Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0011204

Physical Location & Seq

Genomic Coordinates Reverse (-)

33821745 .. 33822712

Gene Structure 968 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 921 bp

ATGTCTATCTTCACTCAAAATAAGCTCCTCCCAAGCCCCAAACACCATGTTCACACAATATGCCACTCTCCAATCCTCCGAACAACCGTACATTGCTCTGTCTCCACCACCGCAAAGCCGAAACCAGTCCCCGAAAAGCTCATCATCGAACCTCCCCGGCTGCAGCAGCTGGAACCCTCACCGGACAACATCCGGCCGATGCTCAATGACCCGAGTCTACTATCAACGTGGTCTCACCGTGCATGGGTAGCGAGCGGGTGCACTACTGTGCTCATTTCTCTAGCTAAATCCGTAGCGGGTGCAGCCCATTCACACATATGGGCCGAGCCCATTTTAGCAGGCTTGGTCGGGTACGTGTTGGCGGATCTCGGATCTGGAGTTTACCATTGGGGCATTGACAACTATGGTGGTGCCTCAACTCCAATTTTTGGTGCCCAAATAGAGGCATTCCAAGGTCACCATAAGTGGCCATGGACAATCACTAAGCGCCAATTTGCTAACAATTTACATGCCTTGGCTCGTGTCATAACTTTCACAGTGCTTCCCTTAGACCTTGCCTTTGATGATCCTATTTTTCATGGCTTTGTTGCTGTTTGCTCTGGTTGCATTATGTTTAGCCAGCAATTTCATGCTTGGGCTCATGGCACAAAGAGCCGGCTACCTCCATTGGTGCTGGCATTGCAAGATTCCGGCTTACTGATATCTCGGTCGCAACATTCGGCGCACCACCGGCAACCTTATAACAACAACTACTGCATAGTGAGTGGAGTTTGGAATGAGTTTTTGGATAAGCAGAAGGTATTTGAAGCGTTGGAGATGATCTTGTTTTTTAAGCAAGGGTTAAGACCTAGGTCTTGGAGTGAACCTTGCTCTGAGTGGATTGAAGAGGCTGAGACTATTTCTCAACTTGCAAACAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006082 GO:0006464 GO:0006508 GO:0006511 GO:0006629 GO:0006631 GO:0006633 GO:0006636 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009507 GO:0009526 GO:0009536 GO:0009628 GO:0009941 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0016020 GO:0016053 GO:0016491 GO:0016567 GO:0016705 GO:0016740 GO:0019538 GO:0019637 GO:0019752 GO:0019787 GO:0019899 GO:0019941 GO:0030163 GO:0030433 GO:0031090 GO:0031625 GO:0031967 GO:0031969 GO:0031975 GO:0032446 GO:0032787 GO:0033554 GO:0033559 GO:0034976 GO:0036211 GO:0036503 GO:0042170 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044281 GO:0044283 GO:0044389 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046471 GO:0046486 GO:0050896 GO:0051603 GO:0051716 GO:0052637 GO:0055114 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0072330 GO:0080167 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1901698

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TMEM189_B_dmain

Protein Analysis

306

Amino Acids

34.13

Weight (kDa)

7.75

Isoelectric Point (pI)

