Rmu_ssc0000309.1_g000017

Glyoxalase-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000309.1
OG0013486
Physical Location & Seq
Reverse (-)
50636 .. 54051
3416 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000309.1_g000017.1.cds

Sequence Viewer

Length: 909 bp
atggcgtcgaatctaaacccagcatttgcctacacggtggtgtacgtgaaggacgtagccaaatccgcagccttctattccaaagccttcggctacgccgtacgtcgtatagacgagtctcacagatggggagagcttgaaagcgggcagacgacgatagctttcacgccgcaaaaccagcacgagacggacgacctcaccggaaaagttcagaagccgagctccgggagtgagaggcagccggttgaggtttgctttgcgtaccccgacgtcgacgccgcctataaaagggcggtggagaacggagcggtggcggtgagcgcgccggaagagaaagagtggggccagaaagtggggtatgtacgagatcccgatgggatcgtgttgaataatagaaagaagcaaaggaaccgtttggcatatcagaagttggaaaagcttgtatactgctactacaacatgaagctgcagctgcgagatagacaggcaatgatgcatgtggtcaatgaaaacaactatatagatctacttcatgttgcaagtgagccgcttgataatggcattgatccacttcattattggattatgcctgcacacctcgatgaggaagctggaaaacctcttccacaagttgtagaaactgcaacaaatactggtggtacagtaactccagatagttctgatgatggtggagagggtggaagtggaacaggtggtggaggtgaaagatatgaatatagacaatcttctatggatagagaattaaacccatttaccggcctgaatgacatgcagacacaccgccagacaggttacaacctcagcatcggaccaccgcccgatcgccaccgacgcgccgccacgcgccgcgccaaaatagcatcagaagcttctgaagctgggaactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase Glyoxalase_2

Protein Analysis

302

Amino Acids

33.53

Weight (kDa)

5.8

Isoelectric Point (pI)

