RchiOBHm_Chr7g0223131

Pentatricopeptide repeat-containing protein At2g27800, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0005837
Physical Location & Seq
Forward (+)
44766401 .. 44770267
3867 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19978

Sequence Viewer

Length: 750 bp
ATGGGTTCTGTGGCGCTTTTCAATTCCATTATCTACTTTTTCACAGAAGCTCGGAAGTTGACTAGAGCTGTCAATATATTTAAACACATGAAGAATAGTAGAAACATGGACTGTAGGCCTTCTGTTAGGACATACAATATTCTGTTTACTGCCTTTTTGAGTAGGGGATGTAATACCTACATAAATCACATGTATATGGAAACCATAAGATGTCTGTTTAGGCAAATGGTGAATGATGGGATTGAACCCGATATTTATACTTTGAATTCAATGATTAAGGGGTATGTTCTTTCCCTTCATGTGAATGATGCACTTAGAGTATTCCATCAGATGGGTGTGGTCTATAACTGCCTGCCAAACACATTTTCCTATGATTATCTCATCCATGGATTATGTGCTCAAGGCCGAACAAACAATGCTAAGCAGTTGTGCAACGAAATGAAGACTAAAGGGTTTATCCCGAGTAGTAAATCCTACAACTCACTTGTGAATGCTTTGGCTCTTTGTGGAGAGATTGAGGAGGCAGTGAAGTATTTGTGGGAGATGATTGAGAAGCGAAGGTCAACTGAATTCATTACTTATAAGACAGTACTTGATGAAATGTGCAGACAAGGACAGGTTGGACAAGCAATGAAGTTGCTAAAAGAATTTCAAAAGAAAGACATTCTGAGTGGGCATGCTTACAGGAAGCTCCGATATGTGCTTGAAGATGACTATGGAGATTCTGTCTCCCATACTCGATTGAGATAA

Protein Analysis

249

Amino Acids

28.97

Weight (kDa)