47.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipid_desat	PF10520	118 - 286	4.5e-70	Lipid desaturase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011204)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27030
fragaria_vesca	FvH4_3g22400
malus_domestica	MD03G1205300.v1.1 MD11G1221200.v1.1
prunus_persica	Prupe.4G210500_v2.0.a1 Prupe.4G210600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0039071
rosa_laevigata	RLG00000033888 RLG00000033890 RLG00000033891
rosa_multiflora	Rmu_co8409277.1_g000001 Rmu_sc0000533.1_g000033 Rmu_sc0007753.1_g000004
rosa_roxburghii	Rroxscaffold_1G00042040
rosa_rugosa	Rorug05G0176300
rosa_samantha	Rh5AG262700 Rh5BG267500 Rh5CG300200 Rh5DG273900
rosa_wichuraiana	Rw5G024560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	741
AccB1I	GGYRCC	2 cut(s)	410, 431
AccB7I	CCANNNNNTGG	1 cut(s)	428
AccBSI	CCGCTC	1 cut(s)	255
AccI	GTMKAC	1 cut(s)	217
AciI	CCGC	4 cut(s)	111, 255, 296, 362
AclWI	GGATC	3 cut(s)	372, 379, 560
AcoI	YGGCCR	2 cut(s)	194, 467
AfaI	GTAC	2 cut(s)	90, 353
AfiI	CCNNNNNNNGG	3 cut(s)	244, 428, 442
AflIII	ACRYGT	1 cut(s)	354
AgsI	TTSAA	2 cut(s)	806, 884
AjuI	GAANNNNNNNTTGG	2 cut(s)	767, 799
AleI	CACNNNNGTG	1 cut(s)	266
AluBI	AGCT	4 cut(s)	25, 139, 169, 284
AluI	AGCT	4 cut(s)	25, 139, 169, 284
Alw21I	GWGCWC	2 cut(s)	263, 273
Alw26I	GTCTC	3 cut(s)	106, 237, 887
Alw44I	GTGCAC	1 cut(s)	259
AlwI	GGATC	3 cut(s)	372, 379, 560
AlwNI	CAGNNNCTG	1 cut(s)	169
Ama87I	CYCGRG	1 cut(s)	211
AoxI	GGCC	3 cut(s)	194, 321, 467
ApaLI	GTGCAC	1 cut(s)	259
ApeKI	GCWGC	4 cut(s)	160, 163, 166, 302
ArsI	GACNNNNNNTTYG	2 cut(s)	542, 574
AspA2I	CCTAGG	1 cut(s)	848
AspLEI	GCGC	2 cut(s)	489, 724
AspS9I	GGNCC	1 cut(s)	321
AsuC2I	CCSGG	1 cut(s)	157
AsuHPI	GGTGA	3 cut(s)	171, 227, 449
AvaI	CYCGRG	1 cut(s)	211
AvrII	CCTAGG	1 cut(s)	848
BaeGI	GKGCMC	2 cut(s)	263, 436
BalI	TGGCCA	1 cut(s)	469
BanI	GGYRCC	2 cut(s)	410, 431
BanII	GRGCYC	2 cut(s)	330, 640
BauI	CACGAG	1 cut(s)	519
Bbv12I	GWGCWC	2 cut(s)	263, 273
BbvI	GCAGC	4 cut(s)	147, 175, 178, 314
BcnI	CCSGG	1 cut(s)	157
BcoDI	GTCTC	3 cut(s)	106, 237, 887
BfaI	CTAG	2 cut(s)	281, 849
BfmI	CTRYAG	1 cut(s)	161
BfoI	RGCGCY	1 cut(s)	490