36.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013486)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07645
fragaria_vesca FvH4_3g35580 FvH4_3g35610
malus_domestica MD07G1053700.v1.1 MD11G1144900.v1.1 MD14G1025700.v1.1
prunus_persica Prupe.6G080800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0063801
rosa_laevigata RLG00000035673
rosa_multiflora Rmu_sc0005685.1_g000007 Rmu_ssc0000309.1_g000017
rosa_roxburghii Rroxscaffold_1G00017030
rosa_rugosa Rorug05G0360000 Rorug05G0360000
rosa_samantha Rh5BG434400 Rh5CG457600 Rh5DG447500
rosa_wichuraiana Rw5G039320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 273
AccB7I CCANNNNNTGG 1 cut(s) 352
AccBSI CCGCTC 1 cut(s) 308
AccI GTMKAC 2 cut(s) 273, 444
AccII CGCG 4 cut(s) 323, 855, 865, 870
AclWI GGATC 3 cut(s) 362, 386, 560
AcyI GRCGYC 3 cut(s) 5, 270, 276
AfaI GTAC 5 cut(s) 44, 102, 263, 363, 661
AfiI CCNNNNNNNGG 5 cut(s) 224, 288, 352, 604, 776
AgsI TTSAA 2 cut(s) 140, 388
AleI CACNNNNGTG 1 cut(s) 38
AluBI AGCT 9 cut(s) 136, 161, 222, 439, 466, 472, 611, 890, 899
AluI AGCT 9 cut(s) 136, 161, 222, 439, 466, 472, 611, 890, 899
Alw21I GWGCWC 1 cut(s) 224
Alw26I GTCTC 2 cut(s) 123, 179
AlwI GGATC 3 cut(s) 362, 386, 560
AoxI GGCC 2 cut(s) 343, 778
ApeKI GCWGC 5 cut(s) 68, 238, 466, 469, 472
AspLEI GCGC 5 cut(s) 323, 325, 857, 867, 872
AspS9I GGNCC 2 cut(s) 343, 830
AsuC2I CCSGG 1 cut(s) 226
AsuHPI GGTGA 3 cut(s) 190, 328, 734
AvaII GGWCC 1 cut(s) 830
BanII GRGCYC 1 cut(s) 224
BauI CACGAG 1 cut(s) 182
Bbv12I GWGCWC 1 cut(s) 224
BbvCI CCTCAGC 1 cut(s) 821
BbvI GCAGC 5 cut(s) 80, 250, 453, 459, 481
BccI CCATC 3 cut(s) 120, 368, 680
BceAI ACGGC 1 cut(s) 83
BcnI CCSGG 1 cut(s) 226
BcoDI GTCTC 2 cut(s) 123, 179
BfmI CTRYAG 1 cut(s) 467
BglII AGATCT 1 cut(s) 523
Bme1390I CCNGG 1 cut(s) 226
Bme18I GGWCC 1 cut(s) 830
BmgT120I GGNCC 2 cut(s) 343, 830
BmiI GGNNCC 2 cut(s) 344, 410
BmrFI CCNGG 1 cut(s) 226
BmsI GCATC 3 cut(s) 483, 834, 890
BpmI CTGGAG 1 cut(s) 654
Bpu10I CCTNAGC 1 cut(s) 821
BpuMI CCSGG 1 cut(s) 226
BsaAI YACGTR 1 cut(s) 46
BsaHI GRCGYC 3 cut(s) 5, 270, 276
BsaWI WCCGGW 1 cut(s) 200
BsaXI ACNNNNNCTCC 2 cut(s) 652, 682
Bsc4I CCNNNNNNNGG 5 cut(s) 224, 288, 352, 604, 776
Bse118I RCCGGY 2 cut(s) 241, 776
Bse1I ACTGG 1 cut(s) 658
Bse3DI GCAATG 1 cut(s) 495
BseLI CCNNNNNNNGG 5 cut(s) 224, 288, 352, 604, 776
BseMI GCAATG 1 cut(s) 495
BseMII CTCAG 1 cut(s) 835
BseNI ACTGG 1 cut(s) 658
BsePI GCGCGC 1 cut(s) 321
BseXI GCAGC 5 cut(s) 80, 250, 453, 459, 481
BseYI CCCAGC 2 cut(s) 19, 899
BsgI GTGCAG 1 cut(s) 576
Bsh1236I CGCG 4 cut(s) 323, 855, 865, 870
Bsh1285I CGRYCG 1 cut(s) 844
BshFI GGCC 2 cut(s) 345, 780
BsiEI CGRYCG 1 cut(s) 844
BsiHKAI GWGCWC 1 cut(s) 224
BsiSI CCGG 5 cut(s) 201, 225, 242, 326, 777
BsiWI CGTACG 1 cut(s) 100
BslI CCNNNNNNNGG 5 cut(s) 224, 288, 352, 604, 776
BsmAI GTCTC 2 cut(s) 123, 179
BsmBI CGTCTC 1 cut(s) 179
BsnI GGCC 2 cut(s) 345, 780
Bsp1286I GDGCHC 1 cut(s) 224
Bsp143I GATC 5 cut(s) 367, 378, 523, 565, 841
BspANI GGCC 2 cut(s) 345, 780
BspCNI CTCAG 1 cut(s) 834
BspFNI CGCG 4 cut(s) 323, 855, 865, 870
BspLI GGNNCC 2 cut(s) 344, 410
BspMAI CTGCAG 1 cut(s) 471
