RchiOBHm_Chr5g0063801

Glyoxalase-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0013486
Physical Location & Seq
Forward (+)
69427974 .. 69428611
638 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 426 bp
ATGGCGTCGAATCTAAACCCAGCATTTGCCTACACGGTGGTGTACGTGAAGGACGTAGCCAAATCCGCAGCCTTCTATTCCAAAGCCTTCGGCTACGCCGTACGTCGTATAGACGAGTCTCACAGATGGGGAGAGCTTGAAAGCGGACAGACGACGATAGCTTTCACGCCGCAAAACCAGCACGAGACGGACGACCTCACCGGAAAAGTTCAGAAGCCGAGCTCCGGGAGTGAGAGGCAGCAGGTTGAGGTTTGCTTTGCGTACCCCGACGTCGACGCGGCCTATAAAAGGGCGGTGGAGAACGGAGCGGTGGCGGTCAGCGCGCCGGAAGAGAAAGAGTGGGGCCAGAAAGTGGGGTATGTACGAGATCCCGATGGGATCGTGGTGAGGATGGGAAGCTACGTCAACGAACCGAAGCAGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase Glyoxalase_2

Protein Analysis

141

Amino Acids

15.54

Weight (kDa)

5.41

Isoelectric Point (pI)

30.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 9 - 129 7.5e-14 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_2 PF12681 9 - 133 1.8e-10 Glyoxalase-like domain
Glyoxalase_6 PF18029 13 - 126 1e-11 Glyoxalase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013486)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07645
fragaria_vesca FvH4_3g35580 FvH4_3g35610
malus_domestica MD07G1053700.v1.1 MD11G1144900.v1.1 MD14G1025700.v1.1
prunus_persica Prupe.6G080800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0063801
rosa_laevigata RLG00000035673
rosa_multiflora Rmu_sc0005685.1_g000007 Rmu_ssc0000309.1_g000017
rosa_roxburghii Rroxscaffold_1G00017030
rosa_rugosa Rorug05G0360000 Rorug05G0360000
rosa_samantha Rh5BG434400 Rh5CG457600 Rh5DG447500
rosa_wichuraiana Rw5G039320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 273
Acc36I ACCTGC 1 cut(s) 232
AccB7I CCANNNNNTGG 1 cut(s) 352
AccBSI CCGCTC 1 cut(s) 308
AccI GTMKAC 1 cut(s) 273
AccII CGCG 2 cut(s) 278, 323
AciI CCGC 7 cut(s) 66, 144, 170, 278, 293, 308, 314
AclWI GGATC 2 cut(s) 362, 386
AcyI GRCGYC 2 cut(s) 5, 270
AfaI GTAC 4 cut(s) 44, 102, 263, 363
AfiI CCNNNNNNNGG 4 cut(s) 224, 288, 352, 419
AgsI TTSAA 1 cut(s) 140
AleI CACNNNNGTG 1 cut(s) 38
AluBI AGCT 4 cut(s) 136, 161, 222, 399
AluI AGCT 4 cut(s) 136, 161, 222, 399
Alw21I GWGCWC 1 cut(s) 224
Alw26I GTCTC 2 cut(s) 123, 179
AlwI GGATC 2 cut(s) 362, 386
AoxI GGCC 2 cut(s) 279, 343
ApeKI GCWGC 2 cut(s) 68, 238
AspLEI GCGC 2 cut(s) 323, 325
AspS9I GGNCC 1 cut(s) 343
AsuC2I CCSGG 1 cut(s) 226
AsuHPI GGTGA 2 cut(s) 190, 397
BanII GRGCYC 1 cut(s) 224
BauI CACGAG 1 cut(s) 182
Bbv12I GWGCWC 1 cut(s) 224
BbvI GCAGC 2 cut(s) 80, 250
BccI CCATC 3 cut(s) 120, 368, 385
BceAI ACGGC 1 cut(s) 83
BcnI CCSGG 1 cut(s) 226
BcoDI GTCTC 2 cut(s) 123, 179
BfuAI ACCTGC 1 cut(s) 232
BisI GCNGC 4 cut(s) 69, 170, 239, 279
BlsI GCNGC 4 cut(s) 70, 171, 240, 280
Bme1390I CCNGG 1 cut(s) 226