BisI	GCNGC	4 cut(s)	161, 164, 167, 303
BlnI	CCTAGG	1 cut(s)	848
BlsI	GCNGC	4 cut(s)	162, 165, 168, 304
Bme1390I	CCNGG	1 cut(s)	157
BmeT110I	CYCGRG	1 cut(s)	211
BmgT120I	GGNCC	1 cut(s)	321
BmiI	GGNNCC	3 cut(s)	174, 412, 433
BmrFI	CCNGG	1 cut(s)	157
BmsI	GCATC	1 cut(s)	189
BoxI	GACNNNNGTC	2 cut(s)	213, 850
BpmI	CTGGAG	1 cut(s)	396
BpuMI	CCSGG	1 cut(s)	157
BsaAI	YACGTR	1 cut(s)	355
BsaI	GGTCTC	1 cut(s)	237
BsaJI	CCNNGG	5 cut(s)	155, 451, 470, 513, 848
BsaWI	WCCGGW	1 cut(s)	181
Bsc4I	CCNNNNNNNGG	3 cut(s)	244, 428, 442
Bse118I	RCCGGY	2 cut(s)	654, 729
Bse1I	ACTGG	1 cut(s)	125
Bse3DI	GCAATG	2 cut(s)	91, 677
BseDI	CCNNGG	5 cut(s)	155, 451, 470, 513, 848
BseGI	GGATG	1 cut(s)	189
BseLI	CCNNNNNNNGG	3 cut(s)	244, 428, 442
BseMI	GCAATG	2 cut(s)	91, 677
BseMII	CTCAG	2 cut(s)	864, 882
BseNI	ACTGG	1 cut(s)	125
BseRI	GAGGAG	1 cut(s)	17
BseSI	GKGCMC	2 cut(s)	263, 436
BseX3I	CGGCCG	1 cut(s)	194
BseXI	GCAGC	4 cut(s)	147, 175, 178, 314
BsgI	GTGCAG	1 cut(s)	321
Bsh1285I	CGRYCG	2 cut(s)	197, 710
BshFI	GGCC	3 cut(s)	196, 323, 469
BshNI	GGYRCC	2 cut(s)	410, 431
BsiEI	CGRYCG	2 cut(s)	197, 710
BsiHKAI	GWGCWC	2 cut(s)	263, 273
BsiHKCI	CYCGRG	1 cut(s)	211
BsiSI	CCGG	6 cut(s)	157, 182, 193, 655, 690, 730
BslFI	GGGAC	1 cut(s)	113
BslI	CCNNNNNNNGG	3 cut(s)	244, 428, 442
BsmAI	GTCTC	3 cut(s)	106, 237, 887
BsmFI	GGGAC	1 cut(s)	113
BsmI	GAATGC	1 cut(s)	446
BsnI	GGCC	3 cut(s)	196, 323, 469
Bso31I	GGTCTC	1 cut(s)	237
BsoBI	CYCGRG	1 cut(s)	211
Bsp1286I	GDGCHC	5 cut(s)	263, 273, 330, 436, 640
Bsp143I	GATC	4 cut(s)	364, 371, 565, 819
Bsp19I	CCATGG	1 cut(s)	470
BspACI	CCGC	4 cut(s)	111, 255, 296, 362
BspANI	GGCC	3 cut(s)	196, 323, 469
BspCNI	CTCAG	2 cut(s)	865, 883
BspLI	GGNNCC	3 cut(s)	174, 412, 433
BspMAI	CTGCAG	1 cut(s)	165
BspPI	GGATC	3 cut(s)	372, 379, 560
BspT107I	GGYRCC	2 cut(s)	410, 431
BspTNI	GGTCTC	1 cut(s)	237
BsrBI	CCGCTC	1 cut(s)	255
BsrDI	GCAATG	2 cut(s)	91, 677
BsrFI	RCCGGY	2 cut(s)	654, 729
BsrI	ACTGG	1 cut(s)	125
BssAI	RCCGGY	2 cut(s)	654, 729
BssECI	CCNNGG	5 cut(s)	155, 451, 470, 513, 848
BssMI	GATC	4 cut(s)	364, 371, 565, 819
BssSI	CACGAG	1 cut(s)	519
BssT1I	CCWWGG	4 cut(s)	451, 470, 513, 848
Bst2BI	CACGAG	1 cut(s)	519
