Rmu_sc0005685.1_g000007

Glyoxalase-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005685.1
OG0013486
Physical Location & Seq
Reverse (-)
33671 .. 35797
2127 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005685.1_g000007.1.cds

Sequence Viewer

Length: 864 bp
atggcgtcgaatctaaacccagcatttgcctacacggtggtgttcgtgaaggacgtagccaaatccgcagccttctattccaaagccttcggctacgccgtacgtcgtatagacgagtctcacagatggggagagcttgaaagcgggcagacgacgatagctttcacgccgcaaaaccagcacgagacggacgacctcaccggaaaagttcagaagccgagctccgggagtgagaggcagccggttgaggtttgctttgcgtaccccgacgtcgacgcggcctataaaagggcggtggagaacggagcggtggcggtgagcgcgccggaagagaaagagtggggccagaaagtggggtatgtacgagatcccgatgggatcgtgttgaataatagaaagaagcaaaggaaccgtttggcatatcagaagttggaaaagcttgtatactgctactataacatgaagctgtggctgcgagatagacatgcaatgatgaatgtggtcaatgaaaacaactatatagatctacttcatgttgcaagtgagccgcttgataatggcattgatccacttcattattggattatgcctgcacacctcgatgatgaagctggaaaacctcttccacaagttgtagaaactgcaactaatactgtaatcaacatagagaaggtcttatatgaagaagttgttaaaaacccaggagataattcagatagtgatgatgcgtggggtggtacagtaactccagatagttctgatgatggtggagagggtggaagtggaacaggtggtggaggtgaaagatatgaatatggacaatcttctatgcatagaggattaaacccatttacaggcctgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase Glyoxalase_2

Protein Analysis

287

Amino Acids

31.81

Weight (kDa)

5.01

Isoelectric Point (pI)

