Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0012596

Physical Location & Seq

Genomic Coordinates Reverse (-)

25321558 .. 25330141

Gene Structure 8584 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2694 bp

ATGAACACAATCAGCAAAAATGCTGTATATTTCATTTTGGTTCTTTTTGCAGTATTAGTCCAAGTCAGAGGCCGTTCTTTTCGACATATTACTACTGTTACTACTAGTGCTCAATCGATTTCAGACGGTGTTGATGATCATCATCATGATGTTGGATTCAACCAAAGTAATGGGACGATCAGAAGCTCCTTCCAACTTCTCAAAGGAATTGACGCCGAGTCTGAGGAGACATGCGACCAACTGTACGGGTTCTTGCCGTGCTCGAACAGTGTTTACGGGCATGTGTTCTTGATGGTGGTGTATGAGTACTTGTTGTTCCATGGAGAGTCGTACTTGGCCGCCGGAGGTGAGCAGATCTTCAAGATTCTGGGTCCTGGTGTCTTCGGTGCTAGTGCTTTCCATGTCCTCGGCGCCCTCCCAGAGTCTTTGTTGCTTCTTGCTTCTGGATTCTTTAACACCAAGGAAATTGCTGAAGAGTATGTGTACACTGGAGTTGGATTGTTGGCAGGAACATCAATTTTGCTCCTAACAATAGTTTGGGGGACTTGTGTAATTGCTGGCCGGCAACACTTCACAAGTTGTAATGATACAAATTCGTCAACAGCTTTTTCATGGGAAAGAATACCTGCATTATTGACTAGTTGTGGTATTACAATTGATTTGGAGACCAGAGTCCTCGCAAGGATCATGATTTTCTCAGTGATTCCATTTCTTATAATGCAAATCCCCAATATCTTCCACTCCTCTTCTACGGAAAACACTTTTATCTTGATTGCTCTCGGTGTTTCTGTTGTATTCCTATTGTTGTACTTCATTTATCAGGTCTTCCGGCCCTGGGTCCAAAAAAGAAGATTAGAATTTGTGAAACATGGGCATTTGGTATCAAACATTTTACAACATGTGCAAAAACATGCGCTAGGGAGAATCCTCACTGTCCAAGGAGCACCTAATTTACGTGCCATAAGAAGGCTATTTGAGGAAATGGATGAAGATGGTGACAATCATATATCCATTCCTGAAGTAAAAAAACTTCTTCAGGAAATCAAATTCACATCATTGGAAAATGATGAGGACATAGGAATAGCAGAAGTGATGAAAAAATTTGACATCGATCATGATGGAAAAATTAATAAAGATGAATTCATGAATGGCTTCACAAAATGGCTTGATGAGTACAAGTCAGTGCATAAAGAAAATACCGAAAGATCGTTGGAGGACATATACCAGGTTTTTCTACCTTGGATTCAAAATAGAAGAAAAGAACGCGAAATGAAGAAGAATTTGATGTCAGAAGTACTAAGACACGTCCAAAGCAATTCACTTGGAGCCATTCTCACAGAAAATGGCATGCCTCATATGGAAAATATTAGAAGCTTGTTTGAACAGATTGATCTTGATGGAAATAATAATATATCAGAAGCAGAACTAAGAGAACTGATCATGAACATCAAGTCTGGTAACATTCCACTCGACGTTGATGAATCAGTTAACAAATTGGTAGAAGAACTTGACACAAGTGGAGACCATTTGATTAATGAGGAGGAATTCGTTACTGGATTAACAAAATGGATGAATAAATCCCATGATACCCAAACTCTTTCGTCATTAGAGTCGGAGGACGATCTCTACCAAAGAACTTGGGACGCAACAGATAAGTTGGTCGATGAAGAAACTAGGAGCGGTGGACCTGTTGACAAGTCATTATGGGGTTGGTTCAAGGCCATTTCATATATGGTGGTCGGGTTTGTTGTACTAGCAGTTCTAGCAGAACCTCTCATAGATAATGTTCAGGACTTCTCCGGTGCTGCAGGCGTACCTTCTTTCTTCGTAGCATTTGTGCTTGTCCCGTTAGCAACCAATGCTAGACAAGCAATTTCAGCAATTAGAGCAGCAAGTCGCCAAACACCAAGAACCACTGATTTGACATTTTCGGAGATTTATGGTGGGGTTTTTATGAACAATGTGCTGGGATTTTCTGTGCTTCTGGCCATAATTTATGTTCGTGAAATGACATGGGAATTCTCTGCAGAAGTATTAGTAGTTCTAATAGTCTGCATTGTGGTGGGTCTCATTGCAAGTTTGAGATCTACCTTTCCTCTCTGGACAGCATTCGTTGCCTTCTTGCTCTACCCAGTATCATTGCTTCTAGTCTATATTATCAATGATCCCAAGTCCAAACACCCCCAACTTCGTTCCTCGGTTTACTCCCACAGTCTCACTCTCTCTCGTCGCTCCCGCTCTCTCTCTCGCCAGAAGAAGAGACGCCTCATTGTACGTACCAGACTGCTGAAGAAGAGATTGACCTCTACCTTGAGGATGGTGGAAATGTGGTGGTCCCTTTTAGCTGCGATCCCTGCAGTGGTTGCAGGACAAGCATTTCGAGTGAAGAAAAGACGTGATGATGAGCAGAAATTAAAGAGTGCTAGAGGAAGGGAGAAGAGTTCTGATGAGATTTTTGTCTGCGAGAGGGTATGCACATCTAAGAGGATGCTGAAGAAAGTTGGTGCATTCTCAAAGGACCCAATTCCTGATACTTGTGTTACTGTGTGCGGTGTTTCTGAGCTTGACGTATGCGCTGATGCCTGTGCACTGACTATTTGTGTAAACCAACATCAATTACCAAACTGGAATGACATCTGCCTCCGAAGATGCCAGAGTGAGTGTTTGAAACTCTCTTCTTTGACTTCTAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EF-hand_7 EF-hand_8 Na_Ca_ex

Protein Analysis

897

Amino Acids

101.12

Weight (kDa)

6.25

Isoelectric Point (pI)

42.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_7	PF13499	320 - 385	1.2e-11	EF-hand domain pair
EF-hand_1	PF00036	362 - 387	8.4e-06	EF hand domain
EF-hand_7	PF13499	454 - 518	4.4e-11	EF-hand domain pair
Na_Ca_ex	PF01699	574 - 718	4.2e-14	Sodium/calcium exchanger protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012596)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g18790
malus_domestica	MD03G1238300.v1.1 MD15G1340300.v1.1
prunus_persica	Prupe.4G171600_v2.0.a1 Prupe.4G171600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0031621
rosa_laevigata	RLG00000033329
rosa_multiflora	Rmu_sc0002018.1_g000001 Rmu_sc0003681.1_g000001 Rmu_sc0014819.1_g000001 Rmu_sc0031913.1_g000001
rosa_roxburghii	Rroxscaffold_1G00048660
rosa_rugosa	Rorug05G0127100 Rorug05G0127100
rosa_samantha	Rh5AG220900 Rh5BG220400 Rh5CG243800 Rh5DG225200
rosa_wichuraiana	Rw5G020130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	716
Acc36I	ACCTGC	1 cut(s)	634
AccB1I	GGYRCC	1 cut(s)	410
AccBSI	CCGCTC	2 cut(s)	1680, 2238
AccII	CGCG	1 cut(s)	1266
AciI	CCGC	4 cut(s)	339, 1680, 2236, 2550
AclWI	GGATC	3 cut(s)	692, 2159, 2344
AcoI	YGGCCR	3 cut(s)	336, 559, 1986
AcsI	RAATTY	8 cut(s)	592, 857, 1046, 1100, 1139, 1279, 1544, 2018