BspPI GGATC 3 cut(s) 362, 386, 560
BsrBI CCGCTC 1 cut(s) 308
BsrDI GCAATG 1 cut(s) 495
BsrFI RCCGGY 2 cut(s) 241, 776
BsrI ACTGG 1 cut(s) 658
BssAI RCCGGY 2 cut(s) 241, 776
BssHII GCGCGC 1 cut(s) 321
BssMI GATC 5 cut(s) 367, 378, 523, 565, 841
BssNAI GTATAC 1 cut(s) 445
BssNI GRCGYC 3 cut(s) 5, 270, 276
BssSI CACGAG 1 cut(s) 182
Bst1107I GTATAC 1 cut(s) 445
Bst2BI CACGAG 1 cut(s) 182
Bst4CI ACNGT 3 cut(s) 37, 413, 664
Bst6I CTCTTC 2 cut(s) 324, 627
BstACI GRCGYC 3 cut(s) 5, 270, 276
BstBAI YACGTR 1 cut(s) 46
BstC8I GCNNGC 3 cut(s) 146, 323, 591
BstDEI CTNAG 1 cut(s) 821
BstENI CCTNNNNNAGG 2 cut(s) 286, 602
BstFNI CGCG 4 cut(s) 323, 855, 865, 870
BstHHI GCGC 5 cut(s) 323, 325, 857, 867, 872
BstKTI GATC 5 cut(s) 370, 381, 526, 568, 844
BstMAI GTCTC 2 cut(s) 123, 179
BstMBI GATC 5 cut(s) 367, 378, 523, 565, 841
BstMCI CGRYCG 1 cut(s) 844
BstMWI GCNNNNNNNGC 8 cut(s) 65, 178, 320, 472, 852, 878, 887, 896
BstNSI RCATGY 2 cut(s) 500, 793
BstSCI CCNGG 1 cut(s) 224
BstSFI CTRYAG 1 cut(s) 467
BstUI CGCG 4 cut(s) 323, 855, 865, 870
BstV1I GCAGC 5 cut(s) 80, 250, 453, 459, 481
BstX2I RGATCY 2 cut(s) 367, 523
BstYI RGATCY 2 cut(s) 367, 523
BstZ17I GTATAC 1 cut(s) 445
BsuRI GGCC 2 cut(s) 345, 780
Cac8I GCNNGC 3 cut(s) 146, 323, 591
CfoI GCGC 5 cut(s) 323, 325, 857, 867, 872
Cfr10I RCCGGY 2 cut(s) 241, 776
Cfr13I GGNCC 2 cut(s) 343, 830
CseI GACGC 2 cut(s) 284, 861
Csp6I GTAC 5 cut(s) 43, 101, 262, 362, 660
CviAII CATG 4 cut(s) 460, 497, 533, 790
CviQI GTAC 5 cut(s) 43, 101, 262, 362, 660
DdeI CTNAG 1 cut(s) 821
DpnI GATC 5 cut(s) 369, 380, 525, 567, 843
DpnII GATC 5 cut(s) 367, 378, 523, 565, 841
Eam1104I CTCTTC 2 cut(s) 324, 627
EarI CTCTTC 2 cut(s) 324, 627
Ecl136II GAGCTC 1 cut(s) 222
Eco24I GRGCYC 1 cut(s) 224
Eco47I GGWCC 1 cut(s) 830
Eco53kI GAGCTC 1 cut(s) 222
EcoICRI GAGCTC 1 cut(s) 222
EcoNI CCTNNNNNAGG 2 cut(s) 286, 602
EcoT22I ATGCAT 1 cut(s) 498
EcoT38I GRGCYC 1 cut(s) 224
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 4 cut(s) 463, 500, 536, 793
FatI CATG 4 cut(s) 459, 496, 532, 789
FauI CCCGC 1 cut(s) 137
FblI GTMKAC 2 cut(s) 273, 444
FriOI GRGCYC 1 cut(s) 224
GlaI GCGC 5 cut(s) 322, 324, 856, 866, 871
GsaI CCCAGC 2 cut(s) 23, 903
GsuI CTGGAG 1 cut(s) 654
HaeIII GGCC 2 cut(s) 345, 780
HapII CCGG 5 cut(s) 201, 225, 242, 326, 777
HgaI GACGC 2 cut(s) 284, 861
HhaI GCGC 5 cut(s) 323, 325, 857, 867, 872
Hin1I GRCGYC 3 cut(s) 5, 270, 276
Hin1II CATG 4 cut(s) 463, 500, 536, 793
Hin6I GCGC 5 cut(s) 321, 323, 855, 865, 870
HinP1I GCGC 5 cut(s) 321, 323, 855, 865, 870
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 2 cut(s) 437, 888
HinfI GANTC 2 cut(s) 10, 116
HpaII CCGG 5 cut(s) 201, 225, 242, 326, 777
HphI GGTGA 3 cut(s) 190, 328, 734
Hpy166II GTNNAC 3 cut(s) 43, 274, 445
Hpy188I TCNGA 6 cut(s) 213, 426, 682, 830, 886, 895
Hpy188III TCNNGA 2 cut(s) 371, 671
Hpy8I GTNNAC 3 cut(s) 43, 274, 445
Hpy99I CGWCG 7 cut(s) 10, 108, 157, 272, 275, 278, 855
HpyAV CCTTC 3 cut(s) 43, 82, 97
HpyCH4III ACNGT 3 cut(s) 37, 413, 664
HpyCH4IV ACGT 4 cut(s) 45, 54, 103, 270
HpyCH4V TGCA 6 cut(s) 469, 496, 539, 593, 644, 793
HpyF10VI GCNNNNNNNGC 8 cut(s) 65, 178, 320, 472, 852, 878, 887, 896
