RchiOBHm_Chr5g0060431

Pentatricopeptide repeat-containing protein At2g27800, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0005837
Physical Location & Seq
Forward (+)
65566304 .. 65571997
5694 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1326 bp
ATGATTTCTCTTCGTAGATATTGCTGCAGAAATAGTTGCATGCTCAAAAACCTCAATAACAAGCTCTTTTGTGAACACCCTTTTCATCAAGACTTGTCCAACCTGAAGAGCATGGATGCTCATTCATATAGTTTGAATCCCATTAATAGTACTCTGAGGCCAATACACTATTTGACTGAACCCCACTTTGACATAGTACCCAAAGTTGCTTCCAATGGATTTCTTGGTATATACAAGCGATTTTCATTGCATAAGTTTTACTCATCCCCATCCCGATCTTTTAGAAGGAGAGAAAATAAAAGATTAGAATCCAGTAGAAAACCAACCCTTAATGAAGCCCAATTTCAAAGGTCTGTATCCCAGTTGTTGCCTAGATTTACACCTGAAGAACTATGCGATGTTATAACTAAGCAAGATGATCCTCTTGTGTGTTTAGAGTTGTTCAATTGGGCATCGCAACAGTCGCGGTTCAAACATGATGTTAACAGCTATCACATTACAATAAAGAAACTTGGTGTAGCTAAGATGTACGAAGAGATGGATGACATTGTCAACCAAGTGCTTGCTATCTCTCACATGGGTTCTGAGGCGCTTTTCAATTCCATTATCTACTTTTTCACAGAAGCTCGGAAGTTGACTAGAGCTGTCAATATATTTAAACACATGAAGAATAGTAGAAACATGGACTGTAGGCCTTCAGTTAGGACATACAATATTCTGTTTACTGCCTTTTTGAGTAGGGGATGTAATACCTACATAAATCACATGTATATGGAAACCATAAGATGTCTGTTTAGGCAAATGGTGAATGATGGGATTGAACCCGATATTTATACTTTGAATTCAATGATTAAGGGGTATGTTCTTTCCCTTCATGTGAATGATGCACTTAGAGTATTCCATCAGATGGGTGTGGTCTATAACTGCCTGCCAAACACATTTTCCTATGATTATCTCATCCATGGATTATGTGCTCAAGGCCGAACAAACAATGCTAAGCAGTTGTGCAATGAAATGAAGACTAAAGGGTTTATCCCGAGTAGTAAATCCTACAACTCGCTTGTGAATGCTTTGGCTCTTAGTGGAGAGATTGAGGAGGCAGTGAAGTATTTGTGGGAGATGATTGAGAAGCGAAGGTCAACTGAATTCATTACTTATAAGACAGTACTTGATGAAATGTGCAGACAAGGACAGGTTGGACAGGCAATGAAGTTGCTAAAAGAATTTCAAGAGAAAGACATTCTGAGTGGGCATGCTTACAGGAAGCTCCGATATGTGCTTGAAGATGACTATGGAGATTCTGTTTCCCATACTCGATTGAGATAA

Protein Analysis

441

Amino Acids

51.53

Weight (kDa)

9.16

Isoelectric Point (pI)