AcuI	CTGAAG	5 cut(s)	492, 1019, 1038, 2309, 2513
AcyI	GRCGYC	3 cut(s)	213, 411, 2263
AfiI	CCNNNNNNNGG	3 cut(s)	835, 966, 2359
AflIII	ACRYGT	2 cut(s)	898, 1303
AgsI	TTSAA	6 cut(s)	160, 361, 1247, 1382, 1717, 2668
AhdI	GACNNNNNGTC	1 cut(s)	217
AhlI	ACTAGT	2 cut(s)	104, 638
AjiI	CACGTC	2 cut(s)	1306, 2396
AjnI	CCWGG	3 cut(s)	373, 833, 1224
AluBI	AGCT	5 cut(s)	186, 605, 1374, 2345, 2563
AluI	AGCT	5 cut(s)	186, 605, 1374, 2345, 2563
Alw21I	GWGCWC	4 cut(s)	112, 263, 946, 2590
Alw26I	GTCTC	6 cut(s)	221, 659, 1515, 2072, 2219, 2254
Alw44I	GTGCAC	1 cut(s)	2586
AlwI	GGATC	3 cut(s)	692, 2159, 2344
AoxI	GGCC	6 cut(s)	70, 336, 559, 830, 1719, 1986
ApaLI	GTGCAC	1 cut(s)	2586
ApeKI	GCWGC	3 cut(s)	1805, 1889, 2345
ApoI	RAATTY	8 cut(s)	592, 857, 1046, 1100, 1139, 1279, 1544, 2018
ArsI	GACNNNNNNTTYG	2 cut(s)	2361, 2393
AseI	ATTAAT	2 cut(s)	1128, 1533
Asp700I	GAANNNNTTC	1 cut(s)	1151
AspLEI	GCGC	3 cut(s)	413, 916, 2576
AspS9I	GGNCC	6 cut(s)	371, 831, 838, 1685, 2334, 2518
AsuHPI	GGTGA	2 cut(s)	359, 1007
AvaII	GGWCC	5 cut(s)	371, 838, 1685, 2334, 2518
BaeGI	GKGCMC	1 cut(s)	2590
BaeI	ACNNNNGTAYC	2 cut(s)	2523, 2556
BalI	TGGCCA	1 cut(s)	1988
BanI	GGYRCC	1 cut(s)	410
BarI	GAAGNNNNNNTAC	4 cut(s)	1014, 1046, 2127, 2159
BbsI	GAAGAC	2 cut(s)	373, 817
Bbv12I	GWGCWC	4 cut(s)	112, 263, 946, 2590
BbvI	GCAGC	3 cut(s)	1792, 1901, 2332
BccI	CCATC	5 cut(s)	286, 986, 1112, 1391, 2311
BceAI	ACGGC	2 cut(s)	57, 241
BciT130I	CCWGG	3 cut(s)	375, 835, 1226
BclI	TGATCA	2 cut(s)	136, 1437
BcoDI	GTCTC	6 cut(s)	221, 659, 1515, 2072, 2219, 2254
BcuI	ACTAGT	2 cut(s)	104, 638
BfmI	CTRYAG	3 cut(s)	1806, 2025, 2355
BfoI	RGCGCY	1 cut(s)	414
BfuAI	ACCTGC	1 cut(s)	634
BglII	AGATCT	2 cut(s)	354, 2084
BisI	GCNGC	4 cut(s)	339, 1806, 1890, 2346
BlsI	GCNGC	4 cut(s)	340, 1807, 1891, 2347
BmcAI	AGTACT	2 cut(s)	308, 1296
Bme1390I	CCNGG	3 cut(s)	375, 835, 1226
Bme18I	GGWCC	5 cut(s)	371, 838, 1685, 2334, 2518
BmeRI	GACNNNNNGTC	1 cut(s)	217
BmgBI	CACGTC	2 cut(s)	1306, 2396
BmgT120I	GGNCC	6 cut(s)	371, 831, 838, 1685, 2334, 2518
BmiI	GGNNCC	6 cut(s)	372, 412, 839, 1327, 2336, 2520
BmrFI	CCNGG	3 cut(s)	375, 835, 1226
BmrI	ACTGGG	1 cut(s)	2126
BmsI	GCATC	3 cut(s)	2478, 2569, 2639
BmuI	ACTGGG	1 cut(s)	2126