HpyF3I CTNAG 1 cut(s) 821
HpySE526I ACGT 4 cut(s) 45, 54, 103, 270
Hsp92I GRCGYC 3 cut(s) 5, 270, 276
Hsp92II CATG 4 cut(s) 463, 500, 536, 793
HspAI GCGC 5 cut(s) 321, 323, 855, 865, 870
Kzo9I GATC 5 cut(s) 367, 378, 523, 565, 841
LmnI GCTCC 2 cut(s) 227, 305
Lsp1109I GCAGC 5 cut(s) 80, 250, 453, 459, 481
LweI GCATC 3 cut(s) 483, 834, 890
MaeII ACGT 4 cut(s) 45, 54, 103, 270
MaeIII GTNAC 2 cut(s) 664, 812
MalI GATC 5 cut(s) 369, 380, 525, 567, 843
MbiI CCGCTC 1 cut(s) 308
MboI GATC 5 cut(s) 367, 378, 523, 565, 841
MboII GAAGA 3 cut(s) 341, 614, 738
MflI RGATCY 2 cut(s) 367, 523
MhlI GDGCHC 1 cut(s) 224
MluCI AATT 1 cut(s) 761
MlyI GAGTC 1 cut(s) 125
MmeI TCCRAC 1 cut(s) 411
MnlI CCTC 9 cut(s) 206, 228, 241, 598, 608, 630, 688, 713, 830
Mph1103I ATGCAT 1 cut(s) 498
MseI TTAA 1 cut(s) 764
MslI CAYNNNNRTG 2 cut(s) 38, 600
MspA1I CMGCKG 1 cut(s) 472
MspI CCGG 5 cut(s) 201, 225, 242, 326, 777
MspR9I CCNGG 1 cut(s) 226
MteI GCGCNGCGC 1 cut(s) 868
MvnI CGCG 4 cut(s) 323, 855, 865, 870
MwoI GCNNNNNNNGC 8 cut(s) 65, 178, 320, 472, 852, 878, 887, 896
NciI CCSGG 1 cut(s) 226
NdeII GATC 5 cut(s) 367, 378, 523, 565, 841
NlaIII CATG 4 cut(s) 463, 500, 536, 793
NlaIV GGNNCC 2 cut(s) 344, 410
NmeAIII GCCGAG 1 cut(s) 243
NsiI ATGCAT 1 cut(s) 498
NspI RCATGY 2 cut(s) 500, 793
OliI CACNNNNGTG 1 cut(s) 38
PauI GCGCGC 1 cut(s) 321
PcsI WCGNNNNNNNCGW 3 cut(s) 51, 96, 189
PfeI GAWTC 1 cut(s) 10
Pfl23II CGTACG 1 cut(s) 100
PflMI CCANNNNNTGG 1 cut(s) 352
PfoI TCCNGGA 1 cut(s) 224
Ple19I CGATCG 1 cut(s) 844
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
Ppu21I YACGTR 1 cut(s) 46
Psp124BI GAGCTC 1 cut(s) 224
PspFI CCCAGC 2 cut(s) 19, 899
PspLI CGTACG 1 cut(s) 100
PspN4I GGNNCC 2 cut(s) 344, 410
PspPI GGNCC 2 cut(s) 343, 830
PstI CTGCAG 1 cut(s) 471
PsuI RGATCY 2 cut(s) 367, 523
PteI GCGCGC 1 cut(s) 321
PvuI CGATCG 1 cut(s) 844
PvuII CAGCTG 1 cut(s) 472
RsaI GTAC 5 cut(s) 44, 102, 263, 363, 661
RsaNI GTAC 5 cut(s) 43, 101, 262, 362, 660
RseI CAYNNNNRTG 2 cut(s) 38, 600
SacI GAGCTC 1 cut(s) 224
SalI GTCGAC 1 cut(s) 272
SaqAI TTAA 1 cut(s) 764
Sau3AI GATC 5 cut(s) 367, 378, 523, 565, 841
Sau96I GGNCC 2 cut(s) 343, 830
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 1 cut(s) 226
SduI GDGCHC 1 cut(s) 224
SfaNI GCATC 3 cut(s) 483, 834, 890
SfcI CTRYAG 1 cut(s) 467
SgrDI CGTCGACG 1 cut(s) 272
SinI GGWCC 1 cut(s) 830
SmiMI CAYNNNNRTG 2 cut(s) 38, 600
Sse9I AATT 1 cut(s) 761
SstI GAGCTC 1 cut(s) 224
StyD4I CCNGG 1 cut(s) 224
TaaI ACNGT 3 cut(s) 37, 413, 664
TaiI ACGT 4 cut(s) 48, 57, 106, 273
TaqI TCGA 3 cut(s) 8, 273, 600
TasI AATT 1 cut(s) 761
TauI GCSGC 5 cut(s) 172, 281, 550, 860, 870
TfiI GAWTC 1 cut(s) 10
Tru1I TTAA 1 cut(s) 764
Tru9I TTAA 1 cut(s) 764
TseI GCWGC 5 cut(s) 68, 238, 466, 469, 472
TspDTI ATGAA 5 cut(s) 476, 521, 522, 563, 747
TspGWI ACGGA 2 cut(s) 203, 318
Van91I CCANNNNNTGG 1 cut(s) 352
VpaK11BI GGWCC 1 cut(s) 830
XagI CCTNNNNNAGG 2 cut(s) 286, 602
XceI RCATGY 2 cut(s) 500, 793
XcmI CCANNNNNNNNNTGG 1 cut(s) 576
XmiI GTMKAC 2 cut(s) 273, 444
ZraI GACGTC 1 cut(s) 271
Zsp2I ATGCAT 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.