9.19

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 3 - 52 3.7e-07 PPR repeat family
PPR_long PF17177 18 - 187 1.3e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 60 - 95 2.1e-06 PPR repeat family
PPR_3 PF13812 72 - 129 1.3e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 89 - 132 6.1e-08 PPR repeat family
PPR_1 PF12854 116 - 148 7.3e-09 PPR repeat
PPR_2 PF13041 119 - 167 7.2e-13 PPR repeat family
TPR_24 PF23276 121 - 217 9.2e-07 Fungal tetratrico peptide repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 582
AccB7I CCANNNNNTGG 1 cut(s) 331
AcsI RAATTY 3 cut(s) 265, 569, 647
AfaI GTAC 1 cut(s) 591
AfiI CCNNNNNNNGG 1 cut(s) 331
AflIII ACRYGT 1 cut(s) 189
AgsI TTSAA 6 cut(s) 22, 245, 265, 270, 653, 707
AjuI GAANNNNNNNTTGG 2 cut(s) 349, 381
AluBI AGCT 3 cut(s) 50, 68, 691
AluI AGCT 3 cut(s) 50, 68, 691
Alw21I GWGCWC 1 cut(s) 400
Alw26I GTCTC 1 cut(s) 733
Ama87I CYCGRG 1 cut(s) 460
AoxI GGCC 2 cut(s) 116, 403
ApoI RAATTY 3 cut(s) 265, 569, 647
AspLEI GCGC 1 cut(s) 16
AsuHPI GGTGA 1 cut(s) 241
AvaI CYCGRG 1 cut(s) 460
BbsI GAAGAC 1 cut(s) 449
Bbv12I GWGCWC 1 cut(s) 400
BccI CCATC 3 cut(s) 230, 325, 333
BcoDI GTCTC 1 cut(s) 733
BfaI CTAG 1 cut(s) 63
BfmI CTRYAG 1 cut(s) 112
BfoI RGCGCY 1 cut(s) 17
BlpI GCTNAGC 1 cut(s) 420
BmcAI AGTACT 1 cut(s) 591
BmeT110I CYCGRG 1 cut(s) 460
BmsI GCATC 1 cut(s) 298
BpiI GAAGAC 1 cut(s) 449
Bpu1102I GCTNAGC 1 cut(s) 420
BpuEI CTTGAG 1 cut(s) 384
BsaJI CCNNGG 1 cut(s) 385
BsaXI ACNNNNNCTCC 2 cut(s) 711, 741
Bsc4I CCNNNNNNNGG 1 cut(s) 331
Bse3DI GCAATG 1 cut(s) 636
BseDI CCNNGG 1 cut(s) 385
BseGI GGATG 2 cut(s) 173, 381
BseLI CCNNNNNNNGG 1 cut(s) 331
BseMI GCAATG 1 cut(s) 636
BseMII CTCAG 1 cut(s) 659
BseRI GAGGAG 1 cut(s) 533
BsgI GTGCAG 1 cut(s) 625
BshFI GGCC 2 cut(s) 118, 405
BsiHKAI GWGCWC 1 cut(s) 400
BsiHKCI CYCGRG 1 cut(s) 460
BslI CCNNNNNNNGG 1 cut(s) 331
BsmAI GTCTC 1 cut(s) 733
BsmI GAATGC 1 cut(s) 496
BsnI GGCC 2 cut(s) 118, 405
BsoBI CYCGRG 1 cut(s) 460
Bsp1286I GDGCHC 1 cut(s) 400
Bsp1720I GCTNAGC 1 cut(s) 420
Bsp19I CCATGG 1 cut(s) 385
BspANI GGCC 2 cut(s) 118, 405
BspCNI CTCAG 1 cut(s) 660
BsrDI GCAATG 1 cut(s) 636
BssECI CCNNGG 1 cut(s) 385
BssT1I CCWWGG 1 cut(s) 385
Bst4CI ACNGT 2 cut(s) 113, 589
BstC8I GCNNGC 2 cut(s) 353, 678
BstDEI CTNAG 3 cut(s) 314, 420, 668
BstDSI CCRYGG 1 cut(s) 385
BstF5I GGATG 2 cut(s) 173, 381
BstH2I RGCGCY 1 cut(s) 17
BstHHI GCGC 1 cut(s) 16
BstMAI GTCTC 1 cut(s) 733
BstNSI RCATGY 2 cut(s) 193, 680
BstSFI CTRYAG 1 cut(s) 112
BstV2I GAAGAC 1 cut(s) 449
BsuRI GGCC 2 cut(s) 118, 405
BtgI CCRYGG 1 cut(s) 385
BtsCI GGATG 2 cut(s) 173, 381
BtsI GCAGTG 1 cut(s) 531
BtsIMutI CAGTG 1 cut(s) 531
Cac8I GCNNGC 2 cut(s) 353, 678
CfoI GCGC 1 cut(s) 16
Csp6I GTAC 1 cut(s) 590
CviAII CATG 6 cut(s) 88, 106, 190, 299, 386, 677
CviJI RGCY 6 cut(s) 50, 68, 118, 405, 500, 691