Bst4CI	ACNGT	4 cut(s)	88, 239, 268, 538
Bst6I	CTCTTC	1 cut(s)	879
BstBAI	YACGTR	1 cut(s)	355
BstC8I	GCNNGC	5 cut(s)	253, 340, 620, 656, 675
BstDEI	CTNAG	4 cut(s)	483, 547, 873, 891
BstDSI	CCRYGG	1 cut(s)	470
BstEII	GGTNACC	1 cut(s)	455
BstF5I	GGATG	1 cut(s)	189
BstH2I	RGCGCY	1 cut(s)	490
BstHHI	GCGC	2 cut(s)	489, 724
BstKTI	GATC	4 cut(s)	367, 374, 568, 822
BstMAI	GTCTC	3 cut(s)	106, 237, 887
BstMBI	GATC	4 cut(s)	364, 371, 565, 819
BstMCI	CGRYCG	2 cut(s)	197, 710
BstMWI	GCNNNNNNNGC	7 cut(s)	166, 248, 302, 603, 651, 679, 730
BstNSI	RCATGY	1 cut(s)	512
BstPAI	GACNNNNGTC	2 cut(s)	213, 850
BstPI	GGTNACC	1 cut(s)	455
BstSCI	CCNGG	1 cut(s)	155
BstSFI	CTRYAG	1 cut(s)	161
BstSLI	GKGCMC	2 cut(s)	263, 436
BstV1I	GCAGC	4 cut(s)	147, 175, 178, 314
BstX2I	RGATCY	2 cut(s)	364, 371
BstYI	RGATCY	2 cut(s)	364, 371
BstZI	CGGCCG	1 cut(s)	194
BsuRI	GGCC	3 cut(s)	196, 323, 469
BtgI	CCRYGG	1 cut(s)	470
BtsCI	GGATG	1 cut(s)	189
BtsIMutI	CAGTG	1 cut(s)	543
Cac8I	GCNNGC	5 cut(s)	253, 340, 620, 656, 675
CaiI	CAGNNNCTG	1 cut(s)	169
CfoI	GCGC	2 cut(s)	489, 724
Cfr10I	RCCGGY	2 cut(s)	654, 729
Cfr13I	GGNCC	1 cut(s)	321
Csp6I	GTAC	2 cut(s)	89, 352
CspCI	CAANNNNNGTGG	2 cut(s)	388, 423
CviAII	CATG	7 cut(s)	47, 243, 471, 509, 578, 629, 641
CviQI	GTAC	2 cut(s)	89, 352
DdeI	CTNAG	4 cut(s)	483, 547, 873, 891
DpnI	GATC	4 cut(s)	366, 373, 567, 821
DpnII	GATC	4 cut(s)	364, 371, 565, 819
EaeI	YGGCCR	2 cut(s)	194, 467
EagI	CGGCCG	1 cut(s)	194
Eam1104I	CTCTTC	1 cut(s)	879
EarI	CTCTTC	1 cut(s)	879
EciI	GGCGGA	1 cut(s)	377
EclXI	CGGCCG	1 cut(s)	194
Eco130I	CCWWGG	4 cut(s)	451, 470, 513, 848
Eco24I	GRGCYC	2 cut(s)	330, 640
Eco31I	GGTCTC	1 cut(s)	237
Eco32I	GATATC	1 cut(s)	702
Eco52I	CGGCCG	1 cut(s)	194
Eco88I	CYCGRG	1 cut(s)	211
Eco91I	GGTNACC	1 cut(s)	455
EcoO65I	GGTNACC	1 cut(s)	455
EcoRV	GATATC	1 cut(s)	702
EcoT14I	CCWWGG	4 cut(s)	451, 470, 513, 848
EcoT38I	GRGCYC	2 cut(s)	330, 640
ErhI	CCWWGG	4 cut(s)	451, 470, 513, 848
FaeI	CATG	7 cut(s)	50, 246, 474, 512, 581, 632, 644
FaqI	GGGAC	1 cut(s)	113
FatI	CATG	7 cut(s)	46, 242, 470, 508, 577, 628, 640
FauI	CCCGC	2 cut(s)	248, 289
FauNDI	CATATG	1 cut(s)	317
FblI	GTMKAC	1 cut(s)	217
Fnu4HI	GCNGC	4 cut(s)	161, 164, 167, 303