BoxI	GACNNNNGTC	1 cut(s)	671
BpiI	GAAGAC	2 cut(s)	373, 817
BplI	GAGNNNNNCTC	2 cut(s)	1317, 1349
BpmI	CTGGAG	1 cut(s)	510
BpuEI	CTTGAG	1 cut(s)	2332
Bsa29I	ATCGAT	2 cut(s)	116, 1110
BsaAI	YACGTR	2 cut(s)	956, 2276
BsaBI	GATNNNNATC	2 cut(s)	138, 141
BsaHI	GRCGYC	3 cut(s)	213, 411, 2263
BsaI	GGTCTC	3 cut(s)	659, 1515, 2072
BsaJI	CCNNGG	8 cut(s)	319, 406, 459, 833, 834, 937, 1238, 2196
BsaWI	WCCGGW	1 cut(s)	1799
Bsc4I	CCNNNNNNNGG	3 cut(s)	835, 966, 2359
Bse118I	RCCGGY	1 cut(s)	561
Bse1I	ACTGG	4 cut(s)	493, 1558, 2132, 2630
Bse3DI	GCAATG	2 cut(s)	2070, 2138
Bse8I	GATNNNNATC	2 cut(s)	138, 141
BseBI	CCWGG	3 cut(s)	375, 835, 1226
BseCI	ATCGAT	2 cut(s)	116, 1110
BseDI	CCNNGG	8 cut(s)	319, 406, 459, 833, 834, 937, 1238, 2196
BseGI	GGATG	4 cut(s)	991, 1575, 2322, 2493
BseJI	GATNNNNATC	2 cut(s)	138, 141
BseLI	CCNNNNNNNGG	3 cut(s)	835, 966, 2359
BseMI	GCAATG	2 cut(s)	2070, 2138
BseMII	CTCAG	3 cut(s)	213, 711, 2550
BseNI	ACTGG	4 cut(s)	493, 1558, 2132, 2630
BseRI	GAGGAG	3 cut(s)	239, 733, 1553
BseSI	GKGCMC	1 cut(s)	2590
BseXI	GCAGC	3 cut(s)	1792, 1901, 2332
BseYI	CCCAGC	1 cut(s)	1966
Bsh1236I	CGCG	1 cut(s)	1266
BshFI	GGCC	6 cut(s)	72, 338, 561, 832, 1721, 1988
BshNI	GGYRCC	1 cut(s)	410
BshVI	ATCGAT	2 cut(s)	116, 1110
BsiHKAI	GWGCWC	4 cut(s)	112, 263, 946, 2590
BsiSI	CCGG	4 cut(s)	342, 562, 829, 1800
BslFI	GGGAC	5 cut(s)	187, 556, 1655, 1829, 2320
BslI	CCNNNNNNNGG	3 cut(s)	835, 966, 2359
BsmAI	GTCTC	6 cut(s)	221, 659, 1515, 2072, 2219, 2254
BsmBI	CGTCTC	1 cut(s)	2254
BsmFI	GGGAC	5 cut(s)	187, 556, 1655, 1829, 2320
BsmI	GAATGC	2 cut(s)	2108, 2507
BsnI	GGCC	6 cut(s)	72, 338, 561, 832, 1721, 1988
Bso31I	GGTCTC	3 cut(s)	659, 1515, 2072
Bsp1286I	GDGCHC	4 cut(s)	112, 263, 946, 2590
Bsp1407I	TGTACA	1 cut(s)	483
Bsp19I	CCATGG	1 cut(s)	319
BspACI	CCGC	4 cut(s)	339, 1680, 2236, 2550
BspANI	GGCC	6 cut(s)	72, 338, 561, 832, 1721, 1988
BspCNI	CTCAG	3 cut(s)	214, 710, 2551
BspDI	ATCGAT	2 cut(s)	116, 1110
BspFNI	CGCG	1 cut(s)	1266
BspHI	TCATGA	5 cut(s)	145, 687, 1114, 1143, 1440
BspLI	GGNNCC	6 cut(s)	372, 412, 839, 1327, 2336, 2520
BspMAI	CTGCAG	3 cut(s)	1810, 2029, 2359
BspMI	ACCTGC	1 cut(s)	634
BspPI	GGATC	3 cut(s)	692, 2159, 2344
BspT107I	GGYRCC	1 cut(s)	410
