RchiOBHm_Chr5g0071961

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0011193
Physical Location & Seq
Reverse (-)
77760996 .. 77772192
11197 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5715 bp
ATGGGGAAGCAGAAGGGAGACGGAGCTAGGAGCAAGGCCCGCCCTTCAAGTAGCAGTTTGGCGGCGTCGCTGTTGCCGTCGGGTTCTGCTGCGGCGGTGGGCTTCGGTGGCTACGTCGGAGGCTCTCGCCTCGATGCTCCTCCTTCCGGCGATGATTCAAGGCCCTTTTTGGAAGTAGATAGTGATTTGGCTCTACATTTGAAGAGGCTTGCAAGGAAAGATCCCACCACCAAGCTTAAAGCTTTGGCATCTCTATCCACACTGTTGAAGGAAAAGTCCACGAAGGACATAGTAGCGGCCATTCCACAATGGGGATTTGAATACAAAAGGCTTGTGGTGGACTACAATAGGGATGTGCGGCGAGCAACTCATGACACAATGACCATTCTTGTCACCGCCGTTGGGAGAGATTTAGCGCCACAGCTGAAATCTTTGATGGGACCGTGGTGGTTTTCTCAATTTGACCCAGTCTCTGAAGTTTCTCAAGCTGCAAAGCGATCATTTCAGGCAGTCTTTTCAGCTCCAGAAAAGAGACTAGATGCTTTAATTTTATGCACGGCAGAGGTTTTTATTTATTTGGAAGAAAATCTGAGGCTTACACCGCAAAATATGTCCGATAAAGGAACTGCTCTGGATGAATTGCAAGAGATGCATCAGCAGGTGATATCTTCATCATTACTGGCATTGGCCACACTTCTTGATGTCTTGGTTTGCTTACAACTAGAAAGACCAGGCACCGAAAACCTAGCTGCTCAACCAAAACATGCTTTGAAGGCTAGGGAAACTGCTATTTCTTGTGCCGACAAGTTGTTCACTGCTCATAAATATTTCTTAGACTTCTTGAAATCCCCAAGTCCTGCTATCCGTTCAGCCACATATTCTGTATTGAGTAGTTTTATAAAAAACGTCCCTCAAGCCTTTAATGAAGGAAATATGAAAACTCTTGCTGCTGCACTATTAGGTGGATTTCAGGAGAAGGATCCTGCTTGTCATTCATCAATGTGGGATGCAATTTTACTGTTTTCTAATAAATTTCCTGAAAGTTGGACTTCTGTCAATGTACAGAAAACTGTACTGAATCGGTTTTGGGATTTCCTTAGAAATAGGTGCTTTGGATCTCAACAGGTTTCTTATCCATCTTTGGTTCTATTCTTACACACCGTGCCATCTAAAGCAGTCGTTGCAGAAACTTTTTTTCTTGAATTCTTCAAGAACCTCTGGGCAGGAAGGAATCCATCGCATTCCTTAAATGCAGATAGAGTGGCATTTTTTCGAGCATTACAAGAATGCTTTCTTTGGGCATTGCACAATGTCTCAAGATATTGCAATGGAGTGGATTCCATCAGTGATTTTCAAGCAACTCTTGTTAAAAATGTTCTGGTAAAGCTTTTGTGGCAAGACTACATTTTCTCTAGTAGCTCAAGAAAGGAGGAGAAAACGTCCCCCGGATTATCAGTAGATTCATGTGAAAGTGATTTAGCTTCCAACAAGAAGACAGTTGAAACATTGAATATCACGTACCCAATGAGCTACTTTAGAGAGTTGGCAAATTGCATTGTTGGGGTTCTTTCAGGCATTGATTTGCTGGAGCATGATTTGCTCTCAGCTTTTGCTGCAGAATTTCAGGAGAATTGTCAGAGTTTCTTTCAGCATGCTAGTAACTTGGAAAGAGAAAGTGAGTTTGCGGAGCGAGTTATACAGTTTATATCATTACTTGGAGAACATGCAATGCAGAATGGTCGAGGTTGGCCTTTGGCTTCTCTAGTTGGACCAATGTTGGTTAATTCCTTTGCATTGATGAGATCACATGATTCACCAAGTTGTGTGAAGATTCTAGCAGTGGCTGTTTCTGTATTTGGACCTCACAAGATAGTCCACGAACTTCTTATTCACAATACGTCTCCACATAGTTATTCTCCTGATGAGGGTGATAGAGCATTAGAGGAAGATAGATTCCTGCAAATGTTCAATGGAACTTTTGTTCCATGGTGTCTTAGTGGGAATAGTTACTCTTTAAGTGCTCGTCTTGACCTGTTACTTGCATTGCTTGATGATGAATATTTCTCTGAGCAGTGGGATAGTGTTATCAGATATGCCACTAACCTGGAGCATTCTGGATCTGCACCCTGCTCTTTAGATTCTGACCGGATAACTATCTTAGCAATGCTTTTACAGAAAGTAAGAAACGGAATCACAAACACAAAGGTAGGAGTTTCCATCAGCACAAAAATGGGCAACCCAGATCATTGGCATCATGAGCTTCTAGAATCAACTGCTGTTGCTGTTGCTCGATCCTCTCCTCCTCTCGGAGCTTCTAATTCTCAATTCTTGTGTACTGTTGTTGCTGGTTCAACAAAGATCAATCAAATTTCTCTGGTGTCAAGAAATACATTGATCCTGATATTTGAAGAGGTTTTCAAGAAGTTACTTTCTTTTATTATGGCATCCTCTTTTACTTGGGTTAGGGATGCAGGTTCTCTATTGACTGCCGGGGCAAACACTATTGGGCCAGAATTTGAAAATTCTGTGAGCATGTTTGAGATGGCTCAATTTGCTCTTGAAGTACTTGATGGCGGCTTATATTTATTAAAGACACTTGGTGAAGAAAGTGGGTTGAGTTCAGTTATTTTAGCTGCAATTTTTCTGATTGACTGGGAGTTTCTGGTATTACTGACAACGATAGATGATGCACCTGATGATAAATCAAGGGAAAAATTGAAGGCCAGGCTGGGATTTGGTGAATCTTTTCATGCATTTAGATGTAAGATAAGTAATCAATTCTGGAAAACACTCAGCTTACACAATCGAAAGGCACTTGGAAAAATTCTGATTCAGTGTATGAGATCCGCCATCTTCAATGAAGAGGAATTAGACACAGAGAAGTTCACATCTCTGTGCTGCTTGTGGATGCTTGAAATTTTGGACTGTCTCTCTCAGGATCCCTATGAGGAACAAAATCTGCTGGACCAACTTCTCTGCCAAGGTGATAGATGGCCTTTGTGGATAGTTCCGGATTTTAGCAGGCCAGAGGGAATAGTTGCCAAGGACTTCTCTATCCAATTGGACTTTGGCCACCGCAAGTTTATTTCATTTATTGACAAGATGATATCAGAAATTGGGATTCATAGAGTTGTTGGGGGTTATGCTAGATATACTCTCCCTCCCTCTGTGGAAGCAACATATGAAAAGCCGACTCGATCCTGGCTTGCTGCTGAAATATTATGCTCGTGGAAATGGCCAGGAGGAAGTGCTGTAGCTTCATTCTTACCTTCATTAAGTGCCTATGCAAAGAGTAAAAATTTCTCTTCCCAGGAGAGCTTGGTAGATTTTATTTTCAACATTCTACTTGATGGCACACTTGTTCAGGGAGGATGTGCTGCACAGAATTTTGTCTATCTGTGTCCTACTTCAAGTGATGAAGTGGAGGATATTGAAGAACCATTCTTAAGAGCTCTTGTAGCTTTTCTTTTAACTTTGTTTAATGATAAAATATGGGGTACTGAGAAAGCTATGGAGCTGTTTGCATTGCTTGTAAATAAACTTTATGTTGGAGAAGCAACAAATGCAAACTGTTTGAGGATTCTTCCTCTGATTGTTAATGTTCTTATTCAACCATTAAGTCAAAGAAGCATCAGATGTAATGACTCTAGTGGAGACGCTCAACATGATTCTTCTGGGGAAAATCATGTGCAAGTTGTTATTGAAGGCTGGCTAAAAAAAGCTCTATCATTTCCACCTTTGATCATGTGGTGGCAAACAGGAGAAGATATGGAAGATTGGATGCAGCTGGTTATTTCTTGTTATCCCATTAGTGCAGTAGAGGACATACAAACACCGAAGCTGGAGAGAGAGATCAGCTCGGTAGAGAGAAAGCTCCTACTTGAATTATTCCGGAAGCAGAGACATGGTGTTGGCGCACCAGCTGTAATTAATCAGCTTCCAGTTGTGCAGATGTTGCTATCAAAGCTTATGGTCGTTTCAGTTGGTTATTGTTGGAAAGAATTTGATGAAGAAGATTGGGAGTTTGTGTTATCTCAGATAAGACGCTGGCTGCAGTCTATTGTTGTCATGATGGAGGAAATTGCTGAAAATGTCAACGACACTATCACTAGCAGCTTTACTTCTGATAACTTGGATGATGTCGTTGATAACCTTGGCAAAATTGTTTTCGTTTCAGATCCCTTTCCTATAGACATTGCCAAAAATGCCCTTTTATCATTTTCTCTGTCCTGTGGACCTTTTGGTCGCCAACAAGCAGAAGATGCAGATAACTTAAATCCCGTGAGAACAGAAAGATGGGACCCCCTCAAAAATAGAATTTTAGAAGGCATCCTTCGATTGTTGTTCTGCACGGGTATTGCTGAGGCCATTGCAAGCTCCTGTTGTCACGAGGCTGCATTTATTGTATCAGCGGCGCGATTTGAACATTCCTATTTCTGGGAATTGGTGGCCTTAAATGTGGTCAACTCATCAACAGATGCCATAGATAGAGCAGTGAAGTCAGTTGAATTCTGGGGGCTGAGCAAAGGACCCATTAGCTCTTTGTATGCAATCCTTTTTTCTGCCAAAACAGTTCCTCTGTTGCAGTTCGCTGCTTACTTTATTCTTTCAACTGAACTTGTTTTACGCCTGGCTATTGTTGAAGAAGACAAAAGTTACTTGGATGGCGTTAGTAACAATGAAGAGGTTTCATCCTCTCTTGACATGTCGACAGAAACGGATATTCATTTGAGAGCTGAAATATCTTGCATGATTGAAAAGTTACCTAGTAATGTTCTCGAGATGGATTTAGTGGCAGATCAAAGGGTACATGTTTTTCTTGCGTGGTCCTTGTTGTTATCTCATTTAGGGTCATTGCCATCATCATCACCTGCAAGGGAGAGACTAGTCCAGTACGTACAAGATTCTGCTAATCCAGTAATATTAGATTGCCTCTTCCAGCATATTCCTCTAGAACTGTGGATTCTTAAGAAGAAAGACGAAGAGCTTCCTGCTGGCATAGCAGAAGCTGCAGCTGCAGCAACACGTTCCATTAGAACTGGTTCCTTATTGTTTTCGGTACAGTCTCTCTGGCCTGTTGAACCACTGAAAATGGCTTCGCTTGCTGGAGCAATGTTTGGTCTGATGCTCCACATTCTTCCCGCCTATGTAAGACAGTGGTCCAATGATTTACGTGACCGCTCTACATTATCAGGAATTGAATCGTTCACAAGAGCATGGTGCAGCCCACATCTCATCGCTGGCGAATTAAGTCAGATTAAAAAGGATGAAATTGCTGACGAGAATTTTACCATTGCTGTAAGCAAATCAGCAAATGAGGTTGTTGCTACTTATACAAAGGATGAGACTGCAATGAATCTAGTAATCCGTCTTCCTTCATCTTACCCATTACGGCCTGTTGATGTTGATTGTACAAGGAGTCTAGGTATTAGTGAAGCGAAGCAGAGGAAATGGTCAATGTCTATGACATTATTCGTACGGAATCAGAATGGAGCTTTAGCAGAAGCTATCCGGATATGGAAGCGCAACTTTGATAAGGAGTTTGAAGGTGTCGAGGAGTGTCCTATTTGTTATAGTGTCATCCATACCGTCAACCACGCCCTTCCACGTCTAGCGTGCAAGACCTGCAAGCACAAGTTCCATTCGGCCTGCCTCTATAAGTGGTTCTCAACTTCTCACAAATCAACTTGCCCATTGTGCCAGTCTCCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

1904

Amino Acids

212.54

Weight (kDa)

5.5

Isoelectric Point (pI)

46.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ltn1_1st PF22958 60 - 386 3.9e-90 E3 ubiquitin-protein ligase listerin, N-terminal domain
LTN1_E3_ligase_6th PF22999 1511 - 1754 4.8e-28 LTN1 family HEAT repeat region
UBC_like PF23009 1764 - 1847 1.1e-29 Ubiquitin conjugating domain-like
zf-RING_2 PF13639 1853 - 1901 7.6e-07 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58410 AT5G58410
fragaria_vesca FvH4_3g39950 FvH4_3g39950 FvH4_3g39950
malus_domestica MD03G1058100.v1.1
prunus_persica Prupe.6G045900_v2.0.a1 Prupe.6G045900_v2.0.a1 Prupe.6G045900_v2.0.a1
pyrus_communis pycom03g04640
rosa_chinensis RchiOBHm_Chr5g0071961
rosa_laevigata RLG00000036266
rosa_multiflora Rmu_sc0012806.1_g000004
rosa_roxburghii Rroxscaffold_1G00009240
rosa_rugosa Rorug05G0415700
rosa_samantha Rh5AG471700 Rh5BG491700 Rh5CG515500 Rh5DG503100
rosa_wichuraiana Rw5G043850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 899
AarI CACCTGC 2 cut(s) 649, 4879
AasI GACNNNNNNGTC 1 cut(s) 4242
Acc36I ACCTGC 4 cut(s) 649, 2462, 4879, 5633
AccB1I GGYRCC 1 cut(s) 734
AccBSI CCGCTC 1 cut(s) 5181
AccI GTMKAC 1 cut(s) 4709
AccII CGCG 1 cut(s) 4417
AccIII TCCGGA 3 cut(s) 3010, 3891, 5511
AcoI YGGCCR 4 cut(s) 297, 687, 3070, 3236
AcuI CTGAAG 1 cut(s) 495
AcyI GRCGYC 1 cut(s) 65
AdeI CACNNNGTG 2 cut(s) 1162, 2600
AfiI CCNNNNNNNGG 6 cut(s) 146, 311, 402, 1925, 2306, 4437
AflII CTTAAG 2 cut(s) 3445, 4967
AflIII ACRYGT 3 cut(s) 4704, 4810, 5024
AhlI ACTAGT 1 cut(s) 4885