BmgT120I GGNCC 1 cut(s) 343
BmiI GGNNCC 1 cut(s) 344
BmrFI CCNGG 1 cut(s) 226
BpuMI CCSGG 1 cut(s) 226
BsaAI YACGTR 1 cut(s) 46
BsaHI GRCGYC 2 cut(s) 5, 270
BsaWI WCCGGW 1 cut(s) 200
Bsc4I CCNNNNNNNGG 4 cut(s) 224, 288, 352, 419
BseGI GGATG 1 cut(s) 396
BseLI CCNNNNNNNGG 4 cut(s) 224, 288, 352, 419
BsePI GCGCGC 1 cut(s) 321
BseXI GCAGC 2 cut(s) 80, 250
BseYI CCCAGC 1 cut(s) 19
Bsh1236I CGCG 2 cut(s) 278, 323
BshFI GGCC 2 cut(s) 281, 345
BsiHKAI GWGCWC 1 cut(s) 224
BsiSI CCGG 3 cut(s) 201, 225, 326
BsiWI CGTACG 1 cut(s) 100
BslI CCNNNNNNNGG 4 cut(s) 224, 288, 352, 419
BsmAI GTCTC 2 cut(s) 123, 179
BsmBI CGTCTC 1 cut(s) 179
BsnI GGCC 2 cut(s) 281, 345
Bsp1286I GDGCHC 1 cut(s) 224
Bsp143I GATC 2 cut(s) 367, 378
BspACI CCGC 7 cut(s) 66, 144, 170, 278, 293, 308, 314
BspANI GGCC 2 cut(s) 281, 345
BspFNI CGCG 2 cut(s) 278, 323
BspLI GGNNCC 1 cut(s) 344
BspMI ACCTGC 1 cut(s) 232
BspPI GGATC 2 cut(s) 362, 386
BsrBI CCGCTC 1 cut(s) 308
BssHII GCGCGC 1 cut(s) 321
BssMI GATC 2 cut(s) 367, 378
BssNI GRCGYC 2 cut(s) 5, 270
BssSI CACGAG 1 cut(s) 182
Bst2BI CACGAG 1 cut(s) 182
Bst4CI ACNGT 1 cut(s) 37
Bst6I CTCTTC 1 cut(s) 324
BstACI GRCGYC 2 cut(s) 5, 270
BstBAI YACGTR 1 cut(s) 46
BstC8I GCNNGC 1 cut(s) 323
BstENI CCTNNNNNAGG 1 cut(s) 286
BstF5I GGATG 1 cut(s) 396
BstFNI CGCG 2 cut(s) 278, 323
BstHHI GCGC 2 cut(s) 323, 325
BstKTI GATC 2 cut(s) 370, 381
BstMAI GTCTC 2 cut(s) 123, 179
BstMBI GATC 2 cut(s) 367, 378
BstMWI GCNNNNNNNGC 3 cut(s) 65, 178, 320
BstSCI CCNGG 1 cut(s) 224
BstUI CGCG 2 cut(s) 278, 323
BstV1I GCAGC 2 cut(s) 80, 250
BstX2I RGATCY 1 cut(s) 367
BstYI RGATCY 1 cut(s) 367
BsuRI GGCC 2 cut(s) 281, 345
BtsCI GGATG 1 cut(s) 396
BveI ACCTGC 1 cut(s) 232
Cac8I GCNNGC 1 cut(s) 323
CfoI GCGC 2 cut(s) 323, 325
Cfr13I GGNCC 1 cut(s) 343
CseI GACGC 1 cut(s) 284
Csp6I GTAC 4 cut(s) 43, 101, 262, 362
CviQI GTAC 4 cut(s) 43, 101, 262, 362
DpnI GATC 2 cut(s) 369, 380
DpnII GATC 2 cut(s) 367, 378
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
Ecl136II GAGCTC 1 cut(s) 222
Eco24I GRGCYC 1 cut(s) 224
Eco53kI GAGCTC 1 cut(s) 222
EcoICRI GAGCTC 1 cut(s) 222
EcoNI CCTNNNNNAGG 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 224
Esp3I CGTCTC 1 cut(s) 179
FaiI YATR 3 cut(s) 110, 285, 360
FblI GTMKAC 1 cut(s) 273
Fnu4HI GCNGC 4 cut(s) 69, 170, 239, 279
FokI GGATG 1 cut(s) 403
FriOI GRGCYC 1 cut(s) 224
Fsp4HI GCNGC 4 cut(s) 69, 170, 239, 279
GlaI GCGC 2 cut(s) 322, 324
GluI GCNGC 4 cut(s) 69, 170, 239, 279
GsaI CCCAGC 1 cut(s) 23
HaeIII GGCC 2 cut(s) 281, 345
HapII CCGG 3 cut(s) 201, 225, 326
HgaI GACGC 1 cut(s) 284
HhaI GCGC 2 cut(s) 323, 325
Hin1I GRCGYC 2 cut(s) 5, 270
Hin6I GCGC 2 cut(s) 321, 323
HinP1I GCGC 2 cut(s) 321, 323
HincII GTYRAC 2 cut(s) 274, 406