BspTNI	GGTCTC	3 cut(s)	659, 1515, 2072
BsrBI	CCGCTC	2 cut(s)	1680, 2238
BsrDI	GCAATG	2 cut(s)	2070, 2138
BsrFI	RCCGGY	1 cut(s)	561
BsrGI	TGTACA	1 cut(s)	483
BsrI	ACTGG	4 cut(s)	493, 1558, 2132, 2630
BssAI	RCCGGY	1 cut(s)	561
BssECI	CCNNGG	8 cut(s)	319, 406, 459, 833, 834, 937, 1238, 2196
BssNI	GRCGYC	3 cut(s)	213, 411, 2263
BssT1I	CCWWGG	4 cut(s)	319, 459, 937, 1238
Bst2UI	CCWGG	3 cut(s)	375, 835, 1226
Bst4CI	ACNGT	7 cut(s)	97, 128, 243, 269, 934, 2213, 2545
Bst6I	CTCTTC	6 cut(s)	468, 751, 2252, 2288, 2432, 2680
BstACI	GRCGYC	3 cut(s)	213, 411, 2263
BstAPI	GCANNNNNTGC	3 cut(s)	1859, 2114, 2363
BstAUI	TGTACA	1 cut(s)	483
BstBAI	YACGTR	2 cut(s)	956, 2276
BstC8I	GCNNGC	4 cut(s)	559, 563, 1349, 1810
BstDEI	CTNAG	6 cut(s)	222, 697, 1298, 1427, 2481, 2559
BstDSI	CCRYGG	1 cut(s)	319
BstF5I	GGATG	4 cut(s)	991, 1575, 2322, 2493
BstFNI	CGCG	1 cut(s)	1266
BstH2I	RGCGCY	1 cut(s)	414
BstHHI	GCGC	3 cut(s)	413, 916, 2576
BstMAI	GTCTC	6 cut(s)	221, 659, 1515, 2072, 2219, 2254
BstMWI	GCNNNNNNNGC	9 cut(s)	1763, 1859, 1868, 1877, 1886, 2114, 2354, 2363, 2372
BstNI	CCWGG	3 cut(s)	375, 835, 1226
BstNSI	RCATGY	5 cut(s)	234, 284, 902, 914, 1351
BstPAI	GACNNNNGTC	1 cut(s)	671
BstSCI	CCNGG	3 cut(s)	373, 833, 1224
BstSFI	CTRYAG	3 cut(s)	1806, 2025, 2355
BstSLI	GKGCMC	1 cut(s)	2590
BstSNI	TACGTA	1 cut(s)	2276
BstUI	CGCG	1 cut(s)	1266
BstV1I	GCAGC	3 cut(s)	1792, 1901, 2332
BstV2I	GAAGAC	2 cut(s)	373, 817
BstX2I	RGATCY	2 cut(s)	354, 2084
BstXI	CCANNNNNNTGG	1 cut(s)	170
BstYI	RGATCY	2 cut(s)	354, 2084
Bsu15I	ATCGAT	2 cut(s)	116, 1110
BsuRI	GGCC	6 cut(s)	72, 338, 561, 832, 1721, 1988
BsuTUI	ATCGAT	2 cut(s)	116, 1110
BtgI	CCRYGG	1 cut(s)	319
BtrI	CACGTC	2 cut(s)	1306, 2396
BtsCI	GGATG	4 cut(s)	991, 1575, 2322, 2493
BtsI	GCAGTG	1 cut(s)	2364
BtsIMutI	CAGTG	8 cut(s)	274, 486, 705, 930, 1188, 1914, 2364, 2588
BveI	ACCTGC	1 cut(s)	634
Cac8I	GCNNGC	4 cut(s)	559, 563, 1349, 1810
CciI	TCATGA	5 cut(s)	145, 687, 1114, 1143, 1440
CfoI	GCGC	3 cut(s)	413, 916, 2576
Cfr10I	RCCGGY	1 cut(s)	561
Cfr13I	GGNCC	6 cut(s)	371, 831, 838, 1685, 2334, 2518
ClaI	ATCGAT	2 cut(s)	116, 1110
CseI	GACGC	3 cut(s)	221, 1652, 2271
CsiI	ACCWGGT	1 cut(s)	1224
DdeI	CTNAG	6 cut(s)	222, 697, 1298, 1427, 2481, 2559