35.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013486)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07645
fragaria_vesca FvH4_3g35580 FvH4_3g35610
malus_domestica MD07G1053700.v1.1 MD11G1144900.v1.1 MD14G1025700.v1.1
prunus_persica Prupe.6G080800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0063801
rosa_laevigata RLG00000035673
rosa_multiflora Rmu_sc0005685.1_g000007 Rmu_ssc0000309.1_g000017
rosa_roxburghii Rroxscaffold_1G00017030
rosa_rugosa Rorug05G0360000 Rorug05G0360000
rosa_samantha Rh5BG434400 Rh5CG457600 Rh5DG447500
rosa_wichuraiana Rw5G039320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 273
AccB7I CCANNNNNTGG 1 cut(s) 352
AccBSI CCGCTC 1 cut(s) 308
AccI GTMKAC 2 cut(s) 273, 444
AccII CGCG 2 cut(s) 278, 323
AciI CCGC 8 cut(s) 66, 144, 170, 278, 293, 308, 314, 548
AclWI GGATC 3 cut(s) 362, 386, 560
AcyI GRCGYC 2 cut(s) 5, 270
AfaI GTAC 4 cut(s) 102, 263, 363, 739
AfiI CCNNNNNNNGG 4 cut(s) 224, 288, 352, 854
AgsI TTSAA 2 cut(s) 140, 388
AjnI CCWGG 1 cut(s) 700
AleI CACNNNNGTG 1 cut(s) 38
AluBI AGCT 6 cut(s) 136, 161, 222, 439, 466, 611
AluI AGCT 6 cut(s) 136, 161, 222, 439, 466, 611
Alw21I GWGCWC 1 cut(s) 224
Alw26I GTCTC 2 cut(s) 123, 179
AlwI GGATC 3 cut(s) 362, 386, 560
AoxI GGCC 3 cut(s) 279, 343, 856
ApeKI GCWGC 3 cut(s) 68, 238, 472
AspLEI GCGC 2 cut(s) 323, 325
AspS9I GGNCC 1 cut(s) 343
AsuC2I CCSGG 1 cut(s) 226
AsuHPI GGTGA 3 cut(s) 190, 328, 812
BanII GRGCYC 1 cut(s) 224
BauI CACGAG 1 cut(s) 182
Bbv12I GWGCWC 1 cut(s) 224
BbvI GCAGC 3 cut(s) 80, 250, 459
BccI CCATC 3 cut(s) 120, 368, 758
BceAI ACGGC 1 cut(s) 83
BciT130I CCWGG 1 cut(s) 702
BcnI CCSGG 1 cut(s) 226
BcoDI GTCTC 2 cut(s) 123, 179
BglII AGATCT 1 cut(s) 523
BisI GCNGC 6 cut(s) 69, 170, 239, 279, 473, 548
BlsI GCNGC 6 cut(s) 70, 171, 240, 280, 474, 549
Bme1390I CCNGG 2 cut(s) 226, 702
BmgT120I GGNCC 1 cut(s) 343
BmiI GGNNCC 2 cut(s) 344, 410
BmrFI CCNGG 2 cut(s) 226, 702
BmsI GCATC 1 cut(s) 715
BpmI CTGGAG 1 cut(s) 732
BpuMI CCSGG 1 cut(s) 226
BsaHI GRCGYC 2 cut(s) 5, 270
BsaJI CCNNGG 1 cut(s) 700
BsaWI WCCGGW 1 cut(s) 200
BsaXI ACNNNNNCTCC 2 cut(s) 730, 760
Bsc4I CCNNNNNNNGG 4 cut(s) 224, 288, 352, 854
Bse118I RCCGGY 1 cut(s) 241
Bse3DI GCAATG 1 cut(s) 495
BseBI CCWGG 1 cut(s) 702
BseDI CCNNGG 1 cut(s) 700
BseLI CCNNNNNNNGG 4 cut(s) 224, 288, 352, 854
BseMI GCAATG 1 cut(s) 495
BsePI GCGCGC 1 cut(s) 321
BseXI GCAGC 3 cut(s) 80, 250, 459
BseYI CCCAGC 1 cut(s) 19
BsgI GTGCAG 1 cut(s) 576
Bsh1236I CGCG 2 cut(s) 278, 323
BshFI GGCC 3 cut(s) 281, 345, 858
BsiHKAI GWGCWC 1 cut(s) 224
BsiSI CCGG 4 cut(s) 201, 225, 242, 326
BsiWI CGTACG 1 cut(s) 100
BslI CCNNNNNNNGG 4 cut(s) 224, 288, 352, 854
BsmAI GTCTC 2 cut(s) 123, 179
BsmBI CGTCTC 1 cut(s) 179
BsnI GGCC 3 cut(s) 281, 345, 858
Bsp1286I GDGCHC 1 cut(s) 224
Bsp143I GATC 4 cut(s) 367, 378, 523, 565
BspACI CCGC 8 cut(s) 66, 144, 170, 278, 293, 308, 314, 548
BspANI GGCC 3 cut(s) 281, 345, 858
BspFNI CGCG 2 cut(s) 278, 323
BspLI GGNNCC 2 cut(s) 344, 410