52.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 198 - 244 2.5e-06 PPR repeat family
PPR_2 PF13041 252 - 287 4.6e-06 PPR repeat family
PPR_3 PF13812 264 - 321 3.3e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 281 - 324 1.4e-07 PPR repeat family
PPR_1 PF12854 308 - 340 1.6e-08 PPR repeat
PPR_2 PF13041 311 - 359 1.7e-12 PPR repeat family
TPR_24 PF23276 313 - 409 9.9e-07 Fungal tetratrico peptide repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 404, 1158
AccB7I CCANNNNNTGG 1 cut(s) 907
AccII CGCG 1 cut(s) 466
AciI CCGC 1 cut(s) 466
AclWI GGATC 1 cut(s) 413
AcsI RAATTY 3 cut(s) 841, 1145, 1223
AcuI CTGAAG 3 cut(s) 125, 405, 681
AfaI GTAC 4 cut(s) 151, 198, 530, 1167
AfiI CCNNNNNNNGG 1 cut(s) 907
AflIII ACRYGT 1 cut(s) 765
AjuI GAANNNNNNNTTGG 2 cut(s) 925, 957
AluBI AGCT 6 cut(s) 64, 489, 521, 626, 644, 1267
AluI AGCT 6 cut(s) 64, 489, 521, 626, 644, 1267
Alw21I GWGCWC 1 cut(s) 976
AlwI GGATC 1 cut(s) 413
Ama87I CYCGRG 1 cut(s) 1036
AoxI GGCC 3 cut(s) 158, 692, 979
ApeKI GCWGC 1 cut(s) 24
ApoI RAATTY 3 cut(s) 841, 1145, 1223
AseI ATTAAT 1 cut(s) 144
AspLEI GCGC 1 cut(s) 592
AsuHPI GGTGA 1 cut(s) 817
AvaI CYCGRG 1 cut(s) 1036
BbsI GAAGAC 1 cut(s) 1025
Bbv12I GWGCWC 1 cut(s) 976
BbvI GCAGC 1 cut(s) 11
BccI CCATC 5 cut(s) 277, 532, 806, 901, 909
BciVI GTATCC 1 cut(s) 367
BfaI CTAG 2 cut(s) 372, 639
BfmI CTRYAG 2 cut(s) 25, 688
BfoI RGCGCY 1 cut(s) 593
BfuI GTATCC 1 cut(s) 367
BisI GCNGC 1 cut(s) 25
BlpI GCTNAGC 1 cut(s) 996
BlsI GCNGC 1 cut(s) 26
BmcAI AGTACT 2 cut(s) 151, 1167
BmeT110I CYCGRG 1 cut(s) 1036
BmrI ACTGGG 1 cut(s) 355
BmsI GCATC 3 cut(s) 106, 461, 874
BmuI ACTGGG 1 cut(s) 355
BpiI GAAGAC 1 cut(s) 1025
Bpu1102I GCTNAGC 1 cut(s) 996
BpuEI CTTGAG 1 cut(s) 960
BsaBI GATNNNNATC 1 cut(s) 307
BsaJI CCNNGG 1 cut(s) 961
BsaXI ACNNNNNCTCC 2 cut(s) 1287, 1317
Bsc4I CCNNNNNNNGG 1 cut(s) 907
Bse1I ACTGG 2 cut(s) 312, 361
Bse3DI GCAATG 3 cut(s) 245, 1015, 1212
Bse8I GATNNNNATC 1 cut(s) 307
BseDI CCNNGG 1 cut(s) 961
BseGI GGATG 6 cut(s) 121, 263, 269, 547, 749, 957
BseJI GATNNNNATC 1 cut(s) 307
BseLI CCNNNNNNNGG 1 cut(s) 907
BseMI GCAATG 3 cut(s) 245, 1015, 1212
BseMII CTCAG 3 cut(s) 146, 576, 1235
BseNI ACTGG 2 cut(s) 312, 361
BseRI GAGGAG 1 cut(s) 1109
BseXI GCAGC 1 cut(s) 11
BsgI GTGCAG 1 cut(s) 1201
Bsh1236I CGCG 1 cut(s) 466
BshFI GGCC 3 cut(s) 160, 694, 981
BsiHKAI GWGCWC 1 cut(s) 976
BsiHKCI CYCGRG 1 cut(s) 1036
BslI CCNNNNNNNGG 1 cut(s) 907
BsmI GAATGC 1 cut(s) 1072
BsnI GGCC 3 cut(s) 160, 694, 981
BsoBI CYCGRG 1 cut(s) 1036
Bsp1286I GDGCHC 1 cut(s) 976
Bsp143I GATC 2 cut(s) 275, 418
Bsp1720I GCTNAGC 1 cut(s) 996