DinI	GGCGCC	1 cut(s)	412
DriI	GACNNNNNGTC	1 cut(s)	217
EaeI	YGGCCR	3 cut(s)	336, 559, 1986
Eam1104I	CTCTTC	6 cut(s)	468, 751, 2252, 2288, 2432, 2680
Eam1105I	GACNNNNNGTC	1 cut(s)	217
EarI	CTCTTC	6 cut(s)	468, 751, 2252, 2288, 2432, 2680
Eco105I	TACGTA	1 cut(s)	2276
Eco130I	CCWWGG	4 cut(s)	319, 459, 937, 1238
Eco31I	GGTCTC	3 cut(s)	659, 1515, 2072
Eco47I	GGWCC	5 cut(s)	371, 838, 1685, 2334, 2518
Eco57I	CTGAAG	5 cut(s)	492, 1019, 1038, 2309, 2513
EcoO109I	RGGNCCY	2 cut(s)	371, 2518
EcoRI	GAATTC	3 cut(s)	1139, 1544, 2018
EcoRII	CCWGG	3 cut(s)	373, 833, 1224
EcoT14I	CCWWGG	4 cut(s)	319, 459, 937, 1238
EgeI	GGCGCC	1 cut(s)	412
EheI	GGCGCC	1 cut(s)	412
ErhI	CCWWGG	4 cut(s)	319, 459, 937, 1238
Esp3I	CGTCTC	1 cut(s)	2254
FaqI	GGGAC	5 cut(s)	187, 556, 1655, 1829, 2320
FauI	CCCGC	1 cut(s)	2243
FauNDI	CATATG	1 cut(s)	1356
FbaI	TGATCA	2 cut(s)	136, 1437
Fnu4HI	GCNGC	4 cut(s)	339, 1806, 1890, 2346
FokI	GGATG	4 cut(s)	998, 1582, 2329, 2500
Fsp4HI	GCNGC	4 cut(s)	339, 1806, 1890, 2346
GlaI	GCGC	3 cut(s)	412, 915, 2575
GluI	GCNGC	4 cut(s)	339, 1806, 1890, 2346
GsaI	CCCAGC	1 cut(s)	1970
GsuI	CTGGAG	1 cut(s)	510
HaeII	RGCGCY	1 cut(s)	414
HaeIII	GGCC	6 cut(s)	72, 338, 561, 832, 1721, 1988
HapII	CCGG	4 cut(s)	342, 562, 829, 1800
HgaI	GACGC	3 cut(s)	221, 1652, 2271
HhaI	GCGC	3 cut(s)	413, 916, 2576
Hin1I	GRCGYC	3 cut(s)	213, 411, 2263
Hin6I	GCGC	3 cut(s)	411, 914, 2574
HinP1I	GCGC	3 cut(s)	411, 914, 2574
HincII	GTYRAC	3 cut(s)	600, 1489, 1693
HindII	GTYRAC	3 cut(s)	600, 1489, 1693
HindIII	AAGCTT	1 cut(s)	1372
HpaI	GTTAAC	1 cut(s)	1489
HpaII	CCGG	4 cut(s)	342, 562, 829, 1800
HphI	GGTGA	2 cut(s)	359, 1007
Hpy99I	CGWCG	2 cut(s)	1475, 2232
HpyAV	CCTTC	5 cut(s)	199, 960, 1827, 2128, 2424
HpyCH4III	ACNGT	7 cut(s)	97, 128, 243, 269, 934, 2213, 2545
HpyCH4IV	ACGT	6 cut(s)	955, 1305, 1473, 2275, 2395, 2568
HpyF10VI	GCNNNNNNNGC	9 cut(s)	1763, 1859, 1868, 1877, 1886, 2114, 2354, 2363, 2372
HpyF3I	CTNAG	6 cut(s)	222, 697, 1298, 1427, 2481, 2559
HpySE526I	ACGT	6 cut(s)	955, 1305, 1473, 2275, 2395, 2568
Hsp92I	GRCGYC	3 cut(s)	213, 411, 2263
HspAI	GCGC	3 cut(s)	411, 914, 2574
KasI	GGCGCC	1 cut(s)	410
KroI	GCCGGC	1 cut(s)	561
KroNI	GCCGGC	1 cut(s)	563