BspPI GGATC 3 cut(s) 362, 386, 560
BsrBI CCGCTC 1 cut(s) 308
BsrDI GCAATG 1 cut(s) 495
BsrFI RCCGGY 1 cut(s) 241
BssAI RCCGGY 1 cut(s) 241
BssECI CCNNGG 1 cut(s) 700
BssHII GCGCGC 1 cut(s) 321
BssMI GATC 4 cut(s) 367, 378, 523, 565
BssNAI GTATAC 1 cut(s) 445
BssNI GRCGYC 2 cut(s) 5, 270
BssSI CACGAG 1 cut(s) 182
Bst1107I GTATAC 1 cut(s) 445
Bst2BI CACGAG 1 cut(s) 182
Bst2UI CCWGG 1 cut(s) 702
Bst4CI ACNGT 4 cut(s) 37, 413, 655, 742
Bst6I CTCTTC 2 cut(s) 324, 627
BstACI GRCGYC 2 cut(s) 5, 270
BstC8I GCNNGC 3 cut(s) 146, 323, 591
BstENI CCTNNNNNAGG 1 cut(s) 286
BstFNI CGCG 2 cut(s) 278, 323
BstHHI GCGC 2 cut(s) 323, 325
BstKTI GATC 4 cut(s) 370, 381, 526, 568
BstMAI GTCTC 2 cut(s) 123, 179
BstMBI GATC 4 cut(s) 367, 378, 523, 565
BstMWI GCNNNNNNNGC 4 cut(s) 65, 178, 320, 472
BstNI CCWGG 1 cut(s) 702
BstNSI RCATGY 1 cut(s) 488
BstSCI CCNGG 2 cut(s) 224, 700
BstUI CGCG 2 cut(s) 278, 323
BstV1I GCAGC 3 cut(s) 80, 250, 459
BstX2I RGATCY 2 cut(s) 367, 523
BstYI RGATCY 2 cut(s) 367, 523
BstZ17I GTATAC 1 cut(s) 445
BsuRI GGCC 3 cut(s) 281, 345, 858
Cac8I GCNNGC 3 cut(s) 146, 323, 591
CfoI GCGC 2 cut(s) 323, 325
Cfr10I RCCGGY 1 cut(s) 241
Cfr13I GGNCC 1 cut(s) 343
CseI GACGC 1 cut(s) 284
Csp6I GTAC 4 cut(s) 101, 262, 362, 738
CviAII CATG 3 cut(s) 460, 485, 533
CviQI GTAC 4 cut(s) 101, 262, 362, 738
DpnI GATC 4 cut(s) 369, 380, 525, 567
DpnII GATC 4 cut(s) 367, 378, 523, 565
Eam1104I CTCTTC 2 cut(s) 324, 627
EarI CTCTTC 2 cut(s) 324, 627
Ecl136II GAGCTC 1 cut(s) 222
Eco147I AGGCCT 1 cut(s) 858
Eco24I GRGCYC 1 cut(s) 224
Eco53kI GAGCTC 1 cut(s) 222
EcoICRI GAGCTC 1 cut(s) 222
EcoNI CCTNNNNNAGG 1 cut(s) 286
EcoRII CCWGG 1 cut(s) 700
EcoT22I ATGCAT 1 cut(s) 834
EcoT38I GRGCYC 1 cut(s) 224
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 3 cut(s) 463, 488, 536
FatI CATG 3 cut(s) 459, 484, 532
FauI CCCGC 1 cut(s) 137
FblI GTMKAC 2 cut(s) 273, 444
Fnu4HI GCNGC 6 cut(s) 69, 170, 239, 279, 473, 548
FriOI GRGCYC 1 cut(s) 224
Fsp4HI GCNGC 6 cut(s) 69, 170, 239, 279, 473, 548
GlaI GCGC 2 cut(s) 322, 324
GluI GCNGC 6 cut(s) 69, 170, 239, 279, 473, 548
GsaI CCCAGC 1 cut(s) 23
GsuI CTGGAG 1 cut(s) 732
HaeIII GGCC 3 cut(s) 281, 345, 858
HapII CCGG 4 cut(s) 201, 225, 242, 326
HgaI GACGC 1 cut(s) 284
HhaI GCGC 2 cut(s) 323, 325
Hin1I GRCGYC 2 cut(s) 5, 270
Hin1II CATG 3 cut(s) 463, 488, 536
Hin6I GCGC 2 cut(s) 321, 323
HinP1I GCGC 2 cut(s) 321, 323
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 437
HinfI GANTC 2 cut(s) 10, 116
HpaII CCGG 4 cut(s) 201, 225, 242, 326
HphI GGTGA 3 cut(s) 190, 328, 812
Hpy166II GTNNAC 2 cut(s) 274, 445
Hpy188I TCNGA 4 cut(s) 213, 426, 715, 760
Hpy188III TCNNGA 3 cut(s) 46, 371, 749
Hpy8I GTNNAC 2 cut(s) 274, 445
Hpy99I CGWCG 6 cut(s) 10, 108, 157, 272, 275, 278
HpyAV CCTTC 4 cut(s) 43, 82, 97, 664
HpyCH4III ACNGT 4 cut(s) 37, 413, 655, 742
HpyCH4IV ACGT 3 cut(s) 54, 103, 270
HpyCH4V TGCA 5 cut(s) 488, 539, 593, 644, 832
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 178, 320, 472