AjiI CACGTC 1 cut(s) 5609
AjnI CCWGG 7 cut(s) 730, 2103, 2723, 3200, 3238, 3309, 4629
AloI GAACNNNNNNTCC 4 cut(s) 1965, 1966, 1997, 1998
Alw21I GWGCWC 2 cut(s) 2023, 3454
AlwNI CAGNNNCTG 3 cut(s) 473, 1844, 5009
Ama87I CYCGRG 1 cut(s) 4778
Aor13HI TCCGGA 3 cut(s) 3010, 3891, 5511
AseI ATTAAT 1 cut(s) 3930
Asp700I GAANNNNTTC 4 cut(s) 1290, 2745, 4986, 5041
AspLEI GCGC 4 cut(s) 418, 3917, 4417, 5526
AsuC2I CCSGG 2 cut(s) 1446, 2491
AsuHPI GGTGA 8 cut(s) 385, 673, 1806, 1940, 2612, 2750, 2996, 4860
AvaI CYCGRG 1 cut(s) 4778
AvaII GGWCC 9 cut(s) 440, 1769, 1859, 2965, 4235, 4300, 4529, 4827, 5160
BalI TGGCCA 3 cut(s) 689, 3072, 3238
BamHI GGATCC 2 cut(s) 979, 2938
BanI GGYRCC 1 cut(s) 734
BanII GRGCYC 1 cut(s) 3454
BarI GAAGNNNNNNTAC 2 cut(s) 5355, 5387
BauI CACGAG 2 cut(s) 3226, 4388
BbsI GAAGAC 3 cut(s) 1499, 4653, 5363
Bbv12I GWGCWC 2 cut(s) 2023, 3454
BbvCI CCTCAGC 1 cut(s) 4362
BceAI ACGGC 4 cut(s) 61, 383, 573, 5408
BcgI CGANNNNNNTGC 2 cut(s) 1721, 1755
BciT130I CCWGG 7 cut(s) 732, 2105, 2725, 3202, 3240, 3311, 4631
BclI TGATCA 1 cut(s) 3741
BcnI CCSGG 2 cut(s) 1446, 2491
BcuI ACTAGT 1 cut(s) 4885
BfmI CTRYAG 6 cut(s) 1614, 3252, 4052, 4188, 5010, 5016
BfoI RGCGCY 1 cut(s) 419
BfrI CTTAAG 2 cut(s) 3445, 4967
BfuAI ACCTGC 4 cut(s) 649, 2462, 4879, 5633
BlpI GCTNAGC 1 cut(s) 4520
BmcAI AGTACT 1 cut(s) 2565
Bme1390I CCNGG 9 cut(s) 732, 1446, 2105, 2491, 2725, 3202, 3240, 3311, 4631
Bme18I GGWCC 9 cut(s) 440, 1769, 1859, 2965, 4235, 4300, 4529, 4827, 5160
BmeT110I CYCGRG 1 cut(s) 4778
BmgBI CACGTC 1 cut(s) 5609
BmiI GGNNCC 8 cut(s) 441, 736, 981, 2940, 4301, 4302, 4531, 5044
BmrFI CCNGG 9 cut(s) 732, 1446, 2105, 2491, 2725, 3202, 3240, 3311, 4631
BmrI ACTGGG 2 cut(s) 461, 2662
BmuI ACTGGG 2 cut(s) 461, 2662
BoxI GACNNNNGTC 2 cut(s) 1052, 5158
BpiI GAAGAC 3 cut(s) 1499, 4653, 5363
BplI GAGNNNNNCTC 2 cut(s) 3842, 3874
BpmI CTGGAG 5 cut(s) 507, 1607, 2126, 3863, 5127
Bpu10I CCTNAGC 1 cut(s) 4362
Bpu1102I GCTNAGC 1 cut(s) 4520
BpuEI CTTGAG 4 cut(s) 468, 897, 1300, 1405
BpuMI CCSGG 2 cut(s) 1446, 2491
BsaAI YACGTR 3 cut(s) 1518, 4897, 5174
BsaBI GATNNNNATC 1 cut(s) 2153
BsaHI GRCGYC 1 cut(s) 65
BsaJI CCNNGG 8 cut(s) 443, 1444, 1985, 2490, 2980, 3042, 3309, 4153
BsaWI WCCGGW 4 cut(s) 2145, 3010, 3891, 5511
BsaXI ACNNNNNCTCC 8 cut(s) 1424, 1454, 3145, 3175, 4013, 4043, 5410, 5440
Bsc4I CCNNNNNNNGG 6 cut(s) 146, 311, 402, 1925, 2306, 4437
Bse1I ACTGG 8 cut(s) 467, 684, 2657, 3941, 4891, 4916, 5044, 5701
Bse8I GATNNNNATC 1 cut(s) 2153
BseAI TCCGGA 3 cut(s) 3010, 3891, 5511