Ksp22I	TGATCA	2 cut(s)	136, 1437
KspAI	GTTAAC	1 cut(s)	1489
LmnI	GCTCC	6 cut(s)	191, 528, 941, 1325, 1677, 2237
Lsp1109I	GCAGC	3 cut(s)	1792, 1901, 2332
LweI	GCATC	3 cut(s)	2478, 2569, 2639
MabI	ACCWGGT	1 cut(s)	1224
MaeII	ACGT	6 cut(s)	955, 1305, 1473, 2275, 2395, 2568
MaeIII	GTNAC	5 cut(s)	97, 995, 1457, 1549, 2539
MbiI	CCGCTC	2 cut(s)	1680, 2238
MfeI	CAATTG	1 cut(s)	654
MflI	RGATCY	2 cut(s)	354, 2084
MhlI	GDGCHC	4 cut(s)	112, 263, 946, 2590
MlsI	TGGCCA	1 cut(s)	1988
MluNI	TGGCCA	1 cut(s)	1988
Mly113I	GGCGCC	1 cut(s)	411
MlyI	GAGTC	5 cut(s)	227, 335, 431, 681, 1619
MmeI	TCCRAC	5 cut(s)	133, 217, 475, 1191, 1593
Mox20I	TGGCCA	1 cut(s)	1988
MroNI	GCCGGC	1 cut(s)	561
MroXI	GAANNNNTTC	1 cut(s)	1151
MscI	TGGCCA	1 cut(s)	1988
MseI	TTAA	6 cut(s)	453, 1128, 1488, 1533, 1559, 2414
MslI	CAYNNNNRTG	3 cut(s)	144, 147, 2060
Msp20I	TGGCCA	1 cut(s)	1988
MspI	CCGG	4 cut(s)	342, 562, 829, 1800
MspR9I	CCNGG	3 cut(s)	375, 835, 1226
MunI	CAATTG	1 cut(s)	654
Mva1269I	GAATGC	2 cut(s)	2108, 2507
MvaI	CCWGG	3 cut(s)	375, 835, 1226
MvnI	CGCG	1 cut(s)	1266
MwoI	GCNNNNNNNGC	9 cut(s)	1763, 1859, 1868, 1877, 1886, 2114, 2354, 2363, 2372
NaeI	GCCGGC	1 cut(s)	563
NarI	GGCGCC	1 cut(s)	411
NcoI	CCATGG	1 cut(s)	319
NdeI	CATATG	1 cut(s)	1356
NgoMIV	GCCGGC	1 cut(s)	561
NlaIV	GGNNCC	6 cut(s)	372, 412, 839, 1327, 2336, 2520
NmeAIII	GCCGAG	2 cut(s)	241, 387
NmuCI	GTSAC	1 cut(s)	995
NspI	RCATGY	5 cut(s)	234, 284, 902, 914, 1351
PaeI	GCATGC	1 cut(s)	1351
PagI	TCATGA	5 cut(s)	145, 687, 1114, 1143, 1440
PasI	CCCWGGG	1 cut(s)	834
PciI	ACATGT	1 cut(s)	898
PctI	GAATGC	2 cut(s)	2108, 2507
PdiI	GCCGGC	1 cut(s)	563
PdmI	GAANNNNTTC	1 cut(s)	1151
PfeI	GAWTC	7 cut(s)	156, 364, 447, 703, 924, 1243, 1481
PkrI	GCNGC	4 cut(s)	340, 1807, 1891, 2347
PleI	GAGTC	5 cut(s)	226, 334, 430, 680, 1618
PluTI	GGCGCC	1 cut(s)	414
PpsI	GAGTC	5 cut(s)	226, 334, 430, 680, 1618
Ppu21I	YACGTR	2 cut(s)	956, 2276
PpuMI	RGGWCCY	2 cut(s)	371, 2518
PscI	ACATGT	1 cut(s)	898
PshAI	GACNNNNGTC	1 cut(s)	671
PshBI	ATTAAT	2 cut(s)	1128, 1533
PsiI	TTATAA	1 cut(s)	716
Psp5II	RGGWCCY	2 cut(s)	371, 2518
Psp6I	CCWGG	3 cut(s)	373, 833, 1224
PspFI	CCCAGC	1 cut(s)	1966
PspGI	CCWGG	3 cut(s)	373, 833, 1224