FokI	GGATG	1 cut(s)	176
FriOI	GRGCYC	2 cut(s)	330, 640
Fsp4HI	GCNGC	4 cut(s)	161, 164, 167, 303
FspBI	CTAG	2 cut(s)	281, 849
GlaI	GCGC	2 cut(s)	488, 723
GluI	GCNGC	4 cut(s)	161, 164, 167, 303
GsuI	CTGGAG	1 cut(s)	396
HaeII	RGCGCY	1 cut(s)	490
HaeIII	GGCC	3 cut(s)	196, 323, 469
HapII	CCGG	6 cut(s)	157, 182, 193, 655, 690, 730
HhaI	GCGC	2 cut(s)	489, 724
Hin1II	CATG	7 cut(s)	50, 246, 474, 512, 581, 632, 644
Hin6I	GCGC	2 cut(s)	487, 722
HinP1I	GCGC	2 cut(s)	487, 722
HinfI	GANTC	2 cut(s)	214, 686
HpaII	CCGG	6 cut(s)	157, 182, 193, 655, 690, 730
HphI	GGTGA	3 cut(s)	171, 227, 449
Hpy166II	GTNNAC	5 cut(s)	52, 218, 261, 382, 863
Hpy188I	TCNGA	3 cut(s)	80, 371, 874
Hpy188III	TCNNGA	1 cut(s)	375
Hpy8I	GTNNAC	5 cut(s)	52, 218, 261, 382, 863
HpyAV	CCTTC	1 cut(s)	790
HpyCH4III	ACNGT	4 cut(s)	88, 239, 268, 538
HpyCH4IV	ACGT	2 cut(s)	227, 354
HpyCH4V	TGCA	8 cut(s)	163, 242, 261, 302, 606, 682, 756, 911
HpyF10VI	GCNNNNNNNGC	7 cut(s)	166, 248, 302, 603, 651, 679, 730
HpyF3I	CTNAG	4 cut(s)	483, 547, 873, 891
HpySE526I	ACGT	2 cut(s)	227, 354
Hsp92II	CATG	7 cut(s)	50, 246, 474, 512, 581, 632, 644
HspAI	GCGC	2 cut(s)	487, 722
KroI	GCCGGC	1 cut(s)	654
KroNI	GCCGGC	1 cut(s)	656
Kzo9I	GATC	4 cut(s)	364, 371, 565, 819
LmnI	GCTCC	1 cut(s)	30
Lsp1109I	GCAGC	4 cut(s)	147, 175, 178, 314
LweI	GCATC	1 cut(s)	189
MaeI	CTAG	2 cut(s)	281, 849
MaeII	ACGT	2 cut(s)	227, 354
MaeIII	GTNAC	1 cut(s)	455
MalI	GATC	4 cut(s)	366, 373, 567, 821
MbiI	CCGCTC	1 cut(s)	255
MboI	GATC	4 cut(s)	364, 371, 565, 819
MboII	GAAGA	1 cut(s)	896
MfeI	CAATTG	1 cut(s)	916
MflI	RGATCY	2 cut(s)	364, 371
MhlI	GDGCHC	5 cut(s)	263, 273, 330, 436, 640
MlsI	TGGCCA	1 cut(s)	469
MluCI	AATT	5 cut(s)	423, 491, 502, 623, 916
MluNI	TGGCCA	1 cut(s)	469
MlyI	GAGTC	1 cut(s)	223
MmeI	TCCRAC	1 cut(s)	792
MnlI	CCTC	8 cut(s)	38, 86, 162, 187, 424, 436, 672, 880
Mox20I	TGGCCA	1 cut(s)	469
MroNI	GCCGGC	1 cut(s)	654
MscI	TGGCCA	1 cut(s)	469
MseI	TTAA	2 cut(s)	831, 842
MslI	CAYNNNNRTG	2 cut(s)	266, 316
Msp20I	TGGCCA	1 cut(s)	469
MspA1I	CMGCKG	1 cut(s)	169
MspI	CCGG	6 cut(s)	157, 182, 193, 655, 690, 730
MspR9I	CCNGG	1 cut(s)	157
MunI	CAATTG	1 cut(s)	916
Mva1269I	GAATGC	1 cut(s)	446