HindII GTYRAC 2 cut(s) 274, 406
HinfI GANTC 2 cut(s) 10, 116
HpaII CCGG 3 cut(s) 201, 225, 326
HphI GGTGA 2 cut(s) 190, 397
Hpy166II GTNNAC 3 cut(s) 43, 274, 406
Hpy188I TCNGA 1 cut(s) 213
Hpy188III TCNNGA 1 cut(s) 371
Hpy8I GTNNAC 3 cut(s) 43, 274, 406
Hpy99I CGWCG 6 cut(s) 10, 108, 157, 272, 275, 278
HpyAV CCTTC 3 cut(s) 43, 82, 97
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4IV ACGT 5 cut(s) 45, 54, 103, 270, 402
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 178, 320
HpySE526I ACGT 5 cut(s) 45, 54, 103, 270, 402
Hsp92I GRCGYC 2 cut(s) 5, 270
HspAI GCGC 2 cut(s) 321, 323
Kzo9I GATC 2 cut(s) 367, 378
LmnI GCTCC 2 cut(s) 227, 305
LpnPI CCDG 8 cut(s) 33, 191, 214, 227, 238, 339, 359, 404
Lsp1109I GCAGC 2 cut(s) 80, 250
MaeII ACGT 5 cut(s) 45, 54, 103, 270, 402
MalI GATC 2 cut(s) 369, 380
MbiI CCGCTC 1 cut(s) 308
MboI GATC 2 cut(s) 367, 378
MboII GAAGA 1 cut(s) 341
MflI RGATCY 1 cut(s) 367
MhlI GDGCHC 1 cut(s) 224
MlyI GAGTC 1 cut(s) 125
MnlI CCTC 4 cut(s) 206, 228, 241, 381
MseI TTAA 1 cut(s) 424
MslI CAYNNNNRTG 1 cut(s) 38
MspI CCGG 3 cut(s) 201, 225, 326
MspR9I CCNGG 1 cut(s) 226
MvnI CGCG 2 cut(s) 278, 323
MwoI GCNNNNNNNGC 3 cut(s) 65, 178, 320
NciI CCSGG 1 cut(s) 226
NdeII GATC 2 cut(s) 367, 378
NlaIV GGNNCC 1 cut(s) 344
NmeAIII GCCGAG 1 cut(s) 243
OliI CACNNNNGTG 1 cut(s) 38
PauI GCGCGC 1 cut(s) 321
PcsI WCGNNNNNNNCGW 3 cut(s) 51, 96, 189
PfeI GAWTC 1 cut(s) 10
Pfl23II CGTACG 1 cut(s) 100
PflMI CCANNNNNTGG 1 cut(s) 352
PfoI TCCNGGA 1 cut(s) 224
PkrI GCNGC 4 cut(s) 70, 171, 240, 280
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
Ppu21I YACGTR 1 cut(s) 46
Psp124BI GAGCTC 1 cut(s) 224
PspFI CCCAGC 1 cut(s) 19
PspLI CGTACG 1 cut(s) 100
PspN4I GGNNCC 1 cut(s) 344
PspPI GGNCC 1 cut(s) 343
PsuI RGATCY 1 cut(s) 367
PteI GCGCGC 1 cut(s) 321
RsaI GTAC 4 cut(s) 44, 102, 263, 363
RsaNI GTAC 4 cut(s) 43, 101, 262, 362
RseI CAYNNNNRTG 1 cut(s) 38
SacI GAGCTC 1 cut(s) 224
SalI GTCGAC 1 cut(s) 272
SaqAI TTAA 1 cut(s) 424
SatI GCNGC 4 cut(s) 69, 170, 239, 279
Sau3AI GATC 2 cut(s) 367, 378
Sau96I GGNCC 1 cut(s) 343
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 1 cut(s) 226
SduI GDGCHC 1 cut(s) 224
SgrDI CGTCGACG 1 cut(s) 272
SmiMI CAYNNNNRTG 1 cut(s) 38
SsiI CCGC 7 cut(s) 66, 144, 170, 278, 293, 308, 314
SstI GAGCTC 1 cut(s) 224
StyD4I CCNGG 1 cut(s) 224
TaaI ACNGT 1 cut(s) 37
TaiI ACGT 5 cut(s) 48, 57, 106, 273, 405
TaqI TCGA 2 cut(s) 8, 273
TauI GCSGC 2 cut(s) 172, 281
TfiI GAWTC 1 cut(s) 10
Tru1I TTAA 1 cut(s) 424
Tru9I TTAA 1 cut(s) 424
TseI GCWGC 2 cut(s) 68, 238
TspGWI ACGGA 2 cut(s) 203, 318
Van91I CCANNNNNTGG 1 cut(s) 352
XagI CCTNNNNNAGG 1 cut(s) 286
XmiI GTMKAC 1 cut(s) 273
ZraI GACGTC 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.