PspN4I	GGNNCC	6 cut(s)	372, 412, 839, 1327, 2336, 2520
PspPI	GGNCC	6 cut(s)	371, 831, 838, 1685, 2334, 2518
PspPPI	RGGWCCY	2 cut(s)	371, 2518
PsrI	GAACNNNNNNTAC	8 cut(s)	257, 289, 299, 331, 1743, 1775, 2025, 2057
PstI	CTGCAG	3 cut(s)	1810, 2029, 2359
PsuI	RGATCY	2 cut(s)	354, 2084
RseI	CAYNNNNRTG	3 cut(s)	144, 147, 2060
SaqAI	TTAA	6 cut(s)	453, 1128, 1488, 1533, 1559, 2414
SatI	GCNGC	4 cut(s)	339, 1806, 1890, 2346
Sau96I	GGNCC	6 cut(s)	371, 831, 838, 1685, 2334, 2518
ScaI	AGTACT	2 cut(s)	308, 1296
SchI	GAGTC	5 cut(s)	227, 335, 431, 681, 1619
ScrFI	CCNGG	3 cut(s)	375, 835, 1226
SduI	GDGCHC	4 cut(s)	112, 263, 946, 2590
SexAI	ACCWGGT	1 cut(s)	1224
SfaNI	GCATC	3 cut(s)	2478, 2569, 2639
SfcI	CTRYAG	3 cut(s)	1806, 2025, 2355
SfoI	GGCGCC	1 cut(s)	412
SinI	GGWCC	5 cut(s)	371, 838, 1685, 2334, 2518
SmiMI	CAYNNNNRTG	3 cut(s)	144, 147, 2060
SmlI	CTYRAG	1 cut(s)	2311
SmoI	CTYRAG	1 cut(s)	2311
SnaBI	TACGTA	1 cut(s)	2276
SpeI	ACTAGT	2 cut(s)	104, 638
SphI	GCATGC	1 cut(s)	1351
SsiI	CCGC	4 cut(s)	339, 1680, 2236, 2550
SspDI	GGCGCC	1 cut(s)	410
SspI	AATATT	1 cut(s)	1366
StyD4I	CCNGG	3 cut(s)	373, 833, 1224
StyI	CCWWGG	4 cut(s)	319, 459, 937, 1238
TaaI	ACNGT	7 cut(s)	97, 128, 243, 269, 934, 2213, 2545
TaiI	ACGT	6 cut(s)	958, 1308, 1476, 2278, 2398, 2571
TaqI	TCGA	7 cut(s)	82, 116, 263, 1110, 1470, 1662, 2380
TatI	WGTACW	6 cut(s)	306, 483, 807, 1173, 1294, 1750
TauI	GCSGC	1 cut(s)	341
TfiI	GAWTC	7 cut(s)	156, 364, 447, 703, 924, 1243, 1481
Tru1I	TTAA	6 cut(s)	453, 1128, 1488, 1533, 1559, 2414
Tru9I	TTAA	6 cut(s)	453, 1128, 1488, 1533, 1559, 2414
TscAI	CASTG	8 cut(s)	274, 493, 705, 937, 1188, 1921, 2364, 2595
TseFI	GTSAC	1 cut(s)	995
TseI	GCWGC	3 cut(s)	1805, 1889, 2345
Tsp45I	GTSAC	1 cut(s)	995
TspGWI	ACGGA	1 cut(s)	767
TspRI	CASTG	8 cut(s)	274, 493, 705, 937, 1188, 1921, 2364, 2595
VneI	GTGCAC	1 cut(s)	2586
VpaK11BI	GGWCC	5 cut(s)	371, 838, 1685, 2334, 2518
VspI	ATTAAT	2 cut(s)	1128, 1533
XapI	RAATTY	8 cut(s)	592, 857, 1046, 1100, 1139, 1279, 1544, 2018
XceI	RCATGY	5 cut(s)	234, 284, 902, 914, 1351
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1729
XmnI	GAANNNNTTC	1 cut(s)	1151
ZrmI	AGTACT	2 cut(s)	308, 1296

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.