BseBI CCWGG 7 cut(s) 732, 2105, 2725, 3202, 3240, 3311, 4631
BseDI CCNNGG 8 cut(s) 443, 1444, 1985, 2490, 2980, 3042, 3309, 4153
BseJI GATNNNNATC 1 cut(s) 2153
BseLI CCNNNNNNNGG 6 cut(s) 146, 311, 402, 1925, 2306, 4437
BseMII CTCAG 9 cut(s) 581, 1617, 2058, 2806, 2948, 3492, 4049, 4353, 4511
BseNI ACTGG 8 cut(s) 467, 684, 2657, 3941, 4891, 4916, 5044, 5701
BseRI GAGGAG 5 cut(s) 129, 1445, 2289, 2292, 5570
BseYI CCCAGC 1 cut(s) 2728
BsgI GTGCAG 7 cut(s) 936, 2106, 3363, 3834, 3968, 4333, 5242
Bsh1236I CGCG 1 cut(s) 4417
BshNI GGYRCC 1 cut(s) 734
BsiHKAI GWGCWC 2 cut(s) 2023, 3454
BsiHKCI CYCGRG 1 cut(s) 4778
BsiSI CCGG 7 cut(s) 147, 1446, 2146, 2490, 3011, 3892, 5512
BsiWI CGTACG 1 cut(s) 5476
BslFI GGGAC 4 cut(s) 453, 893, 1426, 4313
BslI CCNNNNNNNGG 6 cut(s) 146, 311, 402, 1925, 2306, 4437
BsmBI CGTCTC 3 cut(s) 12, 1905, 3648
BsmFI GGGAC 4 cut(s) 453, 893, 1426, 4313
BsmI GAATGC 3 cut(s) 1240, 1292, 2110
BsoBI CYCGRG 1 cut(s) 4778
Bsp1286I GDGCHC 2 cut(s) 2023, 3454
Bsp13I TCCGGA 3 cut(s) 3010, 3891, 5511
Bsp1407I TGTACA 2 cut(s) 1060, 5411
Bsp1720I GCTNAGC 1 cut(s) 4520
Bsp19I CCATGG 1 cut(s) 1985
BspCNI CTCAG 9 cut(s) 582, 1616, 2059, 2805, 2947, 3493, 4048, 4354, 4512
BspEI TCCGGA 3 cut(s) 3010, 3891, 5511
BspFNI CGCG 1 cut(s) 4417
BspHI TCATGA 3 cut(s) 370, 2254, 4068
BspLI GGNNCC 8 cut(s) 441, 736, 981, 2940, 4301, 4302, 4531, 5044
BspMAI CTGCAG 4 cut(s) 1618, 4056, 5014, 5020
BspMI ACCTGC 4 cut(s) 649, 2462, 4879, 5633
BspQI GCTCTTC 1 cut(s) 4977
BspT107I GGYRCC 1 cut(s) 734
BspTI CTTAAG 2 cut(s) 3445, 4967
BsrBI CCGCTC 1 cut(s) 5181
BsrGI TGTACA 2 cut(s) 1060, 5411
BsrI ACTGG 8 cut(s) 467, 684, 2657, 3941, 4891, 4916, 5044, 5701
BssECI CCNNGG 8 cut(s) 443, 1444, 1985, 2490, 2980, 3042, 3309, 4153
BssNI GRCGYC 1 cut(s) 65
BssSI CACGAG 2 cut(s) 3226, 4388
BssT1I CCWWGG 4 cut(s) 1985, 2980, 3042, 4153
Bst2BI CACGAG 2 cut(s) 3226, 4388
Bst2UI CCWGG 7 cut(s) 732, 2105, 2725, 3202, 3240, 3311, 4631
Bst6I CTCTTC 7 cut(s) 197, 2403, 2856, 3310, 4677, 4940, 4977
BstACI GRCGYC 1 cut(s) 65
BstAFI CTTAAG 2 cut(s) 3445, 4967
BstAPI GCANNNNNTGC 8 cut(s) 649, 1181, 1597, 3563, 3955, 4262, 5009, 5625
BstAUI TGTACA 2 cut(s) 1060, 5411
BstBAI YACGTR 3 cut(s) 1518, 4897, 5174
BstDSI CCRYGG 2 cut(s) 443, 1985
BstFNI CGCG 1 cut(s) 4417
BstH2I RGCGCY 1 cut(s) 419
BstHHI GCGC 4 cut(s) 418, 3917, 4417, 5526
BstNI CCWGG 7 cut(s) 732, 2105, 2725, 3202, 3240, 3311, 4631
BstNSI RCATGY 6 cut(s) 767, 1655, 1727, 2536, 4708, 4814
BstPAI GACNNNNGTC 2 cut(s) 1052, 5158