Bsp19I CCATGG 1 cut(s) 961
BspACI CCGC 1 cut(s) 466
BspANI GGCC 3 cut(s) 160, 694, 981
BspCNI CTCAG 3 cut(s) 147, 577, 1236
BspFNI CGCG 1 cut(s) 466
BspMAI CTGCAG 1 cut(s) 29
BspPI GGATC 1 cut(s) 413
BspQI GCTCTTC 1 cut(s) 101
BsrDI GCAATG 3 cut(s) 245, 1015, 1212
BsrI ACTGG 2 cut(s) 312, 361
BssECI CCNNGG 1 cut(s) 961
BssMI GATC 2 cut(s) 275, 418
BssT1I CCWWGG 1 cut(s) 961
Bst4CI ACNGT 3 cut(s) 462, 689, 1165
Bst6I CTCTTC 3 cut(s) 15, 101, 528
BstC8I GCNNGC 4 cut(s) 41, 564, 929, 1254
BstDEI CTNAG 8 cut(s) 155, 408, 522, 585, 890, 996, 1079, 1244
BstDSI CCRYGG 1 cut(s) 961
BstF5I GGATG 6 cut(s) 121, 263, 269, 547, 749, 957
BstFNI CGCG 1 cut(s) 466
BstH2I RGCGCY 1 cut(s) 593
BstHHI GCGC 1 cut(s) 592
BstKTI GATC 2 cut(s) 278, 421
BstMBI GATC 2 cut(s) 275, 418
BstMWI GCNNNNNNNGC 1 cut(s) 463
BstNSI RCATGY 3 cut(s) 43, 769, 1256
BstSFI CTRYAG 2 cut(s) 25, 688
BstUI CGCG 1 cut(s) 466
BstV1I GCAGC 1 cut(s) 11
BstV2I GAAGAC 1 cut(s) 1025
BsuI GTATCC 1 cut(s) 367
BsuRI GGCC 3 cut(s) 160, 694, 981
BtgI CCRYGG 1 cut(s) 961
BtgZI GCGATG 2 cut(s) 411, 438
BtsCI GGATG 6 cut(s) 121, 263, 269, 547, 749, 957
BtsI GCAGTG 1 cut(s) 1107
BtsIMutI CAGTG 1 cut(s) 1107
Cac8I GCNNGC 4 cut(s) 41, 564, 929, 1254
CfoI GCGC 1 cut(s) 592
Csp6I GTAC 4 cut(s) 150, 197, 529, 1166
CviQI GTAC 4 cut(s) 150, 197, 529, 1166
DdeI CTNAG 8 cut(s) 155, 408, 522, 585, 890, 996, 1079, 1244
DpnI GATC 2 cut(s) 277, 420
DpnII GATC 2 cut(s) 275, 418
DraI TTTAAA 1 cut(s) 658
Eam1104I CTCTTC 3 cut(s) 15, 101, 528
EarI CTCTTC 3 cut(s) 15, 101, 528
Eco130I CCWWGG 1 cut(s) 961
Eco147I AGGCCT 1 cut(s) 694
Eco57I CTGAAG 3 cut(s) 125, 405, 681
Eco88I CYCGRG 1 cut(s) 1036
EcoRI GAATTC 2 cut(s) 841, 1145
EcoT14I CCWWGG 1 cut(s) 961
ErhI CCWWGG 1 cut(s) 961
Fnu4HI GCNGC 1 cut(s) 25
FokI GGATG 6 cut(s) 128, 250, 256, 554, 756, 944
Fsp4HI GCNGC 1 cut(s) 25
FspBI CTAG 2 cut(s) 372, 639
GlaI GCGC 1 cut(s) 591
GluI GCNGC 1 cut(s) 25
HaeII RGCGCY 1 cut(s) 593
HaeIII GGCC 3 cut(s) 160, 694, 981
HhaI GCGC 1 cut(s) 592
Hin6I GCGC 1 cut(s) 590
HinP1I GCGC 1 cut(s) 590
HincII GTYRAC 4 cut(s) 484, 553, 636, 1140
HindII GTYRAC 4 cut(s) 484, 553, 636, 1140
HinfI GANTC 3 cut(s) 136, 308, 1298
HpaI GTTAAC 1 cut(s) 484
HphI GGTGA 1 cut(s) 817
Hpy166II GTNNAC 6 cut(s) 74, 484, 553, 636, 723, 1140
Hpy188I TCNGA 6 cut(s) 156, 586, 630, 906, 1245, 1271
Hpy188III TCNNGA 4 cut(s) 89, 273, 1036, 1229
Hpy8I GTNNAC 6 cut(s) 74, 484, 553, 636, 723, 1140
HpyAV CCTTC 4 cut(s) 279, 705, 881, 1128
HpyCH4III ACNGT 3 cut(s) 462, 689, 1165
HpyCH4V TGCA 6 cut(s) 27, 39, 250, 887, 1008, 1182
HpyF10VI GCNNNNNNNGC 1 cut(s) 463