HpySE526I ACGT 3 cut(s) 54, 103, 270
Hsp92I GRCGYC 2 cut(s) 5, 270
Hsp92II CATG 3 cut(s) 463, 488, 536
HspAI GCGC 2 cut(s) 321, 323
Kzo9I GATC 4 cut(s) 367, 378, 523, 565
LmnI GCTCC 2 cut(s) 227, 305
Lsp1109I GCAGC 3 cut(s) 80, 250, 459
LweI GCATC 1 cut(s) 715
MaeII ACGT 3 cut(s) 54, 103, 270
MaeIII GTNAC 1 cut(s) 742
MalI GATC 4 cut(s) 369, 380, 525, 567
MbiI CCGCTC 1 cut(s) 308
MboI GATC 4 cut(s) 367, 378, 523, 565
MboII GAAGA 4 cut(s) 341, 614, 695, 816
MflI RGATCY 2 cut(s) 367, 523
MhlI GDGCHC 1 cut(s) 224
MluCI AATT 1 cut(s) 709
MlyI GAGTC 1 cut(s) 125
MmeI TCCRAC 1 cut(s) 411
MnlI CCTC 8 cut(s) 206, 228, 241, 608, 630, 766, 791, 830
Mph1103I ATGCAT 1 cut(s) 834
MseI TTAA 2 cut(s) 693, 842
MslI CAYNNNNRTG 2 cut(s) 38, 600
MspI CCGG 4 cut(s) 201, 225, 242, 326
MspR9I CCNGG 2 cut(s) 226, 702
MvaI CCWGG 1 cut(s) 702
MvnI CGCG 2 cut(s) 278, 323
MwoI GCNNNNNNNGC 4 cut(s) 65, 178, 320, 472
NciI CCSGG 1 cut(s) 226
NdeII GATC 4 cut(s) 367, 378, 523, 565
NlaIII CATG 3 cut(s) 463, 488, 536
NlaIV GGNNCC 2 cut(s) 344, 410
NmeAIII GCCGAG 1 cut(s) 243
NsiI ATGCAT 1 cut(s) 834
NspI RCATGY 1 cut(s) 488
OliI CACNNNNGTG 1 cut(s) 38
PauI GCGCGC 1 cut(s) 321
PceI AGGCCT 1 cut(s) 858
PcsI WCGNNNNNNNCGW 3 cut(s) 51, 96, 189
PfeI GAWTC 1 cut(s) 10
Pfl23II CGTACG 1 cut(s) 100
PflMI CCANNNNNTGG 1 cut(s) 352
PfoI TCCNGGA 1 cut(s) 224
PkrI GCNGC 6 cut(s) 70, 171, 240, 280, 474, 549
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
Psp124BI GAGCTC 1 cut(s) 224
Psp6I CCWGG 1 cut(s) 700
PspFI CCCAGC 1 cut(s) 19
PspGI CCWGG 1 cut(s) 700
PspLI CGTACG 1 cut(s) 100
PspN4I GGNNCC 2 cut(s) 344, 410
PspPI GGNCC 1 cut(s) 343
PsuI RGATCY 2 cut(s) 367, 523
PteI GCGCGC 1 cut(s) 321
RsaI GTAC 4 cut(s) 102, 263, 363, 739
RsaNI GTAC 4 cut(s) 101, 262, 362, 738
RseI CAYNNNNRTG 2 cut(s) 38, 600
SacI GAGCTC 1 cut(s) 224
SalI GTCGAC 1 cut(s) 272
SaqAI TTAA 2 cut(s) 693, 842
SatI GCNGC 6 cut(s) 69, 170, 239, 279, 473, 548
Sau3AI GATC 4 cut(s) 367, 378, 523, 565
Sau96I GGNCC 1 cut(s) 343
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 2 cut(s) 226, 702
SduI GDGCHC 1 cut(s) 224
SfaNI GCATC 1 cut(s) 715
SgrDI CGTCGACG 1 cut(s) 272
SmiMI CAYNNNNRTG 2 cut(s) 38, 600
Sse9I AATT 1 cut(s) 709
SseBI AGGCCT 1 cut(s) 858
SsiI CCGC 8 cut(s) 66, 144, 170, 278, 293, 308, 314, 548
SstI GAGCTC 1 cut(s) 224
StuI AGGCCT 1 cut(s) 858
StyD4I CCNGG 2 cut(s) 224, 700
TaaI ACNGT 4 cut(s) 37, 413, 655, 742
TaiI ACGT 3 cut(s) 57, 106, 273
TaqI TCGA 3 cut(s) 8, 273, 600
TasI AATT 1 cut(s) 709
TauI GCSGC 3 cut(s) 172, 281, 550
TfiI GAWTC 1 cut(s) 10
Tru1I TTAA 2 cut(s) 693, 842
Tru9I TTAA 2 cut(s) 693, 842
TseI GCWGC 3 cut(s) 68, 238, 472
TspDTI ATGAA 8 cut(s) 476, 509, 521, 522, 563, 621, 696, 825
TspGWI ACGGA 2 cut(s) 203, 318
Van91I CCANNNNNTGG 1 cut(s) 352
XagI CCTNNNNNAGG 1 cut(s) 286
XceI RCATGY 1 cut(s) 488
XcmI CCANNNNNNNNNTGG 1 cut(s) 576
XmiI GTMKAC 2 cut(s) 273, 444
ZraI GACGTC 1 cut(s) 271
Zsp2I ATGCAT 1 cut(s) 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.