BstSCI CCNGG 9 cut(s) 730, 1444, 2103, 2489, 2723, 3200, 3238, 3309, 4629
BstSFI CTRYAG 6 cut(s) 1614, 3252, 4052, 4188, 5010, 5016
BstSNI TACGTA 1 cut(s) 4897
BstUI CGCG 1 cut(s) 4417
BstV2I GAAGAC 3 cut(s) 1499, 4653, 5363
BstX2I RGATCY 7 cut(s) 220, 979, 1115, 2117, 2843, 2938, 4177
BstXI CCANNNNNNTGG 2 cut(s) 2104, 2247
BstYI RGATCY 7 cut(s) 220, 979, 1115, 2117, 2843, 2938, 4177
BtgI CCRYGG 2 cut(s) 443, 1985
BtgZI GCGATG 3 cut(s) 165, 1221, 5221
BtrI CACGTC 1 cut(s) 5609
BtsI GCAGTG 4 cut(s) 813, 1845, 2078, 4500
BtsIMutI CAGTG 9 cut(s) 260, 813, 1351, 1845, 2078, 2840, 4500, 5084, 5162
BveI ACCTGC 4 cut(s) 649, 2462, 4879, 5633
CaiI CAGNNNCTG 3 cut(s) 473, 1844, 5009
CciI TCATGA 3 cut(s) 370, 2254, 4068
CfoI GCGC 4 cut(s) 418, 3917, 4417, 5526
CseI GACGC 3 cut(s) 54, 3665, 4053
CspCI CAANNNNNGTGG 2 cut(s) 679, 714
DraIII CACNNNGTG 2 cut(s) 1162, 2600
DrdI GACNNNNNNGTC 1 cut(s) 4242
DseDI GACNNNNNNGTC 1 cut(s) 4242
EaeI YGGCCR 4 cut(s) 297, 687, 3070, 3236
Eam1104I CTCTTC 7 cut(s) 197, 2403, 2856, 3310, 4677, 4940, 4977
EarI CTCTTC 7 cut(s) 197, 2403, 2856, 3310, 4677, 4940, 4977
EciI GGCGGA 1 cut(s) 2836
Ecl136II GAGCTC 1 cut(s) 3452
Eco105I TACGTA 1 cut(s) 4897
Eco130I CCWWGG 4 cut(s) 1985, 2980, 3042, 4153
Eco24I GRGCYC 1 cut(s) 3454
Eco32I GATATC 2 cut(s) 666, 3108
Eco47I GGWCC 9 cut(s) 440, 1769, 1859, 2965, 4235, 4300, 4529, 4827, 5160
Eco53kI GAGCTC 1 cut(s) 3452
Eco57I CTGAAG 1 cut(s) 495
Eco88I CYCGRG 1 cut(s) 4778
EcoICRI GAGCTC 1 cut(s) 3452
EcoO109I RGGNCCY 3 cut(s) 162, 4300, 4529
EcoRI GAATTC 2 cut(s) 1202, 4508
EcoRII CCWGG 7 cut(s) 730, 2103, 2723, 3200, 3238, 3309, 4629
EcoRV GATATC 2 cut(s) 666, 3108
EcoT14I CCWWGG 4 cut(s) 1985, 2980, 3042, 4153
EcoT22I ATGCAT 2 cut(s) 654, 2755
EcoT38I GRGCYC 1 cut(s) 3454
ErhI CCWWGG 4 cut(s) 1985, 2980, 3042, 4153
Esp3I CGTCTC 3 cut(s) 12, 1905, 3648
FalI AAGNNNNNCTT 8 cut(s) 1033, 1065, 1347, 1379, 4317, 4349, 5513, 5545
FaqI GGGAC 4 cut(s) 453, 893, 1426, 4313
FauI CCCGC 2 cut(s) 47, 5149
FauNDI CATATG 1 cut(s) 3181
FbaI TGATCA 1 cut(s) 3741
FblI GTMKAC 1 cut(s) 4709
FriOI GRGCYC 1 cut(s) 3454
GlaI GCGC 4 cut(s) 417, 3916, 4416, 5525
GsaI CCCAGC 1 cut(s) 2732
GsuI CTGGAG 5 cut(s) 507, 1607, 2126, 3863, 5127
HaeII RGCGCY 1 cut(s) 419
HapII CCGG 7 cut(s) 147, 1446, 2146, 2490, 3011, 3892, 5512
HgaI GACGC 3 cut(s) 54, 3665, 4053
HhaI GCGC 4 cut(s) 418, 3917, 4417, 5526
Hin1I GRCGYC 1 cut(s) 65
Hin6I GCGC 4 cut(s) 416, 3915, 4415, 5524
HinP1I GCGC 4 cut(s) 416, 3915, 4415, 5524
HincII GTYRAC 4 cut(s) 4096, 4465, 4710, 5593