HpyF3I CTNAG 8 cut(s) 155, 408, 522, 585, 890, 996, 1079, 1244
HspAI GCGC 1 cut(s) 590
KspAI GTTAAC 1 cut(s) 484
Kzo9I GATC 2 cut(s) 275, 418
LguI GCTCTTC 1 cut(s) 101
LmnI GCTCC 1 cut(s) 1272
LpnPI CCDG 8 cut(s) 116, 325, 374, 396, 941, 1178, 1187, 1246
Lsp1109I GCAGC 1 cut(s) 11
LweI GCATC 3 cut(s) 106, 461, 874
MaeI CTAG 2 cut(s) 372, 639
MalI GATC 2 cut(s) 277, 420
MboI GATC 2 cut(s) 275, 418
MboII GAAGA 6 cut(s) 118, 398, 545, 679, 1030, 1295
MfeI CAATTG 1 cut(s) 445
MhlI GDGCHC 1 cut(s) 976
MluCI AATT 6 cut(s) 341, 445, 598, 841, 1145, 1223
MmeI TCCRAC 2 cut(s) 123, 1177
MnlI CCTC 6 cut(s) 62, 150, 432, 580, 1087, 1090
MseI TTAA 5 cut(s) 144, 330, 483, 657, 852
MslI CAYNNNNRTG 2 cut(s) 770, 879
MunI CAATTG 1 cut(s) 445
Mva1269I GAATGC 1 cut(s) 1072
MvnI CGCG 1 cut(s) 466
MwoI GCNNNNNNNGC 1 cut(s) 463
NcoI CCATGG 1 cut(s) 961
NdeII GATC 2 cut(s) 275, 418
NspI RCATGY 3 cut(s) 43, 769, 1256
PaeI GCATGC 2 cut(s) 43, 1256
PceI AGGCCT 1 cut(s) 694
PciI ACATGT 1 cut(s) 765
PciSI GCTCTTC 1 cut(s) 101
PctI GAATGC 1 cut(s) 1072
PfeI GAWTC 3 cut(s) 136, 308, 1298
PflFI GACNNNGTC 1 cut(s) 548
PflMI CCANNNNNTGG 1 cut(s) 907
PkrI GCNGC 1 cut(s) 26
PscI ACATGT 1 cut(s) 765
PshBI ATTAAT 1 cut(s) 144
PsiI TTATAA 2 cut(s) 404, 1158
PstI CTGCAG 1 cut(s) 29
PsyI GACNNNGTC 1 cut(s) 548
RsaI GTAC 4 cut(s) 151, 198, 530, 1167
RsaNI GTAC 4 cut(s) 150, 197, 529, 1166
RseI CAYNNNNRTG 2 cut(s) 770, 879
SapI GCTCTTC 1 cut(s) 101
SaqAI TTAA 5 cut(s) 144, 330, 483, 657, 852
SatI GCNGC 1 cut(s) 25
Sau3AI GATC 2 cut(s) 275, 418
ScaI AGTACT 2 cut(s) 151, 1167
SduI GDGCHC 1 cut(s) 976
SfaNI GCATC 3 cut(s) 106, 461, 874
SfcI CTRYAG 2 cut(s) 25, 688
SmiMI CAYNNNNRTG 2 cut(s) 770, 879
SmlI CTYRAG 1 cut(s) 975
SmoI CTYRAG 1 cut(s) 975
SphI GCATGC 2 cut(s) 43, 1256
Sse9I AATT 6 cut(s) 341, 445, 598, 841, 1145, 1223
SseBI AGGCCT 1 cut(s) 694
SsiI CCGC 1 cut(s) 466
SspI AATATT 1 cut(s) 715
SspMI CTAG 2 cut(s) 372, 639
StuI AGGCCT 1 cut(s) 694
StyI CCWWGG 1 cut(s) 961
TaaI ACNGT 3 cut(s) 462, 689, 1165
TaqI TCGA 1 cut(s) 1315
TasI AATT 6 cut(s) 341, 445, 598, 841, 1145, 1223
TatI WGTACW 2 cut(s) 149, 1165
TfiI GAWTC 3 cut(s) 136, 308, 1298
Tru1I TTAA 5 cut(s) 144, 330, 483, 657, 852
Tru9I TTAA 5 cut(s) 144, 330, 483, 657, 852
TscAI CASTG 1 cut(s) 1107
TseI GCWGC 1 cut(s) 24
TspRI CASTG 1 cut(s) 1107
Tth111I GACNNNGTC 1 cut(s) 548
Van91I CCANNNNNTGG 1 cut(s) 907
VspI ATTAAT 1 cut(s) 144
XapI RAATTY 3 cut(s) 841, 1145, 1223
XceI RCATGY 3 cut(s) 43, 769, 1256
XspI CTAG 2 cut(s) 372, 639
ZrmI AGTACT 2 cut(s) 151, 1167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.