(s) 267, 598
BstNSI RCATGY 1 cut(s) 217
BstSCI CCNGG 2 cut(s) 265, 596
BstSLI GKGCMC 1 cut(s) 274
BstV1I GCAGC 5 cut(s) 337, 340, 437, 499, 641
BstX2I RGATCY 1 cut(s) 16
BstYI RGATCY 1 cut(s) 16
BtsCI GGATG 2 cut(s) 205, 642
BtsIMutI CAGTG 1 cut(s) 523
BveI ACCTGC 1 cut(s) 687
Cac8I GCNNGC 4 cut(s) 27, 440, 453, 659
CaiI CAGNNNCTG 1 cut(s) 95
Cfr13I GGNCC 1 cut(s) 557
CseI GACGC 1 cut(s) 192
Csp6I GTAC 1 cut(s) 211
CviAII CATG 2 cut(s) 164, 214
CviQI GTAC 1 cut(s) 211
DdeI CTNAG 1 cut(s) 336
DpnI GATC 4 cut(s) 13, 18, 303, 390
DpnII GATC 4 cut(s) 11, 16, 301, 388
Eam1104I CTCTTC 1 cut(s) 40
EarI CTCTTC 1 cut(s) 40
Ecl136II GAGCTC 1 cut(s) 623
Eco24I GRGCYC 1 cut(s) 625
Eco31I GGTCTC 1 cut(s) 335
Eco47I GGWCC 1 cut(s) 557
Eco53kI GAGCTC 1 cut(s) 623
EcoICRI GAGCTC 1 cut(s) 623
EcoRII CCWGG 2 cut(s) 265, 596
EcoT38I GRGCYC 1 cut(s) 625
FaeI CATG 2 cut(s) 167, 217
FaqI GGGAC 1 cut(s) 558
FatI CATG 2 cut(s) 163, 213
Fnu4HI GCNGC 6 cut(s) 351, 354, 426, 513, 652, 655
FokI GGATG 2 cut(s) 212, 649
FriOI GRGCYC 1 cut(s) 625
Fsp4HI GCNGC 6 cut(s) 351, 354, 426, 513, 652, 655
FspBI CTAG 3 cut(s) 392, 477, 507
GluI GCNGC 6 cut(s) 351, 354, 426, 513, 652, 655
GsaI CCCAGC 1 cut(s) 99
HgaI GACGC 1 cut(s) 192
Hin1II CATG 2 cut(s) 167, 217
HinfI GANTC 5 cut(s) 32, 232, 238, 473, 601
Hpy188I TCNGA 5 cut(s) 16, 237, 301, 337, 573
Hpy188III TCNNGA 3 cut(s) 242, 459, 605
HpyCH4IV ACGT 2 cut(s) 285, 468
HpyCH4V TGCA 5 cut(s) 4, 425, 455, 661, 682
HpyF10VI GCNNNNNNNGC 5 cut(s) 223, 362, 448, 512, 521
HpyF3I CTNAG 1 cut(s) 336
HpySE526I ACGT 2 cut(s) 285, 468
Hsp92II CATG 2 cut(s) 167, 217
Kzo9I GATC 4 cut(s) 11, 16, 301, 388
LmnI GCTCC 3 cut(s) 165, 178, 620
Lsp1109I GCAGC 5 cut(s) 337, 340, 437, 499, 641
LweI GCATC 3 cut(s) 134, 190, 464
MaeI CTAG 3 cut(s) 392, 477, 507
MaeII ACGT 2 cut(s) 285, 468
MaeIII GTNAC 1 cut(s) 327
MalI GATC 4 cut(s) 13, 18, 303, 390
MbiI CCGCTC 1 cut(s) 651
MboI GATC 4 cut(s) 11, 16, 301, 388
MboII GAAGA 2 cut(s) 27, 642
MflI RGATCY 1 cut(s) 16
MhlI GDGCHC 2 cut(s) 274, 625
MluCI AATT 2 cut(s) 314, 578
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 1 cut(s) 584
MnlI CCTC 6 cut(s) 81, 130, 331, 428, 514, 652
MseI TTAA 3 cut(s) 65, 116, 189
MspCI CTTAAG 1 cut(s) 188
MspR9I CCNGG 2 cut(s) 267, 598
MvaI CCWGG 2 cut(s) 267, 598
MwoI GCNNNNNNNGC 5 cut(s) 223, 362, 448, 512, 521
NdeII GATC 4 cut(s) 11, 16, 301, 388
NlaIII CATG 2 cut(s) 167, 217
NlaIV GGNNCC 3 cut(s) 161, 180, 271
NmeAIII GCCGAG 1 cut(s) 101
NspI RCATGY 1 cut(s) 217
PfeI GAWTC 4 cut(s) 232, 238, 473, 601
PkrI GCNGC 6 cut(s) 352, 355, 427, 514, 653, 656
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
Psp124BI GAGCTC 1 cut(s) 625
Psp6I CCWGG 2 cut(s) 265, 596
PspFI CCCAGC 1 cut(s) 95
PspGI CCWGG 2 cut(s) 265, 596
PspN4I GGNNCC 3 cut(s) 161, 180, 271
PspPI GGNCC 1 cut(s) 557
PstNI CAGNNNCTG 1 cut(s) 95
PsuI RGATCY 1 cut(s) 16
RsaI GTAC 1 cut(s) 212
RsaNI GTAC 1 cut(s) 211
SacI GAGCTC 1 cut(s) 625
SaqAI TTAA 3 cut(s) 65, 116, 189
SatI GCNGC 6 cut(s) 351, 354, 426, 513, 652, 655
Sau3AI GATC 4 cut(s) 11, 16, 301, 388
Sau96I GGNCC 1 cut(s) 557
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 2 cut(s) 267, 598
SduI GDGCHC 2 cut(s) 274, 625
SfaNI GCATC 3 cut(s) 134, 190, 464
SinI GGWCC 1 cut(s) 557
SmlI CTYRAG 3 cut(s) 173, 188, 605
SmoI CTYRAG 3 cut(s) 173, 188, 605
Sse9I AATT 2 cut(s) 314, 578
SsiI CCGC 1 cut(s) 651
SspMI CTAG 3 cut(s) 392, 477, 507
SstI GAGCTC 1 cut(s) 625
StyD4I CCNGG 2 cut(s) 265, 596
TaiI ACGT 2 cut(s) 288, 471
TasI AATT 2 cut(s) 314, 578
TauI GCSGC 1 cut(s) 654
TfiI GAWTC 4 cut(s) 232, 238, 473, 601
Tru1I TTAA 3 cut(s) 65, 116, 189
Tru9I TTAA 3 cut(s) 65, 116, 189
TscAI CASTG 1 cut(s) 523
TseI GCWGC 5 cut(s) 350, 353, 425, 512, 654
TspDTI ATGAA 1 cut(s) 75
TspRI CASTG 1 cut(s) 523
Vha464I CTTAAG 1 cut(s) 188
VpaK11BI GGWCC 1 cut(s) 557
XapI RAATTY 1 cut(s) 578
XceI RCATGY 1 cut(s) 217
XcmI CCANNNNNNNNNTGG 2 cut(s) 523, 594
XspI CTAG 3 cut(s) 392, 477, 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.