HindII GTYRAC 4 cut(s) 4096, 4465, 4710, 5593
HindIII AAGCTT 4 cut(s) 233, 240, 1385, 3965
HpaII CCGG 7 cut(s) 147, 1446, 2146, 2490, 3011, 3892, 5512
HphI GGTGA 8 cut(s) 385, 673, 1806, 1940, 2612, 2750, 2996, 4860
Hpy99I CGWCG 3 cut(s) 70, 82, 119
HpyCH4IV ACGT 9 cut(s) 114, 906, 1439, 1517, 1898, 4896, 5026, 5173, 5608
HpySE526I ACGT 9 cut(s) 114, 906, 1439, 1517, 1898, 4896, 5026, 5173, 5608
Hsp92I GRCGYC 1 cut(s) 65
HspAI GCGC 4 cut(s) 416, 3915, 4415, 5524
KflI GGGWCCC 1 cut(s) 4300
Kpn2I TCCGGA 3 cut(s) 3010, 3891, 5511
Ksp22I TGATCA 1 cut(s) 3741
LguI GCTCTTC 1 cut(s) 4977
MaeII ACGT 9 cut(s) 114, 906, 1439, 1517, 1898, 4896, 5026, 5173, 5608
MbiI CCGCTC 1 cut(s) 5181
MfeI CAATTG 1 cut(s) 3059
MflI RGATCY 7 cut(s) 220, 979, 1115, 2117, 2843, 2938, 4177
MhlI GDGCHC 2 cut(s) 2023, 3454
MlsI TGGCCA 3 cut(s) 689, 3072, 3238
MluNI TGGCCA 3 cut(s) 689, 3072, 3238
MlyI GAGTC 3 cut(s) 3187, 3638, 5428
MmeI TCCRAC 6 cut(s) 97, 1025, 1509, 1747, 3529, 3974
Mox20I TGGCCA 3 cut(s) 689, 3072, 3238
Mph1103I ATGCAT 2 cut(s) 654, 2755
MroI TCCGGA 3 cut(s) 3010, 3891, 5511
MroXI GAANNNNTTC 4 cut(s) 1290, 2745, 4986, 5041
MscI TGGCCA 3 cut(s) 689, 3072, 3238
MslI CAYNNNNRTG 4 cut(s) 1000, 2228, 2758, 2893
Msp20I TGGCCA 3 cut(s) 689, 3072, 3238
MspA1I CMGCKG 5 cut(s) 424, 3787, 3923, 4412, 5015
MspCI CTTAAG 2 cut(s) 3445, 4967
MspI CCGG 7 cut(s) 147, 1446, 2146, 2490, 3011, 3892, 5512
MspR9I CCNGG 9 cut(s) 732, 1446, 2105, 2491, 2725, 3202, 3240, 3311, 4631
MunI CAATTG 1 cut(s) 3059
Mva1269I GAATGC 3 cut(s) 1240, 1292, 2110
MvaI CCWGG 7 cut(s) 732, 2105, 2725, 3202, 3240, 3311, 4631
MvnI CGCG 1 cut(s) 4417
NciI CCSGG 2 cut(s) 1446, 2491
NcoI CCATGG 1 cut(s) 1985
NdeI CATATG 1 cut(s) 3181
NlaIV GGNNCC 8 cut(s) 441, 736, 981, 2940, 4301, 4302, 4531, 5044
NmuCI GTSAC 3 cut(s) 391, 4385, 5174
NsiI ATGCAT 2 cut(s) 654, 2755
NspI RCATGY 6 cut(s) 767, 1655, 1727, 2536, 4708, 4814
PaeI GCATGC 1 cut(s) 1655
PaeR7I CTCGAG 1 cut(s) 4778
PagI TCATGA 3 cut(s) 370, 2254, 4068
PaqCI CACCTGC 2 cut(s) 649, 4879
PciI ACATGT 2 cut(s) 4704, 4810
PciSI GCTCTTC 1 cut(s) 4977
PcsI WCGNNNNNNNCGW 2 cut(s) 74, 111
PctI GAATGC 3 cut(s) 1240, 1292, 2110
PdmI GAANNNNTTC 4 cut(s) 1290, 2745, 4986, 5041
Pfl23II CGTACG 1 cut(s) 5476
PflFI GACNNNGTC 1 cut(s) 467
PleI GAGTC 3 cut(s) 3187, 3638, 5427
PpsI GAGTC 3 cut(s) 3187, 3638, 5427
Ppu21I YACGTR 3 cut(s) 1518, 4897, 5174
PpuMI RGGWCCY 2 cut(s) 4300, 4529
PscI ACATGT 2 cut(s) 4704, 4810
PshAI GACNNNNGTC 2 cut(s) 1052, 5158