CviKI_1 RGCY 6 cut(s) 50, 68, 118, 405, 500, 691
CviQI GTAC 1 cut(s) 590
DdeI CTNAG 3 cut(s) 314, 420, 668
DraI TTTAAA 1 cut(s) 82
Eco130I CCWWGG 1 cut(s) 385
Eco147I AGGCCT 1 cut(s) 118
Eco88I CYCGRG 1 cut(s) 460
EcoRI GAATTC 2 cut(s) 265, 569
EcoT14I CCWWGG 1 cut(s) 385
ErhI CCWWGG 1 cut(s) 385
FaeI CATG 6 cut(s) 91, 109, 193, 302, 389, 680
FatI CATG 6 cut(s) 87, 105, 189, 298, 385, 676
FokI GGATG 2 cut(s) 180, 368
FspBI CTAG 1 cut(s) 63
GlaI GCGC 1 cut(s) 15
HaeII RGCGCY 1 cut(s) 17
HaeIII GGCC 2 cut(s) 118, 405
HhaI GCGC 1 cut(s) 16
Hin1II CATG 6 cut(s) 91, 109, 193, 302, 389, 680
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HincII GTYRAC 2 cut(s) 60, 564
HindII GTYRAC 2 cut(s) 60, 564
HinfI GANTC 1 cut(s) 722
HphI GGTGA 1 cut(s) 241
Hpy166II GTNNAC 3 cut(s) 60, 147, 564
Hpy188I TCNGA 4 cut(s) 54, 330, 669, 695
Hpy188III TCNNGA 1 cut(s) 460
Hpy8I GTNNAC 3 cut(s) 60, 147, 564
HpyAV CCTTC 3 cut(s) 129, 305, 552
HpyCH4III ACNGT 2 cut(s) 113, 589
HpyCH4V TGCA 3 cut(s) 311, 432, 606
HpyF3I CTNAG 3 cut(s) 314, 420, 668
Hsp92II CATG 6 cut(s) 91, 109, 193, 302, 389, 680
HspAI GCGC 1 cut(s) 14
LmnI GCTCC 1 cut(s) 696
LpnPI CCDG 3 cut(s) 365, 602, 670
LweI GCATC 1 cut(s) 298
MaeI CTAG 1 cut(s) 63
MboII GAAGA 3 cut(s) 103, 454, 719
MhlI GDGCHC 1 cut(s) 400
MluCI AATT 4 cut(s) 22, 265, 569, 647
MmeI TCCRAC 1 cut(s) 601
MnlI CCTC 2 cut(s) 511, 514
MseI TTAA 2 cut(s) 81, 276
MslI CAYNNNNRTG 2 cut(s) 194, 303
Mva1269I GAATGC 1 cut(s) 496
NcoI CCATGG 1 cut(s) 385
NlaIII CATG 6 cut(s) 91, 109, 193, 302, 389, 680
NspI RCATGY 2 cut(s) 193, 680
PaeI GCATGC 1 cut(s) 680
PceI AGGCCT 1 cut(s) 118
PciI ACATGT 1 cut(s) 189
PctI GAATGC 1 cut(s) 496
PfeI GAWTC 1 cut(s) 722
PflMI CCANNNNNTGG 1 cut(s) 331
PscI ACATGT 1 cut(s) 189
PsiI TTATAA 1 cut(s) 582
RsaI GTAC 1 cut(s) 591
RsaNI GTAC 1 cut(s) 590
RseI CAYNNNNRTG 2 cut(s) 194, 303
SaqAI TTAA 2 cut(s) 81, 276
ScaI AGTACT 1 cut(s) 591
SduI GDGCHC 1 cut(s) 400
SetI ASST 6 cut(s) 52, 70, 179, 563, 621, 693
SfaNI GCATC 1 cut(s) 298
SfcI CTRYAG 1 cut(s) 112
SmiMI CAYNNNNRTG 2 cut(s) 194, 303
SmlI CTYRAG 1 cut(s) 399
SmoI CTYRAG 1 cut(s) 399
SphI GCATGC 1 cut(s) 680
Sse9I AATT 4 cut(s) 22, 265, 569, 647
SseBI AGGCCT 1 cut(s) 118
SspI AATATT 1 cut(s) 139
SspMI CTAG 1 cut(s) 63
StuI AGGCCT 1 cut(s) 118
StyI CCWWGG 1 cut(s) 385
TaaI ACNGT 2 cut(s) 113, 589
TaqI TCGA 1 cut(s) 739
TasI AATT 4 cut(s) 22, 265, 569, 647
TatI WGTACW 1 cut(s) 589
TfiI GAWTC 1 cut(s) 722
Tru1I TTAA 2 cut(s) 81, 276
Tru9I TTAA 2 cut(s) 81, 276
TscAI CASTG 1 cut(s) 531
TspDTI ATGAA 6 cut(s) 104, 287, 455, 562, 612, 647
TspRI CASTG 1 cut(s) 531
Van91I CCANNNNNTGG 1 cut(s) 331
XapI RAATTY 3 cut(s) 265, 569, 647
XceI RCATGY 2 cut(s) 193, 680
XspI CTAG 1 cut(s) 63
ZrmI AGTACT 1 cut(s) 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.