MwoI	GCNNNNNNNGC	7 cut(s)	166, 248, 302, 603, 651, 679, 730
NaeI	GCCGGC	1 cut(s)	656
NciI	CCSGG	1 cut(s)	157
NcoI	CCATGG	1 cut(s)	470
NdeI	CATATG	1 cut(s)	317
NdeII	GATC	4 cut(s)	364, 371, 565, 819
NgoMIV	GCCGGC	1 cut(s)	654
NlaIII	CATG	7 cut(s)	50, 246, 474, 512, 581, 632, 644
NlaIV	GGNNCC	3 cut(s)	174, 412, 433
NmeAIII	GCCGAG	1 cut(s)	349
NmuCI	GTSAC	1 cut(s)	455
NspI	RCATGY	1 cut(s)	512
OliI	CACNNNNGTG	1 cut(s)	266
PctI	GAATGC	1 cut(s)	446
PdiI	GCCGGC	1 cut(s)	656
PfeI	GAWTC	1 cut(s)	686
PflMI	CCANNNNNTGG	1 cut(s)	428
PkrI	GCNGC	4 cut(s)	162, 165, 168, 304
PleI	GAGTC	1 cut(s)	222
PpsI	GAGTC	1 cut(s)	222
Ppu21I	YACGTR	1 cut(s)	355
PshAI	GACNNNNGTC	2 cut(s)	213, 850
PsiI	TTATAA	1 cut(s)	741
PspEI	GGTNACC	1 cut(s)	455
PspN4I	GGNNCC	3 cut(s)	174, 412, 433
PspPI	GGNCC	1 cut(s)	321
PstI	CTGCAG	1 cut(s)	165
PstNI	CAGNNNCTG	1 cut(s)	169
PsuI	RGATCY	2 cut(s)	364, 371
PvuII	CAGCTG	1 cut(s)	169
RsaI	GTAC	2 cut(s)	90, 353
RsaNI	GTAC	2 cut(s)	89, 352
RseI	CAYNNNNRTG	2 cut(s)	266, 316
SaqAI	TTAA	2 cut(s)	831, 842
SatI	GCNGC	4 cut(s)	161, 164, 167, 303
Sau3AI	GATC	4 cut(s)	364, 371, 565, 819
Sau96I	GGNCC	1 cut(s)	321
SchI	GAGTC	1 cut(s)	223
ScrFI	CCNGG	1 cut(s)	157
SduI	GDGCHC	5 cut(s)	263, 273, 330, 436, 640
SfaNI	GCATC	1 cut(s)	189
SfcI	CTRYAG	1 cut(s)	161
SmiMI	CAYNNNNRTG	2 cut(s)	266, 316
Sse9I	AATT	5 cut(s)	423, 491, 502, 623, 916
SsiI	CCGC	4 cut(s)	111, 255, 296, 362
SspMI	CTAG	2 cut(s)	281, 849
StyD4I	CCNGG	1 cut(s)	155
StyI	CCWWGG	4 cut(s)	451, 470, 513, 848
TaaI	ACNGT	4 cut(s)	88, 239, 268, 538
TaiI	ACGT	2 cut(s)	230, 357
TaqI	TCGA	1 cut(s)	147
TaqII	GACCGA	1 cut(s)	696
TasI	AATT	5 cut(s)	423, 491, 502, 623, 916
TfiI	GAWTC	1 cut(s)	686
Tru1I	TTAA	2 cut(s)	831, 842
Tru9I	TTAA	2 cut(s)	831, 842
TscAI	CASTG	1 cut(s)	543
TseFI	GTSAC	1 cut(s)	455
TseI	GCWGC	4 cut(s)	160, 163, 166, 302
Tsp45I	GTSAC	1 cut(s)	455
TspDTI	ATGAA	2 cut(s)	566, 617
TspGWI	ACGGA	1 cut(s)	280
TspRI	CASTG	1 cut(s)	543
Van91I	CCANNNNNTGG	1 cut(s)	428
VneI	GTGCAC	1 cut(s)	259
XceI	RCATGY	1 cut(s)	512
XmaJI	CCTAGG	1 cut(s)	848
XmiI	GTMKAC	1 cut(s)	217
XspI	CTAG	2 cut(s)	281, 849

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.