PshBI ATTAAT 1 cut(s) 3930
PsiI TTATAA 1 cut(s) 899
Psp124BI GAGCTC 1 cut(s) 3454
Psp5II RGGWCCY 2 cut(s) 4300, 4529
Psp6I CCWGG 7 cut(s) 730, 2103, 2723, 3200, 3238, 3309, 4629
PspFI CCCAGC 1 cut(s) 2728
PspGI CCWGG 7 cut(s) 730, 2103, 2723, 3200, 3238, 3309, 4629
PspLI CGTACG 1 cut(s) 5476
PspN4I GGNNCC 8 cut(s) 441, 736, 981, 2940, 4301, 4302, 4531, 5044
PspPPI RGGWCCY 2 cut(s) 4300, 4529
PsrI GAACNNNNNNTAC 2 cut(s) 4608, 4640
PstI CTGCAG 4 cut(s) 1618, 4056, 5014, 5020
PstNI CAGNNNCTG 3 cut(s) 473, 1844, 5009
PsuI RGATCY 7 cut(s) 220, 979, 1115, 2117, 2843, 2938, 4177
PsyI GACNNNGTC 1 cut(s) 467
PvuII CAGCTG 4 cut(s) 424, 3787, 3923, 5015
RseI CAYNNNNRTG 4 cut(s) 1000, 2228, 2758, 2893
SacI GAGCTC 1 cut(s) 3454
SalI GTCGAC 1 cut(s) 4708
SapI GCTCTTC 1 cut(s) 4977
ScaI AGTACT 1 cut(s) 2565
SchI GAGTC 3 cut(s) 3187, 3638, 5428
ScrFI CCNGG 9 cut(s) 732, 1446, 2105, 2491, 2725, 3202, 3240, 3311, 4631
SduI GDGCHC 2 cut(s) 2023, 3454
SfcI CTRYAG 6 cut(s) 1614, 3252, 4052, 4188, 5010, 5016
Sfr274I CTCGAG 1 cut(s) 4778
SinI GGWCC 9 cut(s) 440, 1769, 1859, 2965, 4235, 4300, 4529, 4827, 5160
SlaI CTCGAG 1 cut(s) 4778
SmiMI CAYNNNNRTG 4 cut(s) 1000, 2228, 2758, 2893
SmlI CTYRAG 7 cut(s) 483, 912, 1315, 1420, 3445, 4778, 4967
SmoI CTYRAG 7 cut(s) 483, 912, 1315, 1420, 3445, 4778, 4967
SnaBI TACGTA 1 cut(s) 4897
SpeI ACTAGT 1 cut(s) 4885
SphI GCATGC 1 cut(s) 1655
SspI AATATT 4 cut(s) 827, 2060, 3219, 4923
SstI GAGCTC 1 cut(s) 3454
StyD4I CCNGG 9 cut(s) 730, 1444, 2103, 2489, 2723, 3200, 3238, 3309, 4629
StyI CCWWGG 4 cut(s) 1985, 2980, 3042, 4153
TaiI ACGT 9 cut(s) 117, 909, 1442, 1520, 1901, 4899, 5029, 5176, 5611
TatI WGTACW 5 cut(s) 1060, 1072, 2333, 2563, 5411
TauI GCSGC 6 cut(s) 65, 95, 299, 361, 2577, 4415
TscAI CASTG 9 cut(s) 267, 820, 1351, 1845, 2078, 2840, 4500, 5091, 5162
TseFI GTSAC 3 cut(s) 391, 4385, 5174
Tsp45I GTSAC 3 cut(s) 391, 4385, 5174
TspGWI ACGGA 6 cut(s) 36, 854, 2202, 4733, 5357, 5494
TspRI CASTG 9 cut(s) 267, 820, 1351, 1845, 2078, 2840, 4500, 5091, 5162
Tth111I GACNNNGTC 1 cut(s) 467
Vha464I CTTAAG 2 cut(s) 3445, 4967
VpaK11BI GGWCC 9 cut(s) 440, 1769, 1859, 2965, 4235, 4300, 4529, 4827, 5160
VspI ATTAAT 1 cut(s) 3930
XbaI TCTAGA 2 cut(s) 2263, 4951
XceI RCATGY 6 cut(s) 767, 1655, 1727, 2536, 4708, 4814
XcmI CCANNNNNNNNNTGG 1 cut(s) 3065
XhoI CTCGAG 1 cut(s) 4778
XmiI GTMKAC 1 cut(s) 4709
XmnI GAANNNNTTC 4 cut(s) 1290, 2745, 4986, 5041
ZrmI AGTACT 1 cut(s) 2565
Zsp2I ATGCAT 2 cut(s) 654, 2755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.